Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,425,944 C→A 41.6% D160Y (GAC→TAC)  folC ← bifunctional folylpolyglutamate synthase/ dihydrofolate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,425,9440CA41.6% 185.7 / 188.7 175D160Y (GAC→TAC) folCbifunctional folylpolyglutamate synthase/ dihydrofolate synthase
Reads supporting (aligned to +/- strand):  ref base C (58/44);  new base A (34/39);  total (92/83)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

GGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAATCTC  >  CP009273/2425798‑2426091
                                                                                                                                                  |                                                                                                                                                   
   aGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACa                                                                                                                                                 >  1:2477781/1‑148 (MQ=255)
   aGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACa                                                                                                                                                 >  1:235218/1‑148 (MQ=255)
   aGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACa                                                                                                                                                 >  1:1422912/1‑148 (MQ=255)
     aaGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAat                                                                                                                                               <  2:1169101/148‑1 (MQ=255)
     aaGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAat                                                                                                                                               <  1:673608/148‑1 (MQ=255)
        gCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATAtt                                                                                                                                            >  1:995524/1‑148 (MQ=255)
        gCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATAtt                                                                                                                                            >  1:2063135/1‑148 (MQ=255)
         cATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTg                                                                                                                                           <  1:2267159/148‑1 (MQ=255)
          aTTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTgg                                                                                                                                          <  2:2511721/148‑1 (MQ=255)
          aTTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTgg                                                                                                                                          <  2:1222784/148‑1 (MQ=255)
           tttCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGt                                                                                                                                         <  2:739437/148‑1 (MQ=255)
           tttCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGt                                                                                                                                         <  2:739506/148‑1 (MQ=255)
            ttCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGtt                                                                                                                                        >  2:2287179/1‑148 (MQ=255)
                 gCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTc                                                                                                                                   <  1:295609/148‑1 (MQ=255)
                       cGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAgacg                                                                                                                             <  1:1927891/148‑1 (MQ=255)
                        gACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAgacga                                                                                                                            >  2:451563/1‑148 (MQ=255)
                        gACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAgacga                                                                                                                            <  2:56417/148‑1 (MQ=255)
                         aCAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAgacgac                                                                                                                           >  2:1585205/1‑148 (MQ=255)
                         aCAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAgacgac                                                                                                                           >  2:513863/1‑148 (MQ=255)
                          cAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGAcc                                                                                                                          >  1:2191176/1‑148 (MQ=255)
                                  ggTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGcc                                                                                                                  >  1:1074818/1‑148 (MQ=255)
                                    tttttCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCt                                                                                                                >  2:231163/1‑148 (MQ=255)
                                        tCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACtt                                                                                                            <  2:3070906/148‑1 (MQ=255)
                                         cGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTc                                                                                                           <  1:652896/148‑1 (MQ=255)
                                         cGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTc                                                                                                           <  1:3119225/148‑1 (MQ=255)
                                                 aaGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATca                                                                                                   <  2:1585266/148‑1 (MQ=255)
                                                   gATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATcacc                                                                                                 <  2:326258/148‑1 (MQ=255)
                                                   gATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATcacc                                                                                                 >  1:3472329/1‑148 (MQ=255)
                                                   gATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATcacc                                                                                                 >  1:714498/1‑148 (MQ=255)
                                                    aTGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATcacca                                                                                                >  2:365593/1‑148 (MQ=255)
                                                    aTGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATcacca                                                                                                >  2:150886/1‑148 (MQ=255)
                                                       ccTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGt                                                                                             <  1:1143600/148‑1 (MQ=255)
                                                         tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCa                                                                                           <  1:1334667/148‑1 (MQ=255)
                                                            tttCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGt                                                                                        >  1:1786291/1‑148 (MQ=255)
                                                            tttCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGt                                                                                        >  1:1786095/1‑148 (MQ=255)
                                                              tctcGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTtg                                                                                      >  1:1596281/1‑148 (MQ=255)
                                                              tctcGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTtg                                                                                      >  2:1849024/1‑148 (MQ=255)
                                                              tctcGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTtg                                                                                      >  2:1848788/1‑148 (MQ=255)
                                                               ctcGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTtgt                                                                                     <  2:166482/148‑1 (MQ=255)
                                                                     aCCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGc                                                                               <  2:2783389/148‑1 (MQ=255)
                                                                     aCCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGc                                                                               <  2:1005412/148‑1 (MQ=255)
                                                                      ccAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCt                                                                              >  2:2172898/1‑148 (MQ=255)
                                                                       cAATACTTTCGCGATCTGGACCCAGCAAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCtt                                                                             >  2:2505339/1‑148 (MQ=255)
                                                                       cAATACTTTCGCGATCTGGACCCAGCAAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCtt                                                                             >  2:49729/1‑148 (MQ=255)
                                                                         aTACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACTGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGa                                                                           <  2:3298515/148‑1 (MQ=255)
                                                                         aTACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACTGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGa                                                                           <  2:493128/148‑1 (MQ=255)
                                                                          tACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGaa                                                                          >  2:2763262/1‑148 (MQ=255)
                                                                          tACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGaa                                                                          >  2:279524/1‑148 (MQ=255)
                                                                          tACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGaa                                                                          <  1:424810/148‑1 (MQ=255)
                                                                             tttCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAg                                                                       >  1:665677/1‑148 (MQ=255)
                                                                              ttCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGc                                                                      >  1:1304935/1‑148 (MQ=255)
                                                                               tCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGcc                                                                     >  1:3310310/1‑148 (MQ=255)
                                                                               tCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGcc                                                                     >  1:3310439/1‑148 (MQ=255)
                                                                               tCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGcc                                                                     <  1:2715881/148‑1 (MQ=255)
                                                                                   gATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACaa                                                                 <  1:3312697/148‑1 (MQ=255)
                                                                                   gATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACaa                                                                 <  2:2977799/148‑1 (MQ=255)
                                                                                        ggACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCg                                                            <  2:2015521/148‑1 (MQ=255)
                                                                                          aCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGAc                                                          <  2:2490810/148‑1 (MQ=255)
                                                                                          aCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGAc                                                          >  1:2389697/1‑148 (MQ=255)
                                                                                            ccagccagTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAg                                                        <  1:1426549/148‑1 (MQ=255)
                                                                                                    tCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGcgtaccgt                                                >  2:3257757/1‑148 (MQ=255)
                                                                                                      ggTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGcgtaccgtac                                              <  2:157311/148‑1 (MQ=255)
                                                                                                      ggTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGcgtaccgtac                                              <  2:2231129/148‑1 (MQ=255)
                                                                                                       gTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACt                                             <  2:835346/148‑1 (MQ=255)
                                                                                                         aTGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCg                                           >  1:1818034/1‑148 (MQ=255)
                                                                                                         aTGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCg                                           <  1:529628/148‑1 (MQ=255)
                                                                                                          tGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGa                                          >  2:2099101/1‑148 (MQ=255)
                                                                                                           gATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGaa                                         <  2:2433318/148‑1 (MQ=255)
                                                                                                            aTCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGaaa                                        >  1:909805/1‑148 (MQ=255)
                                                                                                            aTCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGaaa                                        <  2:3264875/148‑1 (MQ=255)
                                                                                                               cAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAAGATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAg                                     <  2:2343268/148‑1 (MQ=255)
                                                                                                                 gcgcAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGt                                   >  1:2510336/1‑148 (MQ=255)
                                                                                                                 gcgcAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGt                                   >  1:2510369/1‑148 (MQ=255)
                                                                                                                 gcgcAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGt                                   <  2:961218/148‑1 (MQ=255)
                                                                                                                     aaTACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAgg                               <  2:2070122/148‑1 (MQ=255)
                                                                                                                        aCTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGaa                            <  1:596269/148‑1 (MQ=255)
                                                                                                                        aCTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGaa                            <  2:1422912/148‑1 (MQ=255)
                                                                                                                          tGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAat                          >  2:1577362/1‑148 (MQ=255)
                                                                                                                            gTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAatat                        >  2:2754046/1‑148 (MQ=255)
                                                                                                                            gTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAatat                        >  1:944263/1‑148 (MQ=255)
                                                                                                                                ctacCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCAcc                    >  1:3543203/1‑148 (MQ=255)
                                                                                                                                 tacCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACcg                   >  1:2669555/1‑148 (MQ=255)
                                                                                                                                  acCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACcgc                  >  1:1024235/1‑148 (MQ=255)
                                                                                                                                  acCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACcgc                  >  1:1024225/1‑148 (MQ=255)
                                                                                                                                   ccGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACcgcg                 >  2:1079342/1‑148 (MQ=255)
                                                                                                                                     gcgACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTg               >  2:122272/1‑148 (MQ=255)
                                                                                                                                        aCATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCg            >  1:777913/1‑148 (MQ=255)
                                                                                                                                        aCATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCg            <  2:2108404/148‑1 (MQ=255)
                                                                                                                                         cATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGa           <  1:2851746/148‑1 (MQ=255)
                                                                                                                                              gCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCaa      >  2:3530812/1‑148 (MQ=255)
                                                                                                                                              gCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCaa      >  2:791298/1‑148 (MQ=255)
                                                                                                                                               cGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAAt     >  2:3380551/1‑148 (MQ=255)
                                                                                                                                               cGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAAt     >  2:3380613/1‑148 (MQ=255)
                                                                                                                                               cGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAAt     >  2:3382175/1‑148 (MQ=255)
                                                                                                                                               cGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACGGCGTGCCGATTCAAt     <  1:2052166/148‑1 (MQ=255)
                                                                                                                                                gTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAAtc    <  2:2738100/148‑1 (MQ=255)
                                                                                                                                                 tCGACAATATTGTTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAAtct   >  1:243496/1‑148 (MQ=255)
                                                                                                                                                 tCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAAtct   >  1:2748047/1‑148 (MQ=255)
                                                                                                                                                 tCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAAtct   >  1:243270/1‑148 (MQ=255)
                                                                                                                                                  cGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAAtctc  <  2:1086658/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAATCTC  >  CP009273/2425798‑2426091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A