Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,077,302 C→A 100% D657Y (GAT→TAT)  speA ← biosynthetic arginine decarboxylase, PLP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,077,3020CA96.0% 638.1 / 14.7 211D657Y (GAT→TAT) speAbiosynthetic arginine decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base C (4/5);  new base A (102/100);  total (106/105)
Fisher's exact test for biased strand distribution p-value = 7.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 211 total aligned reads displayed.

ATGGACTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCA  >  CP009273/3077159‑3077445
                                                                                                                                               |                                                                                                                                               
atggaCTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCa                                                                                                                                             >  2:2258657/1‑148 (MQ=255)
atggaCTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCa                                                                                                                                             >  2:90396/1‑148 (MQ=255)
   gaCTCCTATGACAAAGTCGATATCAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATGTGTCCCAATAAATTGTGGCGGATTAGCACCGCTAAATTAAGCGGATTCAAGGAACACAGGACTTACTCATATTCAAGa                                                                                                                                          <  2:1243988/148‑1 (MQ=255)
    aCTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGAt                                                                                                                                         >  2:1478438/1‑148 (MQ=255)
     ctacTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATa                                                                                                                                        <  2:2375577/145‑1 (MQ=255)
     cTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATa                                                                                                                                        >  2:1115895/1‑148 (MQ=255)
     cTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATa                                                                                                                                        >  2:417337/1‑148 (MQ=255)
       ccTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAg                                                                                                                                      <  1:1051753/148‑1 (MQ=255)
        cTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACGATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGt                                                                                                                                     <  2:1603260/148‑1 (MQ=255)
           ttACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGtata                                                                                                                                  <  2:2160297/148‑1 (MQ=255)
            tACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATaa                                                                                                                                 <  1:2692119/148‑1 (MQ=255)
             aCAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAAc                                                                                                                                <  1:3269893/148‑1 (MQ=255)
                aaGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGt                                                                                                                             >  1:215114/1‑148 (MQ=255)
                aaGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGt                                                                                                                             >  2:136381/1‑148 (MQ=255)
                   tCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACa                                                                                                                          <  1:833812/148‑1 (MQ=255)
                    cGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACaa                                                                                                                         >  2:682787/1‑148 (MQ=255)
                     gATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACaaa                                                                                                                        <  2:2939525/148‑1 (MQ=255)
                       tataAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAAcc                                                                                                                      >  2:1169148/1‑148 (MQ=255)
                       tataAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAAcc                                                                                                                      >  1:3052378/1‑148 (MQ=255)
                       tataAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAAcc                                                                                                                      >  2:589055/1‑148 (MQ=255)
                              aaaCCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCg                                                                                                               >  2:3093478/1‑148 (MQ=255)
                               aaCCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGa                                                                                                              <  2:1004401/148‑1 (MQ=255)
                                aCCCTTCCTCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGaa                                                                                                             <  1:1336851/148‑1 (MQ=255)
                                 cccTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAAc                                                                                                            >  2:1900613/1‑148 (MQ=255)
                                 cccGTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAAc                                                                                                            <  2:1818509/148‑1 (MQ=255)
                                    ttCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAActct                                                                                                         <  1:449593/148‑1 (MQ=255)
                                          gtcgtTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAgg                                                                                                   <  2:141701/148‑1 (MQ=255)
                                             gtTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAAc                                                                                                >  1:357707/1‑148 (MQ=255)
                                               tAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtg                                                                                              >  1:1385035/1‑148 (MQ=255)
                                               tAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtg                                                                                              <  1:722682/148‑1 (MQ=255)
                                                   cccGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtgttgt                                                                                          <  1:3028408/148‑1 (MQ=255)
                                                         gaGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGt                                                                                    <  1:3392761/148‑1 (MQ=255)
                                                              aGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGc                                                                               >  1:2002910/1‑148 (MQ=255)
                                                              aGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGc                                                                               >  1:1369556/1‑148 (MQ=255)
                                                                ggTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCAt                                                                             <  1:999775/148‑1 (MQ=255)
                                                                ggTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCAt                                                                             <  1:299864/148‑1 (MQ=255)
                                                                  ttGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCa                                                                           <  2:405804/148‑1 (MQ=255)
                                                                  ttGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCa                                                                           >  1:1144616/1‑148 (MQ=255)
                                                                   tGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCaa                                                                          >  1:2841262/1‑148 (MQ=255)
                                                                   tGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCaa                                                                          <  2:672960/148‑1 (MQ=255)
                                                                   tGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCaa                                                                          >  1:2617991/1‑148 (MQ=255)
                                                                     gATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGa                                                                        >  2:1763674/1‑148 (MQ=255)
                                                                      aTTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGAt                                                                       >  2:1562779/1‑148 (MQ=255)
                                                                       tttGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATc                                                                      <  1:1115895/148‑1 (MQ=255)
                                                                       tttGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATc                                                                      >  1:179216/1‑148 (MQ=255)
                                                                        ttGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCg                                                                     >  2:2116322/1‑148 (MQ=255)
                                                                        ttGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCg                                                                     >  2:324502/1‑148 (MQ=255)
                                                                           tCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGtt                                                                  >  1:1025363/1‑148 (MQ=255)
                                                                               aaTAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTttct                                                              <  2:618544/148‑1 (MQ=255)
                                                                                aTAAATTGTGGCGGATTAACACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAATTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTttctt                                                             >  1:1502973/1‑148 (MQ=255)
                                                                                 tAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTttcttc                                                            >  1:616327/1‑148 (MQ=255)
                                                                                  aaaTTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTCACACAGGACTTACTCATATTCAAGCTAAGTAGAACCGTACAAACCTGCCTCGAACTCTTCAAGGTACTGTTTTTGCAGTTCAGCATCAAGATCGGTTTTCTTCa                                                           <  2:1864061/148‑1 (MQ=255)
                                                                                  aaaTTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCa                                                           >  2:2269235/1‑148 (MQ=255)
                                                                                  aaaTTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGCACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCa                                                           >  1:2181019/1‑148 (MQ=255)
                                                                                   aaTTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCAc                                                          >  2:1331215/1‑148 (MQ=255)
                                                                                     ttGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACtt                                                        <  2:780454/148‑1 (MQ=255)
                                                                                       gtgGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGa                                                      <  1:1612096/148‑1 (MQ=255)
                                                                                       gtgGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGa                                                      <  2:775494/148‑1 (MQ=255)
                                                                                       gtgGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGa                                                      <  2:2750484/148‑1 (MQ=255)
                                                                                           cGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATcgc                                                  <  1:90396/148‑1 (MQ=255)
                                                                                           cGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATcgc                                                  <  1:90428/148‑1 (MQ=255)
                                                                                             gATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCgg                                                >  1:2254512/1‑148 (MQ=255)
                                                                                              aTTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGa                                               >  1:3138518/1‑148 (MQ=255)
                                                                                              aTTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGa                                               <  2:3295840/148‑1 (MQ=255)
                                                                                                   cACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTgg                                          >  2:905392/1‑148 (MQ=255)
                                                                                                   cACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTgg                                          >  2:650368/1‑148 (MQ=255)
                                                                                                     ccGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGt                                        <  1:2227134/148‑1 (MQ=255)
                                                                                                     ccGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGt                                        <  1:326277/148‑1 (MQ=255)
                                                                                                      cGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGtt                                       <  2:2782457/148‑1 (MQ=255)
                                                                                                       gCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTa                                      >  1:1557933/1‑148 (MQ=255)
                                                                                                        cTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGGACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTaa                                     <  1:1087071/148‑1 (MQ=255)
                                                                                                         tAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAAc                                    <  2:1379608/148‑1 (MQ=255)
                                                                                                          aaaTTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACa                                   >  2:2495472/1‑148 (MQ=255)
                                                                                                           aaTTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAg                                  >  1:2281836/1‑148 (MQ=255)
                                                                                                            aTTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGc                                 >  1:2417013/1‑148 (MQ=255)
                                                                                                             ttAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCg                                >  1:1687966/1‑148 (MQ=255)
                                                                                                               aaGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGtt                              <  2:198680/148‑1 (MQ=255)
                                                                                                               aaGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGtt                              <  1:2303387/148‑1 (MQ=255)
                                                                                                                aGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGttt                             <  2:1959096/148‑1 (MQ=255)
                                                                                                                 gCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGtttt                            >  2:3023862/1‑148 (MQ=255)
                                                                                                                  cGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTc                           >  1:1207809/1‑148 (MQ=255)
                                                                                                                    gATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCgg                         >  2:3465226/1‑148 (MQ=255)
                                                                                                                    gATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCgg                         >  2:3464821/1‑148 (MQ=255)
                                                                                                                     aTTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGa                        <  2:117791/148‑1 (MQ=255)
                                                                                                                          aGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAg                   <  2:267515/148‑1 (MQ=255)
                                                                                                                          aGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAg                   <  2:2617991/148‑1 (MQ=255)
                                                                                                                            tAACACAGGACTGACTCATATTCAAGCTAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCCAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCt                 <  2:2823544/148‑1 (MQ=255)
                                                                                                                              acacAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGt               >  2:1285845/1‑148 (MQ=255)
                                                                                                                              acacAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGt               >  2:2637150/1‑148 (MQ=255)
                                                                                                                              acacAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGt               >  1:3424412/1‑148 (MQ=255)
                                                                                                                               cacaGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTa              >  2:230669/1‑148 (MQ=255)
                                                                                                                                acaGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTAc             <  1:1235274/148‑1 (MQ=255)
                                                                                                                                 caGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACa            >  2:354255/1‑148 (MQ=255)
                                                                                                                                   ggACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACata          >  2:1992553/1‑148 (MQ=255)
                                                                                                                                   ggACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACata          >  2:471324/1‑148 (MQ=255)
                                                                                                                                   ggACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACata          >  1:3444112/1‑148 (MQ=255)
                                                                                                                                        tACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTgca     <  1:1900613/148‑1 (MQ=255)
                                                                                                                                        tACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTgca     >  2:1629913/1‑148 (MQ=255)
                                                                                                                                          cTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTgcagc   >  1:2446362/1‑148 (MQ=255)
                                                                                                                                          cTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTgcagc   >  2:3274862/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                               
ATGGACTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCA  >  CP009273/3077159‑3077445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCG  >  CP009273/3077164‑3077451
                                                                                                                                          |                                                                                                                                                     
CTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAG                                                                                                                                            <  SRR19973067.3185836/150‑1 (MQ=60)
CTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAG                                                                                                                                            >  SRR19973067.1311979/1‑150 (MQ=60)
  CCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTA                                                                                                                                          <  SRR19973067.1058967/150‑1 (MQ=60)
  CCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTA                                                                                                                                          <  SRR19973067.1059200/150‑1 (MQ=60)
   CTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTAT                                                                                                                                         >  SRR19973067.2947290/1‑150 (MQ=60)
       TACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACC                                                                                                                                     <  SRR19973067.2709421/150‑1 (MQ=60)
        ACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCG                                                                                                                                    <  SRR19973067.3290314/150‑1 (MQ=60)
         CAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGT                                                                                                                                   <  SRR19973067.2799524/150‑1 (MQ=60)
         CAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGT                                                                                                                                   >  SRR19973067.217816/1‑150 (MQ=60)
         CAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGT                                                                                                                                   >  SRR19973067.216705/1‑150 (MQ=60)
           AAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTAC                                                                                                                                 <  SRR19973067.957832/150‑1 (MQ=60)
             GTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAA                                                                                                                               >  SRR19973067.2488669/1‑150 (MQ=60)
              TCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAA                                                                                                                              <  SRR19973067.839486/150‑1 (MQ=60)
                GATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACC                                                                                                                            >  SRR19973067.3071827/1‑150 (MQ=60)
                    TAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCC                                                                                                                        >  SRR19973067.2438093/1‑150 (MQ=60)
                         AAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAA                                                                                                                   >  SRR19973067.2299706/1‑150 (MQ=60)
                           ACCCTTCCTCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACT                                                                                                                 <  SRR19973067.1345998/150‑1 (MQ=60)
                               TTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTC                                                                                                             <  SRR19973067.452714/150‑1 (MQ=60)
                                TCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCA                                                                                                            <  SRR19973067.3454113/150‑1 (MQ=60)
                                      TCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAAC                                                                                                      >  SRR19973067.360188/1‑150 (MQ=60)
                                      TCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAAC                                                                                                      >  SRR19973067.359599/1‑150 (MQ=60)
                                        GTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTG                                                                                                    >  SRR19973067.1394289/1‑150 (MQ=60)
                                        GTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTG                                                                                                    >  SRR19973067.1394462/1‑150 (MQ=60)
                                          TAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTT                                                                                                  <  SRR19973067.727705/150‑1 (MQ=60)
                                              CCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTG                                                                                              <  SRR19973067.3047919/150‑1 (MQ=60)
                                              CCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTG                                                                                              <  SRR19973067.3047688/150‑1 (MQ=60)
                                                  ACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGT                                                                                          <  SRR19973067.2062868/150‑1 (MQ=60)
                                                    GAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTC                                                                                        <  SRR19973067.3414346/150‑1 (MQ=60)
                                                      GGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAG                                                                                      >  SRR19973067.1203807/1‑150 (MQ=60)
                                                       GAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGC                                                                                     >  SRR19973067.1378909/1‑150 (MQ=60)
                                                       GAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGC                                                                                     >  SRR19973067.2016410/1‑150 (MQ=60)
                                                          GGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATC                                                                                  <  SRR19973067.720171/150‑1 (MQ=60)
                                                           GGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCC                                                                                 <  SRR19973067.1006647/150‑1 (MQ=60)
                                                           GGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCA                                                                                 <  SRR19973067.301778/150‑1 (MQ=60)
                                                           GGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCA                                                                                 >  SRR19973067.1152292/1‑150 (MQ=60)
                                                            GTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAA                                                                                >  SRR19973067.2859506/1‑150 (MQ=60)
                                                            GTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAA                                                                                >  SRR19973067.2634971/1‑150 (MQ=60)
                                                               GGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGAT                                                                             >  SRR19973067.1942150/1‑150 (MQ=60)
                                                               GGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGAT                                                                             <  SRR19973067.3021089/150‑1 (MQ=60)
                                                                GATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATC                                                                            >  SRR19973067.180642/1‑150 (MQ=60)
                                                                GATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATC                                                                            >  SRR19973067.114196/1‑150 (MQ=60)
                                                                 ATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCG                                                                           <  SRR19973067.177734/150‑1 (MQ=60)
                                                                  TTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGG                                                                          <  SRR19973067.1123464/150‑1 (MQ=60)
                                                                  TTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGG                                                                          <  SRR19973067.1123421/150‑1 (MQ=60)
                                                                    TGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTT                                                                        >  SRR19973067.1032416/1‑150 (MQ=60)
                                                                    TGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTT                                                                        >  SRR19973067.1032428/1‑150 (MQ=60)
                                                                         CAATAAATTGTGGCGGATTAACACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAATTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTT                                                                   >  SRR19973067.1512866/1‑150 (MQ=60)
                                                                          AATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTC                                                                  >  SRR19973067.620360/1‑150 (MQ=60)
                                                                          AATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTC                                                                  >  SRR19973067.2235684/1‑150 (MQ=60)
                                                                           ATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGCACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCA                                                                 >  SRR19973067.2195604/1‑150 (MQ=60)
                                                                            TAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCAC                                                                >  SRR19973067.910178/1‑150 (MQ=60)
                                                                                TTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGA                                                            <  SRR19973067.3568582/150‑1 (MQ=60)
                                                                                  GTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATC                                                          <  SRR19973067.1622986/150‑1 (MQ=60)
                                                                                  GTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATC                                                          <  SRR19973067.1623165/150‑1 (MQ=60)
                                                                                  GTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATC                                                          <  SRR19973067.1623171/150‑1 (MQ=60)
                                                                                      CGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGG                                                      <  SRR19973067.2273643/150‑1 (MQ=60)
                                                                                      CGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGG                                                      <  SRR19973067.91394/150‑1 (MQ=60)
                                                                                      CGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGG                                                      <  SRR19973067.91362/150‑1 (MQ=60)
                                                                                      CGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGG                                                      >  SRR19973067.2269477/1‑150 (MQ=60)
                                                                                       GGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGA                                                     >  SRR19973067.3158448/1‑150 (MQ=60)
                                                                                                CCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTA                                            <  SRR19973067.2241573/150‑1 (MQ=60)
                                                                                                CCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTA                                            >  SRR19973067.1568435/1‑150 (MQ=60)
                                                                                                CCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTA                                            <  SRR19973067.328726/150‑1 (MQ=60)
                                                                                                CCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTA                                            <  SRR19973067.328503/150‑1 (MQ=60)
                                                                                                CCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTA                                            <  SRR19973067.2241960/150‑1 (MQ=60)
                                                                                                CCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTA                                            <  SRR19973067.2242381/150‑1 (MQ=60)
                                                                                                CCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTA                                            >  SRR19973067.1568554/1‑150 (MQ=60)
                                                                                                   CTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGGACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACA                                         <  SRR19973067.1094503/150‑1 (MQ=60)
                                                                                                    TAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAG                                        >  SRR19973067.2296925/1‑150 (MQ=60)
                                                                                                    TAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAG                                        <  SRR19973067.2926540/150‑1 (MQ=60)
                                                                                                     AAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGC                                       >  SRR19973067.2432845/1‑150 (MQ=60)
                                                                                                     AAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGC                                       >  SRR19973067.3423756/1‑150 (MQ=60)
                                                                                                      AATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCG                                      >  SRR19973067.1699594/1‑150 (MQ=60)
                                                                                                      AATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCG                                      >  SRR19973067.3145624/1‑150 (MQ=60)
                                                                                                          AAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTT                                  <  SRR19973067.2318564/150‑1 (MQ=60)
                                                                                                          AAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTT                                  <  SRR19973067.2318587/150‑1 (MQ=60)
                                                                                                           AGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTC                                 >  SRR19973067.1215738/1‑150 (MQ=60)
                                                                                                           AGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTC                                 >  SRR19973067.1215798/1‑150 (MQ=60)
                                                                                                             CGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGG                               >  SRR19973067.1374979/1‑150 (MQ=60)
                                                                                                                    AAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGC                        <  SRR19973067.2708386/150‑1 (MQ=60)
                                                                                                                     AGTAACACAGGACTTACTCATATTCAAGATAATTATAACCGTACAAAAATGCCTCGAACTCTTCAAGGAACAGTTGTAGCAGTTCAGCATAAAGAACGGTTTTCTTCACTTGATCGCGGAACTCGGTTAACAGCGATGTCGGATCGAGCT                       >  SRR19973067.1399349/1‑150 (MQ=60)
                                                                                                                     AGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCT                       >  SRR19973067.42606/1‑150 (MQ=60)
                                                                                                                     AGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCT                       <  SRR19973067.806700/150‑1 (MQ=60)
                                                                                                                       TAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGT                     >  SRR19973067.3446206/1‑150 (MQ=60)
                                                                                                                       TAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAAATGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGT                     >  SRR19973067.860088/1‑150 (MQ=60)
                                                                                                                           ACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACAT                 <  SRR19973067.1243562/150‑1 (MQ=60)
                                                                                                                            CAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATA                >  SRR19973067.3466028/1‑150 (MQ=60)
                                                                                                                            CAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGNTCAGCATGAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATA                >  SRR19973067.1818832/1‑150 (MQ=60)
                                                                                                                               GACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTG             <  SRR19973067.616127/150‑1 (MQ=60)
                                                                                                                               GACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTG             >  SRR19973067.3308179/1‑150 (MQ=60)
                                                                                                                                   TACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGC         <  SRR19973067.1913572/150‑1 (MQ=60)
                                                                                                                                   TACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGC         >  SRR19973067.2462433/1‑150 (MQ=60)
                                                                                                                                      TCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATG      >  SRR19973067.3341438/1‑150 (MQ=60)
                                                                                                                                      TCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATG      >  SRR19973067.3341230/1‑150 (MQ=60)
                                                                                                                                      TCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATG      <  SRR19973067.1488261/150‑1 (MQ=60)
                                                                                                                                        ATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTC    >  SRR19973067.341708/1‑150 (MQ=60)
                                                                                                                                        ATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTC    <  SRR19973067.2851661/150‑1 (MQ=60)
                                                                                                                                          ATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCG  >  SRR19973067.2335219/1‑150 (MQ=60)
                                                                                                                                          |                                                                                                                                                     
CTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCG  >  CP009273/3077164‑3077451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑