Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,086,004 C→A 85.6% A42A (GCC→GCA)  hisC → histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,086,0040CA85.6% 333.0 / 60.4 151A42A (GCC→GCA) hisChistidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (10/12);  new base A (60/69);  total (70/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 151 total aligned reads displayed.

CCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTG  >  CP009273/2085863‑2086148
                                                                                                                                             |                                                                                                                                                
cccTTAAGGAGCAAGCATGAGCACCGTGACTATTACCTTTTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAg                                                                                                                                            <  2:17099/148‑1 (MQ=255)
   ttAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGttt                                                                                                                                         <  2:77956/148‑1 (MQ=255)
   ttAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGttt                                                                                                                                         <  2:77925/148‑1 (MQ=255)
    tAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTc                                                                                                                                        <  2:2644629/148‑1 (MQ=255)
    tAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTc                                                                                                                                        >  2:3160762/1‑148 (MQ=255)
    tAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTc                                                                                                                                        <  2:2146396/148‑1 (MQ=255)
    tAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTc                                                                                                                                        >  2:1863470/1‑148 (MQ=255)
    tAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTc                                                                                                                                        <  2:397700/148‑1 (MQ=255)
     aaGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCa                                                                                                                                       >  2:1659998/1‑148 (MQ=255)
     aaGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCa                                                                                                                                       >  2:1659997/1‑148 (MQ=255)
      aGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAACACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAg                                                                                                                                      <  2:2822650/148‑1 (MQ=255)
      aGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAg                                                                                                                                      <  2:2822655/148‑1 (MQ=255)
        gAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCt                                                                                                                                    <  1:2768482/148‑1 (MQ=255)
        gAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCt                                                                                                                                    >  2:2498039/1‑148 (MQ=255)
        gAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCt                                                                                                                                    >  2:2497887/1‑148 (MQ=255)
        gAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCt                                                                                                                                    <  1:2257884/148‑1 (MQ=255)
         agcaagcaTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCtt                                                                                                                                   <  2:2369593/148‑1 (MQ=255)
          gcaagcaTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTa                                                                                                                                  >  1:3246066/1‑148 (MQ=255)
           caagcaTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTAc                                                                                                                                 >  1:2693972/1‑148 (MQ=255)
              gcaTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCa                                                                                                                              >  1:2583388/1‑148 (MQ=255)
              gcaTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCa                                                                                                                              >  2:1683137/1‑148 (MQ=255)
               caTGAGCACCGAGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGAGCGTCGTCTGGGCGGTAACGGCTATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAg                                                                                                                             >  2:2086846/1‑148 (MQ=255)
                 tGAGCACCGTGACTATTCCCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCa                                                                                                                           <  2:573092/148‑1 (MQ=255)
                        cGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTc                                                                                                                    >  1:1304020/1‑148 (MQ=255)
                          tGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCaa                                                                                                                  <  1:1090890/148‑1 (MQ=255)
                          tGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCaa                                                                                                                  <  1:1092342/148‑1 (MQ=255)
                           gACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAAc                                                                                                                 >  2:1165729/1‑148 (MQ=255)
                           gACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAAc                                                                                                                 >  2:1520443/1‑148 (MQ=255)
                           gACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAAc                                                                                                                 >  2:817740/1‑148 (MQ=255)
                               aTTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCt                                                                                                             >  2:2734073/1‑148 (MQ=255)
                               aTTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCt                                                                                                             <  2:2901632/148‑1 (MQ=255)
                                 tACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTAc                                                                                                           >  1:2880976/1‑148 (MQ=255)
                                 tACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTAc                                                                                                           >  2:1938031/1‑148 (MQ=255)
                                 tACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTAc                                                                                                           >  2:2192633/1‑148 (MQ=255)
                                   ccGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTAccc                                                                                                         >  2:1119843/1‑148 (MQ=255)
                                   ccGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTAccc                                                                                                         >  2:1545190/1‑148 (MQ=255)
                                     gATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCgg                                                                                                       <  1:2941047/148‑1 (MQ=255)
                                     gATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCgg                                                                                                       <  1:3217394/148‑1 (MQ=255)
                                       tttAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGaa                                                                                                     >  1:1019594/1‑148 (MQ=255)
                                       tttAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGaa                                                                                                     >  2:1805468/1‑148 (MQ=255)
                                       tttAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGaa                                                                                                     <  2:357369/148‑1 (MQ=255)
                                        ttAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAAt                                                                                                    >  2:2068726/1‑148 (MQ=255)
                                           gcgcGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGcc                                                                                                 <  2:2717568/148‑1 (MQ=255)
                                                   aaaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGaaa                                                                                         <  2:938100/148‑1 (MQ=255)
                                                   aaaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGaaa                                                                                         >  1:862166/1‑148 (MQ=255)
                                                   aaaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGaaa                                                                                         >  1:2967103/1‑148 (MQ=255)
                                                    aaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAg                                                                                        >  2:2534195/1‑148 (MQ=255)
                                                         ccGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCAACTGCAGTGGAGTTTCAGCTTCCTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTg                                                                                   >  2:1700999/1‑148 (MQ=255)
                                                         ccGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCAACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTg                                                                                   >  2:3189798/1‑148 (MQ=255)
                                                          cGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGa                                                                                  <  2:2904081/148‑1 (MQ=255)
                                                          cGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGa                                                                                  <  2:1508989/148‑1 (MQ=255)
                                                           gCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGAt                                                                                 <  2:2362974/148‑1 (MQ=255)
                                                           gCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGAt                                                                                 <  1:2534195/148‑1 (MQ=255)
                                                              aCCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGa                                                                              <  1:274867/148‑1 (MQ=255)
                                                              aCCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGa                                                                              <  1:274775/148‑1 (MQ=255)
                                                                 tGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGaaaa                                                                           >  2:3170354/1‑148 (MQ=255)
                                                                     gCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTAc                                                                       >  1:2246290/1‑148 (MQ=255)
                                                                      ccGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTAcg                                                                      <  1:606916/148‑1 (MQ=255)
                                                                      ccGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTAcg                                                                      <  1:1742429/148‑1 (MQ=255)
                                                                        gTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTAcgcg                                                                    <  1:730450/148‑1 (MQ=255)
                                                                          aTCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCa                                                                  <  2:227899/148‑1 (MQ=255)
                                                                          aTCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCa                                                                  <  2:33089/148‑1 (MQ=255)
                                                                            cAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAat                                                                >  2:1053218/1‑148 (MQ=255)
                                                                                     cgtcgtCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCg                                                       >  1:807518/1‑148 (MQ=255)
                                                                                         gtcTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTaaa                                                   <  1:2455459/148‑1 (MQ=255)
                                                                                         gtcTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTaaa                                                   <  1:796597/148‑1 (MQ=255)
                                                                                          tcTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTaaaa                                                  >  2:1477695/1‑148 (MQ=255)
                                                                                            tGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAAcc                                                >  1:2674400/1‑148 (MQ=255)
                                                                                            tGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAAcc                                                >  1:1525194/1‑148 (MQ=255)
                                                                                             gggCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCg                                               >  1:1059035/1‑148 (MQ=255)
                                                                                              ggCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCgg                                              >  1:2388933/1‑148 (MQ=255)
                                                                                              ggCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCgg                                              <  1:1165729/148‑1 (MQ=255)
                                                                                              ggCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCgg                                              <  2:341982/148‑1 (MQ=255)
                                                                                              ggCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCgg                                              <  2:341798/148‑1 (MQ=255)
                                                                                                  gTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCa                                          <  1:431032/148‑1 (MQ=255)
                                                                                                  gTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCa                                          <  1:431011/148‑1 (MQ=255)
                                                                                                   tAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAg                                         <  2:3222607/148‑1 (MQ=255)
                                                                                                    aaCGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAgg                                        <  1:1998558/148‑1 (MQ=255)
                                                                                                     aCGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGt                                       >  1:1863377/1‑148 (MQ=255)
                                                                                                      cGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTg                                      >  2:1718793/1‑148 (MQ=255)
                                                                                                         cGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTg                                   >  1:84827/1‑148 (MQ=255)
                                                                                                            tGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTc                                <  1:577733/148‑1 (MQ=255)
                                                                                                                 ggCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCg                           <  2:1402780/148‑1 (MQ=255)
                                                                                                                  gCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGt                          >  1:804486/1‑148 (MQ=255)
                                                                                                                   cTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTg                         >  2:831329/1‑148 (MQ=255)
                                                                                                                         gCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCgg                   <  2:185591/148‑1 (MQ=255)
                                                                                                                              cGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGaa              >  1:1945405/1‑148 (MQ=255)
                                                                                                                                 aTACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGt           <  1:2291612/148‑1 (MQ=255)
                                                                                                                                 aTACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGt           >  1:3177186/1‑148 (MQ=255)
                                                                                                                                 aTACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGt           <  1:1940008/148‑1 (MQ=255)
                                                                                                                                 aTACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGNGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGt           <  1:1683137/148‑1 (MQ=255)
                                                                                                                                  tACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTa          <  2:2951238/148‑1 (MQ=255)
                                                                                                                                  tACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTa          <  2:2951234/148‑1 (MQ=255)
                                                                                                                                      ccACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGa      >  1:3024102/1‑148 (MQ=255)
                                                                                                                                      ccACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGa      >  1:3027169/1‑148 (MQ=255)
                                                                                                                                      ccACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGa      <  1:1805468/148‑1 (MQ=255)
                                                                                                                                       cACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGaa     >  1:1398673/1‑148 (MQ=255)
                                                                                                                                        aCTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAACGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAAc    <  2:230122/148‑1 (MQ=255)
                                                                                                                                        aCTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAAc    >  1:1983584/1‑148 (MQ=255)
                                                                                                                                          tGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAActg  >  1:718135/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                
CCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTG  >  CP009273/2085863‑2086148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTGCTGA  >  CP009273/2085871‑2086152
                                                                                                                                     |                                                                                                                                                    
GAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTA                                                                                                                                      <  SRR19973070.2786203/150‑1 (MQ=60)
GAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTA                                                                                                                                      <  SRR19973070.2272859/150‑1 (MQ=60)
GAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTA                                                                                                                                      >  SRR19973070.3266986/1‑150 (MQ=60)
 AGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTAC                                                                                                                                     >  SRR19973070.2711420/1‑150 (MQ=60)
    AAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCA                                                                                                                                  >  SRR19973070.2600273/1‑150 (MQ=60)
    AAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCA                                                                                                                                  >  SRR19973070.2599294/1‑150 (MQ=60)
              ACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTC                                                                                                                        >  SRR19973070.1313082/1‑150 (MQ=60)
                  TGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACC                                                                                                                    <  SRR19973070.1098429/150‑1 (MQ=60)
                  TGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACC                                                                                                                    <  SRR19973070.1099884/150‑1 (MQ=60)
                       ATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTAC                                                                                                               >  SRR19973070.2898453/1‑150 (MQ=60)
                       ATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTAC                                                                                                               >  SRR19973070.2899627/1‑150 (MQ=60)
                        TTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACC                                                                                                              <  SRR19973070.1349256/150‑1 (MQ=60)
                             GATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAA                                                                                                         <  SRR19973070.3238196/150‑1 (MQ=60)
                             GATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAA                                                                                                         <  SRR19973070.2959998/150‑1 (MQ=60)
                             GATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAA                                                                                                         >  SRR19973070.1026835/1‑150 (MQ=60)
                                      CGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCG                                                                                                <  SRR19973070.1162404/150‑1 (MQ=60)
                                         GAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAA                                                                                             >  SRR19973070.868159/1‑150 (MQ=60)
                                         GAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAA                                                                                             >  SRR19973070.2986169/1‑150 (MQ=60)
                                         GAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAA                                                                                             >  SRR19973070.2985983/1‑150 (MQ=60)
                                            AACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCG                                                                                          <  SRR19973070.1687811/150‑1 (MQ=60)
                                                   GCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTG                                                                                   <  SRR19973070.2550757/150‑1 (MQ=60)
                                                    CAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCGACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGA                                                                                  >  SRR19973070.1965469/1‑150 (MQ=60)
                                                      ACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAA                                                                                <  SRR19973070.276564/150‑1 (MQ=60)
                                                      ACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAA                                                                                <  SRR19973070.276651/150‑1 (MQ=60)
                                                      ACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAA                                                                                <  SRR19973070.276657/150‑1 (MQ=60)
                                                           ACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGACTGCCAGCCGAAAGCGGTGATTGAAAATTAC                                                                           >  SRR19973070.1801957/1‑150 (MQ=60)
                                                           ACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTAC                                                                           >  SRR19973070.2261142/1‑150 (MQ=60)
                                                              CCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCG                                                                        <  SRR19973070.611162/150‑1 (MQ=60)
                                                              CCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCG                                                                        <  SRR19973070.1754511/150‑1 (MQ=60)
                                                                GTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCA                                                                      <  SRR19973070.735630/150‑1 (MQ=60)
                                                                           CGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCG                                                           >  SRR19973070.813059/1‑150 (MQ=60)
                                                                               TCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAA                                                       <  SRR19973070.630814/150‑1 (MQ=60)
                                                                                 GTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAAC                                                     <  SRR19973070.802098/150‑1 (MQ=60)
                                                                                 GTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAAC                                                     <  SRR19973070.2471510/150‑1 (MQ=60)
                                                                                  TCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACC                                                    >  SRR19973070.1535903/1‑150 (MQ=60)
                                                                                  TCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACC                                                    >  SRR19973070.2691764/1‑150 (MQ=60)
                                                                                   CTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCG                                                   >  SRR19973070.1066468/1‑150 (MQ=60)
                                                                                   CTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCG                                                   >  SRR19973070.1066601/1‑150 (MQ=60)
                                                                                    TGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGG                                                  >  SRR19973070.2404711/1‑150 (MQ=60)
                                                                                    TGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGG                                                  >  SRR19973070.2404709/1‑150 (MQ=60)
                                                                                      GGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAG                                                <  SRR19973070.1173893/150‑1 (MQ=60)
                                                                                          GTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGG                                            <  SRR19973070.544642/150‑1 (MQ=60)
                                                                                          GTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGG                                            <  SRR19973070.434166/150‑1 (MQ=60)
                                                                                          GTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGG                                            <  SRR19973070.434145/150‑1 (MQ=60)
                                                                                           TAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGT                                           >  SRR19973070.762181/1‑150 (MQ=60)
                                                                                           TAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGT                                           >  SRR19973070.1876118/1‑150 (MQ=60)
                                                                                           TAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGT                                           >  SRR19973070.1876126/1‑150 (MQ=60)
                                                                                            AACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTG                                          >  SRR19973070.2073110/1‑150 (MQ=60)
                                                                                            AACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTG                                          <  SRR19973070.2012094/150‑1 (MQ=60)
                                                                                               GGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCTGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTG                                       <  SRR19973070.2693994/150‑1 (MQ=60)
                                                                                               GGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTG                                       >  SRR19973070.85712/1‑150 (MQ=60)
                                                                                               GGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTG                                       >  SRR19973070.85692/1‑150 (MQ=60)
                                                                                                    TGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAG                                  <  SRR19973070.581697/150‑1 (MQ=60)
                                                                                                    TGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAG                                  <  SRR19973070.3237885/150‑1 (MQ=60)
                                                                                                        TGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGT                              >  SRR19973070.810031/1‑150 (MQ=60)
                                                                                                        TGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGT                              >  SRR19973070.810021/1‑150 (MQ=60)
                                                                                                                    AACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAA                  >  SRR19973070.1958468/1‑150 (MQ=60)
                                                                                                                     ACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAG                 <  SRR19973070.3289811/150‑1 (MQ=60)
                                                                                                                       GAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGT               >  SRR19973070.3197752/1‑150 (MQ=60)
                                                                                                                         ATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTAT             <  SRR19973070.2306851/150‑1 (MQ=60)
                                                                                                                         ATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTAT             <  SRR19973070.2306847/150‑1 (MQ=60)
                                                                                                                         ATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTAT             <  SRR19973070.1953048/150‑1 (MQ=60)
                                                                                                                         ATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGNGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTAT             <  SRR19973070.1694679/150‑1 (MQ=60)
                                                                                                                            CCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGA          >  SRR19973070.3043369/1‑150 (MQ=60)
                                                                                                                            CCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGA          >  SRR19973070.3043445/1‑150 (MQ=60)
                                                                                                                            CCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGA          >  SRR19973070.3046447/1‑150 (MQ=60)
                                                                                                                             CCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAA         >  SRR19973070.1408092/1‑150 (MQ=60)
                                                                                                                              CCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAAC        >  SRR19973070.1997011/1‑150 (MQ=60)
                                                                                                                              CCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAAC        <  SRR19973070.1817930/150‑1 (MQ=60)
                                                                                                                                ACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTG      >  SRR19973070.723256/1‑150 (MQ=60)
                                                                                                                                   GCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTGCTG   <  SRR19973070.1530168/150‑1 (MQ=60)
                                                                                                                                    CAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTGCTGA  >  SRR19973070.535800/1‑150 (MQ=60)
                                                                                                                                     |                                                                                                                                                    
GAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTGCTGA  >  CP009273/2085871‑2086152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑