Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,488,416 G→T 86.4% P80P (CCG→CCT)  ypdI → putative lipoprotein involved in colanic acid biosynthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,488,4160GT86.4% 477.3 / 76.4 211P80P (CCG→CCT) ypdIputative lipoprotein involved in colanic acid biosynthesis
Reads supporting (aligned to +/- strand):  ref base G (11/18);  new base T (81/101);  total (92/119)
Fisher's exact test for biased strand distribution p-value = 5.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 211 total aligned reads displayed.

AGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATC  >  CP009273/2488271‑2488562
                                                                                                                                                 |                                                                                                                                                  
agagGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAATATGAGACAACATGCGCCACCATTAAACCTCACACAAGAAAACCCCCCCCCATTTCCTAc                                                                                                                                                  >  1:3179122/1‑148 (MQ=255)
 gaggagCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACa                                                                                                                                                 >  2:1178118/1‑148 (MQ=255)
 gaggagCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACa                                                                                                                                                 <  2:1114591/148‑1 (MQ=255)
 gaggagCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACa                                                                                                                                                 >  1:1759949/1‑148 (MQ=255)
  aggagCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACaa                                                                                                                                                <  2:1404001/148‑1 (MQ=255)
   ggagCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACaaa                                                                                                                                               <  2:1604878/148‑1 (MQ=255)
   ggagCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACaaa                                                                                                                                               <  1:1655908/148‑1 (MQ=255)
    gagCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAg                                                                                                                                              <  2:1623052/148‑1 (MQ=255)
        tataAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGa                                                                                                                                          <  2:2037756/148‑1 (MQ=255)
           aaaGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAATATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAAtt                                                                                                                                       >  1:506993/1‑148 (MQ=255)
           aaaGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACCTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAAtt                                                                                                                                       <  1:1252902/148‑1 (MQ=255)
             agagACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTGATATGAGAGAAGATGCGCCACCATTAAACCTCCCAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGACTTAt                                                                                                                                     >  1:1953721/1‑148 (MQ=255)
             agagACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTAt                                                                                                                                     >  2:302158/1‑148 (MQ=255)
              gagaCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCCCCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGCATTATc                                                                                                                                    >  1:36682/1‑148 (MQ=255)
              gagaCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATc                                                                                                                                    <  1:636970/148‑1 (MQ=255)
               agaCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAATATGCGCCACCATTAAAACTCACATAAGAAATGCCGCCCCCTTATCCGACAAAGGCGAATTATCt                                                                                                                                   >  1:1875149/1‑148 (MQ=255)
               agaCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCt                                                                                                                                   <  2:2626995/148‑1 (MQ=255)
                gaCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCtt                                                                                                                                  <  2:811494/148‑1 (MQ=255)
                 aCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTa                                                                                                                                 <  2:719680/148‑1 (MQ=255)
                   tGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTAtt                                                                                                                               >  2:652822/1‑148 (MQ=255)
                   tGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTAtt                                                                                                                               >  2:837964/1‑148 (MQ=255)
                   tGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTAtt                                                                                                                               >  2:2494835/1‑148 (MQ=255)
                   tGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCACACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTAtt                                                                                                                               >  2:2563802/1‑148 (MQ=255)
                    gAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTc                                                                                                                              <  2:1237734/148‑1 (MQ=255)
                    gAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTc                                                                                                                              <  1:2155946/148‑1 (MQ=255)
                    gAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTc                                                                                                                              <  2:1267398/148‑1 (MQ=255)
                         aaCATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCt                                                                                                                         >  2:2178456/1‑148 (MQ=255)
                          aCATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCtg                                                                                                                        >  2:3241604/1‑148 (MQ=255)
                           cATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCtgt                                                                                                                       >  2:2541736/1‑148 (MQ=255)
                            aTCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCtgtg                                                                                                                      <  2:238255/148‑1 (MQ=255)
                              cAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCg                                                                                                                    <  2:975148/148‑1 (MQ=255)
                                gCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGAt                                                                                                                  <  2:2464471/148‑1 (MQ=255)
                                 cGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATa                                                                                                                 <  1:1178118/148‑1 (MQ=255)
                                    tGACGACCACACACTCTACAAACCTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCACCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCg                                                                                                              <  1:3057437/148‑1 (MQ=255)
                                    tGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGACGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCg                                                                                                              <  1:2680235/148‑1 (MQ=255)
                                    tGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCg                                                                                                              <  1:2227250/148‑1 (MQ=255)
                                     gacgacCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGt                                                                                                             <  1:3241604/148‑1 (MQ=255)
                                      acgacCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTc                                                                                                            <  1:3294504/148‑1 (MQ=255)
                                         acCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCttt                                                                                                         >  2:3138171/1‑148 (MQ=255)
                                           cacacaCTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTca                                                                                                       >  2:2217724/1‑148 (MQ=255)
                                           cacacaCTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTca                                                                                                       <  2:1507564/148‑1 (MQ=255)
                                            acacacTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTcac                                                                                                      <  1:2494835/148‑1 (MQ=255)
                                            acacacTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTcac                                                                                                      <  1:1465500/148‑1 (MQ=255)
                                             cacacTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTcaca                                                                                                     >  2:2240858/1‑148 (MQ=255)
                                             cacacTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTcaca                                                                                                     >  1:2433247/1‑148 (MQ=255)
                                                 ctctACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGTCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGc                                                                                                 <  2:1200020/148‑1 (MQ=255)
                                                  tctACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCa                                                                                                <  1:2912447/148‑1 (MQ=255)
                                                  tctACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCa                                                                                                <  2:2357686/148‑1 (MQ=255)
                                                  tctACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCa                                                                                                <  1:2912482/148‑1 (MQ=255)
                                                   ctACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAg                                                                                               <  1:896156/148‑1 (MQ=255)
                                                    tACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAgg                                                                                              <  1:2092052/148‑1 (MQ=255)
                                                       aaaaCTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGa                                                                                           <  2:2448154/148‑1 (MQ=255)
                                                        aaaCTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGaa                                                                                          <  2:324496/148‑1 (MQ=255)
                                                          aCTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACa                                                                                        <  1:2254383/148‑1 (MQ=255)
                                                              cAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCa                                                                                    <  2:1484382/148‑1 (MQ=255)
                                                              cAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCa                                                                                    <  1:42143/148‑1 (MQ=255)
                                                              cAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCa                                                                                    <  1:2444826/148‑1 (MQ=255)
                                                               aGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCAt                                                                                   <  1:2953345/148‑1 (MQ=255)
                                                                 ccATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGt                                                                                 <  1:2254928/148‑1 (MQ=255)
                                                                    tCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTctg                                                                              <  2:2655627/148‑1 (MQ=255)
                                                                       caGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTctgatg                                                                           <  2:2885987/148‑1 (MQ=255)
                                                                        aGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTctgatgc                                                                          <  2:1921437/148‑1 (MQ=255)
                                                                              ttttGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGAtgt                                                                    >  1:1438494/1‑148 (MQ=255)
                                                                              ttttGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGAtgt                                                                    <  1:1417686/148‑1 (MQ=255)
                                                                               tttGTTTAAGATGAGAGAAGATGCGCCACCATTCAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCGTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGAGGCTGAtgtg                                                                   >  2:594131/1‑148 (MQ=255)
                                                                               tttGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGAtgtg                                                                   >  2:2709469/1‑148 (MQ=255)
                                                                               tttGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGAtgtg                                                                   >  2:2710331/1‑148 (MQ=255)
                                                                               tttGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGAtgtg                                                                   >  2:2742396/1‑148 (MQ=255)
                                                                                 tGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGaa                                                                 <  1:107674/148‑1 (MQ=255)
                                                                                    ttAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAacac                                                              >  1:1362243/1‑148 (MQ=255)
                                                                                    ttAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAacac                                                              >  1:3010474/1‑148 (MQ=255)
                                                                                     tAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACAcc                                                             >  1:2720353/1‑148 (MQ=255)
                                                                                       aGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTg                                                           <  1:2541736/148‑1 (MQ=255)
                                                                                            agagaAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCaa                                                      <  2:1408560/148‑1 (MQ=255)
                                                                                             gagaAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCaaa                                                     <  1:752571/148‑1 (MQ=255)
                                                                                             gagaAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCaaa                                                     <  2:564493/148‑1 (MQ=255)
                                                                                                 agaTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAg                                                 >  1:1194404/1‑148 (MQ=255)
                                                                                                  gaTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGc                                                >  2:2831548/1‑148 (MQ=255)
                                                                                                  gaTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGc                                                >  2:2589990/1‑148 (MQ=255)
                                                                                                   aTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGcc                                               >  2:2942360/1‑148 (MQ=255)
                                                                                                   aTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGcc                                               <  2:2676051/148‑1 (MQ=255)
                                                                                                      cgcCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATc                                            <  1:289749/148‑1 (MQ=255)
                                                                                                         caccaTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTg                                         <  2:506993/148‑1 (MQ=255)
                                                                                                         caccaTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGACATCCTg                                         >  2:1957039/1‑148 (MQ=255)
                                                                                                            caTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGAcc                                      >  1:2568249/1‑148 (MQ=255)
                                                                                                              ttAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCa                                    <  2:3178104/148‑1 (MQ=255)
                                                                                                              ttAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCa                                    >  1:2467096/1‑148 (MQ=255)
                                                                                                              ttAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCa                                    >  1:1002320/1‑148 (MQ=255)
                                                                                                              ttAAACCTCACAGAAGAAAGGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTTAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCa                                    <  2:3179122/148‑1 (MQ=255)
                                                                                                                  aCCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTAc                                >  1:2404260/1‑148 (MQ=255)
                                                                                                                     tCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGc                             <  2:960255/148‑1 (MQ=255)
                                                                                                                        caGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTg                          >  1:1983644/1‑148 (MQ=255)
                                                                                                                         agaagaACTGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATACATCCTGTGCGATAGCGTCTTTCACATGGCATGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGc                         <  2:1875149/148‑1 (MQ=255)
                                                                                                                           aagaaATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCa                       >  1:329037/1‑148 (MQ=255)
                                                                                                                           aagaaATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCa                       >  1:1202933/1‑148 (MQ=255)
                                                                                                                             gaaATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATa                     >  1:2886325/1‑148 (MQ=255)
                                                                                                                             gaaATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATa                     <  2:1953721/148‑1 (MQ=255)
                                                                                                                              aaaTGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATAc                    >  2:2288040/1‑148 (MQ=255)
                                                                                                                                            tttCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCg      >  1:1966557/1‑148 (MQ=255)
                                                                                                                                            tttCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCg      >  2:1078223/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
AGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATC  >  CP009273/2488271‑2488562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATC  >  CP009273/2488267‑2488565
                                                                                                                                                     |                                                                                                                                                     
AGTGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCT                                                                                                                                                       <  SRR19973070.1638677/150‑1 (MQ=60)
AGTGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCACACAGAAGAAATGCCGCCCCCTTTTCCT                                                                                                                                                       >  SRR19973070.2672902/1‑150 (MQ=60)
  TGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATTCGCCACCATTAAACCTCACATAAGAAATTCCGCCCCCTTTTCCTAC                                                                                                                                                     >  SRR19973070.3198681/1‑150 (MQ=60)
   GAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACA                                                                                                                                                    >  SRR19973070.1772133/1‑150 (MQ=60)
      AGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAG                                                                                                                                                 <  SRR19973070.1317439/150‑1 (MQ=60)
       GGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGG                                                                                                                                                <  SRR19973070.1667318/150‑1 (MQ=60)
       GGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGG                                                                                                                                                <  SRR19973070.1667319/150‑1 (MQ=60)
         AGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTATACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCG                                                                                                                                              <  SRR19973070.1394424/150‑1 (MQ=60)
             ATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAATATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATT                                                                                                                                          >  SRR19973070.510495/1‑150 (MQ=60)
              TAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTA                                                                                                                                         <  SRR19973070.2249049/150‑1 (MQ=60)
               AAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTGATATGAGAGAAGATGCGCCACCATTAAACCTCCCAGAAGAAATGCCGCCCCCATTTCCGACAAAGGCGACTTAT                                                                                                                                        >  SRR19973070.1966865/1‑150 (MQ=60)
               AAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTAT                                                                                                                                        <  SRR19973070.3169270/150‑1 (MQ=60)
               AAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACCTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTAT                                                                                                                                        <  SRR19973070.1261688/150‑1 (MQ=60)
                AAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTTTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGTATTATC                                                                                                                                       >  SRR19973070.37435/1‑150 (MQ=60)
                AAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCCCCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGCATTATC                                                                                                                                       >  SRR19973070.37153/1‑150 (MQ=60)
                 AGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAATATGCGCCACCATTAAAACTCACATAAGAAATGCCGCCCCCTTATCCGACAAAGGCGAATTATCT                                                                                                                                      >  SRR19973070.1887925/1‑150 (MQ=60)
                  GAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTT                                                                                                                                     <  SRR19973070.641505/150‑1 (MQ=60)
                  GAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTT                                                                                                                                     <  SRR19973070.641540/150‑1 (MQ=60)
                   AGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTA                                                                                                                                    <  SRR19973070.3214861/150‑1 (MQ=60)
                   AGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTA                                                                                                                                    >  SRR19973070.2123815/1‑150 (MQ=60)
                    GACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTAT                                                                                                                                   >  SRR19973070.2450368/1‑150 (MQ=60)
                      CTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAATATGAGAGAAGATGCGCCACCCATAAACCTCACAGAAGAAATGCCGCCCCCATTTCCGACAAAGGCGAATTATATTATTC                                                                                                                                 >  SRR19973070.2250824/1‑150 (MQ=60)
                       TGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCA                                                                                                                                <  SRR19973070.2906521/150‑1 (MQ=60)
                        GAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCAT                                                                                                                               <  SRR19973070.496519/150‑1 (MQ=60)
                        GAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCAT                                                                                                                               <  SRR19973070.2170282/150‑1 (MQ=60)
                                ATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCG                                                                                                                       >  SRR19973070.2434159/1‑150 (MQ=60)
                                    GCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAG                                                                                                                   <  SRR19973070.2499881/150‑1 (MQ=60)
                                     CGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGC                                                                                                                  <  SRR19973070.1186487/150‑1 (MQ=60)
                                     CGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGC                                                                                                                  <  SRR19973070.1186380/150‑1 (MQ=60)
                                        TGACGACCACACACTCTACAAACCTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCACCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTC                                                                                                               <  SRR19973070.3076977/150‑1 (MQ=60)
                                        TGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGACGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTC                                                                                                               <  SRR19973070.2697626/150‑1 (MQ=60)
                                        TGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTC                                                                                                               <  SRR19973070.2241893/150‑1 (MQ=60)
                                         GACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCT                                                                                                              <  SRR19973070.3262509/150‑1 (MQ=60)
                                         GACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCT                                                                                                              <  SRR19973070.3262669/150‑1 (MQ=60)
                                          ACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTT                                                                                                             <  SRR19973070.3315627/150‑1 (MQ=60)
                                           CGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTT                                                                                                            <  SRR19973070.3076495/150‑1 (MQ=60)
                                               CACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACA                                                                                                        >  SRR19973070.2449213/1‑150 (MQ=60)
                                                ACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACAT                                                                                                       <  SRR19973070.843629/150‑1 (MQ=60)
                                                ACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACAT                                                                                                       <  SRR19973070.2511015/150‑1 (MQ=60)
                                                ACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACAT                                                                                                       <  SRR19973070.1475738/150‑1 (MQ=60)
                                                   CACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGC                                                                                                    >  SRR19973070.1086539/1‑150 (MQ=60)
                                                      TCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGG                                                                                                 <  SRR19973070.2931306/150‑1 (MQ=60)
                                                      TCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGG                                                                                                 <  SRR19973070.2931271/150‑1 (MQ=60)
                                                      TCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGG                                                                                                 <  SRR19973070.2931287/150‑1 (MQ=60)
                                                       CTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGT                                                                                                <  SRR19973070.902499/150‑1 (MQ=60)
                                                        TACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTG                                                                                               <  SRR19973070.2106118/150‑1 (MQ=60)
                                                              ACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAAT                                                                                         <  SRR19973070.2269367/150‑1 (MQ=60)
                                                              ACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAAT                                                                                         <  SRR19973070.2269333/150‑1 (MQ=60)
                                                                  CAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATG                                                                                     <  SRR19973070.2460835/150‑1 (MQ=60)
                                                                  CAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATG                                                                                     <  SRR19973070.42677/150‑1 (MQ=60)
                                                                   AGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGT                                                                                    <  SRR19973070.2972349/150‑1 (MQ=60)
                                                                     CCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCT                                                                                  <  SRR19973070.2269881/150‑1 (MQ=60)
                                                                     CCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCT                                                                                  <  SRR19973070.1438210/150‑1 (MQ=60)
                                                                      CATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTG                                                                                 <  SRR19973070.1242760/150‑1 (MQ=60)
                                                                        TCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGAT                                                                               <  SRR19973070.2463895/150‑1 (MQ=60)
                                                                                GATTTTTTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGT                                                                       <  SRR19973070.1047640/150‑1 (MQ=60)
                                                                                GATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGT                                                                       <  SRR19973070.2712058/150‑1 (MQ=60)
                                                                                GATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGT                                                                       >  SRR19973070.1448462/1‑150 (MQ=60)
                                                                                GATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGT                                                                       >  SRR19973070.1448461/1‑150 (MQ=60)
                                                                                  TTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGA                                                                     <  SRR19973070.1427400/150‑1 (MQ=60)
                                                                                     TGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACA                                                                  <  SRR19973070.108679/150‑1 (MQ=60)
                                                                                      GTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACAC                                                                 >  SRR19973070.3029691/1‑150 (MQ=60)
                                                                                      GTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACAC                                                                 >  SRR19973070.3029669/1‑150 (MQ=60)
                                                                                      GTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACAC                                                                 >  SRR19973070.882760/1‑150 (MQ=60)
                                                                                      GTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACAC                                                                 >  SRR19973070.1371533/1‑150 (MQ=60)
                                                                                       TTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACC                                                                >  SRR19973070.2737914/1‑150 (MQ=60)
                                                                                       TTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACC                                                                >  SRR19973070.3332396/1‑150 (MQ=60)
                                                                                        TTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCT                                                               <  SRR19973070.1935926/150‑1 (MQ=60)
                                                                                        TTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCT                                                               <  SRR19973070.2777141/150‑1 (MQ=60)
                                                                                           AGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCC                                                            <  SRR19973070.2558357/150‑1 (MQ=60)
                                                                                                AGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAAT                                                       <  SRR19973070.152348/150‑1 (MQ=60)
                                                                                                 GAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATG                                                      <  SRR19973070.757887/150‑1 (MQ=60)
                                                                                                   GAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAG                                                    <  SRR19973070.292878/150‑1 (MQ=60)
                                                                                                   GAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAG                                                    >  SRR19973070.1202803/1‑150 (MQ=60)
                                                                                                   GAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAG                                                    <  SRR19973070.2621085/150‑1 (MQ=60)
                                                                                                   GAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAG                                                    <  SRR19973070.2620509/150‑1 (MQ=60)
                                                                                                      GATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCA                                                 <  SRR19973070.210159/150‑1 (MQ=60)
                                                                                                         GCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCC                                              <  SRR19973070.2559894/150‑1 (MQ=60)
                                                                                                          CGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCT                                             <  SRR19973070.1975731/150‑1 (MQ=60)
                                                                                                          CGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCT                                             <  SRR19973070.291708/150‑1 (MQ=60)
                                                                                                              ACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACC                                         >  SRR19973070.2585033/1‑150 (MQ=60)
                                                                                                               CCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCC                                        >  SRR19973070.517402/1‑150 (MQ=60)
                                                                                                               CCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCC                                        >  SRR19973070.2231849/1‑150 (MQ=60)
                                                                                                               CCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCC                                        >  SRR19973070.2962767/1‑150 (MQ=60)
                                                                                                                CATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCA                                       >  SRR19973070.2483193/1‑150 (MQ=60)
                                                                                                                CATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCA                                       >  SRR19973070.1009461/1‑150 (MQ=60)
                                                                                                                    AAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTAC                                   >  SRR19973070.2420118/1‑150 (MQ=60)
                                                                                                                     AACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACC                                  >  SRR19973070.2376141/1‑150 (MQ=60)
                                                                                                                     AACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACC                                  <  SRR19973070.1599262/150‑1 (MQ=60)
                                                                                                                     AACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACC                                  <  SRR19973070.2532926/150‑1 (MQ=60)
                                                                                                                          CACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTG                             >  SRR19973070.1997073/1‑150 (MQ=60)
                                                                                                                             AGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCA                          >  SRR19973070.331466/1‑150 (MQ=60)
                                                                                                                             AGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCA                          >  SRR19973070.331444/1‑150 (MQ=60)
                                                                                                                             AGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCA                          >  SRR19973070.1211380/1‑150 (MQ=60)
                                                                                                                             AGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCA                          >  SRR19973070.1211063/1‑150 (MQ=60)
                                                                                                                               AAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATA                        >  SRR19973070.2905009/1‑150 (MQ=60)
                                                                                                                                              CTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCG         >  SRR19973070.1979838/1‑150 (MQ=60)
                                                                                                                                              CTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCG         >  SRR19973070.1979832/1‑150 (MQ=60)
                                                                                                                                              CTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTCACCCACTACCGCCTGCCATACCCCACCATAAAGCG         >  SRR19973070.1845094/1‑150 (MQ=60)
                                                                                                                                                     TACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATC  <  SRR19973070.2820317/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AGTGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATC  >  CP009273/2488267‑2488565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑