Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,519,188 C→A 100% L107L (CTG→CTT)  nudE ← adenosine nucleotide hydrolase; substrates include Ap3A, Ap2A, ADP‑ribose, NADH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,519,1880CA97.8% 752.4 / 5.6 242L107L (CTG→CTT) nudEadenosine nucleotide hydrolase; substrates include Ap3A, Ap2A, ADP‑ribose, NADH
Reads supporting (aligned to +/- strand):  ref base C (1/5);  new base A (114/122);  total (115/127)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 242 total aligned reads displayed.

ATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGG  >  CP009273/3519042‑3519332
                                                                                                                                                  |                                                                                                                                                
 tcatATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAg                                                                                                                                                <  1:2660111/148‑1 (MQ=255)
  catCTGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGa                                                                                                                                               <  1:1942574/148‑1 (MQ=255)
  catATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTACAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGa                                                                                                                                               <  1:1254699/148‑1 (MQ=255)
    tatGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATc                                                                                                                                             >  1:2051327/1‑148 (MQ=255)
      tGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCAATGCTGAGCTTCTTCAAAAAAGTAAGATCGt                                                                                                                                           <  1:1455981/148‑1 (MQ=255)
      tGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCAATGCTGAGCTTCTTCAAAAAAGTAAGATCGt                                                                                                                                           <  1:1456255/148‑1 (MQ=255)
         gccagcggccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCg                                                                                                                                        <  2:2363997/148‑1 (MQ=255)
            agcggccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTc                                                                                                                                     >  1:1635245/1‑148 (MQ=255)
            agcggccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTc                                                                                                                                     >  1:3373911/1‑148 (MQ=255)
            agcggccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTACAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTc                                                                                                                                     >  1:2941758/1‑148 (MQ=255)
              cggccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCa                                                                                                                                   >  2:1592562/1‑148 (MQ=255)
               ggccagcgCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCaa                                                                                                                                  <  1:3158907/148‑1 (MQ=255)
                    gcgcACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCa                                                                                                                             >  2:2838585/1‑148 (MQ=255)
                         ccTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACctc                                                                                                                        >  1:2944552/1‑148 (MQ=255)
                         ccTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACctc                                                                                                                        >  1:2205945/1‑148 (MQ=255)
                         ccTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACctc                                                                                                                        >  1:1069049/1‑148 (MQ=255)
                          cTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACctct                                                                                                                       <  2:504592/148‑1 (MQ=255)
                           tgtgGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCtctt                                                                                                                      <  2:367205/148‑1 (MQ=255)
                            gtgGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCtcttc                                                                                                                     <  2:1428650/148‑1 (MQ=255)
                             tgGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCtcttct                                                                                                                    >  2:3176631/1‑148 (MQ=255)
                               gTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTATAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCttt                                                                                                                  <  2:946500/148‑1 (MQ=255)
                               gTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCttt                                                                                                                  <  2:1766080/148‑1 (MQ=255)
                                  gCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAg                                                                                                               <  2:2447484/148‑1 (MQ=255)
                                      ctctGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCAAAATCCAACCTCTTCTTTTAGCTcg                                                                                                           >  2:1171345/1‑148 (MQ=255)
                                          ggCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGt                                                                                                       <  2:149392/148‑1 (MQ=255)
                                          ggCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGt                                                                                                       <  2:1037188/148‑1 (MQ=255)
                                          ggCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGt                                                                                                       <  2:1301653/148‑1 (MQ=255)
                                           gCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGtt                                                                                                      >  2:2039516/1‑148 (MQ=255)
                                           gCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGtt                                                                                                      >  2:493541/1‑148 (MQ=255)
                                           gCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGtt                                                                                                      >  1:2967892/1‑148 (MQ=255)
                                             tCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAg                                                                                                    <  2:310783/148‑1 (MQ=255)
                                              cATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGc                                                                                                   >  2:1289138/1‑148 (MQ=255)
                                                tCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCgg                                                                                                 <  2:3511857/148‑1 (MQ=255)
                                                 cGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGc                                                                                                <  2:1133282/148‑1 (MQ=255)
                                                  gCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCt                                                                                               <  1:397832/148‑1 (MQ=255)
                                                           tGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGATCGCGGTTAGCGGCTTCGTAGACg                                                                                      <  2:1903502/148‑1 (MQ=255)
                                                            gATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGc                                                                                     <  1:1122433/148‑1 (MQ=255)
                                                              ttCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCtt                                                                                   >  2:766088/1‑148 (MQ=255)
                                                              ttCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCtt                                                                                   <  2:2991737/148‑1 (MQ=255)
                                                               tCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCttt                                                                                  <  1:3084649/148‑1 (MQ=255)
                                                                    gTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCAccc                                                                             >  1:1860912/1‑148 (MQ=255)
                                                                       gagaTCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGa                                                                          <  1:3167902/148‑1 (MQ=255)
                                                                       gagaTCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGa                                                                          <  1:2464409/148‑1 (MQ=255)
                                                                       gagaTCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGa                                                                          <  1:1170259/148‑1 (MQ=255)
                                                                       gagaTCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGa                                                                          <  2:1188691/148‑1 (MQ=255)
                                                                            cTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCg                                                                                                  >  2:154196/1‑119 (MQ=255)
                                                                            cTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCg                                                                                                  <  1:154196/119‑1 (MQ=255)
                                                                              tGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTa                                                                   <  2:2470864/148‑1 (MQ=255)
                                                                                    aCCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCATCCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCtt                                                             <  2:63148/148‑1 (MQ=255)
                                                                                    aCCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCtt                                                             <  2:464413/148‑1 (MQ=255)
                                                                                    aCCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCtt                                                             >  2:1644540/1‑148 (MQ=255)
                                                                                     ccACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCttt                                                            <  1:2884157/148‑1 (MQ=255)
                                                                                     ccACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCttt                                                            <  1:314060/148‑1 (MQ=255)
                                                                                        cGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGa                                                         >  1:1978578/1‑148 (MQ=255)
                                                                                          atatTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGaaa                                                       >  1:2143730/1‑148 (MQ=255)
                                                                                           tatTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGaaaa                                                      <  1:3240759/148‑1 (MQ=255)
                                                                                            atTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGaaaaa                                                     >  1:429368/1‑148 (MQ=255)
                                                                                             ttCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGaaaaaa                                                    >  2:3474273/1‑147 (MQ=255)
                                                                                                aTTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTa                                                 <  2:2677580/148‑1 (MQ=255)
                                                                                                aTTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTa                                                 <  2:2677662/148‑1 (MQ=255)
                                                                                                  tttGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAAt                                               <  1:906800/148‑1 (MQ=255)
                                                                                                  tttGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAAt                                               >  1:3466472/1‑148 (MQ=255)
                                                                                                  tttGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAAt                                               >  1:1729183/1‑148 (MQ=255)
                                                                                                  tttGCTGGAAAAGTAAGACGATGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTGCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAAt                                               >  1:2437591/1‑148 (MQ=255)
                                                                                                     gCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCGAATTCg                                            >  2:798809/1‑148 (MQ=255)
                                                                                                      cTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGt                                           <  2:573579/148‑1 (MQ=255)
                                                                                                        ggAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAg                                         >  1:1043677/1‑148 (MQ=255)
                                                                                                        ggAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAg                                         >  2:2217699/1‑148 (MQ=255)
                                                                                                          aaaaGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGa                                       >  1:2963242/1‑148 (MQ=255)
                                                                                                          aaaaGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGa                                       <  1:976441/148‑1 (MQ=255)
                                                                                                           aaaGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGAt                                      <  2:2205945/148‑1 (MQ=255)
                                                                                                           aaaGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGAt                                      <  2:2944552/148‑1 (MQ=255)
                                                                                                            aaGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGAtt                                     >  2:2323198/1‑148 (MQ=255)
                                                                                                            aaGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGAtt                                     >  2:1113938/1‑148 (MQ=255)
                                                                                                             aGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTc                                    >  2:3397862/1‑148 (MQ=255)
                                                                                                                  gACGGTGCCATGCTGAGCGTCTTCAAAATAGTAAGAACGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTc                               <  2:2173382/148‑1 (MQ=255)
                                                                                                                   aCGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTcc                              <  2:1053567/148‑1 (MQ=255)
                                                                                                                   aCGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTcc                              <  1:3407887/148‑1 (MQ=255)
                                                                                                                     ggTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCa                            <  2:868152/148‑1 (MQ=255)
                                                                                                                     ggTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCa                            <  1:693002/148‑1 (MQ=255)
                                                                                                                       tGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAAc                                                    <  1:2350011/122‑1 (MQ=255)
                                                                                                                       tGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAAc                                                    >  2:2350011/1‑122 (MQ=255)
                                                                                                                       tGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACt                          >  2:429206/1‑148 (MQ=255)
                                                                                                                        gCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTg                         >  1:3319402/1‑148 (MQ=255)
                                                                                                                             gCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTAt                    >  1:2423469/1‑148 (MQ=255)
                                                                                                                               tGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTc                  >  1:1792702/1‑148 (MQ=255)
                                                                                                                               tGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTc                  >  2:2565507/1‑148 (MQ=255)
                                                                                                                               tGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTc                  >  1:3123667/1‑148 (MQ=255)
                                                                                                                                gAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTg                         >  1:2976835/1‑140 (MQ=255)
                                                                                                                                gAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTg                         <  2:2976835/140‑1 (MQ=255)
                                                                                                                                 aGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTcgc                >  1:1628023/1‑148 (MQ=255)
                                                                                                                                   cttcttCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCgg              >  1:2199453/1‑148 (MQ=255)
                                                                                                                                    ttcttcAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAACAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCgga             <  1:2221608/148‑1 (MQ=255)
                                                                                                                                     tcttcAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCggat            >  2:3292952/1‑148 (MQ=255)
                                                                                                                                       ttcAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCggatca          <  1:483606/148‑1 (MQ=255)
                                                                                                                                           aaaaaGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCggatcaggat      >  1:58274/1‑148 (MQ=255)
                                                                                                                                           aaaaaGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCggatcaggat      >  1:2329066/1‑148 (MQ=255)
                                                                                                                                           aaaaaGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCggatcaggat      >  2:669829/1‑148 (MQ=255)
                                                                                                                                            aaaaGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCggatcagtatc     >  2:320649/1‑148 (MQ=255)
                                                                                                                                               aGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGatcaggatcagg  >  1:2510913/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGG  >  CP009273/3519042‑3519332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGGTG  >  CP009273/3519042‑3519334
                                                                                                                                                  |                                                                                                                                                  
ATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGA                                                                                                                                                 <  SRR19973065.326409/150‑1 (MQ=60)
ATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGA                                                                                                                                                 <  SRR19973065.563371/150‑1 (MQ=60)
 TCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGAT                                                                                                                                                <  SRR19973065.2675307/150‑1 (MQ=60)
 TCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGAT                                                                                                                                                <  SRR19973065.2676565/150‑1 (MQ=60)
  CATCTGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATC                                                                                                                                               <  SRR19973065.1955274/150‑1 (MQ=60)
  CATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATC                                                                                                                                               >  SRR19973065.3241376/1‑150 (MQ=60)
  CATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATC                                                                                                                                               >  SRR19973065.2064428/1‑150 (MQ=60)
  CATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTACAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATC                                                                                                                                               <  SRR19973065.1262960/150‑1 (MQ=60)
   ATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCG                                                                                                                                              <  SRR19973065.2039944/150‑1 (MQ=60)
   ATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCG                                                                                                                                              <  SRR19973065.2039946/150‑1 (MQ=60)
     ATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTT                                                                                                                                            <  SRR19973065.3531444/150‑1 (MQ=60)
      TGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCAATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTC                                                                                                                                           <  SRR19973065.1465357/150‑1 (MQ=60)
      TGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCAATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTC                                                                                                                                           <  SRR19973065.1465422/150‑1 (MQ=60)
      TGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCAATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTC                                                                                                                                           <  SRR19973065.1465631/150‑1 (MQ=60)
       GCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCG                                                                                                                                          <  SRR19973065.348925/150‑1 (MQ=60)
          CCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTC                                                                                                                                       >  SRR19973065.1646224/1‑150 (MQ=60)
          CCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTC                                                                                                                                       >  SRR19973065.2959885/1‑150 (MQ=60)
          CCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTC                                                                                                                                       >  SRR19973065.2959884/1‑150 (MQ=60)
          CCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTC                                                                                                                                       >  SRR19973065.3394544/1‑150 (MQ=60)
          CCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTACAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTC                                                                                                                                       >  SRR19973065.2959617/1‑150 (MQ=60)
               GGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAAT                                                                                                                                  <  SRR19973065.3178053/150‑1 (MQ=60)
                       CACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTC                                                                                                                          >  SRR19973065.1076129/1‑150 (MQ=60)
                       CACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTC                                                                                                                          >  SRR19973065.2962445/1‑150 (MQ=60)
                       CACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTC                                                                                                                          >  SRR19973065.2220058/1‑150 (MQ=60)
                       CACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGAAAGATCGTTCGCTCCAAATCCAACCTC                                                                                                                          >  SRR19973065.2217067/1‑150 (MQ=60)
                           TGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCT                                                                                                                      <  SRR19973065.2471292/150‑1 (MQ=60)
                           TGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCT                                                                                                                      <  SRR19973065.1672394/150‑1 (MQ=60)
                               GTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTA                                                                                                                  <  SRR19973065.3090279/150‑1 (MQ=60)
                                TAGCGGCTCTGGCTCATCGCCTTCCTGTGATTGCGGGTTGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAG                                                                                                                 <  SRR19973065.2196818/150‑1 (MQ=60)
                                    GGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCG                                                                                                             <  SRR19973065.447621/150‑1 (MQ=60)
                                      CTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCG                                                                                                           <  SRR19973065.1593834/150‑1 (MQ=60)
                                         TGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTT                                                                                                        >  SRR19973065.2986031/1‑150 (MQ=60)
                                         TGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTT                                                                                                        <  SRR19973065.3499133/150‑1 (MQ=60)
                                         TGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTT                                                                                                        >  SRR19973065.2986039/1‑150 (MQ=60)
                                         TGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTT                                                                                                        <  SRR19973065.1603201/150‑1 (MQ=60)
                                         TGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTT                                                                                                        >  SRR19973065.2985881/1‑150 (MQ=60)
                                          GGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTA                                                                                                       >  SRR19973065.3354487/1‑150 (MQ=60)
                                             TCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCG                                                                                                    <  SRR19973065.2793364/150‑1 (MQ=60)
                                               gagttcagacgtgtgctcttccgatctATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGC                                                                                                  <  SRR19973065.155482/123‑1 (MQ=60)
                                                 CGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTT                                                                                                <  SRR19973065.2247572/150‑1 (MQ=60)
                                                 CGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCGT                                                                                                >  SRR19973065.3117072/1‑150 (MQ=60)
                                                  GCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTC                                                                                               <  SRR19973065.400605/150‑1 (MQ=60)
                                                   CCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCG                                                                                              <  SRR19973065.1872296/150‑1 (MQ=60)
                                                    CTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGT                                                                                             <  SRR19973065.431477/150‑1 (MQ=60)
                                                         AGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACG                                                                                        <  SRR19973065.3338060/150‑1 (MQ=60)
                                                            GATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTT                                                                                     <  SRR19973065.1130103/150‑1 (MQ=60)
                                                            GATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTT                                                                                     <  SRR19973065.1129745/150‑1 (MQ=60)
                                                              TTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTC                                                                                   <  SRR19973065.1321441/150‑1 (MQ=60)
                                                               TCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCA                                                                                  <  SRR19973065.3103485/150‑1 (MQ=60)
                                                                  GGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCC                                                                               >  SRR19973065.1873160/1‑150 (MQ=60)
                                                                       GAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATC                                                                          <  SRR19973065.3187084/150‑1 (MQ=60)
                                                                       GAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATC                                                                          <  SRR19973065.3187052/150‑1 (MQ=60)
                                                                       GAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATC                                                                          <  SRR19973065.2479942/150‑1 (MQ=60)
                                                                       GAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATC                                                                          <  SRR19973065.1177717/150‑1 (MQ=60)
                                                                           TCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATT                                                                      <  SRR19973065.513015/150‑1 (MQ=60)
                                                                              TGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAAT                                                                   >  SRR19973065.1053107/1‑150 (MQ=60)
                                                                               GCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATC                                                                  <  SRR19973065.1121226/150‑1 (MQ=60)
                                                                                CGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCC                                                                 <  SRR19973065.1834140/150‑1 (MQ=60)
                                                                                   TACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTT                                                              >  SRR19973065.1130384/1‑150 (MQ=60)
                                                                                   TACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTT                                                              >  SRR19973065.283015/1‑150 (MQ=60)
                                                                                    ACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTC                                                             <  SRR19973065.1223181/150‑1 (MQ=60)
                                                                                    ACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTC                                                             <  SRR19973065.2965898/150‑1 (MQ=60)
                                                                                    ACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTC                                                             <  SRR19973065.1759436/150‑1 (MQ=60)
                                                                                     CCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCG                                                            <  SRR19973065.316407/150‑1 (MQ=60)
                                                                                     CCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCG                                                            <  SRR19973065.316185/150‑1 (MQ=60)
                                                                                     CCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCG                                                            <  SRR19973065.2901800/150‑1 (MQ=60)
                                                                                      CACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGA                                                           >  SRR19973065.1991447/1‑150 (MQ=60)
                                                                                        CGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAA                                                         >  SRR19973065.3175603/1‑150 (MQ=60)
                                                                                        CGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAA                                                         >  SRR19973065.2157519/1‑150 (MQ=60)
                                                                                        CGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAA                                                         >  SRR19973065.2157538/1‑150 (MQ=60)
                                                                                         GATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAA                                                        >  SRR19973065.1477888/1‑150 (MQ=60)
                                                                                          ATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAA                                                       >  SRR19973065.432349/1‑150 (MQ=60)
                                                                                           TATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAAC                                                      <  SRR19973065.3260288/150‑1 (MQ=60)
                                                                                            ATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACC                                                     >  SRR19973065.754371/1‑150 (MQ=60)
                                                                                             ttcagacgtgtgctcttccgatctGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCT                                                    <  SRR19973065.1923144/126‑1 (MQ=60)
                                                                                             ttcagacgtgtgctcttccgatctGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCT                                                    <  SRR19973065.2364649/126‑1 (MQ=60)
                                                                                                ATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAAT                                                 >  SRR19973065.1740641/1‑150 (MQ=60)
                                                                                                ATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAAT                                                 >  SRR19973065.2452961/1‑150 (MQ=60)
                                                                                                ATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAAT                                                 >  SRR19973065.680038/1‑150 (MQ=60)
                                                                                                ATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAAT                                                 >  SRR19973065.3487583/1‑150 (MQ=60)
                                                                                                ATTTTGCTGGAAAAGTAAGACGATGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTGCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAAT                                                 >  SRR19973065.2452962/1‑150 (MQ=60)
                                                                                                  TTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTC                                               <  SRR19973065.2309592/150‑1 (MQ=60)
                                                                                                  TTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTC                                               <  SRR19973065.912917/150‑1 (MQ=60)
                                                                                                  TTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTC                                               <  SRR19973065.912673/150‑1 (MQ=60)
                                                                                                  TTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTC                                               <  SRR19973065.582612/150‑1 (MQ=60)
                                                                                                      CTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAG                                           >  SRR19973065.1050576/1‑150 (MQ=60)
                                                                                                        GGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGA                                         >  SRR19973065.2981344/1‑150 (MQ=60)
                                                                                                          AAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATT                                       <  SRR19973065.982926/150‑1 (MQ=60)
                                                                                                          AAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATT                                       <  SRR19973065.2088227/150‑1 (MQ=60)
                                                                                                              GTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGT                                   <  SRR19973065.704862/150‑1 (MQ=60)
                                                                                                                   ACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCA                              <  SRR19973065.1680862/150‑1 (MQ=60)
                                                                                                                   ACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCA                              <  SRR19973065.1970773/150‑1 (MQ=60)
                                                                                                                   ACGGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCA                              <  SRR19973065.3428708/150‑1 (MQ=60)
                                                                                                                     GGTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACT                            <  SRR19973065.697616/150‑1 (MQ=60)
                                                                                                                      GTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTG                           >  SRR19973065.3339610/1‑150 (MQ=60)
                                                                                                                      GTGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTG                           >  SRR19973065.3339652/1‑150 (MQ=60)
                                                                                                                       TGCCATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGC                          >  SRR19973065.241366/1‑150 (MQ=60)
                                                                                                                           ATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTAT                      >  SRR19973065.2403882/1‑150 (MQ=60)
                                                                                                                           ATGCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTAT                      >  SRR19973065.2438752/1‑150 (MQ=60)
                                                                                                                           AGGCTGCGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAAACCCACCTCTTCATTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATGAATCCTTTCGAAAAACCGAATTCGTAGGATTCAGTTCCCACTGCGTAT                      <  SRR19973065.2363242/150‑1 (MQ=60)
                                                                                                                             GCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTC                    >  SRR19973065.3142691/1‑150 (MQ=60)
                                                                                                                             GCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTC                    >  SRR19973065.1804462/1‑150 (MQ=60)
                                                                                                                             GCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTC                    >  SRR19973065.3143059/1‑150 (MQ=60)
                                                                                                                             GCTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTC                    >  SRR19973065.3142690/1‑150 (MQ=60)
                                                                                                                              CTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGagatcg                   >  SRR19973065.2995047/1‑144 (MQ=60)
                                                                                                                              CTGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAAACTAATTCGTATGATTCAGTTCCCACTGCGagatcg                   >  SRR19973065.2995048/1‑144 (MQ=60)
                                                                                                                               TNAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGC                  >  SRR19973065.1638935/1‑150 (MQ=60)
                                                                                                                               TGAGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGC                  >  SRR19973065.1638998/1‑150 (MQ=60)
                                                                                                                                 AGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGG                >  SRR19973065.2213534/1‑150 (MQ=60)
                                                                                                                                 AGCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTATCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGG                <  SRR19973065.1360694/150‑1 (MQ=60)
                                                                                                                                  GCTTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGA               <  SRR19973065.2455949/150‑1 (MQ=60)
                                                                                                                                    TTCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAACAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATC             <  SRR19973065.2235783/150‑1 (MQ=60)
                                                                                                                                     TCTTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCA            >  SRR19973065.2946242/1‑150 (MQ=60)
                                                                                                                                       TTCAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGG          <  SRR19973065.486894/150‑1 (MQ=60)
                                                                                                                                         CAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGAT        >  SRR19973065.3418418/1‑150 (MQ=60)
                                                                                                                                         CAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGAT        >  SRR19973065.58895/1‑150 (MQ=60)
                                                                                                                                         CAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGAT        >  SRR19973065.2343636/1‑150 (MQ=60)
                                                                                                                                         CAAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGAT        >  SRR19973065.3418358/1‑150 (MQ=60)
                                                                                                                                          AAAAAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATC       <  SRR19973065.2229069/150‑1 (MQ=60)
                                                                                                                                             AAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGG    >  SRR19973065.2526692/1‑150 (MQ=60)
                                                                                                                                             AAAGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGG    >  SRR19973065.2524725/1‑150 (MQ=60)
                                                                                                                                               AGTAAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGGTG  >  SRR19973065.1475307/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                  
ATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAGGTG  >  CP009273/3519042‑3519334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑