Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 509,737 G→A 100% E97K (GAG→AAG)  cueR → copper‑responsive regulon transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273509,7370GA97.0% 555.2 / 5.6 170E97K (GAG→AAG) cueRcopper‑responsive regulon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (4/1);  new base A (72/92);  total (76/94)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 171 total aligned reads displayed.

CTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATAT  >  CP009273/509592‑509879
                                                                                                                                                 |                                                                                                                                              
cTTACTGCGCCAGGCACGGCTGGTGGGCTTTAACGTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTgagaag                                                                                                                                              <  1:2267094/148‑1 (MQ=255)
 ttACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGc                                                                                                                                             >  1:1123876/1‑148 (MQ=255)
 ttACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGc                                                                                                                                             <  1:3784733/148‑1 (MQ=255)
  tACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCt                                                                                                                                            >  1:2425410/1‑148 (MQ=255)
  tACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCt                                                                                                                                            >  1:2543666/1‑148 (MQ=255)
  tACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCt                                                                                                                                            >  2:1736554/1‑148 (MQ=255)
   aCTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTg                                                                                                                                           <  2:2364131/148‑1 (MQ=255)
      gcgcCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCaa                                                                                                                                        <  2:1212884/148‑1 (MQ=255)
        gcCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATc                                                                                                                                      <  1:3763357/148‑1 (MQ=255)
           aGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCAt                                                                                                                                   >  1:2473795/1‑148 (MQ=255)
             gCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATgc                                                                                                                                 >  2:531443/1‑148 (MQ=255)
                  gCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGAc                                                                                                                            >  1:2738712/1‑148 (MQ=255)
                  gCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGAc                                                                                                                            >  1:543561/1‑148 (MQ=255)
                  gCAGGTGGGCTTTAACCGGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGAc                                                                                                                            <  1:716802/148‑1 (MQ=255)
                      gTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgc                                                                                                                        <  2:379687/148‑1 (MQ=255)
                      gTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgc                                                                                                                        <  2:34717/148‑1 (MQ=255)
                         ggCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgctgc                                                                                                                     >  1:2636326/1‑148 (MQ=255)
                         ggCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgctgc                                                                                                                     >  1:2636297/1‑148 (MQ=255)
                             ttAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGc                                                                                                                 <  2:167650/148‑1 (MQ=255)
                             ttAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGc                                                                                                                 <  2:2848103/148‑1 (MQ=255)
                             ttAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGc                                                                                                                 >  2:1910841/1‑148 (MQ=255)
                                 ccTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTg                                                                                                             >  1:3253703/1‑148 (MQ=255)
                                 ccTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTg                                                                                                             >  1:3253691/1‑148 (MQ=255)
                                      aaGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGaa                                                                                                        <  2:413190/148‑1 (MQ=255)
                                       agagagCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAt                                                                                                       >  1:3627482/1‑148 (MQ=255)
                                       agagagCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAt                                                                                                       >  1:3021434/1‑148 (MQ=255)
                                        gagagCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtg                                                                                                      <  2:2993944/148‑1 (MQ=255)
                                        gagagCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtg                                                                                                      >  1:2465033/1‑148 (MQ=255)
                                         agagCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtgc                                                                                                     <  2:173826/148‑1 (MQ=255)
                                         agagCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtgc                                                                                                     <  2:1955193/148‑1 (MQ=255)
                                          gagCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtgcc                                                                                                    <  2:3418874/148‑1 (MQ=255)
                                           agCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtgcct                                                                                                   >  1:1461187/1‑148 (MQ=255)
                                           agCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtgcct                                                                                                   >  2:228592/1‑148 (MQ=255)
                                               gcgAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGccc                                                                                               <  2:1660749/148‑1 (MQ=255)
                                                  aGCTGTTTAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTgg                                                                                            <  1:671376/148‑1 (MQ=255)
                                                    cTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCg                                                                                          <  1:3134033/148‑1 (MQ=255)
                                                     tGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGa                                                                                         <  1:2039597/148‑1 (MQ=255)
                                                      ggTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGAt                                                                                        >  1:1654137/1‑148 (MQ=255)
                                                         gAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGAc                                                                                     <  1:3592401/148‑1 (MQ=255)
                                                          aaTCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACa                                                                                    <  2:1512334/148‑1 (MQ=255)
                                                          aaTCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACa                                                                                    <  2:1512393/148‑1 (MQ=255)
                                                          aaTCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACa                                                                                    <  2:473673/148‑1 (MQ=255)
                                                           aTCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAg                                                                                   >  2:2686389/1‑148 (MQ=255)
                                                           aTCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAg                                                                                   >  2:1292838/1‑148 (MQ=255)
                                                           aTCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAg                                                                                   <  1:991597/148‑1 (MQ=255)
                                                            tCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAgc                                                                                  >  2:2780317/1‑148 (MQ=255)
                                                            tCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAgc                                                                                  >  1:1341683/1‑148 (MQ=255)
                                                             cTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAgcg                                                                                 <  1:3419185/148‑1 (MQ=255)
                                                             cTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAgcg                                                                                 <  2:439761/148‑1 (MQ=255)
                                                                 ttAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGa                                                                             >  1:977699/1‑148 (MQ=255)
                                                                    aCGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGTACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTg                                                                          >  1:3157375/1‑148 (MQ=255)
                                                                     cGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGc                                                                         >  1:2977263/1‑148 (MQ=255)
                                                                     cGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGc                                                                         <  1:761079/148‑1 (MQ=255)
                                                                     cGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGc                                                                         >  2:1160414/1‑148 (MQ=255)
                                                                     cGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGc                                                                         <  2:702483/148‑1 (MQ=255)
                                                                         ccGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGa                                                                     <  1:289147/148‑1 (MQ=255)
                                                                          cGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGAt                                                                    <  2:1090685/148‑1 (MQ=255)
                                                                           gCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGAtt                                                                   <  2:3350014/148‑1 (MQ=255)
                                                                            cAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTa                                                                  >  2:1351895/1‑148 (MQ=255)
                                                                            cAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTa                                                                  >  2:1351844/1‑148 (MQ=255)
                                                                            cAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTa                                                                  >  1:1329982/1‑148 (MQ=255)
                                                                                  cacaGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGaaa                                                            <  2:2127414/148‑1 (MQ=255)
                                                                                    caGCGCCGACGTCAAACGTCGCACGCTGTAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCACTCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAt                                                          <  2:2562547/148‑1 (MQ=255)
                                                                                    caGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAt                                                          <  1:3191043/148‑1 (MQ=255)
                                                                                    caGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAt                                                          >  2:2018939/1‑148 (MQ=255)
                                                                                      gcgcCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAtct                                                        <  2:2473795/148‑1 (MQ=255)
                                                                                      gcgcCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAtct                                                        <  1:649285/148‑1 (MQ=255)
                                                                                       cgcCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAtctc                                                       <  2:3253703/148‑1 (MQ=255)
                                                                                       cgcCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAtctc                                                       <  2:3253691/148‑1 (MQ=255)
                                                                                        gcCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAtctct                                                      <  2:1538159/148‑1 (MQ=255)
                                                                                            aCGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCgg                                                  <  2:1740970/148‑1 (MQ=255)
                                                                                            aCGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCgg                                                  <  2:2166830/148‑1 (MQ=255)
                                                                                                cAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGgctgc                                              <  2:3510264/148‑1 (MQ=255)
                                                                                                   aCGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGt                                           >  2:1475410/1‑148 (MQ=255)
                                                                                                      gcgcACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGtcat                                        >  1:2842095/1‑148 (MQ=255)
                                                                                                           cGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCg                                   <  2:3009254/148‑1 (MQ=255)
                                                                                                              tGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGc                                <  1:1147075/148‑1 (MQ=255)
                                                                                                                gagaAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAg                              >  2:1616220/1‑148 (MQ=255)
                                                                                                                 agaAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAgg                             <  1:1451275/148‑1 (MQ=255)
                                                                                                                   aaGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGt                           >  1:104437/1‑148 (MQ=255)
                                                                                                                    aGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTg                          <  2:2921046/148‑1 (MQ=255)
                                                                                                                     ggTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGa                         >  2:1877671/1‑148 (MQ=255)
                                                                                                                     ggTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGa                         >  2:2417323/1‑148 (MQ=255)
                                                                                                                     ggTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGa                         <  1:1468361/148‑1 (MQ=255)
                                                                                                                          cGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAag                    <  1:3111057/148‑1 (MQ=255)
                                                                                                                             agaTCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacg                 <  1:1035964/148‑1 (MQ=255)
                                                                                                                             agaTCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacg                 <  1:713683/148‑1 (MQ=255)
                                                                                                                               aTCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacgag               <  2:1123876/148‑1 (MQ=255)
                                                                                                                               aTCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacgag               >  1:2116245/1‑148 (MQ=255)
                                                                                                                               aTCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacgag               <  1:828285/148‑1 (MQ=255)
                                                                                                                                    aCGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACNGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGc          >  1:1935839/1‑148 (MQ=255)
                                                                                                                                    aCGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGc          >  1:1770762/1‑148 (MQ=255)
                                                                                                                                    aCGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGc          >  1:1770648/1‑148 (MQ=255)
                                                                                                                                       acacATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCg       >  2:3758159/1‑148 (MQ=255)
                                                                                                                                        cacaTTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCgg      >  2:2231797/1‑148 (MQ=255)
                                                                                                                                        cacaTTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCgg      >  1:437438/1‑148 (MQ=255)
                                                                                                                                          caTTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGat    <  1:519805/148‑1 (MQ=255)
                                                                                                                                          caTTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGat    <  2:2374072/148‑1 (MQ=255)
                                                                                                                                            ttGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGatat  <  2:370036/148‑1 (MQ=255)
                                                                                                                                            ttGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGatat  >  1:68941/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATAT  >  CP009273/509592‑509879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGG  >  CP009273/509588‑509884
                                                                                                                                                     |                                                                                                                                                   
TGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGA                                                                                                                                                     >  SRR19973069.3054264/1‑150 (MQ=60)
TGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGA                                                                                                                                                     >  SRR19973069.3054268/1‑150 (MQ=60)
TGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGA                                                                                                                                                     >  SRR19973069.3054275/1‑150 (MQ=60)
 GACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAA                                                                                                                                                    >  SRR19973069.2090281/1‑150 (MQ=60)
   CCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGC                                                                                                                                                  >  SRR19973069.1131714/1‑150 (MQ=60)
    CTTACTGCGCCAGGCACGGCTGGTGGGCTTTAACGTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCT                                                                                                                                                 <  SRR19973069.2282452/150‑1 (MQ=60)
    CTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCT                                                                                                                                                 >  SRR19973069.2441799/1‑150 (MQ=60)
    CTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCT                                                                                                                                                 >  SRR19973069.1485702/1‑150 (MQ=60)
    CTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCT                                                                                                                                                 >  SRR19973069.2441779/1‑150 (MQ=60)
    CTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCT                                                                                                                                                 >  SRR19973069.2560492/1‑150 (MQ=60)
     TTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTG                                                                                                                                                <  SRR19973069.3809291/150‑1 (MQ=60)
         TGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAAT                                                                                                                                            <  SRR19973069.3789871/150‑1 (MQ=60)
            GCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCA                                                                                                                                         <  SRR19973069.3787441/150‑1 (MQ=60)
            GCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCA                                                                                                                                         <  SRR19973069.3787822/150‑1 (MQ=60)
             CCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCAT                                                                                                                                        >  SRR19973069.2490384/1‑150 (MQ=60)
                    CGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGAC                                                                                                                                 >  SRR19973069.547377/1‑150 (MQ=60)
                    CGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGAC                                                                                                                                 >  SRR19973069.547241/1‑150 (MQ=60)
                    CGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGAC                                                                                                                                 >  SRR19973069.2757029/1‑150 (MQ=60)
                      GCAGGTGGGCTTTAACCGGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCA                                                                                                                               <  SRR19973069.721850/150‑1 (MQ=60)
                           TGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGC                                                                                                                          >  SRR19973069.725549/1‑150 (MQ=60)
                           TGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGC                                                                                                                          >  SRR19973069.2653954/1‑150 (MQ=60)
                           TGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGC                                                                                                                          >  SRR19973069.2653983/1‑150 (MQ=60)
                             GGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTG                                                                                                                        <  SRR19973069.3503260/150‑1 (MQ=60)
                                   AACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTG                                                                                                                  >  SRR19973069.3275011/1‑150 (MQ=60)
                                   AACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTG                                                                                                                  >  SRR19973069.3274999/1‑150 (MQ=60)
                                     CCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGC                                                                                                                >  SRR19973069.2854370/1‑150 (MQ=60)
                                      CTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCG                                                                                                               >  SRR19973069.3323572/1‑150 (MQ=60)
                                      CTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCG                                                                                                               <  SRR19973069.2311866/150‑1 (MQ=60)
                                         GAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAT                                                                                                            >  SRR19973069.3651194/1‑150 (MQ=60)
                                         GAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAT                                                                                                            >  SRR19973069.3041473/1‑150 (MQ=60)
                                          AAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATG                                                                                                           >  SRR19973069.2481581/1‑150 (MQ=60)
                                           AGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGC                                                                                                          >  SRR19973069.2004276/1‑150 (MQ=60)
                                             AGAGCGGCGAGCTGGTGAATCTTTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCT                                                                                                        <  SRR19973069.3247574/150‑1 (MQ=60)
                                             AGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCT                                                                                                        >  SRR19973069.1471381/1‑150 (MQ=60)
                                             AGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCT                                                                                                        >  SRR19973069.771658/1‑150 (MQ=60)
                                                     GAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGC                                                                                                >  SRR19973069.2142050/1‑150 (MQ=60)
                                                      AGCTGTTTAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCG                                                                                               <  SRR19973069.675994/150‑1 (MQ=60)
                                                        CTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGAT                                                                                             <  SRR19973069.3154577/150‑1 (MQ=60)
                                                        CTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGAT                                                                                             >  SRR19973069.1665580/1‑150 (MQ=60)
                                                         TGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATG                                                                                            <  SRR19973069.2053761/150‑1 (MQ=60)
                                                             GAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAG                                                                                        <  SRR19973069.3615838/150‑1 (MQ=60)
                                                              AATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGC                                                                                       >  SRR19973069.1350956/1‑150 (MQ=60)
                                                               ATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCG                                                                                      <  SRR19973069.998333/150‑1 (MQ=60)
                                                                 CTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCC                                                                                    <  SRR19973069.3441446/150‑1 (MQ=60)
                                                                   GTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGA                                                                                  >  SRR19973069.984283/1‑150 (MQ=60)
                                                                      TAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGTACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTG                                                                               >  SRR19973069.3177987/1‑150 (MQ=60)
                                                                      TAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGTACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTG                                                                               >  SRR19973069.3177453/1‑150 (MQ=60)
                                                                       AACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGC                                                                              >  SRR19973069.2997033/1‑150 (MQ=60)
                                                                       AACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGC                                                                              >  SRR19973069.2997395/1‑150 (MQ=60)
                                                                        ACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCC                                                                             <  SRR19973069.1729655/150‑1 (MQ=60)
                                                                         CGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCC                                                                            <  SRR19973069.766456/150‑1 (MQ=60)
                                                                             CCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATT                                                                        <  SRR19973069.291143/150‑1 (MQ=60)
                                                                             CCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATT                                                                        <  SRR19973069.291114/150‑1 (MQ=60)
                                                                              CGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTA                                                                       >  SRR19973069.1339935/1‑150 (MQ=60)
                                                                              CGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTA                                                                       >  SRR19973069.1339133/1‑150 (MQ=60)
                                                                                 AGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCG                                                                    <  SRR19973069.1071375/150‑1 (MQ=60)
                                                                                        CAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCT                                                             <  SRR19973069.3211762/150‑1 (MQ=60)
                                                                                        CAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCANGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCT                                                             <  SRR19973069.1945869/150‑1 (MQ=60)
                                                                                          GCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCT                                                           <  SRR19973069.653588/150‑1 (MQ=60)
                                                                                            GCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCC                                                         <  SRR19973069.611591/150‑1 (MQ=60)
                                                                                                  GTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGC                                                   >  SRR19973069.684825/1‑150 (MQ=60)
                                                                                                  GTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGC                                                   >  SRR19973069.2614909/1‑150 (MQ=60)
                                                                                                   TCAACCGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCT                                                  <  SRR19973069.2689165/150‑1 (MQ=60)
                                                                                                        CGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCAT                                             >  SRR19973069.2860843/1‑150 (MQ=60)
                                                                                                        CGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCAT                                             >  SRR19973069.932448/1‑150 (MQ=60)
                                                                                                             CACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCG                                        <  SRR19973069.3191026/150‑1 (MQ=60)
                                                                                                                  TGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAG                                   <  SRR19973069.1154745/150‑1 (MQ=60)
                                                                                                                  TGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAG                                   <  SRR19973069.1155082/150‑1 (MQ=60)
                                                                                                                     AGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGT                                >  SRR19973069.105469/1‑150 (MQ=60)
                                                                                                                     AGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGT                                <  SRR19973069.1461406/150‑1 (MQ=60)
                                                                                                                      GAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG                               <  SRR19973069.3054417/150‑1 (MQ=60)
                                                                                                                         GGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATT                            <  SRR19973069.1478588/150‑1 (MQ=60)
                                                                                                                         GGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATT                            <  SRR19973069.1478602/150‑1 (MQ=60)
                                                                                                                              CGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGAC                       <  SRR19973069.3131514/150‑1 (MQ=60)
                                                                                                                                 AGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAG                    <  SRR19973069.1043157/150‑1 (MQ=60)
                                                                                                                                 AGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAG                    <  SRR19973069.718709/150‑1 (MQ=60)
                                                                                                                                 AGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAG                    >  SRR19973069.2130833/1‑150 (MQ=60)
                                                                                                                                   ATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGAC                  <  SRR19973069.834117/150‑1 (MQ=60)
                                                                                                                                   ATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGAC                  <  SRR19973069.443802/150‑1 (MQ=60)
                                                                                                                                      GAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACNGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGC               >  SRR19973069.1949140/1‑150 (MQ=60)
                                                                                                                                      GAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGC               >  SRR19973069.2521/1‑150 (MQ=60)
                                                                                                                                      GAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGC               >  SRR19973069.1783120/1‑150 (MQ=60)
                                                                                                                                      GAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGC               >  SRR19973069.1783236/1‑150 (MQ=60)
                                                                                                                                          GACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGG           >  SRR19973069.440641/1‑150 (MQ=60)
                                                                                                                                              CATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATAT       >  SRR19973069.1423565/1‑150 (MQ=60)
                                                                                                                                              CATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATAT       >  SRR19973069.69734/1‑150 (MQ=60)
                                                                                                                                              CATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATAT       >  SRR19973069.70298/1‑150 (MQ=60)
                                                                                                                                              CATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATAT       <  SRR19973069.523496/150‑1 (MQ=60)
                                                                                                                                                   AGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGG  <  SRR19973069.2182517/150‑1 (MQ=60)
                                                                                                                                                   AGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGG  <  SRR19973069.1542228/150‑1 (MQ=60)
                                                                                                                                                   AGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGG  <  SRR19973069.290713/150‑1 (MQ=60)
                                                                                                                                                   AGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGG  >  SRR19973069.2995287/1‑150 (MQ=60)
                                                                                                                                                   AGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGG  <  SRR19973069.3453248/150‑1 (MQ=60)
                                                                                                                                                   AGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGACCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGG  >  SRR19973069.2994426/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
TGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGG  >  CP009273/509588‑509884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑