Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,066,133 T→G 100% S267R (AGC→CGC)  yihU ← gamma‑hydroxybutyrate dehydrogenase, NADH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,066,1330TG98.8% 1059.5 / ‑1.6 262S267R (AGC→CGC) yihUgamma‑hydroxybutyrate dehydrogenase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/4);  new base G (124/134);  total (124/138)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 264 total aligned reads displayed.

ATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAA  >  CP009273/4065986‑4066278
                                                                                                                                                   |                                                                                                                                                 
    cGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAAc                                                                                                                                             <  1:1666533/150‑1 (MQ=255)
    cGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCAGCGCGCGCCTGGCGATAAAc                                                                                                                                             >  2:2723800/1‑150 (MQ=255)
     gTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAAcc                                                                                                                                            >  2:237809/1‑150 (MQ=255)
     gTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAAcc                                                                                                                                            <  1:2808330/150‑1 (MQ=255)
          tGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACTGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCg                                                                                                                                       <  1:440497/150‑1 (MQ=255)
          tGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCg                                                                                                                                       <  2:2811643/150‑1 (MQ=255)
           ggTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGTCACGGACCTGTTACAGAATGGCGGACCAGTCCAGGCGACCGCGACCCCCTGCGCGCGCCTGGCGATCAACCTCCCGt                                                                                                                                      >  2:747285/1‑150 (MQ=255)
             tgtACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGa                                                                                                                                    >  2:1650877/1‑150 (MQ=255)
              gtACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAg                                                                                                                                   >  2:2413222/1‑150 (MQ=255)
              gtACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACAATTTTACTTTGGCAGTCATACCGGCACAGACACGGACCTGTTCCAGAAATGCGGACCAGTCCAGGCGACCCCCACCCGCAGCGCGCGCCAGGCGATAAACCACCCCTGAg                                                                                                                                   >  1:1833281/1‑150 (MQ=255)
                 cTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAggcg                                                                                                                                <  2:2372372/150‑1 (MQ=255)
                    attcattcATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGcc                                                                                                                             <  1:1751962/150‑1 (MQ=255)
                     ttcattcaTTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACTGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGccc                                                                                                                            <  1:765363/150‑1 (MQ=255)
                     ttcattcaTTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGccc                                                                                                                            <  1:3095347/150‑1 (MQ=255)
                          tcattTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGc                                                                                                                       >  2:2634770/1‑150 (MQ=255)
                            attTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCCGCACAGACACGGACCGGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCACCCGTGAGGCGGCCCCCAGCgg                                                                                                                     >  1:3632029/1‑150 (MQ=255)
                            attTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTAATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCgg                                                                                                                     >  1:2653117/1‑150 (MQ=255)
                               tATTCCTTTATCCAGTCGTTACATTTTTACTTTTGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATTGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCaa                                                                                                                  >  2:34447/1‑149 (MQ=255)
                               tATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCAt                                                                                                                  >  2:1944695/1‑150 (MQ=255)
                                 ttCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTg                                                                                                                <  1:2556904/150‑1 (MQ=255)
                                 ttCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCAGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCACCCGTGAGGCGGCCCCCAGCGGCATTg                                                                                                                >  2:3994323/1‑150 (MQ=255)
                                   ccTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGc                                                                                                              <  1:857940/150‑1 (MQ=255)
                                          ccAGTCTTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCa                                                                                                       <  1:2800344/150‑1 (MQ=255)
                                           cAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACTGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAg                                                                                                      >  1:1978339/1‑150 (MQ=255)
                                            aGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGc                                                                                                     >  2:445713/1‑150 (MQ=255)
                                                gTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGt                                                                                                 >  2:3936321/1‑150 (MQ=255)
                                                  tACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTg                                                                                               >  1:2263051/1‑150 (MQ=255)
                                                     aTTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCg                                                                                            <  1:800764/150‑1 (MQ=255)
                                                         ttACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACAt                                                                                        <  2:2683122/150‑1 (MQ=255)
                                                           aCTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTTGCACATGCAGCTGGTTGGCGACATct                                                                                      <  2:1786489/150‑2 (MQ=255)
                                                            cTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCaa                                                                                     >  2:1125651/1‑150 (MQ=255)
                                                            cTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCaa                                                                                     <  1:1862103/150‑1 (MQ=255)
                                                              ttGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAgg                                                                                   <  2:3579900/150‑1 (MQ=255)
                                                               tGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAggg                                                                                  <  2:2064770/150‑1 (MQ=255)
                                                               tGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAggg                                                                                  <  1:3066663/150‑1 (MQ=255)
                                                               tGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAggg                                                                                  <  1:1213482/150‑1 (MQ=255)
                                                                 gCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCg                                                                                <  2:1311807/150‑1 (MQ=255)
                                                                      cATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGcc                                                                           <  1:3546862/150‑1 (MQ=255)
                                                                       aTCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCa                                                                          >  2:2425741/1‑150 (MQ=255)
                                                                       aTCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCa                                                                          >  1:1950675/1‑150 (MQ=255)
                                                                       aTCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCa                                                                          >  2:1447178/1‑150 (MQ=255)
                                                                         cccGGCACTGACACGGACCTGTTCCAGACTGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAg                                                                        <  1:661621/150‑1 (MQ=255)
                                                                         cccGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAg                                                                        <  2:2318634/150‑1 (MQ=255)
                                                                           cGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGAt                                                                      >  1:373590/1‑150 (MQ=255)
                                                                           cGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGAt                                                                      <  1:3446818/150‑1 (MQ=255)
                                                                            ggCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATc                                                                     >  2:3222247/1‑150 (MQ=255)
                                                                              cACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCt                                                                   <  1:3829354/150‑1 (MQ=255)
                                                                              cACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCt                                                                   <  1:2160907/150‑1 (MQ=255)
                                                                              cACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCt                                                                   >  1:1444495/1‑150 (MQ=255)
                                                                               aCTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCtt                                                                  <  2:1177442/150‑1 (MQ=255)
                                                                                cTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTa                                                                 <  2:137238/150‑1 (MQ=255)
                                                                                cTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTa                                                                 <  1:707226/150‑1 (MQ=255)
                                                                                cTGACACGGACCTGGTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTa                                                                 <  2:2807768/150‑1 (MQ=255)
                                                                                 tGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTAt                                                                >  2:4000672/1‑150 (MQ=255)
                                                                                 tGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTAt                                                                <  2:3844741/150‑1 (MQ=255)
                                                                                  gACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATg                                                               <  2:3376764/150‑1 (MQ=255)
                                                                                  gACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATg                                                               <  2:2263051/150‑1 (MQ=255)
                                                                                    cacGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATggg                                                             <  1:553403/150‑1 (MQ=255)
                                                                                     acGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGc                                                            >  2:1057696/1‑150 (MQ=255)
                                                                                     acGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGc                                                            >  1:616583/1‑150 (MQ=255)
                                                                                       ggACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCaa                                                          <  1:1508212/150‑1 (MQ=255)
                                                                                       ggACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCaa                                                          >  2:257716/1‑150 (MQ=255)
                                                                                       ggACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCaa                                                          >  2:3661704/1‑150 (MQ=255)
                                                                                        gACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAg                                                         >  1:3844859/1‑150 (MQ=255)
                                                                                         aCCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGACTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAga                                                        >  2:789487/1‑150 (MQ=255)
                                                                                            tGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatcg                                                     >  1:2447022/1‑150 (MQ=255)
                                                                                               tCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACgatcgatc                                                  >  1:223564/1‑150 (MQ=255)
                                                                                                ccAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCa                                                 <  2:1524290/150‑1 (MQ=255)
                                                                                                 cAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCAt                                                <  2:2751415/150‑1 (MQ=255)
                                                                                                        gCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCg                                         <  2:3045911/150‑1 (MQ=255)
                                                                                                          ggACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATCATGAAGGCggg                                       >  2:1305446/1‑150 (MQ=255)
                                                                                                          ggACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCggg                                       >  1:2371791/1‑150 (MQ=255)
                                                                                                             ccAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGgaga                                    <  1:138016/150‑1 (MQ=255)
                                                                                                             ccAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGgaga                                    <  1:1151511/150‑1 (MQ=255)
                                                                                                              cAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGaa                                   <  1:2653000/150‑1 (MQ=255)
                                                                                                               aGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCCCGATCGATCATGAAGGCGGGAGaaa                                  >  2:2954485/1‑150 (MQ=255)
                                                                                                               aGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGaaa                                  <  2:3327015/150‑1 (MQ=255)
                                                                                                                gTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAg                                 <  1:2870767/150‑1 (MQ=255)
                                                                                                                gTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAg                                 <  2:3943404/150‑1 (MQ=255)
                                                                                                                  ccTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGAAAGATCGATCATGAAGGCGGGAGAAAGAt                               <  1:920646/150‑1 (MQ=255)
                                                                                                                    tGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAtct                             >  1:3907943/1‑150 (MQ=255)
                                                                                                                    tGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAtct                             <  2:84383/150‑1 (MQ=255)
                                                                                                                     ggCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAtctc                            <  1:329140/150‑1 (MQ=255)
                                                                                                                        gaccgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATCATGAAGGCGGGAGAAAGATCTCCGc                         >  2:1697043/1‑150 (MQ=255)
                                                                                                                        gaccgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGc                         >  1:4054693/1‑150 (MQ=255)
                                                                                                                        gaccgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGc                         >  1:1715498/1‑150 (MQ=255)
                                                                                                                          ccgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTg                       <  2:189033/150‑1 (MQ=255)
                                                                                                                              gaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGa                   <  1:3358309/150‑1 (MQ=255)
                                                                                                                                  cGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACttt               <  1:1808329/150‑1 (MQ=255)
                                                                                                                                   gCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACtttg              <  1:4120358/150‑1 (MQ=255)
                                                                                                                                     tGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACtttgtt            <  2:954083/150‑1 (MQ=255)
                                                                                                                                     tGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACtttgtt            <  2:9815/150‑1 (MQ=255)
                                                                                                                                      gcgcgcgcCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACtttgttt           <  2:574004/150‑1 (MQ=255)
                                                                                                                                        gcgcgcCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTgg         <  1:2771049/150‑1 (MQ=255)
                                                                                                                                         cgcgcCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGc        >  2:68126/1‑150 (MQ=255)
                                                                                                                                          gcgcCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGcc       <  2:1213892/150‑1 (MQ=255)
                                                                                                                                           cgcCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCa      <  2:3500564/150‑1 (MQ=255)
                                                                                                                                            gcCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAg     <  2:3140845/150‑1 (MQ=255)
                                                                                                                                             ccTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAgg    >  2:1047618/1‑150 (MQ=255)
                                                                                                                                               tGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGaa  >  2:7133/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAA  >  CP009273/4065986‑4066278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTG  >  CP009273/4065987‑4066281
                                                                                                                                                  |                                                                                                                                                    
TGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTAATTTGGCAGTCATCCCGGCACATACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCAGGCGATA                                                                                                                                                   >  SRR19973074.955896/1‑150 (MQ=60)
   CGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAAC                                                                                                                                                <  SRR19973074.1669675/150‑1 (MQ=60)
    GTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACC                                                                                                                                               <  SRR19973074.2813498/150‑1 (MQ=60)
    GTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACC                                                                                                                                               <  SRR19973074.2813516/150‑1 (MQ=60)
     TTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCT                                                                                                                                              <  SRR19973074.3432812/150‑1 (MQ=60)
        TTGGTGTTCTTATTAATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCC                                                                                                                                           <  SRR19973074.2479016/150‑1 (MQ=60)
        ATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCC                                                                                                                                           <  SRR19973074.3479207/150‑1 (MQ=60)
        ATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCC                                                                                                                                           <  SRR19973074.917269/150‑1 (MQ=60)
         TGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACTGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCG                                                                                                                                          <  SRR19973074.441348/150‑1 (MQ=60)
             GTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACAATTTTACTTTGGCAGTCATACCGGCACAGACACGGACCTGTTCCAGAAATGCGGACCAGTCCAGGCGACCCCCACCCGCAGCGCGCGCCAGGCGATAAACCACCCCTGAG                                                                                                                                      >  SRR19973074.1836778/1‑150 (MQ=60)
                CTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCAACCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGAACGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCG                                                                                                                                   >  SRR19973074.3736341/1‑150 (MQ=60)
                CTTATTCATTCATTTATTCCTTTATCCAGTCGTGACATTTTTAATTTTGCAGTCATCCCGGCACTGACACGGACCAGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCCCTGCGCGCGCCTGGCGATAAAACACCCCTGAGGCG                                                                                                                                   >  SRR19973074.2794299/1‑150 (MQ=60)
                  TATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGC                                                                                                                                 <  SRR19973074.1728116/150‑1 (MQ=60)
                   ATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCC                                                                                                                                <  SRR19973074.1755226/150‑1 (MQ=60)
                    TTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACTGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCC                                                                                                                               <  SRR19973074.766544/150‑1 (MQ=60)
                    TTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACTGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCC                                                                                                                               <  SRR19973074.766825/150‑1 (MQ=60)
                    TTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCC                                                                                                                               <  SRR19973074.3101032/150‑1 (MQ=60)
                        TTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTTGCAGTCATACCGGCACTGACACGGACCGGTTCCAGAATTGCGGACCAGTCCTGGCGACCGCGACCCGCAGCGCGCGCCAGGCGATAAACCTCCCGTGAGGCGGCCCCCAC                                                                                                                           >  SRR19973074.208007/1‑150 (MQ=60)
                           ATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTTGCATTCATCCCGGCACTGACACGGACCTGTTCCAGAATTGCGGACCAGTACTGGCGACCGCGACCCCCGGCGCGCGCCTGGCGATAAAACACCCGTGAGGCGGCCCCCACCGG                                                                                                                        >  SRR19973074.1821186/1‑150 (MQ=60)
                           ATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTTGCAGTAATCCCGGCACAGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCCCCACCCGCTGCGCGCGCCAGGCGATAAACCACCCCTGAGGCGGCCCCCACCGG                                                                                                                        >  SRR19973074.3923894/1‑150 (MQ=60)
                           ATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCCGCACAGACACGGACCGGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCACCCGTGAGGCGGCCCCCAGCGG                                                                                                                        >  SRR19973074.3638743/1‑150 (MQ=60)
                           ATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTAATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGG                                                                                                                        >  SRR19973074.2658062/1‑150 (MQ=60)
                             TTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCA                                                                                                                      <  SRR19973074.2350901/150‑1 (MQ=60)
                              TATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCACCCGTGAGGCGGCCCCCCGCGGCCT                                                                                                                     >  SRR19973074.2704117/1‑150 (MQ=60)
                               ATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCCGCGGCATT                                                                                                                    >  SRR19973074.3752125/1‑150 (MQ=60)
                               ATTCCTTTATCCAGTCGTTAAAATTTTACTTTTGCAGTCATCCCCGCCCAGACACCGACCAGTTCCAGAATGGCGGACCACTCCAGGCGACCGCGACCCCCAGCGCGCGCCAGGCGATAAAACACCCGTGAGGCGGCCCCCCCCGGCATT                                                                                                                    >  SRR19973074.1770842/1‑150 (MQ=55)
                                TTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTG                                                                                                                   <  SRR19973074.2561677/150‑1 (MQ=60)
                                TTCCTTTATCCAGTCGTTACAATTTTACATTGGCAGTCATACCCGCACAGACACCGACCAGTTCCAGAAATGCGGACCAGTCCAGGCGACCGCGACCCGCAGCGCGCGCCTGGCGATAAAACACCCCTGAGGCGGCCCCCACCCGCATTG                                                                                                                   >  SRR19973074.2469244/1‑150 (MQ=60)
                                 TCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGG                                                                                                                  <  SRR19973074.2204604/150‑1 (MQ=60)
                                  CCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGC                                                                                                                 <  SRR19973074.859593/150‑1 (MQ=60)
                                  CCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCCGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCAGGCGACCCCCACCCCCAGCGCGCGCCAGGCGATAAACCTCCCGTGAGGCGGCCCCCCGCGGCAATGGC                                                                                                                 >  SRR19973074.2638458/1‑150 (MQ=60)
                                         CCAGTCTTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCA                                                                                                          <  SRR19973074.2805509/150‑1 (MQ=60)
                                          CAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACTGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAG                                                                                                         >  SRR19973074.1982104/1‑150 (MQ=60)
                                           AGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCAGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGC                                                                                                        >  SRR19973074.1382694/1‑150 (MQ=60)
                                                TTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTT                                                                                                   <  SRR19973074.1755068/150‑1 (MQ=60)
                                                 TACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTG                                                                                                  <  SRR19973074.2345942/150‑1 (MQ=60)
                                                 TACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTG                                                                                                  <  SRR19973074.3099523/150‑1 (MQ=60)
                                                 TACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTG                                                                                                  >  SRR19973074.2267301/1‑150 (MQ=60)
                                                   CATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGC                                                                                                <  SRR19973074.1207349/150‑1 (MQ=60)
                                                   CATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGC                                                                                                <  SRR19973074.431775/150‑1 (MQ=60)
                                                   CATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGC                                                                                                <  SRR19973074.431780/150‑1 (MQ=60)
                                                   CATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGC                                                                                                >  SRR19973074.3492529/1‑150 (MQ=60)
                                                    ATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCG                                                                                               <  SRR19973074.804457/150‑1 (MQ=60)
                                                    ATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCG                                                                                               <  SRR19973074.802302/150‑1 (MQ=60)
                                                     TTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGA                                                                                              <  SRR19973074.1513038/150‑1 (MQ=60)
                                                      TTTTACTTTGGCAGTCATCCCGGCACTGACNCGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGAC                                                                                             >  SRR19973074.3863401/1‑150 (MQ=60)
                                                      TTTTACTTTGGCAGTCATCCCGGCACTGACNCGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGAC                                                                                             >  SRR19973074.3863404/1‑150 (MQ=60)
                                                        TTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACAT                                                                                           <  SRR19973074.1325804/150‑1 (MQ=60)
                                                         TACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATC                                                                                          >  SRR19973074.617612/1‑150 (MQ=60)
                                                          ACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCA                                                                                         >  SRR19973074.3326280/1‑150 (MQ=60)
                                                           CTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAA                                                                                        <  SRR19973074.1865664/150‑1 (MQ=60)
                                                           CTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAA                                                                                        <  SRR19973074.1865663/150‑1 (MQ=60)
                                                           CTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAA                                                                                        >  SRR19973074.1576839/1‑150 (MQ=60)
                                                            TTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAG                                                                                       >  SRR19973074.656633/1‑150 (MQ=60)
                                                              TGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGG                                                                                     <  SRR19973074.978028/150‑1 (MQ=60)
                                                              TGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGG                                                                                     <  SRR19973074.2940859/150‑1 (MQ=60)
                                                              TGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGG                                                                                     <  SRR19973074.2940871/150‑1 (MQ=60)
                                                              TGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGG                                                                                     <  SRR19973074.3072313/150‑1 (MQ=60)
                                                              TGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGG                                                                                     <  SRR19973074.1215810/150‑1 (MQ=60)
                                                                 CAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGA                                                                                  >  SRR19973074.3825245/1‑150 (MQ=60)
                                                                     CATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCC                                                                              <  SRR19973074.3553400/150‑1 (MQ=60)
                                                                     CATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCC                                                                              <  SRR19973074.3553538/150‑1 (MQ=60)
                                                                      ATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCA                                                                             >  SRR19973074.1954444/1‑150 (MQ=60)
                                                                      ATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCA                                                                             >  SRR19973074.1954398/1‑150 (MQ=60)
                                                                      ATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCA                                                                             <  SRR19973074.779453/150‑1 (MQ=60)
                                                                      ATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCA                                                                             <  SRR19973074.779471/150‑1 (MQ=60)
                                                                        CCCGGCACTGACACGGACCTGTTCCAGACTGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAG                                                                           <  SRR19973074.662848/150‑1 (MQ=60)
                                                                        CCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAG                                                                           <  SRR19973074.662860/150‑1 (MQ=60)
                                                                          CGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGAT                                                                         <  SRR19973074.3453158/150‑1 (MQ=60)
                                                                          CGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGAT                                                                         >  SRR19973074.374289/1‑150 (MQ=60)
                                                                             CACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGTGGCGGCCCCCAGAGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCT                                                                      <  SRR19973074.3836404/150‑1 (MQ=60)
                                                                             CACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCT                                                                      <  SRR19973074.3836336/150‑1 (MQ=60)
                                                                             CACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCT                                                                      >  SRR19973074.1447195/1‑150 (MQ=60)
                                                                             CACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCT                                                                      >  SRR19973074.1446978/1‑150 (MQ=60)
                                                                             CACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCT                                                                      <  SRR19973074.2164975/150‑1 (MQ=60)
                                                                              ACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTT                                                                     >  SRR19973074.65766/1‑150 (MQ=60)
                                                                               CTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTA                                                                    <  SRR19973074.708541/150‑1 (MQ=60)
                                                                                   CACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGG                                                                <  SRR19973074.554492/150‑1 (MQ=60)
                                                                                   CACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGG                                                                <  SRR19973074.554511/150‑1 (MQ=60)
                                                                                   CACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGG                                                                <  SRR19973074.2818040/150‑1 (MQ=60)
                                                                                    ACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGC                                                               >  SRR19973074.617750/1‑150 (MQ=60)
                                                                                    ACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGC                                                               <  SRR19973074.2581308/150‑1 (MQ=60)
                                                                                      GGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAA                                                             <  SRR19973074.1511002/150‑1 (MQ=60)
                                                                                      GGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAA                                                             <  SRR19973074.1510976/150‑1 (MQ=60)
                                                                                      GGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAA                                                             <  SRR19973074.1511057/150‑1 (MQ=60)
                                                                                       GACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAG                                                            >  SRR19973074.3851869/1‑150 (MQ=60)
                                                                                          CTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATC                                                         <  SRR19973074.1938755/150‑1 (MQ=60)
                                                                                          CTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATC                                                         >  SRR19973074.3640027/1‑150 (MQ=60)
                                                                                           TGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCG                                                        >  SRR19973074.2451550/1‑150 (MQ=60)
                                                                                              TCCAGAATGGCGGACTAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATC                                                     <  SRR19973074.1757320/150‑1 (MQ=60)
                                                                                              TCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATC                                                     >  SRR19973074.224027/1‑150 (MQ=60)
                                                                                              TCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATC                                                     >  SRR19973074.224104/1‑150 (MQ=60)
                                                                                                CAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCAT                                                   <  SRR19973074.2004432/150‑1 (MQ=60)
                                                                                                 AGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATG                                                  >  SRR19973074.3123693/1‑150 (MQ=60)
                                                                                                         GGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCCCGATCGATCATGAAGGCGGG                                          >  SRR19973074.2376247/1‑150 (MQ=60)
                                                                                                         GGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGG                                          >  SRR19973074.2826773/1‑150 (MQ=60)
                                                                                                         GGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGG                                          >  SRR19973074.2376203/1‑150 (MQ=60)
                                                                                                          GACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGA                                         <  SRR19973074.4017113/150‑1 (MQ=60)
                                                                                                            CCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGA                                       <  SRR19973074.138337/150‑1 (MQ=60)
                                                                                                            CCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGA                                       <  SRR19973074.2801722/150‑1 (MQ=60)
                                                                                                            CCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGA                                       <  SRR19973074.1153705/150‑1 (MQ=60)
                                                                                                            CCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGA                                       <  SRR19973074.50814/150‑1 (MQ=60)
                                                                                                             CAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAA                                      <  SRR19973074.2657967/150‑1 (MQ=60)
                                                                                                             CAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAA                                      <  SRR19973074.2657945/150‑1 (MQ=60)
                                                                                                               GTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAG                                    <  SRR19973074.2876093/150‑1 (MQ=60)
                                                                                                                 CCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGAAAGATCGATCATGAAGGCGGGAGAAAGAT                                  <  SRR19973074.922398/150‑1 (MQ=60)
                                                                                                                   TGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCT                                >  SRR19973074.3915118/1‑150 (MQ=60)
                                                                                                                    GGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTC                               <  SRR19973074.329737/150‑1 (MQ=60)
                                                                                                                      CGACCGCGACCCGCTGCGCGCGCATGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGAGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCG                             <  SRR19973074.2709343/150‑1 (MQ=60)
                                                                                                                       GACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGC                            >  SRR19973074.1718686/1‑150 (MQ=60)
                                                                                                                       GACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGC                            >  SRR19973074.1718666/1‑150 (MQ=60)
                                                                                                                       GACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGC                            >  SRR19973074.4062191/1‑150 (MQ=60)
                                                                                                                       GACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGC                            >  SRR19973074.4062190/1‑150 (MQ=60)
                                                                                                                        ACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCT                           >  SRR19973074.3374040/1‑150 (MQ=60)
                                                                                                                             GACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGA                      <  SRR19973074.3364537/150‑1 (MQ=60)
                                                                                                                                 CGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTT                  <  SRR19973074.1811773/150‑1 (MQ=60)
                                                                                                                                  GCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTG                 <  SRR19973074.4127960/150‑1 (MQ=60)
                                                                                                                                       GCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGG            <  SRR19973074.2776138/150‑1 (MQ=60)
                                                                                                                                         GCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCC          <  SRR19973074.898097/150‑1 (MQ=60)
                                                                                                                                            CCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCCAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGG       >  SRR19973074.1841835/1‑150 (MQ=60)
                                                                                                                                             CTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGA      >  SRR19973074.766244/1‑150 (MQ=60)
                                                                                                                                              TGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAA     >  SRR19973074.2265359/1‑150 (MQ=60)
                                                                                                                                                 CGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTG  >  SRR19973074.1420668/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                    
TGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTG  >  CP009273/4065987‑4066281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑