Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,426,731 G→T 21.0% I225I (ATC→ATA)  accD ← acetyl‑CoA carboxylase, beta (carboxyltransferase) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,426,7310GT21.0% 902.8 / 176.6 331I225I (ATC→ATA) accDacetyl‑CoA carboxylase, beta (carboxyltransferase) subunit
Reads supporting (aligned to +/- strand):  ref base G (141/120);  new base T (35/34);  total (176/155)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 335 total aligned reads displayed.

GCCAGAATGCTCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGCCGCAGAGGTTTTCGCCATCTG  >  CP009273/2426582‑2426880
                                                                                                                                                     |                                                                                                                                                     
  cAGAATGCTCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGAt                                                                                                                                                     >  2:2801862/1‑150 (MQ=255)
   aGAATGCTCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGAtt                                                                                                                                                    >  2:627099/1‑150 (MQ=255)
   aGAATGCTCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGAtt                                                                                                                                                    >  2:3316708/1‑150 (MQ=255)
    gAATGCTCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTa                                                                                                                                                   <  2:1315287/150‑1 (MQ=255)
    gAATGCTCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTa                                                                                                                                                   <  2:239941/150‑1 (MQ=255)
    gAATGCTCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACAAGCGGACCGGCAAAGCCGATTa                                                                                                                                                   >  1:3465155/1‑150 (MQ=255)
     aaTGCTCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTaa                                                                                                                                                  <  1:1540783/150‑1 (MQ=255)
      aTGCTCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAAc                                                                                                                                                 >  1:124478/1‑150 (MQ=255)
         cTCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCt                                                                                                                                              <  2:1155868/150‑1 (MQ=255)
          tCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCtt                                                                                                                                             <  2:2211448/150‑1 (MQ=255)
           cGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCttt                                                                                                                                            >  2:1553010/1‑150 (MQ=255)
           cGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCttt                                                                                                                                            <  1:3062001/150‑1 (MQ=255)
           cGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCttt                                                                                                                                            >  2:3962090/1‑150 (MQ=255)
             ccAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCTATTAACGCTTTCg                                                                                                                                          <  1:867898/150‑1 (MQ=255)
              cAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCgg                                                                                                                                         >  1:3422239/1‑150 (MQ=255)
               aGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGt                                                                                                                                        <  1:3855774/150‑1 (MQ=255)
                gTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGtt                                                                                                                                       >  1:4056153/1‑150 (MQ=255)
                 tttCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTc                                                                                                                                      >  2:524759/1‑150 (MQ=255)
                  ttCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCa                                                                                                                                     >  1:359461/1‑150 (MQ=255)
                   tCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAg                                                                                                                                    >  2:3870117/1‑150 (MQ=255)
                   tCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAg                                                                                                                                    >  1:3235998/1‑150 (MQ=255)
                     aGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCg                                                                                                                                  >  1:1844482/1‑150 (MQ=255)
                           aTTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTg                                                                                                                            <  1:1222872/150‑1 (MQ=255)
                           aTTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTg                                                                                                                            >  2:3530878/1‑150 (MQ=255)
                             ttCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTgag                                                                                                                          <  1:4725623/150‑1 (MQ=255)
                             ttCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTgag                                                                                                                          >  2:3399670/1‑150 (MQ=255)
                                cggacgacggacgaTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATc                                                                                                                       >  2:2165903/1‑150 (MQ=255)
                                 ggacgacggacgaTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCTATTAACGCTTTCGGTTCAGCGATGTTGAGATCg                                                                                                                      <  1:2297407/150‑1 (MQ=255)
                                 ggacgacggacgaTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCg                                                                                                                      >  2:4242900/1‑150 (MQ=255)
                                  gacgacggacgaTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGc                                                                                                                     >  1:2189713/1‑150 (MQ=255)
                                  gacgacggacgaTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGc                                                                                                                     >  1:4666607/1‑150 (MQ=255)
                                  gacgacggacgaTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGc                                                                                                                     >  2:3628987/1‑150 (MQ=255)
                                     gacggacgaTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCTATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCa                                                                                                                  <  2:3830680/150‑1 (MQ=255)
                                       cggacgaTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTGTCGGTTCAGCGAGGTTGAGATCGCCCAGc                                                                                                                >  2:3187163/1‑150 (MQ=255)
                                            gaTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGc                                                                                                           <  2:3763068/150‑1 (MQ=255)
                                             aTCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCTATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCg                                                                                                          >  2:4284029/1‑150 (MQ=255)
                                              tCATGTCGATCGCTCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCTGACCGGCAAAGACAATTAACGCTTTCGGTTCATCGATTTTGAGATCGCCCAGCATGGCGa                                                                                                         <  1:1002434/150‑1 (MQ=255)
                                               cATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCTATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGaa                                                                                                        >  1:588837/1‑150 (MQ=255)
                                                   tCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACtt                                                                                                    >  1:4207945/1‑150 (MQ=255)
                                                   tCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACtt                                                                                                    >  1:4294104/1‑150 (MQ=255)
                                                   tCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACtt                                                                                                    >  1:51719/1‑150 (MQ=255)
                                                       tCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAg                                                                                                <  2:2437308/150‑1 (MQ=255)
                                                       tCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAg                                                                                                <  2:4779190/150‑1 (MQ=255)
                                                        cgcgCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCTATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGa                                                                                               >  1:793987/1‑150 (MQ=255)
                                                             cTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAacac                                                                                          >  1:2122160/1‑150 (MQ=255)
                                                              tttCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCTATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACAcc                                                                                         <  2:755138/150‑1 (MQ=255)
                                                              tttCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACAcc                                                                                         >  1:4626835/1‑150 (MQ=255)
                                                               ttCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCg                                                                                        >  1:3896287/1‑150 (MQ=255)
                                                                 ctcGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGcc                                                                                      >  1:2889979/1‑150 (MQ=255)
                                                                     aTCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCAtc                                                                                  <  1:4745408/150‑1 (MQ=255)
                                                                      tCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCAtcg                                                                                 >  1:3637785/1‑150 (MQ=255)
                                                                        aGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCAtcgtc                                                                               <  1:2999124/150‑1 (MQ=255)
                                                                          gAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCgtcgg                                                                             >  2:2358696/1‑150 (MQ=255)
                                                                              tCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCgtcgggtcg                                                                         <  1:1934447/150‑1 (MQ=255)
                                                                               cACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCTATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCgtcgggtcgg                                                                        >  1:2514879/1‑150 (MQ=255)
                                                                               cACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCgtcgggtcgg                                                                        <  2:941025/150‑1 (MQ=255)
                                                                               cACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCgtcgggtcgg                                                                        >  1:3258771/1‑150 (MQ=255)
                                                                                 cTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGggtcggtc                                                                      >  1:3127498/1‑150 (MQ=255)
                                                                                  tGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCa                                                                     <  1:4537882/150‑1 (MQ=255)
                                                                                     gcTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCa                                                                  >  2:4409312/1‑150 (MQ=255)
                                                                                      cTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCTATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCAc                                                                 >  1:2250456/1‑150 (MQ=255)
                                                                                      cTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCAc                                                                 <  1:2805798/150‑1 (MQ=255)
                                                                                       tGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACg                                                                <  2:4194925/150‑1 (MQ=255)
                                                                                          aaTCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCTATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGgag                                                             <  1:2455935/150‑1 (MQ=255)
                                                                                            tCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGAt                                                           <  1:1655258/150‑1 (MQ=255)
                                                                                             ccAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCTATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATg                                                          <  2:2909551/150‑1 (MQ=255)
                                                                                             ccAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATg                                                          <  1:3316761/150‑1 (MQ=255)
                                                                                              cAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGt                                                         >  1:984874/1‑150 (MQ=255)
                                                                                               aGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTa                                                        >  2:1767246/1‑150 (MQ=255)
                                                                                                ggcggcAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTAc                                                       >  2:4319515/1‑150 (MQ=255)
                                                                                                ggcggcAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTAc                                                       >  2:570211/1‑150 (MQ=255)
                                                                                                ggcggcAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTAc                                                       <  2:3245905/150‑1 (MQ=255)
                                                                                                 gcggcAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACg                                                      <  1:732475/150‑1 (MQ=255)
                                                                                                  cggcAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACgg                                                     >  1:1173236/1‑150 (MQ=255)
                                                                                                   ggcAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGc                                                    >  2:2661928/1‑150 (MQ=255)
                                                                                                     cAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCTATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCaa                                                  <  2:4684155/150‑1 (MQ=255)
                                                                                                     cAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCaa                                                  <  2:61081/150‑1 (MQ=255)
                                                                                                        tttttCGCGAACGGTCTGTTCGATCACTCGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGcc                                               <  2:563894/150‑1 (MQ=255)
                                                                                                        tttttCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCTATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGcc                                               <  1:2517558/150‑1 (MQ=255)
                                                                                                        tttttCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGcc                                               <  2:1427785/150‑1 (MQ=255)
                                                                                                                gAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCt                                       >  1:2615881/1‑150 (MQ=255)
                                                                                                                  aCGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGc                                     <  1:2380812/150‑1 (MQ=255)
                                                                                                                       cTGTTCGATAACACGCGGACCGGCAAAGCCTATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCAtttt                                >  1:2461768/1‑150 (MQ=255)
                                                                                                                       cTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCAtttt                                >  2:4555981/1‑150 (MQ=255)
                                                                                                                            cGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCa                           <  2:1844482/150‑1 (MQ=255)
                                                                                                                            cGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCAATTTTGCCa                           <  1:1095862/150‑1 (MQ=255)
                                                                                                                              aTAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGt                         >  2:198924/1‑150 (MQ=255)
                                                                                                                                aaCACGCGGACCGGCAAAGCCTATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTAGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGc                       >  2:1466466/1‑150 (MQ=255)
                                                                                                                                aaCACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGc                       >  1:1385478/1‑150 (MQ=255)
                                                                                                                                  cacGCGGACCGGCAAAGCCTATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGCCg                     <  1:2791751/150‑1 (MQ=255)
                                                                                                                                       ggACCGGCAAAGCCTATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGCCGCagag                >  2:3040080/1‑150 (MQ=255)
                                                                                                                                       ggACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGCTCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGCCGCagag                >  1:200905/1‑150 (MQ=255)
                                                                                                                                       ggACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGCCGCagag                <  2:1233965/150‑1 (MQ=255)
                                                                                                                                           cGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGCCGCAGAGGttt            >  2:4646678/1‑150 (MQ=255)
                                                                                                                                           cGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGCCGCAGAGGttt            <  2:2655954/150‑1 (MQ=255)
                                                                                                                                             gCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGCCGCAGAGGTTTTc          <  1:4350709/150‑1 (MQ=255)
                                                                                                                                              cAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGCCGCAGAGGTTTTCg         >  1:4729273/1‑150 (MQ=255)
                                                                                                                                                aaGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGCCGCAGAGGTTTTCGcc       >  2:3918763/1‑150 (MQ=255)
                                                                                                                                                     gATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGCCGCAGAGGTTTTCGCCATCTg  >  2:1388842/1‑150 (MQ=255)
                                                                                                                                                     gATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGCCGCAGAGGTTTTCGCCATCTg  <  2:858769/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCAGAATGCTCGCCAGTTTCAGGCGCATTTCCGGACGACGGACGATCATGTCGATCGCGCCTTTCTCGATCAGGAATTCACTGCGCTGGAATCCAGGCGGCAGTTTTTCGCGAACGGTCTGTTCGATAACACGCGGACCGGCAAAGCCGATTAACGCTTTCGGTTCAGCGATGTTGAGATCGCCCAGCATGGCGAAACTTGCAGAAACACCGCCCATCGTCGGGTCGGTCAGCACGGAGATGTACGGCAAGCCGCGCTCCTGCATTTTTGCCAGTGCCGCAGAGGTTTTCGCCATCTG  >  CP009273/2426582‑2426880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A