Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,047,301 G→T 39.6% T300N (ACC→AAC)  pepP ← proline aminopeptidase P II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,047,3010GT39.6% 248.9 / 213.9 207T300N (ACC→AAC) pepPproline aminopeptidase P II
Reads supporting (aligned to +/- strand):  ref base G (60/65);  new base T (41/41);  total (101/106)
Fisher's exact test for biased strand distribution p-value = 8.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 213 total aligned reads displayed.

AGAAAGGACGATGGGCGTTCTGAGCGATCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCCGCGTCAATCAACACCAGGTCGCCGTCGCGCAT  >  CP009273/3047155‑3047446
                                                                                                                                                  |                                                                                                                                                 
agaaagGACGATGGGCGTTCTGAGCGATCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGt                                                                                                                                                  <  1:2931040/148‑1 (MQ=255)
 gaaagGACGATGGGCGTTCTGAGCGATCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGttt                                                                                                                                                 >  2:2438527/1‑148 (MQ=255)
 gaaagGACGATGGGCGTTCTGAGCGATCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGttt                                                                                                                                                 >  2:653270/1‑148 (MQ=255)
  aaagGACGATGGGCGTTCTGAGCGATCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGtttt                                                                                                                                                <  2:2692479/148‑1 (MQ=255)
  aaagGACGATGGGCGTTCTGAGCGATCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGttt                                                                                                                                                >  2:3154979/1‑148 (MQ=255)
    agGACGATGGGCGTTCTGAGCGATCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCg                                                                                                                                              >  2:475877/1‑148 (MQ=255)
    agGACGATGGGCGTTCTGAGCGATCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCg                                                                                                                                              <  1:2222962/148‑1 (MQ=255)
    agGACGATGGGCGTTCTGAGCGATCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACGTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCg                                                                                                                                              <  1:388591/148‑1 (MQ=255)
            gggCGTTCTGAGCGATCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTc                                                                                                                                      >  1:2805912/1‑148 (MQ=255)
             ggCGTTCTGAGCGATCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTcc                                                                                                                                     <  2:1047316/148‑1 (MQ=255)
              gCGTTCTGAGCGATCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCa                                                                                                                                    >  2:1067564/1‑148 (MQ=255)
                  tCTGAGCGATCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGcac                                                                                                                                >  2:901084/1‑148 (MQ=255)
                  tCTGAGCGATCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGcac                                                                                                                                >  2:900258/1‑148 (MQ=255)
                        cGATCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTc                                                                                                                          >  1:1960940/1‑148 (MQ=255)
                          aTCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGt                                                                                                                        >  2:1791599/1‑148 (MQ=255)
                           tCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTa                                                                                                                       >  1:2179738/1‑148 (MQ=255)
                           tCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTa                                                                                                                       >  2:3148633/1‑148 (MQ=255)
                           tCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACACTGTCGTa                                                                                                                       >  2:2704821/1‑148 (MQ=255)
                            cAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAg                                                                                                                      <  1:1689792/148‑1 (MQ=255)
                            cAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAg                                                                                                                      <  1:3449956/148‑1 (MQ=255)
                            cAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAg                                                                                                                      <  2:489623/148‑1 (MQ=255)
                            cAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAg                                                                                                                      <  1:1476965/148‑1 (MQ=255)
                            cAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAg                                                                                                                      <  1:2070832/148‑1 (MQ=255)
                                 catcaACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTc                                                                                                                 >  2:673712/1‑148 (MQ=255)
                                   tcaACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCAc                                                                                                               <  2:3406689/148‑1 (MQ=255)
                                   tcaACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCAc                                                                                                               >  1:305207/1‑148 (MQ=255)
                                    caACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACg                                                                                                              >  1:3165075/1‑148 (MQ=255)
                                      aCATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCt                                                                                                            >  1:2988272/1‑148 (MQ=255)
                                        aTCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTgg                                                                                                          >  2:781011/1‑148 (MQ=255)
                                          cACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGc                                                                                                        >  1:967913/1‑148 (MQ=255)
                                          cACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGc                                                                                                        <  2:1148548/148‑1 (MQ=255)
                                          cACCTGTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGc                                                                                                        <  2:3059999/148‑1 (MQ=255)
                                           aCCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGcc                                                                                                       <  2:2522970/148‑1 (MQ=255)
                                               ttCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTggg                                                                                                   >  1:110790/1‑148 (MQ=255)
                                               ttCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTggg                                                                                                   <  1:764190/148‑1 (MQ=255)
                                                tCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGt                                                                                                  <  2:163970/148‑1 (MQ=255)
                                                  aGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGa                                                                                                <  1:2513129/148‑1 (MQ=255)
                                                    gATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGTGTGAAt                                                                                              <  2:2996941/148‑1 (MQ=255)
                                                    gATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAAt                                                                                              <  1:671812/148‑1 (MQ=255)
                                                      tGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAAttt                                                                                            >  1:2677981/1‑148 (MQ=255)
                                                       gCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTg                                                                                           <  2:1685318/148‑1 (MQ=255)
                                                         cGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGcc                                                                                         <  1:1315764/148‑1 (MQ=255)
                                                         cGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGcc                                                                                         <  1:1315756/148‑1 (MQ=255)
                                                          gAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCg                                                                                        <  2:1571372/148‑1 (MQ=255)
                                                           aGTTTTACCAGGCCGCTAACCATGATGCGCACCACTACACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGt                                                                                       >  2:1778614/1‑148 (MQ=255)
                                                             ttttACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTg                                                                                     <  2:2872191/148‑1 (MQ=255)
                                                              tttACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGa                                                                                    >  1:2094050/1‑148 (MQ=255)
                                                                    aGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGa                                                                              >  2:1468417/1‑148 (MQ=255)
                                                                    aGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGa                                                                              <  2:3019662/148‑1 (MQ=255)
                                                                     ggCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGaa                                                                             >  1:34329/1‑148 (MQ=255)
                                                                     ggCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGaa                                                                             >  1:1834603/1‑148 (MQ=255)
                                                                      gCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAg                                                                            <  1:2498872/148‑1 (MQ=255)
                                                                       ccGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAgg                                                                           <  2:211789/148‑1 (MQ=255)
                                                                       ccGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAgg                                                                           <  2:403226/148‑1 (MQ=255)
                                                                          cTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGc                                                                        >  1:1578588/1‑148 (MQ=255)
                                                                           tAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCg                                                                       <  2:328341/148‑1 (MQ=255)
                                                                               cATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTa                                                                   <  1:2870451/148‑1 (MQ=255)
                                                                               cATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTa                                                                   >  1:2393498/1‑148 (MQ=255)
                                                                                atgatgCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTaa                                                                  <  2:3396416/148‑1 (MQ=255)
                                                                                atgatgCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTaa                                                                  <  2:3249116/148‑1 (MQ=255)
                                                                                 tgatgCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTGTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAat                                                                 >  2:543964/1‑148 (MQ=255)
                                                                                  gatgCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAata                                                                >  1:2046719/1‑148 (MQ=255)
                                                                                   atgCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAatat                                                               <  2:1004617/148‑1 (MQ=255)
                                                                                     gcgcACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCg                                                             >  2:1587482/1‑148 (MQ=255)
                                                                                        caccacTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCa                                                          <  1:863460/148‑1 (MQ=255)
                                                                                         accacTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAg                                                         >  2:1790118/1‑148 (MQ=255)
                                                                                             cTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTa                                                     <  1:3543292/148‑1 (MQ=255)
                                                                                               tCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAAc                                                   <  1:653270/148‑1 (MQ=255)
                                                                                               tCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAAc                                                   <  1:2438527/148‑1 (MQ=255)
                                                                                                cACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAAcc                                                  <  2:186030/148‑1 (MQ=255)
                                                                                                 aCCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCt                                                 <  1:1168150/148‑1 (MQ=255)
                                                                                                 aCCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCt                                                 <  1:790753/148‑1 (MQ=255)
                                                                                                    aGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTg                                              <  1:1477667/148‑1 (MQ=255)
                                                                                                    aGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTg                                              <  1:2436251/148‑1 (MQ=255)
                                                                                                    aGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTg                                              >  1:2654309/1‑148 (MQ=255)
                                                                                                        aCTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTAtt                                          <  2:2425856/148‑1 (MQ=255)
                                                                                                         cTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTc                                         <  2:1709908/148‑1 (MQ=255)
                                                                                                           tCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTcac                                       >  2:2793991/1‑148 (MQ=255)
                                                                                                            ccAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTcaca                                      <  1:2822418/148‑1 (MQ=255)
                                                                                                             cAGAATGGAAGTTCCCGGACGATACAGGCGCTGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACaa                                     <  2:571661/148‑1 (MQ=255)
                                                                                                              aGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAAc                                    >  2:2604446/1‑148 (MQ=255)
                                                                                                                 aTGGAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCcg                                 >  1:3336215/1‑148 (MQ=255)
                                                                                                                   ggAAGTTCCCGGACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCcgcg                               <  2:187689/148‑1 (MQ=255)
                                                                                                                      aGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCCGCGtca                            >  1:3080836/1‑148 (MQ=255)
                                                                                                                        ttCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCCGCGtcaat                          >  2:1608031/1‑148 (MQ=255)
                                                                                                                           ccGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCCGCGtcaatcaa                       <  2:2326856/148‑1 (MQ=255)
                                                                                                                             ggACGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCCGCGTCAATCAaca                     <  2:278921/148‑1 (MQ=255)
                                                                                                                              gACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCCGCGTCAATCAacac                    >  2:3372529/1‑148 (MQ=255)
                                                                                                                              gACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCCGCGTCAATCAacac                    <  1:2791238/148‑1 (MQ=255)
                                                                                                                               aCGATACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCCGCGTCAATCAACAcc                   >  2:2279696/1‑148 (MQ=255)
                                                                                                                               aCGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCCGCGTCAATCAACAcc                   <  2:3510332/148‑1 (MQ=255)
                                                                                                                                  aTACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGACTTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCCGCGTCAATCAACACCAgg                >  2:209611/1‑148 (MQ=255)
                                                                                                                                  aTACAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCCGCGTCAATCAACACCAgg                >  2:1266963/1‑148 (MQ=255)
                                                                                                                                     cAGGCGCAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCCGCGTCAATCAACACCAGGTCg             <  2:1599827/148‑1 (MQ=255)
                                                                                                                                       ggCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCCGCGTCAATCAACACCAGGTCGcc           >  2:307236/1‑148 (MQ=255)
                                                                                                                                        gcgcAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCCGCGTCAATCAACACCAGGTCGCCg          >  2:2159460/1‑148 (MQ=255)
                                                                                                                                        gcgcAGGCTGTTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCCGCGTCAATCAACACCAGGTCGCCg          <  1:1587546/148‑1 (MQ=255)
                                                                                                                                               cTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCCGCGTCAATCAACACCAGGTCGCCGTCGCGCa   <  2:394502/148‑1 (MQ=255)
                                                                                                                                                tGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCCGCGTCAATCAACACCAGGTCGCCGTCGCGCAt  <  1:953909/148‑1 (MQ=255)
                                                                                                                                                tGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCCGCGTCAATCAACACCAGGTCGCCGTCGCGCAt  <  2:110971/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AGAAAGGACGATGGGCGTTCTGAGCGATCAGTTCATCAACATCACCTTTCAGGATGCCGAGTTTTACCAGGCCGCTAACCATGATGCGCACCACTTCACCAGTGACTTCCAGAATGGAAGTTCCCGGACGATACAGGCGCAGGCTGGTTTCGAGAGACTCCAGCACAATGTCGTAGATTTCACGCTGGGCCTGGGTGAATTTGCCGTTGACCGGGAAGGTGCGGGTAATATCGCCAGCGTAACCTTTGTATTCACAACCCGCGTCAATCAACACCAGGTCGCCGTCGCGCAT  >  CP009273/3047155‑3047446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A