Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 627,428 C→A 93.0% I693I (ATC→ATA)  cstA → carbon starvation protein involved in peptide utilization; APC peptide transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273627,4280CA93.0% 595.3 / 35.9 214I693I (ATC→ATA) cstAcarbon starvation protein involved in peptide utilization; APC peptide transporter family protein
Reads supporting (aligned to +/- strand):  ref base C (7/8);  new base A (109/90);  total (116/98)
Fisher's exact test for biased strand distribution p-value = 5.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

Only 100 of 215 total aligned reads displayed.

GGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGT  >  CP009273/627281‑627572
                                                                                                                                                   |                                                                                                                                                
ggATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGAGTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATc                                                                                                                                                  >  2:2105605/1‑148 (MQ=255)
    gCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTgg                                                                                                                                              >  1:1030685/1‑148 (MQ=255)
    gCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTgg                                                                                                                                              >  2:619463/1‑148 (MQ=255)
    gCCGGGTTAACCATCTTCGTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTgg                                                                                                                                              >  2:619415/1‑148 (MQ=255)
       gggTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGgcgc                                                                                                                                           <  1:1108605/148‑1 (MQ=255)
           tAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGc                                                                                                                                       >  1:370246/1‑148 (MQ=255)
           tAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGc                                                                                                                                       >  1:434431/1‑148 (MQ=255)
              ccATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCaaa                                                                                                                                    >  1:676784/1‑148 (MQ=255)
              ccATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGACACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCaaa                                                                                                                                    >  2:1790720/1‑148 (MQ=255)
              ccATCTTCTTTAGGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCaaa                                                                                                                                    <  1:1579205/148‑1 (MQ=255)
               cATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCaaaa                                                                                                                                   >  1:3365799/1‑148 (MQ=255)
                 tcttctTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAgg                                                                                                                                 >  1:3060566/1‑148 (MQ=255)
                   ttcttTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGgcg                                                                                                                               >  2:1189420/1‑148 (MQ=255)
                     cttTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGca                                                                                                                             >  1:2216487/1‑148 (MQ=255)
                     cttTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGca                                                                                                                             <  2:2703034/148‑1 (MQ=255)
                       ttATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGcaca                                                                                                                           <  2:1872722/148‑1 (MQ=255)
                          gggtggtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCTAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTa                                                                                                                        <  1:17246/147‑1 (MQ=255)
                           ggtggtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTaa                                                                                                                       >  2:2364586/1‑148 (MQ=255)
                              ggtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGt                                                                                                                    >  1:3432960/1‑148 (MQ=255)
                                tCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCa                                                                                                                  <  2:142445/148‑1 (MQ=255)
                                tCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCa                                                                                                                  <  2:2233320/148‑1 (MQ=255)
                                  gtggtgGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCaga                                                                                                                <  1:1967604/148‑1 (MQ=255)
                                      tggtTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAATGGCGCACACTAAAGTCAGAGTGa                                                                                                            >  1:55698/1‑148 (MQ=255)
                                           cTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAggggg                                                                                                       <  1:2166592/148‑1 (MQ=255)
                                                 cTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGTCGAAAGAAACGCCGTATGAACCAATGCCGTAAAATGTCGAGTAGATAGTGGCGCAGGCAAATGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGt                                                                                                 <  2:3059195/148‑1 (MQ=255)
                                                 cTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTAGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGt                                                                                                 >  2:295940/1‑148 (MQ=255)
                                                   gTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTg                                                                                               >  1:2198968/1‑148 (MQ=255)
                                                     tctcGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGc                                                                                             >  2:1646520/1‑148 (MQ=255)
                                                          aTTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATg                                                                                        >  1:644480/1‑148 (MQ=255)
                                                          aTTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATg                                                                                        <  2:3140065/148‑1 (MQ=255)
                                                           ttAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGt                                                                                       <  1:1446282/148‑1 (MQ=255)
                                                            tAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGACTGtt                                                                                      >  1:25521/1‑148 (MQ=255)
                                                            tAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGtt                                                                                      >  1:964571/1‑148 (MQ=255)
                                                            tAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGtt                                                                                      >  1:3279031/1‑148 (MQ=255)
                                                            tAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGtt                                                                                      >  1:3200492/1‑148 (MQ=255)
                                                              aGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTgg                                                                                    >  1:790262/1‑148 (MQ=255)
                                                              aGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTgg                                                                                    <  1:1079815/148‑1 (MQ=255)
                                                              aGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTgg                                                                                    <  1:1079852/148‑1 (MQ=255)
                                                               gACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGc                                                                                   >  2:463897/1‑148 (MQ=255)
                                                                aCGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCt                                                                                  >  1:1292488/1‑148 (MQ=255)
                                                                   gCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAg                                                                               <  1:2484766/148‑1 (MQ=255)
                                                                   gCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAg                                                                               >  2:1213704/1‑148 (MQ=255)
                                                                   gCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAg                                                                               >  1:2043752/1‑148 (MQ=255)
                                                                     aCTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGCTGTTGGCGAATGTTGGCTTAGTg                                                                             >  2:1911572/1‑148 (MQ=255)
                                                                      cTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGc                                                                            >  2:2343647/1‑148 (MQ=255)
                                                                         gCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCa                                                                         <  1:176838/148‑1 (MQ=255)
                                                                         gCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCa                                                                         <  1:149080/148‑1 (MQ=255)
                                                                           ggCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAgg                                                                       >  2:3351449/1‑148 (MQ=255)
                                                                            gCATTGAAAGAACCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAggg                                                                      <  1:1824403/148‑1 (MQ=255)
                                                                             cATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGt                                                                     >  1:1391805/1‑148 (MQ=255)
                                                                                tGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTcc                                                                  >  1:3223514/1‑148 (MQ=255)
                                                                                  aaaGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCct                                                                >  1:2053724/1‑148 (MQ=255)
                                                                                  aaaGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCct                                                                <  1:2364344/148‑1 (MQ=255)
                                                                                    aGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCctct                                                              >  2:3194683/1‑148 (MQ=255)
                                                                                       tCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAc                                                           >  1:1219646/1‑148 (MQ=255)
                                                                                       tCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAc                                                           >  1:2992756/1‑148 (MQ=255)
                                                                                          gAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCt                                                        >  2:235462/1‑148 (MQ=255)
                                                                                            aGCCAACGGCTAAATACACGCCGTATGAACCAATGCCGAAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTaa                                                      <  1:1594467/148‑1 (MQ=255)
                                                                                              ccAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAAcc                                                    >  2:777053/1‑148 (MQ=255)
                                                                                                aaCGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCct                                                  >  2:2575701/1‑148 (MQ=255)
                                                                                                  cGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCctct                                                >  2:3460702/1‑148 (MQ=255)
                                                                                                       aaagaaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCg                                           >  1:246333/1‑148 (MQ=255)
                                                                                                          gaaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTg                                        >  1:604871/1‑148 (MQ=255)
                                                                                                          gaaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTACCCGGTg                                        >  2:2853631/1‑148 (MQ=255)
                                                                                                           aaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTgg                                       >  2:3197225/1‑148 (MQ=255)
                                                                                                           aaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTgg                                       >  1:49507/1‑148 (MQ=255)
                                                                                                            aaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTggg                                      >  2:2289252/1‑148 (MQ=255)
                                                                                                            aaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTggg                                      >  2:1570357/1‑148 (MQ=255)
                                                                                                              cGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGc                                    >  2:3490652/1‑148 (MQ=255)
                                                                                                                   tATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAggg                               >  1:302540/1‑148 (MQ=255)
                                                                                                                   tATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGAGGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAggg                               <  1:2911039/148‑1 (MQ=255)
                                                                                                                     tGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGa                             >  1:1159403/1‑148 (MQ=255)
                                                                                                                     tGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGa                             <  1:2796054/148‑1 (MQ=255)
                                                                                                                       aaCCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACt                           <  2:932282/148‑1 (MQ=255)
                                                                                                                       aaCCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACt                           >  2:583300/1‑148 (MQ=255)
                                                                                                                         ccAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGa                         <  1:1363186/148‑1 (MQ=255)
                                                                                                                         ccAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGa                         >  1:189242/1‑148 (MQ=255)
                                                                                                                           aaTGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGAcc                       <  2:215689/148‑1 (MQ=255)
                                                                                                                             tGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGa                     >  2:1983455/1‑148 (MQ=255)
                                                                                                                              gCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAg                    >  2:1483767/1‑148 (MQ=255)
                                                                                                                                  gAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGcgcg                >  2:2662779/1‑148 (MQ=255)
                                                                                                                                  gAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGcgcg                <  2:3239700/148‑1 (MQ=255)
                                                                                                                                  gAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGcgcg                <  2:1649148/148‑1 (MQ=255)
                                                                                                                                   aaaaTGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGt               >  1:2078534/1‑148 (MQ=255)
                                                                                                                                   aaaaTGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGt               >  2:2107105/1‑148 (MQ=255)
                                                                                                                                    aaaTGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGtt              >  1:1844006/1‑148 (MQ=255)
                                                                                                                                     aaTGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTg             <  1:177746/148‑1 (MQ=255)
                                                                                                                                     aaTGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTg             <  1:2430922/148‑1 (MQ=255)
                                                                                                                                      aTGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGa            <  2:2636104/148‑1 (MQ=255)
                                                                                                                                       tGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGAt           <  2:2301425/148‑1 (MQ=255)
                                                                                                                                       tGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGAt           <  1:871914/148‑1 (MQ=255)
                                                                                                                                         tCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAg         <  2:3279031/148‑1 (MQ=255)
                                                                                                                                         tCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAg         >  2:233014/1‑148 (MQ=255)
                                                                                                                                            aggagATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCAt      >  2:429427/1‑148 (MQ=255)
                                                                                                                                            aggagATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCAt      >  2:429424/1‑148 (MQ=255)
                                                                                                                                              gagaTAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCAttt    >  1:650447/1‑148 (MQ=255)
                                                                                                                                               agaTAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTg   <  2:434431/148‑1 (MQ=255)
                                                                                                                                               agaTAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTg   >  2:61005/1‑148 (MQ=255)
                                                                                                                                               agaTAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTg   <  2:370246/148‑1 (MQ=255)
                                                                                                                                                gaTAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGt  <  1:2073054/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGT  >  CP009273/627281‑627572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCC  >  CP009273/627280‑627577
                                                                                                                                                    |                                                                                                                                                     
TGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAG                                                                                                                                                      >  SRR19973067.1600322/1‑150 (MQ=60)
TGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAG                                                                                                                                                      >  SRR19973067.2152584/1‑150 (MQ=60)
TGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAG                                                                                                                                                      >  SRR19973067.2325013/1‑150 (MQ=60)
  GATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTG                                                                                                                                                    <  SRR19973067.1422016/150‑1 (MQ=60)
   ATGCGGGGTTAACCATCTTCTTTATGGTGGTGGTGGTGGTTCTGGCAGTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGG                                                                                                                                                   <  SRR19973067.2916626/150‑1 (MQ=60)
   ATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGG                                                                                                                                                   >  SRR19973067.1037778/1‑150 (MQ=60)
        GGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAG                                                                                                                                              <  SRR19973067.1116131/150‑1 (MQ=60)
          GTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGC                                                                                                                                            >  SRR19973067.372823/1‑150 (MQ=60)
          GTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGC                                                                                                                                            >  SRR19973067.437449/1‑150 (MQ=60)
          GTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGC                                                                                                                                            >  SRR19973067.175215/1‑150 (MQ=60)
             AACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAA                                                                                                                                         >  SRR19973067.681429/1‑150 (MQ=60)
              ACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAA                                                                                                                                        >  SRR19973067.3387164/1‑150 (MQ=60)
               CCATCTTCTTTAGGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAG                                                                                                                                       <  SRR19973067.1590015/150‑1 (MQ=60)
                CATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGG                                                                                                                                      >  SRR19973067.3080051/1‑150 (MQ=60)
                  TCTTCTTTATGGTGGTCGTGGGGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAACGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCG                                                                                                                                    <  SRR19973067.1370093/150‑1 (MQ=60)
                   CTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGC                                                                                                                                   >  SRR19973067.1216028/1‑150 (MQ=60)
                   CTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGC                                                                                                                                   >  SRR19973067.157749/1‑150 (MQ=60)
                    TTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCA                                                                                                                                  >  SRR19973067.2231249/1‑150 (MQ=60)
                     TCTTTATGGTGGTCGTGGTGGTTCTGGCAGTGTTATCTATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCACCGGCTAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCAC                                                                                                                                 <  SRR19973067.24943/150‑1 (MQ=60)
                       TTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACAC                                                                                                                               >  SRR19973067.872046/1‑150 (MQ=60)
                       TTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACAC                                                                                                                               >  SRR19973067.412924/1‑150 (MQ=60)
                        TTATGGTGGTGGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACT                                                                                                                              <  SRR19973067.3077694/150‑1 (MQ=60)
                           GGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCTAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAA                                                                                                                           <  SRR19973067.17508/150‑1 (MQ=60)
                            GGGGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCACCGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAG                                                                                                                          <  SRR19973067.1121860/150‑1 (MQ=60)
                             GTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGT                                                                                                                         >  SRR19973067.3454813/1‑150 (MQ=60)
                                   GTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGT                                                                                                                   <  SRR19973067.1980959/150‑1 (MQ=60)
                                     GGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAATGGCGCACACTAAAGTCAGAGTGA                                                                                                                 >  SRR19973067.56404/1‑150 (MQ=60)
                                        GGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGG                                                                                                              <  SRR19973067.1104197/150‑1 (MQ=60)
                                           TCTGGCACTGTTCTCTATTAAGACGGCACTTGCGGCATTTAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGG                                                                                                           <  SRR19973067.719824/150‑1 (MQ=60)
                                            CTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCTAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGG                                                                                                          <  SRR19973067.2181725/150‑1 (MQ=60)
                                            CTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGG                                                                                                          <  SRR19973067.2181081/150‑1 (MQ=60)
                                             TGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGA                                                                                                         <  SRR19973067.1900036/150‑1 (MQ=60)
                                             TGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGA                                                                                                         >  SRR19973067.2942720/1‑150 (MQ=60)
                                             TGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGA                                                                                                         <  SRR19973067.3184753/150‑1 (MQ=60)
                                              GGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGAT                                                                                                        <  SRR19973067.1001302/150‑1 (MQ=60)
                                                CACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGT                                                                                                      <  SRR19973067.3222464/150‑1 (MQ=60)
                                                  CTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTG                                                                                                    >  SRR19973067.2213638/1‑150 (MQ=60)
                                                         CGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATG                                                                                             >  SRR19973067.648833/1‑150 (MQ=60)
                                                          GATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGT                                                                                            >  SRR19973067.537325/1‑150 (MQ=60)
                                                           ATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGACTGTT                                                                                           >  SRR19973067.25913/1‑150 (MQ=60)
                                                           ATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTT                                                                                           >  SRR19973067.3220680/1‑150 (MQ=60)
                                                           ATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTT                                                                                           >  SRR19973067.3299542/1‑150 (MQ=60)
                                                           ATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTT                                                                                           >  SRR19973067.971182/1‑150 (MQ=60)
                                                           ATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTT                                                                                           >  SRR19973067.3299576/1‑150 (MQ=60)
                                                            TTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTG                                                                                          <  SRR19973067.1455988/150‑1 (MQ=60)
                                                             TAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGG                                                                                         >  SRR19973067.795726/1‑150 (MQ=60)
                                                               AGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCT                                                                                       <  SRR19973067.1087246/150‑1 (MQ=60)
                                                               AGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCT                                                                                       <  SRR19973067.1087209/150‑1 (MQ=60)
                                                               AGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCT                                                                                       >  SRR19973067.1301335/1‑150 (MQ=60)
                                                               AGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCT                                                                                       >  SRR19973067.1301312/1‑150 (MQ=60)
                                                               AGACGGCACTTGCGGAATTGAAAGATCCGAAGCCAACGGCTAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCT                                                                                       <  SRR19973067.1087221/150‑1 (MQ=60)
                                                                GACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTT                                                                                      >  SRR19973067.1845821/1‑150 (MQ=60)
                                                                 ACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTA                                                                                     <  SRR19973067.2360326/150‑1 (MQ=60)
                                                                  CGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAG                                                                                    >  SRR19973067.2057423/1‑150 (MQ=60)
                                                                  CGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAG                                                                                    >  SRR19973067.2057417/1‑150 (MQ=60)
                                                                   GGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGT                                                                                   <  SRR19973067.2764307/150‑1 (MQ=60)
                                                                   GGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGT                                                                                   <  SRR19973067.648887/150‑1 (MQ=60)
                                                                   GGCACTGGCGGCATTTACAGATCCGAAGCCAACGGCGACAGACACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGT                                                                                   <  SRR19973067.648490/150‑1 (MQ=60)
                                                                    GCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTG                                                                                  <  SRR19973067.2501098/150‑1 (MQ=60)
                                                                    GCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTG                                                                                  <  SRR19973067.2500389/150‑1 (MQ=60)
                                                                    GCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTG                                                                                  >  SRR19973067.1272420/1‑150 (MQ=60)
                                                                         TGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAG                                                                             <  SRR19973067.3461828/150‑1 (MQ=60)
                                                                          GCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGG                                                                            <  SRR19973067.1987998/150‑1 (MQ=60)
                                                                          GCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGG                                                                            <  SRR19973067.178252/150‑1 (MQ=60)
                                                                          GCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGG                                                                            <  SRR19973067.150350/150‑1 (MQ=60)
                                                                          GCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGG                                                                            <  SRR19973067.150362/150‑1 (MQ=60)
                                                                           CGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTAGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGG                                                                           <  SRR19973067.1957885/150‑1 (MQ=60)
                                                                            GGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGT                                                                          >  SRR19973067.1401265/1‑150 (MQ=60)
                                                                             GCATTGAAAGAGCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTT                                                                         <  SRR19973067.2530035/150‑1 (MQ=60)
                                                                             GCATTGAAAGAACCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTT                                                                         <  SRR19973067.1836786/150‑1 (MQ=60)
                                                                               ATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCC                                                                       >  SRR19973067.3243770/1‑150 (MQ=60)
                                                                                 TGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCT                                                                     <  SRR19973067.3322305/150‑1 (MQ=60)
                                                                                 TGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCT                                                                     >  SRR19973067.2067427/1‑150 (MQ=60)
                                                                                   AAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCT                                                                   <  SRR19973067.2379989/150‑1 (MQ=60)
                                                                                   AAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCT                                                                   <  SRR19973067.2379745/150‑1 (MQ=60)
                                                                                    AAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTC                                                                  >  SRR19973067.143337/1‑150 (MQ=60)
                                                                                    AAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTC                                                                  >  SRR19973067.1753605/1‑150 (MQ=60)
                                                                                     AGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCA                                                                 >  SRR19973067.3451181/1‑150 (MQ=60)
                                                                                     AGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCA                                                                 >  SRR19973067.3310566/1‑150 (MQ=60)
                                                                                      GATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAC                                                                >  SRR19973067.3011721/1‑150 (MQ=60)
                                                                                      GATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAC                                                                >  SRR19973067.1227756/1‑150 (MQ=60)
                                                                                             AGCCAACGGCTAAATACACGCCGTATGAACCAATGCCGAAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACC                                                         <  SRR19973067.1605396/150‑1 (MQ=60)
                                                                                             AGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACC                                                         <  SRR19973067.1802786/150‑1 (MQ=60)
                                                                                               CCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCT                                                       <  SRR19973067.2360101/150‑1 (MQ=60)
                                                                                                 AACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTGGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCT                                                     <  SRR19973067.3009943/150‑1 (MQ=60)
                                                                                                      CGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCG                                                >  SRR19973067.1789025/1‑150 (MQ=60)
                                                                                                      CGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCG                                                <  SRR19973067.1657859/150‑1 (MQ=60)
                                                                                                      CGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCG                                                >  SRR19973067.248043/1‑150 (MQ=60)
                                                                                                        AAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGT                                              >  SRR19973067.1984323/1‑150 (MQ=60)
                                                                                                         AAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTG                                             >  SRR19973067.608727/1‑150 (MQ=60)
                                                                                                         AAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTG                                             >  SRR19973067.608998/1‑150 (MQ=60)
                                                                                                          AGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGG                                            >  SRR19973067.50145/1‑150 (MQ=60)
                                                                                                          AGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGG                                            >  SRR19973067.50160/1‑150 (MQ=60)
                                                                                                          AGAAACGCAGTATGAACCAATGCCGGAAAATGTAGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGTGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGG                                            <  SRR19973067.2222358/150‑1 (MQ=60)
                                                                                                                 CCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGG                                     <  SRR19973067.663017/150‑1 (MQ=60)
                                                                                                                  CGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGG                                    >  SRR19973067.304484/1‑150 (MQ=60)
                                                                                                                    TATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGA                                  >  SRR19973067.1167126/1‑150 (MQ=60)
                                                                                                                    TATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGAGGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGA                                  <  SRR19973067.2929585/150‑1 (MQ=60)
                                                                                                                      TGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACT                                <  SRR19973067.2814087/150‑1 (MQ=60)
                                                                                                                       GAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTG                               >  SRR19973067.1044933/1‑150 (MQ=60)
                                                                                                                        AACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGA                              >  SRR19973067.190714/1‑150 (MQ=60)
                                                                                                                          CCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACC                            <  SRR19973067.1373043/150‑1 (MQ=60)
                                                                                                                          CCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACC                            <  SRR19973067.1372495/150‑1 (MQ=60)
                                                                                                                            AATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGACTGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGA                          >  SRR19973067.909872/1‑150 (MQ=60)
                                                                                                                                 CGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCG                     <  SRR19973067.3025929/150‑1 (MQ=60)
                                                                                                                                  GGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGT                    >  SRR19973067.2092317/1‑150 (MQ=60)
                                                                                                                                   GAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTT                   >  SRR19973067.1856580/1‑150 (MQ=60)
                                                                                                                                      AATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGAT                <  SRR19973067.179167/150‑1 (MQ=60)
                                                                                                                                      AATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGAT                <  SRR19973067.2446880/150‑1 (MQ=60)
                                                                                                                                        TGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAG              <  SRR19973067.2119582/150‑1 (MQ=60)
                                                                                                                                        TGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAG              <  SRR19973067.1601474/150‑1 (MQ=60)
                                                                                                                                        TGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAG              <  SRR19973067.877734/150‑1 (MQ=60)
                                                                                                                                             AGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTT         >  SRR19973067.654860/1‑150 (MQ=60)
                                                                                                                                             AGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTT         >  SRR19973067.577086/1‑150 (MQ=60)
                                                                                                                                                 GATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAG     <  SRR19973067.2086847/150‑1 (MQ=60)
                                                                                                                                                 GATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAG     <  SRR19973067.2086820/150‑1 (MQ=60)
                                                                                                                                                 GATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAG     <  SRR19973067.2086817/150‑1 (MQ=60)
                                                                                                                                                  ATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGG    >  SRR19973067.3528131/1‑150 (MQ=60)
                                                                                                                                                    AGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCC  >  SRR19973067.77454/1‑150 (MQ=60)
                                                                                                                                                    AGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCC  >  SRR19973067.1888272/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCC  >  CP009273/627280‑627577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑