Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,488,416 G→T 100% P80P (CCG→CCT)  ypdI → putative lipoprotein involved in colanic acid biosynthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,488,4160GT98.1% 674.0 / 0.9 213P80P (CCG→CCT) ypdIputative lipoprotein involved in colanic acid biosynthesis
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (117/91);  total (119/94)
Fisher's exact test for biased strand distribution p-value = 3.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 213 total aligned reads displayed.

TGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATC  >  CP009273/2488269‑2488562
                                                                                                                                                   |                                                                                                                                                  
tGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTAc                                                                                                                                                  <  2:2739377/150‑1 (MQ=255)
tGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTAc                                                                                                                                                  >  1:2523784/1‑150 (MQ=255)
 gagaGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACa                                                                                                                                                 >  2:2897445/1‑150 (MQ=255)
   gaggagCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACaaa                                                                                                                                               >  2:2739319/1‑150 (MQ=255)
    aggagCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAg                                                                                                                                              >  1:3618285/1‑150 (MQ=255)
      gagCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTATTCCTACAAAGGc                                                                                                                                            >  2:5091207/1‑150 (MQ=255)
        gCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGa                                                                                                                                          <  1:2700088/150‑1 (MQ=255)
        gCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGa                                                                                                                                          <  1:3539944/150‑1 (MQ=255)
         cTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGaa                                                                                                                                         >  2:3567613/1‑150 (MQ=255)
          tataAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAAt                                                                                                                                        <  1:4790355/150‑1 (MQ=255)
           ataAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAAtt                                                                                                                                       <  1:1847231/150‑1 (MQ=255)
           ataAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAAtt                                                                                                                                       <  1:5066168/150‑1 (MQ=255)
             aaaTAGAATGAAAAAACATCAGAGATGACGACCACACACTCTACTAAACTTCATCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATTCGCAAAAATTAAACCTCACATAAGATATGCCGCCCCCTTTTCCTACAAAGGCGAATTAt                                                                                                                                     <  2:3206474/150‑1 (MQ=255)
             aaaGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTAt                                                                                                                                     >  1:1076647/1‑150 (MQ=255)
              aaGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATc                                                                                                                                    <  1:2609811/150‑1 (MQ=255)
                gagaCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCtt                                                                                                                                  >  1:4618644/1‑150 (MQ=255)
                gagaCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCtt                                                                                                                                  <  1:1583674/150‑1 (MQ=255)
                 agaCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTa                                                                                                                                 <  2:636577/150‑1 (MQ=255)
                 agaCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTa                                                                                                                                 <  2:457097/150‑1 (MQ=255)
                 agaCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTa                                                                                                                                 >  2:2050068/1‑150 (MQ=255)
                 agaCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTa                                                                                                                                 >  2:4241240/1‑150 (MQ=255)
                   aCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTAtt                                                                                                                               >  2:4421755/1‑150 (MQ=255)
                      gAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCAt                                                                                                                            >  1:895874/1‑150 (MQ=255)
                       aaaaaaCATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATc                                                                                                                           >  1:2120090/1‑150 (MQ=255)
                        aaaaaCATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATcc                                                                                                                          <  1:2205881/150‑1 (MQ=255)
                              aTCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCg                                                                                                                    <  1:3520602/150‑1 (MQ=255)
                              aTCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCg                                                                                                                    >  1:3830220/1‑150 (MQ=255)
                              aTCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCATTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCg                                                                                                                    >  1:2893615/1‑150 (MQ=255)
                                    gATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCg                                                                                                              <  1:3033074/150‑1 (MQ=255)
                                       gacgacCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCt                                                                                                           >  2:686077/1‑150 (MQ=255)
                                        acgacCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCtt                                                                                                          <  1:4922980/150‑1 (MQ=255)
                                         cgacCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCttt                                                                                                         <  1:188709/150‑1 (MQ=255)
                                         cgacCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCttt                                                                                                         >  1:3638530/1‑150 (MQ=255)
                                         cgacCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCttt                                                                                                         >  1:1711863/1‑150 (MQ=255)
                                            ccACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTcac                                                                                                      >  1:3120196/1‑150 (MQ=255)
                                             cacacaCTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTcaca                                                                                                     >  1:1793149/1‑150 (MQ=255)
                                              acacacTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACAt                                                                                                    <  2:4567312/150‑1 (MQ=255)
                                              acacacTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCGGTGCGATAGCGTCTTTCACAt                                                                                                    >  2:3391054/1‑150 (MQ=255)
                                                acacTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATgg                                                                                                  <  1:4358120/150‑1 (MQ=255)
                                                  acTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCa                                                                                                >  1:1369094/1‑150 (MQ=255)
                                                   ctctACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAg                                                                                               >  1:1155195/1‑150 (MQ=255)
                                                      tACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTg                                                                                            <  2:2978528/150‑1 (MQ=255)
                                                      tACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTg                                                                                            >  1:3133212/1‑150 (MQ=255)
                                                       aCAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGa                                                                                           <  2:3823826/150‑1 (MQ=255)
                                                            aCTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAAt                                                                                      >  1:4747250/1‑150 (MQ=255)
                                                             cTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATc                                                                                     >  2:2815911/1‑150 (MQ=255)
                                                             cTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATc                                                                                     >  2:221762/1‑150 (MQ=255)
                                                              ttCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCa                                                                                    >  2:247487/1‑150 (MQ=255)
                                                               tCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCAACATTAAAACTCACATAAGAAATGCCGCCCCCTTTTACTACAAATGCGAATTATCTTATTAATACTTTGCGATATAGTCTTTCACAAGGAAGGTTAACAATCAt                                                                                   >  1:1548493/1‑150 (MQ=255)
                                                                 aGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGt                                                                                 >  2:967940/1‑150 (MQ=255)
                                                                   ccATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTct                                                                               <  2:5071247/150‑1 (MQ=255)
                                                                      tCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTctgat                                                                            <  1:69025/150‑1 (MQ=255)
                                                                         caGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCCCAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTctgatgct                                                                         <  2:1837253/150‑1 (MQ=255)
                                                                         caGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTctgatgct                                                                         <  2:4158420/150‑1 (MQ=255)
                                                                         caGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTctgatgct                                                                         <  1:2337249/150‑1 (MQ=255)
                                                                          aGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTctgatgctg                                                                        <  1:790692/150‑1 (MQ=255)
                                                                            gCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTctgatgctgat                                                                      <  1:2397116/150‑1 (MQ=255)
                                                                             cGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTctgatgctgatg                                                                     >  2:4632447/1‑150 (MQ=255)
                                                                               aTTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGAtgtg                                                                   >  2:4095322/1‑150 (MQ=255)
                                                                               aTTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGAtgtg                                                                   <  2:2698196/150‑1 (MQ=255)
                                                                                   tGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAaca                                                               <  1:4014124/150‑1 (MQ=255)
                                                                                      ttAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCt                                                            <  2:1043237/150‑1 (MQ=255)
                                                                                       tAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTg                                                           >  1:4707194/1‑150 (MQ=255)
                                                                                       tAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTg                                                           >  1:4458046/1‑150 (MQ=255)
                                                                                        aaGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGc                                                          >  2:230855/1‑150 (MQ=255)
                                                                                          gATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGccc                                                        <  1:923357/150‑1 (MQ=255)
                                                                                              agagaAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAAt                                                    <  1:247487/150‑1 (MQ=255)
                                                                                              agagaAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAAt                                                    >  1:3565810/1‑150 (MQ=255)
                                                                                               gagaAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATg                                                   <  2:1974114/150‑1 (MQ=255)
                                                                                               gagaAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATg                                                   >  1:1671320/1‑150 (MQ=255)
                                                                                                 gaagaTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAg                                                 >  2:769494/1‑150 (MQ=255)
                                                                                                   agaTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGAGTCTTTCAAATGGAAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGcc                                               >  1:2241445/1‑150 (MQ=255)
                                                                                                        cgcCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCt                                          <  1:2739319/150‑1 (MQ=255)
                                                                                                         tccaccaTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTg                                         >  2:4652275/2‑150 (MQ=255)
                                                                                                         gcCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTg                                         <  1:3351358/150‑1 (MQ=255)
                                                                                                          ccaccaTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGa                                        <  1:1240874/150‑1 (MQ=255)
                                                                                                          ccaccaTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGa                                        <  2:4963636/150‑1 (MQ=255)
                                                                                                           caccaTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGAc                                       >  2:2728002/1‑150 (MQ=255)
                                                                                                            accaTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGAcc                                      >  1:661674/1‑150 (MQ=255)
                                                                                                               aTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCAc                                   <  2:3953817/150‑1 (MQ=255)
                                                                                                                ttAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACt                                  >  2:1713909/1‑150 (MQ=255)
                                                                                                                  aaaCCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTAc                                >  2:1306246/1‑150 (MQ=255)
                                                                                                                    aCCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCg                              <  2:3933350/150‑1 (MQ=255)
                                                                                                                    aCCTCACAGAAGAAATGCCGCCCACTTTTCCTTCAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACAGGGCAGGTGAACAATCATTTCTGATGCTGATGTGAACACCTGCCCTAATGAGCCATCCTGACCCACTACCg                              <  2:3680760/150‑1 (MQ=255)
                                                                                                                      cTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGcc                            >  1:4230848/1‑150 (MQ=255)
                                                                                                                      cTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGcc                            >  2:2423848/1‑150 (MQ=255)
                                                                                                                       tCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCt                           <  2:2518101/150‑1 (MQ=255)
                                                                                                                        cacaGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTg                          >  2:4769858/1‑150 (MQ=255)
                                                                                                                        cacaGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTg                          <  2:1349293/150‑1 (MQ=255)
                                                                                                                         acaGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGc                         <  2:3120196/150‑1 (MQ=255)
                                                                                                                             aagaaATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATa                     >  2:4489673/1‑150 (MQ=255)
                                                                                                                             aagaaATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATa                     <  1:2057298/150‑1 (MQ=255)
                                                                                                                              agaaATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCAGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATAc                    >  2:2950688/1‑150 (MQ=255)
                                                                                                                                 aaTGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATAcccc                 <  1:3983702/150‑1 (MQ=255)
                                                                                                                                 aaTGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATAcccc                 <  1:3798443/150‑1 (MQ=255)
                                                                                                                                   tGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCccac               >  1:3750996/1‑150 (MQ=255)
                                                                                                                                       gccCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATa           <  1:599772/150‑1 (MQ=255)
                                                                                                                                         ccccTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCAGaaa         >  1:391024/1‑150 (MQ=255)
                                                                                                                                          cccTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAg        <  2:3037904/150‑1 (MQ=255)
                                                                                                                                             ttttCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGa     >  2:4361797/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATC  >  CP009273/2488269‑2488562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCG  >  CP009273/2488267‑2488558
                                                                                                                                                     |                                                                                                                                              
AGTGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCT                                                                                                                                                <  SRR19973063.3060731/150‑1 (MQ=60)
 GTGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTA                                                                                                                                               <  SRR19973063.316056/150‑1 (MQ=60)
  TGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTAC                                                                                                                                              >  SRR19973063.2531833/1‑150 (MQ=60)
      AGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAG                                                                                                                                          >  SRR19973063.3629896/1‑150 (MQ=60)
      AGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAG                                                                                                                                          >  SRR19973063.3629897/1‑150 (MQ=60)
          GCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGA                                                                                                                                      <  SRR19973063.3551286/150‑1 (MQ=60)
          GCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGA                                                                                                                                      <  SRR19973063.2713772/150‑1 (MQ=60)
          GCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGA                                                                                                                                      <  SRR19973063.2708794/150‑1 (MQ=60)
            TATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAAT                                                                                                                                    <  SRR19973063.4805660/150‑1 (MQ=60)
             ATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATT                                                                                                                                   <  SRR19973063.5082471/150‑1 (MQ=60)
             ATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATT                                                                                                                                   <  SRR19973063.1852995/150‑1 (MQ=60)
               AAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCCTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTAT                                                                                                                                 >  SRR19973063.1079914/1‑150 (MQ=60)
                AAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATC                                                                                                                                <  SRR19973063.2618108/150‑1 (MQ=60)
                  GAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTT                                                                                                                              >  SRR19973063.4633402/1‑150 (MQ=60)
                  GAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTT                                                                                                                              <  SRR19973063.1588526/150‑1 (MQ=60)
                   AGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTA                                                                                                                             <  SRR19973063.4987391/150‑1 (MQ=60)
                   AGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTA                                                                                                                             >  SRR19973063.62533/1‑150 (MQ=60)
                   AGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTA                                                                                                                             >  SRR19973063.187109/1‑150 (MQ=60)
                   AGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTA                                                                                                                             <  SRR19973063.1754928/150‑1 (MQ=60)
                        GAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCAT                                                                                                                        >  SRR19973063.898483/1‑150 (MQ=60)
                         AAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATC                                                                                                                       >  SRR19973063.2126719/1‑150 (MQ=60)
                          AAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCC                                                                                                                      <  SRR19973063.2212819/150‑1 (MQ=60)
                          AAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCC                                                                                                                      <  SRR19973063.2212828/150‑1 (MQ=60)
                             AACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGT                                                                                                                   <  SRR19973063.1295478/150‑1 (MQ=60)
                               CATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGC                                                                                                                 >  SRR19973063.809916/1‑150 (MQ=60)
                                ATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCG                                                                                                                <  SRR19973063.3532174/150‑1 (MQ=60)
                                ATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCG                                                                                                                >  SRR19973063.3842347/1‑150 (MQ=60)
                                ATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCG                                                                                                                >  SRR19973063.3842405/1‑150 (MQ=60)
                                ATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCG                                                                                                                <  SRR19973063.3531869/150‑1 (MQ=60)
                                ATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATACTGTGCG                                                                                                                >  SRR19973063.5102474/1‑150 (MQ=60)
                                ATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCATTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCG                                                                                                                >  SRR19973063.2902848/1‑150 (MQ=60)
                                  CAGCGACGACGACCACCCACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGAT                                                                                                              <  SRR19973063.2630286/150‑1 (MQ=60)
                                      GATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCG                                                                                                          <  SRR19973063.3042762/150‑1 (MQ=60)
                                      GATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCG                                                                                                          <  SRR19973063.1998652/150‑1 (MQ=60)
                                       ATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGT                                                                                                         >  SRR19973063.680123/1‑150 (MQ=60)
                                       ATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGT                                                                                                         >  SRR19973063.3134309/1‑150 (MQ=60)
                                        TGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTC                                                                                                        <  SRR19973063.3911172/150‑1 (MQ=60)
                                        TGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTC                                                                                                        >  SRR19973063.2955172/1‑150 (MQ=60)
                                          ACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTT                                                                                                      <  SRR19973063.4938876/150‑1 (MQ=60)
                                           CGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAATATGCGCCACCATTAAACCTCAAAGAAGAAATTCCGCCCCCTTTTCCAACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTT                                                                                                     >  SRR19973063.3650344/1‑150 (MQ=60)
                                           CGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCCCCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTT                                                                                                     >  SRR19973063.913299/1‑150 (MQ=60)
                                           CGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTT                                                                                                     >  SRR19973063.1717082/1‑150 (MQ=60)
                                           CGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTT                                                                                                     >  SRR19973063.3650204/1‑150 (MQ=60)
                                           CGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTT                                                                                                     <  SRR19973063.189290/150‑1 (MQ=60)
                                            GACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTC                                                                                                    <  SRR19973063.4435951/150‑1 (MQ=60)
                                              CCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCAC                                                                                                  >  SRR19973063.3130242/1‑150 (MQ=60)
                                               CACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACA                                                                                                 >  SRR19973063.1798751/1‑150 (MQ=60)
                                               CACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACA                                                                                                 >  SRR19973063.1798696/1‑150 (MQ=60)
                                                ACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACAT                                                                                                <  SRR19973063.4004775/150‑1 (MQ=60)
                                                 CACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATG                                                                                               >  SRR19973063.1692792/1‑150 (MQ=60)
                                                  ACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGG                                                                                              <  SRR19973063.4372050/150‑1 (MQ=60)
                                                    ACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCA                                                                                            >  SRR19973063.1373219/1‑150 (MQ=60)
                                                     CTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAG                                                                                           >  SRR19973063.1158680/1‑150 (MQ=60)
                                                       CTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGT                                                                                         <  SRR19973063.3555241/150‑1 (MQ=60)
                                                        TACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTG                                                                                        >  SRR19973063.3143293/1‑150 (MQ=60)
                                                        TACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTG                                                                                        >  SRR19973063.540797/1‑150 (MQ=60)
                                                            AAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACA                                                                                    <  SRR19973063.416979/150‑1 (MQ=60)
                                                             AACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTACTACAAAGGCGAATTATCTTATTAATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAA                                                                                   >  SRR19973063.1664901/1‑150 (MQ=60)
                                                              ACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAAT                                                                                  >  SRR19973063.4762366/1‑150 (MQ=60)
                                                               CTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATC                                                                                 >  SRR19973063.2430688/1‑150 (MQ=60)
                                                                 TCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCAT                                                                               >  SRR19973063.3297085/1‑150 (MQ=60)
                                                                 TCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCAT                                                                               >  SRR19973063.1553476/1‑150 (MQ=60)
                                                                 TCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCAACATTAAAACTCACATAAGAAATGCCGCCCCCTTTTACTACAAATGCGAATTATCTTATTAATACTTTGCGATATAGTCTTTCACAAGGAAGGTTAACAATCAT                                                                               >  SRR19973063.1553237/1‑150 (MQ=60)
                                                                  CAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATG                                                                              >  SRR19973063.4891006/1‑150 (MQ=60)
                                                                        TCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGAT                                                                        <  SRR19973063.69266/150‑1 (MQ=60)
                                                                           CAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCT                                                                     <  SRR19973063.2344637/150‑1 (MQ=60)
                                                                           CAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCT                                                                     <  SRR19973063.2344721/150‑1 (MQ=60)
                                                                            AGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTG                                                                    <  SRR19973063.792978/150‑1 (MQ=60)
                                                                            AGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTG                                                                    >  SRR19973063.211611/1‑150 (MQ=60)
                                                                              GCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGAT                                                                  <  SRR19973063.4345193/150‑1 (MQ=60)
                                                                              GCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGAT                                                                  <  SRR19973063.2404786/150‑1 (MQ=60)
                                                                              GCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGAT                                                                  <  SRR19973063.2404741/150‑1 (MQ=60)
                                                                               CGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATG                                                                 >  SRR19973063.120039/1‑150 (MQ=60)
                                                                                     TGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACA                                                           <  SRR19973063.4027004/150‑1 (MQ=60)
                                                                                         TAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTG                                                       <  SRR19973063.76734/150‑1 (MQ=60)
                                                                                         TAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTG                                                       >  SRR19973063.4722191/1‑150 (MQ=60)
                                                                                         TAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTG                                                       >  SRR19973063.4472371/1‑150 (MQ=60)
                                                                                         TAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTG                                                       >  SRR19973063.4472354/1‑150 (MQ=60)
                                                                                          AAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGC                                                      >  SRR19973063.981682/1‑150 (MQ=60)
                                                                                            GATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCC                                                    >  SRR19973063.2394526/1‑150 (MQ=60)
                                                                                            GATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCC                                                    >  SRR19973063.2512745/1‑150 (MQ=60)
                                                                                            GATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCC                                                    <  SRR19973063.926095/150‑1 (MQ=60)
                                                                                                AGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAAT                                                >  SRR19973063.3577468/1‑150 (MQ=60)
                                                                                                AGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAAT                                                <  SRR19973063.248239/150‑1 (MQ=60)
                                                                                                AGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAAT                                                >  SRR19973063.3577242/1‑150 (MQ=60)
                                                                                                 GAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATG                                               >  SRR19973063.1676415/1‑150 (MQ=60)
                                                                                                     AGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCC                                           >  SRR19973063.3056722/1‑150 (MQ=60)
                                                                                                     AGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCC                                           >  SRR19973063.3056825/1‑150 (MQ=60)
                                                                                                     AGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCC                                           <  SRR19973063.4746154/150‑1 (MQ=60)
                                                                                                     AGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGAGTCTTTCAAATGGAAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCC                                           >  SRR19973063.2248567/1‑150 (MQ=60)
                                                                                                          CGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCT                                      <  SRR19973063.2748165/150‑1 (MQ=60)
                                                                                                          CGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCT                                      <  SRR19973063.2748205/150‑1 (MQ=60)
                                                                                                          CGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCT                                      >  SRR19973063.2223695/1‑150 (MQ=60)
                                                                                                           GCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTG                                     <  SRR19973063.3362030/150‑1 (MQ=60)
                                                                                                            CCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGA                                    <  SRR19973063.1244578/150‑1 (MQ=60)
                                                                                                              ACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACC                                  <  SRR19973063.1727824/150‑1 (MQ=60)
                                                                                                              ACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACC                                  >  SRR19973063.663610/1‑150 (MQ=60)
                                                                                                                     AACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACC                           <  SRR19973063.1113818/150‑1 (MQ=60)
                                                                                                                       CCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGC                         <  SRR19973063.4270169/150‑1 (MQ=60)
                                                                                                                        CTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCC                        >  SRR19973063.4244333/1‑150 (MQ=60)
                                                                                                                          CACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTG                      <  SRR19973063.364764/150‑1 (MQ=60)
                                                                                                                          CACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTG                      >  SRR19973063.3399243/1‑150 (MQ=60)
                                                                                                                               AAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATA                 <  SRR19973063.2063726/150‑1 (MQ=60)
                                                                                                                                  AAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCC              <  SRR19973063.2600915/150‑1 (MQ=60)
                                                                                                                                   AATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCC             <  SRR19973063.3996469/150‑1 (MQ=60)
                                                                                                                                   AATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCC             <  SRR19973063.3810546/150‑1 (MQ=60)
                                                                                                                                   AATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCC             <  SRR19973063.3807166/150‑1 (MQ=60)
                                                                                                                                     TGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCAC           >  SRR19973063.3762980/1‑150 (MQ=60)
                                                                                                                                      GCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACC          >  SRR19973063.464863/1‑150 (MQ=60)
                                                                                                                                       CCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCA         <  SRR19973063.397733/150‑1 (MQ=60)
                                                                                                                                         GCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATA       <  SRR19973063.601536/150‑1 (MQ=60)
                                                                                                                                          CCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAA      >  SRR19973063.1379928/1‑150 (MQ=60)
                                                                                                                                           CCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCAGAAA     >  SRR19973063.392134/1‑150 (MQ=60)
                                                                                                                                              CTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCG  >  SRR19973063.4804771/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                              
AGTGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCG  >  CP009273/2488267‑2488558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑