Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,828,952 C→A 100% T611T (ACG→ACT)  hypF ← carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,828,9520CA100.0% 550.3 / ‑6.7 189T611T (ACG→ACT) hypFcarbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (81/104);  total (81/108)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 189 total aligned reads displayed.

GTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAA  >  CP009273/2828807‑2829096
                                                                                                                                                 |                                                                                                                                                
gTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGt                                                                                                                                                <  1:3260725/148‑1 (MQ=255)
 tgcctgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtg                                                                                                                                               >  2:2070927/1‑148 (MQ=255)
      gccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggctgg                                                                                                                                          >  1:2540363/1‑148 (MQ=255)
      gccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggctgg                                                                                                                                          >  1:345790/1‑148 (MQ=255)
        cAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACAGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGgcg                                                                                                                                        >  1:1877213/1‑148 (MQ=255)
           ttCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGcac                                                                                                                                     >  2:1756176/1‑148 (MQ=255)
            tCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGcaca                                                                                                                                    >  1:2101701/1‑148 (MQ=255)
                ccACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGccc                                                                                                                                >  2:2280345/1‑148 (MQ=255)
                 cACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCa                                                                                                                               <  2:3141209/148‑1 (MQ=255)
                 cACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCa                                                                                                                               <  2:775102/148‑1 (MQ=255)
                  aCTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAg                                                                                                                              >  2:474540/1‑148 (MQ=255)
                       tgcCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTgccg                                                                                                                         <  1:3224687/148‑1 (MQ=255)
                       tgcCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGACTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTgccg                                                                                                                         <  1:1464548/148‑1 (MQ=255)
                       tgcCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGACTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTgccg                                                                                                                         <  1:1464431/148‑1 (MQ=255)
                            gAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTgccgccgcc                                                                                                                    >  1:1640219/1‑148 (MQ=255)
                              aaGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCAc                                                                                                                  >  1:1934467/1‑148 (MQ=255)
                               aGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACt                                                                                                                 >  1:54643/1‑148 (MQ=255)
                                gTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTg                                                                                                                <  2:3040987/148‑1 (MQ=255)
                                 tGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGc                                                                                                               >  1:1274311/1‑148 (MQ=255)
                                 tGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGc                                                                                                               >  1:2924154/1‑148 (MQ=255)
                                 tGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGc                                                                                                               <  2:14126/148‑1 (MQ=255)
                                 tGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGc                                                                                                               <  1:1532235/148‑1 (MQ=255)
                                 tGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGc                                                                                                               <  1:1622209/148‑1 (MQ=255)
                                 gggCGAGATCCAGGTGATGGTCCACCCGCGGCATTGTCACCGGTTGCGTCACGCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCGGGCGCACAGCCCATAGCCGCCGCCACTGc                                                                                                               <  1:1237587/147‑1 (MQ=255)
                                      agaTCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGa                                                                                                          <  1:2726732/148‑1 (MQ=255)
                                      agaTCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGa                                                                                                          >  1:1363964/1‑148 (MQ=255)
                                      agaTCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGa                                                                                                          <  2:2015238/148‑1 (MQ=255)
                                      agaTCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCGTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGa                                                                                                          >  1:1360595/1‑148 (MQ=255)
                                              ttGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGc                                                                                                  >  1:784545/1‑148 (MQ=255)
                                              ttGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGc                                                                                                  <  2:2335325/148‑1 (MQ=255)
                                                 aTTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCca                                                                                               >  1:1739093/1‑148 (MQ=255)
                                                     tCCACCCGCGGCATTGTCACCGTATGCGTCACTCCGTGGCTTGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACg                                                                                           <  1:810162/148‑1 (MQ=255)
                                                     tCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCCGAGCCGCCGCCAGTGCATCGAAAAAACGCCCACACg                                                                                           <  1:2468615/148‑1 (MQ=255)
                                                      ccACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGa                                                                                          <  2:3310718/148‑1 (MQ=255)
                                                        aCCCGCGGCATTGTCACGGGTTGCGTAAGTCGGTGGCTTGAGGCTGCTAGCGCATCCAGCGCACAAGCCGCTTCACCTTCATAACTTAATGTGGCTGGCGCACAGCCAAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATg                                                                                        <  1:2116303/148‑1 (MQ=255)
                                                        aCCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATg                                                                                        <  2:254246/148‑1 (MQ=255)
                                                        aCCCGCGGAATTGACACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAGGTGGCTGGCGCACAGCCCAGTGCCGCCGCAACTGCATCGAAAAAACGCCCACACGATg                                                                                        <  1:866474/148‑1 (MQ=255)
                                                         cccGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGa                                                                                       >  2:561295/1‑148 (MQ=255)
                                                         cccGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGa                                                                                       >  2:561342/1‑148 (MQ=255)
                                                            gcgGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGc                                                                                    <  1:535816/148‑1 (MQ=255)
                                                            gcgGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGc                                                                                    <  2:699073/148‑1 (MQ=255)
                                                                cATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACTCCCACACGATGACGCCAgc                                                                                >  1:486063/1‑148 (MQ=255)
                                                                   tGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAgcggc                                                                             >  2:1977916/1‑148 (MQ=255)
                                                                     tCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGgcgc                                                                           >  1:2581385/1‑148 (MQ=255)
                                                                      cACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGATGCCAGCGGcgcg                                                                          <  2:1033671/148‑1 (MQ=255)
                                                                      cACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGcgcg                                                                          >  2:1132406/1‑148 (MQ=255)
                                                                         cGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTa                                                                       >  2:721131/1‑148 (MQ=255)
                                                                             tGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTc                                                                   >  2:950226/1‑148 (MQ=255)
                                                                              gCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAGCGCCCACACGATGACGCCAGCGGCGCGTTAATTcc                                                                  <  2:815176/148‑1 (MQ=255)
                                                                              gCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTcc                                                                  <  2:3262161/148‑1 (MQ=255)
                                                                               cGTCACTCGGGGGCTTGTGGCTGCGAGGGCCTCCAGAGCAAACGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCTAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCa                                                                 <  1:1155404/148‑1 (MQ=255)
                                                                                    cTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTc                                                            >  2:2184748/1‑148 (MQ=255)
                                                                                     tCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCa                                                           <  2:987662/148‑1 (MQ=255)
                                                                                     tCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCa                                                           <  2:987265/148‑1 (MQ=255)
                                                                                      ccGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCaa                                                          <  2:403768/148‑1 (MQ=255)
                                                                                      ccGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCaa                                                          >  2:2247049/1‑148 (MQ=255)
                                                                                       cGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAAt                                                         <  2:2841667/148‑1 (MQ=255)
                                                                                       cGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAAt                                                         <  2:2094976/148‑1 (MQ=255)
                                                                                        gTGGAATGCGGCTGCGAGCGCCTCAAGAGCACAAGCCGCTTCACCTTAATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATg                                                        <  1:3122971/148‑1 (MQ=255)
                                                                                         tGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATgg                                                       <  2:1298596/148‑1 (MQ=255)
                                                                                         tGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATgg                                                       <  2:2101701/148‑1 (MQ=255)
                                                                                               gAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCcgcg                                                 >  1:702992/1‑148 (MQ=255)
                                                                                               gAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCCTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCcgcg                                                 >  1:1107051/1‑148 (MQ=255)
                                                                                                aGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACCGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCgcgc                                                <  2:1739093/148‑1 (MQ=255)
                                                                                                   cTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAg                                             <  2:231772/148‑1 (MQ=255)
                                                                                                    tGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGGGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGc                                            <  2:3029072/148‑1 (MQ=255)
                                                                                                    tGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGc                                            <  2:3028910/148‑1 (MQ=255)
                                                                                                     gCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCa                                           <  1:2489409/148‑1 (MQ=255)
                                                                                                       gAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACg                                         <  2:2592210/148‑1 (MQ=255)
                                                                                                          cgcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTc                                      <  1:1244665/148‑1 (MQ=255)
                                                                                                          cgcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTc                                      <  2:2141747/148‑1 (MQ=255)
                                                                                                          cgcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGGGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTc                                      <  2:2226903/148‑1 (MQ=255)
                                                                                                           gcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTcc                                     <  2:1196445/148‑1 (MQ=255)
                                                                                                           gcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTcc                                     <  2:1862068/148‑1 (MQ=255)
                                                                                                            ccTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCa                                    >  1:1547103/1‑148 (MQ=255)
                                                                                                             cgccAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAg                                   >  2:1818719/3‑148 (MQ=255)
                                                                                                             cTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAg                                   <  2:1667673/148‑1 (MQ=255)
                                                                                                                cAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCTAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGttt                                <  1:2254654/148‑1 (MQ=255)
                                                                                                                       caAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGtt                         >  2:1273023/1‑148 (MQ=255)
                                                                                                                          gCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGca                      >  1:1824896/1‑148 (MQ=255)
                                                                                                                           ccGCTTCACCTTCATAACTTAAAGTTGCTGTCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATTGCCCGAGCCAGCACGCACCAGTTTTGCTGTTGcaa                     >  1:30860/1‑147 (MQ=255)
                                                                                                                           ccGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGcac                     >  1:1706521/1‑148 (MQ=255)
                                                                                                                            cGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGcaca                    <  2:1125454/148‑1 (MQ=255)
                                                                                                                             gCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCacac                   <  2:1547103/148‑1 (MQ=255)
                                                                                                                             gCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCacac                   >  2:672177/1‑148 (MQ=255)
                                                                                                                              cTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACt                  <  2:2286691/148‑1 (MQ=255)
                                                                                                                                tCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTg                >  1:1371637/1‑148 (MQ=255)
                                                                                                                                 cACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGc               <  1:1862517/148‑1 (MQ=255)
                                                                                                                                  aCCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCt              >  2:2688541/1‑148 (MQ=255)
                                                                                                                                   ccTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTg             >  2:511544/1‑148 (MQ=255)
                                                                                                                                   ccTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTg             <  2:773365/148‑1 (MQ=255)
                                                                                                                                    cTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGt            <  2:492183/148‑1 (MQ=255)
                                                                                                                                      tCATAACTTAACGGGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGttt          <  1:1232437/148‑1 (MQ=255)
                                                                                                                                         tAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCag       >  1:639827/1‑148 (MQ=255)
                                                                                                                                         tAACTGAAAGTGGCTGGCGCACAGCCCAGGGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGATAATTCCACGCTCACTGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCag       <  2:1877213/148‑1 (MQ=255)
                                                                                                                                           aCTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCagag     <  1:98066/148‑1 (MQ=255)
                                                                                                                                            cTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGt    >  2:627648/1‑148 (MQ=255)
                                                                                                                                            cTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGt    >  2:627642/1‑148 (MQ=255)
                                                                                                                                             ttAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTa   >  1:2798593/1‑148 (MQ=255)
                                                                                                                                              tAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTaa  <  2:54643/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAA  >  CP009273/2828807‑2829096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGGTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCT  >  CP009273/2828804‑2829100
                                                                                                                                                    |                                                                                                                                                    
CCGGTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAG                                                                                                                                                     >  SRR19973070.1357987/1‑150 (MQ=60)
 CGGTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGT                                                                                                                                                    <  SRR19973070.764336/150‑1 (MQ=60)
   GTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGG                                                                                                                                                  <  SRR19973070.3281720/150‑1 (MQ=60)
    TGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGC                                                                                                                                                 <  SRR19973070.2355175/150‑1 (MQ=60)
     GCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCT                                                                                                                                                <  SRR19973070.336799/150‑1 (MQ=60)
       CTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGG                                                                                                                                              >  SRR19973070.2556974/1‑150 (MQ=60)
       CTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGG                                                                                                                                              >  SRR19973070.348309/1‑150 (MQ=60)
       CTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGG                                                                                                                                              <  SRR19973070.1319333/150‑1 (MQ=60)
         GCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACAGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCG                                                                                                                                            >  SRR19973070.1889995/1‑150 (MQ=60)
             GTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACA                                                                                                                                        >  SRR19973070.2115806/1‑150 (MQ=60)
                    CACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGT                                                                                                                                 <  SRR19973070.1442338/150‑1 (MQ=60)
                    CACTGCTGCCAGAAAGTAGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGT                                                                                                                                 >  SRR19973070.778784/1‑150 (MQ=60)
                          TGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCC                                                                                                                           <  SRR19973070.3245524/150‑1 (MQ=60)
                          TGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGACTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCC                                                                                                                           <  SRR19973070.1474779/150‑1 (MQ=60)
                          TGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGACTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCC                                                                                                                           <  SRR19973070.1474661/150‑1 (MQ=60)
                             CAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCC                                                                                                                        >  SRR19973070.1651569/1‑150 (MQ=60)
                               GAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCAC                                                                                                                      >  SRR19973070.1947478/1‑150 (MQ=60)
                                AAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACT                                                                                                                     >  SRR19973070.55286/1‑150 (MQ=60)
                                  AGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGC                                                                                                                   >  SRR19973070.1283224/1‑150 (MQ=60)
                                  AGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGC                                                                                                                   >  SRR19973070.2943033/1‑150 (MQ=60)
                                    TGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCAT                                                                                                                 <  SRR19973070.1542995/150‑1 (MQ=60)
                                    TGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCAT                                                                                                                 <  SRR19973070.1246686/150‑1 (MQ=60)
                                    TGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCAT                                                                                                                 <  SRR19973070.1633483/150‑1 (MQ=60)
                                    GGGCGAGATCCAGGTGATGGTCCACCCGCGGCATTGTCACCGGTTGCGTCACGCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCGGGCGCACAGCCCATAGCCGCCGCCACTGCAT                                                                                                                 <  SRR19973070.1246252/150‑1 (MQ=60)
                                       CGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGA                                                                                                              >  SRR19973070.1373261/1‑150 (MQ=60)
                                       CGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGA                                                                                                              >  SRR19973070.1373249/1‑150 (MQ=60)
                                       CGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCGTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGA                                                                                                              >  SRR19973070.1369873/1‑150 (MQ=60)
                                         AGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAA                                                                                                            <  SRR19973070.2040859/150‑1 (MQ=60)
                                         AGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAA                                                                                                            <  SRR19973070.2744320/150‑1 (MQ=60)
                                           ATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAA                                                                                                          >  SRR19973070.2857761/1‑150 (MQ=60)
                                           ATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAA                                                                                                          >  SRR19973070.1194410/1‑150 (MQ=60)
                                            TCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAA                                                                                                         <  SRR19973070.1844278/150‑1 (MQ=60)
                                              CAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACG                                                                                                       <  SRR19973070.1632112/150‑1 (MQ=60)
                                               AGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGC                                                                                                      >  SRR19973070.790007/1‑150 (MQ=60)
                                                 TTGTTTGTCCACCGGCGGATTTGTCACCGTTTGCTTCACTCCGTGGAATGTGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCTAAAAAACGCCC                                                                                                    <  SRR19973070.991601/150‑1 (MQ=60)
                                                  TGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAATTGGCTTGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCA                                                                                                   >  SRR19973070.1751123/1‑150 (MQ=60)
                                                  TGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAACAAACGCCCA                                                                                                   >  SRR19973070.1751149/1‑150 (MQ=60)
                                                  TGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCA                                                                                                   >  SRR19973070.1751117/1‑150 (MQ=60)
                                                        TCCACCCGCGGCTTTGTCACCGTATGCGTCACTCCGTGGCTTGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGAT                                                                                             <  SRR19973070.815713/150‑1 (MQ=60)
                                                        TCCACCCGCGGCATTGTCACGGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCCGAGCCGCCGCCAGTGCATCGAAAAAACGCCCACACGAT                                                                                             <  SRR19973070.2484715/150‑1 (MQ=60)
                                                         CCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATG                                                                                            <  SRR19973070.1920397/150‑1 (MQ=60)
                                                           ACCCGCGGCATTGTCACGGGTTGCGTAAGTCGGTGGCTTGAGGCTGCTAGCGCATCCAGCGCACAAGCCGCTTCACCTTCATAACTTAATGTGGCTGGCGCACAGCCAAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGAC                                                                                          <  SRR19973070.2130489/150‑1 (MQ=60)
                                                           ACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGAC                                                                                          <  SRR19973070.2130464/150‑1 (MQ=60)
                                                           ACCCGCGGAATTGACACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAGGTGGCTGGCGCACAGCCCAGTGCCGCCGCAACTGCATCGAAAAAACGCCCACACGATGAC                                                                                          <  SRR19973070.872524/150‑1 (MQ=60)
                                                            CCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACG                                                                                         >  SRR19973070.3258169/1‑150 (MQ=60)
                                                               GCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCA                                                                                      <  SRR19973070.539412/150‑1 (MQ=60)
                                                               GCGGAATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCA                                                                                      <  SRR19973070.691921/150‑1 (MQ=60)
                                                                 GGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACTCCCACACGATGACGCCAGC                                                                                    >  SRR19973070.489485/1‑150 (MQ=60)
                                                                      TGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGC                                                                               >  SRR19973070.2598257/1‑150 (MQ=60)
                                                                      TGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGC                                                                               >  SRR19973070.2598256/1‑150 (MQ=60)
                                                                         CACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTT                                                                            >  SRR19973070.1499316/1‑150 (MQ=60)
                                                                                 GCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCAC                                                                    <  SRR19973070.1701041/150‑1 (MQ=60)
                                                                                  CGTCACTCGGGGGCTTGTGGCTGCGAGGGCCTCCAGAGCAAACGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCTAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACG                                                                   <  SRR19973070.1163469/150‑1 (MQ=60)
                                                                                           GTGGCTTGAGGCTGCGAGCGCCTCAAGAGCACATGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGC                                                          <  SRR19973070.3144469/150‑1 (MQ=60)
                                                                                           GTGGAATGCGGCTGCGAGCGCCTCAAGAGCACAAGCCGCTTCACCTTAATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGC                                                          <  SRR19973070.3143129/150‑1 (MQ=60)
                                                                                                ATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCG                                                     >  SRR19973070.708000/1‑150 (MQ=60)
                                                                                                ATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCCTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCG                                                     >  SRR19973070.1114635/1‑150 (MQ=60)
                                                                                                 TGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGC                                                    >  SRR19973070.3106957/1‑150 (MQ=60)
                                                                                                   AGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCA                                                  >  SRR19973070.55574/1‑150 (MQ=60)
                                                                                                        GCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACG                                             <  SRR19973070.2505574/150‑1 (MQ=60)
                                                                                                             CGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCA                                        <  SRR19973070.1253389/150‑1 (MQ=60)
                                                                                                             CGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCA                                        >  SRR19973070.1557985/1‑150 (MQ=60)
                                                                                                             CGCCTCCAGAGCACAAGCCGCTTCACATTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCA                                        <  SRR19973070.1253580/150‑1 (MQ=60)
                                                                                                                CTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTT                                     <  SRR19973070.79801/150‑1 (MQ=60)
                                                                                                                CTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTT                                     <  SRR19973070.1800989/150‑1 (MQ=60)
                                                                                                                CTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAAGTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTT                                     <  SRR19973070.2510844/150‑1 (MQ=60)
                                                                                                                CTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTT                                     >  SRR19973070.352416/1‑150 (MQ=60)
                                                                                                                   CAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCTAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTG                                  <  SRR19973070.2269604/150‑1 (MQ=60)
                                                                                                                           AAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCA                          >  SRR19973070.1837450/1‑150 (MQ=60)
                                                                                                                            AGCCGCTTCACCTTCATAACTTAAAGTTGCTGTCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATTGCCCGAGCCAGCACGCACCAGTTTTGCTGTTGCAA                         >  SRR19973070.31269/1‑150 (MQ=60)
                                                                                                                            AGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCAC                         <  SRR19973070.2161840/150‑1 (MQ=60)
                                                                                                                            AGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCAC                         >  SRR19973070.1718298/1‑150 (MQ=60)
                                                                                                                               CGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACCCCCACACGATGACGCCAGCGGCGCGTTAATTCCAATCTCAAACGCCCGCGCCAGCACCCGCCAGTTATGCATTTGCACACT                      >  SRR19973070.1550440/1‑150 (MQ=60)
                                                                                                                                GCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTT                     <  SRR19973070.1647919/150‑1 (MQ=60)
                                                                                                                                 CTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTG                    >  SRR19973070.1380964/1‑150 (MQ=60)
                                                                                                                                 CTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTG                    >  SRR19973070.1380082/1‑150 (MQ=60)
                                                                                                                                    CACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTG                 <  SRR19973070.1875254/150‑1 (MQ=60)
                                                                                                                                     ACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGT                >  SRR19973070.2750170/1‑150 (MQ=60)
                                                                                                                                       CTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTT              <  SRR19973070.2501931/150‑1 (MQ=60)
                                                                                                                                         TCATAACTTAACGGGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCA            <  SRR19973070.1241068/150‑1 (MQ=60)
                                                                                                                                         TCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCA            <  SRR19973070.1241123/150‑1 (MQ=60)
                                                                                                                                          CATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAG           >  SRR19973070.644386/1‑150 (MQ=60)
                                                                                                                                              ACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTA       >  SRR19973070.2816657/1‑150 (MQ=60)
                                                                                                                                              ACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTA       >  SRR19973070.87267/1‑150 (MQ=60)
                                                                                                                                              ACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTA       >  SRR19973070.87932/1‑150 (MQ=60)
                                                                                                                                              ACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTA       <  SRR19973070.99021/150‑1 (MQ=60)
                                                                                                                                               CTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAA      >  SRR19973070.2997888/1‑150 (MQ=60)
                                                                                                                                                 TAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATT    <  SRR19973070.548614/150‑1 (MQ=60)
                                                                                                                                                  AAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTC   >  SRR19973070.586893/1‑150 (MQ=60)
                                                                                                                                                  AAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTC   >  SRR19973070.586905/1‑150 (MQ=60)
                                                                                                                                                  AAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTC   <  SRR19973070.1400637/150‑1 (MQ=60)
                                                                                                                                                   AAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCT  <  SRR19973070.1565540/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
CCGGTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCT  >  CP009273/2828804‑2829100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑