Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,074,591 G→T 100% A135E (GCG→GAG)  tktA ← transketolase 1, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,074,5910GT99.1% 700.4 / ‑2.0 217A135E (GCG→GAG) tktAtransketolase 1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (110/105);  total (112/105)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 222 total aligned reads displayed.

ATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTC  >  CP009273/3074446‑3074734
                                                                                                                                                 |                                                                                                                                               
aTCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGctcc                                                                                                                                               >  1:2591416/1‑148 (MQ=255)
aTCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGctcc                                                                                                                                               >  2:565842/1‑148 (MQ=255)
    tAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCa                                                                                                                                           >  2:2324271/1‑148 (MQ=255)
      gAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGc                                                                                                                                         <  1:266719/148‑1 (MQ=255)
      gAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGc                                                                                                                                         <  1:2066567/148‑1 (MQ=255)
        aTGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGt                                                                                                                                       >  1:2789175/1‑148 (MQ=255)
         tGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGtt                                                                                                                                      <  2:1928908/148‑1 (MQ=255)
          gaaaTCAGTTTACACAGCATCCGCGTACCCGCCAGAGAGCAAACGTAGTGGGAGATGCCATCCATCTTGCAGCCGGCGCCCATGAAGGCGTAGGGGGAGTGGTCGACACTGTCGAGGCCCGGACGTTTAAACTGCTCCGCCAGCGtat                                                                                                                                     >  2:1279197/3‑146 (MQ=255)
          gCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTCGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGttt                                                                                                                                     >  2:3085091/1‑148 (MQ=255)
            aaTCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGttttt                                                                                                                                   <  2:1009289/148‑1 (MQ=255)
            aaTCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGttttt                                                                                                                                   <  1:2840744/148‑1 (MQ=255)
                  tttACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCa                                                                                                                             >  1:367557/1‑148 (MQ=255)
                  tttACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTAGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCa                                                                                                                             >  2:968889/1‑148 (MQ=255)
                   ttACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCaa                                                                                                                            >  1:1646505/1‑148 (MQ=255)
                   ttACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCaa                                                                                                                            <  2:782110/148‑1 (MQ=255)
                    tACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAAt                                                                                                                           <  1:3112706/148‑1 (MQ=255)
                    gaCCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAAt                                                                                                                           <  2:2790766/147‑1 (MQ=255)
                       ccAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGc                                                                                                                        <  2:2188126/148‑1 (MQ=255)
                         aGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCa                                                                                                                      >  1:2318364/1‑148 (MQ=255)
                          gCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCAt                                                                                                                     <  2:1056431/148‑1 (MQ=255)
                          gCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCAt                                                                                                                     >  2:2392634/1‑148 (MQ=255)
                          gCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCAt                                                                                                                     >  2:602206/1‑148 (MQ=255)
                             tCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATAcc                                                                                                                  >  1:353922/1‑148 (MQ=255)
                                gCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGAc                                                                                                               <  1:80254/148‑1 (MQ=255)
                                    aCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCg                                                                                                           >  1:1444201/1‑148 (MQ=255)
                                    aCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCg                                                                                                           >  1:1555163/1‑148 (MQ=255)
                                    aCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCg                                                                                                           <  2:2607057/148‑1 (MQ=255)
                                    aCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCg                                                                                                           >  1:1208910/1‑148 (MQ=255)
                                     cccGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGt                                                                                                          >  1:2557531/1‑148 (MQ=255)
                                       cGCCACAGAGCAAACTACGTGGTAGATCAATTCCATCATGAAGCCGTCTCACAACAACGAGTCGTTGTCGTCTTCGACAATGTCGTGGCCCGGACGGATAAACTGCTCCGCCAGCGTTTTTTCTGAAATCGCCATAACGACTGCGTTg                                                                                                        >  2:1887875/1‑148 (MQ=255)
                                        gCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTgg                                                                                                       <  2:2827581/148‑1 (MQ=255)
                                         ccAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGc                                                                                                      >  2:2003265/1‑148 (MQ=255)
                                          cAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCa                                                                                                     >  1:45511/1‑148 (MQ=255)
                                          cAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCa                                                                                                     <  2:67079/148‑1 (MQ=255)
                                           agagagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATAGCCATACCGACTGCGTTGGCaa                                                                                                    <  1:1882175/148‑1 (MQ=255)
                                            gagagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAAt                                                                                                   >  1:2806624/1‑148 (MQ=255)
                                            gagagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAAt                                                                                                   >  1:2533297/1‑148 (MQ=255)
                                             agagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATa                                                                                                  >  1:2960922/1‑148 (MQ=255)
                                             agagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATa                                                                                                  <  1:3348855/148‑1 (MQ=255)
                                                gCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATAccc                                                                                               <  2:2172481/148‑1 (MQ=255)
                                                  aaaCTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTg                                                                                             >  1:408933/1‑148 (MQ=255)
                                                  aaaCTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTg                                                                                             <  2:3189762/148‑1 (MQ=255)
                                                    aCTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAc                                                                                           <  2:1375139/148‑1 (MQ=255)
                                                    aCTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAc                                                                                           >  2:1516957/1‑148 (MQ=255)
                                                    aCTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAc                                                                                           >  2:2348907/1‑148 (MQ=255)
                                                     cTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAcc                                                                                          <  1:2376585/148‑1 (MQ=255)
                                                     cTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAcc                                                                                          <  2:349670/148‑1 (MQ=255)
                                                     cTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAcc                                                                                          <  2:2950673/148‑1 (MQ=255)
                                                      ttCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAccc                                                                                         <  2:895430/148‑1 (MQ=255)
                                                      ttCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAccc                                                                                         >  1:907557/1‑148 (MQ=255)
                                                                aTGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCgg                                                                               <  1:778536/148‑1 (MQ=255)
                                                                aTGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGGTAAACTGCTCCGCCCGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATAGCCTGACCCAGCGGACCgg                                                                               >  2:2094745/1‑148 (MQ=255)
                                                                  gCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCggtg                                                                             >  1:1667685/1‑148 (MQ=255)
                                                                  gCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCggtg                                                                             >  1:1146073/1‑148 (MQ=255)
                                                                    cTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCggtggt                                                                           >  1:3408390/1‑148 (MQ=255)
                                                                     ttCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCggtggtg                                                                          <  1:1043114/148‑1 (MQ=255)
                                                                     ttCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCggtggtg                                                                          >  1:1340405/1‑148 (MQ=255)
                                                                      tCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCggtggtgg                                                                         <  1:600215/148‑1 (MQ=255)
                                                                          tcatGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTc                                                                     <  2:356315/148‑1 (MQ=255)
                                                                                  ccGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAg                                                             >  2:9197/1‑148 (MQ=255)
                                                                                     tCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCgg                                                          >  1:637587/1‑148 (MQ=255)
                                                                                     tCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCgg                                                          >  1:1772257/1‑148 (MQ=255)
                                                                                          cATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTaa                                                     <  1:3130657/148‑1 (MQ=255)
                                                                                              aaGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCa                                                 <  1:2331631/148‑1 (MQ=255)
                                                                                              aaGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCa                                                 <  1:711530/148‑1 (MQ=255)
                                                                                                ggCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACt                                               >  2:2218935/1‑148 (MQ=255)
                                                                                                  cGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTc                                             >  1:208031/1‑148 (MQ=255)
                                                                                                  cGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTc                                             <  1:854667/148‑1 (MQ=255)
                                                                                                      ggTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCggg                                         >  1:1841042/1‑148 (MQ=255)
                                                                                                      ggTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCggg                                         >  1:2310017/1‑148 (MQ=255)
                                                                                                      ggTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCggg                                         >  1:873974/1‑148 (MQ=255)
                                                                                                       gtgtAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGt                                        >  1:94479/1‑148 (MQ=255)
                                                                                                        tgtAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTg                                       >  2:2666576/1‑148 (MQ=255)
                                                                                                         gtAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGa                                      <  2:740131/148‑1 (MQ=255)
                                                                                                          tAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGAc                                     <  1:2187352/148‑1 (MQ=255)
                                                                                                           aGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGAcc                                    <  2:1233702/148‑1 (MQ=255)
                                                                                                             tGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCg                                  >  2:783133/1‑148 (MQ=255)
                                                                                                             tGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCg                                  >  1:2040897/1‑148 (MQ=255)
                                                                                                             tGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCg                                  >  1:3305935/1‑148 (MQ=255)
                                                                                                              ggTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCgg                                 <  2:1385862/148‑1 (MQ=255)
                                                                                                                 cGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGAATGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGt                              >  2:90774/1‑148 (MQ=255)
                                                                                                                   aCAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGttt                            <  1:2290138/148‑1 (MQ=255)
                                                                                                                    cAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACTCCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGtttt                           >  2:1862028/1‑148 (MQ=255)
                                                                                                                    cAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGgttt                           >  2:3427733/1‑148 (MQ=255)
                                                                                                                      aTGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTGTTag                         >  1:436563/1‑148 (MQ=255)
                                                                                                                        gTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTGTagag                       >  2:1447181/1‑148 (MQ=255)
                                                                                                                          cGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTg                     <  2:3377690/148‑1 (MQ=255)
                                                                                                                            tGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCa                   >  1:3108009/1‑148 (MQ=255)
                                                                                                                              gCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGc                 <  1:3054467/148‑1 (MQ=255)
                                                                                                                                ccGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTg               <  1:1463908/148‑1 (MQ=255)
                                                                                                                                ccGGACGGTTAAAATGCTCCGCCAGCGTTTTTTCTTCAATCGCCATACCGACTGCGTTTTCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTg               <  1:1464564/148‑1 (MQ=255)
                                                                                                                                   gACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACg            >  1:949915/1‑148 (MQ=255)
                                                                                                                                    aCGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACgg           <  2:2591416/148‑1 (MQ=255)
                                                                                                                                    aCGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACAAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACgg           >  2:538558/1‑148 (MQ=255)
                                                                                                                                    aCGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACAAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACgg           >  2:1698330/1‑148 (MQ=255)
                                                                                                                                        ttAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGt       <  2:1121789/148‑1 (MQ=255)
                                                                                                                                          aaaCTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGttt     <  2:2773471/148‑1 (MQ=255)
                                                                                                                                           aaCTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGtttt    >  1:1556966/1‑148 (MQ=255)
                                                                                                                                           aaCTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGGGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGtttt    >  1:2767519/1‑148 (MQ=255)
                                                                                                                                             cTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTc  >  1:1013323/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
ATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTC  >  CP009273/3074446‑3074734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCT  >  CP009273/3074444‑3074740
                                                                                                                                                   |                                                                                                                                                     
TCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCC                                                                                                                                                     >  SRR19973066.1829486/1‑150 (MQ=60)
TCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCC                                                                                                                                                     >  SRR19973066.2608974/1‑150 (MQ=60)
 CATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCG                                                                                                                                                    <  SRR19973066.1261/150‑1 (MQ=60)
    CGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCA                                                                                                                                                 <  SRR19973066.2286843/150‑1 (MQ=60)
        GAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGT                                                                                                                                             >  SRR19973066.2807946/1‑150 (MQ=60)
        GAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGT                                                                                                                                             <  SRR19973066.2080877/150‑1 (MQ=60)
        GAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGT                                                                                                                                             <  SRR19973066.268709/150‑1 (MQ=60)
          ATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTT                                                                                                                                           <  SRR19973066.1670204/150‑1 (MQ=60)
           TGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTT                                                                                                                                          <  SRR19973066.623214/150‑1 (MQ=60)
            GCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTT                                                                                                                                         <  SRR19973066.1592315/150‑1 (MQ=60)
              AATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTC                                                                                                                                       <  SRR19973066.2859781/150‑1 (MQ=60)
              AATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTC                                                                                                                                       <  SRR19973066.2859837/150‑1 (MQ=60)
                  AGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCA                                                                                                                                   >  SRR19973066.370442/1‑150 (MQ=60)
                   GTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAA                                                                                                                                  >  SRR19973066.1658496/1‑150 (MQ=60)
                     TTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATC                                                                                                                                <  SRR19973066.2094506/150‑1 (MQ=60)
                      TACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCG                                                                                                                               <  SRR19973066.3133494/150‑1 (MQ=60)
                        CCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCC                                                                                                                             <  SRR19973066.1089425/150‑1 (MQ=60)
                         CCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCA                                                                                                                            >  SRR19973066.2220448/1‑150 (MQ=60)
                         CCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCA                                                                                                                            >  SRR19973066.2334215/1‑150 (MQ=60)
                             CTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACC                                                                                                                        >  SRR19973066.356675/1‑150 (MQ=60)
                             CTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACC                                                                                                                        >  SRR19973066.3082602/1‑150 (MQ=60)
                                 AGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACT                                                                                                                    >  SRR19973066.2970377/1‑150 (MQ=60)
                                  GCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTG                                                                                                                   <  SRR19973066.369366/150‑1 (MQ=60)
                                  GCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTG                                                                                                                   <  SRR19973066.81171/150‑1 (MQ=60)
                                    GTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCG                                                                                                                 >  SRR19973066.176187/1‑150 (MQ=60)
                                    GTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCG                                                                                                                 >  SRR19973066.1454476/1‑150 (MQ=60)
                                    GTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCG                                                                                                                 >  SRR19973066.1217359/1‑150 (MQ=60)
                                    GTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCG                                                                                                                 >  SRR19973066.1454562/1‑150 (MQ=60)
                                    GTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCG                                                                                                                 >  SRR19973066.3003561/1‑150 (MQ=60)
                                    GTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCG                                                                                                                 >  SRR19973066.1566342/1‑150 (MQ=60)
                                     TACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGT                                                                                                                >  SRR19973066.2574893/1‑150 (MQ=60)
                                      ACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTT                                                                                                               >  SRR19973066.450693/1‑150 (MQ=60)
                                         CGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGC                                                                                                            >  SRR19973066.852847/1‑150 (MQ=60)
                                         CGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGC                                                                                                            >  SRR19973066.851872/1‑150 (MQ=60)
                                          GCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCA                                                                                                           >  SRR19973066.46109/1‑150 (MQ=60)
                                            CAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAAT                                                                                                         >  SRR19973066.2550519/1‑150 (MQ=60)
                                            CAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAAT                                                                                                         >  SRR19973066.2825508/1‑150 (MQ=60)
                                             AGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATA                                                                                                        >  SRR19973066.2980530/1‑150 (MQ=60)
                                             AGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATA                                                                                                        >  SRR19973066.2980492/1‑150 (MQ=60)
                                             AGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATAGCCATACCGACTGCGTTGGCAATA                                                                                                        <  SRR19973066.1895608/150‑1 (MQ=60)
                                               AGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACC                                                                                                      <  SRR19973066.3371160/150‑1 (MQ=60)
                                                  GCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTG                                                                                                   >  SRR19973066.412145/1‑150 (MQ=60)
                                                  GCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTG                                                                                                   >  SRR19973066.412194/1‑150 (MQ=60)
                                                      ACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCC                                                                                               >  SRR19973066.913971/1‑150 (MQ=60)
                                                       CTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCA                                                                                              <  SRR19973066.401015/150‑1 (MQ=60)
                                                       CTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCA                                                                                              <  SRR19973066.1500463/150‑1 (MQ=60)
                                                       CTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCA                                                                                              <  SRR19973066.2392582/150‑1 (MQ=60)
                                                       CTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCA                                                                                              <  SRR19973066.2392633/150‑1 (MQ=60)
                                                                  ATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTG                                                                                   <  SRR19973066.784287/150‑1 (MQ=60)
                                                                  ATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTG                                                                                   <  SRR19973066.2583296/150‑1 (MQ=60)
                                                                  ATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTG                                                                                   >  SRR19973066.1154255/1‑150 (MQ=60)
                                                                  ATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTG                                                                                   >  SRR19973066.1679837/1‑150 (MQ=60)
                                                                    GCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGT                                                                                 >  SRR19973066.3431166/1‑150 (MQ=60)
                                                                    GCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGT                                                                                 >  SRR19973066.1654384/1‑150 (MQ=60)
                                                                    GCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAAATGCTCCGCCAGCGTTGTTTCATCAAACGCAAAACCGACTGCGTTGGCAATACCCagaaccaacggacagctggt                                                                                 >  SRR19973066.2412318/1‑130 (MQ=60)
                                                                     CCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTG                                                                                >  SRR19973066.761585/1‑150 (MQ=60)
                                                                     CCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTG                                                                                >  SRR19973066.2561250/1‑150 (MQ=60)
                                                                     CCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTG                                                                                >  SRR19973066.1350135/1‑150 (MQ=60)
                                                                      CTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGG                                                                               >  SRR19973066.1358440/1‑150 (MQ=60)
                                                                       TTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGT                                                                              <  SRR19973066.1050784/150‑1 (MQ=60)
                                                                       TTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGT                                                                              <  SRR19973066.1050509/150‑1 (MQ=60)
                                                                        TCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTT                                                                             <  SRR19973066.604347/150‑1 (MQ=60)
                                                                           ATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCC                                                                          >  SRR19973066.3394613/1‑150 (MQ=60)
                                                                             CATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCAC                                                                        <  SRR19973066.2364874/150‑1 (MQ=60)
                                                                                 CAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCA                                                                    >  SRR19973066.990677/1‑150 (MQ=60)
                                                                                 CAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCA                                                                    >  SRR19973066.991089/1‑150 (MQ=60)
                                                                                  AGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAG                                                                   >  SRR19973066.2814239/1‑150 (MQ=60)
                                                                                     CGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGG                                                                >  SRR19973066.642185/1‑150 (MQ=60)
                                                                                     CGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGG                                                                >  SRR19973066.1784943/1‑150 (MQ=60)
                                                                                     CGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGG                                                                >  SRR19973066.3579284/1‑150 (MQ=60)
                                                                                           CCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAAC                                                          >  SRR19973066.620705/1‑150 (MQ=60)
                                                                                            CATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACC                                                         <  SRR19973066.3151558/150‑1 (MQ=60)
                                                                                            CATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACC                                                         <  SRR19973066.3151555/150‑1 (MQ=60)
                                                                                             ATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCC                                                        >  SRR19973066.1214325/1‑150 (MQ=60)
                                                                                                AAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTTGTGGTTTCCACACCAGCGGTGTAACCCACT                                                     >  SRR19973066.2662420/1‑150 (MQ=60)
                                                                                                AAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACT                                                     <  SRR19973066.716801/150‑1 (MQ=60)
                                                                                                AAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACT                                                     <  SRR19973066.716799/150‑1 (MQ=60)
                                                                                                AAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACT                                                     <  SRR19973066.2347541/150‑1 (MQ=60)
                                                                                                  GGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTC                                                   >  SRR19973066.209774/1‑150 (MQ=60)
                                                                                                    CGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCG                                                 <  SRR19973066.860842/150‑1 (MQ=60)
                                                                                                      TAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGG                                               >  SRR19973066.2325827/1‑150 (MQ=60)
                                                                                                      TAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGG                                               >  SRR19973066.880742/1‑150 (MQ=60)
                                                                                                      TAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGG                                               >  SRR19973066.880221/1‑150 (MQ=60)
                                                                                                      TAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGG                                               >  SRR19973066.2766182/1‑150 (MQ=60)
                                                                                                      TAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGG                                               >  SRR19973066.2325843/1‑150 (MQ=60)
                                                                                                      TAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGG                                               >  SRR19973066.1854196/1‑150 (MQ=60)
                                                                                                      TAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGG                                               >  SRR19973066.1856966/1‑150 (MQ=60)
                                                                                                       AGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGT                                              >  SRR19973066.95497/1‑150 (MQ=60)
                                                                                                       AGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGT                                              >  SRR19973066.95812/1‑150 (MQ=60)
                                                                                                            TAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCC                                         <  SRR19973066.2202529/150‑1 (MQ=60)
                                                                                                             AGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCG                                        >  SRR19973066.3327825/1‑150 (MQ=60)
                                                                                                             AGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCG                                        >  SRR19973066.2055112/1‑150 (MQ=60)
                                                                                                              GTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGG                                       >  SRR19973066.3327963/1‑150 (MQ=60)
                                                                                                               TGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGA                                      <  SRR19973066.3129404/150‑1 (MQ=60)
                                                                                                               TGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGA                                      <  SRR19973066.788917/150‑1 (MQ=60)
                                                                                                                 GTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGcgg                                    >  SRR19973066.1352703/1‑147 (MQ=60)
                                                                                                                 GTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGT                                    >  SRR19973066.3137515/1‑150 (MQ=60)
                                                                                                                 GTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGT                                    <  SRR19973066.3463287/150‑1 (MQ=60)
                                                                                                                 GTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGT                                    <  SRR19973066.2067262/150‑1 (MQ=60)
                                                                                                                 GTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCAGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTACCGGGTGACCCGGAGT                                    >  SRR19973066.867074/1‑150 (MQ=60)
                                                                                                                     ACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTA                                <  SRR19973066.2306099/150‑1 (MQ=60)
                                                                                                                     ACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTA                                <  SRR19973066.2305845/150‑1 (MQ=60)
                                                                                                                      CAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTTCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAG                               >  SRR19973066.219676/1‑150 (MQ=60)
                                                                                                                      CAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTGTTAG                               >  SRR19973066.439943/1‑150 (MQ=60)
                                                                                                                        ATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAG                             >  SRR19973066.480253/1‑150 (MQ=60)
                                                                                                                         TGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGT                            <  SRR19973066.2340144/150‑1 (MQ=60)
                                                                                                                            CGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCA                         >  SRR19973066.3128767/1‑150 (MQ=60)
                                                                                                                            CGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCA                         <  SRR19973066.1889946/150‑1 (MQ=60)
                                                                                                                                GCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATCCCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTG                     <  SRR19973066.2708520/150‑1 (MQ=60)
                                                                                                                                GCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTG                     <  SRR19973066.3074894/150‑1 (MQ=60)
                                                                                                                                  CCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGAC                   <  SRR19973066.1474900/150‑1 (MQ=60)
                                                                                                                                  CCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGAC                   <  SRR19973066.1474250/150‑1 (MQ=60)
                                                                                                                                  CCGGACGGTTAAAATGCTCCGCCAGCGTTTTTTCTTCAATCGCCATACCGACTGCGTTTTCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGAC                   <  SRR19973066.1474907/150‑1 (MQ=60)
                                                                                                                                   CGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACG                  >  SRR19973066.934231/1‑150 (MQ=60)
                                                                                                                                   CGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACG                  <  SRR19973066.3319862/150‑1 (MQ=60)
                                                                                                                                   CGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTGTAGAGTGCAGCTGACG                  >  SRR19973066.956858/1‑150 (MQ=60)
                                                                                                                                       CGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAG              <  SRR19973066.1962608/150‑1 (MQ=60)
                                                                                                                                           TAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTT          >  SRR19973066.2786298/1‑150 (MQ=60)
                                                                                                                                           TAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTT          >  SRR19973066.1568078/1‑150 (MQ=60)
                                                                                                                                           TAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTT          >  SRR19973066.1568164/1‑150 (MQ=60)
                                                                                                                                           TAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGGGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTT          >  SRR19973066.2786157/1‑150 (MQ=60)
                                                                                                                                             AACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTC        >  SRR19973066.1020785/1‑150 (MQ=60)
                                                                                                                                               CTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAG      <  SRR19973066.1259519/150‑1 (MQ=60)
                                                                                                                                                  CTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACATCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTC   >  SRR19973066.423181/1‑150 (MQ=60)
                                                                                                                                                   TCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCT  <  SRR19973066.332921/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
TCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCT  >  CP009273/3074444‑3074740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑