Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 509,737 G→A 100% E97K (GAG→AAG)  cueR → copper‑responsive regulon transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273509,7370GA96.9% 635.1 / 8.0 194E97K (GAG→AAG) cueRcopper‑responsive regulon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base A (82/106);  total (85/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 195 total aligned reads displayed.

CTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATG  >  CP009273/509592‑509880
                                                                                                                                                 |                                                                                                                                               
cTTACGGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTgagaag                                                                                                                                               <  2:1534096/148‑1 (MQ=255)
     tGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGCCCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCa                                                                                                                                          <  1:483064/148‑1 (MQ=255)
        gcCAGGCACGGCTGGTGGGATTTAACCTGGAAGAGAGCGGCGAGCTGGTTAATGTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATc                                                                                                                                       <  1:627160/148‑1 (MQ=255)
         ccAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGATAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATcc                                                                                                                                      >  1:927641/1‑148 (MQ=255)
            ggCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATg                                                                                                                                   >  1:229125/1‑148 (MQ=255)
             gCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATgc                                                                                                                                  >  2:2813593/1‑148 (MQ=255)
              cACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATgcg                                                                                                                                 >  2:418863/1‑148 (MQ=255)
              cACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATgcg                                                                                                                                 >  2:2776551/1‑148 (MQ=255)
                cGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGcgcg                                                                                                                               >  1:2058430/1‑148 (MQ=255)
                   cAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGAcc                                                                                                                            <  2:551124/148‑1 (MQ=255)
                      gTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgc                                                                                                                         >  2:1503437/1‑148 (MQ=255)
                      gTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgc                                                                                                                         >  2:266295/1‑148 (MQ=255)
                         ggCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgctgc                                                                                                                      <  1:3176469/148‑1 (MQ=255)
                              tAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCa                                                                                                                 >  2:1853622/1‑148 (MQ=255)
                               aaCCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCAc                                                                                                                <  2:2599357/148‑1 (MQ=255)
                                 ccTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTg                                                                                                              <  2:2895988/148‑1 (MQ=255)
                                   tGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGc                                                                                                            >  2:3079050/1‑148 (MQ=255)
                                     gAAGAGAGCGGCGAGCTGGTGAATCTGTTTACCGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGa                                                                                                          <  2:2538653/148‑1 (MQ=255)
                                     gAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGa                                                                                                          <  2:187683/148‑1 (MQ=255)
                                     gAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGa                                                                                                          >  1:353181/1‑148 (MQ=255)
                                       agagagCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAt                                                                                                        >  2:729119/1‑148 (MQ=255)
                                        tcgcgcggcgAGCGGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCGGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGTGTTCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtg                                                                                                       <  1:2406796/144‑1 (MQ=255)
                                         agagCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtgc                                                                                                      <  1:3229885/148‑1 (MQ=255)
                                          gagCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGACtgcc                                                                                                     >  2:2757860/1‑148 (MQ=255)
                                          gagCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtgcc                                                                                                     >  2:1669924/1‑148 (MQ=255)
                                          gagCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtgcc                                                                                                     >  2:3429122/1‑148 (MQ=255)
                                            gcggcgAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtgcctg                                                                                                   <  1:3229262/148‑1 (MQ=255)
                                                cgAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCt                                                                                               >  2:1193157/1‑148 (MQ=255)
                                                cgAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCt                                                                                               >  2:1265766/1‑148 (MQ=255)
                                                    cTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCg                                                                                           >  1:2666567/1‑148 (MQ=255)
                                                      ggTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGAt                                                                                         >  1:1995485/1‑148 (MQ=255)
                                                      ggTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGAt                                                                                         >  2:1592703/1‑148 (MQ=255)
                                                      ggTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGAt                                                                                         >  1:2956059/1‑148 (MQ=255)
                                                      ggTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGAt                                                                                         >  1:2956414/1‑148 (MQ=255)
                                                      ggTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGAt                                                                                         <  1:2125583/148‑1 (MQ=255)
                                                       gTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATg                                                                                        <  1:1490925/148‑1 (MQ=255)
                                                          aatatttttAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGATGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACa                                                                                     <  1:2122236/142‑1 (MQ=255)
                                                          aaTCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACa                                                                                     <  1:2878758/148‑1 (MQ=255)
                                                          aaTCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACa                                                                                     <  1:2879026/148‑1 (MQ=255)
                                                           aTCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAg                                                                                    >  2:1746689/1‑148 (MQ=255)
                                                           aTCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAg                                                                                    >  2:637114/1‑148 (MQ=255)
                                                           aTCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAg                                                                                    >  2:2857897/1‑148 (MQ=255)
                                                             cTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAgcg                                                                                  <  1:1067733/148‑1 (MQ=255)
                                                             cTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAgcg                                                                                  <  2:53700/148‑1 (MQ=255)
                                                                 ttACCGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGa                                                                              <  2:335070/148‑1 (MQ=255)
                                                                 ttAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGa                                                                              >  1:2320439/1‑148 (MQ=255)
                                                                 ttAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGa                                                                              <  2:1484350/148‑1 (MQ=255)
                                                                  tAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGAc                                                                             <  2:2329712/148‑1 (MQ=255)
                                                                  tAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGAc                                                                             <  1:1846922/148‑1 (MQ=255)
                                                                     cGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGc                                                                          >  1:1913681/1‑148 (MQ=255)
                                                                        cccGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCg                                                                       <  2:2171576/148‑1 (MQ=255)
                                                                          cGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGAt                                                                     <  2:17367/148‑1 (MQ=255)
                                                                           gCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGAtt                                                                    <  1:1746689/148‑1 (MQ=255)
                                                                              gCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATc                                                                 >  2:1956871/1‑148 (MQ=255)
                                                                                 gCACAGCGCCGCCGTCAAACGGCGCACGCTGGAGAAGGGGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGTCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGaa                                                              <  1:625128/148‑1 (MQ=255)
                                                                                  cacaGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGaaa                                                             >  1:2578702/1‑148 (MQ=255)
                                                                                      gcgcCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAtct                                                         <  1:2891610/148‑1 (MQ=255)
                                                                                       cgcCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAtctc                                                        <  1:1035224/148‑1 (MQ=255)
                                                                                            aCGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCgg                                                   <  2:2927420/148‑1 (MQ=255)
                                                                                               tCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGgctg                                                >  2:3487027/1‑148 (MQ=255)
                                                                                                cAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGgctgc                                               >  2:3236613/1‑148 (MQ=255)
                                                                                                cAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGgctgc                                               <  1:3407813/148‑1 (MQ=255)
                                                                                                 aaaCGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGgctgct                                              >  1:110530/1‑148 (MQ=255)
                                                                                                   aCGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGt                                            <  2:13793/148‑1 (MQ=255)
                                                                                                   aCGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGt                                            >  1:2210497/1‑148 (MQ=255)
                                                                                                   aCGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGt                                            >  2:2384871/1‑148 (MQ=255)
                                                                                                        gcACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGtcatca                                       <  1:1555716/148‑1 (MQ=255)
                                                                                                         cACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTcatcat                                      <  1:3450474/148‑1 (MQ=255)
                                                                                                          aCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCatcatc                                     <  1:1075914/148‑1 (MQ=255)
                                                                                                          aCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCatcatc                                     <  2:2441577/148‑1 (MQ=255)
                                                                                                             cTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCggg                                  <  1:418863/148‑1 (MQ=255)
                                                                                                             cTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCggg                                  <  1:1592198/148‑1 (MQ=255)
                                                                                                               ggAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCa                                >  2:2461706/1‑148 (MQ=255)
                                                                                                                 agaAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAgg                              <  2:3195064/148‑1 (MQ=255)
                                                                                                                  gaAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAggg                             <  2:2054468/148‑1 (MQ=255)
                                                                                                                  gaAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAggg                             <  2:261863/148‑1 (MQ=255)
                                                                                                                  gaAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACGGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAggg                             <  1:2810251/148‑1 (MQ=255)
                                                                                                                    aGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTg                           <  1:1809465/148‑1 (MQ=255)
                                                                                                                    aGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTg                           >  1:1797332/1‑148 (MQ=255)
                                                                                                                    aGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTg                           <  2:353181/148‑1 (MQ=255)
                                                                                                                    aGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTg                           <  1:2644327/148‑1 (MQ=255)
                                                                                                                     ggTGGCTGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGa                          <  1:1834333/148‑1 (MQ=255)
                                                                                                                      gTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGAt                         >  1:2606025/1‑148 (MQ=255)
                                                                                                                      gTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGAt                         >  1:2033601/1‑148 (MQ=255)
                                                                                                                         tcGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTaa                      <  2:1333944/147‑1 (MQ=255)
                                                                                                                         gCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTaa                      >  2:1085455/1‑148 (MQ=255)
                                                                                                                           ggAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAaga                    <  2:968204/148‑1 (MQ=255)
                                                                                                                            gagaTCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagac                   >  1:2542192/1‑148 (MQ=255)
                                                                                                                               aTCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacgag                <  1:847317/148‑1 (MQ=255)
                                                                                                                                tCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacgaga               >  1:3263276/1‑148 (MQ=255)
                                                                                                                                tCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATAGGGCAGGGTGATTAagacgaga               >  2:3160333/1‑148 (MQ=255)
                                                                                                                                 cGAACGACACAGTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGAGTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacgagac              >  1:169210/1‑148 (MQ=255)
                                                                                                                                   aaCGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACgg            <  1:1352679/148‑1 (MQ=255)
                                                                                                                                   aaCGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACgg            >  1:2010809/1‑148 (MQ=255)
                                                                                                                                      gACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTc         >  1:2204660/1‑148 (MQ=255)
                                                                                                                                       acacATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCg        >  1:44699/1‑148 (MQ=255)
                                                                                                                                        cacaTTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCgg       >  1:1518002/1‑148 (MQ=255)
                                                                                                                                           aTTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGata    <  2:1816573/148‑1 (MQ=255)
                                                                                                                                            ttGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGatat   <  2:2868047/148‑1 (MQ=255)
                                                                                                                                             tGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATg  >  1:3050617/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATG  >  CP009273/509592‑509880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGG  >  CP009273/509591‑509885
                                                                                                                                                  |                                                                                                                                                    
CCTTACTGCGCCAGGCACGGCTGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGC                                                                                                                                                   <  SRR19973067.1926910/150‑1 (MQ=60)
 CTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCT                                                                                                                                                  >  SRR19973067.3215229/1‑150 (MQ=60)
  TTACTGCGCCTGGCCGGGCAGGTGGGCTTTAACGTGGAAGAGAGCGGGGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTG                                                                                                                                                 <  SRR19973067.2032894/150‑1 (MQ=60)
      TGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGCCCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAAT                                                                                                                                             <  SRR19973067.486387/150‑1 (MQ=60)
      TGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAAT                                                                                                                                             <  SRR19973067.486219/150‑1 (MQ=60)
        CGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGATAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCC                                                                                                                                           >  SRR19973067.933906/1‑150 (MQ=60)
        CGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGATAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCC                                                                                                                                           >  SRR19973067.933888/1‑150 (MQ=60)
         GCCAGGCACGGCTGGTGGGATTTAACCTGGAAGAGAGCGGCGAGCTGGTTAATGTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCA                                                                                                                                          <  SRR19973067.631333/150‑1 (MQ=60)
           CAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATG                                                                                                                                        >  SRR19973067.230767/1‑150 (MQ=60)
           CAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATG                                                                                                                                        >  SRR19973067.230774/1‑150 (MQ=60)
           CAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATG                                                                                                                                        >  SRR19973067.2921369/1‑150 (MQ=60)
            AGGCACGGCTGGGGGGCTTTAACCTGTAAGCGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGC                                                                                                                                       <  SRR19973067.340521/150‑1 (MQ=60)
               CACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCG                                                                                                                                    >  SRR19973067.2072149/1‑150 (MQ=60)
                    CAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAG                                                                                                                               <  SRR19973067.3525616/150‑1 (MQ=60)
                         GGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCT                                                                                                                          <  SRR19973067.752285/150‑1 (MQ=60)
                          GGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTG                                                                                                                         <  SRR19973067.3196571/150‑1 (MQ=60)
                                AACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTG                                                                                                                   >  SRR19973067.2269532/1‑150 (MQ=60)
                                 ACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGG                                                                                                                  >  SRR19973067.1828872/1‑150 (MQ=60)
                                    TGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGA                                                                                                               >  SRR19973067.355625/1‑150 (MQ=60)
                                      GAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAT                                                                                                             <  SRR19973067.2131030/150‑1 (MQ=60)
                                         tcgcGCGGCGAGCGGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCGGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGTGTTCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCC                                                                                                          <  SRR19973067.2422522/146‑1 (MQ=60)
                                         GAGCGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCC                                                                                                          <  SRR19973067.2422456/150‑1 (MQ=60)
                                          AGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCT                                                                                                         <  SRR19973067.3250158/150‑1 (MQ=60)
                                             GCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCC                                                                                                      <  SRR19973067.3249532/150‑1 (MQ=60)
                                               GGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCT                                                                                                    >  SRR19973067.3565305/1‑150 (MQ=60)
                                                  GAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGC                                                                                                 >  SRR19973067.1666113/1‑150 (MQ=60)
                                                   AGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCG                                                                                                >  SRR19973067.2683697/1‑150 (MQ=60)
                                                     CTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGAT                                                                                              >  SRR19973067.2974946/1‑150 (MQ=60)
                                                     CTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGAT                                                                                              >  SRR19973067.2974750/1‑150 (MQ=60)
                                                     CTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGAT                                                                                              >  SRR19973067.2975105/1‑150 (MQ=60)
                                                     CTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGAT                                                                                              >  SRR19973067.2008962/1‑150 (MQ=60)
                                                       GTTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACTTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGA                                                                                            <  SRR19973067.2712342/150‑1 (MQ=60)
                                                       GGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGA                                                                                            <  SRR19973067.2139769/150‑1 (MQ=60)
                                                        GTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGAC                                                                                           <  SRR19973067.1500788/150‑1 (MQ=60)
                                                           AATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGC                                                                                        <  SRR19973067.1428103/150‑1 (MQ=60)
                                                           AATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGC                                                                                        <  SRR19973067.2897179/150‑1 (MQ=60)
                                                           AATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGC                                                                                        <  SRR19973067.2897292/150‑1 (MQ=60)
                                                           AATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGC                                                                                        <  SRR19973067.2897447/150‑1 (MQ=60)
                                                           AATATTTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGATGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGC                                                                                        <  SRR19973067.2136399/150‑1 (MQ=60)
                                                              CTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCC                                                                                     <  SRR19973067.1075056/150‑1 (MQ=60)
                                                              CTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCC                                                                                     <  SRR19973067.1075108/150‑1 (MQ=60)
                                                               TGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCG                                                                                    <  SRR19973067.97058/150‑1 (MQ=60)
                                                                GTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGA                                                                                   >  SRR19973067.2335696/1‑150 (MQ=60)
                                                                   TAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTG                                                                                <  SRR19973067.1859442/150‑1 (MQ=60)
                                                                   TAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTG                                                                                <  SRR19973067.1859512/150‑1 (MQ=60)
                                                                    AACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGC                                                                               >  SRR19973067.1926741/1‑150 (MQ=60)
                                                                         CCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGAT                                                                          >  SRR19973067.444643/1‑150 (MQ=60)
                                                                            GCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTAT                                                                       <  SRR19973067.1758607/150‑1 (MQ=60)
                                                                             CAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATC                                                                      >  SRR19973067.2990172/1‑150 (MQ=60)
                                                                              AGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCG                                                                     >  SRR19973067.3281361/1‑150 (MQ=60)
                                                                                 GGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAA                                                                  >  SRR19973067.2595504/1‑150 (MQ=60)
                                                                                 GGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAA                                                                  >  SRR19973067.265220/1‑150 (MQ=60)
                                                                                  GCACAGCGCCGCCGTCAAACGGCGCACGCTGGAGAAGGGGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGTCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAA                                                                 <  SRR19973067.629258/150‑1 (MQ=60)
                                                                                  GCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGTCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAA                                                                 <  SRR19973067.629357/150‑1 (MQ=60)
                                                                                      AGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTC                                                             <  SRR19973067.2531715/150‑1 (MQ=60)
                                                                                       GCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCT                                                            <  SRR19973067.2908654/150‑1 (MQ=60)
                                                                                       GCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCT                                                            <  SRR19973067.2910086/150‑1 (MQ=60)
                                                                                        CGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTC                                                           <  SRR19973067.1042347/150‑1 (MQ=60)
                                                                                         GCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCC                                                          <  SRR19973067.2602441/150‑1 (MQ=60)
                                                                                                TCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCT                                                   >  SRR19973067.1921118/1‑150 (MQ=60)
                                                                                                TCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCT                                                   >  SRR19973067.111598/1‑150 (MQ=60)
                                                                                                 CAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTG                                                  <  SRR19973067.3429504/150‑1 (MQ=60)
                                                                                                  AAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGT                                                 >  SRR19973067.2225230/1‑150 (MQ=60)
                                                                                                       GCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCA                                            <  SRR19973067.28862/150‑1 (MQ=60)
                                                                                                        CGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCAT                                           >  SRR19973067.1536795/1‑150 (MQ=60)
                                                                                                         GCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATC                                          <  SRR19973067.1566315/150‑1 (MQ=60)
                                                                                                          CACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCG                                         <  SRR19973067.3472423/150‑1 (MQ=60)
                                                                                                           ACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGG                                        <  SRR19973067.1083339/150‑1 (MQ=60)
                                                                                                           ACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGG                                        <  SRR19973067.1083290/150‑1 (MQ=60)
                                                                                                            CGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGG                                       <  SRR19973067.268094/150‑1 (MQ=60)
                                                                                                              CTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCA                                     <  SRR19973067.1603110/150‑1 (MQ=60)
                                                                                                              CTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCA                                     <  SRR19973067.421773/150‑1 (MQ=60)
                                                                                                                 GAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGG                                  <  SRR19973067.2169040/150‑1 (MQ=60)
                                                                                                                   GAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG                                >  SRR19973067.1809390/1‑150 (MQ=60)
                                                                                                                   GAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG                                >  SRR19973067.1809416/1‑150 (MQ=60)
                                                                                                                   GAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACGGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG                                <  SRR19973067.2828364/150‑1 (MQ=60)
                                                                                                                     AGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGAT                              <  SRR19973067.2661405/150‑1 (MQ=60)
                                                                                                                     AGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGAT                              >  SRR19973067.2047224/1‑150 (MQ=60)
                                                                                                                     AGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGAT                              >  SRR19973067.3471841/1‑150 (MQ=60)
                                                                                                                     AGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGAT                              >  SRR19973067.2622959/1‑150 (MQ=60)
                                                                                                                     AGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGAT                              <  SRR19973067.1821695/150‑1 (MQ=60)
                                                                                                                     AGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGAT                              <  SRR19973067.1821694/150‑1 (MQ=60)
                                                                                                                     AGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGAT                              >  SRR19973067.2047225/1‑150 (MQ=60)
                                                                                                                      GGTGGCTGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATT                             <  SRR19973067.1846819/150‑1 (MQ=60)
                                                                                                                           CGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGAC                        <  SRR19973067.15949/150‑1 (MQ=60)
                                                                                                                           CGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGAC                        >  SRR19973067.2558936/1‑150 (MQ=60)
                                                                                                                           CGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGAC                        >  SRR19973067.2558835/1‑150 (MQ=60)
                                                                                                                               GATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGA                    >  SRR19973067.3283652/1‑150 (MQ=60)
                                                                                                                               GATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGA                    >  SRR19973067.1960473/1‑150 (MQ=60)
                                                                                                                                ATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGAC                   <  SRR19973067.853050/150‑1 (MQ=60)
                                                                                                                                ATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGAC                   >  SRR19973067.170808/1‑150 (MQ=60)
                                                                                                                                ATCGAACGACACAGTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGAGTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGAC                   >  SRR19973067.170589/1‑150 (MQ=60)
                                                                                                                                  CGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGG                 >  SRR19973067.2024341/1‑150 (MQ=60)
                                                                                                                                  CGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGG                 >  SRR19973067.2024310/1‑150 (MQ=60)
                                                                                                                                    AACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCT               <  SRR19973067.1361923/150‑1 (MQ=60)
                                                                                                                                     ACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTC              >  SRR19973067.2219362/1‑150 (MQ=60)
                                                                                                                                      CGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCG             >  SRR19973067.45310/1‑150 (MQ=60)
                                                                                                                                       GACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGG            >  SRR19973067.1528143/1‑150 (MQ=60)
                                                                                                                                       GACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGG            >  SRR19973067.1528146/1‑150 (MQ=60)
                                                                                                                                          ACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATA         <  SRR19973067.1347934/150‑1 (MQ=60)
                                                                                                                                            ATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATG       >  SRR19973067.3070051/1‑150 (MQ=60)
                                                                                                                                            ATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATG       >  SRR19973067.3070050/1‑150 (MQ=60)
                                                                                                                                            ATTGAGAAGCTGCAATCCAGGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATG       <  SRR19973067.2223865/150‑1 (MQ=60)
                                                                                                                                              TGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTA     <  SRR19973067.622651/150‑1 (MQ=60)
                                                                                                                                                AGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGG   <  SRR19973067.1094437/150‑1 (MQ=60)
                                                                                                                                                AGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGG   >  SRR19973067.895728/1‑150 (MQ=60)
                                                                                                                                                 GAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGG  >  SRR19973067.561955/1‑150 (MQ=60)
                                                                                                                                                 GAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGG  >  SRR19973067.2359885/1‑150 (MQ=60)
                                                                                                                                                 GAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGG  >  SRR19973067.2359829/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                    
CCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGG  >  CP009273/509591‑509885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑