Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F368 I0 R1	1472	214.4	7116750	95.9%	6824963	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,074,591	G→T	100%	A135E (GCG→GAG)	tktA ←	transketolase 1, thiamine triphosphate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,074,591	0	G	T	98.6%	731.3 / 0.6	230	A135E (GCG→GAG)	tktA	transketolase 1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand): ref base G (2/2); new base T (136/90); total (138/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 234 total aligned reads displayed.

ATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCA  >  CP009273/3074446‑3074735

  cGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATNATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGctccgc                                                                                                                                              >  1:1800825/1‑148 (MQ=255)

     aGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAg                                                                                                                                           >  1:1787037/1‑148 (MQ=255)

      gAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGc                                                                                                                                          >  2:153113/1‑148 (MQ=255)

      gAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGc                                                                                                                                          >  1:758014/1‑148 (MQ=255)

       aaTGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCg                                                                                                                                         <  2:180400/148‑1 (MQ=255)

       aaTGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCg                                                                                                                                         >  2:3085921/1‑148 (MQ=255)

         tGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGtt                                                                                                                                       >  1:467071/1‑148 (MQ=255)

           cAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGtttt                                                                                                                                     <  2:1000618/148‑1 (MQ=255)

           cAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGtttt                                                                                                                                     <  2:744946/148‑1 (MQ=255)

              tCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTc                                                                                                                                  >  1:381743/1‑148 (MQ=255)

              tCAGTGTACCCAGCTTCAGCGTACCCGCCAGAGAGCAACCTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTc                                                                                                                                  <  2:1850026/148‑1 (MQ=255)

               cAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCt                                                                                                                                 >  2:186396/1‑148 (MQ=255)

               cAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCt                                                                                                                                 >  1:324151/1‑148 (MQ=255)

                aGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTg                                                                                                                                >  2:2689721/1‑148 (MQ=255)

                  tttACCCAGCTTTAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCa                                                                                                                              >  1:278568/1‑148 (MQ=255)

                  tttACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCa                                                                                                                              <  2:1971340/148‑1 (MQ=255)

                   ttACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCaa                                                                                                                             <  1:688651/148‑1 (MQ=255)

                        cAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGcc                                                                                                                        <  2:515614/148‑1 (MQ=255)

                        cAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGcc                                                                                                                        <  2:2756140/148‑1 (MQ=255)

                            ttCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATAc                                                                                                                    >  1:3246811/1‑148 (MQ=255)

                            ttCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATAc                                                                                                                    >  1:2861278/1‑148 (MQ=255)

                                   tACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGc                                                                                                             >  2:3342324/1‑148 (MQ=255)

                                     cccGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGt                                                                                                           <  1:1075050/148‑1 (MQ=255)

                                         ccAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGc                                                                                                       >  1:92545/1‑148 (MQ=255)

                                         ccAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGc                                                                                                       <  1:1996978/148‑1 (MQ=255)

                                           agagagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCaa                                                                                                     >  2:633953/1‑148 (MQ=255)

                                           agagagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCaa                                                                                                     <  2:3470592/148‑1 (MQ=255)

                                            gagagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAAt                                                                                                    <  1:1317053/148‑1 (MQ=255)

                                            gagagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAAt                                                                                                    >  1:293873/1‑148 (MQ=255)

                                             agagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATa                                                                                                   <  2:3336754/148‑1 (MQ=255)

                                               agCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATAcc                                                                                                 >  1:3226639/1‑148 (MQ=255)

                                               agCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATAcc                                                                                                 >  1:2396489/1‑148 (MQ=255)

                                                  aaaCTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTg                                                                                              <  1:2586863/148‑1 (MQ=255)

                                                   accTTCGTGGGAGATGCCGTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTTGTCGACTATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGa                                                                                             <  1:3453420/146‑1 (MQ=255)

                                                        cGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAg                                                                                        >  2:3514256/1‑148 (MQ=255)

                                                         gTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGc                                                                                       <  2:3547520/148‑1 (MQ=255)

                                                           gggAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCgg                                                                                     >  1:1848098/1‑148 (MQ=255)

                                                           gggAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCgg                                                                                     <  2:52483/148‑1 (MQ=255)

                                                            ggAGATGCCTTCCATCATGCAGCCGTAGCCCATGAAGGCGTAGGTGTAGTGGTCGCCAATGTCGTGGCCCGGGCGGTTAAACTCCTCCGCCAGCGTTTTTTCTGCACTCGCCAGACCCACTGCGTTGGCAATCCCCGGACCCAGCggc                                                                                    >  2:2187651/1‑147 (MQ=255)

                                                              agaTGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGAcc                                                                                  >  1:3202343/1‑148 (MQ=255)

                                                              agaTGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGAcc                                                                                  >  1:2858635/1‑148 (MQ=255)

                                                              agaTGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGAcc                                                                                  >  1:1591131/1‑148 (MQ=255)

                                                                aTGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCgg                                                                                >  1:205376/1‑148 (MQ=255)

                                                                aTGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCgg                                                                                >  1:814947/1‑148 (MQ=255)

                                                                     ttCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCggtggtg                                                                           <  1:2685859/148‑1 (MQ=255)

                                                                         atcatGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGttt                                                                       <  2:91261/148‑1 (MQ=255)

                                                                          tcatGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTc                                                                      >  1:3027140/1‑148 (MQ=255)

                                                                           catGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTcc                                                                     >  1:852830/1‑148 (MQ=255)

                                                                           catGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTcc                                                                     <  2:1032714/148‑1 (MQ=255)

                                                                            atGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCca                                                                    <  2:1104697/148‑1 (MQ=255)

                                                                              gCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCcaca                                                                  >  1:2488787/1‑148 (MQ=255)

                                                                              gCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCcaca                                                                  >  1:2488239/1‑148 (MQ=255)

                                                                                aGCCGTCGCCCATGAAGGCGTAGGTGGAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACAcc                                                                <  1:1518936/148‑1 (MQ=255)

                                                                                      cGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGgt                                                          >  2:2075096/1‑148 (MQ=255)

                                                                                      cGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGgt                                                          <  2:2230927/148‑1 (MQ=255)

                                                                                      cGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGgt                                                          <  1:1281682/148‑1 (MQ=255)

                                                                                      cGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGgt                                                          >  2:1480242/1‑148 (MQ=255)

                                                                                         ccATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTa                                                       >  1:2643749/1‑148 (MQ=255)

                                                                                            tGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAAcc                                                    <  2:1898824/148‑1 (MQ=255)

                                                                                            tGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAAcc                                                    <  2:982168/148‑1 (MQ=255)

                                                                                             gAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAAccc                                                   >  2:33908/1‑148 (MQ=255)

                                                                                              aaGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCa                                                  >  2:722889/1‑148 (MQ=255)

                                                                                                        tgtAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTg                                        <  1:1687317/148‑1 (MQ=255)

                                                                                                        tgtAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTg                                        <  1:633275/148‑1 (MQ=255)

                                                                                                          tAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGAc                                      >  1:1263772/1‑148 (MQ=255)

                                                                                                          tAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGAc                                      >  2:1943170/1‑148 (MQ=255)

                                                                                                          tAGTGGTCGACAATGTCGTGGCCCGGACGCTAAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCAAACCGACTGCGTTGGCAATAACCTTACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTACCGGGTTAc                                      >  1:3117193/1‑148 (MQ=255)

                                                                                                            gTGGACGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGAccc                                    <  1:3556270/148‑1 (MQ=255)

                                                                                                             tGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCg                                   >  2:1895397/1‑148 (MQ=255)

                                                                                                             tGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCg                                   >  2:2861056/1‑148 (MQ=255)

                                                                                                                 cGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGt                               <  2:2673171/148‑1 (MQ=255)

                                                                                                                    cAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGtttt                            >  2:151040/1‑148 (MQ=255)

                                                                                                                    cAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGtttt                            >  2:1347278/1‑148 (MQ=255)

                                                                                                                    cAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGtttt                            >  2:2242747/1‑148 (MQ=255)

                                                                                                                       tGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCCTACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTaga                         >  1:1525675/1‑148 (MQ=255)

                                                                                                                       tGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTaga                         >  1:1707533/1‑148 (MQ=255)

                                                                                                                             ggCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAg                   <  1:510896/148‑1 (MQ=255)

                                                                                                                             ggCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAg                   <  1:2994247/148‑1 (MQ=255)

                                                                                                                              gCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGc                  >  1:1442635/1‑148 (MQ=255)

                                                                                                                                 cGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGa               <  1:1948588/148‑1 (MQ=255)

                                                                                                                                  ggACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGAc              >  1:2177240/1‑148 (MQ=255)

                                                                                                                                   gACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACg             >  2:2374963/1‑148 (MQ=255)

                                                                                                                                   gACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTGTTAGAGTGCAGCTGACg             >  2:2374997/1‑148 (MQ=255)

                                                                                                                                    aCGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACgg            >  1:1434/1‑148 (MQ=255)

                                                                                                                                       gTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGTAg         >  1:2285510/1‑148 (MQ=255)

                                                                                                                                       gTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAg         <  2:3417909/148‑1 (MQ=255)

                                                                                                                                       gTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAg         >  1:3378158/1‑148 (MQ=255)

                                                                                                                                       gTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAg         >  2:2352326/1‑148 (MQ=255)

                                                                                                                                        ttAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGCCCCGGAGTTTTAGAGTGCAGCTGACGGAAGt        <  2:314923/148‑1 (MQ=255)

                                                                                                                                        ttAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGt                               >  2:3248824/1‑125 (MQ=255)

                                                                                                                                        ttAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGt                               <  1:3248824/125‑1 (MQ=255)

                                                                                                                                        ttAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGt        >  2:2609677/1‑148 (MQ=255)

                                                                                                                                          aaaCTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGttt      <  2:323961/148‑1 (MQ=255)

                                                                                                                                          aaaCTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGttt      >  1:1729653/1‑148 (MQ=255)

                                                                                                                                           aaCTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGtttt     >  2:3000926/1‑148 (MQ=255)

                                                                                                                                           aaCTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGtttt     >  2:3526076/1‑148 (MQ=255)

                                                                                                                                            aCTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATTCCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGttttt    <  2:1787020/148‑1 (MQ=255)

                                                                                                                                            aCTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGttttt    <  2:278568/148‑1 (MQ=255)

                                                                                                                                             cTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTc   <  1:326798/148‑1 (MQ=255)

                                                                                                                                              tGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCTGCGGACCGGTGGTGGTTTCCACACCAGAGGTGTAACCCACTTCCTGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCa  <  2:1267771/148‑1 (MQ=255)

ATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCA  >  CP009273/3074446‑3074735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTC  >  CP009273/3074443‑3074739

GTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTC                                                                                                                                                     >  SRR19973067.2766975/1‑150 (MQ=60)

GTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTC                                                                                                                                                     >  SRR19973067.2766958/1‑150 (MQ=60)

   ATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATNATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGC                                                                                                                                                  >  SRR19973067.1812924/1‑150 (MQ=60)

   ATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATNATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGC                                                                                                                                                  >  SRR19973067.1812822/1‑150 (MQ=60)

   ATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGC                                                                                                                                                  >  SRR19973067.2006867/1‑150 (MQ=60)

      GTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAG                                                                                                                                               >  SRR19973067.1799092/1‑150 (MQ=60)

      GTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAG                                                                                                                                               >  SRR19973067.1799075/1‑150 (MQ=60)

       TAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGC                                                                                                                                              >  SRR19973067.763281/1‑150 (MQ=60)

        AGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCG                                                                                                                                             >  SRR19973067.3162116/1‑150 (MQ=60)

        AGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCG                                                                                                                                             >  SRR19973067.1455073/1‑150 (MQ=60)

          AATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTT                                                                                                                                           <  SRR19973067.612854/150‑1 (MQ=60)

          AATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTT                                                                                                                                           <  SRR19973067.3263829/150‑1 (MQ=60)

          AATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTT                                                                                                                                           >  SRR19973067.470303/1‑150 (MQ=60)

           ATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTT                                                                                                                                          >  SRR19973067.650011/1‑150 (MQ=60)

               AATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTC                                                                                                                                      >  SRR19973067.384403/1‑150 (MQ=60)

               AATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTC                                                                                                                                      <  SRR19973067.3523134/150‑1 (MQ=60)

                ATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCT                                                                                                                                     <  SRR19973067.2972569/150‑1 (MQ=60)

                ATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCT                                                                                                                                     >  SRR19973067.326166/1‑150 (MQ=60)

                ATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCT                                                                                                                                     >  SRR19973067.326358/1‑150 (MQ=60)

                   AGTTTACCCAGCTTTAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCA                                                                                                                                  >  SRR19973067.280408/1‑150 (MQ=60)

                      TTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATC                                                                                                                               <  SRR19973067.693394/150‑1 (MQ=60)

                      TTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATC                                                                                                                               >  SRR19973067.2859001/1‑150 (MQ=60)

                          CCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCA                                                                                                                           <  SRR19973067.751970/150‑1 (MQ=60)

                            AGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATA                                                                                                                         >  SRR19973067.2047581/1‑150 (MQ=60)

                             GCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATAC                                                                                                                        >  SRR19973067.3267120/1‑150 (MQ=60)

                             GCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATAC                                                                                                                        >  SRR19973067.3267142/1‑150 (MQ=60)

                             GCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATAC                                                                                                                        >  SRR19973067.2879590/1‑150 (MQ=60)

                             GCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATAC                                                                                                                        >  SRR19973067.3267147/1‑150 (MQ=60)

                             GCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATAC                                                                                                                        >  SRR19973067.2879616/1‑150 (MQ=60)

                               TTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCG                                                                                                                      >  SRR19973067.3283689/1‑150 (MQ=60)

                                  AGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACT                                                                                                                   <  SRR19973067.2481612/150‑1 (MQ=60)

                                       ACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTT                                                                                                              <  SRR19973067.1514571/150‑1 (MQ=60)

                                       ACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTACTGAAATCGCCATACCTACTCCGTT                                                                                                              >  SRR19973067.1276381/1‑150 (MQ=60)

                                        CCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTG                                                                                                             <  SRR19973067.1082421/150‑1 (MQ=60)

                                          CGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGC                                                                                                           >  SRR19973067.93521/1‑150 (MQ=60)

                                          CGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGC                                                                                                           >  SRR19973067.93440/1‑150 (MQ=60)

                                            CCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAA                                                                                                         <  SRR19973067.2010459/150‑1 (MQ=60)

                                             CAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAAT                                                                                                        >  SRR19973067.295757/1‑150 (MQ=60)

                                              AGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATA                                                                                                       >  SRR19973067.1308256/1‑150 (MQ=60)

                                               GAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATAC                                                                                                      <  SRR19973067.383557/150‑1 (MQ=60)

                                               GAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATAC                                                                                                      >  SRR19973067.2150262/1‑150 (MQ=60)

                                               GAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATAC                                                                                                      <  SRR19973067.1326045/150‑1 (MQ=60)

                                                AGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACC                                                                                                     >  SRR19973067.2412102/1‑150 (MQ=60)

                                                AGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACC                                                                                                     >  SRR19973067.3246904/1‑150 (MQ=60)

                                                     AAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAC                                                                                                <  SRR19973067.809808/150‑1 (MQ=60)

                                                     AAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAC                                                                                                <  SRR19973067.2603706/150‑1 (MQ=60)

                                                     AAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAC                                                                                                >  SRR19973067.174667/1‑150 (MQ=60)

                                                     AAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGATTTTTCTGCAATCGCCAAACCGACTGCGTTGGCAATACCCTGAC                                                                                                >  SRR19973067.174336/1‑150 (MQ=60)

                                                      ACCTTCGTGGGAGATGCCGTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTTGTCGACTATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACC                                                                                               <  SRR19973067.3475391/150‑1 (MQ=60)

                                                        CTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCA                                                                                             <  SRR19973067.2818523/150‑1 (MQ=60)

                                                            GTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGG                                                                                         >  SRR19973067.1860694/1‑150 (MQ=60)

                                                               GGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACC                                                                                      >  SRR19973067.3222537/1‑150 (MQ=60)

                                                               GGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACC                                                                                      >  SRR19973067.2876962/1‑150 (MQ=60)

                                                               GGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACC                                                                                      >  SRR19973067.1602037/1‑150 (MQ=60)

                                                                 AGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGG                                                                                    >  SRR19973067.820534/1‑150 (MQ=60)

                                                                 AGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGG                                                                                    >  SRR19973067.206926/1‑150 (MQ=60)

                                                                      CCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTG                                                                               >  SRR19973067.2563493/1‑150 (MQ=60)

                                                                      CCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTG                                                                               >  SRR19973067.2991681/1‑150 (MQ=60)

                                                                       CTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGG                                                                              >  SRR19973067.2887213/1‑150 (MQ=60)

                                                                       CTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGG                                                                              >  SRR19973067.32601/1‑150 (MQ=60)

                                                                       CTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGG                                                                              >  SRR19973067.2690323/1‑150 (MQ=60)

                                                                        TTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGT                                                                             <  SRR19973067.2703090/150‑1 (MQ=60)

                                                                           CATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTC                                                                          >  SRR19973067.3046414/1‑150 (MQ=60)

                                                                            ATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCC                                                                         >  SRR19973067.858587/1‑150 (MQ=60)

                                                                            ATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCC                                                                         >  SRR19973067.858588/1‑150 (MQ=60)

                                                                               ATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACA                                                                      >  SRR19973067.2504595/1‑150 (MQ=60)

                                                                               ATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACA                                                                      >  SRR19973067.2505146/1‑150 (MQ=60)

                                                                               ATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACA                                                                      >  SRR19973067.2504600/1‑150 (MQ=60)

                                                                                   AGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAG                                                                  <  SRR19973067.1529137/150‑1 (MQ=60)

                                                                                   AGCCGTCGCCCATGAAGGCGTAGGTGGAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAG                                                                  <  SRR19973067.1529090/150‑1 (MQ=60)

                                                                                      CGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGG                                                               >  SRR19973067.428887/1‑150 (MQ=60)

                                                                                      CGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGG                                                               >  SRR19973067.95528/1‑150 (MQ=60)

                                                                                      CGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGG                                                               >  SRR19973067.36729/1‑150 (MQ=60)

                                                                                         CGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGT                                                            <  SRR19973067.1290460/150‑1 (MQ=60)

                                                                                         CGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGT                                                            <  SRR19973067.1290413/150‑1 (MQ=60)

                                                                                          GCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTA                                                           >  SRR19973067.2660824/1‑150 (MQ=60)

                                                                                                 AAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACT                                                    >  SRR19973067.3439307/1‑150 (MQ=60)

                                                                                                    GCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCC                                                 >  SRR19973067.22428/1‑150 (MQ=60)

                                                                                                     CGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCG                                                <  SRR19973067.2436981/150‑1 (MQ=60)

                                                                                                       TAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGG                                              >  SRR19973067.900856/1‑150 (MQ=60)

                                                                                                         GGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTG                                            <  SRR19973067.481161/150‑1 (MQ=60)

                                                                                                         GGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTG                                            >  SRR19973067.784648/1‑150 (MQ=60)

                                                                                                           TGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGAC                                          <  SRR19973067.637517/150‑1 (MQ=60)

                                                                                                           TGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGAC                                          <  SRR19973067.1698941/150‑1 (MQ=60)

                                                                                                           TGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGAC                                          >  SRR19973067.1272348/1‑150 (MQ=60)

                                                                                                           TGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGAC                                          >  SRR19973067.3054297/1‑150 (MQ=60)

                                                                                                           TGTAGTGGTCGACAATGTCGTGGCCCGGACGCTAAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCAAACCGACTGCGTTGGCAATAACCTTACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTACCGGGTTAC                                          >  SRR19973067.3137010/1‑150 (MQ=60)

                                                                                                              AGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCG                                       <  SRR19973067.3391285/150‑1 (MQ=60)

                                                                                                               GTGGACGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGG                                      <  SRR19973067.3578680/150‑1 (MQ=60)

                                                                                                                 GGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAG                                    <  SRR19973067.195713/150‑1 (MQ=60)

                                                                                                                 GGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAG                                    <  SRR19973067.195315/150‑1 (MQ=60)

                                                                                                                    agacgtgtgctcttccgatctGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTT                                 <  SRR19973067.3269161/129‑1 (MQ=60)

                                                                                                                     GACCAGGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTGTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTT                                <  SRR19973067.1844683/150‑1 (MQ=60)

                                                                                                                     GACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTT                                <  SRR19973067.602087/150‑1 (MQ=60)

                                                                                                                        AATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCCTACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGA                             >  SRR19973067.1535929/1‑150 (MQ=60)

                                                                                                                        AATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGA                             >  SRR19973067.1718635/1‑150 (MQ=60)

                                                                                                                        AATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGA                             >  SRR19973067.1719243/1‑150 (MQ=60)

                                                                                                                         ATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAG                            <  SRR19973067.2724893/150‑1 (MQ=60)

                                                                                                                             CGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCA                        <  SRR19973067.2658689/150‑1 (MQ=60)

                                                                                                                               TGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGC                      >  SRR19973067.1452333/1‑150 (MQ=60)

                                                                                                                               TGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGC                      >  SRR19973067.1452278/1‑150 (MQ=60)

                                                                                                                                GGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCT                     <  SRR19973067.3013227/150‑1 (MQ=60)

                                                                                                                                GGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCT                     <  SRR19973067.514378/150‑1 (MQ=60)

                                                                                                                                   CCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGAC                  <  SRR19973067.26414/150‑1 (MQ=60)

                                                                                                                                   CCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGAC                  >  SRR19973067.2191802/1‑150 (MQ=60)

                                                                                                                                   CCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGAC                  <  SRR19973067.3173433/150‑1 (MQ=60)

                                                                                                                                    CGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACG                 <  SRR19973067.1961863/150‑1 (MQ=60)

                                                                                                                                     GGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGG                >  SRR19973067.1651019/1‑150 (MQ=60)

                                                                                                                                     GGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGG                >  SRR19973067.1459/1‑150 (MQ=60)

                                                                                                                                     GGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGG                >  SRR19973067.1651009/1‑150 (MQ=60)

                                                                                                                                        CGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGTAG             >  SRR19973067.2300618/1‑150 (MQ=60)

                                                                                                                                        CGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAG             >  SRR19973067.3399631/1‑150 (MQ=60)

                                                                                                                                        CGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCCACTGCGTTGGCAATACCCTGACCCAGCGGACCGGGGGTGGTTTCCACACCAGCGGTGTAACCCACTTCAGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAG             >  SRR19973067.3399196/1‑150 (MQ=60)

                                                                                                                                          GTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTT           <  SRR19973067.2176341/150‑1 (MQ=60)

                                                                                                                                          GTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTT           <  SRR19973067.1402677/150‑1 (MQ=60)

                                                                                                                                           TTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTT          >  SRR19973067.1741496/1‑150 (MQ=60)

                                                                                                                                           TTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTT          >  SRR19973067.1538447/1‑150 (MQ=60)

                                                                                                                                           TTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTT          >  SRR19973067.1266232/1‑150 (MQ=60)

                                                                                                                                             AAACTGCTCCGCCAGCGTTTTTTCTGCAATAGCCATAACGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTT        >  SRR19973067.3021346/1‑150 (MQ=60)

                                                                                                                                              AACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTC       >  SRR19973067.23424/1‑150 (MQ=60)

                                                                                                                                                CTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAG     <  SRR19973067.329029/150‑1 (MQ=60)

                                                                                                                                                   CTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTC  <  SRR19973067.187850/150‑1 (MQ=60)

                                                                                                                                                   CTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTC  <  SRR19973067.187801/150‑1 (MQ=60)

                                                                                                                                                   CTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTC  <  SRR19973067.2937248/150‑1 (MQ=60)

                                                                                                                                                   CTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTC  <  SRR19973067.187856/150‑1 (MQ=60)

                                                                                                                                                   CTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTC  <  SRR19973067.2937181/150‑1 (MQ=60)

                                                                                                                                                   CTCCGCCAGCGTTTTTTCTGCAATCGCCATAACGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACATCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTC  >  SRR19973067.931973/1‑150 (MQ=60)

GTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTC  >  CP009273/3074443‑3074739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`