Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 627,428 C→A 100% I693I (ATC→ATA)  cstA → carbon starvation protein involved in peptide utilization; APC peptide transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273627,4280CA98.2% 709.1 / 3.1 227I693I (ATC→ATA) cstAcarbon starvation protein involved in peptide utilization; APC peptide transporter family protein
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (115/108);  total (117/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 227 total aligned reads displayed.

ATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGT  >  CP009273/627283‑627572
                                                                                                                                                 |                                                                                                                                                
   ccGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGgc                                                                                                                                             >  1:606086/1‑148 (MQ=255)
       gTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAg                                                                                                                                         >  2:170957/1‑148 (MQ=255)
        ttAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAgg                                                                                                                                        >  2:2957914/1‑148 (MQ=255)
        ttAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAgg                                                                                                                                        >  2:3106872/1‑148 (MQ=255)
           aCCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAATACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAAAGCCGTATGAACCAATGCCCGAAAATGTCGAGGAGATAGTGGCGCAGGCaa                                                                                                                                     >  1:2205394/1‑148 (MQ=255)
            ccATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCaaa                                                                                                                                    >  1:884573/1‑148 (MQ=255)
             cATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCaaaa                                                                                                                                   >  2:685920/1‑148 (MQ=255)
             cATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCaaaa                                                                                                                                   >  1:294336/1‑148 (MQ=255)
             cATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCaaaa                                                                                                                                   <  2:2004937/148‑1 (MQ=255)
             cATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCaaaa                                                                                                                                   >  1:879250/1‑148 (MQ=255)
                cttcttTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGgc                                                                                                                                >  1:2731461/1‑148 (MQ=255)
                 ttcttTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGgcg                                                                                                                               >  1:456976/1‑148 (MQ=255)
                     ttctggtggTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATGGAAAGATCCGAAGCCAACGGCGACAGAACCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGcaca                                                                                                                           <  2:3358945/145‑1 (MQ=255)
                       aTGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACt                                                                                                                         >  2:945456/1‑148 (MQ=255)
                        tggtggTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTa                                                                                                                        <  2:743705/148‑1 (MQ=255)
                            ggtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGt                                                                                                                    <  2:762453/148‑1 (MQ=255)
                             gtCGTGGTGGTTCTGGCACTGTTCTCTATTAAGACGGCACTTGCGGCTTTTAAAGTTCCGATGCCAACGGCGAAAGAAACTCCGTATGAACCAATGACGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTc                                                                                                                   <  2:3010674/148‑1 (MQ=255)
                              tggtggtgGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCa                                                                                                                  <  2:2408934/146‑1 (MQ=255)
                              tCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCa                                                                                                                  >  2:3556580/1‑148 (MQ=255)
                               cGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCTAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCag                                                                                                                 <  1:1539800/148‑1 (MQ=255)
                               cGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCag                                                                                                                 <  1:1993277/148‑1 (MQ=255)
                                 tggtggTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCagag                                                                                                               >  2:3018888/1‑148 (MQ=255)
                                 tggtggTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCagag                                                                                                               >  2:1243482/1‑148 (MQ=255)
                                   gtggtTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTg                                                                                                             <  2:1903472/148‑1 (MQ=255)
                                   gtggtTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTg                                                                                                             <  2:3262016/148‑1 (MQ=255)
                                    tggtTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGa                                                                                                            <  1:2789899/148‑1 (MQ=255)
                                      gtTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGAATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAgg                                                                                                          <  1:662035/148‑1 (MQ=255)
                                       ttCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAggg                                                                                                         >  1:2354786/1‑148 (MQ=255)
                                       ttCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAggg                                                                                                         <  1:3169672/148‑1 (MQ=255)
                                       ttCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAggg                                                                                                         <  1:1399898/148‑1 (MQ=255)
                                           ggCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAACATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAggggggg                                                                                                     >  2:2273780/1‑148 (MQ=255)
                                              aCTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGATGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATg                                                                                                  <  2:3169008/148‑1 (MQ=255)
                                              aCTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATg                                                                                                  <  2:1391693/148‑1 (MQ=255)
                                                        aTTAAGACGGCACTTTCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATg                                                                                        <  2:2246361/148‑1 (MQ=255)
                                                        aTTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCGGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATg                                                                                        >  2:1728824/1‑148 (MQ=255)
                                                         ttAAGACGGCTCTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGt                                                                                       <  1:170957/148‑1 (MQ=255)
                                                         ttAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGt                                                                                       <  1:1319642/148‑1 (MQ=255)
                                                          tAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGtt                                                                                      <  1:2995664/148‑1 (MQ=255)
                                                           aaGACGGCACTTGCGGCTTTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTg                                                                                     <  1:1249386/148‑1 (MQ=255)
                                                           aaGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTg                                                                                     <  1:3025262/148‑1 (MQ=255)
                                                           aaGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTg                                                                                     <  2:454577/148‑1 (MQ=255)
                                                             gACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCACAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGc                                                                                   >  2:2363543/1‑148 (MQ=255)
                                                             gACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGc                                                                                   <  1:1072314/148‑1 (MQ=255)
                                                             gACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGc                                                                                   <  1:1114526/148‑1 (MQ=255)
                                                             gACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGc                                                                                   >  1:2257808/1‑148 (MQ=255)
                                                                 gCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAg                                                                               <  2:3043626/148‑1 (MQ=255)
                                                                 gCACGTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAg                                                                               <  2:3042283/148‑1 (MQ=255)
                                                                     ttGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGcc                                                                           <  1:1949127/148‑1 (MQ=255)
                                                                     ttGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGcc                                                                           <  2:2457240/148‑1 (MQ=255)
                                                                      tGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGCTGTTGGCGAATGTTGGCTTAGTGccc                                                                          >  1:1061643/1‑148 (MQ=255)
                                                                          gCATTGAAAGATCCGAAGCCAACGGCGAAAGACACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAggg                                                                      <  1:3327148/148‑1 (MQ=255)
                                                                              tGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTcc                                                                  <  2:3365048/148‑1 (MQ=255)
                                                                                aaaGATCCGAAGCCACCGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCct                                                                <  2:1102262/148‑1 (MQ=255)
                                                                                aaaGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCct                                                                >  1:2920709/1‑148 (MQ=255)
                                                                                 aaGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCctc                                                               >  1:510596/1‑148 (MQ=255)
                                                                                   gATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGATTAGTGCCCAGGGTTCCCtctc                                                             >  2:91205/1‑148 (MQ=255)
                                                                                     tCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAc                                                           <  1:3353563/148‑1 (MQ=255)
                                                                                     tCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAc                                                           >  2:2268119/1‑148 (MQ=255)
                                                                                      ccGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAcc                                                          <  2:479764/148‑1 (MQ=255)
                                                                                         aaGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTa                                                       >  1:3392775/1‑148 (MQ=255)
                                                                                            ccAACGGCGAAAGAACCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGGTCCCTCTCACCCTAAcc                                                    <  2:2205394/148‑1 (MQ=255)
                                                                                            ccAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAAcc                                                    >  2:1088793/1‑148 (MQ=255)
                                                                                            ccAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAAcc                                                    >  1:1426047/1‑148 (MQ=255)
                                                                                            ccAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAAcc                                                    <  2:1372248/148‑1 (MQ=255)
                                                                                            ccAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAAcc                                                    >  1:2564588/1‑148 (MQ=255)
                                                                                             cAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAAccc                                                   >  1:2977314/1‑148 (MQ=255)
                                                                                              aaCGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACACTAACCct                                                  <  1:2238919/148‑1 (MQ=255)
                                                                                                 ggCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCtctc                                               <  2:2825033/148‑1 (MQ=255)
                                                                                                   cGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTccc                                             >  1:1147531/1‑148 (MQ=255)
                                                                                                        gaaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTg                                        >  2:1546517/1‑148 (MQ=255)
                                                                                                        gaaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGAGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGAGCCCAGGGTTCCCTCTCACCCTAAACCTCTCCCCGGTg                                        >  2:259231/1‑148 (MQ=255)
                                                                                                          aaCGCCGTATGAACCAATGACGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTggg                                      >  1:2390985/1‑148 (MQ=255)
                                                                                                              ccGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGa                                  <  2:2553048/148‑1 (MQ=255)
                                                                                                              ccGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGa                                  <  2:2843133/148‑1 (MQ=255)
                                                                                                               cGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAg                                 >  1:3200622/1‑148 (MQ=255)
                                                                                                               cGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAg                                 >  1:2380349/1‑148 (MQ=255)
                                                                                                                gTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAgg                                >  1:3214475/1‑148 (MQ=255)
                                                                                                                 tATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAggg                               <  1:3106872/148‑1 (MQ=255)
                                                                                                                 tATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAggg                               <  1:1604352/148‑1 (MQ=255)
                                                                                                                  aTGAACCACTGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAgggg                              <  2:689706/148‑1 (MQ=255)
                                                                                                                   tGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGa                             <  2:606086/148‑1 (MQ=255)
                                                                                                                   tGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGa                             >  1:567342/1‑148 (MQ=255)
                                                                                                                     aaCCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACt                           <  2:2165006/148‑1 (MQ=255)
                                                                                                                      aCCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTg                          >  2:2563594/1‑148 (MQ=255)
                                                                                                                      aCCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTg                          <  2:3445035/148‑1 (MQ=255)
                                                                                                                      aCCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTg                          <  2:2074180/148‑1 (MQ=255)
                                                                                                                       ccAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGa                         >  1:2028603/1‑148 (MQ=255)
                                                                                                                        cAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGAc                        <  1:2089593/148‑1 (MQ=255)
                                                                                                                           tGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGa                     <  2:2257808/148‑1 (MQ=255)
                                                                                                                           tGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGa                     >  2:1680845/1‑148 (MQ=255)
                                                                                                                              cGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAgcg                  <  1:1835108/148‑1 (MQ=255)
                                                                                                                                gAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGcgcg                >  1:2560652/1‑148 (MQ=255)
                                                                                                                                 aaaaTGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGt               >  2:3299733/1‑148 (MQ=255)
                                                                                                                                   aaTGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCC‑AACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGa            >  1:474100/1‑148 (MQ=255)
                                                                                                                                     tGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGAt           >  2:1467663/1‑148 (MQ=255)
                                                                                                                                     tGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGAt           >  2:846845/1‑148 (MQ=255)
                                                                                                                                     tGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGAt           <  1:2677267/148‑1 (MQ=255)
                                                                                                                                          aggagATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCAt      >  1:3422259/1‑148 (MQ=255)
                                                                                                                                           ggagATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATCGCAtt     >  1:2421460/1‑148 (MQ=255)
                                                                                                                                              gaTAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGt  >  2:1050062/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGT  >  CP009273/627283‑627572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCC  >  CP009273/627280‑627577
                                                                                                                                                    |                                                                                                                                                     
TGGATGCGGGTTTAACCAGCTTCTTTAGGGTGGTGGTGGTGGTTGTGCCACTGTTCTCTATTAAGACGGCACTTGCGGCATTTAAAGATCCGAAGCAAACGGCGAAATAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAG                                                                                                                                                      <  SRR19973066.3337026/150‑1 (MQ=60)
TGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAG                                                                                                                                                      >  SRR19973066.891367/1‑150 (MQ=60)
 GGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGT                                                                                                                                                     >  SRR19973066.458071/1‑150 (MQ=60)
 GGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGT                                                                                                                                                     >  SRR19973066.2264818/1‑150 (MQ=60)
    TGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGC                                                                                                                                                  >  SRR19973066.610288/1‑150 (MQ=60)
    TGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGC                                                                                                                                                  >  SRR19973066.3156314/1‑150 (MQ=60)
        GGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAG                                                                                                                                              >  SRR19973066.3047963/1‑150 (MQ=60)
           TTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCA                                                                                                                                           <  SRR19973066.2176996/150‑1 (MQ=60)
            TAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAATACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAAAGCCGTATGAACCAATGCCCGAAAATGTCGAGGAGATAGTGGCGCAGGCAA                                                                                                                                          >  SRR19973066.2220677/1‑150 (MQ=60)
            TAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAA                                                                                                                                          >  SRR19973066.1382174/1‑150 (MQ=60)
             AACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAA                                                                                                                                         >  SRR19973066.890864/1‑150 (MQ=60)
             AACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAA                                                                                                                                         >  SRR19973066.2000490/1‑150 (MQ=60)
              ACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAATA                                                                                                                                        >  SRR19973066.885613/1‑150 (MQ=60)
              ACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAA                                                                                                                                        >  SRR19973066.885512/1‑150 (MQ=60)
              ACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAA                                                                                                                                        >  SRR19973066.296446/1‑150 (MQ=60)
              ACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAA                                                                                                                                        >  SRR19973066.3416131/1‑150 (MQ=60)
              ACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAA                                                                                                                                        >  SRR19973066.296433/1‑150 (MQ=60)
                 ATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGC                                                                                                                                     >  SRR19973066.2749836/1‑150 (MQ=60)
                  TCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCG                                                                                                                                    >  SRR19973066.460483/1‑150 (MQ=60)
                   CTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGC                                                                                                                                   >  SRR19973066.615711/1‑150 (MQ=60)
                        TTAGGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACT                                                                                                                              <  SRR19973066.2306424/150‑1 (MQ=60)
                             GTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGT                                                                                                                         <  SRR19973066.2191145/150‑1 (MQ=60)
                                  GGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCTAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAG                                                                                                                    <  SRR19973066.1550799/150‑1 (MQ=60)
                                  CGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAG                                                                                                                    <  SRR19973066.2007307/150‑1 (MQ=60)
                                  CGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAG                                                                                                                    <  SRR19973066.2007302/150‑1 (MQ=60)
                                      GTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAG                                                                                                                >  SRR19973066.274397/1‑150 (MQ=60)
                                       TTGTTCTGGCACTGTTCTCTATTAAGACGGCACTTGCGGCATTGAAATATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGG                                                                                                               <  SRR19973066.2808602/150‑1 (MQ=60)
                                       TGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGG                                                                                                               <  SRR19973066.2808675/150‑1 (MQ=60)
                                        GGTTCTGGCACTGTTCTCTATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGG                                                                                                              >  SRR19973066.3309487/1‑150 (MQ=60)
                                        GGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGG                                                                                                              >  SRR19973066.2370773/1‑150 (MQ=60)
                                         GTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG                                                                                                             >  SRR19973066.3387484/1‑150 (MQ=60)
                                         GTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGAATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG                                                                                                             <  SRR19973066.666869/150‑1 (MQ=60)
                                         GTTCTGGCACTGTTCTCGATTAAGACGGAACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGG                                                                                                             <  SRR19973066.2468159/150‑1 (MQ=60)
                                          TTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGACGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGG                                                                                                            <  SRR19973066.3041030/150‑1 (MQ=60)
                                          TTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGG                                                                                                            <  SRR19973066.1409984/150‑1 (MQ=60)
                                          TTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGG                                                                                                            <  SRR19973066.3190784/150‑1 (MQ=60)
                                           TCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGG                                                                                                           <  SRR19973066.928161/150‑1 (MQ=60)
                                              GGCACTGTTCTCTATTAAGACGGCACTTGCGGCATTGAAATATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGAT                                                                                                        <  SRR19973066.1297457/150‑1 (MQ=60)
                                                 AGTGTTCTCTATTAAGACGGCACTTGCGGAATTGAAATATCCGAAGCCACCGGCGAAACAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTT                                                                                                     <  SRR19973066.3013709/150‑1 (MQ=60)
                                                 ACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTT                                                                                                     <  SRR19973066.2052322/150‑1 (MQ=60)
                                                     TTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCG                                                                                                 <  SRR19973066.456811/150‑1 (MQ=60)
                                                          GATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGT                                                                                            >  SRR19973066.3357770/1‑150 (MQ=60)
                                                            TTAAGACGGCTCTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTG                                                                                          <  SRR19973066.172485/150‑1 (MQ=60)
                                                            TTAAGACGGCATTTGCGTCATTGAAAGATCCTCACCCAACGTCTAAACAACCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTG                                                                                          <  SRR19973066.162251/150‑1 (MQ=60)
                                                            TTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTG                                                                                          <  SRR19973066.1329205/150‑1 (MQ=60)
                                                             TAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGG                                                                                         <  SRR19973066.3015531/150‑1 (MQ=60)
                                                              AAGACGGCACTTGCGGCTTTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGC                                                                                        <  SRR19973066.1258356/150‑1 (MQ=60)
                                                              AAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGC                                                                                        <  SRR19973066.3045455/150‑1 (MQ=60)
                                                              AAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGCGGGATGTTGGCGAATGTTGGC                                                                                        >  SRR19973066.2273362/1‑150 (MQ=60)
                                                                GACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTT                                                                                      <  SRR19973066.1122572/150‑1 (MQ=60)
                                                                GACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTT                                                                                      <  SRR19973066.1122550/150‑1 (MQ=60)
                                                                GACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTT                                                                                      <  SRR19973066.1080172/150‑1 (MQ=60)
                                                                  CGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAG                                                                                    <  SRR19973066.1894673/150‑1 (MQ=60)
                                                                       CTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGCTGTTGGCGAATGTTGGCTTAGTGCCC                                                                               >  SRR19973066.1069430/1‑150 (MQ=60)
                                                                       CTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCC                                                                               >  SRR19973066.3163025/1‑150 (MQ=60)
                                                                       CTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCC                                                                               >  SRR19973066.1070327/1‑150 (MQ=60)
                                                                        TTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCA                                                                              <  SRR19973066.1962930/150‑1 (MQ=60)
                                                                           CGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGG                                                                           <  SRR19973066.601904/150‑1 (MQ=60)
                                                                             GCATTGAAAGATCCGAAGCCAACGGCGAAAGACACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTT                                                                         <  SRR19973066.3349251/150‑1 (MQ=60)
                                                                              CATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTC                                                                        >  SRR19973066.2833377/1‑150 (MQ=60)
                                                                                 TGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCT                                                                     >  SRR19973066.1150788/1‑150 (MQ=60)
                                                                                 TGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCT                                                                     >  SRR19973066.2940181/1‑150 (MQ=60)
                                                                                  GAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTC                                                                    >  SRR19973066.514375/1‑150 (MQ=60)
                                                                                  GAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTC                                                                    >  SRR19973066.514366/1‑150 (MQ=60)
                                                                                     AGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCA                                                                 >  SRR19973066.1709421/1‑150 (MQ=60)
                                                                                      GATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGTCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAC                                                                >  SRR19973066.1914386/1‑150 (MQ=60)
                                                                                        TCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCC                                                              <  SRR19973066.3375908/150‑1 (MQ=60)
                                                                                          CGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTA                                                            >  SRR19973066.3415414/1‑150 (MQ=60)
                                                                                             AGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACC                                                         >  SRR19973066.1436220/1‑150 (MQ=60)
                                                                                             AGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACC                                                         >  SRR19973066.2581996/1‑150 (MQ=60)
                                                                                             AGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACC                                                         >  SRR19973066.1436236/1‑150 (MQ=60)
                                                                                              GCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCC                                                        >  SRR19973066.2996990/1‑150 (MQ=60)
                                                                                                 AACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACACTAACCCTCT                                                     <  SRR19973066.2254360/150‑1 (MQ=60)
                                                                                                   CGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCC                                                   >  SRR19973066.1474947/1‑150 (MQ=60)
                                                                                                    GGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCC                                                  >  SRR19973066.1155718/1‑150 (MQ=60)
                                                                                                          AGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGG                                            >  SRR19973066.2233845/1‑150 (MQ=60)
                                                                                                           GAAACGCCGTATGAACCAATGACGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGG                                           >  SRR19973066.604801/1‑150 (MQ=60)
                                                                                                           GAAACGCCGTATGAACCAATGACGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGG                                           >  SRR19973066.2407057/1‑150 (MQ=60)
                                                                                                           GAAACGCCGTATGAACCAATGACGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGG                                           >  SRR19973066.2407075/1‑150 (MQ=60)
                                                                                                           GAAACGCCGTATGAACCAATGACGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGG                                           >  SRR19973066.2407077/1‑150 (MQ=60)
                                                                                                                GCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAG                                      >  SRR19973066.3221872/1‑150 (MQ=60)
                                                                                                                GCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAG                                      >  SRR19973066.3221876/1‑150 (MQ=60)
                                                                                                                GCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAG                                      >  SRR19973066.3221865/1‑150 (MQ=60)
                                                                                                                GCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAG                                      >  SRR19973066.2396405/1‑150 (MQ=60)
                                                                                                                GCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAG                                      >  SRR19973066.2396203/1‑150 (MQ=60)
                                                                                                                 CCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGG                                     >  SRR19973066.3235761/1‑150 (MQ=60)
                                                                                                                 CCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCACCGGTGGGGCGAGG                                     >  SRR19973066.1449615/1‑150 (MQ=60)
                                                                                                                   GTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGG                                   <  SRR19973066.2977513/150‑1 (MQ=60)
                                                                                                                   GTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGG                                   <  SRR19973066.2942150/150‑1 (MQ=60)
                                                                                                                    TATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGA                                  <  SRR19973066.1615997/150‑1 (MQ=60)
                                                                                                                    TATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGA                                  <  SRR19973066.3127619/150‑1 (MQ=60)
                                                                                                                    TATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGA                                  >  SRR19973066.571361/1‑150 (MQ=60)
                                                                                                                    TATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGA                                  >  SRR19973066.571282/1‑150 (MQ=60)
                                                                                                                     ATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGAC                                 <  SRR19973066.1254529/150‑1 (MQ=60)
                                                                                                                       GAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTG                               <  SRR19973066.3364707/150‑1 (MQ=60)
                                                                                                                        AACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGA                              >  SRR19973066.2042778/1‑150 (MQ=60)
                                                                                                                        AACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGA                              >  SRR19973066.1614936/1‑150 (MQ=60)
                                                                                                                         ACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGAC                             >  SRR19973066.1170878/1‑150 (MQ=60)
                                                                                                                           CAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCG                           <  SRR19973066.2103994/150‑1 (MQ=60)
                                                                                                                                 CGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCG                     >  SRR19973066.2578032/1‑150 (MQ=60)
                                                                                                                                 CGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCG                     >  SRR19973066.2627483/1‑150 (MQ=60)
                                                                                                                                 CGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCG                     <  SRR19973066.1848215/150‑1 (MQ=60)
                                                                                                                                 CGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCG                     >  SRR19973066.2578938/1‑150 (MQ=60)
                                                                                                                                 CGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCG                     >  SRR19973066.2297707/1‑150 (MQ=60)
                                                                                                                                    AAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCC‑AACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGA                 >  SRR19973066.477685/1‑150 (MQ=60)
                                                                                                                                    AAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGGGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCC‑AACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGA                 >  SRR19973066.477657/1‑150 (MQ=60)
                                                                                                                                    AAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGAGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGATG                  >  SRR19973066.3001343/1‑150 (MQ=60)
                                                                                                                                        TGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAG              <  SRR19973066.2695194/150‑1 (MQ=60)
                                                                                                                                        TGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAG              <  SRR19973066.898110/150‑1 (MQ=60)
                                                                                                                                        TGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAG              <  SRR19973066.2695143/150‑1 (MQ=60)
                                                                                                                                         GTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGC             <  SRR19973066.82313/150‑1 (MQ=60)
                                                                                                                                           CGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCAT           >  SRR19973066.3445147/1‑150 (MQ=60)
                                                                                                                                            GAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATCGCATT          >  SRR19973066.2437897/1‑150 (MQ=60)
                                                                                                                                            GAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATT          >  SRR19973066.2437904/1‑150 (MQ=60)
                                                                                                                                            GAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGAATT          >  SRR19973066.2097868/1‑150 (MQ=60)
                                                                                                                                             ATGAGATAGTGGCGCAGGCAAATGGCGCACACTAAAGTCAGTGTGAGGGGGGGATTTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTT         <  SRR19973066.1733711/150‑1 (MQ=60)
                                                                                                                                                   TAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGC   <  SRR19973066.2129066/150‑1 (MQ=60)
                                                                                                                                                   TAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGC   <  SRR19973066.2308256/150‑1 (MQ=60)
                                                                                                                                                    AGTGGCGCAGGCAACAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCC  <  SRR19973066.690960/150‑1 (MQ=60)
                                                                                                                                                    AGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCC  >  SRR19973066.1204360/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCC  >  CP009273/627280‑627577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑