Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,101,912 T→G 100% E385A (GAG→GCG)  wzxB ← putative polisoprenol‑linked O‑antigen transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,101,9120TG96.6% 915.8 / 7.2 236E385A (GAG→GCG) wzxBputative polisoprenol‑linked O‑antigen transporter
Reads supporting (aligned to +/- strand):  ref base T (4/4);  new base G (104/124);  total (108/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 242 total aligned reads displayed.

ACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCA  >  CP009273/2101767‑2102056
                                                                                                                                                 |                                                                                                                                                
aCGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATcgct                                                                                                                                                <  1:2777365/148‑1 (MQ=255)
aCGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATcgct                                                                                                                                                <  1:3300968/148‑1 (MQ=255)
aCGGCACCAAACAAACCAGAACCAACAATGATATAATCGTAAATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATcgct                                                                                                                                                <  1:3266770/148‑1 (MQ=255)
 cGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCtt                                                                                                                                               >  2:24970/1‑148 (MQ=255)
   gCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTa                                                                                                                                             >  1:237417/1‑148 (MQ=255)
      ccAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTaaaa                                                                                                                                          <  2:2917325/148‑1 (MQ=255)
       caaacaaaCCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTaaaaa                                                                                                                                         >  1:1053539/1‑148 (MQ=255)
        aaacaaacCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTaaaaat                                                                                                                                        >  2:1680642/1‑147 (MQ=255)
        aaacaaacCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAag                                                                                                                                        >  1:2240371/1‑148 (MQ=255)
          acaaacCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCACTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAagag                                                                                                                                      <  2:2725930/148‑1 (MQ=255)
             aacCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAgttg                                                                                                                                   <  1:595397/148‑1 (MQ=255)
             aacCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAgttg                                                                                                                                   >  2:2868589/1‑148 (MQ=255)
               ccAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAgttgtt                                                                                                                                 >  1:183316/1‑148 (MQ=255)
               ccAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAgttgtt                                                                                                                                 >  1:1980752/1‑148 (MQ=255)
                cAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTa                                                                                                                                <  2:18926/148‑1 (MQ=255)
                 aGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAg                                                                                                                               >  2:608449/1‑148 (MQ=255)
                 aGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAg                                                                                                                               <  2:3203019/148‑1 (MQ=255)
                   aaCCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCg                                                                                                                             >  2:268614/1‑148 (MQ=255)
                   aaCCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCg                                                                                                                             >  2:2157499/1‑148 (MQ=255)
                    aCCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCgg                                                                                                                            <  2:312133/148‑1 (MQ=255)
                      caacaaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGaa                                                                                                                          >  1:182865/1‑148 (MQ=255)
                       aacaaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGaaa                                                                                                                         >  2:393425/1‑148 (MQ=255)
                           aTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATc                                                                                                                     >  2:2980973/1‑148 (MQ=255)
                           aTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATc                                                                                                                     >  2:2395690/1‑148 (MQ=255)
                               tataATCGTACATAAAAGCCTCCGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATcaaca                                                                                                                 <  2:2247738/148‑1 (MQ=255)
                                   aTCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATcaacaacaa                                                                                                             >  2:1655232/1‑148 (MQ=255)
                                     cGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAAc                                                                                                           <  1:2283388/148‑1 (MQ=255)
                                        aCATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTca                                                                                                        >  1:675924/1‑148 (MQ=255)
                                         cATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTcaa                                                                                                       <  2:51499/148‑1 (MQ=255)
                                           tAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTcaaca                                                                                                     >  1:2889942/1‑148 (MQ=255)
                                           tAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTcaaca                                                                                                     >  1:1115241/1‑148 (MQ=255)
                                              aaTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAc                                                                                                  <  2:1433920/148‑1 (MQ=255)
                                              aaTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAc                                                                                                  >  1:2949682/1‑148 (MQ=255)
                                              aaTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAc                                                                                                  >  2:774226/1‑148 (MQ=255)
                                              aaTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAc                                                                                                  >  2:3407607/1‑148 (MQ=255)
                                               aTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAcc                                                                                                 >  2:1443083/1‑148 (MQ=255)
                                               aTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAcc                                                                                                 <  2:840820/148‑1 (MQ=255)
                                               aTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAcc                                                                                                 <  1:2343103/148‑1 (MQ=255)
                                                 ccTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCg                                                                                               >  1:1741585/1‑148 (MQ=255)
                                                  cTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCTATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGc                                                                                              <  2:1822319/148‑1 (MQ=255)
                                                    cAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAg                                                                                            <  2:960751/148‑1 (MQ=255)
                                                      gCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCg                                                                                          >  1:21039/1‑148 (MQ=255)
                                                       cAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGa                                                                                         >  1:2907117/1‑148 (MQ=255)
                                                         aaCCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGAtt                                                                                       <  1:3202748/148‑1 (MQ=255)
                                                         aaCCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGAtt                                                                                       <  2:2468806/148‑1 (MQ=255)
                                                            cAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTaaa                                                                                    <  1:1168757/148‑1 (MQ=255)
                                                              gTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAAt                                                                                  >  2:118256/1‑148 (MQ=255)
                                                                aaTTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCt                                                                                >  2:3105392/1‑148 (MQ=255)
                                                                  tttATTAATTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTa                                                                              >  1:2825441/1‑148 (MQ=255)
                                                                   ttattaTTTCGTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTAc                                                                             <  1:2400081/148‑1 (MQ=255)
                                                                    tattatTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACt                                                                            >  1:2067576/1‑148 (MQ=255)
                                                                    tattatTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAACCGCAGCGATTAAAATCTTACt                                                                            >  2:2791794/1‑148 (MQ=255)
                                                                     attattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTg                                                                           <  1:1321498/148‑1 (MQ=255)
                                                                      ttattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGa                                                                          <  2:2339641/148‑1 (MQ=255)
                                                                      ttattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGa                                                                          <  1:2415188/148‑1 (MQ=255)
                                                                       tattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGaa                                                                         >  2:888650/1‑148 (MQ=255)
                                                                       tattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGaa                                                                         <  1:2554047/148‑1 (MQ=255)
                                                                         tttCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAtt                                                                       <  2:1115241/148‑1 (MQ=255)
                                                                               aCGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAttctttct                                                                 <  1:1210038/148‑1 (MQ=255)
                                                                                cGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATtctttctt                                                                <  1:1564553/148‑1 (MQ=255)
                                                                                cGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATtctttctt                                                                <  1:3325737/148‑1 (MQ=255)
                                                                                cGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATtctttctt                                                                >  1:986601/1‑148 (MQ=255)
                                                                                cGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATtctttctt                                                                >  2:1012316/1‑148 (MQ=255)
                                                                                   aCATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTtata                                                             <  2:2240183/148‑1 (MQ=255)
                                                                                    cATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATaa                                                            <  2:3290044/148‑1 (MQ=255)
                                                                                      tCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAAtt                                                          >  1:3459074/1‑148 (MQ=255)
                                                                                       cAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTa                                                         <  1:1540523/148‑1 (MQ=255)
                                                                                        aGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTAt                                                        >  2:2657849/1‑148 (MQ=255)
                                                                                        aGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTAt                                                        <  2:255438/148‑1 (MQ=255)
                                                                                        aGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTAt                                                        <  2:2049677/148‑1 (MQ=255)
                                                                                         gCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATg                                                       <  1:2066574/148‑1 (MQ=255)
                                                                                         gCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATg                                                       <  2:3390472/148‑1 (MQ=255)
                                                                                          cATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATgg                                                      >  2:2607719/1‑148 (MQ=255)
                                                                                           aTGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATggg                                                     <  1:452983/148‑1 (MQ=255)
                                                                                            tGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGt                                                    <  2:1537458/148‑1 (MQ=255)
                                                                                            tGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGt                                                    <  2:1848977/148‑1 (MQ=255)
                                                                                            tGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACAAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGt                                                    <  1:534702/148‑1 (MQ=255)
                                                                                             gAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTc                                                   >  2:3463410/1‑148 (MQ=255)
                                                                                                 gACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCa                                               <  1:2995566/148‑1 (MQ=255)
                                                                                                      aaCTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACt                                          >  1:1407296/1‑148 (MQ=255)
                                                                                                         tAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGa                                       <  1:791982/148‑1 (MQ=255)
                                                                                                           aGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAAt                                     >  2:665332/1‑148 (MQ=255)
                                                                                                            gCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATg                                    <  2:23578/148‑1 (MQ=255)
                                                                                                              aCTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGcc                                  >  1:615789/1‑148 (MQ=255)
                                                                                                               ctctGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCa                                 >  2:3238018/1‑148 (MQ=255)
                                                                                                                  tGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAg                              <  1:2003505/148‑1 (MQ=255)
                                                                                                                  tGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAg                              <  2:183316/148‑1 (MQ=255)
                                                                                                                    ttGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGAc                            >  1:3399858/1‑148 (MQ=255)
                                                                                                                     tGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACa                           <  1:1279668/148‑1 (MQ=255)
                                                                                                                         agcaaTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTAc                       >  1:2514582/1‑148 (MQ=255)
                                                                                                                          gcaaTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACt                      >  1:3120884/1‑148 (MQ=255)
                                                                                                                            aaTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTa                    >  2:1503355/1‑148 (MQ=255)
                                                                                                                                  aTTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGaaa              <  1:1729051/148‑1 (MQ=255)
                                                                                                                                      ctgcACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAg          <  1:3414372/148‑1 (MQ=255)
                                                                                                                                       tgcACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGa         <  2:1248492/148‑1 (MQ=255)
                                                                                                                                         cACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACACACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGaaa       <  2:1364539/148‑1 (MQ=255)
                                                                                                                                         cACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGaaa       >  1:217849/1‑148 (MQ=255)
                                                                                                                                            cAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAgg    >  1:549948/1‑148 (MQ=255)
                                                                                                                                             aaTCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGTGTCAGCATAACTTGAATGACATAGACATTACTTAATGAAATAAGAAAAGGc   >  1:1056269/1‑148 (MQ=255)
                                                                                                                                             aaTCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGAAATTACTTAATGAAATAAGAAAAGGc   >  1:1848219/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCA  >  CP009273/2101767‑2102056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACAAACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAG  >  CP009273/2101763‑2102059
                                                                                                                                                     |                                                                                                                                                   
ACAAACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCG                                                                                                                                                     >  SRR19973067.1390859/1‑150 (MQ=60)
   AACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTT                                                                                                                                                  <  SRR19973067.3098746/150‑1 (MQ=60)
    ACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTT                                                                                                                                                 <  SRR19973067.2795306/150‑1 (MQ=60)
    ACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTT                                                                                                                                                 <  SRR19973067.3321763/150‑1 (MQ=60)
    ACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTT                                                                                                                                                 <  SRR19973067.3321796/150‑1 (MQ=60)
    ACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTT                                                                                                                                                 <  SRR19973067.3321978/150‑1 (MQ=60)
    ACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTAAATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTT                                                                                                                                                 <  SRR19973067.3287154/150‑1 (MQ=60)
     CGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTA                                                                                                                                                >  SRR19973067.239088/1‑150 (MQ=60)
         ACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAA                                                                                                                                            >  SRR19973067.1060762/1‑150 (MQ=60)
          CCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAG                                                                                                                                           <  SRR19973067.998299/150‑1 (MQ=60)
          CCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAG                                                                                                                                           >  SRR19973067.2255271/1‑150 (MQ=60)
          CCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAG                                                                                                                                           >  SRR19973067.2255083/1‑150 (MQ=60)
          CCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAG                                                                                                                                           <  SRR19973067.66905/150‑1 (MQ=60)
            AAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAG                                                                                                                                         >  SRR19973067.2862243/1‑150 (MQ=60)
            AAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAG                                                                                                                                         >  SRR19973067.823012/1‑150 (MQ=60)
                 AACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTT                                                                                                                                    <  SRR19973067.599175/150‑1 (MQ=60)
                 AACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTT                                                                                                                                    <  SRR19973067.2395490/150‑1 (MQ=60)
                 AACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTT                                                                                                                                    >  SRR19973067.1994160/1‑150 (MQ=60)
                 AACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTT                                                                                                                                    >  SRR19973067.184767/1‑150 (MQ=60)
                 AACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTT                                                                                                                                    >  SRR19973067.184734/1‑150 (MQ=60)
                    CAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGC                                                                                                                                 >  SRR19973067.2498543/1‑150 (MQ=60)
                     AGAACCAACACAGAGATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCG                                                                                                                                <  SRR19973067.2664094/150‑1 (MQ=60)
                     AGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCG                                                                                                                                >  SRR19973067.2808731/1‑150 (MQ=60)
                      GAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGG                                                                                                                               >  SRR19973067.1768086/1‑150 (MQ=60)
                        ACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAA                                                                                                                             >  SRR19973067.184314/1‑150 (MQ=60)
                          CAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAA                                                                                                                           >  SRR19973067.23944/1‑150 (MQ=60)
                            ACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAAT                                                                                                                         >  SRR19973067.2292459/1‑150 (MQ=60)
                            ACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAAT                                                                                                                         <  SRR19973067.206531/150‑1 (MQ=60)
                              AATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCA                                                                                                                       <  SRR19973067.1347431/150‑1 (MQ=60)
                                TGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAAC                                                                                                                     <  SRR19973067.596739/150‑1 (MQ=60)
                                  ATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAA                                                                                                                   >  SRR19973067.2918353/1‑150 (MQ=60)
                                         CGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTC                                                                                                            <  SRR19973067.2298485/150‑1 (MQ=60)
                                          GTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCA                                                                                                           >  SRR19973067.3403583/1‑150 (MQ=60)
                                          GTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCA                                                                                                           >  SRR19973067.680563/1‑150 (MQ=60)
                                           TACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAA                                                                                                          >  SRR19973067.179836/1‑150 (MQ=60)
                                            ACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAAC                                                                                                         <  SRR19973067.1969888/150‑1 (MQ=60)
                                             CATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACA                                                                                                        >  SRR19973067.1122806/1‑150 (MQ=60)
                                             CATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACA                                                                                                        >  SRR19973067.2908410/1‑150 (MQ=60)
                                             CATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACA                                                                                                        >  SRR19973067.573308/1‑150 (MQ=60)
                                                AAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAC                                                                                                     >  SRR19973067.2968346/1‑150 (MQ=60)
                                                  AATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCC                                                                                                   <  SRR19973067.125233/150‑1 (MQ=60)
                                                  AATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCC                                                                                                   <  SRR19973067.457030/150‑1 (MQ=60)
                                                  AATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCC                                                                                                   <  SRR19973067.300487/150‑1 (MQ=60)
                                                  AATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCC                                                                                                   <  SRR19973067.124235/150‑1 (MQ=60)
                                                   ATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCG                                                                                                  <  SRR19973067.2358607/150‑1 (MQ=60)
                                                   ATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCG                                                                                                  <  SRR19973067.2358425/150‑1 (MQ=60)
                                                   ATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCG                                                                                                  >  SRR19973067.1753482/1‑150 (MQ=60)
                                                        CAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCG                                                                                             <  SRR19973067.2019938/150‑1 (MQ=60)
                                                        CAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCG                                                                                             >  SRR19973067.21355/1‑150 (MQ=60)
                                                        CAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCG                                                                                             <  SRR19973067.2976469/150‑1 (MQ=60)
                                                         AGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGA                                                                                            >  SRR19973067.2925644/1‑150 (MQ=60)
                                                          GCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGAT                                                                                           <  SRR19973067.3481014/150‑1 (MQ=60)
                                                           CAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATT                                                                                          >  SRR19973067.713937/1‑150 (MQ=60)
                                                             AACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAA                                                                                        <  SRR19973067.3058978/150‑1 (MQ=60)
                                                             AACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAA                                                                                        <  SRR19973067.3222944/150‑1 (MQ=60)
                                                                CAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAAT                                                                                     <  SRR19973067.2960030/150‑1 (MQ=60)
                                                                CAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAAT                                                                                     <  SRR19973067.1176329/150‑1 (MQ=60)
                                                                CAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAAT                                                                                     <  SRR19973067.191728/150‑1 (MQ=60)
                                                                CAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAAT                                                                                     <  SRR19973067.1176513/150‑1 (MQ=60)
                                                                CAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAAT                                                                                     <  SRR19973067.988291/150‑1 (MQ=60)
                                                                    AATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCAGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAAACAACAACAAACTCAACAAACCCGCAACGATTAAAATCTTA                                                                                 >  SRR19973067.3237744/1‑150 (MQ=60)
                                                                    AATTTATTAATTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTA                                                                                 >  SRR19973067.2843619/1‑150 (MQ=60)
                                                                     ATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTAC                                                                                >  SRR19973067.1654465/1‑150 (MQ=60)
                                                                      TTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACT                                                                               <  SRR19973067.2514238/150‑1 (MQ=60)
                                                                      TTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACT                                                                               >  SRR19973067.2081320/1‑150 (MQ=60)
                                                                      TTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACT                                                                               >  SRR19973067.2081229/1‑150 (MQ=60)
                                                                      TTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACT                                                                               <  SRR19973067.3481128/150‑1 (MQ=60)
                                                                       TTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTG                                                                              <  SRR19973067.2415730/150‑1 (MQ=60)
                                                                       TTATTATTTCGTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTG                                                                              <  SRR19973067.2415729/150‑1 (MQ=60)
                                                                         ATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAA                                                                            <  SRR19973067.1330483/150‑1 (MQ=60)
                                                                         ATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAA                                                                            <  SRR19973067.1330519/150‑1 (MQ=60)
                                                                          TTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAT                                                                           <  SRR19973067.2431003/150‑1 (MQ=60)
                                                                          TTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAT                                                                           <  SRR19973067.1132975/150‑1 (MQ=60)
                                                                           TATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATT                                                                          <  SRR19973067.2570748/150‑1 (MQ=60)
                                                                              TTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTT                                                                       <  SRR19973067.2886956/150‑1 (MQ=60)
                                                                                CTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTC                                                                     <  SRR19973067.1528536/150‑1 (MQ=60)
                                                                                  TACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTT                                                                   >  SRR19973067.993386/1‑150 (MQ=60)
                                                                                   ACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTA                                                                  <  SRR19973067.1218008/150‑1 (MQ=60)
                                                                                    CGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTAT                                                                 <  SRR19973067.1575257/150‑1 (MQ=60)
                                                                                    CGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTAT                                                                 <  SRR19973067.3346772/150‑1 (MQ=60)
                                                                                     GAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATA                                                                <  SRR19973067.1746015/150‑1 (MQ=60)
                                                                                      AACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAA                                                               >  SRR19973067.1469678/1‑150 (MQ=60)
                                                                                        CATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATT                                                             >  SRR19973067.3481068/1‑150 (MQ=60)
                                                                                        CATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATT                                                             <  SRR19973067.2009296/150‑1 (MQ=60)
                                                                                        CATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATT                                                             >  SRR19973067.3188458/1‑150 (MQ=60)
                                                                                           CAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATG                                                          <  SRR19973067.1550963/150‑1 (MQ=60)
                                                                                             GCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGG                                                        <  SRR19973067.3538987/150‑1 (MQ=60)
                                                                                             GCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGG                                                        <  SRR19973067.2080315/150‑1 (MQ=60)
                                                                                              CATGAGTGACGTAACTAAGCACTCTGTTGCTAGCAATGTTATTGCTGCAACAATCGATTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGAGATTAAAATCTTACTGAATTCTTTCTTATAATTATGTGT                                                       <  SRR19973067.1692228/150‑1 (MQ=60)
                                                                                               ATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTC                                                      <  SRR19973067.2258112/150‑1 (MQ=60)
                                                                                               ATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTC                                                      <  SRR19973067.456123/150‑1 (MQ=60)
                                                                                                TGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACAAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCA                                                     <  SRR19973067.538280/150‑1 (MQ=60)
                                                                                                   GTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCA                                                  <  SRR19973067.1848829/150‑1 (MQ=60)
                                                                                                     GACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATA                                                <  SRR19973067.3014553/150‑1 (MQ=60)
                                                                                                     GACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATA                                                <  SRR19973067.2139208/150‑1 (MQ=60)
                                                                                                        GTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACT                                             >  SRR19973067.1416833/1‑150 (MQ=60)
                                                                                                          AACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTG                                           >  SRR19973067.1104264/1‑150 (MQ=60)
                                                                                                          AACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTG                                           >  SRR19973067.2771213/1‑150 (MQ=60)
                                                                                                           ACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGA                                          >  SRR19973067.2097493/1‑150 (MQ=60)
                                                                                                             TAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAAT                                        <  SRR19973067.797455/150‑1 (MQ=60)
                                                                                                                GCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCC                                     >  SRR19973067.619801/1‑150 (MQ=60)
                                                                                                                GCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCC                                     >  SRR19973067.619811/1‑150 (MQ=60)
                                                                                                                   CTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATA                                  <  SRR19973067.3177442/150‑1 (MQ=60)
                                                                                                                      TGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGAC                               >  SRR19973067.3421524/1‑150 (MQ=60)
                                                                                                                      TGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGAC                               >  SRR19973067.3421507/1‑150 (MQ=60)
                                                                                                                      TGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGAC                               <  SRR19973067.2017005/150‑1 (MQ=60)
                                                                                                                         TGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATT                            <  SRR19973067.669877/150‑1 (MQ=60)
                                                                                                                         TGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATT                            <  SRR19973067.1288386/150‑1 (MQ=60)
                                                                                                                           CAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTAC                          >  SRR19973067.2531071/1‑150 (MQ=60)
                                                                                                                           CAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTAC                          >  SRR19973067.2531180/1‑150 (MQ=60)
                                                                                                                            AAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACT                         >  SRR19973067.3140721/1‑150 (MQ=60)
                                                                                                                              GCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTA                       <  SRR19973067.2315250/150‑1 (MQ=60)
                                                                                                                                AATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAAT                     <  SRR19973067.119379/150‑1 (MQ=60)
                                                                                                                                   GTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAA                  >  SRR19973067.1570611/1‑150 (MQ=60)
                                                                                                                                   GTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAA                  >  SRR19973067.3302860/1‑150 (MQ=60)
                                                                                                                                      ATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATA               <  SRR19973067.1740891/150‑1 (MQ=60)
                                                                                                                                          CTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAA           <  SRR19973067.551741/150‑1 (MQ=60)
                                                                                                                                          CTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAA           <  SRR19973067.3436078/150‑1 (MQ=60)
                                                                                                                                          CTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAA           <  SRR19973067.3436108/150‑1 (MQ=60)
                                                                                                                                           TGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAA          >  SRR19973067.219434/1‑150 (MQ=60)
                                                                                                                                           TGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAA          >  SRR19973067.219450/1‑150 (MQ=60)
                                                                                                                                              ACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGG       >  SRR19973067.553575/1‑150 (MQ=60)
                                                                                                                                               CCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGTGTCAGCATAACTTGAATGACATAGACATTACTTAATGAAATAAGAAAAGGC      >  SRR19973067.1063510/1‑150 (MQ=60)
                                                                                                                                               CCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGC      >  SRR19973067.1063332/1‑150 (MQ=60)
                                                                                                                                               CCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGAAATTACTTAATGAAATAAGAAAAGGC      >  SRR19973067.1860816/1‑150 (MQ=60)
                                                                                                                                                   TCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAG  <  SRR19973067.3526535/150‑1 (MQ=60)
                                                                                                                                                   TCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAG  <  SRR19973067.3526417/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
ACAAACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAG  >  CP009273/2101763‑2102059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑