Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,021,954 C→A 100% E298D (GAG→GAT)  yedI ← DUF808 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,021,9540CA98.3% 567.6 / 0.9 176E298D (GAG→GAT) yedIDUF808 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (90/83);  total (91/85)
Fisher's exact test for biased strand distribution p-value = 6.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 176 total aligned reads displayed.

CCCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCA  >  CP009273/2021811‑2022098
                                                                                                                                               |                                                                                                                                                
  ctctTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACg                                                                                                                                            >  1:2803519/1‑148 (MQ=255)
  ctctTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACg                                                                                                                                            >  1:1930399/1‑148 (MQ=255)
   tctTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACgg                                                                                                                                           >  1:550816/1‑148 (MQ=255)
   tctTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACgg                                                                                                                                           >  1:2984111/1‑148 (MQ=255)
    ctTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGc                                                                                                                                          <  1:775639/148‑1 (MQ=255)
    ctTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGc                                                                                                                                          <  1:928233/148‑1 (MQ=255)
       tAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCttt                                                                                                                                       >  1:2796501/1‑148 (MQ=255)
         aCCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCa                                                                                                                                     <  2:3076756/148‑1 (MQ=255)
           cGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACt                                                                                                                                   >  1:97498/1‑148 (MQ=255)
            gTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTc                                                                                                                                  >  1:776520/1‑148 (MQ=255)
              aaCTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTccc                                                                                                                                >  1:3496189/1‑148 (MQ=255)
                    cTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGcacc                                                                                                                          >  1:2640539/1‑148 (MQ=255)
                     tCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGcacca                                                                                                                         <  2:1305055/148‑1 (MQ=255)
                      cACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGcaccac                                                                                                                        >  1:1624177/1‑148 (MQ=255)
                       aCTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACg                                                                                                                       <  1:3209946/148‑1 (MQ=255)
                       aCTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATATTATCTACGGCTTTCACTCCCAGCACCACg                                                                                                                       >  2:2959096/1‑148 (MQ=255)
                           aacATACAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCGAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATgc                                                                                                                   <  2:2194270/146‑1 (MQ=255)
                           aCCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATgc                                                                                                                   <  2:591878/148‑1 (MQ=255)
                                aaaGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGccgccg                                                                                                              <  1:2151136/148‑1 (MQ=255)
                                aaaGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGccgccg                                                                                                              >  1:2403443/1‑148 (MQ=255)
                                aaaGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGccgccg                                                                                                              <  1:380250/148‑1 (MQ=255)
                                aaaGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGccgccg                                                                                                              >  1:1630571/1‑148 (MQ=255)
                                 aaGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCga                                                                                                             <  1:66009/148‑1 (MQ=255)
                                 aaGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCga                                                                                                             <  1:2800087/148‑1 (MQ=255)
                                    cTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCgatga                                                                                                          >  1:2279148/1‑148 (MQ=255)
                                     tCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCgatgat                                                                                                         >  1:1181881/1‑148 (MQ=255)
                                       aTACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATaa                                                                                                       >  1:1945100/1‑148 (MQ=255)
                                        tACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATaaa                                                                                                      <  1:831465/148‑1 (MQ=255)
                                        tACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATaaa                                                                                                      >  1:1480059/1‑148 (MQ=255)
                                         aCTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAAt                                                                                                     >  1:2100269/1‑148 (MQ=255)
                                         aCTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGAGGCCGCCGATGATAAAt                                                                                                     <  2:36007/148‑1 (MQ=255)
                                          cTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATc                                                                                                    >  1:277357/1‑148 (MQ=255)
                                          cTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATc                                                                                                    >  1:3095645/1‑148 (MQ=255)
                                              cctcctTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAg                                                                                                >  1:3182994/1‑148 (MQ=255)
                                               ctcctTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGa                                                                                               >  1:3007599/1‑148 (MQ=255)
                                               ctcctTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGa                                                                                               <  2:2885015/148‑1 (MQ=255)
                                                  ctTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATc                                                                                            <  2:3336879/148‑1 (MQ=255)
                                                  ctTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATc                                                                                            >  1:376880/1‑148 (MQ=255)
                                                  ctTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATc                                                                                            <  2:1809294/148‑1 (MQ=255)
                                                  ctTTTTTAGAGTGTAACTTTTTCAGCTTAGTTGAAGATGGCGAATTTTTCATAATAGCCAGTGCGATTATTAATGTGCCTTACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATc                                                                                            <  2:1809298/148‑1 (MQ=255)
                                                   ttttttCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCa                                                                                           >  1:3415016/1‑148 (MQ=255)
                                                    tttttCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAg                                                                                          <  2:674225/148‑1 (MQ=255)
                                                     ttttCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGa                                                                                         >  2:234803/1‑148 (MQ=255)
                                                      tttCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGAt                                                                                        <  2:2562173/148‑1 (MQ=255)
                                                       ttCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGAtt                                                                                       >  2:378518/1‑148 (MQ=255)
                                                         cGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTa                                                                                     <  1:3111781/148‑1 (MQ=255)
                                                            ttGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTaaaa                                                                                  <  2:120808/147‑1 (MQ=255)
                                                            gTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTaaaa                                                                                  <  2:120800/148‑1 (MQ=255)
                                                            gTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTaaaa                                                                                  <  2:2523114/148‑1 (MQ=255)
                                                             tGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAAc                                                                                 >  2:943562/1‑148 (MQ=255)
                                                              gAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACa                                                                                <  2:550816/148‑1 (MQ=255)
                                                                 aCTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTc                                                                             >  2:3309650/1‑148 (MQ=255)
                                                                  cTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCg                                                                            >  1:2558184/1‑148 (MQ=255)
                                                                  cTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCg                                                                            >  2:2575322/1‑148 (MQ=255)
                                                                    tGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGt                                                                          >  2:2624006/1‑148 (MQ=255)
                                                                    tGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGt                                                                          <  2:253785/148‑1 (MQ=255)
                                                                          ccTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATatc                                                                    >  1:269237/1‑148 (MQ=255)
                                                                          ccTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATatc                                                                    >  1:2519666/1‑148 (MQ=255)
                                                                          ccTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATatc                                                                    <  2:25758/148‑1 (MQ=255)
                                                                           cTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATatca                                                                   <  1:1876632/148‑1 (MQ=255)
                                                                             tAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATatcatc                                                                 >  2:351560/1‑148 (MQ=255)
                                                                             tAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATatcatc                                                                 >  2:351705/1‑148 (MQ=255)
                                                                             tAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATatcatc                                                                 >  2:481037/1‑148 (MQ=255)
                                                                              aGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCg                                                                <  1:1365574/148‑1 (MQ=255)
                                                                               gTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGc                                                               <  1:2243790/148‑1 (MQ=255)
                                                                                    aGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCacta                                                          <  1:3114731/148‑1 (MQ=255)
                                                                                     gATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCactac                                                         >  2:1427676/1‑148 (MQ=255)
                                                                                       tGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTg                                                       <  2:2035530/148‑1 (MQ=255)
                                                                                        ggCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGc                                                      >  1:819829/1‑148 (MQ=255)
                                                                                         gCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCa                                                     <  2:1398589/148‑1 (MQ=255)
                                                                                              ttttGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTtg                                                <  1:3245569/148‑1 (MQ=255)
                                                                                              ttttGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTtg                                                <  2:2729121/148‑1 (MQ=255)
                                                                                                   cATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGcc                                           <  2:3095645/148‑1 (MQ=255)
                                                                                                    ataataGCCAGTGCGAGTATTAATGTGCCTGACCGAGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGccc                                          <  2:1997155/148‑1 (MQ=255)
                                                                                                           ccAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGaaa                                   >  1:1009466/1‑148 (MQ=255)
                                                                                                            cAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAAt                                  <  2:1706463/148‑1 (MQ=255)
                                                                                                            cAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAAt                                  >  2:1505890/1‑148 (MQ=255)
                                                                                                               tGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGtt                               >  1:1016990/1‑148 (MQ=255)
                                                                                                               tGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGtt                               >  1:184112/1‑148 (MQ=255)
                                                                                                               tGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGtt                               >  2:3137456/1‑148 (MQ=255)
                                                                                                                 cGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCa                             <  1:250815/148‑1 (MQ=255)
                                                                                                                    gTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATg                          <  1:677361/148‑1 (MQ=255)
                                                                                                                     tATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATgg                         <  1:1530332/148‑1 (MQ=255)
                                                                                                                     tATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATgg                         <  1:2353957/148‑1 (MQ=255)
                                                                                                                       ttACTGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCg                       <  2:1811171/148‑1 (MQ=255)
                                                                                                                       ttAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCg                       <  2:1811323/148‑1 (MQ=255)
                                                                                                                       ttAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCg                       >  1:418757/1‑148 (MQ=255)
                                                                                                                        tAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGt                      >  2:1894707/1‑148 (MQ=255)
                                                                                                                         aaTGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTg                     <  2:1930399/148‑1 (MQ=255)
                                                                                                                          aTGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGa                    >  1:291307/1‑148 (MQ=255)
                                                                                                                          aTGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGa                    >  2:2433894/1‑148 (MQ=255)
                                                                                                                          aTGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGa                    >  1:109418/1‑148 (MQ=255)
                                                                                                                            gtgCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATg                  <  1:2511466/148‑1 (MQ=255)
                                                                                                                            gtgCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATg                  <  2:2838410/148‑1 (MQ=255)
                                                                                                                               ccTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAg               >  1:2595686/1‑148 (MQ=255)
                                                                                                                               ccTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAg               <  1:1427676/148‑1 (MQ=255)
                                                                                                                                cTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAgc              >  2:1447217/1‑148 (MQ=255)
                                                                                                                                       cgcATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCaa       >  2:2382258/1‑148 (MQ=255)
                                                                                                                                          aTTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTc    <  2:2401575/148‑1 (MQ=255)
                                                                                                                                            tttATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCa  >  2:2883670/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CCCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCA  >  CP009273/2021811‑2022098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGGCCGCCCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTA  >  CP009273/2021805‑2022102
                                                                                                                                                     |                                                                                                                                                    
GGGCCGCCCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTA                                                                                                                                                      <  SRR19973065.2831877/150‑1 (MQ=60)
 GGCCGCCCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTAT                                                                                                                                                     <  SRR19973065.2634970/150‑1 (MQ=60)
  GCCGCCCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGACGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATC                                                                                                                                                    <  SRR19973065.2166968/150‑1 (MQ=60)
   CCGCCCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCT                                                                                                                                                   <  SRR19973065.195849/150‑1 (MQ=60)
    CGCCCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTA                                                                                                                                                  >  SRR19973065.963386/1‑150 (MQ=60)
      CCCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACG                                                                                                                                                >  SRR19973065.2820875/1‑150 (MQ=60)
      CCCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACG                                                                                                                                                >  SRR19973065.1943043/1‑150 (MQ=60)
       CCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGG                                                                                                                                               >  SRR19973065.3002378/1‑150 (MQ=60)
       CCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGG                                                                                                                                               >  SRR19973065.554358/1‑150 (MQ=60)
          CTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTT                                                                                                                                            <  SRR19973065.934338/150‑1 (MQ=60)
          CTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTT                                                                                                                                            <  SRR19973065.780793/150‑1 (MQ=60)
           TTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTT                                                                                                                                           >  SRR19973065.2813827/1‑150 (MQ=60)
           TTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTT                                                                                                                                           >  SRR19973065.2813805/1‑150 (MQ=60)
           TTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTT                                                                                                                                           <  SRR19973065.2939320/150‑1 (MQ=60)
             TAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCA                                                                                                                                         >  SRR19973065.1486660/1‑150 (MQ=60)
               ACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACT                                                                                                                                       >  SRR19973065.98398/1‑150 (MQ=60)
                CCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTC                                                                                                                                      >  SRR19973065.781497/1‑150 (MQ=60)
                CCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTC                                                                                                                                      >  SRR19973065.781683/1‑150 (MQ=60)
                  GTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCC                                                                                                                                    >  SRR19973065.3517437/1‑150 (MQ=60)
                        TCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACC                                                                                                                              >  SRR19973065.2656840/1‑150 (MQ=60)
                          CTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCAC                                                                                                                            >  SRR19973065.1635065/1‑150 (MQ=60)
                            CACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGA                                                                                                                          >  SRR19973065.906438/1‑150 (MQ=60)
                             ACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGAT                                                                                                                         <  SRR19973065.3229258/150‑1 (MQ=60)
                                    ATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCG                                                                                                                  >  SRR19973065.1641505/1‑150 (MQ=60)
                                    ATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCG                                                                                                                  >  SRR19973065.2418557/1‑150 (MQ=60)
                                    ATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCG                                                                                                                  >  SRR19973065.2416675/1‑150 (MQ=60)
                                      AAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGAT                                                                                                                <  SRR19973065.2728374/150‑1 (MQ=60)
                                      AAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGAT                                                                                                                <  SRR19973065.382914/150‑1 (MQ=60)
                                      AAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGAT                                                                                                                <  SRR19973065.2164967/150‑1 (MQ=60)
                                      AAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGAT                                                                                                                >  SRR19973065.3515993/1‑150 (MQ=60)
                                       AAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATG                                                                                                               <  SRR19973065.2817431/150‑1 (MQ=60)
                                       AAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATG                                                                                                               <  SRR19973065.2817428/150‑1 (MQ=60)
                                       AAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATG                                                                                                               <  SRR19973065.66716/150‑1 (MQ=60)
                                        AGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCTGCCGATGA                                                                                                              <  SRR19973065.491389/150‑1 (MQ=60)
                                        AGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGA                                                                                                              >  SRR19973065.2293525/1‑150 (MQ=60)
                                         GCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGAT                                                                                                             >  SRR19973065.1189366/1‑150 (MQ=60)
                                          CTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATA                                                                                                            <  SRR19973065.1114193/150‑1 (MQ=60)
                                           TCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAA                                                                                                           >  SRR19973065.1957815/1‑150 (MQ=60)
                                            CATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAA                                                                                                          >  SRR19973065.1489516/1‑150 (MQ=60)
                                            CATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGAGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATTCCGCCGATGATAAA                                                                                                          >  SRR19973065.2134937/1‑150 (MQ=60)
                                             ATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAAT                                                                                                         >  SRR19973065.2113776/1‑150 (MQ=60)
                                              TACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATC                                                                                                        >  SRR19973065.279210/1‑150 (MQ=60)
                                              TACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATC                                                                                                        <  SRR19973065.836896/150‑1 (MQ=60)
                                              TACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATC                                                                                                        >  SRR19973065.3114548/1‑150 (MQ=60)
                                               ACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCC                                                                                                       >  SRR19973065.388900/1‑150 (MQ=60)
                                               ACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCC                                                                                                       <  SRR19973065.3261977/150‑1 (MQ=60)
                                               ACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCC                                                                                                       >  SRR19973065.1554285/1‑150 (MQ=60)
                                                  CGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAG                                                                                                    >  SRR19973065.3202220/1‑150 (MQ=60)
                                                   GCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGA                                                                                                   <  SRR19973065.259732/150‑1 (MQ=60)
                                                   GCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGA                                                                                                   >  SRR19973065.3026019/1‑150 (MQ=60)
                                                      TCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATC                                                                                                >  SRR19973065.379506/1‑150 (MQ=60)
                                                       CCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCA                                                                                               >  SRR19973065.3435881/1‑150 (MQ=60)
                                                          TTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGAT                                                                                            <  SRR19973065.1354043/150‑1 (MQ=60)
                                                          TTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGAT                                                                                            <  SRR19973065.3396875/150‑1 (MQ=60)
                                                               CGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAA                                                                                       <  SRR19973065.3130748/150‑1 (MQ=60)
                                                               CGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAA                                                                                       >  SRR19973065.98695/1‑150 (MQ=60)
                                                                   TGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAG                                                                                   <  SRR19973065.2206235/150‑1 (MQ=60)
                                                                      AACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCG                                                                                <  SRR19973065.1045601/150‑1 (MQ=60)
                                                                      AACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCG                                                                                <  SRR19973065.2110466/150‑1 (MQ=60)
                                                                      AACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCG                                                                                >  SRR19973065.2574179/1‑150 (MQ=60)
                                                                          TGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAA                                                                            >  SRR19973065.681570/1‑150 (MQ=60)
                                                                           GTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTTCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAAT                                                                           <  SRR19973065.3025772/150‑1 (MQ=60)
                                                                              CACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATC                                                                        >  SRR19973065.271062/1‑150 (MQ=60)
                                                                              CACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATC                                                                        >  SRR19973065.2535489/1‑150 (MQ=60)
                                                                                 CTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATC                                                                     <  SRR19973065.1888988/150‑1 (MQ=60)
                                                                                  TTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCG                                                                    <  SRR19973065.743956/150‑1 (MQ=60)
                                                                                    AGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCC                                                                  <  SRR19973065.1374601/150‑1 (MQ=60)
                                                                                     GTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCA                                                                 <  SRR19973065.2258042/150‑1 (MQ=60)
                                                                                         AAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTAC                                                             >  SRR19973065.1881404/1‑150 (MQ=60)
                                                                                          AGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCTATTATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACT                                                            <  SRR19973065.3133680/150‑1 (MQ=60)
                                                                                          AGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACT                                                            <  SRR19973065.3133715/150‑1 (MQ=60)
                                                                                          AGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACT                                                            <  SRR19973065.801304/150‑1 (MQ=60)
                                                                                            ATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGC                                                          >  SRR19973065.825218/1‑150 (MQ=60)
                                                                                                  AATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTG                                                    >  SRR19973065.3200018/1‑150 (MQ=60)
                                                                                                   ATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGC                                                   <  SRR19973065.1849565/150‑1 (MQ=60)
                                                                                                    TTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCT                                                  <  SRR19973065.3265155/150‑1 (MQ=60)
                                                                                                        GCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCC                                              <  SRR19973065.2640243/150‑1 (MQ=60)
                                                                                                               AGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAA                                       >  SRR19973065.1016160/1‑150 (MQ=60)
                                                                                                                   AGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTT                                   >  SRR19973065.1023729/1‑150 (MQ=60)
                                                                                                                   AGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTT                                   >  SRR19973065.185565/1‑150 (MQ=60)
                                                                                                                    GTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTC                                  <  SRR19973065.381161/150‑1 (MQ=60)
                                                                                                                       CGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAAT                               <  SRR19973065.252571/150‑1 (MQ=60)
                                                                                                                          GTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGC                            <  SRR19973065.681879/150‑1 (MQ=60)
                                                                                                                           TATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCG                           <  SRR19973065.2368600/150‑1 (MQ=60)
                                                                                                                           TATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCG                           <  SRR19973065.1540412/150‑1 (MQ=60)
                                                                                                                           TATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCG                           >  SRR19973065.421682/1‑150 (MQ=60)
                                                                                                                            ATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGT                          >  SRR19973065.1533263/1‑150 (MQ=60)
                                                                                                                              TAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGA                        >  SRR19973065.293199/1‑150 (MQ=60)
                                                                                                                              TAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGA                        >  SRR19973065.110408/1‑150 (MQ=60)
                                                                                                                              TAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGA                        >  SRR19973065.292096/1‑150 (MQ=60)
                                                                                                                               AATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGAT                       >  SRR19973065.2536124/1‑150 (MQ=60)
                                                                                                                                  GTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCA                    <  SRR19973065.2527247/150‑1 (MQ=60)
                                                                                                                                  GTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCA                    <  SRR19973065.2527351/150‑1 (MQ=60)
                                                                                                                                   TGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAG                   >  SRR19973065.1920597/1‑150 (MQ=60)
                                                                                                                                   TGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAG                   >  SRR19973065.2611822/1‑150 (MQ=60)
                                                                                                                                     CCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCG                 <  SRR19973065.1436972/150‑1 (MQ=60)
                                                                                                                                       TGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGC               >  SRR19973065.2491157/1‑150 (MQ=60)
                                                                                                                                         ACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGC             >  SRR19973065.3121239/1‑150 (MQ=60)
                                                                                                                                                 TTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCAT     <  SRR19973065.236502/150‑1 (MQ=60)
                                                                                                                                                    TATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTA  <  SRR19973065.2409993/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GGGCCGCCCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTA  >  CP009273/2021805‑2022102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑