Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,263,409 C→T 100% intergenic (+448/+166) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,263,4090CT91.7% 393.2 / 21.0 183intergenic (+448/+166)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base C (5/10);  new base T (66/95);  total (77/106)
Fisher's exact test for biased strand distribution p-value = 7.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 186 total aligned reads displayed.

TTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGC  >  CP009273/3263264‑3263557
                                                                                                                                                  |                                                                                                                                                    
ttttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTc                                                                                                                                                  <  2:1204751/150‑1 (MQ=37)
     ggTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGccc                                                                                                                                             <  2:4587184/150‑1 (MQ=37)
        ataGTGTCGTGGACAGTCATTCATCGTTCTGCCCCTCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAAAGTCATTCATTTTTATGccccga                                                                                                                                          >  1:1227242/1‑148 (MQ=255)
         taGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTcc                                                                                                                                         <  1:2230126/150‑1 (MQ=37)
                gTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCa                                                                                                                                  <  2:3119294/150‑1 (MQ=37)
                     cAGTCATTCATCTTTCTGCCCCTCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAAc                                                                                                                             <  1:1758424/150‑1 (MQ=37)
                       gTCATTCATCTTTCTGCCCCTCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAAccc                                                                                                                           <  2:550818/150‑1 (MQ=37)
                       gTCATTCATCTTTCTGCCCCTCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAAccc                                                                                                                           <  1:2462744/150‑1 (MQ=37)
                       gTCATTCATCTTTCTGCCCCTCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGAGGACAGTCAATCATTATACTGCCCCTCCAAAATCAAAAAacc                                                                                                                           >  2:3400851/1‑150 (MQ=11)
                       gTCATTCATCTTTCTGCCCCTCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTGTGGGGGATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCGAAAGCaaatcccc                                                                                                                           >  2:3400945/1‑145 (MQ=11)
                          attcatCTTTCTGCCCCCCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                                                                                                        <  2:2044752/150‑1 (MQ=255)
                            tcatCTTTCTGCCCCTCCAAATGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGTGTCGTGGAGAGTCAGTCATGTTTCTGCGCCTGCAAAAGCACAAACccgccgt                                                                                                                      >  2:5321633/1‑149 (MQ=11)
                            tcatCTTTCTGCCCCTCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGa                                                                                                                      >  1:2906558/1‑150 (MQ=37)
                              atCTTTCTGCCCCTCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGGGTCGGGGTGGGTAATTCATTTTTGTGCCCCTCCAAAAGCAAAAACCCGCCGAAg                                                                                                                    >  2:1545627/1‑150 (MQ=11)
                                   tCTGCCCCTCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGt                                                                                                               <  2:1332526/150‑1 (MQ=37)
                                    cTGCCCCTCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtt                                                                                                              <  1:4127263/150‑1 (MQ=37)
                                     tggcacTCCACAAGTAA‑AAACCCGCCGAATCGGGTTTTTACGTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                             <  1:3462271/145‑1 (MQ=11)
                                     tGCCCCTCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTAGGCCCCTCCAAAAGCAAAAACCCCCCGAAGCGGGgtt                                                                                                             >  2:4465532/1‑150 (MQ=11)
                                     tGCCCCTCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATGTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                             >  2:966592/1‑150 (MQ=21)
                                      gCCCCTCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                                                                                                            <  2:1804635/150‑1 (MQ=37)
                                        cccACCAAAAGTAC‑AAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAGTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                          <  2:2091218/150‑1 (MQ=11)
                                         cactcAAAAATAACAAACCCGCCGTAGCGGTTTTTTACGTACAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                          <  2:3095111/146‑1 (MQ=11)
                                            ccAACAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTa                                                                                                      <  2:4937632/150‑1 (MQ=255)
                                            ccAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACCGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTa                                                                                                      >  1:2052100/1‑150 (MQ=255)
                                            ccAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGTGTCGTGGCCAGTCATTCATTATTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTTCGTa                                                                                                      >  2:1342171/1‑150 (MQ=11)
                                               aaaGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAc                                                                                                   >  1:114847/1‑150 (MQ=38)
                                               aaaGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTGTTACGTAAAc                                                                                                   >  1:4362137/1‑150 (MQ=255)
                                               aaaGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGGCCTTCATTTTTCTGCCCCTCCGAAAGCAAAATCCCGCCGTCGCGGGTGTGTTCGTaata                                                                                                   >  2:4491954/1‑148 (MQ=11)
                                                aaGTAA‑AAACCCGCCGTAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTATGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAcc                                                                                                  <  2:2006467/150‑1 (MQ=255)
                                                    tataaaCCCGCCGTAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAgg                                                                                               <  2:637528/147‑1 (MQ=255)
                                                     a‑aaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTg                                                                                             <  2:2264870/150‑1 (MQ=37)
                                                        aaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                                                                                                            >  2:1059233/1‑133 (MQ=34)
                                                         aCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGTGTCGAGGAGAGTCCTTAATTTTTCTGCCCCTACAAAAGCAAAAAACCGCCGTAGCGGGTTTTTACGTGAAGCAGGTGaaa                                                                                          >  2:1912398/1‑150 (MQ=11)
                                                          cccGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAc                                                                                         <  2:2391169/150‑1 (MQ=17)
                                                          cccGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAAc                                                                                         >  1:4685616/1‑150 (MQ=17)
                                                           ccgccgTATCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACt                                                                                        <  2:4424331/150‑1 (MQ=11)
                                                             gccgAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa                                                                                      <  2:3589938/150‑1 (MQ=255)
                                                               cgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTTCGTAAAACAGGTGAAACTGAcc                                                                                    >  1:2444328/1‑150 (MQ=255)
                                                                gtaGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCg                                                                                   <  2:4458464/148‑1 (MQ=37)
                                                                gAATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCg                                                                                   <  2:2834421/150‑1 (MQ=255)
                                                                gAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATATTTCTGCCCCGCCAAAAGCAAAAAACCGCCGAAGCGGGTATTTACGTACAACAGGTGAAACAGACCg                                                                                   >  2:3653739/1‑150 (MQ=11)
                                                                    cGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTTAACCAGGTGAAACTGTCCGTTaa                                                                               >  1:3717655/1‑150 (MQ=255)
                                                                    cGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATaa                                                                               <  1:2151233/150‑1 (MQ=37)
                                                                     gggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACAGGACGATAAg                                                                              >  1:1743738/1‑150 (MQ=255)
                                                                      ggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTTGAAAGTCATTCATGTTTCTGCCCCACCAAAAGAAAAAACACGCCGAAGCGGGTTTTTACGTAAAAAAGGTGAAACAGACCGAGaaac                                                                             >  1:3225662/1‑148 (MQ=11)
                                                                          tttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGct                                                                         <  2:500412/150‑1 (MQ=38)
                                                                           ttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgggt                                                                        >  1:4790165/1‑147 (MQ=0)
                                                                           ttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctt                                                                        >  1:5209668/1‑150 (MQ=255)
                                                                             aCGTACAACATTTGACACTGAGCGATAAGCCGCTTTCTTTTGGTTATAGTGTCGTGGACAGTCTTTCTATTTACTGCCACTCCACAAGCAAAAACCCGCCGCAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttc                                                                      <  1:1514987/150‑1 (MQ=11)
                                                                              cGTAAAACAGGTTCAACTGACCGATAAGCCGCTGTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttct                                                                     <  2:4620214/150‑1 (MQ=255)
                                                                              cGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTTCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctgtct                                                                     >  1:2085735/1‑150 (MQ=255)
                                                                              cGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttct                                                                     <  1:557859/150‑1 (MQ=255)
                                                                               gTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttctt                                                                    <  1:1118949/150‑1 (MQ=255)
                                                                                 aaaaCAGGTGAAACTGACCGCTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACCGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                  <  2:5022235/150‑1 (MQ=255)
                                                                                 aaaaCAGGTGAAACTGACCGATCAGCCGCTTTCTTTTGGCTATAGTGTCGTGGACAGTCATTCATTTTTCGGCCCCGCCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtatt                                                                  <  2:253390/150‑1 (MQ=14)
                                                                                 aaaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                  <  1:3889614/150‑1 (MQ=255)
                                                                                  aaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGCACTCCTTAATTTTTATGCCCCACCCAAAGCAAAAAACCGCCGAAGCGGGTTTTTAAGTAAAACAGGTGAAACTGTCCCATAAGCCGCGGTCTTTTg                                                                 >  1:4983138/1‑150 (MQ=11)
                                                                                   aaCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                <  2:1868485/150‑1 (MQ=25)
                                                                                      aGGTGAAACTGACCGATAAGCCGCTTTCTTGTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTGTggggga                                                             >  2:4837137/1‑148 (MQ=11)
                                                                                       ggTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtat                                                            <  1:1042840/150‑1 (MQ=17)
                                                                                         tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAg                                                          <  1:4197941/150‑1 (MQ=25)
                                                                                          gAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGc                                                         <  2:2906558/150‑1 (MQ=25)
                                                                                          gAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                                                                                                          <  1:1032817/85‑1 (MQ=33)
                                                                                             aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtc                                                      <  1:4991505/150‑1 (MQ=37)
                                                                                                gACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTg                                                   <  1:2473824/150‑1 (MQ=37)
                                                                                                 aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTgg                                                  <  1:840029/150‑1 (MQ=37)
                                                                                                   cGATAAGCCGCTTTCTTTTGGGTATCGTGTCGTGGACAGTCATACATTTTTCGGCCCCTCCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAc                                                <  2:3524396/150‑1 (MQ=11)
                                                                                                   cGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAc                                                <  1:4528957/150‑1 (MQ=37)
                                                                                                   cGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGGCATTCAATTTTCTGCCCCACCAAAAGCAAAAACCCCCCCAAGCGGGTTTTTACGGAAACCCGGTGAAACAGACCGAAAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAc                                                >  1:2465251/1‑150 (MQ=11)
                                                                                                    gATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTTACCGATAAGCCGCGTTCTTTTGGGTATAGCGTCGTGGACa                                               >  1:296037/1‑150 (MQ=255)
                                                                                                     aTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACAGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCTAGGACAg                                              >  2:3327955/1‑150 (MQ=11)
                                                                                                      tAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGt                                             <  1:664045/150‑1 (MQ=37)
                                                                                                        atccGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTATTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtca                                           <  1:4648568/148‑1 (MQ=37)
                                                                                                        agacGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCCTTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAACCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtca                                           <  2:1242972/147‑1 (MQ=255)
                                                                                                         gCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcat                                          <  1:772517/150‑1 (MQ=37)
                                                                                                         gCCGCGATCTTTTGGGTATAGTGTCGTGGACAGACTTTCTTTTTTCTGCCACTCAAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcat                                          <  2:1654740/150‑1 (MQ=11)
                                                                                                           cGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTTCCCCACCAAAAGCAAAAAACCCCCGAAGCGGGTTTTTAAGTAAACCAGGTGAAACTGAACGATAAGCCGCTTTCTTTAGGGGACAGAGTCATGGAAAGtaaatc                                        >  2:4079453/1‑145 (MQ=11)
                                                                                                                   tttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCt                                <  1:391863/150‑1 (MQ=37)
                                                                                                                    ttGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTg                               <  1:1018954/150‑1 (MQ=37)
                                                                                                                    ttGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCGTTGGGGTATAGCGTCGTGGACAGTCATTGCTCGTTCTg                               >  1:3977915/1‑150 (MQ=11)
                                                                                                                    ttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                             >  2:5023720/1‑72 (MQ=34)
                                                                                                                    ttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                             <  1:5023731/72‑1 (MQ=34)
                                                                                                                    ttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                             >  2:5023731/1‑72 (MQ=34)
                                                                                                                        gTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGTATAGCGTCGTGGCCAGGCATACATAGTTCTGcccc                           >  2:377580/1‑150 (MQ=11)
                                                                                                                               gtCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTGTTACGTAAACCAGGTGAAACTGTCCGATAAGCCGCGGTCTGTTGGGGCTAGCGTCGGGGCCAGGCCTGCATGTTACTGccccggcagtc                    >  2:160547/1‑143 (MQ=11)
                                                                                                                                  gTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTAGAGCGTCGTGGGCAGTCATTGATGGTGCTGCGCCGGCAAAAGCa                 >  1:4968563/1‑150 (MQ=11)
                                                                                                                                  gTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTTAAAATGACCGATAAGCCGCTTTCTTTAGGGTATAGCGTCGTGGACAGTCATTCAACTTTCTGCACCTCCAAAAGCa                 >  2:4612499/1‑150 (MQ=11)
                                                                                                                                   tGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCaa                >  1:3348087/1‑150 (MQ=255)
                                                                                                                                   tGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGTATAGCGTCGTGGCCAGGCAAGCAAGTTGAGGCCCCACCAAAAGCaa                >  2:4026234/1‑150 (MQ=11)
                                                                                                                                     gACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGACCGTCCATCATGGTTATGCCCCGCCAAAAGccata              >  2:914383/1‑146 (MQ=11)
                                                                                                                                        aGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCATTCGGCCCCAGCCACAGCAAAAAcc           >  2:3157892/1‑150 (MQ=11)
                                                                                                                                         gTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGCCAGGCATTCAACAGTCTGCCCCGCCCACAGCCCAAAccc          >  1:2701975/1‑150 (MQ=11)
                                                                                                                                          tcattcatTTTTCTGCCCCTCCAAAAGCAAAATCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACccg         >  2:1954290/1‑150 (MQ=255)
                                                                                                                                           cattcattTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgc        >  2:2026155/1‑150 (MQ=37)
                                                                                                                                            attcatTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTGGGGGATAGAGTCTATGACAGTCCATCAAAATTAAGCCCCAACAAAATCAAAAACccgcc       >  1:2585416/1‑150 (MQ=11)
                                                                                                                                             ttcatTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTATTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTGTAGGGTAGAGCGACGAGGAAAGTCATTCATATTACAGCACCTGCAAGAACAAAAACccgccg      >  2:3457935/1‑150 (MQ=11)
                                                                                                                                               catTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTTCCCCTACAAAAGCAAAAACCCGCCGaa    >  2:2660038/1‑150 (MQ=255)
                                                                                                                                               catTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCACCTCCAAAAGCAAAAACccgccgca    >  2:2778763/1‑148 (MQ=255)
                                                                                                                                               catTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGTATAGCGTCGTGGACAGGCAGTCATCGTTCTGCCCCGCCAAAAGCAAAAACccgccgca    >  2:4223173/1‑148 (MQ=11)
                                                                                                                                                 tCTTTCTGCCCCTCCAACAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGc  <  2:608781/150‑1 (MQ=37)
                                                                                                                                                  |                                                                                                                                                    
TTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAA‑AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGC  >  CP009273/3263264‑3263557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG  >  CP009273/3263268‑3263558
                                                                                                                                                 |                                                                                                                                                     
GGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCC                                                                                                                                               <  SRR19973062.113214/150‑1 (MQ=32)
 GGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCC                                                                                                                                              <  SRR19973062.1545009/150‑1 (MQ=32)
    ATAGTGTCGTGGACAGTCATTCATCGTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAA‑‑‑‑AGTCATTCATTTTTATGCCCCga                                                                                                                                           >  SRR19973062.1232942/1‑148 (MQ=29)
     TAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCC                                                                                                                                          <  SRR19973062.2241044/150‑1 (MQ=32)
     TAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCC                                                                                                                                          <  SRR19973062.2241287/150‑1 (MQ=32)
      AGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCA                                                                                                                                         <  SRR19973062.2937061/150‑1 (MQ=32)
       GTGTCGTGGACAGTCATTCATCTTTCTGCCACTCCAAAAGTAAAACCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAA                                                                                                                                        <  SRR19973062.2050773/150‑1 (MQ=30)
            GTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACCTTAAGTCAATTATCTT‑‑‑‑CCCCTCCAAAAGCA                                                                                                                                   >  SRR19973062.3779862/1‑150 (MQ=27)
             TGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGCC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAA                                                                                                                                  >  SRR19973062.4142959/1‑150 (MQ=31)
                 CAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAAC                                                                                                                              <  SRR19973062.1766952/150‑1 (MQ=32)
                   gtgttttATCTTTCTGCCACTCAAAAATTAAAAACCCGCCGTAGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCC                                                                                                                            <  SRR19973062.3369670/143‑1 (MQ=28)
                   GTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCC                                                                                                                            <  SRR19973062.2475948/150‑1 (MQ=32)
                   GTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCC                                                                                                                            <  SRR19973062.2475796/150‑1 (MQ=32)
                      ATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTAATTTTTCTGCCCCGCCACAAGCAAAAACCCGCC                                                                                                                         >  SRR19973062.3653424/1‑150 (MQ=29)
                        TCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGA                                                                                                                       >  SRR19973062.2921852/1‑150 (MQ=32)
                        TCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTATTCCCCTCCAAAAGCAAAAAACCGCCGA                                                                                                                       >  SRR19973062.2921889/1‑150 (MQ=29)
                        TCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTATGCCCCACCAAAAGCAAAAACCCGCCGA                                                                                                                       >  SRR19973062.1577318/1‑150 (MQ=30)
                            CTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG                                                                                                                   >  SRR19973062.2612668/1‑150 (MQ=32)
                            CTTTCTGCCCCACCAAAAGTAAAAACCCGCCGAAGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG                                                                                                                   <  SRR19973062.4046134/150‑1 (MQ=30)
                                CTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTT                                                                                                               <  SRR19973062.4148625/150‑1 (MQ=32)
                                 tggcaCTCCACAAGTAAAAACCCGCCGAATCGGGTTTTTACGTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTT                                                                                                              <  SRR19973062.3479909/145‑1 (MQ=29)
                                  gcccccccatcagctAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑‑‑‑AGTCATTCATCTTTCTGCCCCTCCAAACGCAAAAACCCGCCGAAGCGGGTTTT                                                                                                             <  SRR19973062.1064213/135‑1 (MQ=0)
                                  GCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTT                                                                                                             <  SRR19973062.5278676/150‑1 (MQ=32)
                                   CCCCTCCACAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTT                                                                                                            <  SRR19973062.3966588/150‑1 (MQ=31)
                                   CCCCTCCAAAAGTAAAAACCCGCCGTAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTT                                                                                                            <  SRR19973062.81471/150‑1 (MQ=31)
                                   CCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTT                                                                                                            <  SRR19973062.4463007/150‑1 (MQ=32)
                                   CCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTAATTTTTATGCCCCTCCAAAAGCAAAAACCCGCCGACGCGGGTTTTT                                                                                                            >  SRR19973062.4234769/1‑150 (MQ=29)
                                    CCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGTC‑‑‑‑AGTCATTAATTTTTATTCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTA                                                                                                           >  SRR19973062.1994591/1‑150 (MQ=27)
                                     ccaccaaacgaacAAACCCGCCGAAGCGGGATTTTACGTAAAACTGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAC                                                                                                          <  SRR19973062.4974258/137‑1 (MQ=21)
                                      CTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACG                                                                                                         <  SRR19973062.643851/150‑1 (MQ=32)
                                        CCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑CGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTA                                                                                                       >  SRR19973062.2062608/1‑150 (MQ=31)
                                          AAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAA                                                                                                     <  SRR19973062.2392972/150‑1 (MQ=32)
                                           AAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAC                                                                                                    >  SRR19973062.115561/1‑150 (MQ=34)
                                           AAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTGTTACGTAAAC                                                                                                    >  SRR19973062.4384531/1‑150 (MQ=33)
                                                  AAACCCGCCGTAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGA                                                                                             <  SRR19973062.3548053/150‑1 (MQ=31)
                                                     CCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAC                                                                                          <  SRR19973062.3211992/150‑1 (MQ=32)
                                                     CCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAAC                                                                                          >  SRR19973062.4710105/1‑150 (MQ=20)
                                                       CGCCGAAGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTG                                                                                        <  SRR19973062.2324519/150‑1 (MQ=31)
                                                          ctatgctGGTTTTTACTTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACC                                                                                     <  SRR19973062.480222/143‑1 (MQ=31)
                                                          CGTAGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACC                                                                                     <  SRR19973062.3584179/150‑1 (MQ=30)
                                                          CGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTTCGTAAAACAGGTGAAACTGACC                                                                                     >  SRR19973062.2457440/1‑150 (MQ=20)
                                                          CGAAGCGGGTTATTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACC                                                                                     <  SRR19973062.4729878/150‑1 (MQ=31)
                                                           GAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCG                                                                                    <  SRR19973062.3848891/150‑1 (MQ=32)
                                                               CGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTTAACCAGGTGAAACTGTCCGTTAA                                                                                >  SRR19973062.3736728/1‑150 (MQ=29)
                                                               CGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAA                                                                                <  SRR19973062.2162136/150‑1 (MQ=32)
                                                                GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACAGGACGATAAG                                                                               >  SRR19973062.1752216/1‑150 (MQ=30)
                                                                 GGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTTGAA‑‑‑‑AGTCATTCATGTTTCTGCCCCACCAAAAGAAAAAACACGCCGAAGCGGGTTTTTACGTAAAAAAGGTGAAACAGACCGAgaaac                                                                              >  SRR19973062.3242405/1‑145 (MQ=17)
                                                                  GTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTGAATTTTATTCCCCACCAAAAGCAAAAAACCGCCCAAGCCGGGTTTTTCGTAAACCccgtgaaaaaggccgataaacc                                                                             >  SRR19973062.4563987/1‑128 (MQ=18)
                                                                   TTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑CGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAATGGCCGATAAGCCA                                                                            >  SRR19973062.2141564/1‑150 (MQ=29)
                                                                    TTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTGTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTAAACCAGGTGAAACAGACCGATAAGCCGC                                                                           >  SRR19973062.5057800/1‑150 (MQ=31)
                                                                    TTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGGAAACCAGGTGAAACTGACCGATAAGCCGC                                                                           >  SRR19973062.5057819/1‑150 (MQ=33)
                                                                      TTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTT                                                                         >  SRR19973062.5237146/1‑150 (MQ=38)
                                                                      TTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTT                                                                         >  SRR19973062.2092812/1‑150 (MQ=38)
                                                                      TTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGT                                                                         >  SRR19973062.4815032/1‑150 (MQ=33)
                                                                        ACGTACAACATTTGACACTGAGCGATAAGCCGCTTTCTTTTGGTTATAGTGTCGTGGAC‑‑‑‑AGTCTTTCTATTTACTGCCACTCCACAAGCAAAAACCCGCCGCAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTC                                                                       <  SRR19973062.1522451/150‑1 (MQ=15)
                                                                         CGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTTCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTGTCT                                                                      >  SRR19973062.2096400/1‑150 (MQ=34)
                                                                         CGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCT                                                                      <  SRR19973062.560489/150‑1 (MQ=44)
                                                                          GTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTT                                                                     <  SRR19973062.1124198/150‑1 (MQ=33)
                                                                          GTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTT                                                                     <  SRR19973062.1124147/150‑1 (MQ=44)
                                                                           tataaaatgtgatACTGAGCGATAATCCGCTTTCTTTTGGGTCTAGTGTCGCGGAC‑‑‑‑ACTGATTCAAATTTAGGCCCCTCCAAAAGCATAAACCCGCCGTATCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTT                                                                    <  SRR19973062.3260116/137‑1 (MQ=15)
                                                                            AAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTT                                                                   <  SRR19973062.5217103/150‑1 (MQ=29)
                                                                            AAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTT                                                                   <  SRR19973062.3909443/150‑1 (MQ=40)
                                                                            AAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTT                                                                   <  SRR19973062.4224687/150‑1 (MQ=40)
                                                                             AAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGC‑‑‑‑ACTCCTTAATTTTTATGCCCCACCCAAAGCAAAAAACCGCCGAAGCGGGTTTTTAAGTAAAACAGGTGAAACTGTCCCATAAGCCGCGGTCTTTTG                                                                  >  SRR19973062.5009440/1‑150 (MQ=11)
                                                                             AAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTATGCCCCACCAAAAACAAAAAACCCCCGAACCGGGTTGTTTCTGAAACCACGTGAAAATGACCGGTAAGCCGCgttggtttg                                                                  >  SRR19973062.5009457/1‑141 (MQ=13)
                                                                               ACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGG                                                                <  SRR19973062.4493408/150‑1 (MQ=22)
                                                                                  GGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAT                                                             <  SRR19973062.1047733/150‑1 (MQ=21)
                                                                                  GGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAT                                                             <  SRR19973062.1047707/150‑1 (MQ=29)
                                                                                    TGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAG                                                           <  SRR19973062.4219717/150‑1 (MQ=25)
                                                                                    TGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAG                                                           <  SRR19973062.4219709/150‑1 (MQ=25)
                                                                                     GAAACTGACCGATAAGCCGCTTTCTTTTGGGTATCGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGC                                                          <  SRR19973062.4363122/150‑1 (MQ=25)
                                                                                        ACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAATCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTC                                                       <  SRR19973062.2569226/150‑1 (MQ=29)
                                                                                        ACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTC                                                       <  SRR19973062.5017882/150‑1 (MQ=29)
                                                                                          TGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCTTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTTACCGATAATCCCCTTTATTTTGGGTATATCGTCGG                                                     >  SRR19973062.1216763/1‑150 (MQ=19)
                                                                                           GACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTG                                                    <  SRR19973062.2487075/150‑1 (MQ=32)
                                                                                           GACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTG                                                    <  SRR19973062.2479984/150‑1 (MQ=32)
                                                                                            ACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGG                                                   <  SRR19973062.843690/150‑1 (MQ=32)
                                                                                              CGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC                                                 <  SRR19973062.4552513/150‑1 (MQ=32)
                                                                                              CGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC                                                 <  SRR19973062.4552375/150‑1 (MQ=32)
                                                                                              CGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGGCATTCAATTTTCTGCCCCACCAAAAGCAAAAACCCCCCCAAGCGGGTTTTTACGGAAACCCGGTGAAACAGACCGAAAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC                                                 >  SRR19973062.2478463/1‑150 (MQ=25)
                                                                                               GATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTTACCGATAAGCCGCGTTCTTTTGGGTATAGCGTCGTGGACA                                                >  SRR19973062.297464/1‑150 (MQ=29)
                                                                                                 TAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGT                                              <  SRR19973062.667133/150‑1 (MQ=32)
                                                                                                   CGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAATCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCA                                            <  SRR19973062.2445325/150‑1 (MQ=31)
                                                                                                   ATCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTATTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCA                                            <  SRR19973062.4672885/150‑1 (MQ=31)
                                                                                                    GCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAT                                           <  SRR19973062.775981/150‑1 (MQ=32)
                                                                                                      CGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC                                         <  SRR19973062.490498/150‑1 (MQ=32)
                                                                                                       GCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAACCAGGTGAAACAGACCGATAAGCCGCGTTCTTTTGGGTAaagagacgtatacagtcaatca                                        >  SRR19973062.1553051/1‑128 (MQ=20)
                                                                                                       GCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCACCAAAATCAAAAACCCCCCGAAGCGGGTTTTTACGTAAAACACGTGAAACAGACCGATAATCCGCGTTATTTTGGGTATAGactagtttacagtcaatca                                        >  SRR19973062.1883539/1‑131 (MQ=0)
                                                                                                        cgaTCTTTTGGGACTAGTGTCGTGGAC‑‑‑‑AGTCATTCAAATAACTCCCCCTCCAAAAGCAAAAACCCGCCGTAGCGGTTTTTTACGTACACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAT                                       <  SRR19973062.15389/147‑1 (MQ=25)
                                                                                                        CTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGaccatcaa                                       >  SRR19973062.2804747/1‑142 (MQ=7)
                                                                                                        CTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCAATCATTTTTATGCCCCACCAAAATCAAAAAACCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGGTAAGCCGCGTTCGTTTGGGTATAGCGTCGTTGAAAGTCATTaaa                                       >  SRR19973062.4682578/1‑147 (MQ=5)
                                                                                                         TTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCAATCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTGTTGGGgagagcggcggggcccggccatcatc                                      >  SRR19973062.716282/1‑124 (MQ=20)
                                                                                                            CTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTT                                   <  SRR19973062.3453332/150‑1 (MQ=32)
                                                                                                            CTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCAATTTTCTGCCCCTCCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTT                                   <  SRR19973062.534193/150‑1 (MQ=30)
                                                                                                            CTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCAATCAATTTTAATCCCCTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAAAAAGGTGAAACTGACCGATAAGACGCTTTaattagtgtaaatagtatatgaaaatacataaaagta                                   >  SRR19973062.1530289/1‑113 (MQ=0)
                                                                                                              TTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCT                                 <  SRR19973062.393606/150‑1 (MQ=60)
                                                                                                               TTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTG                                <  SRR19973062.1023730/150‑1 (MQ=32)
                                                                                                               TTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCGTTGGGGTATAGCGTCGTGGACAGTCATTgctcgttctg                                >  SRR19973062.3998050/1‑140 (MQ=30)
                                                                                                                 GGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCCGGTGGAACTGGCCGATAAGCCGCGTTCGTTTGGGGTTAGCGTCGTGGACcgggattgatgtgtatggc                              >  SRR19973062.1688568/1‑131 (MQ=30)
                                                                                                                     ATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTC                          <  SRR19973062.3400541/150‑1 (MQ=31)
                                                                                                                     ATAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCAAACAGCAAAAACCCGCCGAAGCGTGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTC                          <  SRR19973062.5318722/150‑1 (MQ=29)
                                                                                                                      TAGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCC                         <  SRR19973062.845220/150‑1 (MQ=32)
                                                                                                                       AGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCA                        <  SRR19973062.340193/150‑1 (MQ=32)
                                                                                                                       AGTGTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGgccggcattcatagttatgccccgaca                        >  SRR19973062.664043/1‑123 (MQ=32)
                                                                                                                          GTCGTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAA                     <  SRR19973062.1280445/150‑1 (MQ=32)
                                                                                                                             GTGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTAGAGCGTCGTGGGCAGTCATTgatggtgctgcgccggcaaaagca                  >  SRR19973062.4994724/1‑126 (MQ=47)
                                                                                                                              TGGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAA                 >  SRR19973062.3365342/1‑150 (MQ=31)
                                                                                                                               GGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAATCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAACTTTATGACCCACCAAAAGCAAA                >  SRR19973062.237383/1‑150 (MQ=27)
                                                                                                                               GGAC‑‑‑‑AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAA                >  SRR19973062.237346/1‑150 (MQ=32)
                                                                                                                                       AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACC            >  SRR19973062.3918962/1‑150 (MQ=32)
                                                                                                                                       AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTGTGGGGTATAGCGTCGTGGGCAGTCATTCATCTTTATGCCCCGCCAAAAGCAATAACC            >  SRR19973062.2811803/1‑150 (MQ=54)
                                                                                                                                       AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACAAGGTGAAACTGACCGATAAGCCGCTATCTGTTGGGTAGAGCGTCGTGGACAGTCATTCATAATACAGCCCCACCAAAAGCAAAAACC            >  SRR19973062.697173/1‑150 (MQ=47)
                                                                                                                                        GTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGTAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCC           <  SRR19973062.1971840/150‑1 (MQ=31)
                                                                                                                                        GTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGCCAGGCATTCAacagtctgccccgcccacagcccaaaccc           >  SRR19973062.2716150/1‑121 (MQ=54)
                                                                                                                                         TCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCAGCCCCTCCAAAAGCAAAAACCCG          >  SRR19973062.2653760/1‑150 (MQ=31)
                                                                                                                                         TCATTCATTTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCTGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCG          <  SRR19973062.1037651/150‑1 (MQ=20)
                                                                                                                                           CTTCATATTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCC        <  SRR19973062.1983956/150‑1 (MQ=0)
                                                                                                                                           ATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTgtgggggatagagtctatgacagtccatcaaaattaagccccaacaaaatcaaaaacccgcc        >  SRR19973062.2599133/1‑88 (MQ=28)
                                                                                                                                           ATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTTTAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCC        <  SRR19973062.258235/150‑1 (MQ=31)
                                                                                                                                            TTCATTTTTCTGCCCCTCCAATAGAAATTACCCGAGGAATCTGGTTTTTACTTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCA       <  SRR19973062.3286311/150‑1 (MQ=17)
                                                                                                                                               ATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCCTTCATCATTATGCCCCAACAAAATCAAAAACCCGCAGAAG    >  SRR19973062.1227683/1‑150 (MQ=60)
                                                                                                                                               ATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAG    >  SRR19973062.3572025/1‑150 (MQ=30)
                                                                                                                                               ATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTGTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGCAG    >  SRR19973062.4324091/1‑150 (MQ=30)
                                                                                                                                                 TTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCG  >  SRR19973062.5108262/1‑150 (MQ=31)
                                                                                                                                                 |                                                                                                                                                     
GGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑‑‑‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG  >  CP009273/3263268‑3263558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑