Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,425,944 C→A 20.9% D160Y (GAC→TAC)  folC ← bifunctional folylpolyglutamate synthase/ dihydrofolate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,425,9440CA20.9% 395.3 / 70.1 153D160Y (GAC→TAC) folCbifunctional folylpolyglutamate synthase/ dihydrofolate synthase
Reads supporting (aligned to +/- strand):  ref base C (56/65);  new base A (16/16);  total (72/81)
Fisher's exact test for biased strand distribution p-value = 8.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01

Only 100 of 154 total aligned reads displayed.

GTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAATCTC  >  CP009273/2425799‑2426091
                                                                                                                                                 |                                                                                                                                                   
gTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGa                                                                                                                                                   <  2:3401443/148‑1 (MQ=255)
  aGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACa                                                                                                                                                 <  2:3469351/148‑1 (MQ=255)
     aGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAata                                                                                                                                              <  1:3458363/148‑1 (MQ=255)
      ggCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAatat                                                                                                                                             >  1:2186103/1‑148 (MQ=255)
          tttCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGt                                                                                                                                         >  2:2812551/1‑148 (MQ=255)
          tttCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGt                                                                                                                                         <  1:1683736/148‑1 (MQ=255)
          tttCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGt                                                                                                                                         >  1:1705537/1‑148 (MQ=255)
              cGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCg                                                                                                                                     >  2:868159/1‑148 (MQ=255)
                gCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTc                                                                                                                                   >  1:752967/1‑148 (MQ=255)
                gCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTc                                                                                                                                   <  1:1800664/148‑1 (MQ=255)
                 cTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTcc                                                                                                                                  <  2:1596340/148‑1 (MQ=255)
                   cACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAg                                                                                                                                >  1:1779381/1‑148 (MQ=255)
                    aCCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAga                                                                                                                               >  1:3240308/1‑148 (MQ=255)
                       gACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAgacga                                                                                                                            <  1:960881/148‑1 (MQ=255)
                         cAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGAcc                                                                                                                          >  2:1852908/1‑148 (MQ=255)
                             tGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGccc                                                                                                                      <  2:1677140/148‑1 (MQ=255)
                             tGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGccc                                                                                                                      >  1:2579726/1‑148 (MQ=255)
                                cGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGc                                                                                                                   >  1:2640158/1‑148 (MQ=255)
                                 ggTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGcc                                                                                                                  <  2:2326076/148‑1 (MQ=255)
                                 ggTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGcc                                                                                                                  <  1:2923165/148‑1 (MQ=255)
                                 ggTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGcc                                                                                                                  <  1:2920863/148‑1 (MQ=255)
                                  gTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGccc                                                                                                                 <  1:1570796/148‑1 (MQ=255)
                                  gTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGccc                                                                                                                 <  1:3341630/148‑1 (MQ=255)
                                         gCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTcc                                                                                                          <  1:2521988/148‑1 (MQ=255)
                                         gCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTcc                                                                                                          <  2:1727358/148‑1 (MQ=255)
                                            gCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGa                                                                                                       <  2:935189/148‑1 (MQ=255)
                                             cGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGaa                                                                                                      <  2:2848321/148‑1 (MQ=255)
                                             cGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGaa                                                                                                      <  1:1125594/148‑1 (MQ=255)
                                              ggAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAAt                                                                                                     <  1:3483856/148‑1 (MQ=255)
                                               gAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATc                                                                                                    >  2:69458/1‑148 (MQ=255)
                                                aaGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATca                                                                                                   >  1:2674398/1‑148 (MQ=255)
                                                  gATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATcacc                                                                                                 >  2:2432518/1‑148 (MQ=255)
                                                  gATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATcacc                                                                                                 <  2:2035515/148‑1 (MQ=255)
                                                  gATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATcacc                                                                                                 <  2:1664998/148‑1 (MQ=255)
                                                  gATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATcacc                                                                                                 <  2:2637091/148‑1 (MQ=255)
                                                         gCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCaa                                                                                          >  2:1051583/1‑148 (MQ=255)
                                                          cTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAg                                                                                         <  1:2253480/148‑1 (MQ=255)
                                                            ttCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGtt                                                                                       >  1:2936439/1‑148 (MQ=255)
                                                                    aCCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGc                                                                               <  2:2310372/148‑1 (MQ=255)
                                                                    aCCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGc                                                                               >  2:3490759/1‑148 (MQ=255)
                                                                     ccAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCt                                                                              <  1:424234/148‑1 (MQ=255)
                                                                        aTACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGa                                                                           >  2:1952011/1‑148 (MQ=255)
                                                                         tACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGaa                                                                          >  2:784965/1‑148 (MQ=255)
                                                                          aCTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAAc                                                                         >  2:3530197/1‑148 (MQ=255)
                                                                          aCTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAAc                                                                         >  1:1119014/1‑148 (MQ=255)
                                                                                gcgATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCcac                                                                   >  2:3373820/1‑148 (MQ=255)
                                                                                 cgATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCcaca                                                                  >  1:1746384/1‑148 (MQ=255)
                                                                                    tCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACg                                                               >  2:523859/1‑148 (MQ=255)
                                                                                    tCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACGACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACg                                                               <  2:2943033/148‑1 (MQ=255)
                                                                                     cTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGc                                                              >  2:3449424/1‑148 (MQ=255)
                                                                                      tGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGcc                                                             >  2:3064067/1‑148 (MQ=255)
                                                                                         aCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGAc                                                          <  1:2095459/148‑1 (MQ=255)
                                                                                         aCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACCTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGAc                                                          <  1:2095370/148‑1 (MQ=255)
                                                                                         aCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGAc                                                          >  1:1408576/1‑148 (MQ=255)
                                                                                          cccAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACa                                                         >  2:2442916/1‑148 (MQ=255)
                                                                                           ccagccagTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAg                                                        >  1:2968296/1‑148 (MQ=255)
                                                                                             agccagTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGcg                                                      <  1:1604407/148‑1 (MQ=255)
                                                                                             agccagTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGcg                                                      <  1:3363279/148‑1 (MQ=255)
                                                                                               ccagTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGcgta                                                    >  1:1316433/1‑148 (MQ=255)
                                                                                               ccagTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGcgta                                                    <  1:3057430/148‑1 (MQ=255)
                                                                                               ccagTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGcgta                                                    <  1:2505247/148‑1 (MQ=255)
                                                                                                       tATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTc                                            >  2:2627818/1‑148 (MQ=255)
                                                                                                          gATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGaa                                         >  1:872916/1‑148 (MQ=255)
                                                                                                          gATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGaa                                         <  1:614131/148‑1 (MQ=255)
                                                                                                          gATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGaa                                         <  1:613956/148‑1 (MQ=255)
                                                                                                           aTCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGaaa                                        >  1:1227505/1‑148 (MQ=255)
                                                                                                           aTCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGaaa                                        >  1:1228648/1‑148 (MQ=255)
                                                                                                              cAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAg                                     >  1:524200/1‑148 (MQ=255)
                                                                                                               aGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAgg                                    <  2:3113258/148‑1 (MQ=255)
                                                                                                               aGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAgg                                    <  1:1134843/148‑1 (MQ=255)
                                                                                                                gcgcAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGt                                   >  1:2681428/1‑148 (MQ=255)
                                                                                                                gcgcAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGt                                   >  1:2808089/1‑148 (MQ=255)
                                                                                                                gcgcAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGt                                   >  1:2808413/1‑148 (MQ=255)
                                                                                                                 cgcAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAgg                                    >  1:3091301/1‑146 (MQ=255)
                                                                                                                 cgcAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAgg                                    >  1:3091371/1‑146 (MQ=255)
                                                                                                                 cgcAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAgg                                    >  1:3139091/1‑146 (MQ=255)
                                                                                                                 cgcAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAgg                                    <  2:3139181/146‑1 (MQ=255)
                                                                                                                 cgcAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAgg                                    <  2:3139091/146‑1 (MQ=255)
                                                                                                                 cgcAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAgg                                    <  2:3091371/146‑1 (MQ=255)
                                                                                                                 cgcAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTc                                  <  1:784965/148‑1 (MQ=255)
                                                                                                                  gcAATACTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCa                                 <  2:2622689/148‑1 (MQ=255)
                                                                                                                   cAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAg                                <  1:2591067/148‑1 (MQ=255)
                                                                                                                       aCTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGaa                            <  2:1316310/148‑1 (MQ=255)
                                                                                                                        cTGGTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGaaa                           <  2:2579726/148‑1 (MQ=255)
                                                                                                                         tGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAat                          >  2:1078569/1‑148 (MQ=255)
                                                                                                                         tGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAat                          <  2:2877929/148‑1 (MQ=255)
                                                                                                                         tGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAat                          >  1:2371161/1‑148 (MQ=255)
                                                                                                                         tGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAat                          >  1:2371468/1‑148 (MQ=255)
                                                                                                                           gTTACTACCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAatat                        >  2:1310465/1‑148 (MQ=255)
                                                                                                                             tactacCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCa                      >  2:126874/1‑148 (MQ=255)
                                                                                                                             tactacCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCa                      >  2:2063013/1‑148 (MQ=255)
                                                                                                                             tactacCGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCa                      >  2:437320/1‑148 (MQ=255)
                                                                                                                                  ccGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCGGACAGCGTACCGTACGCGAAATAGGTCAGGGAAATATCACcgcg                 <  2:3428237/148‑1 (MQ=255)
                                                                                                                                  ccGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACcgcg                 <  2:1745996/148‑1 (MQ=255)
                                                                                                                                  ccGCGACATCGGCGTAGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACcgcg                 <  2:1427353/148‑1 (MQ=255)
                                                                                                                                        cATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGa           <  1:1112012/148‑1 (MQ=255)
                                                                                                                                             gCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCaa      <  2:652319/148‑1 (MQ=255)
                                                                                                                                             gCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCaa      <  2:652305/148‑1 (MQ=255)
                                                                                                                                              cGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAAt     <  2:871804/148‑1 (MQ=255)
                                                                                                                                                 cGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAAtctc  >  2:1754116/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAATCTC  >  CP009273/2425799‑2426091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A