Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,828,952 C→A 100% T611T (ACG→ACT)  hypF ← carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,828,9520CA99.0% 850.0 / ‑1.3 262T611T (ACG→ACT) hypFcarbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base A (112/143);  total (113/149)
Fisher's exact test for biased strand distribution p-value = 6.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 262 total aligned reads displayed.

GGTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATT  >  CP009273/2828806‑2829098
                                                                                                                                                  |                                                                                                                                                  
ggTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtg                                                                                                                                                 >  1:3466060/1‑150 (MQ=255)
ggTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtg                                                                                                                                                 <  1:554459/150‑1 (MQ=255)
 gTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtgg                                                                                                                                                <  2:4100360/150‑1 (MQ=255)
 gTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtgg                                                                                                                                                >  2:1141419/1‑150 (MQ=255)
   gcctgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggct                                                                                                                                              >  1:4028215/1‑150 (MQ=255)
   gcctgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggct                                                                                                                                              >  2:869999/1‑150 (MQ=255)
    cctgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggctg                                                                                                                                             >  1:1013830/1‑150 (MQ=255)
    cctgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggctg                                                                                                                                             <  2:3420855/150‑1 (MQ=255)
    cctgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggctg                                                                                                                                             >  1:2988279/1‑150 (MQ=255)
      tgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggctggc                                                                                                                                           >  1:1181065/1‑150 (MQ=255)
       gccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGgcg                                                                                                                                          <  1:3725708/150‑1 (MQ=255)
       gccAGTTCAGCCACTGCTGCCAGAAAGGGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGgcg                                                                                                                                          <  2:3359193/150‑1 (MQ=255)
         cAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGca                                                                                                                                        <  2:1658052/150‑1 (MQ=255)
         cAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTGCACCTTCAGAACTTAAAGTGGCTGGCGca                                                                                                                                        >  1:3196683/1‑150 (MQ=255)
          aGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGcac                                                                                                                                       <  1:3266085/150‑1 (MQ=255)
              cAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACTTTCATAACTTAAAGTGGCTGGCGCACAGcc                                                                                                                                   <  1:3861785/150‑1 (MQ=255)
              cAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGcc                                                                                                                                   >  1:44875/1‑150 (MQ=255)
                gCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCa                                                                                                                                 >  1:3892883/1‑150 (MQ=255)
                gCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCa                                                                                                                                 >  1:3432764/1‑150 (MQ=255)
                 ccACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAg                                                                                                                                <  1:1780407/150‑1 (MQ=255)
                  cACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGt                                                                                                                               >  2:3717641/1‑150 (MQ=255)
                  cACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGt                                                                                                                               <  2:1733956/150‑1 (MQ=255)
                  cACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGt                                                                                                                               >  1:2137161/1‑150 (MQ=255)
                     tgctgcCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTgcc                                                                                                                            >  1:872696/1‑150 (MQ=255)
                        tgcCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTgccgcc                                                                                                                         <  1:2256587/150‑1 (MQ=255)
                            aGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCa                                                                                                                     >  1:3953121/1‑150 (MQ=255)
                            aGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCa                                                                                                                     >  2:559979/1‑150 (MQ=255)
                                  tGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCAt                                                                                                               >  2:2810175/1‑150 (MQ=255)
                                    gCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCg                                                                                                             <  2:2348686/150‑1 (MQ=255)
                                        gaTCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGaaaa                                                                                                         >  1:3191779/1‑150 (MQ=255)
                                        gaTCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGaaaa                                                                                                         >  1:2523026/1‑150 (MQ=255)
                                        gaTCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGaaaa                                                                                                         >  2:1290705/1‑150 (MQ=255)
                                         aTCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGaaaaa                                                                                                        >  1:4018196/1‑150 (MQ=255)
                                           ccAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAAc                                                                                                      >  2:755816/1‑150 (MQ=255)
                                             aGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGc                                                                                                    <  2:3561284/150‑1 (MQ=255)
                                              gTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGcc                                                                                                   <  2:1320394/150‑1 (MQ=255)
                                                tGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCca                                                                                                 >  2:2173664/1‑150 (MQ=255)
                                                  aTTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCcaca                                                                                               <  1:2231710/150‑1 (MQ=255)
                                                       ccACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATg                                                                                          >  1:1419627/1‑150 (MQ=255)
                                                        cACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGa                                                                                         >  2:3247167/1‑150 (MQ=255)
                                                        cACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGa                                                                                         <  2:3976804/150‑1 (MQ=255)
                                                        cACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGa                                                                                         <  2:128298/150‑1 (MQ=255)
                                                            cgcgGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGcc                                                                                     >  1:579548/1‑150 (MQ=255)
                                                            cgcgGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGcc                                                                                     <  2:509723/150‑1 (MQ=255)
                                                             gcgGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCa                                                                                    <  1:2487420/150‑1 (MQ=255)
                                                                   ttGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAgcggcg                                                                              <  1:4116346/150‑1 (MQ=255)
                                                                   ttGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAgcggcg                                                                              >  1:967604/1‑150 (MQ=255)
                                                                     gTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGcgcg                                                                            >  1:976407/1‑150 (MQ=255)
                                                                        aCCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTa                                                                         <  2:2479954/150‑1 (MQ=255)
                                                                          cGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATACCTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGACACAACGCCCACACGATGACGCCAGCGGCGCGTTAAt                                                                       <  2:407552/150‑1 (MQ=255)
                                                                              tGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCa                                                                   <  1:3557573/150‑1 (MQ=255)
                                                                              tGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCa                                                                   <  2:1729216/150‑1 (MQ=255)
                                                                                cGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACg                                                                 >  2:1117777/1‑150 (MQ=255)
                                                                                   cACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTc                                                              >  2:347471/1‑150 (MQ=255)
                                                                                       ccGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATg                                                          <  1:153814/150‑1 (MQ=255)
                                                                                        cGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCCCTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATgg                                                         >  2:49288/1‑150 (MQ=255)
                                                                                        cGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACACCAGCGGCGCGTTAATTCCACGCTCAATgg                                                         <  2:3133169/150‑1 (MQ=255)
                                                                                           ggCATGAGGCTGCGAGCGCCTCCATAGCACAAGCCGCTTCACCTACATAACTTAATGGGGCTGGCGCACAGCCCATTGCCGCCGCCAATGCATCTAAAATACGCCATCACGATGACGCCAGCGGCGAGTTAATTCCACGCTCAATGGccc                                                      <  2:4025791/150‑1 (MQ=255)
                                                                                              aTGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCcgcg                                                   >  1:1877432/1‑150 (MQ=255)
                                                                                              aTGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGGTAATTCCACGCTCAATGGCCcgcg                                                   >  2:3535846/1‑150 (MQ=255)
                                                                                                  ggCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAg                                               <  2:226334/150‑1 (MQ=255)
                                                                                                    cTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCa                                             <  2:704340/150‑1 (MQ=255)
                                                                                                     tGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCAc                                            <  1:604078/150‑1 (MQ=255)
                                                                                                     tGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCAc                                            <  1:2065449/150‑1 (MQ=255)
                                                                                                       cGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGc                                          >  2:1179273/1‑150 (MQ=255)
                                                                                                        gAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCt                                         >  1:4136185/1‑150 (MQ=255)
                                                                                                        gAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCt                                         >  1:411181/1‑150 (MQ=255)
                                                                                                        gAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCt                                         >  1:411133/1‑150 (MQ=255)
                                                                                                         aGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAATGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTc                                        <  1:1392770/150‑1 (MQ=255)
                                                                                                          gcgcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTcc                                       <  2:194200/150‑1 (MQ=255)
                                                                                                           cgcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCa                                      <  2:457303/150‑1 (MQ=255)
                                                                                                            gcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGTCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGAAGACGACAGCGGCGCGTTAATTCCACGCACAATGTACCTCGCCAGCACGATCCAg                                     >  2:1053779/1‑150 (MQ=255)
                                                                                                             ccTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGt                                    >  1:2769320/1‑150 (MQ=255)
                                                                                                                ccAGAGCACAAGCCGCTTCACCTTCATACCTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGtttt                                 <  2:891392/150‑1 (MQ=255)
                                                                                                                ccAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGtttt                                 <  2:1537817/150‑1 (MQ=255)
                                                                                                                ccAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGGGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGtttt                                 <  1:710554/150‑1 (MQ=255)
                                                                                                                  agagCACAAGCCGCTTCACCTTCATACCTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGc                               <  2:2832840/150‑1 (MQ=255)
                                                                                                                   gagCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCt                              <  1:2429924/150‑1 (MQ=255)
                                                                                                                       acaAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTg                          <  1:1211786/150‑1 (MQ=255)
                                                                                                                           gCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGcaca                      <  2:3379081/150‑1 (MQ=255)
                                                                                                                            ccGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCacac                     >  1:1941411/1‑150 (MQ=255)
                                                                                                                            ccGCTTAACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCacac                     >  2:2723608/1‑150 (MQ=255)
                                                                                                                              gCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACtt                   <  1:1460246/150‑1 (MQ=255)
                                                                                                                              gCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACtt                   <  2:3797223/150‑1 (MQ=255)
                                                                                                                               cTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTg                  >  2:2079401/1‑150 (MQ=255)
                                                                                                                                ttCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGc                 <  2:1477429/150‑1 (MQ=255)
                                                                                                                                   aCCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTAGCACACTTGCTGt              >  2:2929266/1‑150 (MQ=255)
                                                                                                                                    ccTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCACACACGATGACGCCAGCGGCGCGTTAATACCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGtt             >  2:3816059/1‑150 (MQ=255)
                                                                                                                                      ttCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTc           >  1:1715659/1‑150 (MQ=255)
                                                                                                                                       tCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCa          <  2:1122103/150‑1 (MQ=255)
                                                                                                                                       tCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCa          >  2:3551880/1‑150 (MQ=255)
                                                                                                                                        cATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCag         <  1:1042467/150‑1 (MQ=255)
                                                                                                                                         aTAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCaga        <  2:1452080/150‑1 (MQ=255)
                                                                                                                                          tAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCagag       >  1:472038/1‑150 (MQ=255)
                                                                                                                                           aaCTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGt      <  2:3513488/150‑1 (MQ=255)
                                                                                                                                            aCTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACTCGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTa     <  1:3561303/150‑1 (MQ=255)
                                                                                                                                            aCTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTa     >  2:2615461/1‑150 (MQ=255)
                                                                                                                                             cTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTaa    >  1:2895833/1‑150 (MQ=255)
                                                                                                                                              ttAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAAt   <  1:144812/150‑1 (MQ=255)
                                                                                                                                               tAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAAtt  <  2:3701262/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATT  >  CP009273/2828806‑2829098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTG  >  CP009273/2828806‑2829101
                                                                                                                                                  |                                                                                                                                                     
GGTGCCTGCCAGTTCAGCCACTGCTGCCAGACAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTG                                                                                                                                                    <  SRR19973074.1758927/150‑1 (MQ=60)
GGTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTG                                                                                                                                                    >  SRR19973074.3472428/1‑150 (MQ=60)
GGTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTG                                                                                                                                                    <  SRR19973074.1758718/150‑1 (MQ=60)
GGTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTG                                                                                                                                                    <  SRR19973074.555552/150‑1 (MQ=60)
GGTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTG                                                                                                                                                    <  SRR19973074.555603/150‑1 (MQ=60)
 GTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGG                                                                                                                                                   <  SRR19973074.2459628/150‑1 (MQ=60)
   GCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCT                                                                                                                                                 >  SRR19973074.4035660/1‑150 (MQ=60)
   GCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCT                                                                                                                                                 >  SRR19973074.4035773/1‑150 (MQ=60)
    CCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTG                                                                                                                                                >  SRR19973074.1015713/1‑150 (MQ=60)
    CCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTG                                                                                                                                                <  SRR19973074.3986802/150‑1 (MQ=60)
    CCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTG                                                                                                                                                <  SRR19973074.3986857/150‑1 (MQ=60)
    CCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTG                                                                                                                                                >  SRR19973074.2993838/1‑150 (MQ=60)
    CCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTG                                                                                                                                                >  SRR19973074.2993835/1‑150 (MQ=60)
    CCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTG                                                                                                                                                <  SRR19973074.4091352/150‑1 (MQ=60)
    CCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTG                                                                                                                                                <  SRR19973074.1395835/150‑1 (MQ=60)
     CTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGG                                                                                                                                               <  SRR19973074.2391240/150‑1 (MQ=60)
      TGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGC                                                                                                                                              >  SRR19973074.1183318/1‑150 (MQ=60)
       GCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCG                                                                                                                                             <  SRR19973074.3732691/150‑1 (MQ=60)
       GCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCG                                                                                                                                             <  SRR19973074.3732578/150‑1 (MQ=60)
         CAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTGCACCTTCAGAACTTAAAGTGGCTGGCGCA                                                                                                                                           >  SRR19973074.3202577/1‑150 (MQ=60)
          AGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCAC                                                                                                                                          <  SRR19973074.3272130/150‑1 (MQ=60)
          AGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCAC                                                                                                                                          <  SRR19973074.3271870/150‑1 (MQ=60)
              CAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACTTTCATAACTTAAAGTGGCTGGCGCACAGCC                                                                                                                                      <  SRR19973074.3868835/150‑1 (MQ=60)
              CAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACTTTCATAACTTAAAGTGGCTGGCGCACAGCC                                                                                                                                      <  SRR19973074.3868841/150‑1 (MQ=60)
              CAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCC                                                                                                                                      >  SRR19973074.45003/1‑150 (MQ=60)
                GCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCA                                                                                                                                    >  SRR19973074.3439090/1‑150 (MQ=60)
                GCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCA                                                                                                                                    >  SRR19973074.3900130/1‑150 (MQ=60)
                GCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCA                                                                                                                                    >  SRR19973074.3900015/1‑150 (MQ=60)
                 CCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAG                                                                                                                                   >  SRR19973074.3571163/1‑150 (MQ=60)
                 CCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAG                                                                                                                                   <  SRR19973074.1783769/150‑1 (MQ=60)
                  CACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGT                                                                                                                                  <  SRR19973074.2030652/150‑1 (MQ=60)
                  CACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGT                                                                                                                                  >  SRR19973074.2140955/1‑150 (MQ=60)
                  CACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGT                                                                                                                                  >  SRR19973074.2141173/1‑150 (MQ=60)
                   ACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTG                                                                                                                                 >  SRR19973074.3900394/1‑150 (MQ=60)
                    CTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGC                                                                                                                                >  SRR19973074.1094473/1‑150 (MQ=60)
                    CTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGC                                                                                                                                >  SRR19973074.1214504/1‑150 (MQ=60)
                     TGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCC                                                                                                                               >  SRR19973074.874378/1‑150 (MQ=60)
                        TGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCC                                                                                                                            <  SRR19973074.1885308/150‑1 (MQ=60)
                        TGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCC                                                                                                                            <  SRR19973074.2260821/150‑1 (MQ=60)
                            AGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCA                                                                                                                        >  SRR19973074.1230766/1‑150 (MQ=60)
                            AGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCA                                                                                                                        <  SRR19973074.4034322/150‑1 (MQ=60)
                            AGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCA                                                                                                                        >  SRR19973074.3960405/1‑150 (MQ=60)
                              AAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACT                                                                                                                      <  SRR19973074.3232081/150‑1 (MQ=60)
                                        GATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAA                                                                                                            >  SRR19973074.3197659/1‑150 (MQ=60)
                                        GATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAA                                                                                                            <  SRR19973074.2529602/150‑1 (MQ=60)
                                        GATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAA                                                                                                            >  SRR19973074.3197670/1‑150 (MQ=60)
                                        GATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAA                                                                                                            >  SRR19973074.2527739/1‑150 (MQ=60)
                                        GATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAA                                                                                                            >  SRR19973074.2527730/1‑150 (MQ=60)
                                        GATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAA                                                                                                            >  SRR19973074.2527732/1‑150 (MQ=60)
                                         ATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAA                                                                                                           >  SRR19973074.4025625/1‑150 (MQ=60)
                                             AGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGC                                                                                                       >  SRR19973074.2795256/1‑150 (MQ=60)
                                                  ATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACA                                                                                                  <  SRR19973074.2235912/150‑1 (MQ=60)
                                                  ATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACA                                                                                                  <  SRR19973074.2235909/150‑1 (MQ=60)
                                                  ATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACA                                                                                                  >  SRR19973074.18946/1‑150 (MQ=60)
                                                       CCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATG                                                                                             >  SRR19973074.1422336/1‑150 (MQ=60)
                                                       CCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATG                                                                                             >  SRR19973074.1422254/1‑150 (MQ=60)
                                                            CGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCC                                                                                        >  SRR19973074.580673/1‑150 (MQ=60)
                                                            CGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCC                                                                                        >  SRR19973074.372467/1‑150 (MQ=60)
                                                             GCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCA                                                                                       <  SRR19973074.2492038/150‑1 (MQ=60)
                                                              CGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAG                                                                                      >  SRR19973074.3350023/1‑150 (MQ=60)
                                                                TCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCTTAACTTAACGTGGCTGGCGCACAGCCCTGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCG                                                                                    <  SRR19973074.156085/150‑1 (MQ=60)
                                                                GCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCG                                                                                    <  SRR19973074.2206929/150‑1 (MQ=60)
                                                                   TTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCG                                                                                 <  SRR19973074.4123940/150‑1 (MQ=60)
                                                                   TTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCG                                                                                 >  SRR19973074.969428/1‑150 (MQ=60)
                                                                   TTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCG                                                                                 <  SRR19973074.4123912/150‑1 (MQ=60)
                                                                   TTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCG                                                                                 <  SRR19973074.1799598/150‑1 (MQ=60)
                                                                   TTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGAACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCG                                                                                 <  SRR19973074.4123910/150‑1 (MQ=60)
                                                                     GTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCG                                                                               >  SRR19973074.978241/1‑150 (MQ=60)
                                                                              TGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCA                                                                      <  SRR19973074.3564135/150‑1 (MQ=60)
                                                                              TGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCA                                                                      <  SRR19973074.3564083/150‑1 (MQ=60)
                                                                              TGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCA                                                                      <  SRR19973074.3564130/150‑1 (MQ=60)
                                                                               GCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCAC                                                                     <  SRR19973074.3508450/150‑1 (MQ=60)
                                                                                       CCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATG                                                             <  SRR19973074.154161/150‑1 (MQ=60)
                                                                                              ATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCG                                                      >  SRR19973074.1881023/1‑150 (MQ=60)
                                                                                              ATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCG                                                      >  SRR19973074.2898823/1‑150 (MQ=60)
                                                                                                     TGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCAC                                               <  SRR19973074.605223/150‑1 (MQ=60)
                                                                                                     TGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCAC                                               <  SRR19973074.2069325/150‑1 (MQ=60)
                                                                                                     TGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCAC                                               <  SRR19973074.605391/150‑1 (MQ=60)
                                                                                                     TGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCAC                                               <  SRR19973074.605228/150‑1 (MQ=60)
                                                                                                       CGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGC                                             >  SRR19973074.47222/1‑150 (MQ=60)
                                                                                                       CGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGC                                             >  SRR19973074.47288/1‑150 (MQ=60)
                                                                                                        GAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCT                                            >  SRR19973074.1059720/1‑150 (MQ=60)
                                                                                                        GAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCT                                            >  SRR19973074.411918/1‑150 (MQ=60)
                                                                                                        GAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCT                                            >  SRR19973074.411958/1‑150 (MQ=60)
                                                                                                        GAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCT                                            >  SRR19973074.411966/1‑150 (MQ=60)
                                                                                                        GAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCT                                            >  SRR19973074.4143807/1‑150 (MQ=60)
                                                                                                        GAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCT                                            >  SRR19973074.4143815/1‑150 (MQ=60)
                                                                                                         agcgactcccgagaacaatcagattcaaCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGTCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTC                                           <  SRR19973074.993505/122‑1 (MQ=60)
                                                                                                         AGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAATGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTC                                           <  SRR19973074.1395327/150‑1 (MQ=60)
                                                                                                         AGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTC                                           >  SRR19973074.3461056/1‑150 (MQ=60)
                                                                                                         AGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTC                                           <  SRR19973074.3290172/150‑1 (MQ=60)
                                                                                                         AGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTC                                           <  SRR19973074.1192936/150‑1 (MQ=60)
                                                                                                            GCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAG                                        >  SRR19973074.2321400/1‑150 (MQ=60)
                                                                                                             CCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGT                                       >  SRR19973074.2774407/1‑150 (MQ=60)
                                                                                                              CTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTT                                      <  SRR19973074.3752890/150‑1 (MQ=60)
                                                                                                              CTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTT                                      >  SRR19973074.974447/1‑150 (MQ=60)
                                                                                                                CCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGGGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTT                                    <  SRR19973074.711874/150‑1 (MQ=60)
                                                                                                                   GAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCT                                 >  SRR19973074.54372/1‑150 (MQ=60)
                                                                                                                   GAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCT                                 <  SRR19973074.2434418/150‑1 (MQ=60)
                                                                                                                   GAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCT                                 <  SRR19973074.592940/150‑1 (MQ=60)
                                                                                                                       ACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTG                             <  SRR19973074.314328/150‑1 (MQ=60)
                                                                                                                       ACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTG                             <  SRR19973074.1214110/150‑1 (MQ=60)
                                                                                                                           GCCGCTTCACCTTCATAACTTAAAGGGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACA                         <  SRR19973074.476399/150‑1 (MQ=60)
                                                                                                                            CCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACAC                        >  SRR19973074.412857/1‑150 (MQ=60)
                                                                                                                            CCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACAC                        >  SRR19973074.1945122/1‑150 (MQ=60)
                                                                                                                            CCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACAC                        >  SRR19973074.412881/1‑150 (MQ=60)
                                                                                                                             CGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCGCACCCGTTGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCTGCACGCTCCAGTTTTGCTGTTGCACACT                       <  SRR19973074.292167/150‑1 (MQ=60)
                                                                                                                             CGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACT                       <  SRR19973074.1653177/150‑1 (MQ=60)
                                                                                                                              GCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTT                      <  SRR19973074.1462980/150‑1 (MQ=60)
                                                                                                                              GCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTT                      <  SRR19973074.1462944/150‑1 (MQ=60)
                                                                                                                                TTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGC                    <  SRR19973074.1971636/150‑1 (MQ=60)
                                                                                                                                 TCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCT                   >  SRR19973074.736979/1‑150 (MQ=60)
                                                                                                                                    CCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTT                >  SRR19973074.893337/1‑150 (MQ=60)
                                                                                                                                     CTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTT               >  SRR19973074.1518325/1‑150 (MQ=60)
                                                                                                                                      TTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTC              >  SRR19973074.1718847/1‑150 (MQ=60)
                                                                                                                                        CATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAG            <  SRR19973074.1044382/150‑1 (MQ=60)
                                                                                                                                          TAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAG          >  SRR19973074.472968/1‑150 (MQ=60)
                                                                                                                                           AACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCTGAGT         <  SRR19973074.796767/150‑1 (MQ=60)
                                                                                                                                           AACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGT         <  SRR19973074.2173122/150‑1 (MQ=60)
                                                                                                                                           AACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGT         >  SRR19973074.248104/1‑150 (MQ=60)
                                                                                                                                            ACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACTCGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTA        <  SRR19973074.3567865/150‑1 (MQ=60)
                                                                                                                                            ACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTA        <  SRR19973074.925117/150‑1 (MQ=60)
                                                                                                                                             CTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAA       <  SRR19973074.1409246/150‑1 (MQ=60)
                                                                                                                                             CTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAA       >  SRR19973074.2901211/1‑150 (MQ=60)
                                                                                                                                             CTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAA       <  SRR19973074.1043118/150‑1 (MQ=60)
                                                                                                                                              TTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAAT      <  SRR19973074.145141/150‑1 (MQ=60)
                                                                                                                                              TTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAAT      <  SRR19973074.145255/150‑1 (MQ=60)
                                                                                                                                                  AGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTG  <  SRR19973074.631017/150‑1 (MQ=60)
                                                                                                                                                  AGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTG  <  SRR19973074.631016/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
GGTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTG  >  CP009273/2828806‑2829101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑