Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,383,897 C→A 89.3% R363L (CGC→CTC)  nuoN ← NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,383,8970CA89.3% 344.7 / 39.6 140R363L (CGC→CTC) nuoNNADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base C (8/7);  new base A (59/65);  total (67/73)
Fisher's exact test for biased strand distribution p-value = 7.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

Only 100 of 140 total aligned reads displayed.

CACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAG  >  CP009273/2383752‑2384039
                                                                                                                                                 |                                                                                                                                              
caccaGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCTGAACAGACCGCgg                                                                                                                                              >  2:2923278/1‑148 (MQ=255)
caccaGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAgg                                                                                                                                              <  1:627719/148‑1 (MQ=255)
caccaGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAgg                                                                                                                                              <  2:2500391/148‑1 (MQ=255)
caccaGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAgg                                                                                                                                              <  2:2502842/148‑1 (MQ=255)
  ccaGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTa                                                                                                                                            >  2:566794/1‑148 (MQ=255)
    aGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAag                                                                                                                                          <  2:1429829/148‑1 (MQ=255)
    aGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAag                                                                                                                                          >  2:103627/1‑148 (MQ=255)
     gCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAaga                                                                                                                                         >  1:1515562/1‑148 (MQ=255)
         ccacAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAAc                                                                                                                                     >  1:1501493/1‑148 (MQ=255)
          cacaAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACa                                                                                                                                    <  2:2394122/148‑1 (MQ=255)
               gtgtGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGaa                                                                                                                               <  2:1360344/148‑1 (MQ=255)
               gtgtGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGaa                                                                                                                               <  2:1360219/148‑1 (MQ=255)
                    ccTGGACACAGACCTCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCTGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGc                                                                                                                          <  1:1479065/148‑1 (MQ=255)
                     cTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCa                                                                                                                         <  2:1837597/148‑1 (MQ=255)
                       ggACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATc                                                                                                                       <  2:2421037/148‑1 (MQ=255)
                       ggACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATc                                                                                                                       <  2:2421020/148‑1 (MQ=255)
                        gACACCGACAGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATcc                                                                                                                      >  2:2323251/1‑148 (MQ=255)
                           accgaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCggg                                                                                                                   <  2:27057/148‑1 (MQ=255)
                           accgaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCggg                                                                                                                   <  2:2084893/148‑1 (MQ=255)
                              gaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCa                                                                                                                <  1:103627/148‑1 (MQ=255)
                                ccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACg                                                                                                              <  1:811312/148‑1 (MQ=255)
                                ccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACg                                                                                                              <  1:811025/148‑1 (MQ=255)
                                  gCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGAt                                                                                                            <  1:1896496/148‑1 (MQ=255)
                                  gCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGAt                                                                                                            <  2:3251473/148‑1 (MQ=255)
                                    cAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATAc                                                                                                          >  1:1758756/1‑148 (MQ=255)
                                       cACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACggg                                                                                                       >  1:2930354/1‑148 (MQ=255)
                                          gTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTg                                                                                                    <  1:1066144/148‑1 (MQ=255)
                                           tAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTgg                                                                                                   >  1:218300/1‑148 (MQ=255)
                                           tAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTgg                                                                                                   >  1:1788348/1‑148 (MQ=255)
                                            aGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGa                                                                                                  >  2:327521/1‑148 (MQ=255)
                                             gAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGAc                                                                                                 >  2:3173512/1‑148 (MQ=255)
                                               aCTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGATGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACAt                                                                                               <  2:2884789/148‑1 (MQ=255)
                                                cTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATc                                                                                              <  1:196540/148‑1 (MQ=255)
                                                cTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATc                                                                                              <  2:1534024/148‑1 (MQ=255)
                                                  tACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAg                                                                                            >  1:3200900/1‑148 (MQ=255)
                                                    ccGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGc                                                                                          <  2:595054/148‑1 (MQ=255)
                                                      gATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTg                                                                                        >  1:1971536/1‑148 (MQ=255)
                                                       aTAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGa                                                                                       >  1:508789/1‑148 (MQ=255)
                                                       aTAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGa                                                                                       >  1:2172398/1‑148 (MQ=255)
                                                       aTAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGa                                                                                       >  1:2171969/1‑148 (MQ=255)
                                                        tAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGAc                                                                                      >  2:2623860/1‑148 (MQ=255)
                                                           aGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCAc                                                                                   >  2:1285766/1‑148 (MQ=255)
                                                            gCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACg                                                                                  >  1:405917/1‑148 (MQ=255)
                                                              ccAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGcc                                                                                >  1:2505770/1‑148 (MQ=255)
                                                               cAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCg                                                                               >  1:1366438/1‑148 (MQ=255)
                                                                 gCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGaa                                                                             >  2:1635312/1‑148 (MQ=255)
                                                                  cGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAc                                                                            >  1:536551/1‑148 (MQ=255)
                                                                  cGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAc                                                                            >  2:3268325/1‑148 (MQ=255)
                                                                   gTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAcg                                                                           <  2:2029870/148‑1 (MQ=255)
                                                                   gTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAcg                                                                           <  2:2030175/148‑1 (MQ=255)
                                                                   gTCATCGGGAGACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAcg                                                                           <  2:962533/148‑1 (MQ=255)
                                                                    tCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAcgc                                                                          <  2:1738185/148‑1 (MQ=255)
                                                                     cATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAcgcg                                                                         >  1:1302414/1‑148 (MQ=255)
                                                                     cATCGGGATACCGGCCAGAGACAGCATCATAAACGTCATCACTGCCGCGAGGATCGGACGATGCATGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAcgcg                                                                         >  1:1302381/1‑148 (MQ=255)
                                                                      aTCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACgcgc                                                                        >  1:2377090/1‑148 (MQ=255)
                                                                      aTCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACgcgc                                                                        <  1:2332152/148‑1 (MQ=255)
                                                                        cGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCg                                                                      <  1:2776502/148‑1 (MQ=255)
                                                                           gATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAgg                                                                   <  1:2479596/148‑1 (MQ=255)
                                                                            aTACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGgc                                                                  <  2:1871335/148‑1 (MQ=255)
                                                                             tACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGgct                                                                 <  1:1386248/148‑1 (MQ=255)
                                                                              aCCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGgctg                                                                >  2:589745/1‑148 (MQ=255)
                                                                                  gCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGa                                                            >  2:1244246/1‑148 (MQ=255)
                                                                                  gCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGa                                                            >  2:1253236/1‑148 (MQ=255)
                                                                                   ccAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGaa                                                           <  1:1031497/148‑1 (MQ=255)
                                                                                      gagaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAg                                                        >  1:1207210/1‑148 (MQ=255)
                                                                                      gagaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAg                                                        <  2:2216477/148‑1 (MQ=255)
                                                                                      gagaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAg                                                        <  2:375267/148‑1 (MQ=255)
                                                                                       agaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGa                                                       >  1:1327114/1‑148 (MQ=255)
                                                                                        gaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGAt                                                      <  2:3057067/148‑1 (MQ=255)
                                                                                          cAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATaa                                                    <  1:724591/148‑1 (MQ=255)
                                                                                          cAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATaa                                                    >  2:159794/1‑148 (MQ=255)
                                                                                            gCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAAcc                                                  <  1:2414779/148‑1 (MQ=255)
                                                                                            gCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAAcc                                                  >  2:2779222/1‑148 (MQ=255)
                                                                                               tcatcaCCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGc                                               <  2:3064381/148‑1 (MQ=255)
                                                                                                catcaCCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGcc                                              >  1:953563/1‑148 (MQ=255)
                                                                                                catcaCCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGcc                                              >  1:952954/1‑148 (MQ=255)
                                                                                                         catcaCTGCCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAAcc                                     >  1:199084/1‑148 (MQ=255)
                                                                                                          atcaCTGCCGGGAGGATCGGACGATGCCATAACAGACAGTGGTAAGAGAACAGGGAATCTGCATCCGGGCCACGAGACGGGGTGGACATCCGTCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAAccc                                    <  1:2305528/148‑1 (MQ=255)
                                                                                                           tcaCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAAcccc                                   >  1:2133863/1‑148 (MQ=255)
                                                                                                            caCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCt                                  >  2:209832/1‑148 (MQ=255)
                                                                                                            caCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCt                                  >  2:341000/1‑148 (MQ=255)
                                                                                                            caCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCt                                  >  1:1913141/1‑148 (MQ=255)
                                                                                                             aCTGCCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTa                                 <  2:1565517/148‑1 (MQ=255)
                                                                                                             aCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTa                                 >  1:2448793/1‑148 (MQ=255)
                                                                                                             aCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTa                                 <  1:1844854/148‑1 (MQ=255)
                                                                                                              cTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTAc                                >  2:1049564/1‑148 (MQ=255)
                                                                                                                gCCGCGAGTATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCg                              <  1:2821054/148‑1 (MQ=255)
                                                                                                                 ccGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGc                             <  1:2623860/148‑1 (MQ=255)
                                                                                                                 ccGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGc                             >  2:2106020/1‑148 (MQ=255)
                                                                                                                     gAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc                         <  2:864217/148‑1 (MQ=255)
                                                                                                                     gAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc                         <  2:1441836/148‑1 (MQ=255)
                                                                                                                           cGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGAc                   <  2:1080127/148‑1 (MQ=255)
                                                                                                                           cGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGAc                   <  2:461654/148‑1 (MQ=255)
                                                                                                                              aCGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAtc                >  1:2226456/1‑148 (MQ=255)
                                                                                                                               cGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAtct               <  1:323727/148‑1 (MQ=255)
                                                                                                                                      aGAACAGACCGAGTTAAGAGAACAGGGAATCAGCATCCGGGCCACGATCCGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATACCCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGt        <  1:566794/148‑1 (MQ=255)
                                                                                                                                      aGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGt        <  2:2877347/148‑1 (MQ=255)
                                                                                                                                      aGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGt        <  2:1879034/148‑1 (MQ=255)
                                                                                                                                          cAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCTAACGCGCCGAGGCTGCTGAACATATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGc    <  1:1168067/148‑1 (MQ=255)
                                                                                                                                           aGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCa   <  1:2614574/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAG  >  CP009273/2383752‑2384039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGC  >  CP009273/2383749‑2384040
                                                                                                                                                    |                                                                                                                                               
ACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAG                                                                                                                                                >  SRR19973070.1061311/1‑150 (MQ=60)
ACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAG                                                                                                                                                >  SRR19973070.2724658/1‑150 (MQ=60)
ACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAG                                                                                                                                                <  SRR19973070.3123021/150‑1 (MQ=60)
   CACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTA                                                                                                                                             <  SRR19973070.632162/150‑1 (MQ=60)
      CAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGA                                                                                                                                          >  SRR19973070.1526182/1‑150 (MQ=60)
          CACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAAC                                                                                                                                      >  SRR19973070.3164007/1‑150 (MQ=60)
          CACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAAC                                                                                                                                      >  SRR19973070.1512007/1‑150 (MQ=60)
           ACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACA                                                                                                                                     >  SRR19973070.2365097/1‑150 (MQ=60)
                       CCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCAT                                                                                                                         <  SRR19973070.1489187/150‑1 (MQ=60)
                       CCTGGACACAGACCTCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCTGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCAT                                                                                                                         <  SRR19973070.1489416/150‑1 (MQ=60)
                         TGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTACTCGGGATACATGCCCGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCC                                                                                                                       >  SRR19973070.3270342/1‑150 (MQ=60)
                              ACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCC                                                                                                                  >  SRR19973070.126664/1‑150 (MQ=60)
                                 GACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACG                                                                                                               <  SRR19973070.104608/150‑1 (MQ=60)
                                   CCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGAT                                                                                                             <  SRR19973070.816582/150‑1 (MQ=60)
                                   CCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGAT                                                                                                             <  SRR19973070.816872/150‑1 (MQ=60)
                                     GCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATAC                                                                                                           <  SRR19973070.1909347/150‑1 (MQ=60)
                                     GCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATAC                                                                                                           >  SRR19973070.1770934/1‑150 (MQ=60)
                                        AGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGG                                                                                                        >  SRR19973070.2949260/1‑150 (MQ=60)
                                          CACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCT                                                                                                      >  SRR19973070.1936719/1‑150 (MQ=60)
                                            CGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGG                                                                                                    >  SRR19973070.219886/1‑150 (MQ=60)
                                            CGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGG                                                                                                    >  SRR19973070.1800709/1‑150 (MQ=60)
                                             GTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGA                                                                                                   <  SRR19973070.1073611/150‑1 (MQ=60)
                                                   CTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAG                                                                                             >  SRR19973070.3221611/1‑150 (MQ=60)
                                                   CTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAG                                                                                             <  SRR19973070.198041/150‑1 (MQ=60)
                                                   CTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAAAAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAG                                                                                             >  SRR19973070.1764989/1‑150 (MQ=60)
                                                       CCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTG                                                                                         >  SRR19973070.1984859/1‑150 (MQ=60)
                                                        CGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGA                                                                                        >  SRR19973070.2186370/1‑150 (MQ=60)
                                                        CGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGA                                                                                        >  SRR19973070.2186376/1‑150 (MQ=60)
                                                        CGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGA                                                                                        >  SRR19973070.2186801/1‑150 (MQ=60)
                                                        CGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGA                                                                                        >  SRR19973070.512301/1‑150 (MQ=60)
                                                             AAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACG                                                                                   >  SRR19973070.408896/1‑150 (MQ=60)
                                                               GCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCC                                                                                 >  SRR19973070.2522049/1‑150 (MQ=60)
                                                                CCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCG                                                                                >  SRR19973070.1375744/1‑150 (MQ=60)
                                                                   AGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAC                                                                             >  SRR19973070.540149/1‑150 (MQ=60)
                                                                      GTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCG                                                                          >  SRR19973070.2622424/1‑150 (MQ=60)
                                                                      GTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCG                                                                          >  SRR19973070.1311469/1‑150 (MQ=60)
                                                                      GTCATCGGGATACCGGCCAGAGACAGCATCATAAACGTCATCACTGCCGCGAGGATCGGACGATGCATGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCG                                                                          >  SRR19973070.1311436/1‑150 (MQ=60)
                                                                       TCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGC                                                                         >  SRR19973070.2392799/1‑150 (MQ=60)
                                                                         ATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCG                                                                       <  SRR19973070.2347639/150‑1 (MQ=60)
                                                                           CGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAG                                                                     <  SRR19973070.2794327/150‑1 (MQ=60)
                                                                              GATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCT                                                                  <  SRR19973070.2495737/150‑1 (MQ=60)
                                                                                TACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGC                                                                <  SRR19973070.1395624/150‑1 (MQ=60)
                                                                                      CCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACA                                                          <  SRR19973070.1038795/150‑1 (MQ=60)
                                                                                       CAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAG                                                         >  SRR19973070.1215689/1‑150 (MQ=60)
                                                                                        AGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGA                                                        >  SRR19973070.1336273/1‑150 (MQ=60)
                                                                                            ACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAAC                                                    <  SRR19973070.834320/150‑1 (MQ=60)
                                                                                             CAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACC                                                   <  SRR19973070.729735/150‑1 (MQ=60)
                                                                                              AGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCG                                                  <  SRR19973070.1368154/150‑1 (MQ=60)
                                                                                               GCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGG                                                 <  SRR19973070.2430676/150‑1 (MQ=60)
                                                                                               GCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGG                                                 <  SRR19973070.2430699/150‑1 (MQ=60)
                                                                                                 ATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCC                                               >  SRR19973070.960371/1‑150 (MQ=60)
                                                                                                 ATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCC                                               >  SRR19973070.959757/1‑150 (MQ=60)
                                                                                                   CATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAG                                             >  SRR19973070.1987655/1‑150 (MQ=60)
                                                                                                          GTCATCACTGCCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACC                                      >  SRR19973070.200598/1‑150 (MQ=60)
                                                                                                            CATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCC                                    >  SRR19973070.1856475/1‑150 (MQ=60)
                                                                                                            CATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCC                                    >  SRR19973070.2148122/1‑150 (MQ=60)
                                                                                                             ATCACTGCCGGGAGGATCGGACGATGCCATAACAGACAGTGGTAAGAGAACAGGGAATCTGCATCCGGGCCACGAGACGGGGTGGACATCCGTCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCT                                   <  SRR19973070.2320860/150‑1 (MQ=60)
                                                                                                             ATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCT                                   >  SRR19973070.1926065/1‑150 (MQ=60)
                                                                                                             ATCACTGCCGCGAGGATCGGACGAGGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCT                                   <  SRR19973070.648919/150‑1 (MQ=60)
                                                                                                              TCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTA                                  >  SRR19973070.2464812/1‑150 (MQ=60)
                                                                                                                ACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACC                                <  SRR19973070.1857506/150‑1 (MQ=60)
                                                                                                                   GCCGCGAGTATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCT                             <  SRR19973070.2839292/150‑1 (MQ=60)
                                                                                                                   GCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCT                             <  SRR19973070.2656234/150‑1 (MQ=60)
                                                                                                                    CCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTT                            <  SRR19973070.2640981/150‑1 (MQ=60)
                                                                                                                         AGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATC                       >  SRR19973070.2235460/1‑150 (MQ=60)
                                                                                                                               GGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATC                 >  SRR19973070.560154/1‑150 (MQ=60)
                                                                                                                               GGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATC                 >  SRR19973070.2241095/1‑150 (MQ=60)
                                                                                                                                  CGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCG              <  SRR19973070.326079/150‑1 (MQ=60)
                                                                                                                                         AGAACAGACCGAGTTAAGAGAACAGGGAATCAGCATCCGGGCCACGATCCGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATACCCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTT       <  SRR19973070.570602/150‑1 (MQ=60)
                                                                                                                                             CAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCTAACGCGCCGAGGCTGCTGAACATATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAG   <  SRR19973070.1176244/150‑1 (MQ=60)
                                                                                                                                             CAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAG   <  SRR19973070.1175711/150‑1 (MQ=60)
                                                                                                                                              AGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGC  <  SRR19973070.2631633/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                               
ACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGC  >  CP009273/2383749‑2384040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑