Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,077,302 C→A 100% D657Y (GAT→TAT)  speA ← biosynthetic arginine decarboxylase, PLP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,077,3020CA97.0% 614.4 / 8.8 203D657Y (GAT→TAT) speAbiosynthetic arginine decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base A (98/99);  total (101/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 203 total aligned reads displayed.

GATGGACTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCA  >  CP009273/3077158‑3077445
                                                                                                                                                |                                                                                                                                               
gatggaCTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTc                                                                                                                                              >  2:1608114/1‑148 (MQ=255)
 atggaCTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCa                                                                                                                                             <  2:2796199/148‑1 (MQ=255)
   ggaCTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAg                                                                                                                                           >  1:3356185/1‑148 (MQ=255)
   ggaCTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAg                                                                                                                                           <  1:1736471/148‑1 (MQ=255)
      cTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATa                                                                                                                                        >  1:786276/1‑148 (MQ=255)
       tCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATaa                                                                                                                                       <  2:3005419/148‑1 (MQ=255)
        ccTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAg                                                                                                                                      >  2:1086946/1‑148 (MQ=255)
          tATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGta                                                                                                                                    <  1:3182234/148‑1 (MQ=255)
             tACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGAATTACTCATATTCAAGATAAGTATaa                                                                                                                                 <  1:762753/148‑1 (MQ=255)
              aCAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAAc                                                                                                                                <  2:1259300/148‑1 (MQ=255)
                 aaGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGt                                                                                                                             >  1:911039/1‑148 (MQ=255)
                  aGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTa                                                                                                                            <  1:1155114/148‑1 (MQ=255)
                      gATCTAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACaaa                                                                                                                        <  2:1111006/148‑1 (MQ=255)
                      gATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACaaa                                                                                                                        <  1:2545266/148‑1 (MQ=255)
                       atatAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAAc                                                                                                                       >  1:1897883/1‑148 (MQ=255)
                        tataAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAAcc                                                                                                                      >  2:2815454/1‑148 (MQ=255)
                        tataAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAAcc                                                                                                                      <  2:2590996/148‑1 (MQ=255)
                         ataAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCt                                                                                                                     >  1:3510289/1‑148 (MQ=255)
                              aaaaCCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTc                                                                                                                >  1:606396/1‑148 (MQ=255)
                               aaaCCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCg                                                                                                               >  1:2675742/1‑148 (MQ=255)
                                aaCCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGTTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGa                                                                                                              >  1:2882750/1‑148 (MQ=255)
                                aaCCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGTTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGa                                                                                                              >  1:2495222/1‑148 (MQ=255)
                                 aCCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGaa                                                                                                             >  1:2755476/1‑148 (MQ=255)
                                 aCCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGaa                                                                                                             >  1:906823/1‑148 (MQ=255)
                                  cccTTCCGCGTCGTTAGGCCCGACGAGGACGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAAc                                                                                                            >  2:3360655/1‑148 (MQ=255)
                                  cccTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAAc                                                                                                            >  2:3355892/1‑148 (MQ=255)
                                   ccTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAAct                                                                                                           <  1:3015406/148‑1 (MQ=255)
                                     ttCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAActct                                                                                                         <  1:1437251/148‑1 (MQ=255)
                                     ttCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAActct                                                                                                         >  2:2096058/1‑148 (MQ=255)
                                     ttCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAActct                                                                                                         >  1:3555484/1‑148 (MQ=255)
                                     ttCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCACTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAActct                                                                                                         >  1:2282852/1‑148 (MQ=255)
                                       ccGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTc                                                                                                       <  1:2426289/148‑1 (MQ=255)
                                           gtcgtTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAgg                                                                                                   <  1:351975/148‑1 (MQ=255)
                                            tcgtTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGa                                                                                                  >  2:422180/1‑148 (MQ=255)
                                               ttAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACt                                                                                               <  1:2137065/148‑1 (MQ=255)
                                               ttAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACt                                                                                               >  1:1187321/1‑148 (MQ=255)
                                                tAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtg                                                                                              >  1:878388/1‑148 (MQ=255)
                                                tAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtg                                                                                              >  2:417045/1‑148 (MQ=255)
                                                    cccGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtgttgt                                                                                          >  2:327217/1‑148 (MQ=255)
                                                        acgaGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCa                                                                                      <  2:3087413/148‑1 (MQ=255)
                                                           ttgtaGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGtt                                                                                   <  2:2058430/144‑1 (MQ=255)
                                                                gggTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCa                                                                              >  2:835745/1‑148 (MQ=255)
                                                                   ttGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCa                                                                           >  1:3470830/1‑148 (MQ=255)
                                                                      gATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGa                                                                        <  2:1825988/148‑1 (MQ=255)
                                                                      gATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGa                                                                        >  1:3147229/1‑148 (MQ=255)
                                                                       aTTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGAt                                                                       >  1:1770321/1‑148 (MQ=255)
                                                                          tGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCgg                                                                    >  2:1515289/1‑148 (MQ=255)
                                                                          tGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCgg                                                                    >  2:2832275/1‑148 (MQ=255)
                                                                          tGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCgg                                                                    <  2:3460334/148‑1 (MQ=255)
                                                                           gTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGt                                                                   <  1:1874462/148‑1 (MQ=255)
                                                                            tCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGtt                                                                  <  2:625803/148‑1 (MQ=255)
                                                                             cacaATAACTTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGttt                                                                 <  2:2209283/148‑1 (MQ=255)
                                                                                aaTAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTttct                                                              <  2:758228/148‑1 (MQ=255)
                                                                                  tAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTttcttc                                                            >  1:1696375/1‑148 (MQ=255)
                                                                                   aaaTTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCa                                                           >  1:531385/1‑148 (MQ=255)
                                                                                    aaTTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCAc                                                          <  2:2242235/148‑1 (MQ=255)
                                                                                    aaTTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCAc                                                          <  2:3152085/148‑1 (MQ=255)
                                                                                      ttGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACtt                                                        <  2:1862457/148‑1 (MQ=255)
                                                                                      ttGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACtt                                                        <  2:3012788/148‑1 (MQ=255)
                                                                                       tgtgGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTg                                                       >  2:2489970/1‑148 (MQ=255)
                                                                                        gtgGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGa                                                      <  2:295777/148‑1 (MQ=255)
                                                                                        gtgGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGa                                                      <  1:417045/148‑1 (MQ=255)
                                                                                            cGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATcgc                                                  <  2:2783716/148‑1 (MQ=255)
                                                                                                 tATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAAc                                             >  2:3266208/1‑148 (MQ=255)
                                                                                                   tCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTg                                           >  2:2876909/1‑148 (MQ=255)
                                                                                                   tCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTg                                           >  1:1819656/1‑148 (MQ=255)
                                                                                                   tCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTg                                           >  2:469928/1‑148 (MQ=255)
                                                                                                   tCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTg                                           >  2:3131304/1‑148 (MQ=255)
                                                                                                    aaCCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGAAGTTCAGCATCAAGAGCTGTTTTCTTCACTTGATCGCGGAACTgg                                          >  1:1963043/2‑148 (MQ=255)
                                                                                                      ccGCTAAATTAAGTGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGt                                        <  1:2821078/148‑1 (MQ=255)
                                                                                                      ccGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGt                                        >  2:1895582/1‑148 (MQ=255)
                                                                                                       cGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGtt                                       <  1:1356214/148‑1 (MQ=255)
                                                                                                          tAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAAc                                    >  1:259106/1‑148 (MQ=255)
                                                                                                            aaTTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAg                                  <  2:27730/148‑1 (MQ=255)
                                                                                                              ttAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCg                                <  2:3147049/148‑1 (MQ=255)
                                                                                                              ttAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCg                                >  2:3371/1‑148 (MQ=255)
                                                                                                                   ctgATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTc                           <  2:2144381/146‑1 (MQ=255)
                                                                                                                    ggATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCg                          <  1:1010259/148‑1 (MQ=255)
                                                                                                                      aTTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGa                        <  1:1087995/148‑1 (MQ=255)
                                                                                                                      aTTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACGGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGa                        <  2:1712368/148‑1 (MQ=255)
                                                                                                                       ttCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGAt                       <  2:1187321/148‑1 (MQ=255)
                                                                                                                        tCAAGTACCACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATc                      <  2:163616/148‑1 (MQ=255)
                                                                                                                        tCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATc                      <  2:1771365/148‑1 (MQ=255)
                                                                                                                           aGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAg                   <  1:3528302/148‑1 (MQ=255)
                                                                                                                            gTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGc                  <  1:2111361/148‑1 (MQ=255)
                                                                                                                            gTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGc                  >  1:2232993/1‑148 (MQ=255)
                                                                                                                              accacaGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTg                <  2:2619437/146‑1 (MQ=255)
                                                                                                                                 acaGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTAc             <  1:2780313/148‑1 (MQ=255)
                                                                                                                                 acaGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTAc             <  2:1484963/148‑1 (MQ=255)
                                                                                                                                  caGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACa            <  2:2880931/148‑1 (MQ=255)
                                                                                                                                  caGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACa            <  1:2151171/148‑1 (MQ=255)
                                                                                                                                    ggACTTACTCATATTCTAGATTAGTATAACAGTACTAACCTGCTTCGAACTCTTCATGGAAGTGTTGTTGCAGTTCAGCATCTCGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTTACAGGGTTTTCGTATCGAGCTGttgata          <  1:2348014/148‑6 (MQ=255)
                                                                                                                                    ggACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACata          <  2:1897883/148‑1 (MQ=255)
                                                                                                                                     gACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACatat         <  1:439941/148‑1 (MQ=255)
                                                                                                                                      aCTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATAtt        >  2:490823/1‑148 (MQ=255)
                                                                                                                                        ttACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTgc      >  2:3489616/1‑148 (MQ=255)
                                                                                                                                        ttACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGGTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTgc      <  2:2432610/148‑1 (MQ=255)
                                                                                                                                          aCTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTgcag    <  2:910845/148‑1 (MQ=255)
                                                                                                                                           cTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTgcagc   >  1:1826251/1‑148 (MQ=255)
                                                                                                                                            tCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTgcagca  <  1:2626553/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GATGGACTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCA  >  CP009273/3077158‑3077445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGGACTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCG  >  CP009273/3077159‑3077451
                                                                                                                                               |                                                                                                                                                     
ATGGACTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAG                                                                                                                                                 >  SRR19973066.3378547/1‑150 (MQ=60)
 TGGACTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGA                                                                                                                                                >  SRR19973066.28121/1‑150 (MQ=60)
  GGACTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGAT                                                                                                                                               <  SRR19973066.1749007/150‑1 (MQ=60)
   GACTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATA                                                                                                                                              >  SRR19973066.792072/1‑150 (MQ=60)
      TCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGT                                                                                                                                           <  SRR19973066.2650516/150‑1 (MQ=60)
         TATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATA                                                                                                                                        <  SRR19973066.3203403/150‑1 (MQ=60)
            TACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGAATTACTCATATTCAAGATAAGTATAACC                                                                                                                                     <  SRR19973066.768407/150‑1 (MQ=60)
             ACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCG                                                                                                                                    <  SRR19973066.2241241/150‑1 (MQ=60)
              CAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGT                                                                                                                                   >  SRR19973066.917507/1‑150 (MQ=60)
              CAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGT                                                                                                                                   >  SRR19973066.917311/1‑150 (MQ=60)
              CAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGT                                                                                                                                   >  SRR19973066.898586/1‑150 (MQ=60)
                 AGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACA                                                                                                                                >  SRR19973066.2900225/1‑150 (MQ=60)
                 AGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACA                                                                                                                                <  SRR19973066.1163329/150‑1 (MQ=60)
                  GTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAA                                                                                                                               >  SRR19973066.1776529/1‑150 (MQ=60)
                    CGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAAC                                                                                                                             <  SRR19973066.2410518/150‑1 (MQ=60)
                    CGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAAC                                                                                                                             >  SRR19973066.1911422/1‑150 (MQ=60)
                     GATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACC                                                                                                                            <  SRR19973066.2562561/150‑1 (MQ=60)
                      ATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCT                                                                                                                           >  SRR19973066.3533673/1‑150 (MQ=60)
                      ATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCT                                                                                                                           >  SRR19973066.3533687/1‑150 (MQ=60)
                           AAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTC                                                                                                                      >  SRR19973066.1972528/1‑150 (MQ=60)
                           AAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTC                                                                                                                      >  SRR19973066.610601/1‑150 (MQ=60)
                            AAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCG                                                                                                                     >  SRR19973066.2693613/1‑150 (MQ=60)
                             AAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGTTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGA                                                                                                                    >  SRR19973066.2512229/1‑150 (MQ=60)
                             AAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGTTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGA                                                                                                                    >  SRR19973066.2902051/1‑150 (MQ=60)
                              AAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAA                                                                                                                   >  SRR19973066.913229/1‑150 (MQ=60)
                              AAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAA                                                                                                                   >  SRR19973066.2774031/1‑150 (MQ=60)
                               AACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAAC                                                                                                                  >  SRR19973066.2257703/1‑150 (MQ=60)
                                  CCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCT                                                                                                               <  SRR19973066.3035501/150‑1 (MQ=60)
                                  CCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCT                                                                                                               >  SRR19973066.3579066/1‑150 (MQ=60)
                                  CCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTAAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCT                                                                                                               >  SRR19973066.3578903/1‑150 (MQ=60)
                                  CCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCACTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCT                                                                                                               >  SRR19973066.2298531/1‑150 (MQ=60)
                                    TTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTC                                                                                                             <  SRR19973066.1447491/150‑1 (MQ=60)
                                    TTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTC                                                                                                             >  SRR19973066.772948/1‑150 (MQ=60)
                                      CCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAA                                                                                                           <  SRR19973066.2442772/150‑1 (MQ=60)
                                       CGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAG                                                                                                          <  SRR19973066.2298821/150‑1 (MQ=60)
                                       AGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAG                                                                                                          <  SRR19973066.3510350/150‑1 (MQ=60)
                                       AGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAG                                                                                                          <  SRR19973066.2018862/150‑1 (MQ=60)
                                          GTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAA                                                                                                       <  SRR19973066.354717/150‑1 (MQ=60)
                                            CGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACT                                                                                                     >  SRR19973066.2983918/1‑150 (MQ=60)
                                            CGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACT                                                                                                     >  SRR19973066.1195652/1‑150 (MQ=60)
                                             GTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTG                                                                                                    >  SRR19973066.884648/1‑150 (MQ=60)
                                              TTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGT                                                                                                   >  SRR19973066.3400577/1‑150 (MQ=60)
                                              TTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGT                                                                                                   <  SRR19973066.2151891/150‑1 (MQ=60)
                                                      GACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAG                                                                                           >  SRR19973066.559333/1‑150 (MQ=60)
                                                        CGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTT                                                                                         <  SRR19973066.3547432/150‑1 (MQ=60)
                                                             AAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCA                                                                                    <  SRR19973066.174130/150‑1 (MQ=60)
                                                                GGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCA                                                                                 >  SRR19973066.3494016/1‑150 (MQ=60)
                                                                   TGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGAAGTTCAGCATCAAGA                                                                              >  SRR19973066.3168036/1‑150 (MQ=60)
                                                                   TGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAAATGTTGTTGCAGTTCAGCATCAAGA                                                                              >  SRR19973066.3168219/1‑150 (MQ=60)
                                                                    GGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGAT                                                                             >  SRR19973066.1783001/1‑150 (MQ=60)
                                                                         TGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTT                                                                        >  SRR19973066.1191382/1‑150 (MQ=60)
                                                                          GTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTT                                                                       <  SRR19973066.1887841/150‑1 (MQ=60)
                                                                          GTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTT                                                                       <  SRR19973066.1887922/150‑1 (MQ=60)
                                                                              CAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTT                                                                   <  SRR19973066.2741826/150‑1 (MQ=60)
                                                                               AATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTC                                                                  >  SRR19973066.1708704/1‑150 (MQ=60)
                                                                                ATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCA                                                                 >  SRR19973066.535267/1‑150 (MQ=60)
                                                                                ATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCA                                                                 <  SRR19973066.3330631/150‑1 (MQ=60)
                                                                                   AATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTT                                                              >  SRR19973066.2534311/1‑150 (MQ=60)
                                                                                       GTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATC                                                          <  SRR19973066.420514/150‑1 (MQ=60)
                                                                                       GTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATC                                                          <  SRR19973066.420299/150‑1 (MQ=60)
                                                                                         GGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGC                                                        >  SRR19973066.1786735/1‑150 (MQ=60)
                                                                                          GCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCG                                                       <  SRR19973066.3446098/150‑1 (MQ=60)
                                                                                           CGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGG                                                      >  SRR19973066.1522736/1‑150 (MQ=60)
                                                                                              ATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAAC                                                   >  SRR19973066.1150259/1‑150 (MQ=60)
                                                                                               TTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACT                                                  <  SRR19973066.3191877/150‑1 (MQ=60)
                                                                                                TATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTG                                                 >  SRR19973066.1832624/1‑150 (MQ=60)
                                                                                                 ATAACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGAAGTTCAGCATCAAGAGCTGTTTTCTTCACTTGATCGCGGAACTGG                                                >  SRR19973066.1976921/1‑150 (MQ=60)
                                                                                                   CACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGT                                              >  SRR19973066.1067341/1‑150 (MQ=60)
                                                                                                   CACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGT                                              >  SRR19973066.2148226/1‑150 (MQ=60)
                                                                                                     CCGCTAAATTAAGTGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTA                                            <  SRR19973066.2840036/150‑1 (MQ=60)
                                                                                                     CCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTA                                            <  SRR19973066.480867/150‑1 (MQ=60)
                                                                                                      CGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAA                                           <  SRR19973066.1366039/150‑1 (MQ=60)
                                                                                                      CGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAA                                           <  SRR19973066.1366034/150‑1 (MQ=60)
                                                                                                       GCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAAC                                          >  SRR19973066.261060/1‑150 (MQ=60)
                                                                                                       GCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAAC                                          >  SRR19973066.260232/1‑150 (MQ=60)
                                                                                                                   GGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGA                              <  SRR19973066.1017704/150‑1 (MQ=60)
                                                                                                                   GGATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGA                              >  SRR19973066.2481620/1‑150 (MQ=60)
                                                                                                                     ATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATC                            <  SRR19973066.1095930/150‑1 (MQ=60)
                                                                                                                     ATTCAAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATC                            <  SRR19973066.735329/150‑1 (MQ=60)
                                                                                                                         AAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGC                        >  SRR19973066.2248405/1‑150 (MQ=60)
                                                                                                                         AAGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGC                        <  SRR19973066.1839148/150‑1 (MQ=60)
                                                                                                                          AGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCT                       <  SRR19973066.3551772/150‑1 (MQ=60)
                                                                                                                          AGTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCT                       <  SRR19973066.39550/150‑1 (MQ=60)
                                                                                                                           GTAACACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTG                      <  SRR19973066.2125944/150‑1 (MQ=60)
                                                                                                                                ACAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACAT                 <  SRR19973066.2799030/150‑1 (MQ=60)
                                                                                                                                 CAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATA                <  SRR19973066.2166104/150‑1 (MQ=60)
                                                                                                                                 CAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATA                <  SRR19973066.2611319/150‑1 (MQ=60)
                                                                                                                                 CAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATA                <  SRR19973066.12405/150‑1 (MQ=60)
                                                                                                                                 CAGGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATA                <  SRR19973066.2165000/150‑1 (MQ=60)
                                                                                                                                   GGACTTACTCATATTCTAGATTAGTATAACAGTACTAACCTGCTTCGAACTCTTCATGGAAGTGTTGTTGCAGTTCAGCATCTCGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTTACAGGGTTTTCGTATCGAGCTGTTGATATT              <  SRR19973066.2363979/150‑1 (MQ=60)
                                                                                                                                   GGACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATT              >  SRR19973066.185228/1‑150 (MQ=60)
                                                                                                                                    GACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTG             <  SRR19973066.443343/150‑1 (MQ=60)
                                                                                                                                    GACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTG             >  SRR19973066.864326/1‑150 (MQ=60)
                                                                                                                                    GACTTACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTG             <  SRR19973066.2804033/150‑1 (MQ=60)
                                                                                                                                        TACTCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGC         >  SRR19973066.1839280/1‑150 (MQ=60)
                                                                                                                                           TCATATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATG      <  SRR19973066.2644260/150‑1 (MQ=60)
                                                                                                                                               ATTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCG  >  SRR19973066.2705497/1‑150 (MQ=60)
                                                                                                                                               |                                                                                                                                                     
ATGGACTCCTATTACAAAGTCGATATAAAAAAACCCTTCCGCGTCGTTAGGCCCGACGAGGAAGGGTTGGATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCG  >  CP009273/3077159‑3077451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑