Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,288,086 G→T 92.6% F366L (TTC→TTA)  fdhF ← formate dehydrogenase‑H, selenopolypeptide subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,288,0860GT92.6% 489.8 / 28.8 176F366L (TTC→TTA) fdhFformate dehydrogenase‑H, selenopolypeptide subunit
Reads supporting (aligned to +/- strand):  ref base G (2/11);  new base T (62/101);  total (64/112)
Fisher's exact test for biased strand distribution p-value = 1.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 176 total aligned reads displayed.

CTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGT  >  CP009273/4287941‑4288229
                                                                                                                                                 |                                                                                                                                               
cTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTaa                                                                                                                                               <  1:222262/148‑1 (MQ=255)
 tGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAAt                                                                                                                                              <  2:666599/148‑1 (MQ=255)
  gCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAAtt                                                                                                                                             >  1:1931360/1‑148 (MQ=255)
   ccGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAAttt                                                                                                                                            <  2:2179582/148‑1 (MQ=255)
   ccGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAAttt                                                                                                                                            <  2:2366603/148‑1 (MQ=255)
   ccGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAAttt                                                                                                                                            >  2:2597932/1‑148 (MQ=255)
     gACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTtct                                                                                                                                          <  2:2498610/148‑1 (MQ=255)
          cTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCgg                                                                                                                                     >  2:3078167/1‑148 (MQ=255)
           tCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGt                                                                                                                                    >  2:2393798/1‑148 (MQ=255)
              gcgTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAg                                                                                                                                 >  2:2970699/1‑148 (MQ=255)
               cgTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGc                                                                                                                                <  1:1050070/148‑1 (MQ=255)
               cgTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGc                                                                                                                                >  2:2921672/1‑148 (MQ=255)
               cgTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGc                                                                                                                                <  1:1184272/148‑1 (MQ=255)
               cgTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGc                                                                                                                                <  2:769233/148‑1 (MQ=255)
                 tCAGTTTGTAGCGGATCGTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCg                                                                                                                              <  2:2345099/148‑1 (MQ=255)
                  cAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCgg                                                                                                                             <  2:2571683/148‑1 (MQ=255)
                  cAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCgg                                                                                                                             >  2:2623779/1‑148 (MQ=255)
                  cAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCgg                                                                                                                             >  1:791539/1‑148 (MQ=255)
                    gTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGAt                                                                                                                           <  1:2442510/148‑1 (MQ=255)
                     tttGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCATGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATc                                                                                                                          >  1:136824/1‑148 (MQ=255)
                       tGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCtt                                                                                                                        <  2:1931360/148‑1 (MQ=255)
                         tAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTc                                                                                                                      >  2:1314389/1‑148 (MQ=255)
                               aTCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTAc                                                                                                                >  2:947964/1‑148 (MQ=255)
                               aTCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTAc                                                                                                                >  2:1294044/1‑148 (MQ=255)
                               aTCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTAc                                                                                                                <  1:1456053/148‑1 (MQ=255)
                                 cTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTg                                                                                                              >  2:2061485/1‑148 (MQ=255)
                                   tCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGt                                                                                                            <  1:2860517/148‑1 (MQ=255)
                                   tCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGt                                                                                                            <  2:3258128/148‑1 (MQ=255)
                                   tCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGt                                                                                                            <  1:3243065/148‑1 (MQ=255)
                                   tCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGAGGTTAGCCGGATCTTTCACGTACTGGt                                                                                                            <  2:1733057/148‑1 (MQ=255)
                                        cATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATccc                                                                                                       >  2:2247099/1‑148 (MQ=255)
                                        cATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATccc                                                                                                       >  2:586223/1‑148 (MQ=255)
                                        cATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATccc                                                                                                       >  1:488282/1‑148 (MQ=255)
                                            aTGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGTCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGAt                                                                                                   <  2:3002087/148‑1 (MQ=255)
                                                aCGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGt                                                                                               <  2:3149018/148‑1 (MQ=255)
                                                 cgcgGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTa                                                                                              <  2:1693373/148‑1 (MQ=255)
                                                  gcgGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTAt                                                                                             <  2:151694/148‑1 (MQ=255)
                                                   cgGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATc                                                                                            <  2:1916391/148‑1 (MQ=255)
                                                   cgGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATc                                                                                            <  2:1916366/148‑1 (MQ=255)
                                                   cgGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATc                                                                                            <  2:1916266/148‑1 (MQ=255)
                                                   cgGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATc                                                                                            <  1:703814/148‑1 (MQ=255)
                                                      cacgcacTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCgg                                                                                         >  2:1399519/1‑148 (MQ=255)
                                                        cgcacTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCa                                                                                       >  2:845074/1‑148 (MQ=255)
                                                         gcacTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAg                                                                                      >  1:1523829/1‑148 (MQ=255)
                                                              tCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAgcgcgc                                                                                 >  1:1198467/1‑148 (MQ=255)
                                                              tCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAgcgcgc                                                                                 >  1:1472968/1‑148 (MQ=255)
                                                                 ccATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCa                                                                              >  1:747742/1‑148 (MQ=255)
                                                                  cATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCat                                                                             >  2:2955383/1‑148 (MQ=255)
                                                                   aTGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCata                                                                            >  1:2785083/1‑148 (MQ=255)
                                                                   aTGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCata                                                                            <  1:35520/148‑1 (MQ=255)
                                                                    tgcgctgcgcGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCatat                                                                           >  2:2214971/1‑148 (MQ=255)
                                                                     gcgctgcgcGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATc                                                                          >  1:2481243/1‑148 (MQ=255)
                                                                              ggTGCGGCAGGTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCAcc                                                                 <  2:3027310/148‑1 (MQ=255)
                                                                              ggTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCAcc                                                                 <  1:11238/148‑1 (MQ=255)
                                                                              ggTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCAcc                                                                 <  1:3024360/148‑1 (MQ=255)
                                                                              ggTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCAcc                                                                 <  2:3026761/148‑1 (MQ=255)
                                                                              ggTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCAcc                                                                 <  1:260165/148‑1 (MQ=255)
                                                                              ggTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCAcc                                                                 <  1:260276/148‑1 (MQ=255)
                                                                               gTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCAccc                                                                <  2:1735379/148‑1 (MQ=255)
                                                                                  cGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGa                                                             <  1:435713/148‑1 (MQ=255)
                                                                                  cGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGa                                                             >  1:673046/1‑148 (MQ=255)
                                                                                     cAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACg                                                          <  1:72862/148‑1 (MQ=255)
                                                                                     cAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACg                                                          <  1:3153831/148‑1 (MQ=255)
                                                                                      aGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACgt                                                         <  2:3191216/148‑1 (MQ=255)
                                                                                      aGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACgt                                                         <  2:1991630/148‑1 (MQ=255)
                                                                                      aGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACgt                                                         <  1:967623/148‑1 (MQ=255)
                                                                                       gCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACgtt                                                        >  2:2141284/1‑148 (MQ=255)
                                                                                        cTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACgttg                                                       <  1:514507/148‑1 (MQ=255)
                                                                                           gCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTc                                                    <  1:2954797/148‑1 (MQ=255)
                                                                                              gATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGa                                                 <  1:1786813/148‑1 (MQ=255)
                                                                                                       gCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAAcc                                        >  2:2214931/1‑148 (MQ=255)
                                                                                                       gCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAAcc                                        <  2:2697508/148‑1 (MQ=255)
                                                                                                        ccGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCg                                       <  2:2750684/148‑1 (MQ=255)
                                                                                                          ggTAGGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCCTATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCggg                                     <  1:3037524/148‑1 (MQ=255)
                                                                                                              tGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTaa                                 <  2:79628/148‑1 (MQ=255)
                                                                                                                cgcTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAaca                               <  2:154943/148‑1 (MQ=255)
                                                                                                                 gcTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAacac                              <  1:2712807/148‑1 (MQ=255)
                                                                                                                  cTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACAcc                             <  2:1262340/148‑1 (MQ=255)
                                                                                                                     gCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGc                          <  1:1046511/148‑1 (MQ=255)
                                                                                                                     gCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGc                          >  2:530743/1‑148 (MQ=255)
                                                                                                                      cAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCa                         >  1:1862034/1‑148 (MQ=255)
                                                                                                                       aGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCAt                        <  1:1613523/148‑1 (MQ=255)
                                                                                                                       aGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCAt                        >  2:1225132/1‑148 (MQ=255)
                                                                                                                        ggCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATg                       <  1:1872624/148‑1 (MQ=255)
                                                                                                                          cTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCg                     >  2:588629/1‑148 (MQ=255)
                                                                                                                          cTTTCCACGCCCCAGGCTTTGGCTAATTACTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGAAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCg                     >  1:3282838/1‑148 (MQ=255)
                                                                                                                            ttCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGc                   >  2:252231/1‑148 (MQ=255)
                                                                                                                             tCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCt                  <  1:3070207/148‑1 (MQ=255)
                                                                                                                                 cGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTAcc              >  1:403679/1‑148 (MQ=255)
                                                                                                                                   ccccAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGa            <  1:177733/148‑1 (MQ=255)
                                                                                                                                     ccAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAgg          <  2:400008/148‑1 (MQ=255)
                                                                                                                                       aGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGtt        >  1:2242313/1‑148 (MQ=255)
                                                                                                                                        ggCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTa       >  1:2020219/1‑148 (MQ=255)
                                                                                                                                         gCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTAc      >  1:28975/1‑148 (MQ=255)
                                                                                                                                         gCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTAc      >  2:1740915/1‑148 (MQ=255)
                                                                                                                                         gCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGGATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTAc      >  2:1740654/1‑148 (MQ=255)
                                                                                                                                          cTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTAcc     <  2:333629/148‑1 (MQ=255)
                                                                                                                                          cTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTAcc     <  2:2096389/148‑1 (MQ=255)
                                                                                                                                           tttGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCg    >  2:732326/1‑148 (MQ=255)
                                                                                                                                             tGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGt  <  1:3078167/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGT  >  CP009273/4287941‑4288229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCA  >  CP009273/4287940‑4288231
                                                                                                                                                  |                                                                                                                                                 
ACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAAT                                                                                                                                                >  SRR19973070.2820340/1‑150 (MQ=60)
 CTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATT                                                                                                                                               <  SRR19973070.223867/150‑1 (MQ=60)
 CTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATT                                                                                                                                               >  SRR19973070.1944363/1‑150 (MQ=60)
              CGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGC                                                                                                                                  >  SRR19973070.488042/1‑150 (MQ=60)
                CGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCG                                                                                                                                <  SRR19973070.1057460/150‑1 (MQ=60)
                CGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCG                                                                                                                                <  SRR19973070.1192597/150‑1 (MQ=60)
                CGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCG                                                                                                                                <  SRR19973070.1193227/150‑1 (MQ=60)
                CGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCG                                                                                                                                <  SRR19973070.3082171/150‑1 (MQ=60)
                 GTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGG                                                                                                                               >  SRR19973070.797020/1‑150 (MQ=60)
                    AGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCATGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATC                                                                                                                            >  SRR19973070.138016/1‑150 (MQ=60)
                     GTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCT                                                                                                                           <  SRR19973070.789817/150‑1 (MQ=60)
                     GTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCT                                                                                                                           <  SRR19973070.2458509/150‑1 (MQ=60)
                      TTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTT                                                                                                                          <  SRR19973070.2738252/150‑1 (MQ=60)
                                ATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTG                                                                                                                <  SRR19973070.2090413/150‑1 (MQ=60)
                                ATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTG                                                                                                                <  SRR19973070.1466202/150‑1 (MQ=60)
                                    TCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTAT                                                                                                            <  SRR19973070.3263975/150‑1 (MQ=60)
                                    TCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTAT                                                                                                            <  SRR19973070.3055877/150‑1 (MQ=60)
                                    TCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTAT                                                                                                            <  SRR19973070.2879067/150‑1 (MQ=60)
                                    TCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTAT                                                                                                            <  SRR19973070.2879055/150‑1 (MQ=60)
                                       CCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCC                                                                                                         >  SRR19973070.491709/1‑150 (MQ=60)
                                                    CGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCG                                                                                            <  SRR19973070.708828/150‑1 (MQ=60)
                                                    CGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCG                                                                                            <  SRR19973070.708851/150‑1 (MQ=60)
                                                        ACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAG                                                                                        >  SRR19973070.1534530/1‑150 (MQ=60)
                                                             CTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGC                                                                                   >  SRR19973070.1483267/1‑150 (MQ=60)
                                                             CTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGC                                                                                   >  SRR19973070.1483258/1‑150 (MQ=60)
                                                             CTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGC                                                                                   >  SRR19973070.1206888/1‑150 (MQ=60)
                                                                CGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCA                                                                                >  SRR19973070.753027/1‑150 (MQ=60)
                                                                  CCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATA                                                                              >  SRR19973070.2803000/1‑150 (MQ=60)
                                                                    ATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATC                                                                            <  SRR19973070.35984/150‑1 (MQ=60)
                                                                    ATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATC                                                                            <  SRR19973070.35985/150‑1 (MQ=60)
                                                                    ATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATC                                                                            >  SRR19973070.2497388/1‑150 (MQ=60)
                                                                    ATGCGCTGCGCGGTGCGGCAGCTCGCTGATGAGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATC                                                                            <  SRR19973070.1511826/150‑1 (MQ=60)
                                                                         CTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGG                                                                       <  SRR19973070.1991092/150‑1 (MQ=60)
                                                                         CTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGG                                                                       <  SRR19973070.1440192/150‑1 (MQ=60)
                                                                               GGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCT                                                                 <  SRR19973070.261905/150‑1 (MQ=60)
                                                                               GGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCT                                                                 <  SRR19973070.3043628/150‑1 (MQ=60)
                                                                               GGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCT                                                                 <  SRR19973070.262016/150‑1 (MQ=60)
                                                                               GGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCT                                                                 <  SRR19973070.11418/150‑1 (MQ=60)
                                                                                 TGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGA                                                               >  SRR19973070.677847/1‑150 (MQ=60)
                                                                                   CGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAAC                                                             <  SRR19973070.438876/150‑1 (MQ=60)
                                                                                      CAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTT                                                          <  SRR19973070.1758302/150‑1 (MQ=60)
                                                                                      CAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTT                                                          <  SRR19973070.73647/150‑1 (MQ=60)
                                                                                      CAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTT                                                          <  SRR19973070.1167302/150‑1 (MQ=60)
                                                                                      CAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTT                                                          <  SRR19973070.3174210/150‑1 (MQ=60)
                                                                                       AGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTG                                                         <  SRR19973070.974532/150‑1 (MQ=60)
                                                                                         CTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTT                                                       <  SRR19973070.518033/150‑1 (MQ=60)
                                                                                           CGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCT                                                     <  SRR19973070.3279500/150‑1 (MQ=60)
                                                                                            GCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTG                                                    <  SRR19973070.2973812/150‑1 (MQ=60)
                                                                                              TGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGAC                                                  <  SRR19973070.2570286/150‑1 (MQ=60)
                                                                                              TGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGAC                                                  <  SRR19973070.3115115/150‑1 (MQ=60)
                                                                                               GATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACC                                                 <  SRR19973070.1799007/150‑1 (MQ=60)
                                                                                               GATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACC                                                 <  SRR19973070.1799168/150‑1 (MQ=60)
                                                                                                       AGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCG                                         <  SRR19973070.3008941/150‑1 (MQ=60)
                                                                                                           GGTAGGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCCTATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTT                                     <  SRR19973070.3056841/150‑1 (MQ=60)
                                                                                                              ATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAAC                                  >  SRR19973070.430831/1‑150 (MQ=60)
                                                                                                                  GCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCC                              <  SRR19973070.1068690/150‑1 (MQ=60)
                                                                                                                  GCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCC                              <  SRR19973070.2730339/150‑1 (MQ=60)
                                                                                                                   CTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCG                             <  SRR19973070.2371878/150‑1 (MQ=60)
                                                                                                                     GGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCA                           >  SRR19973070.1874771/1‑150 (MQ=60)
                                                                                                                      GCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCAT                          <  SRR19973070.2716211/150‑1 (MQ=60)
                                                                                                                      GCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCAT                          <  SRR19973070.1053882/150‑1 (MQ=60)
                                                                                                                       CAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATG                         >  SRR19973070.2480761/1‑150 (MQ=60)
                                                                                                                        AGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGC                        <  SRR19973070.1624759/150‑1 (MQ=60)
                                                                                                                         GGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCG                       <  SRR19973070.1885394/150‑1 (MQ=60)
                                                                                                                         GGCTTTCCACGCCCCAGGCTTTGGCTAATTACTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGAAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCG                       >  SRR19973070.3303921/1‑150 (MQ=60)
                                                                                                                           CTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGC                     <  SRR19973070.57585/150‑1 (MQ=60)
                                                                                                                             TTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTT                   <  SRR19973070.3216515/150‑1 (MQ=60)
                                                                                                                              TCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTA                  <  SRR19973070.3089907/150‑1 (MQ=60)
                                                                                                                               CCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTAC                 <  SRR19973070.181691/150‑1 (MQ=60)
                                                                                                                                CACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACC                >  SRR19973070.406642/1‑150 (MQ=60)
                                                                                                                                    CCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGG            <  SRR19973070.179141/150‑1 (MQ=60)
                                                                                                                                      CCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTT          >  SRR19973070.703314/1‑150 (MQ=60)
                                                                                                                                      CCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTT          >  SRR19973070.2257121/1‑150 (MQ=60)
                                                                                                                                       CAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTA         >  SRR19973070.2033881/1‑150 (MQ=60)
                                                                                                                                        AGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTAC        >  SRR19973070.29362/1‑150 (MQ=60)
                                                                                                                                          GCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCG      <  SRR19973070.1933301/150‑1 (MQ=60)
                                                                                                                                           CTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGG     >  SRR19973070.853854/1‑150 (MQ=60)
                                                                                                                                              TGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCA  >  SRR19973070.706988/1‑150 (MQ=60)
                                                                                                                                              TGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCA  >  SRR19973070.238359/1‑150 (MQ=60)
                                                                                                                                              TGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCA  >  SRR19973070.705760/1‑150 (MQ=60)
                                                                                                                                              TGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCA  <  SRR19973070.3097945/150‑1 (MQ=60)
                                                                                                                                              TGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCA  >  SRR19973070.949072/1‑150 (MQ=60)
                                                                                                                                              TGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCA  >  SRR19973070.949232/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                 
ACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCA  >  CP009273/4287940‑4288231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑