Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,519,188 C→A 87.2% L107L (CTG→CTT)  nudE ← adenosine nucleotide hydrolase; substrates include Ap3A, Ap2A, ADP‑ribose, NADH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,519,1880CA87.2% 497.4 / 78.7 220L107L (CTG→CTT) nudEadenosine nucleotide hydrolase; substrates include Ap3A, Ap2A, ADP‑ribose, NADH
Reads supporting (aligned to +/- strand):  ref base C (14/14);  new base A (87/105);  total (101/119)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.77e-01

Only 100 of 220 total aligned reads displayed.

TCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGGTG  >  CP009273/3519043‑3519334
                                                                                                                                                 |                                                                                                                                                  
tcatATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAg                                                                                                                                                  <  1:165083/148‑1 (MQ=255)
 catATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGa                                                                                                                                                 >  1:900796/1‑148 (MQ=255)
 catATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGa                                                                                                                                                 <  1:196837/148‑1 (MQ=255)
    atGCGCCCGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCg                                                                                                                                              <  2:856039/148‑1 (MQ=255)
     tGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGt                                                                                                                                             <  2:1088292/148‑1 (MQ=255)
     tGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGt                                                                                                                                             <  2:223222/148‑1 (MQ=255)
      gcgcCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTCGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGtt                                                                                                                                            >  1:3168716/1‑148 (MQ=255)
         ccagcggccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGc                                                                                                                                         >  1:1624603/1‑148 (MQ=255)
           agcggccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTGCAAAAAAGTAAGATCGTTCGCTc                                                                                                                                       >  2:3292694/1‑148 (MQ=255)
            gcggccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTcc                                                                                                                                      >  2:689233/1‑148 (MQ=255)
             cggccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCa                                                                                                                                     >  2:482601/1‑148 (MQ=255)
               gccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCaaa                                                                                                                                   >  2:2624281/1‑148 (MQ=255)
                 cagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATc                                                                                                                                 >  1:1821320/1‑148 (MQ=255)
                   gcgcACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCa                                                                                                                               >  1:2110024/1‑148 (MQ=255)
                    cgcACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGGTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCaa                                                                                                                              <  2:1273627/148‑1 (MQ=255)
                       aCCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACct                                                                                                                           <  1:456156/148‑1 (MQ=255)
                       aCCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACct                                                                                                                           >  1:3106114/1‑148 (MQ=255)
                       aCCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACct                                                                                                                           >  2:1756643/1‑148 (MQ=255)
                        ccTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACctc                                                                                                                          >  2:2172572/1‑148 (MQ=255)
                         cTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACctct                                                                                                                         >  1:795128/1‑148 (MQ=255)
                         cTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACctct                                                                                                                         >  2:1853895/1‑148 (MQ=255)
                           gtgGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCtcttc                                                                                                                       <  1:3245927/148‑1 (MQ=255)
                             ggTAGCGGCTCTGGCTCATCGCCTTCTAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTcttctt                                                                                                                     <  1:374050/148‑1 (MQ=255)
                             ggTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTcttctt                                                                                                                     >  1:2294028/1‑148 (MQ=255)
                              gTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGTAATGTAAGACGGTGCCATGCTGAGGTTCTTCAATATAGTAAGATCGTTCGCTGCAAATCCAACCTGTTCttt                                                                                                                    >  1:656948/1‑148 (MQ=255)
                                aGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTa                                                                                                                  <  1:3050462/148‑1 (MQ=255)
                                aGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTa                                                                                                                  <  1:2160501/148‑1 (MQ=255)
                                aGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTa                                                                                                                  <  2:2330553/148‑1 (MQ=255)
                                 gCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAg                                                                                                                 <  1:20309/148‑1 (MQ=255)
                                    gCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTc                                                                                                              >  2:1547966/1‑148 (MQ=255)
                                     ctctGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTcg                                                                                                             >  1:2150135/1‑148 (MQ=255)
                                     ctctGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTcg                                                                                                             >  2:2521134/1‑148 (MQ=255)
                                     ctctGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTcg                                                                                                             >  1:487594/1‑148 (MQ=255)
                                     ctctGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTcg                                                                                                             <  2:2113386/148‑1 (MQ=255)
                                       ctGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTcgcg                                                                                                           >  2:899396/1‑148 (MQ=255)
                                          gCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGtt                                                                                                        >  2:607521/1‑148 (MQ=255)
                                          gCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGtt                                                                                                        <  1:1291847/148‑1 (MQ=255)
                                          gCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGtt                                                                                                        <  1:746074/148‑1 (MQ=255)
                                               tCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCgg                                                                                                   >  2:511929/1‑148 (MQ=255)
                                                cGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGc                                                                                                  <  1:2197209/148‑1 (MQ=255)
                                                cGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGc                                                                                                  >  2:620706/1‑148 (MQ=255)
                                                 gCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCt                                                                                                 <  1:2520262/148‑1 (MQ=255)
                                                     tCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGt                                                                                             <  2:2600055/148‑1 (MQ=255)
                                                      ccAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTa                                                                                            >  1:1382284/1‑148 (MQ=255)
                                                              tCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCttt                                                                                    >  2:3048652/1‑148 (MQ=255)
                                                                  ggTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCAcc                                                                                <  2:862634/148‑1 (MQ=255)
                                                                        gaTCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATc                                                                          >  1:1288814/1‑148 (MQ=255)
                                                                         aTCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCa                                                                         <  1:979252/148‑1 (MQ=255)
                                                                           cTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAAt                                                                       <  1:1853895/148‑1 (MQ=255)
                                                                                gcTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATc                                                                  <  1:636675/148‑1 (MQ=255)
                                                                                 cTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATcc                                                                 <  2:669771/148‑1 (MQ=255)
                                                                                 cTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATcc                                                                 >  1:2749135/1‑148 (MQ=255)
                                                                                 cTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATcc                                                                 <  2:56081/148‑1 (MQ=255)
                                                                                  tACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCt                                                                >  2:1491332/1‑148 (MQ=255)
                                                                                  tACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCt                                                                <  2:47088/148‑1 (MQ=255)
                                                                                   aCCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCtt                                                               <  2:2190469/148‑1 (MQ=255)
                                                                                    ccACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCttt                                                              >  2:253845/1‑148 (MQ=255)
                                                                                     cACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTc                                                             >  1:812727/1‑148 (MQ=255)
                                                                                      aCGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCg                                                            >  2:2929431/1‑148 (MQ=255)
                                                                                       cGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGa                                                           >  2:150447/1‑148 (MQ=255)
                                                                                         atatTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGaaa                                                         <  1:687966/148‑1 (MQ=255)
                                                                                          tatTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGaaaa                                                        >  2:1439764/1‑148 (MQ=255)
                                                                                            ttCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAAc                                                      <  2:3168716/148‑1 (MQ=255)
                                                                                            ttCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAAc                                                      <  1:1074118/148‑1 (MQ=255)
                                                                                             tCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAAcc                                                     >  2:2681098/1‑148 (MQ=255)
                                                                                             tCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAAcc                                                     <  1:1120984/148‑1 (MQ=255)
                                                                                                ttttGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTaa                                                  >  1:2925237/1‑148 (MQ=255)
                                                                                                ttttGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTaa                                                  >  1:1323639/1‑148 (MQ=255)
                                                                                                ttttGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTaa                                                  >  1:95933/1‑148 (MQ=255)
                                                                                                 tttGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAAt                                                 <  2:20999/148‑1 (MQ=255)
                                                                                                 tttGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCTAAAAACCTAAt                                                 >  2:2164942/1‑148 (MQ=255)
                                                                                                  ttGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAAtt                                                <  2:2514018/148‑1 (MQ=255)
                                                                                                  ttGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAAtt                                                <  2:1947833/148‑1 (MQ=255)
                                                                                                     cTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGt                                             <  1:633970/148‑1 (MQ=255)
                                                                                                     cTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGt                                             <  2:2890432/148‑1 (MQ=255)
                                                                                                      tGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTa                                            >  1:1448545/1‑148 (MQ=255)
                                                                                                         aaaaGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGa                                         <  2:2826971/148‑1 (MQ=255)
                                                                                                          aaaGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGAt                                        >  2:1578358/1‑148 (MQ=255)
                                                                                                           aaGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGAtt                                       >  2:1998191/1‑148 (MQ=255)
                                                                                                           aaGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGAtt                                       <  1:2172572/148‑1 (MQ=255)
                                                                                                            aGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCAAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTc                                      >  2:2273279/1‑148 (MQ=255)
                                                                                                              tAAGACGGTGCCATGCTGAGCCTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAg                                    <  1:119314/148‑1 (MQ=255)
                                                                                                                aGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGtt                                  >  2:3286417/1‑148 (MQ=255)
                                                                                                                 gACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTc                                 <  2:1884150/148‑1 (MQ=255)
                                                                                                                 gACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTc                                 <  2:1692626/148‑1 (MQ=255)
                                                                                                                    ggTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCa                              <  1:564398/148‑1 (MQ=255)
                                                                                                                         cATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCg                         <  2:910873/148‑1 (MQ=255)
                                                                                                                         cATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAAGTCGTAGGATTCAGTTCCCACTGCg                         <  2:2565619/148‑1 (MQ=255)
                                                                                                                          aTGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGt                        <  2:294804/148‑1 (MQ=255)
                                                                                                                          aTGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGt                        >  2:1819800/1‑148 (MQ=255)
                                                                                                                            gCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTAt                      <  2:2702714/148‑1 (MQ=255)
                                                                                                                            gCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTAt                      <  1:2658687/148‑1 (MQ=255)
                                                                                                                                  cttcttCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCgg                >  2:2138443/1‑148 (MQ=255)
                                                                                                                                  cttcttCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCgg                >  1:583556/1‑148 (MQ=255)
                                                                                                                                   ttcttcAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCgga               <  1:511929/148‑1 (MQ=255)
                                                                                                                                   ttcttcAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCgga               >  2:1311206/1‑148 (MQ=255)
                                                                                                                                    tcttcAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCggat              <  1:2709409/148‑1 (MQ=255)
                                                                                                                                    tcttcAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATACAGTTCCCACTGCGTATTCGCggat              >  2:274517/1‑148 (MQ=255)
                                                                                                                                        cAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCggatcagg          <  1:2531428/148‑1 (MQ=255)
                                                                                                                                           aaaaGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCggatcaggatc       <  2:812727/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGGTG  >  CP009273/3519043‑3519334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGGTGATC  >  CP009273/3519039‑3519337
                                                                                                                                                     |                                                                                                                                                     
TCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTA                                                                                                                                                       <  SRR19973070.2541557/150‑1 (MQ=60)
TCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTA                                                                                                                                                       >  SRR19973070.858297/1‑150 (MQ=60)
   ATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGA                                                                                                                                                    >  SRR19973070.907178/1‑150 (MQ=60)
    TCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGAT                                                                                                                                                   <  SRR19973070.166428/150‑1 (MQ=60)
    TCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGAT                                                                                                                                                   <  SRR19973070.665544/150‑1 (MQ=60)
     CATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATC                                                                                                                                                  >  SRR19973070.2708154/1‑150 (MQ=60)
     CATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATC                                                                                                                                                  <  SRR19973070.198340/150‑1 (MQ=60)
        ATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTCGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTT                                                                                                                                               >  SRR19973070.3189219/1‑150 (MQ=60)
           CGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGC                                                                                                                                            >  SRR19973070.1635888/1‑150 (MQ=60)
                   GCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATC                                                                                                                                    >  SRR19973070.1833921/1‑150 (MQ=60)
                   GCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATC                                                                                                                                    >  SRR19973070.1833861/1‑150 (MQ=60)
                     CAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCA                                                                                                                                  >  SRR19973070.2124177/1‑150 (MQ=60)
                       GCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAAC                                                                                                                                <  SRR19973070.2437510/150‑1 (MQ=60)
                         GCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCT                                                                                                                              >  SRR19973070.3126151/1‑150 (MQ=60)
                           ACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCT                                                                                                                            >  SRR19973070.800622/1‑150 (MQ=60)
                           ACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCT                                                                                                                            <  SRR19973070.459427/150‑1 (MQ=60)
                               GTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTT                                                                                                                        <  SRR19973070.3266845/150‑1 (MQ=60)
                               GTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTT                                                                                                                        <  SRR19973070.975614/150‑1 (MQ=60)
                               GTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCACTTCTT                                                                                                                        >  SRR19973070.2309288/1‑150 (MQ=60)
                                TGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGTAATGTAAGACGGTGCCATGCTGAGGTTCTTCAATATAGTAAGATCGTTCGCTGCAAATCCAACCTGTTCTTT                                                                                                                       >  SRR19973070.661629/1‑150 (MQ=60)
                                TGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTT                                                                                                                       >  SRR19973070.661628/1‑150 (MQ=60)
                                 GGTAGCGGCTCTGGCTCATCGCCTTCTAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTT                                                                                                                      <  SRR19973070.376794/150‑1 (MQ=60)
                                    AGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCCACCTCTTCTTTTAGC                                                                                                                   <  SRR19973070.3070433/150‑1 (MQ=60)
                                    AGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGC                                                                                                                   <  SRR19973070.2174861/150‑1 (MQ=60)
                                    AGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGC                                                                                                                   <  SRR19973070.3069893/150‑1 (MQ=60)
                                     GCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCT                                                                                                                  <  SRR19973070.20604/150‑1 (MQ=60)
                                       GGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCG                                                                                                                >  SRR19973070.491018/1‑150 (MQ=60)
                                       GGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCG                                                                                                                >  SRR19973070.2164451/1‑150 (MQ=60)
                                              GCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAG                                                                                                         <  SRR19973070.751372/150‑1 (MQ=60)
                                              GCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAG                                                                                                         <  SRR19973070.2422328/150‑1 (MQ=60)
                                              GCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAG                                                                                                         <  SRR19973070.751344/150‑1 (MQ=60)
                                              GCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAG                                                                                                         <  SRR19973070.1300849/150‑1 (MQ=60)
                                              GCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAG                                                                                                         <  SRR19973070.1300877/150‑1 (MQ=60)
                                              GCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAC                                                                                                         <  SRR19973070.1300841/150‑1 (MQ=60)
                                                    CGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTT                                                                                                   <  SRR19973070.2211705/150‑1 (MQ=60)
                                                     GCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTC                                                                                                  <  SRR19973070.2536694/150‑1 (MQ=60)
                                                     GCCTTCCAGTGAGTCCGGGTAGAGATCTTGCGCTAACACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTC                                                                                                  <  SRR19973070.2537578/150‑1 (MQ=60)
                                                        TTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTA                                                                                               >  SRR19973070.21304/1‑150 (MQ=60)
                                                        TTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTA                                                                                               >  SRR19973070.1391646/1‑150 (MQ=60)
                                                        TTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTA                                                                                               >  SRR19973070.22947/1‑150 (MQ=60)
                                                            AGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACG                                                                                           <  SRR19973070.3181558/150‑1 (MQ=60)
                                                              TGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCT                                                                                         <  SRR19973070.3048590/150‑1 (MQ=60)
                                                                         AGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTATGACGGTGCCAGGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGAT                                                                              <  SRR19973070.3313812/150‑1 (MQ=60)
                                                                         AGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGAT                                                                              <  SRR19973070.3313224/150‑1 (MQ=60)
                                                                         AGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGAT                                                                              >  SRR19973070.51711/1‑150 (MQ=60)
                                                                         AGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCATCTTTTAGCTCGCGGTTAGCGGCTTCGAAGACGCTTTCACACGGAT                                                                              >  SRR19973070.2142768/1‑150 (MQ=60)
                                                                          GAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATC                                                                             >  SRR19973070.1297800/1‑150 (MQ=60)
                                                                             ATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAAT                                                                          <  SRR19973070.986239/150‑1 (MQ=60)
                                                                              TCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATT                                                                         <  SRR19973070.2139251/150‑1 (MQ=60)
                                                                               CTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTA                                                                        <  SRR19973070.1866594/150‑1 (MQ=60)
                                                                                  GCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATC                                                                     <  SRR19973070.2641405/150‑1 (MQ=60)
                                                                                   CGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCC                                                                    >  SRR19973070.1104659/1‑150 (MQ=60)
                                                                                   CGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCC                                                                    >  SRR19973070.2766798/1‑150 (MQ=60)
                                                                                    GCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCT                                                                   <  SRR19973070.641199/150‑1 (MQ=60)
                                                                                    GCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCT                                                                   <  SRR19973070.641206/150‑1 (MQ=60)
                                                                                    GCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCT                                                                   <  SRR19973070.3197906/150‑1 (MQ=60)
                                                                                    GCTAACACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAATTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCT                                                                   <  SRR19973070.1649363/150‑1 (MQ=60)
                                                                                     CTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTT                                                                  >  SRR19973070.749610/1‑150 (MQ=60)
                                                                                       ACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTC                                                                >  SRR19973070.818295/1‑150 (MQ=60)
                                                                                       ACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTC                                                                >  SRR19973070.818294/1‑150 (MQ=60)
                                                                                             ATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAA                                                          <  SRR19973070.692873/150‑1 (MQ=60)
                                                                                             ATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAA                                                          <  SRR19973070.694152/150‑1 (MQ=60)
                                                                                             ATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAA                                                          >  SRR19973070.1164660/1‑150 (MQ=60)
                                                                                              TATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAAC                                                         <  SRR19973070.1784714/150‑1 (MQ=60)
                                                                                                TTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCT                                                       <  SRR19973070.1081607/150‑1 (MQ=60)
                                                                                                 TCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTA                                                      <  SRR19973070.1128657/150‑1 (MQ=60)
                                                                                                  CATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAA                                                     >  SRR19973070.2944122/1‑150 (MQ=60)
                                                                                                  CATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAA                                                     >  SRR19973070.1332789/1‑150 (MQ=60)
                                                                                                  CATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAA                                                     >  SRR19973070.1331984/1‑150 (MQ=60)
                                                                                                  CATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAA                                                     >  SRR19973070.2165222/1‑150 (MQ=60)
                                                                                                  CATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAA                                                     >  SRR19973070.96872/1‑150 (MQ=60)
                                                                                                        GCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTA                                               >  SRR19973070.1458613/1‑150 (MQ=60)
                                                                                                         CTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAG                                              <  SRR19973070.638474/150‑1 (MQ=60)
                                                                                                               AAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCA                                        <  SRR19973070.2186975/150‑1 (MQ=60)
                                                                                                               AAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCA                                        <  SRR19973070.2186964/150‑1 (MQ=60)
                                                                                                                AGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAG                                       <  SRR19973070.2760457/150‑1 (MQ=60)
                                                                                                                  TAAGACGGTGCCATGCTGAGCCTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTT                                     <  SRR19973070.120383/150‑1 (MQ=60)
                                                                                                                   AAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTC                                    >  SRR19973070.1402089/1‑150 (MQ=60)
                                                                                                                   AAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTC                                    >  SRR19973070.860686/1‑150 (MQ=60)
                                                                                                                   AAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTC                                    >  SRR19973070.533495/1‑150 (MQ=60)
                                                                                                                       CGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCAC                                <  SRR19973070.955387/150‑1 (MQ=60)
                                                                                                                        GGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCGCACT                               <  SRR19973070.568180/150‑1 (MQ=60)
                                                                                                                        GGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACT                               <  SRR19973070.568172/150‑1 (MQ=60)
                                                                                                                        GGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACT                               <  SRR19973070.2852058/150‑1 (MQ=60)
                                                                                                                        GGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACT                               <  SRR19973070.2027651/150‑1 (MQ=60)
                                                                                                                             CATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTA                          >  SRR19973070.585404/1‑150 (MQ=60)
                                                                                                                                GCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTC                       <  SRR19973070.2675979/150‑1 (MQ=60)
                                                                                                                                 CTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCG                      <  SRR19973070.418662/150‑1 (MQ=60)
                                                                                                                                   GAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCG                    >  SRR19973070.138245/1‑150 (MQ=60)
                                                                                                                                    AGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGG                   >  SRR19973070.587576/1‑150 (MQ=60)
                                                                                                                                    AGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGG                   >  SRR19973070.587593/1‑150 (MQ=60)
                                                                                                                                       TTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATC                <  SRR19973070.2188814/150‑1 (MQ=60)
                                                                                                                                       TTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATC                <  SRR19973070.515447/150‑1 (MQ=60)
                                                                                                                                       TTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATC                >  SRR19973070.1283480/1‑150 (MQ=60)
                                                                                                                                        TCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCA               <  SRR19973070.1964918/150‑1 (MQ=60)
                                                                                                                                        TCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCA               <  SRR19973070.2726920/150‑1 (MQ=60)
                                                                                                                                            CAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGAT           <  SRR19973070.2547971/150‑1 (MQ=60)
                                                                                                                                             AAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATC          <  SRR19973070.2928694/150‑1 (MQ=60)
                                                                                                                                             AAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATC          <  SRR19973070.1589429/150‑1 (MQ=60)
                                                                                                                                               AAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAG        <  SRR19973070.1704545/150‑1 (MQ=60)
                                                                                                                                                AAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGG       >  SRR19973070.967397/1‑150 (MQ=60)
                                                                                                                                                  AGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGGTG     >  SRR19973070.2894771/1‑150 (MQ=60)
                                                                                                                                                  AGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGGTG     >  SRR19973070.1458995/1‑150 (MQ=60)
                                                                                                                                                   GTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGGTGA    <  SRR19973070.1476587/150‑1 (MQ=60)
                                                                                                                                                   GTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGGTGA    <  SRR19973070.1476566/150‑1 (MQ=60)
                                                                                                                                                    TCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGGTGAT   <  SRR19973070.479596/150‑1 (MQ=60)
                                                                                                                                                    TAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGGTGAT   <  SRR19973070.152952/150‑1 (MQ=60)
                                                                                                                                                     AAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGGTGATC  >  SRR19973070.503975/1‑150 (MQ=60)
                                                                                                                                                     AAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGGTGATC  >  SRR19973070.503962/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGGTGATC  >  CP009273/3519039‑3519337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑