Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,101,912 T→G 87.8% E385A (GAG→GCG)  wzxB ← putative polisoprenol‑linked O‑antigen transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,101,9120TG87.8% 792.6 / 62.1 245E385A (GAG→GCG) wzxBputative polisoprenol‑linked O‑antigen transporter
Reads supporting (aligned to +/- strand):  ref base T (9/21);  new base G (96/119);  total (105/140)
Fisher's exact test for biased strand distribution p-value = 1.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.19e-01

Only 100 of 246 total aligned reads displayed.

CAAACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCA  >  CP009273/2101764‑2102056
                                                                                                                                                    |                                                                                                                                                
   aCGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCtt                                                                                                                                               <  1:3904462/149‑1 (MQ=255)
    cGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCttt                                                                                                                                              >  2:3126729/1‑149 (MQ=255)
           aaacaaacCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAaga                                                                                                                                       >  1:2173774/1‑149 (MQ=255)
            aacacacCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAagag                                                                                                                                      <  2:2289807/149‑1 (MQ=255)
              caaacCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAgtt                                                                                                                                    <  2:3721591/149‑1 (MQ=255)
              caaacCAGAACCAACAATGATATAATAGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTTCTGCACCAATCGCTTTAAAAAGAgtt                                                                                                                                    <  2:3911335/149‑1 (MQ=255)
               aaacCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAgttg                                                                                                                                   <  1:1251540/149‑1 (MQ=255)
                aacCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAgttgt                                                                                                                                  <  2:1792861/149‑1 (MQ=255)
                 acCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAgttgtt                                                                                                                                 >  1:3338721/1‑149 (MQ=255)
                  ccAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTa                                                                                                                                <  2:567026/149‑1 (MQ=255)
                      aaCCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCgg                                                                                                                            <  1:1481803/149‑1 (MQ=255)
                       aCCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTGTAAAAAGAGTTGTTAGCGGa                                                                                                                           >  1:1919110/1‑149 (MQ=255)
                        ccAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGaa                                                                                                                          <  2:2740135/149‑1 (MQ=255)
                        ccAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCAACAATCTCTTTAAAAAGAGTTGTTAGCGGaa                                                                                                                          <  2:2993900/149‑1 (MQ=255)
                          aacaaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGaaaa                                                                                                                        >  1:4594652/1‑149 (MQ=255)
                           acaaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGaaaaa                                                                                                                       <  2:4546835/149‑1 (MQ=255)
                           acaaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGaaaaa                                                                                                                       >  2:1395492/1‑149 (MQ=255)
                            caaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAAt                                                                                                                      <  1:3492029/149‑1 (MQ=255)
                             aaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATc                                                                                                                     <  2:3064133/149‑1 (MQ=255)
                               tGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATcaa                                                                                                                   <  1:699898/149‑1 (MQ=255)
                                 atatAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATcaaca                                                                                                                 >  2:3889845/1‑149 (MQ=255)
                                 atatAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATcaaca                                                                                                                 >  2:826871/1‑149 (MQ=255)
                                  tataATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATcaacaa                                                                                                                <  1:2744887/149‑1 (MQ=255)
                                  tataATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATcaacaa                                                                                                                <  2:2348918/149‑1 (MQ=255)
                                    taATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTGTAAAAAGAGTTGTTAGCGGAAAAATcaacaaca                                                                                                              >  1:4148996/1‑149 (MQ=255)
                                      aTCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACaaa                                                                                                            >  1:4728128/1‑149 (MQ=255)
                                           aCATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTcaa                                                                                                       <  1:931156/149‑1 (MQ=255)
                                              tAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTcaacaa                                                                                                    >  1:4024126/1‑149 (MQ=255)
                                                 aaTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAcc                                                                                                 >  2:1439449/1‑149 (MQ=255)
                                                   tCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCg                                                                                               >  1:1524212/1‑149 (MQ=255)
                                                    ccTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGc                                                                                              <  1:2557266/149‑1 (MQ=255)
                                                     cTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCa                                                                                             >  1:2372615/1‑149 (MQ=255)
                                                         gCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGa                                                                                         <  1:2402702/149‑1 (MQ=255)
                                                         gCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGa                                                                                         >  2:1895669/1‑149 (MQ=255)
                                                          cAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGAt                                                                                        >  1:3312359/1‑149 (MQ=255)
                                                           aaaCCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGAtt                                                                                       <  1:3380469/149‑1 (MQ=255)
                                                            aaCCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTa                                                                                      >  2:3302077/1‑149 (MQ=255)
                                                            aaCCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGAACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACAAGCAGCGAtaa                                                                                      >  2:2141886/1‑147 (MQ=255)
                                                              ccAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTaaa                                                                                    >  1:373623/1‑149 (MQ=255)
                                                               cAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTaaaa                                                                                   >  1:4103079/1‑149 (MQ=255)
                                                                aGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAAt                                                                                  <  1:3664314/149‑1 (MQ=255)
                                                                  tAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCt                                                                                <  1:3697200/149‑1 (MQ=255)
                                                                     tttATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTAc                                                                             <  1:1854100/149‑1 (MQ=255)
                                                                       tattatTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTg                                                                           <  2:3470986/149‑1 (MQ=255)
                                                                         ttattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGaa                                                                         >  1:2953990/1‑149 (MQ=255)
                                                                          tattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAt                                                                        >  1:4361039/1‑149 (MQ=255)
                                                                          tattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAt                                                                        <  2:3508517/149‑1 (MQ=255)
                                                                           attTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAtt                                                                       <  2:1206700/149‑1 (MQ=255)
                                                                           attTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAtt                                                                       >  2:2018553/1‑149 (MQ=255)
                                                                           attTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAtt                                                                       >  2:2641205/1‑149 (MQ=255)
                                                                              tCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAttctt                                                                    >  1:4320546/1‑149 (MQ=255)
                                                                               cTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAttcttt                                                                   <  2:3160250/149‑1 (MQ=255)
                                                                                ttACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAttctttc                                                                  >  2:4723491/1‑149 (MQ=255)
                                                                                 tACGAACATCATCATGAGTGAAGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAttctttct                                                                 <  1:4532370/149‑1 (MQ=255)
                                                                                    gAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTtat                                                              >  2:4177961/1‑149 (MQ=255)
                                                                                        aTCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAAtt                                                          <  2:867215/149‑1 (MQ=255)
                                                                                        aTCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAAtt                                                          <  2:1744115/149‑1 (MQ=255)
                                                                                        aTCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAAtt                                                          <  2:2931600/149‑1 (MQ=255)
                                                                                          cAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTAt                                                        <  2:3433510/149‑1 (MQ=255)
                                                                                           aGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATg                                                       <  2:1524124/149‑1 (MQ=255)
                                                                                                gAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCa                                                  <  2:538219/149‑1 (MQ=255)
                                                                                                    gACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCAt                                              <  2:3421142/149‑1 (MQ=255)
                                                                                                      cGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATaa                                            <  2:1573950/149‑1 (MQ=255)
                                                                                                           cTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGa                                       >  1:3226641/1‑149 (MQ=255)
                                                                                                            tAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGaa                                      >  2:1612427/1‑149 (MQ=255)
                                                                                                            tAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGaa                                      <  1:4701469/149‑1 (MQ=255)
                                                                                                            tAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGaa                                      <  2:3578438/149‑1 (MQ=255)
                                                                                                            tAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGaa                                      <  1:2195236/149‑1 (MQ=255)
                                                                                                              aGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATg                                    >  1:3078795/1‑149 (MQ=255)
                                                                                                               gCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGc                                   >  1:1013046/1‑149 (MQ=255)
                                                                                                                   tctGTTGCAAGCATTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATa                               <  2:1131497/149‑1 (MQ=255)
                                                                                                                   tctGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATa                               <  1:1172697/149‑1 (MQ=255)
                                                                                                                   tctGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATa                               <  2:446013/149‑1 (MQ=255)
                                                                                                                    ctGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAg                              >  1:3526920/1‑149 (MQ=255)
                                                                                                                     tGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGa                             <  1:287030/149‑1 (MQ=255)
                                                                                                                      gTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGAc                            <  2:124697/149‑1 (MQ=255)
                                                                                                                      gTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGAc                            <  2:4749329/149‑1 (MQ=255)
                                                                                                                        tGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACAt                          <  2:3224158/149‑1 (MQ=255)
                                                                                                                        tGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACAt                          <  2:493472/149‑1 (MQ=255)
                                                                                                                        tGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACAt                          <  1:3890338/149‑1 (MQ=255)
                                                                                                                         gcaagcaaTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACAtt                         <  2:3818606/149‑1 (MQ=255)
                                                                                                                          caagcaaTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTa                        <  1:2012237/149‑1 (MQ=255)
                                                                                                                            agcaaTGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACt                      >  1:4365823/1‑149 (MQ=255)
                                                                                                                            agcaaTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACt                      <  1:1108016/149‑1 (MQ=255)
                                                                                                                              caaTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTa                    >  1:2730121/1‑149 (MQ=255)
                                                                                                                               aaTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATCATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTaa                   >  2:4008623/1‑149 (MQ=255)
                                                                                                                                aTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAAt                  <  2:2537492/149‑1 (MQ=255)
                                                                                                                                 tGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATg                 <  2:2814507/149‑1 (MQ=255)
                                                                                                                                    tATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGaaa              <  2:425146/149‑1 (MQ=255)
                                                                                                                                     aTTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAAt             >  1:1066759/1‑149 (MQ=255)
                                                                                                                                     aTTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAAt             <  2:3930907/149‑1 (MQ=255)
                                                                                                                                      ttGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATa            >  1:3153841/1‑149 (MQ=255)
                                                                                                                                      ttGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATa            >  1:4264440/1‑149 (MQ=255)
                                                                                                                                         ctgcACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGa         <  1:580399/149‑1 (MQ=255)
                                                                                                                                           gcACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGaaa       <  1:179360/149‑1 (MQ=255)
                                                                                                                                           gcACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGaaa       <  1:885084/149‑1 (MQ=255)
                                                                                                                                           gcACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGaaa       <  2:1939860/149‑1 (MQ=255)
                                                                                                                                             aCCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAg     <  1:3841232/149‑1 (MQ=255)
                                                                                                                                              ccAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAATTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAgg    <  1:2926631/149‑1 (MQ=255)
                                                                                                                                                aaTCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCa  >  2:684199/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CAAACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCA  >  CP009273/2101764‑2102056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACAAACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAGGC  >  CP009273/2101763‑2102061
                                                                                                                                                     |                                                                                                                                                     
ACAAACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCG                                                                                                                                                       >  SRR19973071.3445645/1‑150 (MQ=60)
 CAAACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGC                                                                                                                                                      >  SRR19973071.3295094/1‑150 (MQ=60)
    ACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTT                                                                                                                                                   <  SRR19973071.1102784/150‑1 (MQ=60)
    ACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTT                                                                                                                                                   <  SRR19973071.3918241/150‑1 (MQ=60)
     CGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTA                                                                                                                                                  <  SRR19973071.1154197/150‑1 (MQ=60)
         ACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAA                                                                                                                                              >  SRR19973071.3563692/1‑150 (MQ=60)
           CAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGA                                                                                                                                            >  SRR19973071.2181851/1‑150 (MQ=60)
           CAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGA                                                                                                                                            >  SRR19973071.2181814/1‑150 (MQ=60)
            AAACAAACCAGAACCAACAATGATATACTCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAG                                                                                                                                           <  SRR19973071.1251633/150‑1 (MQ=60)
               CAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTG                                                                                                                                        <  SRR19973071.3710229/150‑1 (MQ=60)
                AAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGT                                                                                                                                       <  SRR19973071.1256021/150‑1 (MQ=60)
                AAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGT                                                                                                                                       >  SRR19973071.816310/1‑150 (MQ=60)
                 AACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTT                                                                                                                                      >  SRR19973071.3350552/1‑150 (MQ=60)
                 AACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTT                                                                                                                                      <  SRR19973071.4117852/150‑1 (MQ=60)
                  ACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTA                                                                                                                                     >  SRR19973071.3451534/1‑150 (MQ=60)
                       AACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGA                                                                                                                                <  SRR19973071.1487189/150‑1 (MQ=60)
                       AACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTGTAAAAAGAGTTGTTAGCGGA                                                                                                                                >  SRR19973071.1926131/1‑150 (MQ=60)
                          CAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAA                                                                                                                             >  SRR19973071.4611034/1‑150 (MQ=60)
                          CAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAA                                                                                                                             >  SRR19973071.4611049/1‑150 (MQ=60)
                          CAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAA                                                                                                                             >  SRR19973071.4611054/1‑150 (MQ=60)
                            ACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAAT                                                                                                                           >  SRR19973071.3506889/1‑150 (MQ=60)
                             CAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATC                                                                                                                          <  SRR19973071.3504306/150‑1 (MQ=60)
                             CAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATC                                                                                                                          >  SRR19973071.3969302/1‑150 (MQ=60)
                               ATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAA                                                                                                                        >  SRR19973071.3891785/1‑150 (MQ=60)
                                TGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAAC                                                                                                                       <  SRR19973071.702368/150‑1 (MQ=60)
                                   TATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAAC                                                                                                                    <  SRR19973071.2754610/150‑1 (MQ=60)
                                    ATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTGTAAAAAGAGTTGTTAGCGGAAAAATCAACAACA                                                                                                                   >  SRR19973071.4163767/1‑150 (MQ=60)
                                      AATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAA                                                                                                                 >  SRR19973071.4744877/1‑150 (MQ=60)
                                         CGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTC                                                                                                              >  SRR19973071.4377548/1‑150 (MQ=60)
                                            ACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAAC                                                                                                           <  SRR19973071.934542/150‑1 (MQ=60)
                                            ACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAAC                                                                                                           >  SRR19973071.1849642/1‑150 (MQ=60)
                                              ATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAA                                                                                                         >  SRR19973071.4038281/1‑150 (MQ=60)
                                              ATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAA                                                                                                         >  SRR19973071.4038538/1‑150 (MQ=60)
                                               TAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAA                                                                                                        >  SRR19973071.3715569/1‑150 (MQ=60)
                                                   ATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCG                                                                                                    >  SRR19973071.1529702/1‑150 (MQ=60)
                                                   ATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCG                                                                                                    >  SRR19973071.1529614/1‑150 (MQ=60)
                                                   ATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCG                                                                                                    >  SRR19973071.1327142/1‑150 (MQ=60)
                                                     CCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCA                                                                                                  <  SRR19973071.2566551/150‑1 (MQ=60)
                                                     CCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCA                                                                                                  >  SRR19973071.2381185/1‑150 (MQ=60)
                                                     CCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCA                                                                                                  >  SRR19973071.2381247/1‑150 (MQ=60)
                                                      CTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAG                                                                                                 <  SRR19973071.3122271/150‑1 (MQ=60)
                                                          GCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGAT                                                                                             >  SRR19973071.3324103/1‑150 (MQ=60)
                                                          GCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGAT                                                                                             >  SRR19973071.1726986/1‑150 (MQ=60)
                                                          GCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGAT                                                                                             <  SRR19973071.2411360/150‑1 (MQ=60)
                                                            AAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTA                                                                                           <  SRR19973071.3392463/150‑1 (MQ=60)
                                                             AACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAA                                                                                          >  SRR19973071.113064/1‑150 (MQ=60)
                                                              ACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGACAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAA                                                                                         >  SRR19973071.2824450/1‑150 (MQ=60)
                                                              ACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAA                                                                                         >  SRR19973071.2824728/1‑150 (MQ=60)
                                                              ACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAA                                                                                         >  SRR19973071.374807/1‑150 (MQ=60)
                                                              ACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAA                                                                                         >  SRR19973071.374681/1‑150 (MQ=60)
                                                               CCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAA                                                                                        >  SRR19973071.4117660/1‑150 (MQ=60)
                                                                 AGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATC                                                                                      <  SRR19973071.3677237/150‑1 (MQ=60)
                                                                   TAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTT                                                                                    <  SRR19973071.563787/150‑1 (MQ=60)
                                                                   TAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTT                                                                                    <  SRR19973071.3710291/150‑1 (MQ=60)
                                                                      TTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACT                                                                                 <  SRR19973071.1861058/150‑1 (MQ=60)
                                                                      TTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACT                                                                                 <  SRR19973071.1861052/150‑1 (MQ=60)
                                                                      TTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACT                                                                                 <  SRR19973071.1860960/150‑1 (MQ=60)
                                                                      TTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACT                                                                                 <  SRR19973071.2075964/150‑1 (MQ=60)
                                                                      TTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACT                                                                                 <  SRR19973071.2254747/150‑1 (MQ=60)
                                                                       TTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTG                                                                                <  SRR19973071.3336623/150‑1 (MQ=60)
                                                                         ATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAA                                                                              <  SRR19973071.1068703/150‑1 (MQ=60)
                                                                         ATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAA                                                                              >  SRR19973071.2964572/1‑150 (MQ=60)
                                                                         ATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAA                                                                              >  SRR19973071.2964579/1‑150 (MQ=60)
                                                                          TTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAT                                                                             >  SRR19973071.4376388/1‑150 (MQ=60)
                                                                              TTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTT                                                                         >  SRR19973071.4335801/1‑150 (MQ=60)
                                                                              TTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCGT                                                                         <  SRR19973071.3561930/150‑1 (MQ=60)
                                                                                  TACGAACATCATCATGAGTGAAGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTT                                                                     <  SRR19973071.4548512/150‑1 (MQ=60)
                                                                                    CGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTAT                                                                   <  SRR19973071.9138/150‑1 (MQ=60)
                                                                                     GAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATA                                                                  >  SRR19973071.2745548/1‑150 (MQ=60)
                                                                                      AACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAA                                                                 >  SRR19973071.2939039/1‑150 (MQ=60)
                                                                                      AACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAA                                                                 <  SRR19973071.219776/150‑1 (MQ=60)
                                                                                               ATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTC                                                        >  SRR19973071.2389112/1‑150 (MQ=60)
                                                                                                TGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCA                                                       >  SRR19973071.4169345/1‑150 (MQ=60)
                                                                                                     GACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATA                                                  <  SRR19973071.1595619/150‑1 (MQ=60)
                                                                                                     GACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATA                                                  >  SRR19973071.195562/1‑150 (MQ=60)
                                                                                                       CGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAAC                                                <  SRR19973071.2137431/150‑1 (MQ=60)
                                                                                                           ACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGA                                            >  SRR19973071.3238005/1‑150 (MQ=60)
                                                                                                           ACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGA                                            >  SRR19973071.3237980/1‑150 (MQ=60)
                                                                                                           ACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGA                                            >  SRR19973071.3237884/1‑150 (MQ=60)
                                                                                                            CTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAA                                           <  SRR19973071.4336754/150‑1 (MQ=60)
                                                                                                            CTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAA                                           <  SRR19973071.4336707/150‑1 (MQ=60)
                                                                                                             TAAGCACTCTGTTTCAAGCAATGTTATTGCTTCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAAT                                          <  SRR19973071.3245680/150‑1 (MQ=60)
                                                                                                             TAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAAT                                          <  SRR19973071.4456581/150‑1 (MQ=60)
                                                                                                             TAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAAT                                          <  SRR19973071.2203351/150‑1 (MQ=60)
                                                                                                             TAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAAT                                          <  SRR19973071.4718158/150‑1 (MQ=60)
                                                                                                              AAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATG                                         >  SRR19973071.3089692/1‑150 (MQ=60)
                                                                                                              AAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATG                                         >  SRR19973071.3357090/1‑150 (MQ=60)
                                                                                                               AGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGC                                        <  SRR19973071.3423671/150‑1 (MQ=60)
                                                                                                               AGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGC                                        >  SRR19973071.1016723/1‑150 (MQ=60)
                                                                                                                   CTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATA                                    <  SRR19973071.753404/150‑1 (MQ=60)
                                                                                                                    TCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAG                                   <  SRR19973071.1176743/150‑1 (MQ=60)
                                                                                                                    TCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAG                                   >  SRR19973071.3539272/1‑150 (MQ=60)
                                                                                                                      TGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGAC                                 <  SRR19973071.288304/150‑1 (MQ=60)
                                                                                                                      TGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGAC                                 <  SRR19973071.288074/150‑1 (MQ=60)
                                                                                                                      TGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGAC                                 <  SRR19973071.4740405/150‑1 (MQ=60)
                                                                                                                       GTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACA                                >  SRR19973071.678441/1‑150 (MQ=60)
                                                                                                                        TTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACAT                               <  SRR19973071.3829818/150‑1 (MQ=60)
                                                                                                                         TGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATT                              >  SRR19973071.4585114/1‑150 (MQ=60)
                                                                                                                         TGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATT                              <  SRR19973071.3904127/150‑1 (MQ=60)
                                                                                                                         TGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATT                              <  SRR19973071.3904071/150‑1 (MQ=60)
                                                                                                                         TGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATT                              <  SRR19973071.3903577/150‑1 (MQ=60)
                                                                                                                           CAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTAC                            <  SRR19973071.2019526/150‑1 (MQ=60)
                                                                                                                           CAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTAC                            <  SRR19973071.4417122/150‑1 (MQ=60)
                                                                                                                            AAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACT                           >  SRR19973071.4381181/1‑150 (MQ=60)
                                                                                                                             AGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTT                          <  SRR19973071.1111917/150‑1 (MQ=60)
                                                                                                                              GCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTA                         >  SRR19973071.2739820/1‑150 (MQ=60)
                                                                                                                              GCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTA                         >  SRR19973071.4017124/1‑150 (MQ=60)
                                                                                                                                AATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAAT                       <  SRR19973071.2449531/150‑1 (MQ=60)
                                                                                                                                AATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAAT                       <  SRR19973071.2734532/150‑1 (MQ=60)
                                                                                                                                   GTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTATCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTAATTCTTATAATTATGGGTCAGCATAACTTGAATGCCAAAGACATTACTTAATGAA                    >  SRR19973071.1339502/1‑150 (MQ=60)
                                                                                                                                    TTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAA                   <  SRR19973071.2610410/150‑1 (MQ=60)
                                                                                                                                     TATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAAT                  >  SRR19973071.1070566/1‑150 (MQ=60)
                                                                                                                                      ATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATA                 >  SRR19973071.4279560/1‑150 (MQ=60)
                                                                                                                                      ATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATA                 >  SRR19973071.3164874/1‑150 (MQ=60)
                                                                                                                                      ATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATA                 <  SRR19973071.412713/150‑1 (MQ=60)
                                                                                                                                      ATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATA                 >  SRR19973071.4279709/1‑150 (MQ=60)
                                                                                                                                          CTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAA             <  SRR19973071.582571/150‑1 (MQ=60)
                                                                                                                                           TGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAA            <  SRR19973071.4749579/150‑1 (MQ=60)
                                                                                                                                            GCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAA           <  SRR19973071.180111/150‑1 (MQ=60)
                                                                                                                                            GCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAA           <  SRR19973071.888262/150‑1 (MQ=60)
                                                                                                                                              ACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGG         <  SRR19973071.3854843/150‑1 (MQ=60)
                                                                                                                                              ACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGG         <  SRR19973071.3157896/150‑1 (MQ=60)
                                                                                                                                               CCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAATTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGC        <  SRR19973071.2937155/150‑1 (MQ=60)
                                                                                                                                               CCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAATTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGC        <  SRR19973071.2937118/150‑1 (MQ=60)
                                                                                                                                                  ATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGA     >  SRR19973071.2584757/1‑150 (MQ=60)
                                                                                                                                                    CGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAGG   <  SRR19973071.221337/150‑1 (MQ=60)
                                                                                                                                                     GCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAGGC  >  SRR19973071.4174088/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
ACAAACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAGGC  >  CP009273/2101763‑2102061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑