Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,383,897 C→A 100% R363L (CGC→CTC)  nuoN ← NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,383,8970CA97.4% 469.1 / 3.9 152R363L (CGC→CTC) nuoNNADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (73/73);  total (75/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 152 total aligned reads displayed.

CACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTT  >  CP009273/2383752‑2384035
                                                                                                                                                 |                                                                                                                                          
caccaGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCTTCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAgg                                                                                                                                          <  1:41939/148‑1 (MQ=255)
      ccaccaCAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAagag                                                                                                                                    <  2:803053/148‑1 (MQ=255)
       caccacAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAgaga                                                                                                                                   <  1:1505145/148‑1 (MQ=255)
          cacaAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACa                                                                                                                                <  2:1705132/148‑1 (MQ=255)
              aGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTAATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGa                                                                                                                            >  1:3474140/1‑148 (MQ=255)
                tgtgCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAAt                                                                                                                          <  1:3059719/148‑1 (MQ=255)
                     cTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCa                                                                                                                     <  2:319072/148‑1 (MQ=255)
                      tGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCAt                                                                                                                    >  2:1359330/1‑148 (MQ=255)
                      tGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCAt                                                                                                                    >  2:1359329/1‑148 (MQ=255)
                        gACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATcc                                                                                                                  >  2:455422/1‑148 (MQ=255)
                        gACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATcc                                                                                                                  >  1:2163782/1‑148 (MQ=255)
                          cacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCgg                                                                                                                >  2:3208303/1‑148 (MQ=255)
                          cacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCgg                                                                                                                >  2:2307770/1‑148 (MQ=255)
                                ccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACg                                                                                                          >  1:2118890/1‑148 (MQ=255)
                                  gCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGAt                                                                                                        <  2:2802435/148‑1 (MQ=255)
                                      gCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACgg                                                                                                    <  1:1179287/148‑1 (MQ=255)
                                       cACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACggg                                                                                                   >  2:691417/1‑148 (MQ=255)
                                       cACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACggg                                                                                                   >  2:691426/1‑148 (MQ=255)
                                        aCGTAGAACTTACCGATACAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGc                                                                                                  <  2:1885161/148‑1 (MQ=255)
                                          gTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTg                                                                                                <  1:2996232/148‑1 (MQ=255)
                                           tAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTgg                                                                                               >  2:2639804/1‑148 (MQ=255)
                                            aGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAggg                                                                                                                             >  1:363530/1‑117 (MQ=255)
                                            aGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAggg                                                                                                                             <  2:363530/117‑1 (MQ=255)
                                               aCTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACAt                                                                                           >  1:2023590/1‑148 (MQ=255)
                                               aCTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACAt                                                                                           >  1:1680173/1‑148 (MQ=255)
                                                cTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATc                                                                                          >  1:2110583/1‑148 (MQ=255)
                                                 ttACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCa                                                                                         <  1:828259/148‑1 (MQ=255)
                                                  tACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAg                                                                                        >  1:438724/1‑148 (MQ=255)
                                                   aCCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAgg                                                                                       <  2:420350/148‑1 (MQ=255)
                                                     cGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCt                                                                                     >  1:2737821/1‑148 (MQ=255)
                                                     cGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCt                                                                                     <  2:1543544/148‑1 (MQ=255)
                                                          aaGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCa                                                                                >  2:3121243/1‑148 (MQ=255)
                                                           aGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCAc                                                                               >  1:1461334/1‑148 (MQ=255)
                                                            gCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACg                                                                              <  1:1201577/148‑1 (MQ=255)
                                                             cccAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGc                                                                             >  2:200097/1‑148 (MQ=255)
                                                              ccAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGcc                                                                            <  2:2001476/148‑1 (MQ=255)
                                                              ccAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGcc                                                                            >  2:2035162/1‑148 (MQ=255)
                                                              ccAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGcc                                                                            >  2:2035659/1‑148 (MQ=255)
                                                               cAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGAGGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCg                                                                           <  2:2564924/148‑1 (MQ=255)
                                                                aGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGa                                                                          <  2:2348494/148‑1 (MQ=255)
                                                                aGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGa                                                                          <  1:2395819/148‑1 (MQ=255)
                                                                 gCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGaa                                                                         <  1:2035000/148‑1 (MQ=255)
                                                                  cGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAc                                                                        >  1:993706/1‑148 (MQ=255)
                                                                    tCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAcgc                                                                      >  1:2074988/1‑148 (MQ=255)
                                                                     cATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAcgcg                                                                     <  2:2113658/148‑1 (MQ=255)
                                                                     cATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAcgcg                                                                     >  1:1606836/1‑148 (MQ=255)
                                                                         gggATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACTATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGa                                                                 <  2:1618911/148‑1 (MQ=255)
                                                                            aTACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGgc                                                              <  2:170771/148‑1 (MQ=255)
                                                                            aTACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGgc                                                              <  2:170670/148‑1 (MQ=255)
                                                                              aCCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGTGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGCCCACGCCGACCGCGCCGAGgctg                                                            >  2:3246383/1‑148 (MQ=255)
                                                                              aCCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGgctg                                                            >  1:126527/1‑148 (MQ=255)
                                                                               ccGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGgctgc                                                           <  1:2639804/148‑1 (MQ=255)
                                                                                  gCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGa                                                        >  2:278903/1‑148 (MQ=255)
                                                                                  gCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGa                                                        >  2:1858194/1‑148 (MQ=255)
                                                                                  gCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGa                                                        >  2:1105029/1‑148 (MQ=255)
                                                                                      gagaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAg                                                    <  1:3208276/148‑1 (MQ=255)
                                                                                      gagaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAg                                                    <  1:3208303/148‑1 (MQ=255)
                                                                                      gagaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAg                                                    <  1:2307770/148‑1 (MQ=255)
                                                                                       agaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGa                                                   <  1:2760385/148‑1 (MQ=255)
                                                                                       agaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGa                                                   <  2:3119359/148‑1 (MQ=255)
                                                                                       agaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGa                                                   <  1:1037423/148‑1 (MQ=255)
                                                                                       agaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGa                                                   <  2:1981256/148‑1 (MQ=255)
                                                                                       agaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGa                                                   <  2:3187512/148‑1 (MQ=255)
                                                                                       agaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGGAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGa                                                   <  2:1981238/148‑1 (MQ=255)
                                                                                        gaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGAt                                                  <  2:1302499/148‑1 (MQ=255)
                                                                                         aCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATa                                                 >  1:2640429/1‑148 (MQ=255)
                                                                                         aCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATa                                                 <  1:1359330/148‑1 (MQ=255)
                                                                                         aCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATa                                                 <  1:1359329/148‑1 (MQ=255)
                                                                                         aCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATa                                                 >  1:1941329/1‑148 (MQ=255)
                                                                                            gCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAAcc                                              <  1:1659677/148‑1 (MQ=255)
                                                                                             catcatCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCg                                             >  2:3088950/1‑148 (MQ=255)
                                                                                             catcatCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCg                                             >  2:3450432/1‑148 (MQ=255)
                                                                                             catcatCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCg                                             >  1:65910/1‑148 (MQ=255)
                                                                                             catcatCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCg                                             >  1:833664/1‑148 (MQ=255)
                                                                                                 atcaCCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCa                                         >  2:2580900/1‑148 (MQ=255)
                                                                                                   caCCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAgg                                       >  2:302555/1‑148 (MQ=255)
                                                                                                   caCCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAgg                                       <  2:2438909/148‑1 (MQ=255)
                                                                                                    aCCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGt                                      >  1:823625/1‑148 (MQ=255)
                                                                                                      cGTCATCACTGCCGCGAGGATCGGTCGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTaa                                    <  2:1025383/148‑1 (MQ=255)
                                                                                                       gTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTaaa                                   <  2:2070790/148‑1 (MQ=255)
                                                                                                       gTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTaaa                                   <  2:765611/148‑1 (MQ=255)
                                                                                                       gTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTaaa                                   <  2:710066/148‑1 (MQ=255)
                                                                                                          atcaCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAAccc                                >  1:1688428/1‑148 (MQ=255)
                                                                                                            caCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACTATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCt                              >  2:828933/1‑148 (MQ=255)
                                                                                                            caCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCt                              >  2:1708990/1‑148 (MQ=255)
                                                                                                            caCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCt                              >  1:3521988/1‑148 (MQ=255)
                                                                                                              cTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTAc                            >  1:2964561/1‑148 (MQ=255)
                                                                                                               tGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTAcc                           >  2:430057/1‑148 (MQ=255)
                                                                                                                 ccGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGc                         >  1:2514870/1‑148 (MQ=255)
                                                                                                                   gcgAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCtt                       >  2:1727592/1‑148 (MQ=255)
                                                                                                                   gcgAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCtt                       >  2:125438/1‑148 (MQ=255)
                                                                                                                    cgAGGATCGGACGATGCCAGACCAGACCGTGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATACCCGGCCAGGTAAACCCCTACCGCTTc                      <  1:3342477/148‑1 (MQ=255)
                                                                                                                          tCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGa                <  2:2374887/148‑1 (MQ=255)
                                                                                                                            ggACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACa              <  1:1647924/148‑1 (MQ=255)
                                                                                                                            ggACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACa              <  1:24817/148‑1 (MQ=255)
                                                                                                                              aCGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAtc            <  1:1601367/148‑1 (MQ=255)
                                                                                                                                 aTGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCg         <  2:3360320/148‑1 (MQ=255)
                                                                                                                                  tGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGc        <  2:821298/148‑1 (MQ=255)
                                                                                                                                       gAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGtt   >  1:2973177/1‑148 (MQ=255)
                                                                                                                                        aaCAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGttt  >  2:1412954/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                          
CACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTT  >  CP009273/2383752‑2384035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTT  >  CP009273/2383748‑2384034
                                                                                                                                                     |                                                                                                                                         
CACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGA                                                                                                                                           >  SRR19973067.1057016/1‑150 (MQ=60)
 ACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAG                                                                                                                                          >  SRR19973067.2454928/1‑150 (MQ=60)
    CACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCTTCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTA                                                                                                                                       <  SRR19973067.42525/150‑1 (MQ=60)
           CACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAAC                                                                                                                                <  SRR19973067.1515085/150‑1 (MQ=60)
           CACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAAC                                                                                                                                <  SRR19973067.1515082/150‑1 (MQ=60)
                CAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTAATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGA                                                                                                                           >  SRR19973067.3496205/1‑150 (MQ=60)
                    TGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCA                                                                                                                       <  SRR19973067.3079199/150‑1 (MQ=60)
                          TGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCC                                                                                                                 >  SRR19973067.2178249/1‑150 (MQ=60)
                                  GACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACG                                                                                                         >  SRR19973067.2133020/1‑150 (MQ=60)
                                          GCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGC                                                                                                 <  SRR19973067.1187078/150‑1 (MQ=60)
                                          GCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGC                                                                                                 <  SRR19973067.1187240/150‑1 (MQ=60)
                                           CACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCT                                                                                                >  SRR19973067.1800013/1‑150 (MQ=60)
                                              GTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAAagatcggaagagcacacgtctgaactcca                                                                                             >  SRR19973067.366056/1‑121 (MQ=60)
                                              GTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGA                                                                                             <  SRR19973067.3015226/150‑1 (MQ=60)
                                                AGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACA                                                                                           >  SRR19973067.1453658/1‑150 (MQ=60)
                                                 GAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACAT                                                                                          >  SRR19973067.1691750/1‑150 (MQ=60)
                                                 GAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACAT                                                                                          >  SRR19973067.1691700/1‑150 (MQ=60)
                                                 GAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACAT                                                                                          >  SRR19973067.2037171/1‑150 (MQ=60)
                                                  AACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATC                                                                                         >  SRR19973067.2124616/1‑150 (MQ=60)
                                                    CTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAG                                                                                       >  SRR19973067.441777/1‑150 (MQ=60)
                                                     TTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGG                                                                                      <  SRR19973067.833908/150‑1 (MQ=60)
                                                       ACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCT                                                                                    >  SRR19973067.2755505/1‑150 (MQ=60)
                                                          GATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGAC                                                                                 >  SRR19973067.3207660/1‑150 (MQ=60)
                                                             AAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCAC                                                                              >  SRR19973067.1471095/1‑150 (MQ=60)
                                                             AAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCAC                                                                              <  SRR19973067.250292/150‑1 (MQ=60)
                                                              AAGCCCAGCGTCATCGGGATACCGGACAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACG                                                                             >  SRR19973067.2710347/1‑150 (MQ=60)
                                                                GCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCC                                                                           <  SRR19973067.1209432/150‑1 (MQ=60)
                                                                GCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATAAGGCTGACCACGCC                                                                           <  SRR19973067.1341029/150‑1 (MQ=60)
                                                                    AGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAC                                                                       >  SRR19973067.1000545/1‑150 (MQ=60)
                                                                    AGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAC                                                                       <  SRR19973067.2411423/150‑1 (MQ=60)
                                                                    AGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAC                                                                       <  SRR19973067.2411368/150‑1 (MQ=60)
                                                                     GCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACG                                                                      <  SRR19973067.2048630/150‑1 (MQ=60)
                                                                      CGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGC                                                                     >  SRR19973067.2088763/1‑150 (MQ=60)
                                                                       GTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCG                                                                    >  SRR19973067.1617867/1‑150 (MQ=60)
                                                                       GTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCG                                                                    >  SRR19973067.1617902/1‑150 (MQ=60)
                                                                                ATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTG                                                           >  SRR19973067.127691/1‑150 (MQ=60)
                                                                                   CCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTG                                                        <  SRR19973067.2656866/150‑1 (MQ=60)
                                                                                         AGAGATAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGA                                                  >  SRR19973067.3543903/1‑150 (MQ=60)
                                                                                          GAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGAT                                                 <  SRR19973067.2322976/150‑1 (MQ=60)
                                                                                          GAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGAT                                                 <  SRR19973067.3228520/150‑1 (MQ=60)
                                                                                          GAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGAT                                                 <  SRR19973067.3228493/150‑1 (MQ=60)
                                                                                           AGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATA                                                <  SRR19973067.457515/150‑1 (MQ=60)
                                                                                           AGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATA                                                <  SRR19973067.2778226/150‑1 (MQ=60)
                                                                                           AGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATA                                                <  SRR19973067.1044562/150‑1 (MQ=60)
                                                                                           AGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATA                                                >  SRR19973067.2657492/1‑150 (MQ=60)
                                                                                           AGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATA                                                >  SRR19973067.2088919/1‑150 (MQ=60)
                                                                                           AGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATA                                                >  SRR19973067.1954567/1‑150 (MQ=60)
                                                                                             ACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAAC                                              <  SRR19973067.1368613/150‑1 (MQ=60)
                                                                                             ACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAAC                                              <  SRR19973067.1368614/150‑1 (MQ=60)
                                                                                               AGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCG                                            >  SRR19973067.839336/1‑150 (MQ=60)
                                                                                               AGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCG                                            >  SRR19973067.66693/1‑150 (MQ=60)
                                                                                                GCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGG                                           <  SRR19973067.1671123/150‑1 (MQ=60)
                                                                                                 CATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGC                                          >  SRR19973067.3250593/1‑150 (MQ=60)
                                                                                                      TCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGT                                     >  SRR19973067.2582683/1‑150 (MQ=60)
                                                                                                      TCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGT                                     >  SRR19973067.829339/1‑150 (MQ=60)
                                                                                                      TCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGT                                     >  SRR19973067.829254/1‑150 (MQ=60)
                                                                                                        ACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAA                                   <  SRR19973067.391780/150‑1 (MQ=60)
                                                                                                           GTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACC                                >  SRR19973067.2155999/1‑150 (MQ=60)
                                                                                                            TCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCC                               >  SRR19973067.1700058/1‑150 (MQ=60)
                                                                                                              ATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCT                             >  SRR19973067.3544263/1‑150 (MQ=60)
                                                                                                                CACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTAC                           >  SRR19973067.2983284/1‑150 (MQ=60)
                                                                                                                  CTGCCGCGAGGATCGGACGATGCAAGAAAATACCTAGGTAAGAGAACAGGTAATCAGCATCCGGGCCACTATACGGGCTGGAAATCTGGCTGACCACGCCGAACGCGCCGTGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCG                         <  SRR19973067.2824018/150‑1 (MQ=60)
                                                                                                                   TGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGC                        >  SRR19973067.2531362/1‑150 (MQ=60)
                                                                                                                        CGAGGATCGGACGATGCCAGACCAGACCGTGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATACCCGGCCAGGTAAACCCCTACCGCTTCCA                   <  SRR19973067.3363653/150‑1 (MQ=60)
                                                                                                                        CGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCA                   <  SRR19973067.204197/150‑1 (MQ=60)
                                                                                                                        CGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCA                   <  SRR19973067.3008270/150‑1 (MQ=60)
                                                                                                                                GGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATC           <  SRR19973067.1659273/150‑1 (MQ=60)
                                                                                                                                GGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATC           <  SRR19973067.25203/150‑1 (MQ=60)
                                                                                                                                  ACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTC         <  SRR19973067.1612360/150‑1 (MQ=60)
                                                                                                                                     ATGCAAGAACAGACCGTTGTAAGAGAACAGGAAATCAGCAACCGGGCCACTATACGGGATGGAAATCAGGCTGACCACGCCGAACGCGCCGTGGCTGATGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCC      <  SRR19973067.908601/150‑1 (MQ=60)
                                                                                                                                         CAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTT  >  SRR19973067.2991924/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                         
CACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTT  >  CP009273/2383748‑2384034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑