Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,488,416 G→T 100% P80P (CCG→CCT)  ypdI → putative lipoprotein involved in colanic acid biosynthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,488,4160GT100.0% 817.7 / ‑6.7 268P80P (CCG→CCT) ypdIputative lipoprotein involved in colanic acid biosynthesis
Reads supporting (aligned to +/- strand):  ref base G (0/4);  major base T (106/153);  minor base A (5/0);  total (111/157)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 268 total aligned reads displayed.

TGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATC  >  CP009273/2488269‑2488562
                                                                                                                                                   |                                                                                                                                                  
tGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTAc                                                                                                                                                  <  2:3342443/150‑1 (MQ=255)
tGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTAc                                                                                                                                                  <  1:2797628/150‑1 (MQ=255)
 gagaGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACa                                                                                                                                                 <  2:4171094/150‑1 (MQ=255)
  agagGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCCTTTCCTACaa                                                                                                                                                >  1:245580/1‑150 (MQ=255)
   gaggagCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACaaa                                                                                                                                               <  2:1938446/150‑1 (MQ=255)
    aggttcTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCTCAGGCGATTTTGTTTAAGATGAGAGAAGTTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAg                                                                                                                                              <  1:1643721/145‑1 (MQ=255)
      gagCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGc                                                                                                                                            <  1:1407581/150‑1 (MQ=255)
      gagCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCACCCCCTTTTCCGACAAAGGc                                                                                                                                            <  1:5302423/150‑1 (MQ=255)
       agCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCg                                                                                                                                           >  2:1703203/1‑150 (MQ=255)
          tataAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAAt                                                                                                                                        >  2:4614880/1‑150 (MQ=255)
          tataAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAAt                                                                                                                                        >  2:5103019/1‑150 (MQ=255)
           ataAAGAGACTTAAAAAACATCAGCGATGACTACCACACACTCTACAAAACTTCAGCCATCAGAGGCGATTTTTTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTAACAGAAGAAATGCCTCCCCCTTTTCCTACAAAGGCGAAtt                                                                                                                                       <  2:4745372/150‑1 (MQ=255)
           ataAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAAtt                                                                                                                                       >  1:399068/1‑150 (MQ=255)
            taAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCAGCCCCCTTTTCCTACAAAGGCGAATTa                                                                                                                                      <  2:5172375/150‑1 (MQ=255)
              aaGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATc                                                                                                                                    <  2:1530828/150‑1 (MQ=255)
               agagACTGAAAAAACATCAGCGATGACGACCCCACACTCTACAACCCTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATGAAACCTCCCAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCt                                                                                                                                   <  2:1190533/150‑1 (MQ=255)
               agagACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCt                                                                                                                                   <  2:4806518/150‑1 (MQ=255)
                 agaCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTa                                                                                                                                 <  2:3996701/150‑1 (MQ=255)
                 agaCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCATTTCCTACAAAGGCGACTTAGCTTa                                                                                                                                 >  2:4418913/1‑150 (MQ=255)
                  gaCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTAt                                                                                                                                <  1:1557740/150‑1 (MQ=255)
                  gaCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTAt                                                                                                                                <  1:4782163/150‑1 (MQ=255)
                  gaCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTAt                                                                                                                                <  1:4305737/150‑1 (MQ=255)
                  gaCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTAt                                                                                                                                <  2:1487555/150‑1 (MQ=255)
                   aCTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTAtt                                                                                                                               <  1:183297/150‑1 (MQ=255)
                    cTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTc                                                                                                                              <  1:3523185/150‑1 (MQ=255)
                     tGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCa                                                                                                                             >  2:4339742/1‑150 (MQ=255)
                       aaaaaaCATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATc                                                                                                                           <  1:90919/150‑1 (MQ=255)
                         aaaaCATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAAACTCACAGAAGAAATGACGCCCCCTTTTCCTACAAATGCGAATTATCTTATTCATACt                                                                                                                         >  1:3750106/1‑150 (MQ=255)
                          aaaCATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCAGTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCtg                                                                                                                        >  2:2713263/1‑150 (MQ=255)
                              aTCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCg                                                                                                                    <  1:4715017/150‑1 (MQ=255)
                              aTCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCATATTCATCCTGTGCg                                                                                                                    >  1:7097/1‑150 (MQ=255)
                                 aGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTTTTTAATATGATAGAAGATTCGCCACCATTAAACCACACAGAAGAAATTCCGCCCCCTTTTCCTACAAAGGCGAATTATATTATTAATCCAGTGCGATa                                                                                                                 >  1:896665/1‑150 (MQ=255)
                                  gCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAg                                                                                                                <  2:4723820/150‑1 (MQ=255)
                                   cGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGc                                                                                                               <  1:4382766/150‑1 (MQ=255)
                                    gATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCg                                                                                                              <  1:637782/150‑1 (MQ=255)
                                     aTGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGt                                                                                                             >  1:1986403/1‑150 (MQ=255)
                                        acgacCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCtt                                                                                                          <  1:1208761/150‑1 (MQ=255)
                                          gacCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTc                                                                                                        <  1:4147753/150‑1 (MQ=255)
                                            ccACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCAGTGCGATAGCGTCTTTcac                                                                                                      <  1:370008/150‑1 (MQ=255)
                                              acacacTCTACAAAACTTCAGCCATCACTGGCGATTTTGTTTAAGATGAGAGAAGATGCGCAACCATTAAACCTCACAGAAGAAATGCCGCCCCATTTTCCTACAAAGGCGAATTATCGTATTCATCCTGTGCGATAGCGTCTTTCACAt                                                                                                    <  1:1066816/150‑1 (MQ=255)
                                                acacTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATgg                                                                                                  >  2:2598288/1‑150 (MQ=255)
                                                  acTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCa                                                                                                <  1:5038642/150‑1 (MQ=255)
                                                   ctctACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAg                                                                                               >  2:1659629/1‑150 (MQ=255)
                                                     ctACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGt                                                                                             >  2:4872321/1‑150 (MQ=255)
                                                       aCAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGa                                                                                           <  2:937419/150‑1 (MQ=255)
                                                        cAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGaa                                                                                          <  1:3384846/150‑1 (MQ=255)
                                                        cAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGaa                                                                                          >  2:383533/1‑150 (MQ=255)
                                                         aaaaCTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAAc                                                                                         >  2:2204084/1‑150 (MQ=255)
                                                             cTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCAGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATc                                                                                     <  1:3415926/150‑1 (MQ=255)
                                                               tCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCAt                                                                                   <  1:3578052/150‑1 (MQ=255)
                                                               tCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCAt                                                                                   <  2:2054068/150‑1 (MQ=255)
                                                                cAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATg                                                                                  <  1:1695468/150‑1 (MQ=255)
                                                                 aGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGt                                                                                 <  2:3741526/150‑1 (MQ=255)
                                                                 aGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGt                                                                                 >  2:977286/1‑150 (MQ=255)
                                                                     aTCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTctga                                                                             <  1:86649/150‑1 (MQ=255)
                                                                        acaGGCGATTTTGTTTAAGATGAGATAAGATGCGCCACCATTAAAACTCACAGAAGAAATGCCGCCCCCATTTACAACAAATGCGAATTATATTATTCATCCATTGCGATAGCGTATTTAAAAAGGCAGGTGAACAATAAATTctgatgc                                                                          >  2:4668032/1‑150 (MQ=255)
                                                                          aGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTctgatgctg                                                                        >  2:1457565/1‑150 (MQ=255)
                                                                          aGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTctgatgctg                                                                        >  1:1265639/1‑150 (MQ=255)
                                                                           ggCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTctgatgctga                                                                       >  2:71459/1‑150 (MQ=255)
                                                                                      ttAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCt                                                            <  2:4627301/150‑1 (MQ=255)
                                                                                         aGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGcc                                                         >  1:740966/1‑150 (MQ=255)
                                                                                           aTGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCa                                                       >  1:4839260/1‑150 (MQ=255)
                                                                                           aTGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCa                                                       >  1:2863187/1‑150 (MQ=255)
                                                                                            tGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCaa                                                      >  1:2347041/1‑150 (MQ=255)
                                                                                                agaagaTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCAGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGa                                                  <  2:2805000/150‑1 (MQ=255)
                                                                                                agaagaTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCAGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGa                                                  <  2:1206365/150‑1 (MQ=255)
                                                                                                 gaagaTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAg                                                 >  1:2910856/1‑150 (MQ=255)
                                                                                                  aagaTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGc                                                >  1:4249715/1‑150 (MQ=255)
                                                                                                   agaTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGcc                                               <  1:2995310/150‑1 (MQ=255)
                                                                                                   agaTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGcc                                               <  1:1544976/150‑1 (MQ=255)
                                                                                                   agaTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCAGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGcc                                               >  2:2391738/1‑150 (MQ=255)
                                                                                                    gaTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCa                                              >  2:4148548/1‑150 (MQ=255)
                                                                                                    gaTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCa                                              >  2:2432652/1‑150 (MQ=255)
                                                                                                    gaTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCa                                              <  2:3802581/150‑1 (MQ=255)
                                                                                                     aTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCAt                                             <  2:4202963/150‑1 (MQ=255)
                                                                                                              caTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCa                                    >  2:1101471/1‑150 (MQ=255)
                                                                                                              caTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCa                                    >  1:4179896/1‑150 (MQ=255)
                                                                                                               aTTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCAc                                   <  2:166287/150‑1 (MQ=255)
                                                                                                                ttAAACCTCACAGAAGAAATGCCGCCCCCATTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACt                                  <  1:1391987/150‑1 (MQ=255)
                                                                                                                 tAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTa                                 <  1:3623542/150‑1 (MQ=255)
                                                                                                                  aaaCCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTAc                                >  2:2856882/1‑150 (MQ=255)
                                                                                                                   aaCCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTAcc                               >  2:5321144/1‑150 (MQ=255)
                                                                                                                       tCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCt                           >  2:153922/1‑150 (MQ=255)
                                                                                                                          caGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGcc                        <  1:5103086/150‑1 (MQ=255)
                                                                                                                           agaagaAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGTTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCa                       >  1:5003552/1‑150 (MQ=255)
                                                                                                                           agaagaAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCa                       >  2:3239304/1‑150 (MQ=255)
                                                                                                                           agaagaAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCa                       >  2:3032720/1‑150 (MQ=255)
                                                                                                                              agaaATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATAc                    <  2:5120909/150‑1 (MQ=255)
                                                                                                                               gaaATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATAcc                   <  2:4485644/150‑1 (MQ=255)
                                                                                                                                aaaTGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATAccc                  <  2:179284/150‑1 (MQ=255)
                                                                                                                                 aaTGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATAcccc                 >  2:4942200/1‑150 (MQ=255)
                                                                                                                                 aaTGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATAcccc                 >  2:1832968/1‑150 (MQ=255)
                                                                                                                                 aaTGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATAcccc                 >  2:694191/1‑150 (MQ=255)
                                                                                                                                  aTGCCGCCCCCTTTTCCTACAAAGGCTAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCAGGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCcca                <  1:2713263/150‑1 (MQ=255)
                                                                                                                                       gccCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATa           <  1:2391294/150‑1 (MQ=255)
                                                                                                                                         ccccTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATaaa         >  2:1312639/1‑150 (MQ=255)
                                                                                                                                           ccTTTTCCTACAAAGGAGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGc       <  1:3245230/150‑1 (MQ=255)
                                                                                                                                            cTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCg      >  2:3609200/1‑150 (MQ=255)
                                                                                                                                               ttCCTACAAAGGCGAATTATCTTATTCATCCAGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAat   <  1:1014728/150‑1 (MQ=255)
                                                                                                                                                tCCTACAAAGGCGAATTATCTTATTCATCCAGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAatc  >  1:527563/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATC  >  CP009273/2488269‑2488562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCA  >  CP009273/2488269‑2488563
                                                                                                                                                   |                                                                                                                                                   
TGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTAC                                                                                                                                                   <  SRR19973062.3589796/150‑1 (MQ=60)
TGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTAC                                                                                                                                                   <  SRR19973062.2812425/150‑1 (MQ=60)
  AGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCCTTTCCTACAA                                                                                                                                                 >  SRR19973062.246826/1‑150 (MQ=60)
   GAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTTTTTAAGATGAGATAAGATTCGCCACCATTAAAACTCACAGAATAAAAGCCGCCCCCATTTACTACAAA                                                                                                                                                >  SRR19973062.1409463/1‑150 (MQ=60)
    aggttCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCTCAGGCGATTTTGTTTAAGATGAGAGAAGTTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAG                                                                                                                                               <  SRR19973062.1651816/145‑1 (MQ=60)
    AGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAG                                                                                                                                               <  SRR19973062.4521849/150‑1 (MQ=60)
     GGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGG                                                                                                                                              <  SRR19973062.1567768/150‑1 (MQ=60)
     GGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGG                                                                                                                                              <  SRR19973062.3764442/150‑1 (MQ=60)
      GAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGC                                                                                                                                             <  SRR19973062.1414320/150‑1 (MQ=60)
      GAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCACCCCCTTTTCCGACAAAGGC                                                                                                                                             <  SRR19973062.5330271/150‑1 (MQ=60)
       AGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAATATGAGAGAAGATGCGCCACCAATAAACCACACAGAAGAAATGCCGCCCCCGATACCAACAAAGGCG                                                                                                                                            >  SRR19973062.3614304/1‑150 (MQ=60)
       AGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATTAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCG                                                                                                                                            >  SRR19973062.2929418/1‑150 (MQ=60)
          TATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATTCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCATTTCCTACAAAGGCGAAT                                                                                                                                         >  SRR19973062.1912880/1‑150 (MQ=60)
           ATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATT                                                                                                                                        >  SRR19973062.400833/1‑150 (MQ=60)
           ATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCAATAAACCACACAGAAGAAATGCCGCCCCCATTTCCTACAAAGGCGAATT                                                                                                                                        >  SRR19973062.2868903/1‑150 (MQ=60)
             AAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTAT                                                                                                                                      <  SRR19973062.4244789/150‑1 (MQ=60)
               AGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCT                                                                                                                                    <  SRR19973062.3376828/150‑1 (MQ=60)
                  GACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTAT                                                                                                                                 <  SRR19973062.3165216/150‑1 (MQ=60)
                  GACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTAT                                                                                                                                 <  SRR19973062.1030333/150‑1 (MQ=60)
                  GACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTAT                                                                                                                                 <  SRR19973062.1565423/150‑1 (MQ=60)
                  GACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTAT                                                                                                                                 <  SRR19973062.4327956/150‑1 (MQ=60)
                  GACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTAT                                                                                                                                 <  SRR19973062.4806997/150‑1 (MQ=60)
                  GACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTAT                                                                                                                                 <  SRR19973062.4807131/150‑1 (MQ=60)
                   ACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATT                                                                                                                                <  SRR19973062.184300/150‑1 (MQ=60)
                   ACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATT                                                                                                                                <  SRR19973062.4994571/150‑1 (MQ=60)
                    CTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTC                                                                                                                               <  SRR19973062.3541015/150‑1 (MQ=60)
                    CTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTC                                                                                                                               <  SRR19973062.169008/150‑1 (MQ=60)
                      GAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCAT                                                                                                                             <  SRR19973062.1534282/150‑1 (MQ=60)
                       AAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGTTGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTAATC                                                                                                                            >  SRR19973062.1399518/1‑150 (MQ=60)
                       AAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATC                                                                                                                            <  SRR19973062.91520/150‑1 (MQ=60)
                       AAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTACTACAAAGGCGAAGTATCTTATTCATA                                                                                                                            >  SRR19973062.5045904/1‑150 (MQ=60)
                         AAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAAACTCACAGAAGAAATGACGCCCCCTTTTCCTACAAATGCGAATTATCTTATTCATACT                                                                                                                          >  SRR19973062.3769344/1‑150 (MQ=60)
                          AAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTG                                                                                                                         <  SRR19973062.2382486/150‑1 (MQ=60)
                           AACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGT                                                                                                                        >  SRR19973062.180266/1‑150 (MQ=60)
                             CATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCTAATTATCTTATTCATCCTGTGC                                                                                                                      <  SRR19973062.376145/150‑1 (MQ=60)
                             CATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGC                                                                                                                      <  SRR19973062.3342797/150‑1 (MQ=60)
                              ATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCG                                                                                                                     <  SRR19973062.4739627/150‑1 (MQ=60)
                              ATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCG                                                                                                                     >  SRR19973062.7173/1‑150 (MQ=60)
                              ATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCATATTCATCCTGTGCG                                                                                                                     >  SRR19973062.7152/1‑150 (MQ=60)
                                 AGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTTTTTAATATGATAGAAGATTCGCCACCATTAAACCACACAGAAGAAATTCCGCCCCCTTTTCCTACAAAGGCGAATTATATTATTAATCCAGTGCGATA                                                                                                                  >  SRR19973062.900496/1‑150 (MQ=60)
                                  GAGATGACGAACACACACTCTACAAAACTTATTCCATCACAGGAGATTTTTTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTAACAGAAGAAATGCCGCCCCCTTTTCCTCCAAAGGCGAATTATCTTATTCATCCTGTGCGATAG                                                                                                                 <  SRR19973062.900060/150‑1 (MQ=60)
                                   CGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGC                                                                                                                <  SRR19973062.4405221/150‑1 (MQ=60)
                                   CGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGC                                                                                                                <  SRR19973062.4405231/150‑1 (MQ=60)
                                    GATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCG                                                                                                               <  SRR19973062.640762/150‑1 (MQ=60)
                                    GATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCG                                                                                                               <  SRR19973062.640723/150‑1 (MQ=60)
                                    GATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCG                                                                                                               <  SRR19973062.5002891/150‑1 (MQ=60)
                                     ATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGT                                                                                                              >  SRR19973062.1996631/1‑150 (MQ=60)
                                       GACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACACAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCT                                                                                                            <  SRR19973062.1417720/150‑1 (MQ=60)
                                       GACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCT                                                                                                            <  SRR19973062.90963/150‑1 (MQ=60)
                                        ACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTT                                                                                                           <  SRR19973062.1214394/150‑1 (MQ=60)
                                          GACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTC                                                                                                         <  SRR19973062.4169258/150‑1 (MQ=60)
                                          GACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTC                                                                                                         <  SRR19973062.664570/150‑1 (MQ=60)
                                          GACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCAATAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTC                                                                                                         >  SRR19973062.4188184/1‑150 (MQ=60)
                                            CCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCAGTGCGATAGCGTCTTTCAC                                                                                                       <  SRR19973062.371669/150‑1 (MQ=60)
                                             CACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCAATAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCAACAAAGGCGAAGTATCTTAATCATCCTGTGCGATAGCGTCTTTAACA                                                                                                      >  SRR19973062.4814526/1‑150 (MQ=60)
                                              ACACACTCTACAAAACTTCAGCCATCACTGGCGATTTTGTTTAAGATGAGAGAAGATGCGCAACCATTAAACCTCACAGAAGAAATGCCGCCCCATTTTCCTACAAAGGCGAATTATCGTATTCATCCTGTGCGATAGCGTCTTTCACAT                                                                                                     <  SRR19973062.1071837/150‑1 (MQ=60)
                                              ACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACAT                                                                                                     <  SRR19973062.4169877/150‑1 (MQ=60)
                                              ACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACAT                                                                                                     <  SRR19973062.2178287/150‑1 (MQ=60)
                                               CACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATG                                                                                                    <  SRR19973062.155494/150‑1 (MQ=60)
                                               CACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCAGTGCGATAGCGTCTTTCACATG                                                                                                    >  SRR19973062.4792428/1‑150 (MQ=60)
                                               CACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCAGTGCGATAGCGTCTTTCACATG                                                                                                    >  SRR19973062.1657125/1‑150 (MQ=60)
                                                  ACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCA                                                                                                 <  SRR19973062.5065346/150‑1 (MQ=60)
                                                      TACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTG                                                                                             <  SRR19973062.5200470/150‑1 (MQ=60)
                                                      TACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGACAGCGTCTTTCACATGGCAGGTG                                                                                             <  SRR19973062.868251/150‑1 (MQ=60)
                                                        CAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAA                                                                                           <  SRR19973062.3402238/150‑1 (MQ=60)
                                                         AAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAAC                                                                                          <  SRR19973062.4367264/150‑1 (MQ=60)
                                                          AAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACA                                                                                         <  SRR19973062.1150487/150‑1 (MQ=60)
                                                             CTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATTCCGCCCCCATTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATTGCAGGTGAAAAATC                                                                                      >  SRR19973062.325039/1‑150 (MQ=60)
                                                             CTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCAGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATC                                                                                      <  SRR19973062.3433410/150‑1 (MQ=60)
                                                               TCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCAT                                                                                    <  SRR19973062.3596125/150‑1 (MQ=60)
                                                                CAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATG                                                                                   <  SRR19973062.1703781/150‑1 (MQ=60)
                                                                  GCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTC                                                                                 <  SRR19973062.1359388/150‑1 (MQ=60)
                                                                     ATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGA                                                                              <  SRR19973062.87227/150‑1 (MQ=60)
                                                                      TCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGAT                                                                             <  SRR19973062.2067906/150‑1 (MQ=60)
                                                                      TCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGAT                                                                             <  SRR19973062.4267569/150‑1 (MQ=60)
                                                                       CACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATG                                                                            <  SRR19973062.5070631/150‑1 (MQ=60)
                                                                          AGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTG                                                                         >  SRR19973062.2768261/1‑150 (MQ=60)
                                                                          AGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTG                                                                         >  SRR19973062.242183/1‑150 (MQ=60)
                                                                          AGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTG                                                                         >  SRR19973062.242225/1‑150 (MQ=60)
                                                                          AGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTG                                                                         >  SRR19973062.1271473/1‑150 (MQ=60)
                                                                          AGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTG                                                                         >  SRR19973062.1271469/1‑150 (MQ=60)
                                                                             CGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCAGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATG                                                                      <  SRR19973062.46698/150‑1 (MQ=60)
                                                                                  TTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAAC                                                                 <  SRR19973062.3431674/150‑1 (MQ=60)
                                                                                       TAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTG                                                            <  SRR19973062.3800155/150‑1 (MQ=60)
                                                                                         AGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCC                                                          >  SRR19973062.744328/1‑150 (MQ=60)
                                                                                          GATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCC                                                         <  SRR19973062.5251385/150‑1 (MQ=60)
                                                                                          GATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCAGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCC                                                         <  SRR19973062.3889416/150‑1 (MQ=60)
                                                                                           ATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCA                                                        >  SRR19973062.2878325/1‑150 (MQ=60)
                                                                                           ATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCA                                                        >  SRR19973062.4864267/1‑150 (MQ=60)
                                                                                            TGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAA                                                       >  SRR19973062.2359527/1‑150 (MQ=60)
                                                                                               GAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATG                                                    <  SRR19973062.2888675/150‑1 (MQ=60)
                                                                                                AGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGA                                                   <  SRR19973062.258229/150‑1 (MQ=60)
                                                                                                 GAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAG                                                  >  SRR19973062.2926206/1‑150 (MQ=60)
                                                                                                  AAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGC                                                 >  SRR19973062.4271723/1‑150 (MQ=60)
                                                                                                  AAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGC                                                 >  SRR19973062.3480291/1‑150 (MQ=60)
                                                                                                  AAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCAGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGC                                                 >  SRR19973062.5324269/1‑150 (MQ=60)
                                                                                                   AGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCC                                                <  SRR19973062.3010991/150‑1 (MQ=60)
                                                                                                   AGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCC                                                <  SRR19973062.1552584/150‑1 (MQ=60)
                                                                                                   AGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCC                                                <  SRR19973062.3585242/150‑1 (MQ=60)
                                                                                                        CGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATTGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCT                                           <  SRR19973062.2918315/150‑1 (MQ=60)
                                                                                                          CCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGA                                         >  SRR19973062.3620707/1‑150 (MQ=60)
                                                                                                           CACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGAC                                        <  SRR19973062.778983/150‑1 (MQ=60)
                                                                                                           CACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGAC                                        <  SRR19973062.709931/150‑1 (MQ=60)
                                                                                                            ACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACC                                       >  SRR19973062.5089768/1‑150 (MQ=60)
                                                                                                              CATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCA                                     >  SRR19973062.3689468/1‑150 (MQ=60)
                                                                                                              CATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCA                                     >  SRR19973062.4201585/1‑150 (MQ=60)
                                                                                                                TTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACT                                   <  SRR19973062.1650143/150‑1 (MQ=60)
                                                                                                                TTAAACCTCACAGAAGAAATGCCGCCCCCATTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACT                                   <  SRR19973062.1398574/150‑1 (MQ=60)
                                                                                                                 TAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTA                                  <  SRR19973062.1268360/150‑1 (MQ=60)
                                                                                                                 TAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTA                                  <  SRR19973062.3642055/150‑1 (MQ=60)
                                                                                                                  AAACCTCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTAC                                 >  SRR19973062.617776/1‑150 (MQ=60)
                                                                                                                       TCACAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCT                            <  SRR19973062.2823465/150‑1 (MQ=60)
                                                                                                                          CAGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCC                         <  SRR19973062.5130104/150‑1 (MQ=60)
                                                                                                                           AGAAGAAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGTTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCA                        >  SRR19973062.5030016/1‑150 (MQ=60)
                                                                                                                                AAATGCCGCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCC                   >  SRR19973062.3454933/1‑150 (MQ=60)
                                                                                                                                  ATGCCGCCCCCTTTTCCTACAAAGGCTAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCAGGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCA                 <  SRR19973062.2727536/150‑1 (MQ=60)
                                                                                                                                       GCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATA            >  SRR19973062.4278277/1‑150 (MQ=60)
                                                                                                                                       GCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATA            <  SRR19973062.2404122/150‑1 (MQ=60)
                                                                                                                                       GCCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATA            >  SRR19973062.900406/1‑150 (MQ=60)
                                                                                                                                        CCCCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAA           <  SRR19973062.189800/150‑1 (MQ=60)
                                                                                                                                          CCCTTTTCCTACAAAGGCGAATTATCTTATTCATCCAGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAG         >  SRR19973062.1130731/1‑150 (MQ=60)
                                                                                                                                           CCTTTTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGC        <  SRR19973062.405185/150‑1 (MQ=60)
                                                                                                                                           CCTTTTCCTACAAAGGAGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGC        <  SRR19973062.3262069/150‑1 (MQ=60)
                                                                                                                                               TTCCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAAT    <  SRR19973062.2625501/150‑1 (MQ=60)
                                                                                                                                               TTCCTACAAAGGCGAATTATCTTATTCATCCAGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAAT    <  SRR19973062.1019470/150‑1 (MQ=60)
                                                                                                                                                TCCTACAAAGGCGAATTATCTTATTCATCCAGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATC   >  SRR19973062.530056/1‑150 (MQ=60)
                                                                                                                                                 CCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCA  >  SRR19973062.5075890/1‑150 (MQ=60)
                                                                                                                                                 CCTACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCA  >  SRR19973062.4392480/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                   
TGAGAGGAGCTATAAAGAGACTGAAAAAACATCAGCGATGACGACCACACACTCTACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCA  >  CP009273/2488269‑2488563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑