Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,021,954 C→A 35.8% E298D (GAG→GAT)  yedI ← DUF808 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,021,9540CA35.8% 253.5 / 156.7 173E298D (GAG→GAT) yedIDUF808 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (51/59);  new base A (25/37);  total (76/97)
Fisher's exact test for biased strand distribution p-value = 5.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

Only 100 of 176 total aligned reads displayed.

GCCGCCCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGT  >  CP009273/2021807‑2022101
                                                                                                                                                    |                                                                                                                                                   
   gccCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTctct                                                                                                                                                  <  1:841780/148‑1 (MQ=255)
        ctTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGc                                                                                                                                             <  1:1254013/148‑1 (MQ=255)
          ttAACCGTAACGTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCtt                                                                                                                                           <  1:3313636/148‑1 (MQ=255)
            aaCCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTc                                                                                                                                         >  1:3248561/1‑148 (MQ=255)
             aCCGTAACTTCCTCACTAACCATACAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCa                                                                                                                                        <  1:2255062/148‑1 (MQ=255)
              ccGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCAc                                                                                                                                       >  1:346990/1‑148 (MQ=255)
                 tAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTcc                                                                                                                                    <  2:1501876/148‑1 (MQ=255)
                 tAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTcc                                                                                                                                    <  2:774739/148‑1 (MQ=255)
                 tAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTcc                                                                                                                                    <  1:3461443/148‑1 (MQ=255)
                  aaCTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTccc                                                                                                                                   <  1:389833/148‑1 (MQ=255)
                  aaCTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGGTCACCTTCGTTGAAGATGGCGAATGTTGCAGAAT‑AGCCAGTGCGCGTATTCCTGTGCCTGGCCGCGCCGTTGCTGTACGGCGTTCACTccc                                                                                                                                   >  2:2548374/1‑148 (MQ=255)
                   aCTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCa                                                                                                                                  >  2:1881692/1‑148 (MQ=255)
                    cTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAg                                                                                                                                 >  1:435687/1‑148 (MQ=255)
                      tCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGca                                                                                                                               >  2:3177416/1‑148 (MQ=255)
                      tCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGca                                                                                                                               >  1:65725/1‑148 (MQ=255)
                        cTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGcacc                                                                                                                             >  1:825301/1‑148 (MQ=255)
                        cTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGcacc                                                                                                                             >  2:2883706/1‑148 (MQ=255)
                              aaCCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATg                                                                                                                       <  2:1384713/148‑1 (MQ=255)
                               aCCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATgc                                                                                                                      >  2:2365675/1‑148 (MQ=255)
                               aCCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATgc                                                                                                                      >  1:619054/1‑148 (MQ=255)
                               aCCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATgc                                                                                                                      <  1:484217/148‑1 (MQ=255)
                                 cATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATgccg                                                                                                                    >  2:569002/1‑148 (MQ=255)
                                   tAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATgccgcc                                                                                                                  >  1:2533336/1‑148 (MQ=255)
                                    aaaGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGccgccg                                                                                                                 >  1:1138194/1‑148 (MQ=255)
                                     aaGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCga                                                                                                                <  2:733692/148‑1 (MQ=255)
                                      aGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCgat                                                                                                               >  2:2644153/1‑148 (MQ=255)
                                      aGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCgat                                                                                                               >  2:3546450/1‑148 (MQ=255)
                                      aGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCgat                                                                                                               >  2:2980532/1‑148 (MQ=255)
                                      aGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCgat                                                                                                               >  2:2980456/1‑148 (MQ=255)
                                       gCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCgatg                                                                                                              <  1:1445222/148‑1 (MQ=255)
                                           aTACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATaa                                                                                                          <  1:2242331/148‑1 (MQ=255)
                                           aTACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATaa                                                                                                          <  1:3080235/148‑1 (MQ=255)
                                            tACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATaaa                                                                                                         <  2:1168455/148‑1 (MQ=255)
                                            tACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATaaa                                                                                                         <  2:2519636/148‑1 (MQ=255)
                                             aCTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAAt                                                                                                        <  1:2294478/148‑1 (MQ=255)
                                              cTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGAAGGCGAATTTTGC‑TAATAAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATc                                                                                                       >  1:3490017/1‑148 (MQ=255)
                                                cggctcctTTTTTCGAGTGTAACTTGTTCACCTTAGTTGTAGATGGCGAATTTTGCATAAT‑AGCCAGTGCTAGTATTATTGTGCCTGACCGCGCATTTTGTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCa                                                                                                     <  2:505286/145‑1 (MQ=255)
                                                cGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCa                                                                                                     <  2:3033659/148‑1 (MQ=255)
                                                   ctcctTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGa                                                                                                  >  1:246373/1‑148 (MQ=255)
                                                   ctactTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGa                                                                                                  <  2:2134236/145‑1 (MQ=255)
                                                     cctTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAAt                                                                                                <  2:2414042/148‑1 (MQ=255)
                                                     cctTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAAt                                                                                                <  1:2372534/148‑1 (MQ=255)
                                                     cctTTTTTCGAGTGAAACTTGTTCAACTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAAt                                                                                                <  2:2414451/148‑1 (MQ=255)
                                                          tttCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGAt                                                                                           <  1:3403900/148‑1 (MQ=255)
                                                          tttCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGAt                                                                                           <  2:65725/148‑1 (MQ=255)
                                                          tttCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGAGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGAt                                                                                           <  2:1331485/148‑1 (MQ=255)
                                                              gAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTaa                                                                                       <  1:1067386/148‑1 (MQ=255)
                                                              gAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTaa                                                                                       <  1:2604317/148‑1 (MQ=255)
                                                                 tGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAAc                                                                                    <  1:60812/148‑1 (MQ=255)
                                                                 tGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACTATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAAc                                                                                    >  2:1739907/1‑148 (MQ=255)
                                                                 tGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAAc                                                                                    >  1:2116176/1‑148 (MQ=255)
                                                                  gAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTTACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACa                                                                                   >  2:3477996/1‑148 (MQ=255)
                                                                  gAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACa                                                                                   <  1:1871963/148‑1 (MQ=255)
                                                                     aCTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTc                                                                                <  1:3398611/148‑1 (MQ=255)
                                                                     aCTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTc                                                                                <  1:1174171/148‑1 (MQ=255)
                                                                     aCTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTc                                                                                <  1:985721/148‑1 (MQ=255)
                                                                      cTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCg                                                                               >  2:161492/1‑148 (MQ=255)
                                                                       ttGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCgg                                                                              >  1:2346235/1‑148 (MQ=255)
                                                                           tCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAat                                                                          <  1:809262/148‑1 (MQ=255)
                                                                           tCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAat                                                                          <  1:1888997/148‑1 (MQ=255)
                                                                                 tAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATatcatc                                                                    <  1:420097/148‑1 (MQ=255)
                                                                                     tGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCa                                                                >  1:1311355/1‑148 (MQ=255)
                                                                                       aaGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCact                                                              <  2:1665233/148‑1 (MQ=255)
                                                                                        aGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCacta                                                             <  2:1300709/148‑1 (MQ=255)
                                                                                         gATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCactac                                                            <  2:2127045/148‑1 (MQ=255)
                                                                                          aTGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCactact                                                           >  1:123571/1‑148 (MQ=255)
                                                                                           tGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCactacgg                                                          <  2:337856/148‑3 (MQ=255)
                                                                                           tGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTg                                                          <  2:337807/148‑1 (MQ=255)
                                                                                           tGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTg                                                          <  1:1037060/148‑1 (MQ=255)
                                                                                           tGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACAACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTg                                                          <  2:3259867/148‑1 (MQ=255)
                                                                                              cGAATTTTGCATAAG‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCAc                                                       <  2:2183746/148‑1 (MQ=255)
                                                                                               gAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACt                                                      <  2:3203595/148‑1 (MQ=255)
                                                                                                 aTTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACttt                                                    >  1:985443/1‑148 (MQ=255)
                                                                                                  ttttGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTtg                                                   <  1:151015/148‑1 (MQ=255)
                                                                                                  ttttGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTtg                                                   >  2:3147872/1‑148 (MQ=255)
                                                                                                  ttttGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTtg                                                   >  2:1631877/1‑148 (MQ=255)
                                                                                                  ttttGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTtg                                                   <  2:479252/148‑1 (MQ=255)
                                                                                                     tGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCCCTTtgctg                                                >  2:2431248/1‑148 (MQ=255)
                                                                                                      gCATAAT‑AGCCAGTGCGATTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGTTTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTtgctgc                                               <  1:1581447/148‑1 (MQ=255)
                                                                                                      gCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTtgctgc                                               <  1:156591/148‑1 (MQ=255)
                                                                                                       cATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGcc                                              >  1:3147298/1‑148 (MQ=255)
                                                                                                           at‑aGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGc                                          >  1:1998859/1‑148 (MQ=255)
                                                                                                              aGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGa                                        >  2:1893036/1‑148 (MQ=255)
                                                                                                                ccAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGaaa                                      >  1:1324449/1‑148 (MQ=255)
                                                                                                                  aGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATg                                    <  2:2761402/148‑1 (MQ=255)
                                                                                                                    tGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTATCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGtt                                  >  2:827201/1‑148 (MQ=255)
                                                                                                                     gCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTc                                 <  1:1589482/148‑1 (MQ=255)
                                                                                                                     gCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAAAAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGACATGTTc                                 >  2:1853309/1‑148 (MQ=255)
                                                                                                                          tATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATgg                            >  2:437629/1‑148 (MQ=255)
                                                                                                                             tAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGt                         >  1:1422986/1‑148 (MQ=255)
                                                                                                                                 gtgCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATg                     >  1:1735132/1‑148 (MQ=255)
                                                                                                                                   gCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCa                   >  1:2567440/1‑148 (MQ=255)
                                                                                                                                      tGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAgcg                <  2:1929636/148‑1 (MQ=255)
                                                                                                                                           gcgcATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCa           <  1:2850024/148‑1 (MQ=255)
                                                                                                                                               aTTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTc       >  2:1325176/1‑148 (MQ=255)
                                                                                                                                               aTTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTc       >  2:2620437/1‑148 (MQ=255)
                                                                                                                                                 tttCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATTATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCa     <  1:2980532/148‑1 (MQ=255)
                                                                                                                                                 tttCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCa     <  1:2980456/148‑1 (MQ=255)
                                                                                                                                                 tttATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCa     <  2:686213/148‑1 (MQ=255)
                                                                                                                                                    ctctACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAACTGGTCAATGGCGTGATGCAGCGGCGCACGTCCATGt  >  2:3244721/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCGCCCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAAT‑AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGT  >  CP009273/2021807‑2022101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A