Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,074,591 G→T 100% A135E (GCG→GAG)  tktA ← transketolase 1, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,074,5910GT98.8% 538.8 / ‑2.8 168A135E (GCG→GAG) tktAtransketolase 1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (89/77);  total (89/79)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 171 total aligned reads displayed.

ATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTT  >  CP009273/3074446‑3074733
                                                                                                                                                 |                                                                                                                                              
  cGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGctccgc                                                                                                                                            <  2:181811/148‑1 (MQ=255)
       aaTGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCg                                                                                                                                       <  2:1641021/148‑1 (MQ=255)
       aaTGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCg                                                                                                                                       <  1:1990242/148‑1 (MQ=255)
        aTGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGt                                                                                                                                      >  2:192453/1‑148 (MQ=255)
        aTGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGGAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGt                                                                                                                                      >  1:3206446/1‑148 (MQ=255)
          gCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGttt                                                                                                                                    <  2:1549336/148‑1 (MQ=255)
           cAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGtttt                                                                                                                                   >  2:901559/1‑148 (MQ=255)
             aTCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGtttttt                                                                                                                                 >  2:1250460/1‑148 (MQ=255)
             aTCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGtttttt                                                                                                                                 <  2:2494552/148‑1 (MQ=255)
             aTCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGtttttt                                                                                                                                 <  2:1593056/148‑1 (MQ=255)
              tCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTc                                                                                                                                >  1:391499/1‑148 (MQ=255)
              tCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTc                                                                                                                                >  2:2357755/1‑148 (MQ=255)
               cAGTTTACCCAGCTTCAGAGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCAGTCTCCCATGAATGCGTAGGTGTAGTGGTCGACAATGTCGTGTCCCGGACGGTTAATCTGCTCCGCCAGCGTTTTTTCt                                                                                                                               >  2:1209397/1‑148 (MQ=255)
                    tACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAAt                                                                                                                          <  1:92416/148‑1 (MQ=255)
                         aGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCa                                                                                                                     >  2:1523276/1‑148 (MQ=255)
                           cTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATa                                                                                                                   <  2:147704/148‑1 (MQ=255)
                            ttCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATAc                                                                                                                  <  2:1631206/148‑1 (MQ=255)
                             tCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATAcc                                                                                                                 >  1:181272/1‑148 (MQ=255)
                                 cGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACt                                                                                                             >  1:1637346/1‑148 (MQ=255)
                                 cGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACt                                                                                                             >  2:2077659/1‑148 (MQ=255)
                                    aCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCg                                                                                                          >  2:1719954/1‑148 (MQ=255)
                                    aCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTGTTTCTGCAATCGCCATACCGACTGCg                                                                                                          >  2:1017827/1‑148 (MQ=255)
                                     cccGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGt                                                                                                         >  1:76088/1‑148 (MQ=255)
                                     cccGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGt                                                                                                         >  1:2336415/1‑148 (MQ=255)
                                         ccAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGc                                                                                                     >  1:1464394/1‑148 (MQ=255)
                                           agagagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCaa                                                                                                   >  1:79578/1‑148 (MQ=255)
                                             agagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATa                                                                                                 >  1:2584410/1‑148 (MQ=255)
                                             agagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATa                                                                                                 >  2:2499006/1‑148 (MQ=255)
                                             agagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATa                                                                                                 >  1:1597887/1‑148 (MQ=255)
                                             agagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTATGCAATCGCAAAAACGACTTCGATGGCaaaa                                                                                                 >  1:2073792/1‑146 (MQ=255)
                                              gagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATAc                                                                                                >  1:719590/1‑148 (MQ=255)
                                               agCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATAcc                                                                                               <  1:2065094/148‑1 (MQ=255)
                                                 cAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCt                                                                                             >  2:2131341/1‑148 (MQ=255)
                                                 cAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCt                                                                                             >  1:1342436/1‑148 (MQ=255)
                                                    aCTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAc                                                                                          >  1:2834209/1‑148 (MQ=255)
                                                     cTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAcc                                                                                         <  1:2984703/148‑1 (MQ=255)
                                                     cTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAcc                                                                                         <  1:1219054/148‑1 (MQ=255)
                                                       tCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCa                                                                                       >  2:3160917/1‑148 (MQ=255)
                                                            ggAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGa                                                                                  <  2:647897/148‑1 (MQ=255)
                                                            ggAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGa                                                                                  >  2:2251163/1‑148 (MQ=255)
                                                            ggAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGa                                                                                  >  1:108189/1‑148 (MQ=255)
                                                            ggAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGGTGGCAATACCCTGACCCAGCGGa                                                                                  >  1:1965885/1‑148 (MQ=255)
                                                             gagaTGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGAc                                                                                 <  2:2394674/148‑1 (MQ=255)
                                                               gaTGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCg                                                                               <  2:684375/148‑1 (MQ=255)
                                                                  gCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCggtg                                                                            <  1:331287/148‑1 (MQ=255)
                                                                   ccTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCggtgg                                                                           <  1:1363201/148‑1 (MQ=255)
                                                                   ccTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTGTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCggtgg                                                                           <  2:2770757/148‑1 (MQ=255)
                                                                    cTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCggtggt                                                                          <  1:3515310/148‑1 (MQ=255)
                                                                      tCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCggtggtgg                                                                        <  2:2434576/148‑1 (MQ=255)
                                                                       ccATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCggtggtggt                                                                       <  2:425319/148‑1 (MQ=255)
                                                                           catGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTcc                                                                   >  1:1982548/1‑148 (MQ=255)
                                                                           catGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTcc                                                                   >  2:3392284/1‑148 (MQ=255)
                                                                                 gCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCa                                                             >  2:1445816/1‑148 (MQ=255)
                                                                                        cccATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGgtgt                                                      >  2:215572/1‑148 (MQ=255)
                                                                                          cATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTaa                                                    <  1:2357755/148‑1 (MQ=255)
                                                                                           aTGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCCCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAAc                                                   >  1:2647409/1‑148 (MQ=255)
                                                                                            tGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAAcc                                                  >  2:2048749/1‑148 (MQ=255)
                                                                                             gAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAACCAGCGGACCGGTGGTGTTTTCCACACCAGCGGTGTAAccc                                                 >  1:1892916/1‑148 (MQ=255)
                                                                                              aaGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCa                                                <  1:264546/148‑1 (MQ=255)
                                                                                              aaGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCa                                                <  1:2036594/148‑1 (MQ=255)
                                                                                               aGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCAc                                               >  2:2994221/1‑148 (MQ=255)
                                                                                                 gCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACtt                                             <  1:2443299/148‑1 (MQ=255)
                                                                                                     aGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCgg                                         <  2:66292/148‑1 (MQ=255)
                                                                                                     aGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCgg                                         <  2:1843903/148‑1 (MQ=255)
                                                                                                     aGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCgg                                         <  1:759773/148‑1 (MQ=255)
                                                                                                      ggTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCggg                                        >  2:47049/1‑148 (MQ=255)
                                                                                                       gtgtAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGt                                       <  1:1075054/148‑1 (MQ=255)
                                                                                                           aGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGAcc                                   <  2:3206446/148‑1 (MQ=255)
                                                                                                           aGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGAcc                                   >  1:1745207/1‑148 (MQ=255)
                                                                                                           aGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGAcc                                   >  1:3483499/1‑148 (MQ=255)
                                                                                                           aGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGAcc                                   <  1:623867/148‑1 (MQ=255)
                                                                                                               gTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGa                               <  2:391499/148‑1 (MQ=255)
                                                                                                               gTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGa                               <  2:2407424/148‑1 (MQ=255)
                                                                                                                 cGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGt                             <  1:1021282/148‑1 (MQ=255)
                                                                                                                 cGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGt                             <  1:3164310/148‑1 (MQ=255)
                                                                                                                 cGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGt                             <  2:1357721/148‑1 (MQ=255)
                                                                                                                 cGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGt                             <  1:1021030/148‑1 (MQ=255)
                                                                                                                  gACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGtt                            <  1:3243207/148‑1 (MQ=255)
                                                                                                                  gACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCAACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGtt                            <  1:2214052/148‑1 (MQ=255)
                                                                                                                   aCAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGttt                           >  2:2846842/1‑148 (MQ=255)
                                                                                                                    cAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGtttt                          >  1:502676/1‑148 (MQ=255)
                                                                                                                      aTGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTag                        <  2:2374042/148‑1 (MQ=255)
                                                                                                                        gTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTagag                      <  1:3489951/148‑1 (MQ=255)
                                                                                                                            tGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCa                  <  2:2600890/148‑1 (MQ=255)
                                                                                                                            tGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCa                  <  2:2031385/148‑1 (MQ=255)
                                                                                                                             ggCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAg                 <  2:194561/148‑1 (MQ=255)
                                                                                                                               cccGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCt               >  2:1635255/1‑148 (MQ=255)
                                                                                                                                 ccgACGGCGAAACCGCTCCGCCAGCGTTTTGTCTGCAATCGCCATACCGCCTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGa             <  2:3498866/146‑1 (MQ=255)
                                                                                                                                 cGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGa             >  1:1100470/1‑148 (MQ=255)
                                                                                                                                     cGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGa         <  2:872352/148‑1 (MQ=255)
                                                                                                                                     cGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGa         <  1:2077659/148‑1 (MQ=255)
                                                                                                                                      ggTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGaa        >  2:2015274/1‑148 (MQ=255)
                                                                                                                                      ggTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGaa        <  2:2340817/148‑1 (MQ=255)
                                                                                                                                       gTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAg       <  2:2589073/148‑1 (MQ=255)
                                                                                                                                       gTTAAACGGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAg       <  2:1891759/148‑1 (MQ=255)
                                                                                                                                        ttAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGt      >  1:2581919/1‑148 (MQ=255)
                                                                                                                                        ttAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGt      >  2:1785449/1‑148 (MQ=255)
                                                                                                                                          aaaCTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGttt    >  1:2116752/1‑148 (MQ=255)
                                                                                                                                            aCTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGttttt  >  1:193446/1‑148 (MQ=255)
                                                                                                                                            aCTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGttttt  >  2:3420096/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
ATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTT  >  CP009273/3074446‑3074733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTC  >  CP009273/3074444‑3074739
                                                                                                                                                   |                                                                                                                                                    
TCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCC                                                                                                                                                    >  SRR19973065.1757703/1‑150 (MQ=60)
     GTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAG                                                                                                                                               >  SRR19973065.2798566/1‑150 (MQ=60)
     GTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAG                                                                                                                                               >  SRR19973065.421900/1‑150 (MQ=60)
        GAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGGAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGT                                                                                                                                            >  SRR19973065.3225744/1‑150 (MQ=60)
         AATGCAATCAGTTTACCCTGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTT                                                                                                                                           <  SRR19973065.2002756/150‑1 (MQ=60)
         AATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTT                                                                                                                                           <  SRR19973065.2003152/150‑1 (MQ=60)
              AATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTC                                                                                                                                      >  SRR19973065.394234/1‑150 (MQ=60)
                    TTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAAT                                                                                                                                <  SRR19973065.2068230/150‑1 (MQ=60)
                     TTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATC                                                                                                                               <  SRR19973065.3498097/150‑1 (MQ=60)
                      TACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCG                                                                                                                              <  SRR19973065.93295/150‑1 (MQ=60)
                        CCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCC                                                                                                                            >  SRR19973065.372361/1‑150 (MQ=60)
                          CAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGGCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTGCTGCAATCGCCAT                                                                                                                          >  SRR19973065.1861626/1‑150 (MQ=60)
                             CTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACC                                                                                                                       >  SRR19973065.182706/1‑150 (MQ=60)
                                CAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGAC                                                                                                                    >  SRR19973065.3529135/1‑150 (MQ=60)
                                 AGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACT                                                                                                                   >  SRR19973065.1648339/1‑150 (MQ=60)
                                     TACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGT                                                                                                               >  SRR19973065.76710/1‑150 (MQ=60)
                                     TACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGT                                                                                                               >  SRR19973065.2351003/1‑150 (MQ=60)
                                     TACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGT                                                                                                               >  SRR19973065.3061538/1‑150 (MQ=60)
                                     TACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGT                                                                                                               >  SRR19973065.76858/1‑150 (MQ=60)
                                         CGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGC                                                                                                           >  SRR19973065.1474182/1‑150 (MQ=60)
                                         CGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGC                                                                                                           >  SRR19973065.1473797/1‑150 (MQ=60)
                                           CCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAA                                                                                                         >  SRR19973065.80379/1‑150 (MQ=60)
                                             AGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATA                                                                                                       >  SRR19973065.2600510/1‑150 (MQ=60)
                                             AGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATA                                                                                                       >  SRR19973065.1608558/1‑150 (MQ=60)
                                             AGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTATGCAATCGCaaaaacgacttcgatggcaaaa                                                                                                       >  SRR19973065.2087036/1‑128 (MQ=60)
                                              GAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATAC                                                                                                      >  SRR19973065.724407/1‑150 (MQ=60)
                                                 AGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCT                                                                                                   >  SRR19973065.196074/1‑150 (MQ=60)
                                                 AGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCT                                                                                                   <  SRR19973065.2116833/150‑1 (MQ=60)
                                                 AGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCT                                                                                                   >  SRR19973065.1351315/1‑150 (MQ=60)
                                                 AGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCT                                                                                                   <  SRR19973065.2078264/150‑1 (MQ=60)
                                                 AGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCT                                                                                                   <  SRR19973065.2078255/150‑1 (MQ=60)
                                                    AAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAC                                                                                                >  SRR19973065.2851669/1‑150 (MQ=60)
                                                       CTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCA                                                                                             <  SRR19973065.1226971/150‑1 (MQ=60)
                                                       CTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCA                                                                                             <  SRR19973065.3002974/150‑1 (MQ=60)
                                                            TGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGA                                                                                        >  SRR19973065.1978724/1‑150 (MQ=60)
                                                            TGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGA                                                                                        >  SRR19973065.109082/1‑150 (MQ=60)
                                                            TGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGA                                                                                        >  SRR19973065.109174/1‑150 (MQ=60)
                                                            TGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGGTGGCAATACCCTGACCCAGCGGA                                                                                        >  SRR19973065.1978716/1‑150 (MQ=60)
                                                                  ATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTG                                                                                  >  SRR19973065.2576137/1‑150 (MQ=60)
                                                                    GCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGT                                                                                <  SRR19973065.333607/150‑1 (MQ=60)
                                                                     CCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTG                                                                               <  SRR19973065.1372239/150‑1 (MQ=60)
                                                                     CCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTG                                                                               <  SRR19973065.1372221/150‑1 (MQ=60)
                                                                      CTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGG                                                                              <  SRR19973065.3536620/150‑1 (MQ=60)
                                                                          CATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTC                                                                          >  SRR19973065.1254488/1‑150 (MQ=60)
                                                                           ATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCC                                                                         >  SRR19973065.1995431/1‑150 (MQ=60)
                                                                                           CCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAAC                                                         >  SRR19973065.2663700/1‑150 (MQ=60)
                                                                                           CCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCCCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAAC                                                         >  SRR19973065.2663730/1‑150 (MQ=60)
                                                                                            CATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACC                                                        <  SRR19973065.2372411/150‑1 (MQ=60)
                                                                                             ATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCC                                                       <  SRR19973065.2235698/150‑1 (MQ=60)
                                                                                             ATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAACCAGCGGACCGGTGGTGTTTTCCACACCAGCGGTGTAACCC                                                       >  SRR19973065.1905359/1‑150 (MQ=60)
                                                                                                AAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACT                                                    <  SRR19973065.266293/150‑1 (MQ=60)
                                                                                                AAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACT                                                    <  SRR19973065.266357/150‑1 (MQ=60)
                                                                                                AAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACT                                                    <  SRR19973065.2049646/150‑1 (MQ=60)
                                                                                                 AGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTT                                                   <  SRR19973065.3191151/150‑1 (MQ=60)
                                                                                                   GCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCC                                                 <  SRR19973065.2458696/150‑1 (MQ=60)
                                                                                                       AGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGT                                             <  SRR19973065.764836/150‑1 (MQ=60)
                                                                                                         GTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGA                                           <  SRR19973065.1082161/150‑1 (MQ=60)
                                                                                                         GTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGA                                           <  SRR19973065.1081919/150‑1 (MQ=60)
                                                                                                           GTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACC                                         >  SRR19973065.3504694/1‑150 (MQ=60)
                                                                                                           GTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACC                                         >  SRR19973065.1756717/1‑150 (MQ=60)
                                                                                                           GTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTCTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACC                                         >  SRR19973065.911362/1‑150 (MQ=60)
                                                                                                             AGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCG                                       <  SRR19973065.627904/150‑1 (MQ=60)
                                                                                                             AGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCG                                       <  SRR19973065.628093/150‑1 (MQ=60)
                                                                                                                   CGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTT                                 <  SRR19973065.1028052/150‑1 (MQ=60)
                                                                                                                   CGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTT                                 <  SRR19973065.3183478/150‑1 (MQ=60)
                                                                                                                   CGACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTT                                 <  SRR19973065.1027798/150‑1 (MQ=60)
                                                                                                                    GACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTT                                <  SRR19973065.3262766/150‑1 (MQ=60)
                                                                                                                    GACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTT                                >  SRR19973065.506037/1‑150 (MQ=60)
                                                                                                                    GACAATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCAACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTT                                <  SRR19973065.2228204/150‑1 (MQ=60)
                                                                                                                    GACAAGGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTT                                <  SRR19973065.3263369/150‑1 (MQ=60)
                                                                                                                        ATGTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAG                            <  SRR19973065.2455336/150‑1 (MQ=60)
                                                                                                                          GTCGTGGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTG                          <  SRR19973065.3511175/150‑1 (MQ=60)
                                                                                                                               GGCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCT                     <  SRR19973065.115864/150‑1 (MQ=60)
                                                                                                                                GCCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTG                    <  SRR19973065.798879/150‑1 (MQ=60)
                                                                                                                                 CCCGGACGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGA                   >  SRR19973065.1107703/1‑150 (MQ=60)
                                                                                                                                       CGGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAG             <  SRR19973065.2090942/150‑1 (MQ=60)
                                                                                                                                        GGTTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGT            >  SRR19973065.2598014/1‑150 (MQ=60)
                                                                                                                                          TTAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTT          >  SRR19973065.2130374/1‑150 (MQ=60)
                                                                                                                                           TAAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTT         <  SRR19973065.161459/150‑1 (MQ=60)
                                                                                                                                            AAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTT        <  SRR19973065.217195/150‑1 (MQ=60)
                                                                                                                                            AAACTGCTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTT        >  SRR19973065.194953/1‑150 (MQ=60)
                                                                                                                                                  CTCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTC  >  SRR19973065.442240/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
TCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTC  >  CP009273/3074444‑3074739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑