Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,263,409 C→T 100% intergenic (+448/+166) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,263,4090CT85.0% 411.7 / 65.0 194intergenic (+448/+166)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base C (17/11);  new base T (63/99);  total (83/111)
Fisher's exact test for biased strand distribution p-value = 3.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 194 total aligned reads displayed.

CTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGA  >  CP009273/3263263‑3263554
                                                                                                                                                  |                                                                                                                                                 
         ataGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCt                                                                                                                                        <  2:1523912/149‑1 (MQ=37)
            gtgtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCa                                                                                                                                     <  2:839287/149‑1 (MQ=37)
               tCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCaaaa                                                                                                                                  <  1:941083/149‑1 (MQ=37)
                 gTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAAAGTCATTCATTTTTGTGGCGCTCACAAAGc                                                                                                                                >  2:3198325/1‑149 (MQ=11)
                 gTGGAAAGTCATTCATATTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGc                                                                                                                                <  1:4425190/149‑1 (MQ=255)
                    gACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCaaa                                                                                                                             >  1:323106/1‑149 (MQ=37)
                    gACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCaaa                                                                                                                             <  1:3014641/149‑1 (MQ=37)
                      cagtcagtCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCaaaaa                                                                                                                           <  2:2983144/149‑1 (MQ=255)
                        gTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTATTCTGCCCCTCCAAAAGCAAAAAcc                                                                                                                         >  2:2749229/1‑149 (MQ=255)
                         tcattcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAccc                                                                                                                        >  1:4308941/1‑149 (MQ=37)
                          cattcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACccg                                                                                                                       <  1:2958324/149‑1 (MQ=37)
                           attcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTATTCCCCGCCAAAAGCAAAAACccgc                                                                                                                      >  1:3761874/1‑149 (MQ=255)
                              catCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGa                                                                                                                   >  2:2219027/1‑149 (MQ=21)
                              catCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTCTAGAGTCGTGGACAGTCCTTCATCTTTCTGCCCCGCCAAAAGCACAAACCCGCCGa                                                                                                                   >  2:2377949/1‑149 (MQ=11)
                               taatttCTGCCACTCAAAAATTAAAAACCCGCCGTAGCGTTTTTTTACGTAAAACATGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCAAAAAGCAAAAACCCGCCGaa                                                                                                                  <  1:418855/146‑1 (MQ=11)
                               atCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGaa                                                                                                                  <  1:128304/149‑1 (MQ=37)
                               atCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGaa                                                                                                                  >  2:964418/1‑149 (MQ=37)
                                   ttCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                                              <  1:154602/149‑1 (MQ=37)
                                   ttCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTAATTGATTTTTCTTGCCCTCCAAAAGCAAAATACCGACGACGCgg                                                                                                              >  2:4513188/1‑149 (MQ=11)
                                   ttCGGCCCCTCCACACTTAAAAACCCGCCGAAGCGGTTTTTTACGTAAAACAGTTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAACAGCAAAAACCCGCCGAAGCgg                                                                                                              <  2:2266205/149‑1 (MQ=11)
                                       gCCCCTCAAAAATAAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                          <  2:527659/149‑1 (MQ=11)
                                        ccccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATATTGTCGTGGACCGGCATTGATTTTTATGCCCCTCCCAAAGCAATAACCCGCCGAAGCGGGtttt                                                                                                         >  1:2050501/1‑149 (MQ=11)
                                         cccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                                        <  1:2677728/149‑1 (MQ=37)
                                         cccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGGAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGTGTCGTGGACAGTCATTCCTGTTGCGGCCCCTCCAAAAGCAAAAACCCGCCGCAGCgggggttt                                                                                                        >  2:3694434/1‑144 (MQ=11)
                                          ccTCCACAAGTAAACACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                       <  2:3721962/149‑1 (MQ=255)
                                                aaaGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGTCAGTCAATCAATTTTATTCCCCTCCAAAAGAAAAAAACCGCCGAAGCGGGTTTTTAAGTaaa                                                                                                 >  1:1645561/1‑149 (MQ=11)
                                                  aGTAAAAACCCGCCGCAGCGGTTTTTTACGTACAACAGGTGAAACTGACCGAGAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAcc                                                                                               <  2:1618201/149‑1 (MQ=11)
                                                     aaaaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAgg                                                                                            <  1:4015061/149‑1 (MQ=255)
                                                      aaatCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGt                                                                                           <  2:3621770/149‑1 (MQ=37)
                                                      aaacCCCGCCCTAGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGt                                                                                           <  2:1982524/149‑1 (MQ=255)
                                                      aaaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt                                                                                           >  1:65825/1‑149 (MQ=17)
                                                       aaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTg                                                                                          <  2:4285980/149‑1 (MQ=37)
                                                          cccGCCGCACCGGGTTCTGACGTACAACCGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaaa                                                                                       <  2:4032822/149‑1 (MQ=11)
                                                          cccGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaaa                                                                                       <  2:4385346/149‑1 (MQ=37)
                                                          cccGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaaa                                                                                       <  1:2794522/149‑1 (MQ=37)
                                                             gccgAAGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTg                                                                                    <  2:2508220/149‑1 (MQ=255)
                                                             gccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTg                                                                                    <  2:4121260/149‑1 (MQ=37)
                                                              ccgCAGCGGGTTTTTACGTACAACCGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa                                                                                   <  2:1800899/149‑1 (MQ=255)
                                                                gAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc                                                                                 <  1:2093959/149‑1 (MQ=37)
                                                                 ttgCGGTTTTTTACGTATATCTGGTTAATGTGACCGATTAGCCTCTTTCTTTTGTGTATAGTGTCGTGGACAGTAATTCATTTTTCTGCCCCTCCAAAATAAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCg                                                                                <  1:2433330/147‑1 (MQ=11)
                                                                  aGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAACTTTCTGCCCCTCCAAAAGCAAAAAACCGCCCAAGCGGGTTTTTACGTAAAACAGGTGAAACAGACCGa                                                                               >  1:3319373/1‑149 (MQ=11)
                                                                   gCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGAt                                                                              <  2:920678/149‑1 (MQ=255)
                                                                    cGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATa                                                                             <  1:1999150/149‑1 (MQ=255)
                                                                    cGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATa                                                                             <  1:2192136/149‑1 (MQ=37)
                                                                       gTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGc                                                                          <  2:1845381/149‑1 (MQ=37)
                                                                        tttttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGcc                                                                         <  1:3310552/149‑1 (MQ=37)
                                                                        tttttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCCCCGAAGCGGGTTTTTACGTAAACCCGGTGAAACTGACCGAAAAGcc                                                                         >  2:3332057/1‑149 (MQ=255)
                                                                         ttttACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCCTTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCg                                                                        <  2:2618663/149‑1 (MQ=255)
                                                                         ttttACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCg                                                                        <  2:2618689/149‑1 (MQ=255)
                                                                          tttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGCAGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAAACCCCCGCAGCCGGTTTTTACGTAAACCAGGTTAAAATGACCGATAAACCGc                                                                       >  1:3599219/1‑149 (MQ=11)
                                                                          tttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGc                                                                       <  1:4184132/149‑1 (MQ=38)
                                                                          tttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCTCTCCAAAAGCAAAAACCCGCCGACGCGGGGTTTTACGTAAACCAGGTGAAACTGACCGAAAAGCCGc                                                                       >  2:4080018/1‑149 (MQ=255)
                                                                            tACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCGGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctt                                                                     <  2:242676/149‑1 (MQ=255)
                                                                             aCGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGcttt                                                                    <  2:1250159/149‑1 (MQ=255)
                                                                              cGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttc                                                                   <  2:4329333/149‑1 (MQ=255)
                                                                               gTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttct                                                                  <  1:2351213/149‑1 (MQ=255)
                                                                                 acaaCAGGTCAAACTGCCCGATACGCCGCTTTCTTTTGGGTAGAGTGTCGTGGACTGTCCTTCAATTTTCTGCCCCTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttcttt                                                                <  2:555190/147‑1 (MQ=11)
                                                                                 aaaaCCGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttcttt                                                                <  1:3831615/149‑1 (MQ=255)
                                                                                 aaaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttcttt                                                                >  2:339294/1‑149 (MQ=255)
                                                                                  aaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                               <  1:1319675/149‑1 (MQ=255)
                                                                                  aaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTATGCCCCTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTTAGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTAtttt                                                               >  1:72589/1‑149 (MQ=14)
                                                                                  aaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                               >  1:771760/1‑149 (MQ=255)
                                                                                   atcAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                              <  2:4409917/147‑1 (MQ=2)
                                                                                   aaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                              <  1:4580239/149‑1 (MQ=38)
                                                                                   aaCAGGTGAAACTGACAGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGGTTTCTTTTg                                                              >  2:2290499/1‑149 (MQ=255)
                                                                                     cAGGTGAAACGGACCGATAAGCCGCTTTCTTTTGGGTCTAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTggg                                                            <  2:1106535/149‑1 (MQ=255)
                                                                                      aGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGt                                                           <  2:4674268/149‑1 (MQ=21)
                                                                                      aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTgggg                                                           >  2:1819699/1‑148 (MQ=255)
                                                                                          gAAACTGACCGATAAGCCGCTTTCTTTTGGGTATCGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAg                                                       <  1:3828983/149‑1 (MQ=25)
                                                                                          gAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAg                                                       <  1:223649/149‑1 (MQ=25)
                                                                                          gAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAg                                                       <  2:2314779/149‑1 (MQ=25)
                                                                                          gAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAg                                                       <  1:223653/149‑1 (MQ=25)
                                                                                            aaCTGACCGATAAGCCGCTTTCTTTTGGGTCTAGTGTCGACGAAAGACTATCTAATTTCTGCCACTCCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcg                                                     <  2:678371/149‑1 (MQ=11)
                                                                                              cTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGGAAAGcgtc                                                   >  2:3168058/1‑149 (MQ=255)
                                                                                              cTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtc                                                   >  2:4540432/1‑149 (MQ=255)
                                                                                               tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcg                                                  >  2:1840239/1‑149 (MQ=37)
                                                                                                gACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgt                                                 <  1:20918/149‑1 (MQ=37)
                                                                                                    gATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGCGTCGAGGAc                                             >  2:4348523/1‑149 (MQ=255)
                                                                                                     aTACGCCGCGTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCACTGTTCTGCCCCTCCAAAAGCAAAAACCCGCCGTAGCGGGTTTTTACGTACACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACa                                            <  2:4206496/149‑1 (MQ=11)
                                                                                                     aTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGCGTCGTGGACa                                            >  2:1375874/1‑149 (MQ=255)
                                                                                                     aTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAATGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGaga                                            >  2:4776566/1‑147 (MQ=19)
                                                                                                       aaGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAATTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTAGGGTATAGCGTATTGGACAGt                                          >  1:1121860/1‑149 (MQ=11)
                                                                                                          ccGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAAATTTTATGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAACGATAATCCGCTTTCTTTTGGGTATAGCGTCGTGGTCAGtcca                                       >  1:3504524/1‑147 (MQ=11)
                                                                                                          ccGCTGTCTTTTGGGTCTCGTGTCGTGGACAGTCATTCCTTGTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGTTTTTACGTACACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcat                                       <  2:3980035/149‑1 (MQ=11)
                                                                                                            gCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACAGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtccatc                                     >  1:2073713/1‑145 (MQ=255)
                                                                                                             ctttctttTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGCCAggccatca                                    >  2:871860/1‑142 (MQ=11)
                                                                                                               ttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATc                                  <  1:3914190/149‑1 (MQ=37)
                                                                                                                  ttttTGGAATACGGTCTTGGTAAGTCAGTCCTTTTTCGGGCCCTCCACAATCTAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCgtt                               <  2:1227594/149‑1 (MQ=11)
                                                                                                                             gtgtCGTGGACAGTCCTTCATTTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAGTAATATTTCAGCCccacca                    >  1:1097910/1‑149 (MQ=11)
                                                                                                                              tgtCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaa                   <  1:1767539/149‑1 (MQ=37)
                                                                                                                              tgtCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCaa                   >  1:2697399/1‑149 (MQ=255)
                                                                                                                                  gTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCTTGGACAGTCATTCATCTTTCTGCACCTCCAAAAGc               >  2:3052251/1‑149 (MQ=255)
                                                                                                                                  gTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaatc               >  1:772227/1‑147 (MQ=37)
                                                                                                                                    ggACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCaa             >  1:103071/1‑149 (MQ=255)
                                                                                                                                     gACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCGTTCTGCCCCTCCAAAAGCaaa            >  1:2615989/1‑149 (MQ=37)
                                                                                                                                      aCAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAATCaaaa           >  1:4720891/1‑149 (MQ=255)
                                                                                                                                        aGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGTATAGCGTCGTGGAAAGTCATTCATAATTAAGCCCCCACAAAAGCAAAAAc         >  1:4717376/1‑149 (MQ=11)
                                                                                                                                         gTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAcc        >  1:1044942/1‑149 (MQ=37)
                                                                                                                                            tttcatTTTTCTGCCCCTCAAAAAGGTAAAACCAGCCGACGCGGGTTTTTACGTTAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgc     <  1:1511538/148‑1 (MQ=11)
                                                                                                                                              tcatTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACCGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgccg   >  2:2657858/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGA  >  CP009273/3263263‑3263554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG  >  CP009273/3263261‑3263558
                                                                                                                                                    |                                                                                                                                                     
TTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTT                                                                                                                                                      <  SRR19973071.991301/150‑1 (MQ=60)
 TCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCACAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT                                                                                                                                                     <  SRR19973071.3273517/150‑1 (MQ=31)
 TCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT                                                                                                                                                     <  SRR19973071.1636398/150‑1 (MQ=60)
  CTTTTGGGTATCGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTT                                                                                                                                                    <  SRR19973071.300888/150‑1 (MQ=32)
  CTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTT                                                                                                                                                    <  SRR19973071.2702405/150‑1 (MQ=32)
             AGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCA                                                                                                                                         <  SRR19973071.4377500/150‑1 (MQ=32)
                 TCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAG                                                                                                                                     <  SRR19973071.944504/150‑1 (MQ=32)
                  CGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGC                                                                                                                                    <  SRR19973071.2080497/150‑1 (MQ=32)
                  CGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATTTTTCTGCCCCgcccaaatc                                                                                                                                    >  SRR19973071.471140/1‑141 (MQ=31)
                   GTGGAAAGTCATTCATATTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCA                                                                                                                                   <  SRR19973071.4440798/150‑1 (MQ=31)
                    TGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATTTTTATGCCCCTCCAAAAGCAA                                                                                                                                  >  SRR19973071.1050065/1‑150 (MQ=30)
                     GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTATGCCCCTCCAAAAGCAAA                                                                                                                                 >  SRR19973071.324209/1‑150 (MQ=31)
                     GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTTCGCCTCCAAAAGCAAA                                                                                                                                 >  SRR19973071.324239/1‑150 (MQ=60)
                      GACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAA                                                                                                                                <  SRR19973071.3025395/150‑1 (MQ=32)
                      GACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAAAGTCATTCAATATTCAGCCCCACCAAAAGCAAAA                                                                                                                                >  SRR19973071.1569587/1‑150 (MQ=27)
                       ACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGTGTCGTGGCAAGTCATTCAATTTTTTGCCCCACCAAAACCAAAAA                                                                                                                               >  SRR19973071.1630166/1‑150 (MQ=26)
                          GTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCC                                                                                                                            >  SRR19973071.4324178/1‑150 (MQ=32)
                            CATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGC                                                                                                                          <  SRR19973071.2968918/150‑1 (MQ=32)
                            CATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGC                                                                                                                          <  SRR19973071.2968909/150‑1 (MQ=32)
                            CATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTATTCCCCGCCAAAAGCAAAAACCCGC                                                                                                                          >  SRR19973071.3775240/1‑150 (MQ=30)
                             ATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCC                                                                                                                         <  SRR19973071.214371/150‑1 (MQ=32)
                             ATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCC                                                                                                                         <  SRR19973071.694041/150‑1 (MQ=32)
                                 taaTTTCTGCCACTCAAAAATTAAAAACCCGCCGTAGCGTTTTTTTACGTAAAACATGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCAAAAAGCAAAAACCCGCCGAAG                                                                                                                     <  SRR19973071.420256/147‑1 (MQ=23)
                                 ATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG                                                                                                                     <  SRR19973071.128914/150‑1 (MQ=32)
                                 ATCTTTCTGCCCCTACAAATTTAAAAACCCGCCGAAGCGGTTTTTTACGTAAAACAGGTTAATCTGACCGATAAGCCGCTTTCTTTTGGTTTTAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG                                                                                                                     <  SRR19973071.420110/150‑1 (MQ=25)
                                   CTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG                                                                                                                   >  SRR19973071.1075249/1‑150 (MQ=32)
                                     TTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGG                                                                                                                 <  SRR19973071.155229/150‑1 (MQ=32)
                                     TTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGG                                                                                                                 <  SRR19973071.155301/150‑1 (MQ=32)
                                         GCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATATTGTCGTGGACCGGCATTGATTTTTATGCCCCTCCCAAAGCAATAACCCGCCGAAGCGGGTTTT                                                                                                             >  SRR19973071.2058029/1‑150 (MQ=25)
                                           CCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTA                                                                                                           <  SRR19973071.2687310/150‑1 (MQ=32)
                                            CCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGGCATTCCTTTTTCTGCCCCTGCAAAAGCAAAATCCCGCCGAAGCGGGTTTTGAC                                                                                                          >  SRR19973071.3523753/1‑150 (MQ=28)
                                             CTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACG                                                                                                         <  SRR19973071.4128755/150‑1 (MQ=32)
                                                 AAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGTCAGTCAATCAATTTTATTCCCCTCCAAAAGAAAAAAACCGCCGAAGCGGGTTTTTAAGTAAA                                                                                                     >  SRR19973071.1651553/1‑150 (MQ=25)
                                                  AAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAC                                                                                                    <  SRR19973071.2046958/150‑1 (MQ=34)
                                                     GTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAG                                                                                                 <  SRR19973071.2658636/150‑1 (MQ=34)
                                                      TAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGTGTCGTGGACAGTCCTTAATTTTTCTGCCCCTCCAAAATCAAAAACCCGCCGCAGCGGGGTTTTTCGGAAAGCAGG                                                                                                >  SRR19973071.1435348/1‑150 (MQ=18)
                                                       AAAAACCCGCCGAAGCGGGTTTTTACGTAAAACCGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGT                                                                                               <  SRR19973071.1655549/150‑1 (MQ=31)
                                                       AAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGT                                                                                               <  SRR19973071.4029161/150‑1 (MQ=31)
                                                       AAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGT                                                                                               >  SRR19973071.66190/1‑150 (MQ=32)
                                                            CCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAC                                                                                          <  SRR19973071.2804325/150‑1 (MQ=32)
                                                            CCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAC                                                                                          <  SRR19973071.2804324/150‑1 (MQ=32)
                                                            CCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAGAC                                                                                          >  SRR19973071.2393773/1‑150 (MQ=20)
                                                                  GTAGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCG                                                                                    <  SRR19973071.2101836/150‑1 (MQ=30)
                                                                  GAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCG                                                                                    <  SRR19973071.2101675/150‑1 (MQ=32)
                                                                  GAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCG                                                                                    <  SRR19973071.2101608/150‑1 (MQ=32)
                                                                   ttgcggttttttacgtatatctggttaatgTGACCGATTAGCCTCTTTCTTTTGTGTATAGTGTCGTGGACAGTAATTCATTTTTCTGCCCCTCCAAAATAAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGA                                                                                   <  SRR19973071.2442158/120‑1 (MQ=6)
                                                                   AAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGA                                                                                   >  SRR19973071.4762064/1‑150 (MQ=32)
                                                                   AAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAACTTTCTGCCCCTCCAAAAGCAAAAAACCGCCCAAGCGGGTTTTTACGTAAAACAGGTGAAACAGACCGA                                                                                   >  SRR19973071.3331140/1‑150 (MQ=19)
                                                                    AGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAACCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGAT                                                                                  <  SRR19973071.774920/150‑1 (MQ=31)
                                                                    AGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGAT                                                                                  <  SRR19973071.218020/150‑1 (MQ=32)
                                                                    AGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGAT                                                                                  <  SRR19973071.4363043/150‑1 (MQ=32)
                                                                      CGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAA                                                                                <  SRR19973071.2200286/150‑1 (MQ=20)
                                                                      CGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAA                                                                                <  SRR19973071.2006354/150‑1 (MQ=31)
                                                                      CGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAA                                                                                <  SRR19973071.2200239/150‑1 (MQ=32)
                                                                       GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAATTTTATTCACCTACAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACAGACCGATAAG                                                                               >  SRR19973071.1127264/1‑150 (MQ=27)
                                                                         GTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACATTCATTAATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTTCGTAAAACAGGTGAAACTGACCGATAAGCC                                                                             >  SRR19973071.3191448/1‑150 (MQ=19)
                                                                         GTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCC                                                                             <  SRR19973071.2884949/150‑1 (MQ=32)
                                                                          TTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCG                                                                            <  SRR19973071.3322400/150‑1 (MQ=32)
                                                                          TTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCG                                                                            <  SRR19973071.3322287/150‑1 (MQ=32)
                                                                           TTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGCAGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAAACCCCCGCAGCCGGTTTTTACGTAAACCAGGTTAAAATGACCGATAAACCGC                                                                           >  SRR19973071.3611744/1‑150 (MQ=25)
                                                                           TTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACCGTCATTCATCTTTCTGCCCCACCCAAAGCAAAAAACCCCCGAAGCGGGTTTTTAAGTAAACCACGGTAAAATGACCGATAAGCCGC                                                                           >  SRR19973071.3611768/1‑150 (MQ=26)
                                                                           TTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGC                                                                           >  SRR19973071.368245/1‑150 (MQ=34)
                                                                            TTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCT                                                                          <  SRR19973071.4199022/150‑1 (MQ=36)
                                                                              TACGTAAATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTT                                                                        <  SRR19973071.3799750/150‑1 (MQ=7)
                                                                                CGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCT                                                                      <  SRR19973071.3859581/150‑1 (MQ=44)
                                                                                CGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCT                                                                      <  SRR19973071.3859408/150‑1 (MQ=44)
                                                                                 GTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTT                                                                     <  SRR19973071.2359738/150‑1 (MQ=44)
                                                                                 GTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAAAGACCGATAAGCCGCTTTCTT                                                                     >  SRR19973071.2248489/1‑150 (MQ=42)
                                                                                   acatCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTT                                                                   <  SRR19973071.2174326/146‑1 (MQ=0)
                                                                                   AAATCAGGTTAAACTGACAGATAAGCAGCTTTCTTTTGTGTATAGTGTCGTGGACATTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGTGTTTTTACGTAAACCATGTGAAACTGACCGATAAGCCGCTTTCTTTT                                                                   <  SRR19973071.3844882/150‑1 (MQ=14)
                                                                                   AAAACCGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTT                                                                   <  SRR19973071.3845197/150‑1 (MQ=31)
                                                                                   AAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTT                                                                   >  SRR19973071.3956019/1‑150 (MQ=48)
                                                                                   AAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCAGCCCCAACAAAAGCAAAAACCCCCCGAAGCGGGTTTTTAAGTAAAACAGGTGAAACTTACCCATAAGCCGCTTTGTTTT                                                                   >  SRR19973071.73755/1‑150 (MQ=15)
                                                                                   AAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTATGCCCCTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTTAGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTATTTT                                                                   >  SRR19973071.72976/1‑150 (MQ=33)
                                                                                   AAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTT                                                                   >  SRR19973071.774363/1‑150 (MQ=47)
                                                                                    AACCAGGTTAAACTGACCGAGAAGCCGCTTTCTTTTGGGGATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTG                                                                  <  SRR19973071.1324091/150‑1 (MQ=19)
                                                                                    AAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTG                                                                  <  SRR19973071.1324520/150‑1 (MQ=38)
                                                                                     AACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGG                                                                 <  SRR19973071.4596566/150‑1 (MQ=36)
                                                                                        agctcAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAACAGGAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTA                                                              <  SRR19973071.1749873/145‑1 (MQ=7)
                                                                                        AGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTGTGGGGA                                                              >  SRR19973071.2695146/1‑150 (MQ=21)
                                                                                            GAAACTGACCGATAAGCCGCTTTCTTTTGGGTATCGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGC                                                          <  SRR19973071.3842555/150‑1 (MQ=25)
                                                                                            GAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGC                                                          <  SRR19973071.224525/150‑1 (MQ=25)
                                                                                            GAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGC                                                          <  SRR19973071.224529/150‑1 (MQ=25)
                                                                                            GAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGC                                                          <  SRR19973071.3842546/150‑1 (MQ=25)
                                                                                              AACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTTCCCCGCCAAAATCAAAAAACCGCCGAATCGGGTTTTTACGTAAACCAGGTGAAACTGTCCGATAAGCCGCTTTATTTTGGGTGTAGCGT                                                        >  SRR19973071.4244088/1‑150 (MQ=20)
                                                                                                CTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATCTTTATGCCCCTCCAAAAGCAAAAAACCGCCCAAGCCGGGTTTTACGTAAAACAGGTGTAACTGACCGAaaaaccccgttattttttgtatttcgtcg                                                      >  SRR19973071.1787583/1‑121 (MQ=0)
                                                                                                 TGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGT                                                     <  SRR19973071.3419591/150‑1 (MQ=32)
                                                                                                  GACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTG                                                    <  SRR19973071.20946/150‑1 (MQ=32)
                                                                                                  GACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTG                                                    <  SRR19973071.21055/150‑1 (MQ=32)
                                                                                                  GACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTG                                                    <  SRR19973071.605347/150‑1 (MQ=32)
                                                                                                        TAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAATTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTAGGGTATAGCGTATTGGACAGT                                              >  SRR19973071.1125800/1‑150 (MQ=24)
                                                                                                         AAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATTTTTATTCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAATCCGCTTTCTTTTGGGTATAGCGTCGTGGACAATC                                             >  SRR19973071.2625697/1‑150 (MQ=6)
                                                                                                          AGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCA                                            <  SRR19973071.4635540/150‑1 (MQ=32)
                                                                                                           GCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAAATTTTATGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAACGATAATCCGCTTTCTTTTGGGTATAGCGTCGTGGTCAGTCca                                           >  SRR19973071.3516828/1‑148 (MQ=26)
                                                                                                             CGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACAGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCcatc                                         >  SRR19973071.2081342/1‑146 (MQ=7)
                                                                                                              GCTTTCTTTTGGGTATAGTGTCGTGGAAAGTCATTAATTTTTATTCCCCTCCAAAAACAAAAAACCGCCGAAGCGGGTTTTTAAGTAAAACAGGTGAAACTGTACCATAAGCCGCTTTATTTTGGGGATATAGTCGTGGACAGTgaataa                                        >  SRR19973071.79353/1‑144 (MQ=0)
                                                                                                               CTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCGCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTTGGTGTAGCGTCGTGGACAGTCATTGAT                                       >  SRR19973071.2548557/1‑150 (MQ=6)
                                                                                                                 TTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCT                                     <  SRR19973071.3927995/150‑1 (MQ=60)
                                                                                                                  TCTTTTGGGTATAGTGTCGTGGTCAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTT                                    <  SRR19973071.4358785/150‑1 (MQ=31)
                                                                                                                  TCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTT                                    <  SRR19973071.4302469/150‑1 (MQ=32)
                                                                                                                     TTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCT                                 <  SRR19973071.3984780/150‑1 (MQ=32)
                                                                                                                     TTTGGGTATAGTGTCGCGGAAACTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGTAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCT                                 <  SRR19973071.247239/150‑1 (MQ=28)
                                                                                                                           TAGAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCT                           <  SRR19973071.29967/150‑1 (MQ=31)
                                                                                                                              AGTGTCGTGGACAGTCCTTCATTTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAgtaatatttcagccccacca                        >  SRR19973071.1101785/1‑130 (MQ=31)
                                                                                                                              AGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTagtggccaggccatcaaatttcggccccacca                        >  SRR19973071.1101824/1‑118 (MQ=60)
                                                                                                                               GTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAA                       >  SRR19973071.2707028/1‑150 (MQ=31)
                                                                                                                                TGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAA                      <  SRR19973071.1774136/150‑1 (MQ=32)
                                                                                                                                  TCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGTTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAG                    <  SRR19973071.3217975/150‑1 (MQ=31)
                                                                                                                                  TCGTGGACAGTCATTAATTTTTCTGCCACTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAG                    <  SRR19973071.1901273/150‑1 (MQ=30)
                                                                                                                                   CGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAATC                   >  SRR19973071.774831/1‑150 (MQ=31)
                                                                                                                                   CGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGC                   >  SRR19973071.774649/1‑150 (MQ=32)
                                                                                                                                     TGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAA                 >  SRR19973071.4509265/1‑150 (MQ=32)
                                                                                                                                     TGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAA                 >  SRR19973071.103594/1‑150 (MQ=31)
                                                                                                                                      GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCGTTCTGCCCCTCCAAAAGCAAA                >  SRR19973071.2625435/1‑150 (MQ=32)
                                                                                                                                       GACAGTCATTCTTTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAA               <  SRR19973071.3167624/150‑1 (MQ=31)
                                                                                                                                       GACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAATCAAAA               >  SRR19973071.4737626/1‑150 (MQ=30)
                                                                                                                                         CAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGTATAGCGTCGTGGAAAGTCATTCATAATTAAGCCCCCACAAAAGCAAAAAC             >  SRR19973071.4734093/1‑150 (MQ=55)
                                                                                                                                         CAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTGTCTTTTGGGTCTAGCGTCGTGGACAGTCATTCATCTTTCAGCCCCTCCAAAAGCAAAAAC             >  SRR19973071.4734098/1‑150 (MQ=29)
                                                                                                                                          AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACC            <  SRR19973071.1847066/150‑1 (MQ=32)
                                                                                                                                          AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACC            >  SRR19973071.1048700/1‑150 (MQ=32)
                                                                                                                                             CATTCTTTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGTAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGC         <  SRR19973071.2488217/150‑1 (MQ=30)
                                                                                                                                             CATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGC         <  SRR19973071.2488437/150‑1 (MQ=32)
                                                                                                                                              TTTCATTTTTCTGCCCCTCAAAAAGGTAAAACCAGCCGACGCGGGTTTTTACGTTAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCC        <  SRR19973071.1517000/150‑1 (MQ=18)
                                                                                                                                              ATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCC        <  SRR19973071.357747/150‑1 (MQ=32)
                                                                                                                                                   TTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGC   <  SRR19973071.2442001/150‑1 (MQ=32)
                                                                                                                                                    TTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG  <  SRR19973071.3592222/150‑1 (MQ=32)
                                                                                                                                                    TTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG  <  SRR19973071.3593098/150‑1 (MQ=21)
                                                                                                                                                    |                                                                                                                                                     
TTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG  >  CP009273/3263261‑3263558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑