Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,066,133 T→G 100% S267R (AGC→CGC)  yihU ← gamma‑hydroxybutyrate dehydrogenase, NADH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,066,1330TG100.0% 686.3 / ‑6.7 175S267R (AGC→CGC) yihUgamma‑hydroxybutyrate dehydrogenase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base G (85/87);  minor base C (2/0);  total (88/87)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 177 total aligned reads displayed.

TCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCG‑TTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGA  >  CP009273/4065989‑4066277
                                                                                                                                                 |                                                                                                                                                
   ttGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCG‑TTACATTTTTACTTTGGCAGTCATCCCGGCCCTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAAc                                                                                                                                            >  2:2448093/1‑148 (MQ=255)
   ttGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCG‑TTACATTTTTACTTTGGCAGTCATCACGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAAc                                                                                                                                            >  2:2448046/1‑148 (MQ=255)
   ttGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCG‑TTACATTTTTACGTTTGCAGGGATCCCGGCACTGACAAGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCAGGCGATAAAc                                                                                                                                            >  2:2733650/1‑148 (MQ=255)
       tGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCG‑TTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTcc                                                                                                                                        <  1:867550/148‑1 (MQ=255)
       tGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCG‑TTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTcc                                                                                                                                        <  2:196167/148‑1 (MQ=255)
       tGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCG‑TTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTcc                                                                                                                                        <  1:202949/148‑1 (MQ=255)
        ggTGTACTTATTCATTCATTTATTCCTTTATCCAGTCG‑TTACATTTTTACTTTTGCACTCAAACCGGCACTGACAAGGACCTGTTACAGAAAGGCCGACCAGTCCTGGCGACCGCGACCCGCAGCGCGCGCCTGGCGATAAACCTccc                                                                                                                                       >  2:2332735/1‑148 (MQ=255)
           gtACTTATTCATTCATTTATTCCTTTATCCAGTCG‑TTACATTTTTAATTTTGCAGTCATACCGGCACTGACACGGACCTGTTACAGAATTGCGGACCCGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTg                                                                                                                                    >  2:1743870/1‑148 (MQ=255)
           gtACTTATTCATTCATTTATTCCTTTATCCAGTCG‑TTACAATTTTAATTTTGCAGTCATACCCGCACAGAAACGGACCGGTTCAAGAATTGCGGACCACTCCTGGCGACCCCGACCCCCTGCGCCCGCCTGGCGCTAAAACACCCCTg                                                                                                                                    >  1:3741375/1‑148 (MQ=255)
              cTTATTCATTCATTTATTCCTTTATCCAGTCG‑TTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAgg                                                                                                                                 <  1:608005/148‑1 (MQ=255)
              cTTATTCATTCATTTATTCCTTTATCCAGTCG‑TTAAATTTTTACTTTGGCAGTCAACCCGGCACTGACACCGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTTGCTATAAACCTACCCTGAgg                                                                                                                                 >  2:2177972/1‑148 (MQ=255)
                tATTCATTCATTTATTCCTTTATCCAGTCG‑TTACATTTTTAATTTTGCAGTCATCCCGGCACTGACACGGACCAGTTCCAGAATGGCGGACCAGTCCAGGCGACCCCGACCCGCGGCGCGCGCCAGGCGATAAACCACCCCTGAggcg                                                                                                                               >  2:816505/1‑148 (MQ=255)
                tATTCATTCATTTATTCCTTTATCCAGTCG‑TTACAATTTTACTTTTGCAGTCAACCCGGCACTGAAACCGACCAGTTACAGAATGGCGGACCAGTCCTTGCGACCCCGAACCCCTGCGCGCGCCAGGCGCTAAACCACCCGTGAggcg                                                                                                                               >  2:816203/1‑148 (MQ=255)
                   tcattcatTTATTCCTTTATCCAGTCGTTTAAATTTTTATTTTGG‑AGTCAAACCCGCCCAGACACGGCCCACTTGCACAAAGGCGGACCCCTCCAGGCGCCCCCGCCCCCCAGCGCGCGCCCGGCGAGAAACCAACCCAGAGGCGGcc                                                                                                                            >  1:2332110/1‑148 (MQ=255)
                   tcattcatTTATTCCTTTATCCAGTCG‑TTACATTTTTACTTTTGCAGTCAACCCGGCACAGACACCGACCAGTTACAGAATTGCGGACCAGTCCTGGCGACCGCCACCCGCAGCGCGCGCCTGGCGCTAAACCTCCCGTGAGGCGGcc                                                                                                                            >  2:1563265/1‑148 (MQ=255)
                   tcattcatTTATTCCTTTATCCAGTCG‑TTACATTTTTAATTTTGCAGTCATACCGGCACTGACACGGACCTGTTCCAGAATGGCGGTCCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCAGGCGATAAACCTCCCGTGAGGCGGcc                                                                                                                            >  2:1844192/1‑148 (MQ=255)
                   tcattcatTTATTCCTTTATCCAGTCG‑TTACAATTTTAATTTTGCAGTAAACCCCGCACAGACACCGACCAGTTACAGAATTGCGGACCAGTCCAGGCGAACCCGACCCCCGGCGCGCGCCAGGCGATAAAACACCCCTGATGCGGcc                                                                                                                            >  1:1586814/1‑148 (MQ=255)
                      ttcattTATTCCTTTATCCAGTCG‑TTACAATTTTTATTTTGCACTCATCCCGGCACAGACAACGACCTGTTGCACAATGGCGGTCCACTACTGGCGACCGCGACCCGCGGCGCCCGCCAGGCCATAAACCACCCCTGAGGCGGccccc                                                                                                                         >  1:3177307/1‑148 (MQ=255)
                      ttcattTATTCCTTTATCCAGTCG‑TTAAATTTTTAATTTTGCATTAAAACCGGCACTGACACCGACCTGTTCCAGAATGGCGGACCAGTCCAGGCGACCGCGACCCGCAGCGCGCGCCTGGCGCTAAACCACCCCTGAGGCGGccccc                                                                                                                         >  2:3213919/1‑148 (MQ=255)
                       tcattTATTCCTTTATCCAGTCG‑TTCCATTTTTACTTTTGCAGTCAACCCGGCACAGACACGGACCTGTTCCAGAAAGGCGGACCAGTCCTGGCGACCGCGACCCCCGGCGCGCGCCTGGCGATAAACCACCCCTGAGGCGGCCCCCa                                                                                                                        >  2:1348243/1‑148 (MQ=255)
                        cattTATTCCTTTATCCAGTCG‑TTAAATTTTTAATTTTGCAGTCATACCCGCACTGACACCGACCAGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAAACACCCCTGAGGCGGCCCCCAg                                                                                                                       >  2:1033516/1‑148 (MQ=255)
                               tCCTTTATCCAGTCG‑TTAAATTTTTAATTTTGCAGTCATCCCCCCCCAGACACGGACCTGTTACAGAATTGCGGACCAGTCCTGTCGACCCCGACCCCCATCGCGCGCCAGGCCATAAACCACCCCAGAGGCGGCCCCCAGCGGCAtt                                                                                                                >  2:1053232/1‑148 (MQ=255)
                                     aTCCAGTCG‑TTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGcaca                                                                                                          <  2:1759944/148‑1 (MQ=255)
                                     aTCCAGTCG‑TTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGcaca                                                                                                          <  2:1760156/148‑1 (MQ=255)
                                          gTCG‑TTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACCTGCAg                                                                                                     >  2:363413/1‑148 (MQ=255)
                                          gTCG‑TTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAg                                                                                                     <  1:2474970/148‑1 (MQ=255)
                                          gTCG‑TTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAg                                                                                                     >  2:38717/1‑148 (MQ=255)
                                           tCG‑TTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGc                                                                                                    <  2:1914046/148‑1 (MQ=255)
                                           tCG‑TTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGc                                                                                                    >  1:2803039/1‑148 (MQ=255)
                                               ttACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGAGGCATTGGCACAGGCCGCTgg                                                                                                 >  1:1963774/1‑148 (MQ=255)
                                                tACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGt                                                                                                <  2:2676197/148‑1 (MQ=255)
                                                tACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGt                                                                                                >  2:2038924/1‑148 (MQ=255)
                                                tACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGt                                                                                                <  1:1103245/148‑1 (MQ=255)
                                                tACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGt                                                                                                >  1:1693120/1‑148 (MQ=255)
                                                 aCATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGtt                                                                                               <  2:1980889/148‑1 (MQ=255)
                                                  cATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTg                                                                                              >  2:2461957/1‑148 (MQ=255)
                                                   aTTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTgg                                                                                             >  1:3462756/1‑148 (MQ=255)
                                                    tttttACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGc                                                                                            >  2:3454200/1‑148 (MQ=255)
                                                    tttttACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGc                                                                                            >  2:2033738/1‑148 (MQ=255)
                                                     ttttACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCg                                                                                           >  1:2839943/1‑148 (MQ=255)
                                                     ttttACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCg                                                                                           >  1:2839890/1‑148 (MQ=255)
                                                         aCTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACAt                                                                                       <  1:3763256/148‑1 (MQ=255)
                                                          cTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGTACATGCAGCTGGTTGGCGACATc                                                                                      >  2:1350560/1‑148 (MQ=255)
                                                          cTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATc                                                                                      <  1:1642677/148‑1 (MQ=255)
                                                          cTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATc                                                                                      <  1:1642960/148‑1 (MQ=255)
                                                          cTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATc                                                                                      <  1:1642978/148‑1 (MQ=255)
                                                           tttGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCa                                                                                     >  2:582519/1‑148 (MQ=255)
                                                           tttGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCa                                                                                     >  2:187468/1‑148 (MQ=255)
                                                            ttGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCaa                                                                                    >  1:224976/1‑148 (MQ=255)
                                                            ttGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCaa                                                                                    >  1:225066/1‑148 (MQ=255)
                                                             tGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGTGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAg                                                                                   <  1:2772246/148‑1 (MQ=255)
                                                             tGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAg                                                                                   <  1:2772612/148‑1 (MQ=255)
                                                               gCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAggg                                                                                 >  1:767810/1‑148 (MQ=255)
                                                                 aGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCg                                                                               <  2:3683710/148‑1 (MQ=255)
                                                                     aTCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGc                                                                           <  2:2779613/148‑1 (MQ=255)
                                                                     aTCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGc                                                                           <  2:1242641/148‑1 (MQ=255)
                                                                     aTCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGc                                                                           <  2:1746888/148‑1 (MQ=255)
                                                                      tCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGcc                                                                          >  1:3175104/1‑148 (MQ=255)
                                                                       cccGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCa                                                                         <  2:3350168/148‑1 (MQ=255)
                                                                         cGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAg                                                                       >  1:1018134/1‑148 (MQ=255)
                                                                         cGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAg                                                                       <  2:3741375/148‑1 (MQ=255)
                                                                         cGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAg                                                                       >  1:1563106/1‑148 (MQ=255)
                                                                         cGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAg                                                                       <  2:295513/148‑1 (MQ=255)
                                                                         cGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAg                                                                       <  2:924919/148‑1 (MQ=255)
                                                                         cGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAg                                                                       <  1:1836473/148‑1 (MQ=255)
                                                                                  cacGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATg                                                              <  2:1274156/148‑1 (MQ=255)
                                                                                      gACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCa                                                          <  1:437974/148‑1 (MQ=255)
                                                                                      gACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCa                                                          >  2:3283812/1‑148 (MQ=255)
                                                                                      gACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCa                                                          <  2:2899770/148‑1 (MQ=255)
                                                                                      gACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCa                                                          >  2:1460920/1‑148 (MQ=255)
                                                                                       aCCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCac                                                         >  1:3106888/1‑147 (MQ=255)
                                                                                        ccTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAg                                                        <  2:83880/148‑1 (MQ=255)
                                                                                          tGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgat                                                      <  1:1911208/148‑1 (MQ=255)
                                                                                          tGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgat                                                      >  1:2795604/1‑148 (MQ=255)
                                                                                          tGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgat                                                      <  2:855015/148‑1 (MQ=255)
                                                                                             tCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCTTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatcga                                                   >  1:681428/1‑148 (MQ=255)
                                                                                             tCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatcga                                                   >  1:455517/1‑148 (MQ=255)
                                                                                             tCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatcga                                                   <  1:287480/148‑1 (MQ=255)
                                                                                               cAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACgatcgatc                                                 >  1:2559650/1‑148 (MQ=255)
                                                                                               cAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatcgatc                                                 <  1:1563265/148‑1 (MQ=255)
                                                                                                aGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCa                                                <  2:1313685/148‑1 (MQ=255)
                                                                                                 gAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCAt                                               <  1:2710039/148‑1 (MQ=255)
                                                                                                 gAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCAt                                               <  1:1417981/148‑1 (MQ=255)
                                                                                                 gAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCAt                                               <  2:2485025/148‑1 (MQ=255)
                                                                                                   aTGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGa                                             <  2:3160135/148‑1 (MQ=255)
                                                                                                    tGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGaa                                            >  2:3127101/1‑148 (MQ=255)
                                                                                                    tGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGaa                                            <  1:885686/148‑1 (MQ=255)
                                                                                                     ggCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAg                                           <  1:2630894/148‑1 (MQ=255)
                                                                                                            cAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATCATGAAGGCGGgag                                    >  1:2125770/1‑148 (MQ=255)
                                                                                                               tCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGaaa                                 >  1:2006552/1‑148 (MQ=255)
                                                                                                                  tGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAt                              <  1:2536974/148‑1 (MQ=255)
                                                                                                                  tGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAt                              <  1:2536649/148‑1 (MQ=255)
                                                                                                                       accgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCg                         <  1:3627867/148‑1 (MQ=255)
                                                                                                                             accCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAgg                   <  1:1348066/148‑1 (MQ=255)
                                                                                                                              cccGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGa                  <  1:970232/148‑1 (MQ=255)
                                                                                                                                cgcgGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACt                <  2:3073684/148‑1 (MQ=255)
                                                                                                                                      gcgcgcCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACtttgttt          <  2:1699810/148‑1 (MQ=255)
                                                                                                                                          gcCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGcc      <  2:3149593/148‑1 (MQ=255)
                                                                                                                                            cTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCCAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAg    >  1:3306202/1‑148 (MQ=255)
                                                                                                                                              ggCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGa  >  1:3414232/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCG‑TTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGA  >  CP009273/4065989‑4066277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGG  >  CP009273/4065989‑4066282
                                                                                                                                                |                                                                                                                                                     
TCGTTGATGGTGTACTTAGTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAA                                                                                                                                                  <  SRR19973069.413082/150‑1 (MQ=60)
       TGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCG                                                                                                                                           <  SRR19973069.204572/150‑1 (MQ=60)
       TGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCG                                                                                                                                           <  SRR19973069.204624/150‑1 (MQ=60)
       TGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCG                                                                                                                                           <  SRR19973069.873598/150‑1 (MQ=60)
            TACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATGAACCTCCCGTGAGG                                                                                                                                      <  SRR19973069.3181919/150‑1 (MQ=60)
              CTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCG                                                                                                                                    <  SRR19973069.612331/150‑1 (MQ=60)
              CTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCG                                                                                                                                    <  SRR19973069.612084/150‑1 (MQ=60)
                 ATTCATTCATTTATTCCTTTATCCAGTCGTTTCATTTTTACTTTTGCAGTCAACCCCGCACAGACAACGACCTGTTACACAATGGCGGACCAGTCCAGGCGACCGCGCCCCGCGGCGCGCGCCTGGCGATAAAACACCCGTGAGGCGGCC                                                                                                                                 >  SRR19973069.2348001/1‑150 (MQ=60)
                 ATTCATTCATTTATTCCTTTATCCAGTCGTTACAATTTTAATTTTGCAGTAAACCCCGCACAGACACCGACCAGTTACAGAATTGCGGACCAGTCCAGGCGAACCCGACCCCCGGCGCGCGCCAGGCGATAAAACACCCCTGATGCGGCC                                                                                                                                 >  SRR19973069.1597563/1‑150 (MQ=28)
                    CATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACATTGGCAGTCAACCCCGCACAGACACCGACCAGTTACAGAATGGCGGACCAGTCCAGGCGACCGCGACCCGCAGCGCGCCCCAGGCGATAAAACACCCCTGATGCGGCCCCC                                                                                                                              >  SRR19973069.1282710/1‑150 (MQ=60)
                    CATTCATTTATTCCTTTATCCAGTCGTTACATTTTTAATTTGGCAGTAATCCCGGCACAGACAACGACCTGTTCCAGAAATGCGGACCACTCCAGGCGACCGCGCCCCCCGGCGCGCGCCAGGCGCTAAACCAACCCTGAGGCGGCCCCC                                                                                                                              >  SRR19973069.1582729/1‑150 (MQ=53)
                    CATTCATTTATTCCTTTATCCAGTCGTTACAATTTTTATTTTGCACTCATCCCGGCACAGACAACGACCTGTTGCACAATGGCGGTCCACTACTGGCGACCGCGACCCGCGGCGCCCGCCAGGCCATAAACCACCCCTGAGGCGGCCCCC                                                                                                                              >  SRR19973069.3197976/1‑150 (MQ=45)
                        CATTTATTCCTTTATCCAGTCGTTTCATTTTTAATTTTGCAGTCCTCCCCGCACAGACACCGACCAGTTCCAGAAATGCGGGCCACTCCTGGCGACCCCGACCCCCGGCGCCCGCCCGGCGATAAAACACCCGGGAGGCGGCCCCCcccc                                                                                                                          >  SRR19973069.1391258/1‑146 (MQ=26)
                              TTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTG                                                                                                                    <  SRR19973069.2561848/150‑1 (MQ=60)
                                        CAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAG                                                                                                          <  SRR19973069.28645/150‑1 (MQ=60)
                                        CAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAG                                                                                                          <  SRR19973069.734652/150‑1 (MQ=60)
                                         AGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGC                                                                                                         >  SRR19973069.2821642/1‑150 (MQ=60)
                                          GTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCT                                                                                                        <  SRR19973069.2491562/150‑1 (MQ=60)
                                            CGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGAGGCATTGGCACAGGCCGCTGG                                                                                                      >  SRR19973069.1977395/1‑150 (MQ=60)
                                             GTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGT                                                                                                     >  SRR19973069.1704904/1‑150 (MQ=60)
                                             GTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGT                                                                                                     >  SRR19973069.1704947/1‑150 (MQ=60)
                                               TACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTG                                                                                                   <  SRR19973069.1110950/150‑1 (MQ=60)
                                                ACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGG                                                                                                  >  SRR19973069.3485173/1‑150 (MQ=60)
                                                ACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGG                                                                                                  >  SRR19973069.2328190/1‑150 (MQ=60)
                                                 CATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGC                                                                                                 >  SRR19973069.3514700/1‑150 (MQ=60)
                                                  ATTTTTCCTTGGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCG                                                                                                <  SRR19973069.1650508/150‑1 (MQ=60)
                                                  ATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCG                                                                                                >  SRR19973069.2858626/1‑150 (MQ=60)
                                                  ATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCG                                                                                                >  SRR19973069.2858679/1‑150 (MQ=60)
                                                  ATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCG                                                                                                >  SRR19973069.932378/1‑150 (MQ=60)
                                                    TTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGAC                                                                                              >  SRR19973069.2672387/1‑150 (MQ=60)
                                                        ACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCA                                                                                          <  SRR19973069.3787672/150‑1 (MQ=60)
                                                        ACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCA                                                                                          <  SRR19973069.3787721/150‑1 (MQ=60)
                                                         CTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAA                                                                                         <  SRR19973069.1654276/150‑1 (MQ=60)
                                                         CTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAA                                                                                         <  SRR19973069.1654258/150‑1 (MQ=60)
                                                         CTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAA                                                                                         <  SRR19973069.1654239/150‑1 (MQ=60)
                                                         CTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAA                                                                                         <  SRR19973069.1653975/150‑1 (MQ=60)
                                                         CTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAA                                                                                         >  SRR19973069.3236663/1‑150 (MQ=60)
                                                         CTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAA                                                                                         >  SRR19973069.2413037/1‑150 (MQ=60)
                                                         CTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAA                                                                                         >  SRR19973069.226805/1‑150 (MQ=60)
                                                         CTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAA                                                                                         >  SRR19973069.226714/1‑150 (MQ=60)
                                                          TTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAG                                                                                        >  SRR19973069.3003086/1‑150 (MQ=60)
                                                            TGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGTGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGG                                                                                      <  SRR19973069.2790715/150‑1 (MQ=60)
                                                            TGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGG                                                                                      >  SRR19973069.773232/1‑150 (MQ=60)
                                                            TGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGG                                                                                      <  SRR19973069.2791085/150‑1 (MQ=60)
                                                            TGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGG                                                                                      <  SRR19973069.2790692/150‑1 (MQ=60)
                                                            TGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGG                                                                                      <  SRR19973069.2585567/150‑1 (MQ=60)
                                                                  TCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGC                                                                                <  SRR19973069.286850/150‑1 (MQ=60)
                                                                   CATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCC                                                                               >  SRR19973069.3195767/1‑150 (MQ=60)
                                                                      CCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAG                                                                            >  SRR19973069.1025169/1‑150 (MQ=60)
                                                                      CCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAG                                                                            >  SRR19973069.1573770/1‑150 (MQ=60)
                                                                        CGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGAT                                                                          <  SRR19973069.1849322/150‑1 (MQ=60)
                                                                                    GGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAC                                                              >  SRR19973069.3127327/1‑150 (MQ=60)
                                                                                     GACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCCAG                                                             >  SRR19973069.3368/1‑150 (MQ=60)
                                                                                     GACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAG                                                             >  SRR19973069.1569728/1‑150 (MQ=60)
                                                                                     GACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAG                                                             <  SRR19973069.441181/150‑1 (MQ=60)
                                                                                       CCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGAT                                                           >  SRR19973069.2814180/1‑150 (MQ=60)
                                                                                       CCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGAT                                                           >  SRR19973069.2814210/1‑150 (MQ=60)
                                                                                       CCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGAT                                                           >  SRR19973069.2814181/1‑150 (MQ=60)
                                                                                         TGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCG                                                         <  SRR19973069.1924393/150‑1 (MQ=60)
                                                                                          GTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCTTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGA                                                        >  SRR19973069.686163/1‑150 (MQ=60)
                                                                                          GTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGA                                                        >  SRR19973069.458857/1‑150 (MQ=60)
                                                                                          GTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGA                                                        >  SRR19973069.456670/1‑150 (MQ=60)
                                                                                            TCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATC                                                      >  SRR19973069.2576549/1‑150 (MQ=60)
                                                                                            TCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATC                                                      <  SRR19973069.289466/150‑1 (MQ=60)
                                                                                              CAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCAT                                                    <  SRR19973069.1573930/150‑1 (MQ=60)
                                                                                                GAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGA                                                  <  SRR19973069.2728206/150‑1 (MQ=60)
                                                                                                GAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGA                                                  <  SRR19973069.1427878/150‑1 (MQ=60)
                                                                                                   TGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGG                                               <  SRR19973069.891808/150‑1 (MQ=60)
                                                                                                    GGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGC                                              <  SRR19973069.2648509/150‑1 (MQ=60)
                                                                                                        GACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGA                                          <  SRR19973069.1940637/150‑1 (MQ=60)
                                                                                                         ACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATCATGAAGGCGGGAG                                         >  SRR19973069.2140398/1‑150 (MQ=60)
                                                                                                            AGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGACA                                      >  SRR19973069.2020688/1‑150 (MQ=60)
                                                                                                            AGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAA                                      >  SRR19973069.2020518/1‑150 (MQ=60)
                                                                                                                 TGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCT                                 >  SRR19973069.1869625/1‑150 (MQ=60)
                                                                                                                 TGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCT                                 <  SRR19973069.2553453/150‑1 (MQ=60)
                                                                                                                 TGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCT                                 <  SRR19973069.2553779/150‑1 (MQ=60)
                                                                                                                    CGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCG                              <  SRR19973069.3477237/150‑1 (MQ=60)
                                                                                                                      ACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCT                            <  SRR19973069.3651584/150‑1 (MQ=60)
                                                                                                                            ACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGAC                      <  SRR19973069.1357573/150‑1 (MQ=60)
                                                                                                                            ACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGAC                      <  SRR19973069.1357396/150‑1 (MQ=60)
                                                                                                                             CCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACT                     <  SRR19973069.976707/150‑1 (MQ=60)
                                                                                                                               CGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTT                   <  SRR19973069.1756106/150‑1 (MQ=60)
                                                                                                                                  TGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTT                <  SRR19973069.859752/150‑1 (MQ=60)
                                                                                                                                         GCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCCAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAG         >  SRR19973069.3327858/1‑150 (MQ=60)
                                                                                                                                           CTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGA       >  SRR19973069.3436477/1‑150 (MQ=60)
                                                                                                                                              GCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGT    <  SRR19973069.2319872/150‑1 (MQ=60)
                                                                                                                                               CGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGTACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTG   <  SRR19973069.1359914/150‑1 (MQ=60)
                                                                                                                                               CGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGTACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTG   <  SRR19973069.1359913/150‑1 (MQ=60)
                                                                                                                                               CGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTG   >  SRR19973069.1790979/1‑150 (MQ=60)
                                                                                                                                                GATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGG  >  SRR19973069.3844875/1‑150 (MQ=60)
                                                                                                                                                GATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGG  >  SRR19973069.3844789/1‑150 (MQ=60)
                                                                                                                                                GATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGG  <  SRR19973069.847524/150‑1 (MQ=60)
                                                                                                                                                GATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGG  <  SRR19973069.971370/150‑1 (MQ=60)
                                                                                                                                                |                                                                                                                                                     
TCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTGG  >  CP009273/4065989‑4066282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑