Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,101,912 T→G 93.3% E385A (GAG→GCG)  wzxB ← putative polisoprenol‑linked O‑antigen transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,101,9120TG93.3% 728.6 / 16.2 199E385A (GAG→GCG) wzxBputative polisoprenol‑linked O‑antigen transporter
Reads supporting (aligned to +/- strand):  ref base T (6/7);  new base G (87/98);  total (93/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 202 total aligned reads displayed.

AACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCA  >  CP009273/2101766‑2102056
                                                                                                                                                  |                                                                                                                                                
aaCGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAAtctc                                                                                                                                                 <  1:1360147/148‑1 (MQ=255)
 aCGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATcgct                                                                                                                                                >  2:679309/1‑148 (MQ=255)
  cGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCtt                                                                                                                                               >  2:1776535/1‑148 (MQ=255)
         aaacaaacCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAag                                                                                                                                        >  1:1036950/1‑148 (MQ=255)
              aacCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAgttg                                                                                                                                   <  1:1476386/148‑1 (MQ=255)
              aacCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAgttg                                                                                                                                   >  2:3196574/1‑148 (MQ=255)
               acCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGTTGCACCAATCGCTTTAAAAAGAgttgt                                                                                                                                  <  2:2607042/148‑1 (MQ=255)
               acCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAgttgt                                                                                                                                  <  1:801578/148‑1 (MQ=255)
               acCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAgttgt                                                                                                                                  <  2:1361947/148‑1 (MQ=255)
               acCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAgttgt                                                                                                                                  <  2:2967790/148‑1 (MQ=255)
                ccAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAgttgtt                                                                                                                                 <  2:1476393/148‑1 (MQ=255)
                ccAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAgttgtt                                                                                                                                 >  2:910735/1‑148 (MQ=255)
                ccAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAgttgtt                                                                                                                                 <  2:2482418/148‑1 (MQ=255)
                 cAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTa                                                                                                                                <  1:1761422/148‑1 (MQ=255)
                 cAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTa                                                                                                                                <  1:3104804/148‑1 (MQ=255)
                   gAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGc                                                                                                                              >  2:572163/1‑148 (MQ=255)
                     aCCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCgg                                                                                                                            <  1:3042787/148‑1 (MQ=255)
                        aacaaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGaaa                                                                                                                         >  2:1038758/1‑148 (MQ=255)
                         acaaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGaaaa                                                                                                                        <  1:3264691/148‑1 (MQ=255)
                          caaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGaaaaa                                                                                                                       <  1:2602668/148‑1 (MQ=255)
                           aaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAAt                                                                                                                      >  1:2652976/1‑148 (MQ=255)
                            aTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATc                                                                                                                     >  1:2964230/1‑148 (MQ=255)
                             tGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATca                                                                                                                    >  2:1926244/1‑148 (MQ=255)
                              gATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATcaa                                                                                                                   <  1:653998/148‑1 (MQ=255)
                                tataATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATcaaca                                                                                                                 <  2:2215914/148‑1 (MQ=255)
                                tataATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATcaaca                                                                                                                 <  2:2743013/148‑1 (MQ=255)
                                 ataATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATcaacaa                                                                                                                >  1:802835/1‑148 (MQ=255)
                                        tACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTc                                                                                                         >  1:1515175/1‑148 (MQ=255)
                                         aCATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTca                                                                                                        <  2:1279827/148‑1 (MQ=255)
                                            tAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTcaaca                                                                                                     <  1:3079234/148‑1 (MQ=255)
                                             aaaaTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTcaacaa                                                                                                    <  1:1981876/148‑1 (MQ=255)
                                             aaaaTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTcaacaa                                                                                                    >  2:2601073/1‑148 (MQ=255)
                                              aaaTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACaaa                                                                                                   >  2:1467232/1‑148 (MQ=255)
                                                aTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAcc                                                                                                 >  1:397877/1‑148 (MQ=255)
                                                 tCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAccc                                                                                                >  2:798383/1‑148 (MQ=255)
                                                 tCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAccc                                                                                                >  2:1562139/1‑148 (MQ=255)
                                                   cTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGc                                                                                              <  2:2012128/148‑1 (MQ=255)
                                                       gCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCg                                                                                          <  2:2979402/148‑1 (MQ=255)
                                                       gCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCg                                                                                          >  2:1234107/1‑148 (MQ=255)
                                                          caCCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGAtt                                                                                       <  2:2283730/147‑1 (MQ=255)
                                                          aaCCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGAtt                                                                                       >  2:1850610/1‑148 (MQ=255)
                                                             cAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTaaa                                                                                    <  2:1288530/148‑1 (MQ=255)
                                                              aGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTaaaa                                                                                   >  2:2540191/1‑148 (MQ=255)
                                                                tAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATc                                                                                 <  2:649826/148‑1 (MQ=255)
                                                                tAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATc                                                                                 <  1:268385/148‑1 (MQ=255)
                                                                 aaTTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCt                                                                                >  1:2520415/1‑148 (MQ=255)
                                                                  aTTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCtt                                                                               <  1:679309/148‑1 (MQ=255)
                                                                   tttATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTa                                                                              <  2:325641/148‑1 (MQ=255)
                                                                    ttattaTTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTAc                                                                             >  2:2627245/1‑148 (MQ=255)
                                                                      attattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTg                                                                           >  2:46958/1‑148 (MQ=255)
                                                                        tattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGaa                                                                         <  2:542385/148‑1 (MQ=255)
                                                                        tattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGaa                                                                         >  2:97438/1‑148 (MQ=255)
                                                                          tttCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAtt                                                                       <  1:800499/148‑1 (MQ=255)
                                                                               tACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAttctttc                                                                  <  1:2014245/148‑1 (MQ=255)
                                                                               tACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAttctttc                                                                  >  2:2714578/1‑148 (MQ=255)
                                                                                 cGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATtctttctt                                                                <  2:1147392/148‑1 (MQ=255)
                                                                                  gAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTta                                                               <  2:3311884/148‑1 (MQ=255)
                                                                                   aaCATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTtat                                                              <  1:216967/148‑1 (MQ=255)
                                                                                       tCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAAtt                                                          <  1:1776535/148‑1 (MQ=255)
                                                                                       tCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAAtt                                                          >  2:1567258/1‑148 (MQ=255)
                                                                                        cAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTa                                                         <  1:1926252/148‑1 (MQ=255)
                                                                                         aGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGGGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTAt                                                        >  2:2829986/1‑148 (MQ=255)
                                                                                         aGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTAt                                                        <  1:3287592/148‑1 (MQ=255)
                                                                                           cATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATgg                                                      >  2:1001132/1‑148 (MQ=255)
                                                                                           cATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATgg                                                      <  1:2785561/148‑1 (MQ=255)
                                                                                           cATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATgg                                                      >  2:3230690/1‑148 (MQ=255)
                                                                                             tGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGt                                                    <  2:1723971/148‑1 (MQ=255)
                                                                                             tGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGt                                                    <  2:1511464/148‑1 (MQ=255)
                                                                                                 tGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGc                                                <  1:1038758/148‑1 (MQ=255)
                                                                                                  gACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCa                                               >  2:1376022/1‑148 (MQ=255)
                                                                                                  gACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCa                                               <  1:2253049/148‑1 (MQ=255)
                                                                                                       aaCTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACt                                          >  2:2285867/1‑148 (MQ=255)
                                                                                                         cTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTg                                        >  1:2490126/1‑148 (MQ=255)
                                                                                                          tAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGa                                       <  2:1103252/148‑1 (MQ=255)
                                                                                                           aaGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGaa                                      >  2:42811/1‑148 (MQ=255)
                                                                                                           aaGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGaa                                      >  1:231468/1‑148 (MQ=255)
                                                                                                           aaGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGaa                                      >  1:1900695/1‑148 (MQ=255)
                                                                                                            aGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAAt                                     >  2:1690000/1‑148 (MQ=255)
                                                                                                            aGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAAt                                     >  2:1689079/1‑148 (MQ=255)
                                                                                                             gCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATg                                    >  2:1737555/1‑148 (MQ=255)
                                                                                                             gCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATg                                    >  1:2134355/1‑148 (MQ=255)
                                                                                                              cACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGc                                   >  1:1827915/1‑148 (MQ=255)
                                                                                                              cACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGc                                   <  1:3028994/148‑1 (MQ=255)
                                                                                                              cACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGc                                   <  1:1447341/148‑1 (MQ=255)
                                                                                                               aCTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGcc                                  <  1:572173/148‑1 (MQ=255)
                                                                                                                 tctGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCAt                                <  1:516766/148‑1 (MQ=255)
                                                                                                                    gTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGa                             >  2:3060180/1‑148 (MQ=255)
                                                                                                                     ttGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGAc                            <  1:2925351/148‑1 (MQ=255)
                                                                                                                       gcaagcaaTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACATCAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACAt                          <  1:1273766/148‑1 (MQ=255)
                                                                                                                         aagcaaTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTa                        <  1:549405/148‑1 (MQ=255)
                                                                                                                             aaTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTa                    <  2:1105142/148‑1 (MQ=255)
                                                                                                                              aTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTaa                   <  1:3213490/148‑1 (MQ=255)
                                                                                                                                gTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATg                 >  1:911590/1‑148 (MQ=255)
                                                                                                                                 ttATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGa                <  2:1478346/148‑1 (MQ=255)
                                                                                                                                   aTTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGaaa              <  2:1036950/148‑1 (MQ=255)
                                                                                                                                   aTTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGaaa              <  1:1234107/148‑1 (MQ=255)
                                                                                                                                     tgctgcACCAATCACTTAAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATa            <  2:1999395/148‑1 (MQ=255)
                                                                                                                                      gctgcACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATaa           >  2:279216/1‑148 (MQ=255)
                                                                                                                                           aCCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGaaaa      <  2:2204193/148‑1 (MQ=255)
                                                                                                                                              aaTCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGc   >  2:612166/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
AACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCA  >  CP009273/2101766‑2102056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAG  >  CP009273/2101765‑2102059
                                                                                                                                                   |                                                                                                                                                   
AAACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCT                                                                                                                                                   <  SRR19973070.213525/150‑1 (MQ=60)
AAACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCT                                                                                                                                                   <  SRR19973070.216045/150‑1 (MQ=60)
 AACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTT                                                                                                                                                  <  SRR19973070.1369423/150‑1 (MQ=60)
        CCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAG                                                                                                                                           >  SRR19973070.1044277/1‑150 (MQ=60)
               AACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTT                                                                                                                                    <  SRR19973070.1486713/150‑1 (MQ=60)
                ACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTTACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTA                                                                                                                                   <  SRR19973070.1407431/150‑1 (MQ=60)
                ACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTA                                                                                                                                   <  SRR19973070.807100/150‑1 (MQ=60)
                 CCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAG                                                                                                                                  >  SRR19973070.3054714/1‑150 (MQ=60)
                 CCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAG                                                                                                                                  <  SRR19973070.2713199/150‑1 (MQ=60)
                  CAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGC                                                                                                                                 <  SRR19973070.3124828/150‑1 (MQ=60)
                  CAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGC                                                                                                                                 <  SRR19973070.1773613/150‑1 (MQ=60)
                      ACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAA                                                                                                                             <  SRR19973070.3062127/150‑1 (MQ=60)
                          ACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAAT                                                                                                                         <  SRR19973070.3285702/150‑1 (MQ=60)
                          ACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAAT                                                                                                                         >  SRR19973070.2670238/1‑150 (MQ=60)
                          ACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAAT                                                                                                                         >  SRR19973070.2669715/1‑150 (MQ=60)
                           CAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATC                                                                                                                        <  SRR19973070.1243926/150‑1 (MQ=60)
                           CAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATC                                                                                                                        >  SRR19973070.1506608/1‑150 (MQ=60)
                           CAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATC                                                                                                                        >  SRR19973070.2983288/1‑150 (MQ=60)
                           CAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATC                                                                                                                        <  SRR19973070.2619659/150‑1 (MQ=60)
                           CAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATC                                                                                                                        <  SRR19973070.2059498/150‑1 (MQ=60)
                            AATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCA                                                                                                                       <  SRR19973070.3286445/150‑1 (MQ=60)
                               GATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACA                                                                                                                    <  SRR19973070.658662/150‑1 (MQ=60)
                                ATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAA                                                                                                                   >  SRR19973070.808361/1‑150 (MQ=60)
                                  ATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACA                                                                                                                 >  SRR19973070.1812466/1‑150 (MQ=60)
                                       CGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTC                                                                                                            >  SRR19973070.961885/1‑150 (MQ=60)
                                       CGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTC                                                                                                            >  SRR19973070.1525790/1‑150 (MQ=60)
                                       CGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTC                                                                                                            <  SRR19973070.1337568/150‑1 (MQ=60)
                                           CATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACA                                                                                                        >  SRR19973070.1886584/1‑150 (MQ=60)
                                             TAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAA                                                                                                      <  SRR19973070.3099019/150‑1 (MQ=60)
                                              AAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAC                                                                                                     <  SRR19973070.1995286/150‑1 (MQ=60)
                                               AAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACC                                                                                                    >  SRR19973070.400804/1‑150 (MQ=60)
                                                          AAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTA                                                                                         <  SRR19973070.1959000/150‑1 (MQ=60)
                                                               AGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATC                                                                                    <  SRR19973070.2596695/150‑1 (MQ=60)
                                                                gactggagttcagacgtgtgctcttccgatctAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCT                                                                                   <  SRR19973070.545032/118‑1 (MQ=60)
                                                                GTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCT                                                                                   >  SRR19973070.2536848/1‑150 (MQ=60)
                                                                 TAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTT                                                                                  <  SRR19973070.270152/150‑1 (MQ=60)
                                                                   ATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTAC                                                                                <  SRR19973070.3240263/150‑1 (MQ=60)
                                                                   ATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTAC                                                                                <  SRR19973070.684151/150‑1 (MQ=60)
                                                                           TTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCT                                                                        <  SRR19973070.806018/150‑1 (MQ=60)
                                                                           TTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCT                                                                        <  SRR19973070.806025/150‑1 (MQ=60)
                                                                            TTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTT                                                                       <  SRR19973070.1640996/150‑1 (MQ=60)
                                                                              CTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTC                                                                     >  SRR19973070.679882/1‑150 (MQ=60)
                                                                                TACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTT                                                                   <  SRR19973070.2027868/150‑1 (MQ=60)
                                                                                  CGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTAT                                                                 <  SRR19973070.74445/150‑1 (MQ=60)
                                                                                  CGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTAT                                                                 <  SRR19973070.2231452/150‑1 (MQ=60)
                                                                                   GAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATA                                                                >  SRR19973070.2863247/1‑150 (MQ=60)
                                                                                    AACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAA                                                               <  SRR19973070.218547/150‑1 (MQ=60)
                                                                                      CATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATT                                                             <  SRR19973070.2690443/150‑1 (MQ=60)
                                                                                        TCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTAT                                                           <  SRR19973070.1788829/150‑1 (MQ=60)
                                                                                         CAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATTTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATG                                                          <  SRR19973070.1939234/150‑1 (MQ=60)
                                                                                         CAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATG                                                          <  SRR19973070.1939225/150‑1 (MQ=60)
                                                                                          AGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGG                                                         >  SRR19973070.3080787/1‑150 (MQ=60)
                                                                                          AGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGG                                                         <  SRR19973070.3308694/150‑1 (MQ=60)
                                                                                          AGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGG                                                         <  SRR19973070.3308812/150‑1 (MQ=60)
                                                                                            CATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGT                                                       <  SRR19973070.2803483/150‑1 (MQ=60)
                                                                                             ATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTC                                                      <  SRR19973070.591271/150‑1 (MQ=60)
                                                                                                AGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGC                                                   >  SRR19973070.2442107/1‑150 (MQ=60)
                                                                                                  TGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCAT                                                 <  SRR19973070.1046092/150‑1 (MQ=60)
                                                                                                  TGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCAT                                                 <  SRR19973070.1046041/150‑1 (MQ=60)
                                                                                                   GACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATA                                                <  SRR19973070.2267978/150‑1 (MQ=60)
                                                                                                   GACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATA                                                <  SRR19973070.1788534/150‑1 (MQ=60)
                                                                                                        AACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTG                                           <  SRR19973070.1468906/150‑1 (MQ=60)
                                                                                                        AACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTG                                           >  SRR19973070.2506291/1‑150 (MQ=60)
                                                                                                        AACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTG                                           >  SRR19973070.2506418/1‑150 (MQ=60)
                                                                                                          CTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAA                                         >  SRR19973070.1913561/1‑150 (MQ=60)
                                                                                                          CTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAA                                         >  SRR19973070.233101/1‑150 (MQ=60)
                                                                                                            AAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATG                                       >  SRR19973070.2148615/1‑150 (MQ=60)
                                                                                                             AGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGC                                      >  SRR19973070.1840485/1‑150 (MQ=60)
                                                                                                               CACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCA                                    <  SRR19973070.1457400/150‑1 (MQ=60)
                                                                                                               CACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCA                                    <  SRR19973070.3048276/150‑1 (MQ=60)
                                                                                                                TCTCTGTTGCAATCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGTAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCAT                                   <  SRR19973070.576062/150‑1 (MQ=60)
                                                                                                                ACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCAT                                   >  SRR19973070.299933/1‑150 (MQ=60)
                                                                                                                ACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCAT                                   <  SRR19973070.2301003/150‑1 (MQ=60)
                                                                                                                ACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCAT                                   <  SRR19973070.576072/150‑1 (MQ=60)
                                                                                                                  TCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAG                                 <  SRR19973070.520300/150‑1 (MQ=60)
                                                                                                                      TTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACAT                             <  SRR19973070.2944237/150‑1 (MQ=60)
                                                                                                                        GCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACATCAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTA                           <  SRR19973070.1282676/150‑1 (MQ=60)
                                                                                                                        GCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTA                           <  SRR19973070.3111991/150‑1 (MQ=60)
                                                                                                                          AAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACT                         <  SRR19973070.553058/150‑1 (MQ=60)
                                                                                                                          AAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACT                         <  SRR19973070.2156049/150‑1 (MQ=60)
                                                                                                                            GCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTA                       >  SRR19973070.81003/1‑150 (MQ=60)
                                                                                                                              AATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAAT                     >  SRR19973070.3155338/1‑150 (MQ=60)
                                                                                                                               ATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATG                    >  SRR19973070.918072/1‑150 (MQ=60)
                                                                                                                               ATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATG                    <  SRR19973070.3234272/150‑1 (MQ=60)
                                                                                                                               ATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATG                    >  SRR19973070.1114483/1‑150 (MQ=60)
                                                                                                                                    ATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATA               <  SRR19973070.1242746/150‑1 (MQ=60)
                                                                                                                                          GCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAA         >  SRR19973070.2983275/1‑150 (MQ=60)
                                                                                                                                          GCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAA         >  SRR19973070.464818/1‑150 (MQ=60)
                                                                                                                                             CCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGC      >  SRR19973070.913284/1‑150 (MQ=60)
                                                                                                                                              CAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCA     >  SRR19973070.202504/1‑150 (MQ=60)
                                                                                                                                                ATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGA   >  SRR19973070.1236891/1‑150 (MQ=60)
                                                                                                                                                 TCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAG  <  SRR19973070.2058911/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                   
AAACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAG  >  CP009273/2101765‑2102059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑