Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,086,004 C→A 100% A42A (GCC→GCA)  hisC → histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,086,0040CA99.4% 549.0 / ‑5.6 170A42A (GCC→GCA) hisChistidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (75/93);  total (76/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 170 total aligned reads displayed.

ACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTG  >  CP009273/2085860‑2086148
                                                                                                                                                |                                                                                                                                                
 cGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTgg                                                                                                                                              <  2:1755354/148‑1 (MQ=255)
   cccTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTCGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAg                                                                                                                                            <  2:3225152/148‑1 (MQ=255)
    ccTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTCGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGt                                                                                                                                           <  1:630952/148‑1 (MQ=255)
    ccTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGt                                                                                                                                           <  1:2920492/148‑1 (MQ=255)
    ccTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGt                                                                                                                                           <  2:2926146/148‑1 (MQ=255)
      ttAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGttt                                                                                                                                         >  1:3270742/1‑148 (MQ=255)
      ttAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGttt                                                                                                                                         <  1:617409/148‑1 (MQ=255)
          ggAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGc                                                                                                                                     <  1:1803932/148‑1 (MQ=255)
          ggAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGc                                                                                                                                     >  2:3703524/1‑148 (MQ=255)
          ggAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGc                                                                                                                                     <  2:48157/148‑1 (MQ=255)
          ggAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGc                                                                                                                                     >  2:104375/1‑148 (MQ=255)
           gAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCt                                                                                                                                    >  1:477685/1‑148 (MQ=255)
             gcaagcaTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTa                                                                                                                                  <  1:1832813/148‑1 (MQ=255)
                 gcaTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCa                                                                                                                              >  2:1745644/1‑148 (MQ=255)
                  caTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGACTACCCCACTGCAGTGGAGTTTCAGCTTACTCAg                                                                                                                             >  2:3166704/1‑148 (MQ=255)
                     gAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCaa                                                                                                                          >  1:343503/1‑148 (MQ=255)
                     gAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCaa                                                                                                                          >  1:343578/1‑148 (MQ=255)
                      aGCACCGTGACTATTACCGCTTTAGCGCGTTAAAACGTCCGCAACCAGACGCCGTATCAGTCGGCGAGTCGTCTGGGAGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCaaa                                                                                                                         <  2:2283740/148‑1 (MQ=255)
                       gCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAAc                                                                                                                        >  2:1643613/1‑148 (MQ=255)
                          ccGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCt                                                                                                                     <  1:774885/148‑1 (MQ=255)
                          ccGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCt                                                                                                                     >  2:523453/1‑148 (MQ=255)
                             tGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCaa                                                                                                                  <  1:1001588/148‑1 (MQ=255)
                              gACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAAc                                                                                                                 >  2:1257563/1‑148 (MQ=255)
                              gACTATGACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAAc                                                                                                                 <  2:2118642/148‑1 (MQ=255)
                                cTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCg                                                                                                               <  2:3056750/148‑1 (MQ=255)
                                cTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCg                                                                                                               <  2:3387354/148‑1 (MQ=255)
                                cTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCg                                                                                                               <  2:3387260/148‑1 (MQ=255)
                                 tATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGc                                                                                                              >  2:1350813/1‑148 (MQ=255)
                                   ttACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTa                                                                                                            <  2:1709662/148‑1 (MQ=255)
                                   ttACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTa                                                                                                            >  2:2719191/1‑148 (MQ=255)
                                        gATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCgg                                                                                                       >  2:3025098/1‑148 (MQ=255)
                                        gATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCgg                                                                                                       >  2:1088602/1‑148 (MQ=255)
                                           ttAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGTGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAAt                                                                                                    >  2:2349366/1‑148 (MQ=255)
                                            tAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATg                                                                                                   <  2:3592086/148‑1 (MQ=255)
                                            tAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATg                                                                                                   <  2:3094438/148‑1 (MQ=255)
                                             aGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGc                                                                                                  <  2:678133/148‑1 (MQ=255)
                                              gcgcGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGcc                                                                                                 >  1:292002/1‑148 (MQ=255)
                                              gcgcGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGcc                                                                                                 >  1:1595179/1‑148 (MQ=255)
                                              gcgcGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGcc                                                                                                 >  1:2515725/1‑148 (MQ=255)
                                                gcgTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAg                                                                                               <  1:134403/148‑1 (MQ=255)
                                                    gAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGa                                                                                           >  2:2636923/1‑148 (MQ=255)
                                                     aaaaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGaa                                                                                          <  2:562070/148‑1 (MQ=255)
                                                       aaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAg                                                                                        >  1:1973441/1‑148 (MQ=255)
                                                       aaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAg                                                                                        >  1:1971158/1‑148 (MQ=255)
                                                        aCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGc                                                                                       >  1:428113/1‑148 (MQ=255)
                                                          gTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCgg                                                                                     <  1:1245861/148‑1 (MQ=255)
                                                          gTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCgg                                                                                     <  1:1741674/148‑1 (MQ=255)
                                                          gTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCgg                                                                                     <  1:2244138/148‑1 (MQ=255)
                                                           tCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGt                                                                                    <  1:2865989/148‑1 (MQ=255)
                                                           tCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGt                                                                                    <  1:2320759/148‑1 (MQ=255)
                                                            ccGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTg                                                                                   <  1:1906927/148‑1 (MQ=255)
                                                            ccGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTg                                                                                   <  1:1257563/148‑1 (MQ=255)
                                                             cGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGa                                                                                  <  1:1819914/148‑1 (MQ=255)
                                                                   cTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGTGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGaaa                                                                            <  2:54454/148‑1 (MQ=255)
                                                                   cTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGaaa                                                                            <  1:1745644/148‑1 (MQ=255)
                                                                   cTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGaaa                                                                            <  2:1986843/148‑1 (MQ=255)
                                                                   cTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGaaa                                                                            <  1:1638068/148‑1 (MQ=255)
                                                                   cTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGaaa                                                                            <  1:1465779/148‑1 (MQ=255)
                                                                     gACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAAt                                                                          <  2:755403/148‑1 (MQ=255)
                                                                      aCGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAAtt                                                                         >  1:2834081/1‑148 (MQ=255)
                                                                      aCGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAAtt                                                                         >  2:150777/1‑148 (MQ=255)
                                                                        gCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTAc                                                                       >  1:2655300/1‑148 (MQ=255)
                                                                             aTCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCa                                                                  >  1:665592/1‑148 (MQ=255)
                                                                             aTCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCa                                                                  <  1:55878/148‑1 (MQ=255)
                                                                             aTCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCa                                                                  <  1:2549752/148‑1 (MQ=255)
                                                                              tCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCaa                                                                 <  1:2030394/148‑1 (MQ=255)
                                                                               cAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAat                                                                <  2:773348/148‑1 (MQ=255)
                                                                               cAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAat                                                                <  1:305604/148‑1 (MQ=255)
                                                                               cAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAat                                                                <  1:305598/148‑1 (MQ=255)
                                                                                    ggCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCa                                                           <  1:1117041/148‑1 (MQ=255)
                                                                                         gtcgtcTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGt                                                      <  1:1775962/148‑1 (MQ=255)
                                                                                            gtcTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTaaa                                                   <  1:186305/148‑1 (MQ=255)
                                                                                             tcTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTaaaa                                                  >  2:828953/1‑148 (MQ=255)
                                                                                                 ggCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCgg                                              >  1:286683/1‑148 (MQ=255)
                                                                                                     gTAACGGCGATGTCTGGCTGAACGCCAACGAGTACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCa                                          <  1:3068551/148‑1 (MQ=255)
                                                                                                      tAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAg                                         >  2:2467743/1‑148 (MQ=255)
                                                                                                      tAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAg                                         >  2:2774767/1‑148 (MQ=255)
                                                                                                      tAACGGCGATGTCTGGCTGAACGCCAACGAAGACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAg                                         <  2:2404538/148‑1 (MQ=255)
                                                                                                            cGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTg                                   >  2:779357/1‑148 (MQ=255)
                                                                                                              aTGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGt                                 >  2:2112854/1‑148 (MQ=255)
                                                                                                                gTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCa                               >  1:998987/1‑148 (MQ=255)
                                                                                                                 tCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAg                              >  1:1997994/1‑148 (MQ=255)
                                                                                                                     gCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGt                          >  1:2455148/1‑148 (MQ=255)
                                                                                                                      cTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCACTATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTg                         <  1:301967/148‑1 (MQ=255)
                                                                                                                      cTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTg                         >  1:161960/1‑148 (MQ=255)
                                                                                                                        gAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGgc                       >  1:3550663/1‑148 (MQ=255)
                                                                                                                         aaCGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGgcg                      >  2:1245624/1‑148 (MQ=255)
                                                                                                                         aaCGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGgcg                      >  2:413916/1‑148 (MQ=255)
                                                                                                                            gCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCgg                   <  1:2137725/148‑1 (MQ=255)
                                                                                                                              cAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGAc                 >  2:3254926/1‑148 (MQ=255)
                                                                                                                                 cGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGTAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGaa              >  1:1282476/1‑148 (MQ=255)
                                                                                                                                  gAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAg             <  1:523453/148‑1 (MQ=255)
                                                                                                                                  gAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAg             <  1:525150/148‑1 (MQ=255)
                                                                                                                                     tACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTa          <  1:2890299/148‑1 (MQ=255)
                                                                                                                                       ccccACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTAtt        >  2:1478496/1‑148 (MQ=255)
                                                                                                                                        cccACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTg       >  1:404373/1‑148 (MQ=255)
                                                                                                                                          cACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGaa     >  2:3478447/1‑148 (MQ=255)
                                                                                                                                           aCTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAAc    <  2:3595707/148‑1 (MQ=255)
                                                                                                                                           aCTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAAc    <  2:2864612/148‑1 (MQ=255)
                                                                                                                                           aCTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAAc    <  2:979791/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTG  >  CP009273/2085860‑2086148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTGCT  >  CP009273/2085856‑2086150
                                                                                                                                                    |                                                                                                                                                  
GTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAG                                                                                                                                                   >  SRR19973069.1789203/1‑150 (MQ=60)
  TAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCTCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTG                                                                                                                                                 >  SRR19973069.982604/1‑150 (MQ=60)
       CCCTTATGGAGCAAGATTGAGCACCGTGAATATTACCTATTTCGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTT                                                                                                                                            <  SRR19973069.124574/150‑1 (MQ=60)
        CCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTCGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTT                                                                                                                                           <  SRR19973069.635121/150‑1 (MQ=60)
        CCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTT                                                                                                                                           <  SRR19973069.2939835/150‑1 (MQ=60)
        CCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTT                                                                                                                                           >  SRR19973069.3292159/1‑150 (MQ=60)
          TTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCA                                                                                                                                         <  SRR19973069.621520/150‑1 (MQ=60)
            AAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGC                                                                                                                                       >  SRR19973069.18527/1‑150 (MQ=60)
             AGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCT                                                                                                                                      >  SRR19973069.481151/1‑150 (MQ=60)
              GGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTT                                                                                                                                     <  SRR19973069.1816624/150‑1 (MQ=60)
              GGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTT                                                                                                                                     <  SRR19973069.1816063/150‑1 (MQ=60)
                 GCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACT                                                                                                                                  <  SRR19973069.1847261/150‑1 (MQ=60)
                 GCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACT                                                                                                                                  <  SRR19973069.1845653/150‑1 (MQ=60)
                       ATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAA                                                                                                                            >  SRR19973069.346008/1‑150 (MQ=60)
                       ATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAA                                                                                                                            >  SRR19973069.345932/1‑150 (MQ=60)
                          AGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTAAGCTTACTCAGacaaag                                                                                                                         >  SRR19973069.2921809/1‑144 (MQ=60)
                              CCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCA                                                                                                                     <  SRR19973069.1993326/150‑1 (MQ=60)
                              CCGTGAATATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCA                                                                                                                     <  SRR19973069.780373/150‑1 (MQ=60)
                               CGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAA                                                                                                                    >  SRR19973069.1399522/1‑150 (MQ=60)
                                 TGAATATTACGGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACC                                                                                                                  <  SRR19973069.1008454/150‑1 (MQ=60)
                                  GACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCG                                                                                                                 >  SRR19973069.778825/1‑150 (MQ=60)
                                                TAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCC                                                                                                   >  SRR19973069.1605949/1‑150 (MQ=60)
                                                TAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCC                                                                                                   >  SRR19973069.602934/1‑150 (MQ=60)
                                                TAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCC                                                                                                   >  SRR19973069.2532470/1‑150 (MQ=60)
                                                TAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCC                                                                                                   >  SRR19973069.294011/1‑150 (MQ=60)
                                                  GCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAG                                                                                                 <  SRR19973069.2853621/150‑1 (MQ=60)
                                                    GCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCC                                                                                               <  SRR19973069.135654/150‑1 (MQ=60)
                                                         AAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAG                                                                                          >  SRR19973069.1984841/1‑150 (MQ=60)
                                                         AAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAG                                                                                          >  SRR19973069.1984852/1‑150 (MQ=60)
                                                         AAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAG                                                                                          >  SRR19973069.1987155/1‑150 (MQ=60)
                                                          AAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGC                                                                                         >  SRR19973069.393485/1‑150 (MQ=60)
                                                          AAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGC                                                                                         >  SRR19973069.431258/1‑150 (MQ=60)
                                                              GTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTG                                                                                     >  SRR19973069.1779689/1‑150 (MQ=60)
                                                              GTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTG                                                                                     <  SRR19973069.2259255/150‑1 (MQ=60)
                                                              GTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTG                                                                                     <  SRR19973069.1254315/150‑1 (MQ=60)
                                                              GTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTG                                                                                     <  SRR19973069.1753896/150‑1 (MQ=60)
                                                               TCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGA                                                                                    <  SRR19973069.2884822/150‑1 (MQ=60)
                                                               TCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGA                                                                                    <  SRR19973069.1647273/150‑1 (MQ=60)
                                                               TCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGA                                                                                    <  SRR19973069.2336545/150‑1 (MQ=60)
                                                                CCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGAT                                                                                   <  SRR19973069.1920096/150‑1 (MQ=60)
                                                                CCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGAT                                                                                   <  SRR19973069.1266053/150‑1 (MQ=60)
                                                                CCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGAT                                                                                   <  SRR19973069.3077736/150‑1 (MQ=60)
                                                                 CGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATT                                                                                  <  SRR19973069.1832691/150‑1 (MQ=60)
                                                                       CTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAAT                                                                            <  SRR19973069.1476008/150‑1 (MQ=60)
                                                                       CTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAAT                                                                            <  SRR19973069.1649304/150‑1 (MQ=60)
                                                                       CTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAAT                                                                            <  SRR19973069.1757912/150‑1 (MQ=60)
                                                                       CTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAAT                                                                            <  SRR19973069.3555427/150‑1 (MQ=60)
                                                                        TGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATT                                                                           >  SRR19973069.2852792/1‑150 (MQ=60)
                                                                          ACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTAC                                                                         >  SRR19973069.2673091/1‑150 (MQ=60)
                                                                          ACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTAC                                                                         >  SRR19973069.2673096/1‑150 (MQ=60)
                                                                               GTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCA                                                                    >  SRR19973069.670120/1‑150 (MQ=60)
                                                                                 ATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAAT                                                                  <  SRR19973069.2566598/150‑1 (MQ=60)
                                                                                 ATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAAT                                                                  <  SRR19973069.56562/150‑1 (MQ=60)
                                                                                  TCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATA                                                                 <  SRR19973069.2044500/150‑1 (MQ=60)
                                                                                   CAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATAT                                                                <  SRR19973069.307657/150‑1 (MQ=60)
                                                                                   CAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATAT                                                                <  SRR19973069.307663/150‑1 (MQ=60)
                                                                                       CGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAG                                                            <  SRR19973069.3328766/150‑1 (MQ=60)
                                                                                        GGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGG                                                           <  SRR19973069.1124833/150‑1 (MQ=60)
                                                                                             GTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAA                                                      <  SRR19973069.1788470/150‑1 (MQ=60)
                                                                                                GTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAAC                                                   <  SRR19973069.1262570/150‑1 (MQ=60)
                                                                                                GTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAAC                                                   <  SRR19973069.187842/150‑1 (MQ=60)
                                                                                                 TCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACC                                                  <  SRR19973069.3005847/150‑1 (MQ=60)
                                                                                                 TCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACC                                                  <  SRR19973069.1938403/150‑1 (MQ=60)
                                                                                                   TGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGG                                                >  SRR19973069.288668/1‑150 (MQ=60)
                                                                                                   TGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGG                                                >  SRR19973069.288663/1‑150 (MQ=60)
                                                                                                        GGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAG                                           >  SRR19973069.2061184/1‑150 (MQ=60)
                                                                                                         GTAACGGCGATGTCTGGCTGAACGCCAACGAGTACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGG                                          <  SRR19973069.3088828/150‑1 (MQ=60)
                                                                                                         GTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGG                                          <  SRR19973069.3089347/150‑1 (MQ=60)
                                                                                                           AACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTG                                        >  SRR19973069.2516033/1‑150 (MQ=60)
                                                                                                                CGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGT                                   >  SRR19973069.2257271/1‑150 (MQ=60)
                                                                                                                  ATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCA                                 >  SRR19973069.1005826/1‑150 (MQ=60)
                                                                                                                   TGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAG                                >  SRR19973069.2011913/1‑150 (MQ=60)
                                                                                                                       TGGCTGAACGCCAACGAATACCCCACTGCAGTGTAGTTTCAGCTTACTCAGAAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAATCGGGGATTGAAAATTACGCGCAATATGCAGGCGTAAAAACGGAGCAGGTGatgttcatcaga                            >  SRR19973069.539493/1‑138 (MQ=60)
                                                                                                                       TGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGT                            >  SRR19973069.2471652/1‑150 (MQ=60)
                                                                                                                        GGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTG                           >  SRR19973069.163365/1‑150 (MQ=60)
                                                                                                                        GGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTG                           >  SRR19973069.162913/1‑150 (MQ=60)
                                                                                                                          CTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCACTATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGC                         <  SRR19973069.304011/150‑1 (MQ=60)
                                                                                                                          CTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGC                         >  SRR19973069.167987/1‑150 (MQ=60)
                                                                                                                          CTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGC                         <  SRR19973069.304686/150‑1 (MQ=60)
                                                                                                                          CNGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGC                         >  SRR19973069.3573740/1‑150 (MQ=60)
                                                                                                                                GCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGAC                   <  SRR19973069.2152407/150‑1 (MQ=60)
                                                                                                                                   AACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGTAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAA                >  SRR19973069.1291485/1‑150 (MQ=60)
                                                                                                                                   AACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGTAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAA                >  SRR19973069.1291063/1‑150 (MQ=60)
                                                                                                                                      GAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGT             <  SRR19973069.528869/150‑1 (MQ=60)
                                                                                                                                      GAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGT             <  SRR19973069.527163/150‑1 (MQ=60)
                                                                                                                                         TACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATT          <  SRR19973069.2909347/150‑1 (MQ=60)
                                                                                                                                          ACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTG         <  SRR19973069.626093/150‑1 (MQ=60)
                                                                                                                                          ACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTG         >  SRR19973069.407335/1‑150 (MQ=60)
                                                                                                                                              CACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACT     <  SRR19973069.2737412/150‑1 (MQ=60)
                                                                                                                                               ACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTG    <  SRR19973069.465214/150‑1 (MQ=60)
                                                                                                                                                 TGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTGCT  <  SRR19973069.2365347/150‑1 (MQ=60)
                                                                                                                                                 TGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTGCT  <  SRR19973069.521930/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
GTTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTGCT  >  CP009273/2085856‑2086150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑