Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F368 I0 R1	1472	214.4	7116750	95.9%	6824963	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,262,740	T→A	38.7%	Y323N (TAT→AAT)	yhaC →	pentapetide repeats‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,262,740	0	T	A	38.7%	225.4 / inf	266	Y323N (TAT→AAT)	yhaC	pentapetide repeats‑related protein
Reads supporting (aligned to +/- strand): ref base T (84/79); new base A (51/52); total (135/131)
Fisher's exact test for biased strand distribution p-value = 8.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 269 total aligned reads displayed.

ACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAG  >  CP009273/3262593‑3262887

   aaaGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATc                                                                                                                                                  <  2:1231964/148‑1 (MQ=255)

   aaaGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATc                                                                                                                                                  >  2:1348426/1‑148 (MQ=255)

       aaaTATATAAAAGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGgt                                                                                                                                              <  1:3185581/148‑1 (MQ=255)

       aaaTATATAAAAGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGgt                                                                                                                                              >  2:2874116/1‑148 (MQ=255)

       aaaTATATAAAAGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGATAATATGgt                                                                                                                                              >  2:1542283/1‑148 (MQ=255)

              taAAAGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTcccc                                                                                                                                       >  1:3001563/1‑148 (MQ=255)

                aaaGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCa                                                                                                                                     >  1:3362893/1‑148 (MQ=255)

                 aaGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCaa                                                                                                                                    >  1:2041053/1‑148 (MQ=255)

                  aGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCaaa                                                                                                                                   >  2:563166/1‑148 (MQ=255)

                  aGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCaaa                                                                                                                                   >  1:2471154/1‑148 (MQ=255)

                   gCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAaat                                                                                                                                  >  2:381503/1‑148 (MQ=255)

                     tCATCTAAAATCATCAATTGCTTGAACAGAATTAAATTAACCGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAaatta                                                                                                                                <  1:2783385/148‑1 (MQ=255)

                      cATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAaattaa                                                                                                                               <  1:1952896/148‑1 (MQ=255)

                      cATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAaattaa                                                                                                                               >  1:293163/1‑148 (MQ=255)

                      cATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAaattaa                                                                                                                               >  1:916610/1‑148 (MQ=255)

                      cATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAaattaa                                                                                                                               <  1:2277875/148‑1 (MQ=255)

                      cATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAaattaa                                                                                                                               <  2:1549923/148‑1 (MQ=255)

                       aTCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAaattaat                                                                                                                              >  1:3375673/1‑148 (MQ=255)

                        tCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAaattaatt                                                                                                                             <  1:1170417/148‑1 (MQ=255)

                         cGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAattaatta                                                                                                                            >  2:45655/1‑148 (MQ=255)

                          gAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATtat                                                                                                                           <  2:2572006/148‑1 (MQ=255)

                              atcatcAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTa                                                                                                                       >  1:2217563/1‑148 (MQ=255)

                                catcaATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATg                                                                                                                     >  2:2490964/1‑148 (MQ=255)

                                  tcaATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGa                                                                                                                   >  2:1791005/1‑148 (MQ=255)

                                   caATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGAt                                                                                                                  >  2:1396895/1‑148 (MQ=255)

                                   caATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGAt                                                                                                                  <  1:1009871/148‑1 (MQ=255)

                                       tGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTaa                                                                                                              <  2:2235018/148‑1 (MQ=255)

                                        gCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTaaa                                                                                                             <  2:607356/148‑1 (MQ=255)

                                              acacAATTAAATTAACAGAGATGAAAGAAATCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATaga                                                                                                       <  2:2125700/148‑1 (MQ=255)

                                              aCAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATaga                                                                                                       >  1:3271762/1‑148 (MQ=255)

                                                aGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATagaag                                                                                                     >  2:3001525/1‑148 (MQ=255)

                                                 taattaaattaACAGAGTTGTTTGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATagaaga                                                                                                    <  2:3245651/147‑1 (MQ=255)

                                                 gAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAAAATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATagaaga                                                                                                    >  2:1641943/1‑148 (MQ=255)

                                                  aattaaattaACAGAGATTAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCACAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGAc                                                                                                   <  2:1854326/148‑1 (MQ=255)

                                                   attaaattaaCAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGAct                                                                                                  >  2:740354/1‑148 (MQ=255)

                                                   attaaattaaCAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGAct                                                                                                  >  1:1937390/1‑148 (MQ=255)

                                                     taaattaaCAGAGATGAAAGAATTCTCATCATAAAAAATATATGATTACATCTATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGActct                                                                                                <  2:3315239/148‑1 (MQ=255)

                                                      aaattaaCAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCTCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTa                                                                                               >  2:1471996/1‑148 (MQ=255)

                                                       aattaaCAGATATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAACAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAg                                                                                              <  1:49648/148‑1 (MQ=255)

                                                         ttaaCAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCt                                                                                            <  2:702336/148‑1 (MQ=255)

                                                         ttaaCAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCt                                                                                            >  1:2600746/1‑148 (MQ=255)

                                                               gagaTGAAAGAATTCTCATCAGAAAAAATATATTATTACATCGATATAATCATTGAAGATTATGAAAATACCAAACAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCagaag                                                                                      <  1:2128938/148‑1 (MQ=255)

                                                               gagaTGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCagaag                                                                                      >  2:3132703/1‑148 (MQ=255)

                                                               gagaTGAAACAATTCTCATCATAAAAAATATATGATTACATCTATATAATAATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCagaag                                                                                      <  1:339185/148‑1 (MQ=255)

                                                                    gAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTa                                                                                 <  2:3335347/148‑1 (MQ=255)

                                                                     aaaGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTAc                                                                                <  1:285033/148‑1 (MQ=255)

                                                                       aGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACtt                                                                              >  2:635680/1‑148 (MQ=255)

                                                                        gAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACttt                                                                             >  1:3556901/1‑148 (MQ=255)

                                                                        gAATTCTCAACAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACttt                                                                             <  1:3025003/148‑1 (MQ=255)

                                                                        caagcctcATAACAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACttt                                                                             <  1:2524370/143‑1 (MQ=255)

                                                                          aTTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCa                                                                           <  1:2966714/148‑1 (MQ=255)

                                                                           ttCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAg                                                                          <  2:1003782/148‑1 (MQ=255)

                                                                              tcatcaGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGata                                                                       >  2:1123702/1‑148 (MQ=255)

                                                                                atcaGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGataat                                                                     <  1:594497/148‑1 (MQ=255)

                                                                                 tcaGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGataata                                                                    <  1:3540132/148‑1 (MQ=255)

                                                                                 tcaGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGataata                                                                    >  2:180541/1‑148 (MQ=255)

                                                                                  caGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATAc                                                                   <  1:476235/148‑1 (MQ=255)

                                                                                    gAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAACGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATAccc                                                                 <  1:3091820/148‑1 (MQ=255)

                                                                                    gAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATAccc                                                                 >  2:2436814/1‑148 (MQ=255)

                                                                                    gAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCAAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATAccc                                                                 <  1:1190309/148‑1 (MQ=255)

                                                                                      aaaaaTATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTc                                                               >  2:1046946/1‑148 (MQ=255)

                                                                                           tatatGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGaa                                                          >  2:2791192/1‑148 (MQ=255)

                                                                                             tatGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAg                                                        <  2:2909909/148‑1 (MQ=255)

                                                                                              atGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGa                                                       >  2:2807825/1‑148 (MQ=255)

                                                                                              atGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGa                                                       <  2:2894648/148‑1 (MQ=255)

                                                                                               tGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGAc                                                      >  2:2370931/1‑148 (MQ=255)

                                                                                                 aTTACATCTATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGa                                                    <  2:2541009/148‑1 (MQ=255)

                                                                                                      aTCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAAtt                                               >  2:930415/1‑148 (MQ=255)

                                                                                                       tCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATtc                                              <  2:2318251/148‑1 (MQ=255)

                                                                                                          atatAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTcc                                           >  2:648475/1‑148 (MQ=255)

                                                                                                            ataATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCgg                                         <  2:371508/148‑1 (MQ=255)

                                                                                                               aTCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACa                                      >  1:1004130/1‑148 (MQ=255)

                                                                                                                tCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACaa                                     >  2:304996/1‑148 (MQ=255)

                                                                                                                tCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACaa                                     >  2:870948/1‑148 (MQ=255)

                                                                                                                tCATTGAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACaa                                     >  1:1376625/1‑148 (MQ=255)

                                                                                                                   ttGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATgg                                  <  2:54682/148‑1 (MQ=255)

                                                                                                                    tGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGc                                 >  1:1838560/1‑148 (MQ=255)

                                                                                                                    tGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGc                                 >  1:1243087/1‑148 (MQ=255)

                                                                                                                    tGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGc                                 <  2:3348752/148‑1 (MQ=255)

                                                                                                                     gAAGATTATGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCt                                <  2:167405/148‑1 (MQ=255)

                                                                                                                      aatattattAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCtt                               <  1:1622461/145‑1 (MQ=255)

                                                                                                                        gATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTg                             <  1:1928898/148‑1 (MQ=255)

                                                                                                                            aTGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGt                         <  2:1941039/148‑1 (MQ=255)

                                                                                                                             tGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTc                        >  2:2016881/1‑148 (MQ=255)

                                                                                                                             tGAAAATACCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGGCTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTc                        >  2:2306829/1‑148 (MQ=255)

                                                                                                                               aaaaTACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGTATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCgg                      <  1:990303/148‑1 (MQ=255)

                                                                                                                               aaaaTACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCgg                      >  1:655570/1‑148 (MQ=255)

                                                                                                                               aaaaTACCAAAGAAAATGCGTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCgg                      <  1:3485809/148‑1 (MQ=255)

                                                                                                                                aaaTACCAAACAAAATGCTTATCTGGTCGTCCCCAAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCggg                     <  1:67109/148‑1 (MQ=255)

                                                                                                                                  aTACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTATCCGGACAATGGCTTTGAGGTCGGGGa                   >  2:67163/1‑148 (MQ=255)

                                                                                                                                   tACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGaa                  <  1:563166/148‑1 (MQ=255)

                                                                                                                                    aCCAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAat                 >  1:758200/1‑148 (MQ=255)

                                                                                                                                     ccAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAata                >  1:2737060/1‑148 (MQ=255)

                                                                                                                                     ccAAAGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAata                <  1:224713/148‑1 (MQ=255)

                                                                                                                                        aaGAAAATGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATaa             <  2:2665666/148‑1 (MQ=255)

                                                                                                                                           aaaaTGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGCCGAAAATTCTCCGGACACTGGCTTGGAGGTCGGGGCCTATAccac          >  2:3294782/1‑148 (MQ=255)

                                                                                                                                           aaaaTGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAacac          <  2:3375673/148‑1 (MQ=255)

                                                                                                                                             aaTGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACat        <  1:727989/148‑1 (MQ=255)

                                                                                                                                             aaTGCTAATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACat        <  1:727386/148‑1 (MQ=255)

                                                                                                                                              aTGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACata       <  2:1164850/148‑1 (MQ=255)

ACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAG  >  CP009273/3262593‑3262887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A