Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 509,737 G→A 100% E97K (GAG→AAG)  cueR → copper‑responsive regulon transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273509,7370GA95.7% 579.9 / 13.5 184E97K (GAG→AAG) cueRcopper‑responsive regulon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (3/5);  new base A (83/93);  total (86/98)
Fisher's exact test for biased strand distribution p-value = 7.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 185 total aligned reads displayed.

CTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATG  >  CP009273/509592‑509880
                                                                                                                                                 |                                                                                                                                               
cTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTgaggag                                                                                                                                               >  1:1535953/1‑148 (MQ=255)
 ttACTGCGCCCGGCACGGCTGGTGGGATTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGc                                                                                                                                              <  1:1294732/148‑1 (MQ=255)
 ttACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGc                                                                                                                                              >  1:2501558/1‑148 (MQ=255)
 ttACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGc                                                                                                                                              <  1:819267/148‑1 (MQ=255)
  tACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCt                                                                                                                                             >  2:319491/1‑148 (MQ=255)
    cTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGc                                                                                                                                           >  2:1265273/1‑148 (MQ=255)
       cgcCAGGCACGGCAGGTGGGCTTTACCCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAAt                                                                                                                                        <  1:2772386/148‑1 (MQ=255)
       cgcCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAAt                                                                                                                                        <  2:1443462/148‑1 (MQ=255)
         ccAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATcc                                                                                                                                      <  1:764744/148‑1 (MQ=255)
            ggCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATg                                                                                                                                   <  2:1608054/148‑1 (MQ=255)
             gCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATgc                                                                                                                                  >  2:1132486/1‑148 (MQ=255)
             gCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATgc                                                                                                                                  >  2:357009/1‑148 (MQ=255)
             gCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATgc                                                                                                                                  >  2:357269/1‑148 (MQ=255)
              cACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATgcg                                                                                                                                 >  2:1687768/1‑148 (MQ=255)
                 ggCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGa                                                                                                                              <  2:1548129/148‑1 (MQ=255)
                 ggCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGa                                                                                                                              >  2:1561377/1‑148 (MQ=255)
                  gCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGAc                                                                                                                             >  1:288343/1‑148 (MQ=255)
                   cAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGAcc                                                                                                                            <  2:88297/148‑1 (MQ=255)
                        gggCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgctg                                                                                                                       <  2:3527389/148‑1 (MQ=255)
                        gggCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgctg                                                                                                                       <  2:3068656/148‑1 (MQ=255)
                         ggCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgctgc                                                                                                                      <  1:2497631/148‑1 (MQ=255)
                         ggCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgctgc                                                                                                                      <  1:2497662/148‑1 (MQ=255)
                         ggCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgctgc                                                                                                                      >  2:349704/1‑148 (MQ=255)
                         ggCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgctgc                                                                                                                      <  1:2778510/148‑1 (MQ=255)
                          gCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAgctgct                                                                                                                     <  2:1928341/148‑1 (MQ=255)
                           cTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGctgctg                                                                                                                    >  2:3336284/1‑148 (MQ=255)
                           cTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGACAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGctgctg                                                                                                                    >  2:2058542/1‑148 (MQ=255)
                                   tGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGc                                                                                                            >  1:841636/1‑148 (MQ=255)
                                    ggAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCg                                                                                                           >  2:1270687/1‑148 (MQ=255)
                                     gAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGa                                                                                                          <  2:340870/148‑1 (MQ=255)
                                     gAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGa                                                                                                          <  2:341010/148‑1 (MQ=255)
                                      aaGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGaa                                                                                                         >  1:3392741/1‑148 (MQ=255)
                                       agagagCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAt                                                                                                        >  1:863851/1‑148 (MQ=255)
                                        gagagCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtg                                                                                                       <  2:1502406/148‑1 (MQ=255)
                                         agagCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtgc                                                                                                      >  2:3321101/1‑148 (MQ=255)
                                         agagCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtgc                                                                                                      <  2:2587000/148‑1 (MQ=255)
                                             cggcgAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtgcctgc                                                                                                  >  2:982755/1‑148 (MQ=255)
                                                      ggTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGAt                                                                                         <  2:2197171/148‑1 (MQ=255)
                                                         gAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGAc                                                                                      <  1:2348431/148‑1 (MQ=255)
                                                           aTCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAg                                                                                    <  1:2654718/148‑1 (MQ=255)
                                                           aTCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAg                                                                                    <  1:579768/148‑1 (MQ=255)
                                                           aTCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAg                                                                                    >  1:2997825/1‑148 (MQ=255)
                                                             cTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAgcg                                                                                  <  1:2388274/148‑1 (MQ=255)
                                                             cTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAgcg                                                                                  >  1:2656911/1‑148 (MQ=255)
                                                              tGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAgcgc                                                                                 <  2:1848768/148‑1 (MQ=255)
                                                               gTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGcc                                                                                <  1:284018/148‑1 (MQ=255)
                                                                  tAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGAc                                                                             <  1:2304882/148‑1 (MQ=255)
                                                                   aaCGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACt                                                                            >  1:2238077/1‑148 (MQ=255)
                                                                     cGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGc                                                                          >  2:1420464/1‑148 (MQ=255)
                                                                      gACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGcc                                                                         <  2:2751336/148‑1 (MQ=255)
                                                                         ccGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGa                                                                      <  1:3278648/148‑1 (MQ=255)
                                                                          cGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGAt                                                                     <  1:458632/148‑1 (MQ=255)
                                                                              gCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATc                                                                 >  1:439290/1‑148 (MQ=255)
                                                                               cGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCg                                                                >  2:1313562/1‑148 (MQ=255)
                                                                               cGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCg                                                                >  2:716629/1‑148 (MQ=255)
                                                                               cGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCg                                                                >  2:2504983/1‑148 (MQ=255)
                                                                                   acaGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGaaaa                                                            <  2:3512908/148‑1 (MQ=255)
                                                                                    caGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAt                                                           <  2:2115175/148‑1 (MQ=255)
                                                                                       cgcCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAtctc                                                        >  1:2951279/1‑148 (MQ=255)
                                                                                        gcCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAtctct                                                       <  1:1132486/148‑1 (MQ=255)
                                                                                        gcCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAtctct                                                       <  2:455750/148‑1 (MQ=255)
                                                                                         ccGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGACCGACACATTGAGAAGCTGCAATCCTGGCGCGACCTGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAtctctc                                                      <  2:1975800/148‑1 (MQ=255)
                                                                                               tCAACCGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGgctg                                                <  1:311007/148‑1 (MQ=255)
                                                                                               tCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGgctg                                                <  2:2854278/148‑1 (MQ=255)
                                                                                                cAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGgctgc                                               <  2:1801002/148‑1 (MQ=255)
                                                                                                   aCGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGt                                            >  1:367585/1‑148 (MQ=255)
                                                                                                   aCGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGt                                            >  1:2460108/1‑148 (MQ=255)
                                                                                                   aCGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGt                                            >  1:2459421/1‑148 (MQ=255)
                                                                                                     ggCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGtca                                          <  1:1185422/148‑1 (MQ=255)
                                                                                                         cACGCTGGAGACGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTcatcat                                      <  1:2047881/148‑1 (MQ=255)
                                                                                                           cGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCg                                    <  1:637135/148‑1 (MQ=255)
                                                                                                                 agaAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAgg                              <  2:1625590/148‑1 (MQ=255)
                                                                                                                  gaAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAggg                             <  2:2333515/148‑1 (MQ=255)
                                                                                                                   aaGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGt                            >  2:52852/1‑148 (MQ=255)
                                                                                                                    aGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTg                           <  2:1365188/148‑1 (MQ=255)
                                                                                                                      gTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGAt                         <  1:3521893/148‑1 (MQ=255)
                                                                                                                         gCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTaa                      >  1:1794405/1‑148 (MQ=255)
                                                                                                                          cGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAag                     <  1:1265273/148‑1 (MQ=255)
                                                                                                                           ggAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAaga                    <  1:326298/148‑1 (MQ=255)
                                                                                                                             agaTCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacg                  >  1:3110950/1‑148 (MQ=255)
                                                                                                                               aTCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacgag                >  1:1258082/1‑148 (MQ=255)
                                                                                                                               aTCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacgag                <  1:3477771/148‑1 (MQ=255)
                                                                                                                               aTCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacgag                >  2:2348962/1‑148 (MQ=255)
                                                                                                                                tCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacgaga               <  2:2891463/148‑1 (MQ=255)
                                                                                                                                tCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacgaga               >  1:2198560/1‑148 (MQ=255)
                                                                                                                                tCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacgaga               >  2:2118912/1‑148 (MQ=255)
                                                                                                                                tCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacgaga               <  1:252791/148‑1 (MQ=255)
                                                                                                                                tCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacgaga               <  2:2658469/148‑1 (MQ=255)
                                                                                                                                 cGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTTCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacgagac              >  1:107687/1‑148 (MQ=255)
                                                                                                                                 cGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAagacgagac              >  1:544282/1‑148 (MQ=255)
                                                                                                                                   aaCGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACgg            <  1:28484/148‑1 (MQ=255)
                                                                                                                                    aCGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGc           >  1:568142/1‑148 (MQ=255)
                                                                                                                                     cGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCt          >  1:1314920/1‑148 (MQ=255)
                                                                                                                                     cGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCt          >  2:1486904/1‑148 (MQ=255)
                                                                                                                                     cGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCt          <  1:1069639/148‑1 (MQ=255)
                                                                                                                                           aTTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGata    <  1:1024768/148‑1 (MQ=255)
                                                                                                                                           aTTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGata    <  1:1024878/148‑1 (MQ=255)
                                                                                                                                           aTTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGata    <  2:1113913/148‑1 (MQ=255)
                                                                                                                                             tGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATg  >  1:3494270/1‑148 (MQ=255)
                                                                                                                                             tGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATg  >  1:706708/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATG  >  CP009273/509592‑509880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGT  >  CP009273/509588‑509886
                                                                                                                                                     |                                                                                                                                                     
TGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGA                                                                                                                                                       >  SRR19973066.1924950/1‑150 (MQ=60)
 GACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAA                                                                                                                                                      <  SRR19973066.1279203/150‑1 (MQ=60)
  ACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAG                                                                                                                                                     >  SRR19973066.1546902/1‑150 (MQ=60)
   CCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGC                                                                                                                                                    >  SRR19973066.2518599/1‑150 (MQ=60)
   CCTTACTGCGCCAGGCACGGCAGGTGGGATTTAACCTGGAAGAGCGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGC                                                                                                                                                    <  SRR19973066.3462265/150‑1 (MQ=60)
     TTACTGCGCCCGGCACGGCTGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTG                                                                                                                                                  <  SRR19973066.1304065/150‑1 (MQ=60)
     TTACTGCGCCCGGCACGGCTGGTGGGATTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTG                                                                                                                                                  <  SRR19973066.1304112/150‑1 (MQ=60)
     TTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTG                                                                                                                                                  <  SRR19973066.825256/150‑1 (MQ=60)
           CGCCAGGCACGGCAGGTGGGCTTTACCCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCC                                                                                                                                            <  SRR19973066.2791053/150‑1 (MQ=60)
             CCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCAT                                                                                                                                          <  SRR19973066.770410/150‑1 (MQ=60)
             CCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCAT                                                                                                                                          <  SRR19973066.769308/150‑1 (MQ=60)
              CAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATG                                                                                                                                         >  SRR19973066.2349432/1‑150 (MQ=60)
                  CACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCG                                                                                                                                     >  SRR19973066.342098/1‑150 (MQ=60)
                  CACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCG                                                                                                                                     >  SRR19973066.341559/1‑150 (MQ=60)
                    CGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGAC                                                                                                                                   >  SRR19973066.290414/1‑150 (MQ=60)
                    CGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGAC                                                                                                                                   >  SRR19973066.286833/1‑150 (MQ=60)
                         GGTGGGCTTTAACCTGGACGCGTGCGGCGAGGTGGTGAATATTTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCT                                                                                                                              <  SRR19973066.398250/150‑1 (MQ=60)
                             GGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTG                                                                                                                          <  SRR19973066.2514683/150‑1 (MQ=60)
                             GGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTG                                                                                                                          <  SRR19973066.2514652/150‑1 (MQ=60)
                             GGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTG                                                                                                                          <  SRR19973066.2797216/150‑1 (MQ=60)
                             GGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTG                                                                                                                          <  SRR19973066.2797388/150‑1 (MQ=60)
                             GGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTG                                                                                                                          <  SRR19973066.2514398/150‑1 (MQ=60)
                               CTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGC                                                                                                                        <  SRR19973066.2422267/150‑1 (MQ=60)
                                  TAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACT                                                                                                                     >  SRR19973066.813933/1‑150 (MQ=60)
                                    ACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGATGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGG                                                                                                                   >  SRR19973066.1320556/1‑150 (MQ=60)
                                     CCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGC                                                                                                                  >  SRR19973066.846724/1‑150 (MQ=60)
                                     CCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGC                                                                                                                  >  SRR19973066.847750/1‑150 (MQ=60)
                                        GGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAA                                                                                                               >  SRR19973066.3415380/1‑150 (MQ=60)
                                         GAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAT                                                                                                              >  SRR19973066.870058/1‑150 (MQ=60)
                                         GAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAT                                                                                                              >  SRR19973066.869896/1‑150 (MQ=60)
                                            GAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCC                                                                                                           >  SRR19973066.2676293/1‑150 (MQ=60)
                                               AGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGC                                                                                                        >  SRR19973066.2529724/1‑150 (MQ=60)
                                                             GAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAG                                                                                          <  SRR19973066.2364396/150‑1 (MQ=60)
                                                             GAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAG                                                                                          >  SRR19973066.3017719/1‑150 (MQ=60)
                                                             GAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAG                                                                                          <  SRR19973066.3504916/150‑1 (MQ=60)
                                                               ATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCG                                                                                        >  SRR19973066.2674727/1‑150 (MQ=60)
                                                               ATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCG                                                                                        >  SRR19973066.2674711/1‑150 (MQ=60)
                                                               ATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCG                                                                                        <  SRR19973066.2672525/150‑1 (MQ=60)
                                                               ATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCG                                                                                        <  SRR19973066.583836/150‑1 (MQ=60)
                                                                 CTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCC                                                                                      <  SRR19973066.2404359/150‑1 (MQ=60)
                                                                   GTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGA                                                                                    <  SRR19973066.286071/150‑1 (MQ=60)
                                                                     TTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACT                                                                                  >  SRR19973066.2253511/1‑150 (MQ=60)
                                                                      TAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTG                                                                                 <  SRR19973066.2320668/150‑1 (MQ=60)
                                                                          GACCCGCAGCGGCACCGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCG                                                                             <  SRR19973066.352433/150‑1 (MQ=60)
                                                                           ACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGA                                                                            <  SRR19973066.2949436/150‑1 (MQ=60)
                                                                           ACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAAGCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGA                                                                            <  SRR19973066.1158615/150‑1 (MQ=60)
                                                                            CCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGAT                                                                           <  SRR19973066.2273259/150‑1 (MQ=60)
                                                                             CCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATT                                                                          <  SRR19973066.3300180/150‑1 (MQ=60)
                                                                              CGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTA                                                                         <  SRR19973066.462148/150‑1 (MQ=60)
                                                                               GCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTAT                                                                        <  SRR19973066.2058695/150‑1 (MQ=60)
                                                                                CAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATC                                                                       >  SRR19973066.442688/1‑150 (MQ=60)
                                                                                   CGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAA                                                                    >  SRR19973066.2992695/1‑150 (MQ=60)
                                                                                   CGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAA                                                                    >  SRR19973066.917056/1‑150 (MQ=60)
                                                                                    GGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAA                                                                   >  SRR19973066.570007/1‑150 (MQ=60)
                                                                                      CACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAT                                                                 <  SRR19973066.2210099/150‑1 (MQ=60)
                                                                                      CACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAT                                                                 <  SRR19973066.2517500/150‑1 (MQ=60)
                                                                                         AGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTC                                                              >  SRR19973066.1519746/1‑150 (MQ=60)
                                                                                         AGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTC                                                              >  SRR19973066.2970860/1‑150 (MQ=60)
                                                                                          GCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCT                                                             <  SRR19973066.2671124/150‑1 (MQ=60)
                                                                                            GCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCC                                                           <  SRR19973066.1140607/150‑1 (MQ=60)
                                                                                             CCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCG                                                          <  SRR19973066.236758/150‑1 (MQ=60)
                                                                                              CGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGG                                                         >  SRR19973066.1084912/1‑150 (MQ=60)
                                                                                                   TCAACCGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCT                                                    <  SRR19973066.313291/150‑1 (MQ=60)
                                                                                                     AAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGT                                                  >  SRR19973066.370471/1‑150 (MQ=60)
                                                                                                     AAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGT                                                  >  SRR19973066.2476872/1‑150 (MQ=60)
                                                                                                     AAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGT                                                  >  SRR19973066.2476183/1‑150 (MQ=60)
                                                                                                     AAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGT                                                  >  SRR19973066.2476875/1‑150 (MQ=60)
                                                                                                         GGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATC                                              <  SRR19973066.1193744/150‑1 (MQ=60)
                                                                                                           CGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCTATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCAT                                            >  SRR19973066.2900362/1‑150 (MQ=60)
                                                                                                             CACGCTGGAGACGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCG                                          <  SRR19973066.2062119/150‑1 (MQ=60)
                                                                                                               CGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGG                                        <  SRR19973066.641728/150‑1 (MQ=60)
                                                                                                                          GTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTA                             <  SRR19973066.3545340/150‑1 (MQ=60)
                                                                                                                           TGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAA                            >  SRR19973066.3296660/1‑150 (MQ=60)
                                                                                                                           TGGCGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAA                            >  SRR19973066.1807172/1‑150 (MQ=60)
                                                                                                                              CGGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGAC                         <  SRR19973066.1274410/150‑1 (MQ=60)
                                                                                                                               GGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACG                        <  SRR19973066.328778/150‑1 (MQ=60)
                                                                                                                               GGAGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACG                        >  SRR19973066.3131731/1‑150 (MQ=60)
                                                                                                                                 AGATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAG                      >  SRR19973066.1267151/1‑150 (MQ=60)
                                                                                                                                  GATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGA                     >  SRR19973066.2213802/1‑150 (MQ=60)
                                                                                                                                   ATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTTCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGAC                    >  SRR19973066.108796/1‑150 (MQ=60)
                                                                                                                                   ATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTTCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGAC                    >  SRR19973066.3029940/1‑150 (MQ=60)
                                                                                                                                   ATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGAC                    <  SRR19973066.3500982/150‑1 (MQ=60)
                                                                                                                                   ATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGAC                    >  SRR19973066.548214/1‑150 (MQ=60)
                                                                                                                                   ATCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGAC                    >  SRR19973066.548200/1‑150 (MQ=60)
                                                                                                                                    TCGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACG                   <  SRR19973066.254720/150‑1 (MQ=60)
                                                                                                                                     CGAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGTCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGG                  >  SRR19973066.3239311/1‑150 (MQ=60)
                                                                                                                                      GAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGC                 >  SRR19973066.572162/1‑150 (MQ=60)
                                                                                                                                      GAACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGC                 >  SRR19973066.573372/1‑150 (MQ=60)
                                                                                                                                       AACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCT                >  SRR19973066.1324454/1‑150 (MQ=60)
                                                                                                                                       AACGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCT                <  SRR19973066.28882/150‑1 (MQ=60)
                                                                                                                                         CGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCG              <  SRR19973066.1077483/150‑1 (MQ=60)
                                                                                                                                         CGACACATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCG              <  SRR19973066.1077492/150‑1 (MQ=60)
                                                                                                                                              CATTGATAAGCTGCAATCCATGCGCGACGAGCTGGTGGCAGGGGCGAATGCCTGCCCTGGCTATGACTGCGCCGACTGCCCGTTTATCGAAAATCTCTCGGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATAT         <  SRR19973066.46239/150‑1 (MQ=60)
                                                                                                                                               ATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCCGGGTGATTAAGACGAGACGGCTCGGATATG        >  SRR19973066.711763/1‑150 (MQ=60)
                                                                                                                                               ATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGTTATG        <  SRR19973066.1034752/150‑1 (MQ=60)
                                                                                                                                               ATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATG        <  SRR19973066.1032311/150‑1 (MQ=60)
                                                                                                                                               ATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATG        <  SRR19973066.1032424/150‑1 (MQ=60)
                                                                                                                                               ATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATG        >  SRR19973066.711934/1‑150 (MQ=60)
                                                                                                                                               ATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATG        >  SRR19973066.1743220/1‑150 (MQ=60)
                                                                                                                                               ATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATG        <  SRR19973066.124639/150‑1 (MQ=60)
                                                                                                                                               ATTGAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATG        >  SRR19973066.3517571/1‑150 (MQ=60)
                                                                                                                                                  GAGAAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCCGGGTGATTAAGACGAGACGGCTCGGATATGTAG     >  SRR19973066.3495977/1‑150 (MQ=60)
                                                                                                                                                     AAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGT  <  SRR19973066.348029/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGT  >  CP009273/509588‑509886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑