Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,086,004 C→A 86.7% A42A (GCC→GCA)  hisC → histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,086,0040CA86.7% 644.8 / 97.0 267A42A (GCC→GCA) hisChistidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (19/17);  new base A (97/134);  total (116/151)
Fisher's exact test for biased strand distribution p-value = 2.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

AACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTGC  >  CP009273/2085859‑2086149
                                                                                                                                                 |                                                                                                                                                 
 aCGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTgg                                                                                                                                               <  2:3843152/149‑1 (MQ=255)
  cGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGa                                                                                                                                              <  1:4644121/149‑1 (MQ=255)
   gCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAg                                                                                                                                             >  2:83174/1‑149 (MQ=255)
   gCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAg                                                                                                                                             >  2:4664937/1‑149 (MQ=255)
   gCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAg                                                                                                                                             >  2:470592/1‑149 (MQ=255)
    cccTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGt                                                                                                                                            >  2:3615225/1‑149 (MQ=255)
    cccTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGt                                                                                                                                            >  2:739519/1‑149 (MQ=255)
     ccTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGtt                                                                                                                                           <  2:4468274/149‑1 (MQ=255)
     ccTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGtt                                                                                                                                           >  2:4532755/1‑149 (MQ=255)
       ttAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGTCGTGGAGTTTc                                                                                                                                         <  1:1006269/149‑1 (MQ=255)
       ttAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTc                                                                                                                                         <  1:2921459/149‑1 (MQ=255)
        tAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCa                                                                                                                                        <  2:1214785/149‑1 (MQ=255)
         aaGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAg                                                                                                                                       >  2:1475773/1‑149 (MQ=255)
         aaGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAg                                                                                                                                       <  2:4420091/149‑1 (MQ=255)
          aGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGc                                                                                                                                      >  2:941759/1‑149 (MQ=255)
           ggAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCt                                                                                                                                     >  2:1236972/1‑149 (MQ=255)
              gcaagcaTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTAc                                                                                                                                  >  2:3378879/1‑149 (MQ=255)
                aagcaTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTc                                                                                                                                >  1:451786/1‑149 (MQ=255)
                 agcgTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCa                                                                                                                               >  1:1931129/1‑149 (MQ=255)
                 agcaTGAGCACCGTGACTATTACCGCTTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCa                                                                                                                               <  2:785519/149‑1 (MQ=255)
                 agcaTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCa                                                                                                                               <  2:2106994/149‑1 (MQ=255)
                   caTGAGCACGGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGc                                                                                                                             <  1:2925687/149‑1 (MQ=255)
                    aTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCa                                                                                                                            >  1:38963/1‑149 (MQ=255)
                    aTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCa                                                                                                                            >  1:2420530/1‑149 (MQ=255)
                      gAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCaaa                                                                                                                          >  2:1769405/1‑149 (MQ=255)
                       aGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAAc                                                                                                                         <  2:4297153/149‑1 (MQ=255)
                        gCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACg                                                                                                                        >  1:1538603/1‑149 (MQ=255)
                          aCCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCt                                                                                                                      >  1:1484591/1‑149 (MQ=255)
                          aCCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCt                                                                                                                      <  2:620261/149‑1 (MQ=255)
                          aCCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCt                                                                                                                      >  2:4752554/1‑149 (MQ=255)
                           ccGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTc                                                                                                                     <  1:244678/149‑1 (MQ=255)
                               gACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAATCGCTCAAcc                                                                                                                 <  2:2354819/149‑1 (MQ=255)
                               gACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAAcc                                                                                                                 >  2:541691/1‑149 (MQ=255)
                                 cTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGc                                                                                                               <  2:4240175/149‑1 (MQ=255)
                                      aCCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTAccc                                                                                                          >  1:3334506/1‑149 (MQ=255)
                                          aTTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGaa                                                                                                      <  2:4075420/149‑1 (MQ=255)
                                           tttAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAAt                                                                                                     >  2:532693/1‑149 (MQ=255)
                                               gcgcGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCa                                                                                                 <  2:1558970/149‑1 (MQ=255)
                                               gcgcGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCa                                                                                                 <  2:2815772/149‑1 (MQ=255)
                                                  cgTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGcc                                                                                              <  1:2876455/149‑1 (MQ=255)
                                                     gAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGaa                                                                                           >  2:3050573/1‑149 (MQ=255)
                                                       aaaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAg                                                                                         >  1:2095674/1‑149 (MQ=255)
                                                       aaaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAg                                                                                         >  2:4204722/1‑149 (MQ=255)
                                                        aaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGc                                                                                        <  1:4270312/149‑1 (MQ=255)
                                                        aaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGc                                                                                        <  1:3585351/149‑1 (MQ=255)
                                                           gTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGt                                                                                     <  2:2870542/149‑1 (MQ=255)
                                                           gTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGt                                                                                     >  1:1139312/1‑149 (MQ=255)
                                                           gTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGt                                                                                     >  1:1877064/1‑149 (MQ=255)
                                                             ccGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGa                                                                                   <  1:162300/149‑1 (MQ=255)
                                                              cGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGAt                                                                                  <  1:3394746/149‑1 (MQ=255)
                                                                cAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTg                                                                                >  2:1488833/1‑149 (MQ=255)
                                                                cAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTg                                                                                <  1:1708445/149‑1 (MQ=255)
                                                                cAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTg                                                                                <  2:2578389/149‑1 (MQ=255)
                                                                  aCCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGaa                                                                              >  2:295911/1‑149 (MQ=255)
                                                                   ccTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGaaa                                                                             <  1:3630055/149‑1 (MQ=255)
                                                                      gACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAAtt                                                                          <  2:2137394/149‑1 (MQ=255)
                                                                        cGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTAc                                                                        <  1:3778664/149‑1 (MQ=255)
                                                                           cGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTAcgcg                                                                     <  1:4206041/149‑1 (MQ=255)
                                                                            gTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACgcgc                                                                    <  2:2497742/149‑1 (MQ=255)
                                                                                cAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAata                                                                <  2:2671351/149‑1 (MQ=255)
                                                                                    cGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGTCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCa                                                            <  2:3514031/149‑1 (MQ=255)
                                                                                     ggCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAg                                                           <  1:1784972/149‑1 (MQ=255)
                                                                                     ggCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAg                                                           <  2:3236978/149‑1 (MQ=255)
                                                                                       cgcgTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGc                                                         <  1:318338/149‑1 (MQ=255)
                                                                                        gcgTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCg                                                        >  2:3943838/1‑149 (MQ=255)
                                                                                           tcgtcTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTaa                                                     >  1:4479549/1‑149 (MQ=255)
                                                                                              tcTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAAc                                                  >  2:1418204/1‑149 (MQ=255)
                                                                                                    cGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGGGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGc                                            >  1:781911/1‑149 (MQ=255)
                                                                                                         aCGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTAAGCGCAATATGCAGGCGTAAAACCGGAGCAGGTg                                       >  1:593283/1‑149 (MQ=255)
                                                                                                         aCGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAACGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTg                                       >  2:795273/1‑149 (MQ=255)
                                                                                                         aCGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTg                                       >  1:2279039/1‑149 (MQ=255)
                                                                                                          cGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGc                                      >  1:623916/1‑149 (MQ=255)
                                                                                                            gCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTg                                    >  2:4682117/1‑149 (MQ=255)
                                                                                                            gCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTg                                    >  2:2298534/1‑149 (MQ=255)
                                                                                                            gCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCACTATGCAGGCGTAAAACCGGAGCAGGTGCTg                                    >  1:1850065/1‑149 (MQ=255)
                                                                                                               aTGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTc                                 >  1:2222063/1‑149 (MQ=255)
                                                                                                                cgTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCa                                <  2:4776717/148‑1 (MQ=255)
                                                                                                                 gTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAg                               >  2:4128802/1‑149 (MQ=255)
                                                                                                                       cTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTgg                         <  2:4364077/149‑1 (MQ=255)
                                                                                                                        tGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGgc                        >  2:3449990/1‑149 (MQ=255)
                                                                                                                         gAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGgcg                       >  2:3876620/1‑149 (MQ=255)
                                                                                                                         gAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGgcg                       >  1:2205617/1‑149 (MQ=255)
                                                                                                                           aCGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGcgcg                     >  1:435295/1‑149 (MQ=255)
                                                                                                                           aCGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGcgcg                     >  2:186744/1‑149 (MQ=255)
                                                                                                                            cGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCgg                    <  2:3566567/149‑1 (MQ=255)
                                                                                                                             gCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGa                   <  1:749726/149‑1 (MQ=255)
                                                                                                                              ccAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGAc                  >  1:2559504/1‑149 (MQ=255)
                                                                                                                                 aCGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGaa               <  2:2021736/149‑1 (MQ=255)
                                                                                                                                   gAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAgg             >  2:3070010/1‑149 (MQ=255)
                                                                                                                                     aTACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGtc           >  2:4287853/1‑148 (MQ=255)
                                                                                                                                     aTACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTa           >  2:2289831/1‑149 (MQ=255)
                                                                                                                                     aTACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTa           <  1:541412/149‑1 (MQ=255)
                                                                                                                                      tACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTAt          >  1:894520/1‑149 (MQ=255)
                                                                                                                                       aCCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTAtt         <  1:661987/149‑1 (MQ=255)
                                                                                                                                       aCCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTAtt         <  2:1141794/149‑1 (MQ=255)
                                                                                                                                         cccACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGa       >  1:118371/1‑149 (MQ=255)
                                                                                                                                          ccACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGaa      <  1:4073792/149‑1 (MQ=255)
                                                                                                                                          ccACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGaa      <  1:4442683/149‑1 (MQ=255)
                                                                                                                                          ccACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGaa      <  1:3447329/149‑1 (MQ=255)
                                                                                                                                              tGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAActgc  <  2:2275224/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTGC  >  CP009273/2085859‑2086149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTGCTGAT  >  CP009273/2085855‑2086153
                                                                                                                                                     |                                                                                                                                                     
TGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCA                                                                                                                                                       >  SRR19973071.2254397/1‑150 (MQ=60)
TGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCA                                                                                                                                                       >  SRR19973071.3127420/1‑150 (MQ=60)
  TTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGT                                                                                                                                                     <  SRR19973071.2201747/150‑1 (MQ=60)
      CGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGTTTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAG                                                                                                                                                 <  SRR19973071.437235/150‑1 (MQ=60)
      CGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAG                                                                                                                                                 <  SRR19973071.4660662/150‑1 (MQ=60)
           TTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTCGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCA                                                                                                                                            <  SRR19973071.2932382/150‑1 (MQ=60)
           TTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAACACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCA                                                                                                                                            <  SRR19973071.3485774/150‑1 (MQ=60)
           TTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGTCGTGGAGTTTCA                                                                                                                                            <  SRR19973071.1009925/150‑1 (MQ=60)
           TTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCA                                                                                                                                            <  SRR19973071.2931919/150‑1 (MQ=60)
           TTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCA                                                                                                                                            <  SRR19973071.2931930/150‑1 (MQ=60)
                  GCAAGCATGAGCACCGTGACTATTCCCGATTTCGCGGGTGAACACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACT                                                                                                                                     <  SRR19973071.538108/150‑1 (MQ=60)
                  GCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACT                                                                                                                                     <  SRR19973071.2880423/150‑1 (MQ=60)
                   CAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTC                                                                                                                                    >  SRR19973071.453412/1‑150 (MQ=60)
                    AAGCGTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCA                                                                                                                                   >  SRR19973071.1938168/1‑150 (MQ=60)
                       CATGAGCACGGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCA                                                                                                                                <  SRR19973071.2936170/150‑1 (MQ=60)
                       CATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCA                                                                                                                                >  SRR19973071.39208/1‑150 (MQ=60)
                       CATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCA                                                                                                                                <  SRR19973071.2936084/150‑1 (MQ=60)
                       CATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCA                                                                                                                                >  SRR19973071.2429279/1‑150 (MQ=60)
                        ATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAA                                                                                                                               >  SRR19973071.2283532/1‑150 (MQ=60)
                        ATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAA                                                                                                                               >  SRR19973071.2283585/1‑150 (MQ=60)
                          GAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAAC                                                                                                                             <  SRR19973071.3123236/150‑1 (MQ=60)
                          GAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAAC                                                                                                                             <  SRR19973071.4289762/150‑1 (MQ=60)
                           AGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACG                                                                                                                            >  SRR19973071.1544120/1‑150 (MQ=60)
                             CACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCT                                                                                                                          >  SRR19973071.1489985/1‑150 (MQ=60)
                               CCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCA                                                                                                                        <  SRR19973071.245612/150‑1 (MQ=60)
                                CGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAA                                                                                                                       <  SRR19973071.2687916/150‑1 (MQ=60)
                                         TACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCC                                                                                                              >  SRR19973071.3346315/1‑150 (MQ=60)
                                          cactatttcgcgtGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCG                                                                                                             <  SRR19973071.1340491/137‑1 (MQ=60)
                                          ACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCG                                                                                                             <  SRR19973071.4078746/150‑1 (MQ=60)
                                                     GCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCC                                                                                                  <  SRR19973071.1908059/150‑1 (MQ=60)
                                                     GCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCC                                                                                                  <  SRR19973071.1150035/150‑1 (MQ=60)
                                                      CGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCG                                                                                                 <  SRR19973071.2886714/150‑1 (MQ=60)
                                                      CGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCG                                                                                                 <  SRR19973071.1823973/150‑1 (MQ=60)
                                                        TGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAA                                                                                               >  SRR19973071.609488/1‑150 (MQ=60)
                                                         GAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAA                                                                                              >  SRR19973071.4247258/1‑150 (MQ=60)
                                                          AAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAG                                                                                             >  SRR19973071.2103400/1‑150 (MQ=60)
                                                          AAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAG                                                                                             >  SRR19973071.4509463/1‑150 (MQ=60)
                                                          AAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAG                                                                                             <  SRR19973071.1289751/150‑1 (MQ=60)
                                                            AACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCG                                                                                           <  SRR19973071.3597832/150‑1 (MQ=60)
                                                            AACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCG                                                                                           <  SRR19973071.3597798/150‑1 (MQ=60)
                                                            AACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCG                                                                                           <  SRR19973071.2449770/150‑1 (MQ=60)
                                                            AACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCG                                                                                           <  SRR19973071.4285450/150‑1 (MQ=60)
                                                            AACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCG                                                                                           <  SRR19973071.4285481/150‑1 (MQ=60)
                                                             ACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGG                                                                                          <  SRR19973071.564122/150‑1 (MQ=60)
                                                              CGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGT                                                                                         >  SRR19973071.1143291/1‑150 (MQ=60)
                                                              CGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGT                                                                                         >  SRR19973071.1883984/1‑150 (MQ=60)
                                                              CGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGT                                                                                         >  SRR19973071.1883946/1‑150 (MQ=60)
                                                                TCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGA                                                                                       <  SRR19973071.1182772/150‑1 (MQ=60)
                                                                 CCGCAACCTGACGCCGTTTCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACAGCAGTGTAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGAT                                                                                      <  SRR19973071.162660/150‑1 (MQ=60)
                                                                 CCGCAACCTGACGCCGTATCTGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGAT                                                                                      <  SRR19973071.4532778/150‑1 (MQ=60)
                                                                 CCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGAT                                                                                      >  SRR19973071.689684/1‑150 (MQ=60)
                                                                 CCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGAT                                                                                      <  SRR19973071.163017/150‑1 (MQ=60)
                                                                  CGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATT                                                                                     <  SRR19973071.4186350/150‑1 (MQ=60)
                                                                  CGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATT                                                                                     <  SRR19973071.3406780/150‑1 (MQ=60)
                                                                    CAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGA                                                                                   <  SRR19973071.772918/150‑1 (MQ=60)
                                                                    CAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGA                                                                                   <  SRR19973071.1714782/150‑1 (MQ=60)
                                                                    CAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGA                                                                                   <  SRR19973071.334742/150‑1 (MQ=60)
                                                                    CAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGA                                                                                   <  SRR19973071.4112609/150‑1 (MQ=60)
                                                                    CAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGAGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGA                                                                                   <  SRR19973071.2152346/150‑1 (MQ=60)
                                                                       CCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAA                                                                                <  SRR19973071.3642803/150‑1 (MQ=60)
                                                                       CCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAA                                                                                <  SRR19973071.3642794/150‑1 (MQ=60)
                                                                            CGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACG                                                                           <  SRR19973071.654836/150‑1 (MQ=60)
                                                                            CGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACG                                                                           <  SRR19973071.3792091/150‑1 (MQ=60)
                                                                               CGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGC                                                                        <  SRR19973071.1821847/150‑1 (MQ=60)
                                                                               CGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGC                                                                        <  SRR19973071.4221007/150‑1 (MQ=60)
                                                                                  ATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAAT                                                                     <  SRR19973071.2509691/150‑1 (MQ=60)
                                                                                  ATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAAT                                                                     <  SRR19973071.2509679/150‑1 (MQ=60)
                                                                                   TCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATA                                                                    >  SRR19973071.1039377/1‑150 (MQ=60)
                                                                                    CAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATAT                                                                   <  SRR19973071.3228980/150‑1 (MQ=60)
                                                                                     AGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATG                                                                  >  SRR19973071.2085314/1‑150 (MQ=60)
                                                                                        CGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAG                                                               <  SRR19973071.1609410/150‑1 (MQ=60)
                                                                                        CGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAG                                                               >  SRR19973071.2225939/1‑150 (MQ=60)
                                                                                         GGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGTAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGG                                                              <  SRR19973071.2404551/150‑1 (MQ=60)
                                                                                         GGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGG                                                              <  SRR19973071.1791624/150‑1 (MQ=60)
                                                                                           CGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCG                                                            <  SRR19973071.319431/150‑1 (MQ=60)
                                                                                            GCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGT                                                           <  SRR19973071.3137390/150‑1 (MQ=60)
                                                                                             CGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTA                                                          >  SRR19973071.3379453/1‑150 (MQ=60)
                                                                                              GTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAA                                                         >  SRR19973071.953798/1‑150 (MQ=60)
                                                                                              GTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAA                                                         >  SRR19973071.3753985/1‑150 (MQ=60)
                                                                                              GTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAA                                                         >  SRR19973071.4495443/1‑150 (MQ=60)
                                                                                                  TCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACC                                                     <  SRR19973071.1690461/150‑1 (MQ=60)
                                                                                                      GGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAG                                                 <  SRR19973071.610419/150‑1 (MQ=60)
                                                                                                       GCGGTAACGGCGATGTCTGGCTGAACGCCAACTAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGC                                                <  SRR19973071.1634969/150‑1 (MQ=60)
                                                                                                       GCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGGGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGC                                                >  SRR19973071.784532/1‑150 (MQ=60)
                                                                                                         GGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAG                                              <  SRR19973071.3450807/150‑1 (MQ=60)
                                                                                                         GGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAG                                              <  SRR19973071.3997430/150‑1 (MQ=60)
                                                                                                          GTAAGGGCGTTGTCTGGCTTAACGCAAACGAATACCCCACTGAAGTGTTGTTTAAGCTTACTCAGAAAACGATC‑ACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGT                                            <  SRR19973071.3834270/150‑1 (MQ=60)
                                                                                                          GTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGG                                             <  SRR19973071.1542069/150‑1 (MQ=60)
                                                                                                            AACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTG                                           >  SRR19973071.595425/1‑150 (MQ=60)
                                                                                                            AACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTAAGCGCAATATGCAGGCGTAAAACCGGAGCAGGTG                                           >  SRR19973071.595495/1‑150 (MQ=60)
                                                                                                            AACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTG                                           >  SRR19973071.2287409/1‑150 (MQ=60)
                                                                                                             ACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGC                                          >  SRR19973071.626217/1‑150 (MQ=60)
                                                                                                               GGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCACTATGCAGGCGTAAAACCGGAGCAGGTGCTG                                        >  SRR19973071.1856918/1‑150 (MQ=60)
                                                                                                               GGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTG                                        <  SRR19973071.1867444/150‑1 (MQ=60)
                                                                                                                  GATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTC                                     >  SRR19973071.2230270/1‑150 (MQ=60)
                                                                                                                  GATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTC                                     >  SRR19973071.4636448/1‑150 (MQ=60)
                                                                                                                       CTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCG                                >  SRR19973071.2356085/1‑150 (MQ=60)
                                                                                                                           CTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGC                            <  SRR19973071.2129419/150‑1 (MQ=60)
                                                                                                                            TGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCG                           >  SRR19973071.2213776/1‑150 (MQ=60)
                                                                                                                              AACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCG                         <  SRR19973071.446061/150‑1 (MQ=60)
                                                                                                                              AACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCG                         >  SRR19973071.436822/1‑150 (MQ=60)
                                                                                                                              AACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCG                         >  SRR19973071.794353/1‑150 (MQ=60)
                                                                                                                                 GCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGAC                      >  SRR19973071.2568797/1‑150 (MQ=60)
                                                                                                                                 GCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGAC                      <  SRR19973071.752298/150‑1 (MQ=60)
                                                                                                                                 GCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGAC                      <  SRR19973071.752216/150‑1 (MQ=60)
                                                                                                                                  CCAACGTATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACG                     <  SRR19973071.4419126/150‑1 (MQ=60)
                                                                                                                                  CCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACG                     >  SRR19973071.4577334/1‑150 (MQ=60)
                                                                                                                                    AACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAA                   >  SRR19973071.78241/1‑150 (MQ=60)
                                                                                                                                       GAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGT                <  SRR19973071.1776007/150‑1 (MQ=60)
                                                                                                                                         ATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTAT              <  SRR19973071.543442/150‑1 (MQ=60)
                                                                                                                                         ATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTAT              >  SRR19973071.897758/1‑150 (MQ=60)
                                                                                                                                           ACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTG            >  SRR19973071.3737112/1‑150 (MQ=60)
                                                                                                                                           ACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTG            <  SRR19973071.664386/150‑1 (MQ=60)
                                                                                                                                            CCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGA           >  SRR19973071.118950/1‑150 (MQ=60)
                                                                                                                                              CCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAAC         <  SRR19973071.3459499/150‑1 (MQ=60)
                                                                                                                                              CCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAAC         <  SRR19973071.4458377/150‑1 (MQ=60)
                                                                                                                                              CCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAAC         <  SRR19973071.4088227/150‑1 (MQ=60)
                                                                                                                                              CCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAAC         <  SRR19973071.3459479/150‑1 (MQ=60)
                                                                                                                                               CACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACT        <  SRR19973071.1967420/150‑1 (MQ=60)
                                                                                                                                                     AGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTGCTGAT  <  SRR19973071.803716/150‑1 (MQ=60)
                                                                                                                                                     AGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTGCTGAT  <  SRR19973071.3583355/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TGTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTGCTGAT  >  CP009273/2085855‑2086153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑