Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,066,133 T→G 100% S267R (AGC→CGC)  yihU ← gamma‑hydroxybutyrate dehydrogenase, NADH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,066,1330TG99.5% 710.4 / ‑5.3 180S267R (AGC→CGC) yihUgamma‑hydroxybutyrate dehydrogenase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (97/81);  total (98/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 181 total aligned reads displayed.

GTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGA  >  CP009273/4065988‑4066277
                                                                                                                                                 |                                                                                                                                                
gTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCgat                                                                                                                                                <  2:3113943/148‑1 (MQ=255)
     tGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTAAAATTTTAATTTTGCAGTCATACCCGCACAGACACCGACCTGTTACACAATTGCGGACCAATACAGGCGACCGCCACCCCCTGCGCGCCCCAGGCGATaaaac                                                                                                                                           >  1:1946560/1‑146 (MQ=255)
       aTGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTc                                                                                                                                         <  1:937897/148‑1 (MQ=255)
       aTGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTAATTTGGCAGTAATCCCGGCACTGACACGGACCTGTTACAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAAACTc                                                                                                                                         >  2:3037596/1‑148 (MQ=255)
            gtACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTg                                                                                                                                    <  1:167128/148‑1 (MQ=255)
            gtACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTAATTTTTCAGTCATACCGGCACTGACACCGACCAGTTACAGAATGGCGGCCCACTCCTGGCGACCCCCACCCCCCGCGCGCGCCGGGCGCTAAACCACCCCTg                                                                                                                                    >  1:2089914/1‑148 (MQ=255)
            gtACTTATTCATTCATTTATTCCTTTATCCAGTCGTGACATTTTTACTTTTGCAGTCATACCCGCACAGACACCGACCAGTTCCACAATTGCGGACCAGTACAGGCGACCCCCACCCCCAGCGCGCGCCAGGCGATAAACCAccccag                                                                                                                                    >  1:1794365/1‑145 (MQ=255)
             tACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTTGCAGTCATCCCGGCCCTGACACGGACCTGTTCCCCAATGGCGGACCAGTCCAGGGGACCCCGACCCGCTGCGCGCCCCAGGCGCTAAACCTACCCTGa                                                                                                                                   >  2:372367/1‑148 (MQ=255)
             tACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGa                                                                                                                                   <  2:7251/148‑1 (MQ=255)
               cTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAgg                                                                                                                                 <  1:2090946/148‑1 (MQ=255)
               cTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGGTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAgg                                                                                                                                 <  2:1352251/148‑1 (MQ=255)
                 tATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTTGCAGTCAAACCCGCACAGACACGGACCAGTTACAGAAATGCGGACCAGTCCAGGCGACCCCCACCCCCAGCGCGCGCCTGGCGATAAAACAACCCTGAggcg                                                                                                                               >  1:1644152/1‑148 (MQ=255)
                  attcattcATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAggcgg                                                                                                                              <  2:743659/148‑1 (MQ=255)
                    tcattcatTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTGCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGcc                                                                                                                            >  2:1887875/1‑148 (MQ=255)
                     cattcattTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGccc                                                                                                                           <  2:1930006/148‑1 (MQ=255)
                     cattcattTATTCCTTTATCCAGTCGTGTCATTTTTAATTTTGCAGTCAAACCCGCACATAAACCGACCAGTTACAGAAAGGCGGACCACTCCTGGCGACCCCCACCCCCAGCCCCCCCCAGGCGATAAAACACCCCGGAGGCGGccc                                                                                                                           >  1:2326450/1‑148 (MQ=255)
                      attcattTATTCCTTTATGCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGGCACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGcccc                                                                                                                          <  2:1029943/148‑1 (MQ=255)
                      attcattTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGcccc                                                                                                                          <  2:2298899/148‑1 (MQ=255)
                       ttcattTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTACAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCACCCGTGAGGCGGccccc                                                                                                                         >  2:2647578/1‑148 (MQ=255)
                       ttcattTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTAAACCCCGCACTGACACGGACCTGTTCCAGAATTGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGCTAAACCTCCCGTGAGGCGGccccc                                                                                                                         >  2:2924631/1‑148 (MQ=255)
                       ttcattTATTCCTTTATCCAGTCGTTACAATTTTACTTTGGCAGTCATACCGGCACAGACAAGGACCTGTTCCAGAATGGCGGACCAGTCCTCGCGACCCCGAACCGCTGCGCGCGCCAGGCGATAAAACTCCCCTGATGCGGccccc                                                                                                                         >  2:2925608/1‑148 (MQ=255)
                        tcattTATTCCTTTATCCAGTCGTTACAATTTTAATTTGGCAGTCAACCCCGCACGGACACCGACCAGTTACAGAATGGCGGACCAGTCCTGGCGACCCCGACCCCCTGCGCGCGCCAGGCGATAAACCACCCGTGAGGCGGCCCCCa                                                                                                                        >  1:1103504/1‑148 (MQ=255)
                        tcattTATTCCTTTATCCAGTCGTGTCAATTTTAATTTTGGAGTCAACCCCGCACAGACACGGACCTGTTCCAGAATGGCGGACCACTCCAGGCGCCCCCGACCCCCTGCGCGCGCCGGGCGATAAAACACCCCGGAGGCGGcccccc                                                                                                                        >  1:466879/1‑147 (MQ=255)
                          attTATTCCTTTATCCAGTCGTTACATTTTTAATTTTGCAGTCAACCCGGCACAGACACCGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCCCCACCCCCGGCGCGCGCCAGGCGCGAAAACACCCCTGAGGCGGcccccccc                                                                                                                      >  2:945152/1‑145 (MQ=255)
                            ttATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCgg                                                                                                                    <  1:2527501/148‑1 (MQ=255)
                               ttCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCAGTGAGGCGGCCCCCAGCGGCAt                                                                                                                 <  1:2806929/148‑1 (MQ=255)
                                  cTTTATCCAGTCGTTACAATTTTAATTTTGCAGTAATACCCGCACTGAAAAGGACCAGTTCAAGAATGGCGGAACAGTCCTGGCGACCGCGACCCGCAGCGCGCGCCTGGCGAAAAAACACCCCTGAGGCGGCCCCCAGCCGCAtttg                                                                                                              >  2:1522173/1‑146 (MQ=255)
                                        ccAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATg                                                                                                        <  1:2206414/148‑1 (MQ=255)
                                        ccAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATg                                                                                                        <  2:1179073/148‑1 (MQ=255)
                                         cAGTCGTGACAATTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGc                                                                                                       <  2:2201658/148‑1 (MQ=255)
                                            tCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGc                                                                                                    >  2:2820634/1‑148 (MQ=255)
                                               ttACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTgg                                                                                                 >  2:1274942/1‑148 (MQ=255)
                                                tACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGt                                                                                                >  1:51258/1‑148 (MQ=255)
                                                tACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGt                                                                                                >  1:1171820/1‑148 (MQ=255)
                                                tACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGt                                                                                                >  1:51257/1‑148 (MQ=255)
                                                 aCATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGtt                                                                                               <  1:1996062/148‑1 (MQ=255)
                                                  cATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTg                                                                                              <  2:3051063/148‑1 (MQ=255)
                                                  cATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTg                                                                                              >  2:71429/1‑148 (MQ=255)
                                                  cATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTg                                                                                              >  2:70818/1‑148 (MQ=255)
                                                       ttACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGAc                                                                                         <  2:1459270/148‑1 (MQ=255)
                                                       ttACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGAc                                                                                         <  2:1035743/148‑1 (MQ=255)
                                                        tACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACa                                                                                        >  1:1629860/1‑148 (MQ=255)
                                                          cTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATc                                                                                      >  1:685041/1‑148 (MQ=255)
                                                               gCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAggg                                                                                 >  2:2679980/1‑148 (MQ=255)
                                                               gCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAggg                                                                                 <  1:1301012/148‑1 (MQ=255)
                                                                cAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGc                                                                                >  1:353345/1‑148 (MQ=255)
                                                                 aGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCg                                                                               <  1:1061182/148‑1 (MQ=255)
                                                                  gTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGa                                                                              >  2:1956004/1‑148 (MQ=255)
                                                                    cATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATg                                                                            <  1:1658238/148‑1 (MQ=255)
                                                                    cATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATg                                                                            >  1:644370/1‑148 (MQ=255)
                                                                     aTCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGc                                                                           <  2:2380187/148‑1 (MQ=255)
                                                                      tCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCATCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGcc                                                                          >  1:1311135/1‑148 (MQ=255)
                                                                       cccGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCa                                                                         >  2:2060181/1‑148 (MQ=255)
                                                                            cACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAATATc                                                                    <  2:3157944/148‑1 (MQ=255)
                                                                              cTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCt                                                                  >  1:2038383/1‑148 (MQ=255)
                                                                               tGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCtt                                                                 >  2:3008633/1‑148 (MQ=255)
                                                                                 acacGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTAt                                                               >  1:2307417/1‑148 (MQ=255)
                                                                                 acacGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTAt                                                               >  2:3123337/1‑148 (MQ=255)
                                                                                      gACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCa                                                          >  1:3122332/1‑148 (MQ=255)
                                                                                       aCCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCaa                                                         >  1:1631689/1‑148 (MQ=255)
                                                                                           gTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatc                                                     >  2:2056130/1‑148 (MQ=255)
                                                                                              ccAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatcgat                                                  >  2:1005078/1‑148 (MQ=255)
                                                                                                  aaTGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATg                                              <  1:2432829/148‑1 (MQ=255)
                                                                                                  aaTGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATg                                              >  1:793379/1‑148 (MQ=255)
                                                                                                  aaTGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATg                                              <  2:2091875/148‑1 (MQ=255)
                                                                                                    tGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGaa                                            >  1:1438983/1‑148 (MQ=255)
                                                                                                    tGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGaa                                            >  2:2907184/1‑148 (MQ=255)
                                                                                                    tGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGaa                                            <  1:3027979/148‑1 (MQ=255)
                                                                                                    tGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGaa                                            <  1:3027981/148‑1 (MQ=255)
                                                                                                     ggCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAg                                           <  2:1654237/148‑1 (MQ=255)
                                                                                                      gCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAgg                                          >  2:1183819/1‑148 (MQ=255)
                                                                                                       cGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGc                                         <  1:1336388/148‑1 (MQ=255)
                                                                                                        ggACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCg                                        <  1:2486292/148‑1 (MQ=255)
                                                                                                         gACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCgg                                       >  1:2391224/1‑148 (MQ=255)
                                                                                                         gACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTAGGGGCAAGATCGATCATGAAGGCgg                                       >  1:2390786/1‑148 (MQ=255)
                                                                                                          aCCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCggg                                      <  1:2123128/148‑1 (MQ=255)
                                                                                                           ccAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGga                                     <  1:109928/148‑1 (MQ=255)
                                                                                                            cAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGgag                                    >  1:1206432/1‑148 (MQ=255)
                                                                                                              gTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGaa                                  <  1:1852068/148‑1 (MQ=255)
                                                                                                                ccTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAg                                <  2:2143969/148‑1 (MQ=255)
                                                                                                                 cTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGa                               <  1:1545130/148‑1 (MQ=255)
                                                                                                                  tGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAt                              >  1:2966714/1‑148 (MQ=255)
                                                                                                                    gcgaccgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATCATGAAGGCGGGAGAAAGAtct                            >  2:1622146/1‑148 (MQ=255)
                                                                                                                    gcgaccgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAtct                            >  1:3218589/1‑148 (MQ=255)
                                                                                                                    gcgaccgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAtct                            >  2:1622162/1‑148 (MQ=255)
                                                                                                                    gcgaccgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAtct                            <  2:1974755/148‑1 (MQ=255)
                                                                                                                     cgaccgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAtctc                           <  2:2669356/148‑1 (MQ=255)
                                                                                                                       accgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCg                         >  2:1396410/1‑148 (MQ=255)
                                                                                                                       accgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCg                         <  1:2657560/148‑1 (MQ=255)
                                                                                                                       accgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCg                         >  1:581983/1‑148 (MQ=255)
                                                                                                                        ccgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGc                        <  1:1628416/148‑1 (MQ=255)
                                                                                                                           cgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGa                     >  2:598546/1‑148 (MQ=255)
                                                                                                                              cccGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGa                  <  1:2134884/148‑1 (MQ=255)
                                                                                                                                       cgcgcCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACtttgtttg         >  2:1149247/1‑148 (MQ=255)
                                                                                                                                        gcgcCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTgg        <  2:1255096/148‑1 (MQ=255)
                                                                                                                                         cgcCTGGCGATGAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGc       <  2:760055/148‑1 (MQ=255)
                                                                                                                                         cgcCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGc       <  2:400124/148‑1 (MQ=255)
                                                                                                                                           ccTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGAGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTTGCCa     <  1:71429/148‑1 (MQ=255)
                                                                                                                                              ggCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGa  >  2:1011595/1‑148 (MQ=255)
                                                                                                                                              ggCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGACGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGa  >  2:3055460/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGA  >  CP009273/4065988‑4066277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGG  >  CP009273/4065984‑4066282
                                                                                                                                                     |                                                                                                                                                     
TAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCG                                                                                                                                                       <  SRR19973070.706918/150‑1 (MQ=60)
           ATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCC                                                                                                                                            <  SRR19973070.944579/150‑1 (MQ=60)
              GTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTTCATTTTTAATTTGGGAGTCAAACCCGCACAGACACCGACCAGTTCCAGAAAGGCGGACCACTCCAGGCGACCCCGACCCCCTGCGCGCGCCAGGCGATAAACCaccccgg                                                                                                                                         >  SRR19973070.123269/1‑143 (MQ=60)
              GTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTGACATTTTTACTTTTGCAGTCATACCCGCACAGACACCGACCAGTTCCACAATTGCGGACCAGTACAGGCGACCCCCACCCCCAGCGCGCGCCAGGCGATAAACCaccccag                                                                                                                                         >  SRR19973070.1806759/1‑143 (MQ=52)
                GTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAG                                                                                                                                       <  SRR19973070.168491/150‑1 (MQ=60)
                   CTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTTGCAGTCAAACCCGCACAGACACGGACCAGTTACAGAAATGCGGACCAGTCCAGGCGACCCCCACCCCCAGCGCGCGCCTGGCGATAAAacaaccctgaggcg                                                                                                                                    >  SRR19973070.1655513/1‑136 (MQ=59)
                   CTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCG                                                                                                                                    <  SRR19973070.2105003/150‑1 (MQ=60)
                          ATTCATTTATTCCTTTATCCAGTCGTTACATTTTTAATTTTGCAGTCAAACCCGCCCGGACACGGACCAGTTGCAGAAAGGCGGACCACTCCAGGCGAACGCGACCCCCAGCGCGCGCCAGGCGATAAACCACCCCTGAGGCGGCCCCCC                                                                                                                             >  SRR19973070.470296/1‑150 (MQ=44)
                          ATTCATTTATTCCTTTATCCAGTCGTTACAATTTTAATTTTGCAGTCAACCCCGCACAGACACGGACCAGTTACAGAAAGGCGGACCAGTCCAGGCGACCCCCACCCGCTGCGCGCGCCTGGCGATAAAACAACCGTGAGGCGGCCCCCA                                                                                                                             >  SRR19973070.1110351/1‑150 (MQ=60)
                          ATTCATTTATTCCTTTATCCAGTCGTTACAATTTTAATTTGGCAGTCAACCCCGCACGGACACCGACCAGTTACAGAATGGCGGACCAGTCCTGGCGACCCCGACCCCCTGCGCGCGCCAGGCGATAAACCACCCGTGAGGCGGCCCCCA                                                                                                                             >  SRR19973070.1111077/1‑150 (MQ=60)
                          ATTCATTTATTCCTTTATCCAGTCGTGTCAATTTTAATTTTGGAGTCAACCCCGCACAGACACGGACCTGTTCCAGAATGGCGGACCACTCCAGGCGCCCCCGACCCCCTGCGCGCGCCGGGCGATAAAACACCCCGGAGGCGGCCCCCC                                                                                                                             >  SRR19973070.470200/1‑150 (MQ=36)
                             CATTTATTCCTTTATCCAGTCGTTACATTTTTAATTTTGCAGTCATCCCCGCACGGACACCGACCAGTTACAGAAAGGCGGACCAGTCCAGGCGACCGCCACCCCCTGCGCGCGCCGGGCGATAAAACACCCCGGAGGCGGCCCCCCGCG                                                                                                                          >  SRR19973070.1665641/1‑150 (MQ=55)
                                TTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCA                                                                                                                       <  SRR19973070.2544003/150‑1 (MQ=60)
                                   TTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCAGTGAGGCGGCCCCCAGCGGCATTG                                                                                                                    <  SRR19973070.2825070/150‑1 (MQ=60)
                                    TCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGG                                                                                                                   <  SRR19973070.608145/150‑1 (MQ=60)
                                            CCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCA                                                                                                           <  SRR19973070.2220938/150‑1 (MQ=60)
                                             CAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAG                                                                                                          <  SRR19973070.1852110/150‑1 (MQ=60)
                                                 CGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGG                                                                                                      >  SRR19973070.452063/1‑150 (MQ=60)
                                                  GTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGT                                                                                                     >  SRR19973070.51881/1‑150 (MQ=60)
                                                  GTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGT                                                                                                     >  SRR19973070.51880/1‑150 (MQ=60)
                                                  GTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGT                                                                                                     >  SRR19973070.1180028/1‑150 (MQ=60)
                                                  GTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGT                                                                                                     >  SRR19973070.1180092/1‑150 (MQ=60)
                                                     ACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGG                                                                                                  <  SRR19973070.2009756/150‑1 (MQ=60)
                                                     ACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGG                                                                                                  <  SRR19973070.2009590/150‑1 (MQ=60)
                                                     ACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGG                                                                                                  <  SRR19973070.2009477/150‑1 (MQ=60)
                                                          TTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACA                                                                                             >  SRR19973070.1641171/1‑150 (MQ=60)
                                                          TTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACA                                                                                             >  SRR19973070.1641191/1‑150 (MQ=60)
                                                           TTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACAT                                                                                            <  SRR19973070.525967/150‑1 (MQ=60)
                                                            TACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATC                                                                                           >  SRR19973070.689926/1‑150 (MQ=60)
                                                                  GGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGC                                                                                     >  SRR19973070.355921/1‑150 (MQ=60)
                                                                   GCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCG                                                                                    <  SRR19973070.1310058/150‑1 (MQ=60)
                                                                    CAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGA                                                                                   >  SRR19973070.1348607/1‑150 (MQ=60)
                                                                     AGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGAT                                                                                  <  SRR19973070.1068624/150‑1 (MQ=60)
                                                                      GTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATG                                                                                 >  SRR19973070.648969/1‑150 (MQ=60)
                                                                        CATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCATCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCC                                                                               >  SRR19973070.1320231/1‑150 (MQ=60)
                                                                        CATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCC                                                                               <  SRR19973070.1669661/150‑1 (MQ=60)
                                                                        CATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCC                                                                               <  SRR19973070.3165038/150‑1 (MQ=60)
                                                                                CACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCT                                                                       >  SRR19973070.2052177/1‑150 (MQ=60)
                                                                                CACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCT                                                                       >  SRR19973070.2052196/1‑150 (MQ=60)
                                                                                   TGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTAT                                                                    >  SRR19973070.650249/1‑150 (MQ=60)
                                                                                   TGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTAT                                                                    <  SRR19973070.2704437/150‑1 (MQ=60)
                                                                                   TGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTAT                                                                    >  SRR19973070.2322756/1‑150 (MQ=60)
                                                                                     ACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGG                                                                  <  SRR19973070.1551803/150‑1 (MQ=60)
                                                                                        CGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCA                                                               >  SRR19973070.3142488/1‑150 (MQ=60)
                                                                                         GGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAA                                                              >  SRR19973070.1643006/1‑150 (MQ=60)
                                                                                           ACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGA                                                            <  SRR19973070.2218783/150‑1 (MQ=60)
                                                                                              TGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCG                                                         <  SRR19973070.526965/150‑1 (MQ=60)
                                                                                               GTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGA                                                        >  SRR19973070.2135391/1‑150 (MQ=60)
                                                                                                  CCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCA                                                     <  SRR19973070.1681060/150‑1 (MQ=60)
                                                                                                    AGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATG                                                   >  SRR19973070.798868/1‑150 (MQ=60)
                                                                                                     GAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGA                                                  >  SRR19973070.2444170/1‑150 (MQ=60)
                                                                                                      AATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAA                                                 <  SRR19973070.2448792/150‑1 (MQ=60)
                                                                                                      AATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAA                                                 >  SRR19973070.1448957/1‑150 (MQ=60)
                                                                                                        TGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGG                                               <  SRR19973070.3047260/150‑1 (MQ=60)
                                                                                                        TGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGG                                               <  SRR19973070.3047258/150‑1 (MQ=60)
                                                                                                           CGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCCCGATCGATCATGAAGGCGG                                            >  SRR19973070.2406537/1‑150 (MQ=60)
                                                                                                           CGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGG                                            >  SRR19973070.2406930/1‑150 (MQ=60)
                                                                                                           CGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGG                                            >  SRR19973070.2407011/1‑150 (MQ=60)
                                                                                                           CGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGG                                            <  SRR19973070.1345585/150‑1 (MQ=60)
                                                                                                           CGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTAGGGGCAAGATCGATCATGAAGGCGG                                            >  SRR19973070.2406572/1‑150 (MQ=60)
                                                                                                            GGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGgaagagcacacgtc                                           >  SRR19973070.3073341/1‑136 (MQ=60)
                                                                                                            GGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGG                                           <  SRR19973070.832992/150‑1 (MQ=60)
                                                                                                            GGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGG                                           <  SRR19973070.2502449/150‑1 (MQ=60)
                                                                                                             GACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGA                                          <  SRR19973070.951881/150‑1 (MQ=60)
                                                                                                              ACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAG                                         >  SRR19973070.1214905/1‑150 (MQ=60)
                                                                                                              ACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAG                                         <  SRR19973070.2137338/150‑1 (MQ=60)
                                                                                                              ACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAG                                         <  SRR19973070.2137348/150‑1 (MQ=60)
                                                                                                               CCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGA                                        <  SRR19973070.110947/150‑1 (MQ=60)
                                                                                                                  GTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATCATGAAGGCGGGAGAAAG                                     >  SRR19973070.3334023/1‑150 (MQ=60)
                                                                                                                  GTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAG                                     <  SRR19973070.1864758/150‑1 (MQ=60)
                                                                                                                  GTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAG                                     <  SRR19973070.1864910/150‑1 (MQ=60)
                                                                                                                    CCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCCAGATCGATCATGAAGGCGGGAGAAAGAT                                   >  SRR19973070.2984939/1‑150 (MQ=60)
                                                                                                                    CCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAT                                   >  SRR19973070.2985780/1‑150 (MQ=60)
                                                                                                                     CTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATC                                  >  SRR19973070.1342689/1‑150 (MQ=60)
                                                                                                                     CTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATC                                  <  SRR19973070.1555992/150‑1 (MQ=60)
                                                                                                                      TGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCT                                 >  SRR19973070.3239394/1‑150 (MQ=60)
                                                                                                                         CGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCCAGATCGATCATGAAGGCGGGAGAAAGATCTCCG                              >  SRR19973070.2258780/1‑150 (MQ=60)
                                                                                                                         CGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCG                              >  SRR19973070.585992/1‑150 (MQ=60)
                                                                                                                           ACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCT                            <  SRR19973070.2674846/150‑1 (MQ=60)
                                                                                                                            CCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTG                           <  SRR19973070.1639717/150‑1 (MQ=60)
                                                                                                                                  CCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACT                     <  SRR19973070.2149144/150‑1 (MQ=60)
                                                                                                                                  CCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACT                     <  SRR19973070.2493/150‑1 (MQ=60)
                                                                                                                                       TGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTT                <  SRR19973070.3015506/150‑1 (MQ=60)
                                                                                                                                            GCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCC           <  SRR19973070.1616164/150‑1 (MQ=60)
                                                                                                                                               CCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGG        <  SRR19973070.2635181/150‑1 (MQ=60)
                                                                                                                                               CCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGG        <  SRR19973070.71589/150‑1 (MQ=60)
                                                                                                                                               CCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCGGGTTGGCGACATCAAGGGAGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTTGCCAGG        <  SRR19973070.72202/150‑1 (MQ=60)
                                                                                                                                                    CGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCCCGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTG   >  SRR19973070.1839212/1‑150 (MQ=60)
                                                                                                                                                    CGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTG   <  SRR19973070.2024739/150‑1 (MQ=60)
                                                                                                                                                    CGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTG   <  SRR19973070.1361043/150‑1 (MQ=60)
                                                                                                                                                    CGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTG   <  SRR19973070.2838871/150‑1 (MQ=60)
                                                                                                                                                     GATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACNTGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGNTCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGG  >  SRR19973070.2135122/1‑150 (MQ=60)
                                                                                                                                                     GATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGG  >  SRR19973070.505610/1‑150 (MQ=60)
                                                                                                                                                     GATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGG  >  SRR19973070.1606485/1‑150 (MQ=60)
                                                                                                                                                     GATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGG  >  SRR19973070.1385055/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGG  >  CP009273/4065984‑4066282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑