Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,263,409 C→T 100% intergenic (+448/+166) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,263,4090CT95.1% 253.0 / 5.5 109intergenic (+448/+166)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base T (43/59);  total (46/63)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 112 total aligned reads displayed.

TTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCC  >  CP009273/3263265‑3263552
                                                                                                                                                |                                                                                                                                               
tttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATtttt                                                                                                                                              <  2:2425087/148‑1 (MQ=37)
tttGGGTATAGTGTCGTGGACAGTCAGTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATtttt                                                                                                                                              <  2:2740795/148‑1 (MQ=255)
 ttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTc                                                                                                                                             <  2:3706332/148‑1 (MQ=255)
 ttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTc                                                                                                                                             <  2:3171720/148‑1 (MQ=37)
 gtGGGTATAGTGTCGTGGACACTCATTCATCTTACGGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTc                                                                                                                                             <  2:3706348/147‑1 (MQ=255)
       ataGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCGTTTGGGTATAGTGTCGTGGGCAGGGCTTGATTTTTCTGgccc                                                                                                                                       >  1:810820/1‑148 (MQ=11)
         aGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTc                                                                                                                                     <  2:3474747/148‑1 (MQ=37)
         aGTGTCGTGGACACTCATTCATCTTTCTGCCACTCAAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTc                                                                                                                                     <  2:952236/148‑1 (MQ=255)
              cGTGGACAGTGATTCATCTTTCTGCCCCTCCAAAATTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCaaaa                                                                                                                                <  1:1737506/148‑1 (MQ=255)
               gttgACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAg                                                                                                                               <  2:2070834/145‑1 (MQ=255)
                       tcattcatCTTTCTGCCCCTCCAAAATTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAAcc                                                                                                                       <  1:1629292/148‑1 (MQ=255)
                       tcattcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAAcc                                                                                                                       >  1:1127106/1‑148 (MQ=37)
                       tcattcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAAcc                                                                                                                       <  2:2043591/148‑1 (MQ=37)
                          ttcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTTACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGCAGTCATTAAATTTTCTGCCCCACCCAAAACAAAAAccccc                                                                                                                    >  1:1984573/1‑146 (MQ=11)
                               cTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAg                                                                                                               <  1:1853155/148‑1 (MQ=37)
                               cTTTCGGCCCCTCCAAAAGTAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAg                                                                                                               <  1:1853140/148‑1 (MQ=255)
                                 ttCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTTCGCCTCCAAAATCAAAAACCCCCCGCAGCg                                                                                                             >  1:2604916/1‑148 (MQ=11)
                                 ttCTGCCACTCCAAAAGTAAAAACCCGCCGAATCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCg                                                                                                             <  2:2724236/148‑1 (MQ=255)
                                  tccgCCCCTCCACAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                                            <  1:3140147/145‑1 (MQ=255)
                                   cTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCggg                                                                                                           <  2:1917577/148‑1 (MQ=37)
                                    tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGt                                                                                                          <  1:2926967/148‑1 (MQ=37)
                                    ggCCCCTCCACAAGTAAAAACCCGCCGAAGCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGt                                                                                                          <  1:2926898/147‑1 (MQ=255)
                                      ccccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAACAGCAAAAACCCGCCGAAGCGGGttt                                                                                                        <  2:2553797/148‑1 (MQ=255)
                                      ccccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                        <  2:1678778/148‑1 (MQ=37)
                                       cccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                                                                                                       <  1:2166141/148‑1 (MQ=37)
                                        ccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCCAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                                      >  1:2383787/1‑148 (MQ=255)
                                        ccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                                      <  2:3160023/148‑1 (MQ=37)
                                              caaGTAAAAACCCGCCGCAGCGGGTTTTTACGTAACACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaa                                                                                                <  2:800635/147‑1 (MQ=255)
                                                attAAAAACCCGCCGCATCGGTTTATTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAc                                                                                              <  2:2212720/146‑1 (MQ=11)
                                                aGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaaaa                                                                                              >  1:1577087/1‑147 (MQ=37)
                                                 gTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAcc                                                                                             <  1:3194708/148‑1 (MQ=38)
                                                 ctAAAAACCCGCCGAAGCGGTTTTTTACGTAAAACAGTTGAAACTGAACTATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTAATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAcc                                                                                             <  1:1035645/147‑1 (MQ=11)
                                                  tAAACACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCa                                                                                            <  2:2311735/148‑1 (MQ=255)
                                                   aaaaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg                                                                                           <  1:2369311/148‑1 (MQ=255)
                                                   aaaaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg                                                                                           <  1:2369388/148‑1 (MQ=37)
                                                   aaaaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg                                                                                           <  1:2369313/148‑1 (MQ=37)
                                                       aCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGa                                                                                       >  2:508824/1‑148 (MQ=37)
                                                         ccgccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaaa                                                                                     <  1:3662725/148‑1 (MQ=37)
                                                             cgcatcGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa                                                                                 <  2:3617639/143‑1 (MQ=255)
                                                             cgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa                                                                                 <  2:2882003/148‑1 (MQ=37)
                                                               aaGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc                                                                               >  2:129536/1‑148 (MQ=37)
                                                                aGCGGGTTAGTACGTACAACAGGTCAAACTGACCGATACGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCCCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCg                                                                              <  2:2120234/148‑1 (MQ=11)
                                                                 gCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGa                                                                             <  2:2383787/148‑1 (MQ=37)
                                                                      tttttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAATTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGc                                                                        >  2:3370264/1‑148 (MQ=12)
                                                                      tttttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCAATTTTGAGCCCCACCAAAAGCAAAAACCCGCCGAAGCAGGTTTTTACGGAAAACAAGTGAAACTGACCGATaatc                                                                        >  1:1516519/1‑146 (MQ=11)
                                                                       ttttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGGCCCTCCAAAAGCAAAAACCCCCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACAGACCCATAAGcc                                                                       >  1:2071045/1‑148 (MQ=11)
                                                                       ttttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATTATTCAGCCCCGCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTAAGGAAAACAGGTGAAACTGACCGATAAGcc                                                                       >  1:193241/1‑148 (MQ=11)
                                                                        tttACGTAAAACAGGTGAAACTGACCGATAATCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCTTTCCTTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGTAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCg                                                                      <  2:915975/148‑1 (MQ=11)
                                                                         ttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGc                                                                     <  2:2455521/148‑1 (MQ=255)
                                                                          tACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGct                                                                    <  2:3132442/148‑1 (MQ=38)
                                                                             gTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACTGTCATTATTTTTTCTGCCCCTACATAAGCACAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttc                                                                 <  1:2812703/148‑1 (MQ=11)
                                                                             gTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttc                                                                 <  1:3300769/148‑1 (MQ=255)
                                                                             gTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttc                                                                 <  1:3300603/148‑1 (MQ=255)
                                                                              tcaaaCACGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGctttct                                                                <  2:2347018/146‑1 (MQ=255)
                                                                              tAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACCGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTAAGTTAAACAGGTGAAACTGACCGATAAGCCGcgttct                                                                >  1:3472528/1‑148 (MQ=11)
                                                                                aaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttcttt                                                              <  2:3431997/148‑1 (MQ=255)
                                                                                  aCAGGTTAAACTGACCGATAAGCCTCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                            <  2:1300749/148‑1 (MQ=2)
                                                                                  aCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGCAGGCATTAATTTTTCTGCCCCTCCAAAAGCAAAAAACCCCCCAAGCGGGTTTTTAAGTAAAACAGGTGAAACTGACCGATAATCCGCGTTGTTTTg                                                            >  1:370874/1‑148 (MQ=11)
                                                                                   cAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGcgttctgtggg                                                           >  2:3332646/1‑143 (MQ=255)
                                                                                     ggTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgtgt                                                         >  2:3250697/1‑148 (MQ=255)
                                                                                        gAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                      <  2:1127106/148‑1 (MQ=25)
                                                                                        gAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                      <  1:70591/148‑1 (MQ=25)
                                                                                        caaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                      <  1:1686914/147‑1 (MQ=21)
                                                                                           aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCCTTCATTTTTAAGCCCCGCCAAAAGCAAAAACCCGCCCAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAATCCGCTTTCGTTTGGGGATAGcg                                                   >  1:2661909/1‑148 (MQ=11)
                                                                                           aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcg                                                   <  2:2068861/148‑1 (MQ=25)
                                                                                            cTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATTTTTCTGCCCCTCCAAAACCAAAAAACCCCCGAACCGGGGTTTTACGTAAACCAGGGGAAAATGACCGATAACCCGCGTTCGGTTGGGTGGAGcgg                                                  >  1:466655/1‑147 (MQ=11)
                                                                                              gACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAAcc                                                                                                                       >  1:3501796/1‑77 (MQ=21)
                                                                                              gACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcg                                                <  1:2631264/148‑1 (MQ=37)
                                                                                              gACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcg                                                >  1:3501825/1‑148 (MQ=37)
                                                                                               aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtatt                                               >  2:2468469/1‑145 (MQ=255)
                                                                                               aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGaa                                                                                                                >  2:2271753/1‑83 (MQ=33)
                                                                                                    gaaGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACa                                          <  2:3027211/147‑1 (MQ=37)
                                                                                                       gCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAATCCGCTTTCTTTTGGGTATAGAGTCGTGGCCAGtg                                       >  2:2082866/1‑147 (MQ=255)
                                                                                                       gCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAACCAGGTGAAACTGACCGATAAACCGCTTTCTTTTGGGGATAGCCTCGGGGACAGtc                                       >  2:3232846/1‑148 (MQ=11)
                                                                                                       gCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAATTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTATTTTGGGTATAGCGTCGTGGACAGtc                                       >  1:935281/1‑148 (MQ=255)
                                                                                                       gCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATTTTTCTGCCCCACCAAAATCAAAAAACCGCCCAACCGGGTTTTTAAGTAAACCACGTGAAACTGACCCATAAGCCGCGTTCTTTTGGGTATAGCGTAGTGGGCACtc                                       >  1:1136448/1‑148 (MQ=11)
                                                                                                       gCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTAATTCATTTTTGTGCCCCTCCAAAAGCAAATACCCGCCGGAGCGGGGTTTTACGTAAACCAGGTGTAACTGTCCGATAAGCCGGTTTGTTTTGGGGATAGCGTCGTGGACAGtg                                       >  1:935275/1‑147 (MQ=11)
                                                                                                         cGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTGTTGGGGATAGCGTGGTGGACAGtcat                                     >  2:2972287/1‑148 (MQ=11)
                                                                                                         cGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCCAAGCGGGTTTTTACGTAAAACAGGTGAAACAGACCGATAAGCCGCTTTCTTTTGGGTAGAGCGTAGTGGTCCGtcat                                     >  2:1064940/1‑148 (MQ=11)
                                                                                                         cGCCTTCTTTTGGTTATCGTCTCGTCGACAGTGCTTCAAATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGTTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcat                                     <  2:1766702/148‑1 (MQ=11)
                                                                                                          gCTTTCTTTTGGGTATAGTGTCGTGGACCGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAACCCGGTTTTTAAGTAAACCAGCTGAAACTGACCGAAAAGCCGCTTTCTTTTGGGTATAGCGTAGTGGCCACtcaag                                    >  1:2005551/1‑146 (MQ=11)
                                                                                                          gCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCCGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcatt                                    >  2:891426/1‑148 (MQ=255)
                                                                                                            tttctttTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattca                                  <  1:3313792/148‑1 (MQ=37)
                                                                                                            tttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCg                                                                                                             >  2:919859/1‑73 (MQ=34)
                                                                                                             ttctttTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattcct                                 >  2:3035312/1‑146 (MQ=37)
                                                                                                             ttctttTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCCCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcaataat                                 >  2:3217079/1‑143 (MQ=255)
                                                                                                               ctttTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCt                               <  1:1229652/148‑1 (MQ=17)
                                                                                                               ctttTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTACAAAAGCAAAAAACCCCCGAAGCGGGTTTTTACGTAAACCACGTGAAACTGAACGATAAGCCCCGTTCTTTTGGGGATAGGGTCGAGGAAAGtcaatcaaaa                               >  1:3285120/1‑145 (MQ=11)
                                                                                                                 tttGTGTATAGTGTCGTGTACAGTCATTCTTATTTCTGCCCCTCCAAAAGAACATACCCGTCGATGGGTTTTTTTACGTATCCCATGTGTAACTGACCGATTAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATCttt                             <  1:2133468/148‑1 (MQ=11)
                                                                                                                            tgtCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGCTAAGCCGCTTTCTTTTGGGTTTAGCGTCGTGGACAGGCAATCATATTTATTCCCCGCCa                  >  1:3750504/1‑148 (MQ=11)
                                                                                                                               cGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGCGTCGTGGACAGTCATTCATCATTATGCACCTCCaaaa               >  2:2293433/1‑148 (MQ=11)
                                                                                                                               cGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa               <  2:2584874/148‑1 (MQ=21)
                                                                                                                                   gACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAATCaa           >  2:2695833/1‑148 (MQ=255)
                                                                                                                                        tcattcatTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAcc      <  2:3501825/148‑1 (MQ=37)
                                                                                                                                        tcattcatTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAcc      >  2:596973/1‑148 (MQ=37)
                                                                                                                                        tcattcatTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGACAGGCATGCATCTGTCTGCCCCACCAAAAGCACTAAcc      >  2:596450/1‑148 (MQ=11)
                                                                                                                                        tcattcatTTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAcc      <  2:1612506/148‑1 (MQ=21)
                                                                                                                                         cattcattTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTCTAGCGTCGTGGACAGTCAATCATCTTTGTGCCCCTGCAAAAGCAAAAAccc     >  1:3389849/1‑148 (MQ=11)
                                                                                                                                            tcatTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc  >  1:3169761/1‑148 (MQ=37)
                                                                                                                                            tcatTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAATCAAAAACccgcc  >  2:2837417/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCC  >  CP009273/3263265‑3263552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGC  >  CP009273/3263261‑3263557
                                                                                                                                                    |                                                                                                                                                     
TTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTT                                                                                                                                                      <  SRR19973069.591464/150‑1 (MQ=60)
TTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTT                                                                                                                                                      <  SRR19973069.591223/150‑1 (MQ=60)
TTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTT                                                                                                                                                      <  SRR19973069.2521983/150‑1 (MQ=60)
  CTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑T                                                                                                                                                   <  SRR19973069.989757/150‑1 (MQ=32)
         GTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTATTTTGGGTATAGTGTCGTGGCCCGTCATTAATTTT‑TATGCCCC                                                                                                                                            >  SRR19973069.2031963/1‑150 (MQ=28)
                  CGTGGACAGTGATTCATCTTTCTGCCCCTCCAAAATTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGC                                                                                                                                   <  SRR19973069.1749697/150‑1 (MQ=30)
                        CAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCCTTCATTTT‑TGTTCCCCTCCAAAAGCAAAAAC                                                                                                                             >  SRR19973069.3565256/1‑150 (MQ=29)
                        CAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGTGTCGTGGACAGTCCTTCATTTT‑TCTGCCCCTCCCAAAGCAAAAAC                                                                                                                             >  SRR19973069.3565367/1‑150 (MQ=29)
                         AGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGCCAGTCATTCATTTT‑TATGCCCCTCCAAAAGCAAAAACC                                                                                                                            >  SRR19973069.2974605/1‑150 (MQ=30)
                         AGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAAAC                                                                                                                            >  SRR19973069.1134963/1‑150 (MQ=31)
                           TCATTCATCTTTCTGCCCCTCCAAAATTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCG                                                                                                                          <  SRR19973069.1640402/150‑1 (MQ=31)
                            CATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTTACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGCAGTCATTAAATTT‑TCTGCCCCACCCAAAACAAAAACCCCC                                                                                                                         >  SRR19973069.1998387/1‑150 (MQ=26)
                            CATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAAACCGC                                                                                                                         >  SRR19973069.206212/1‑150 (MQ=31)
                                 ATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGGCATTAATTTT‑TATGCCCCGCCAAAATCAAAAACCCGCCccag                                                                                                                    >  SRR19973069.1499166/1‑146 (MQ=60)
                                   CTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTTCGCCTCCAAAATCAAAAACCCCCCGCAGCG                                                                                                                  >  SRR19973069.2622314/1‑150 (MQ=28)
                                   CTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG                                                                                                                  <  SRR19973069.1866091/150‑1 (MQ=32)
                                   CTTTCGGCCCCTCCAAAAGTAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG                                                                                                                  <  SRR19973069.1866076/150‑1 (MQ=29)
                                      TCCGCCCCTCCACAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGT                                                                                                               <  SRR19973069.3160708/150‑1 (MQ=30)
                                        TGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTT                                                                                                             <  SRR19973069.2946366/150‑1 (MQ=32)
                                        TGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTT                                                                                                             <  SRR19973069.2945939/150‑1 (MQ=32)
                                        GGCCCCTCCACAAGTAAAAACCCGCCGAAGCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTT                                                                                                             <  SRR19973069.2946297/150‑1 (MQ=29)
                                          CCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCCAAAGCAAAAACCCGCCGGAGCGGGTTTTT                                                                                                           >  SRR19973069.2399951/1‑150 (MQ=30)
                                          CCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTT                                                                                                           <  SRR19973069.3343828/150‑1 (MQ=32)
                                           CCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTA                                                                                                          <  SRR19973069.2180949/150‑1 (MQ=32)
                                             CTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACG                                                                                                        <  SRR19973069.939904/150‑1 (MQ=32)
                                                  AAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAA                                                                                                   >  SRR19973069.1587800/1‑150 (MQ=31)
                                                     GTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAG                                                                                                <  SRR19973069.3215453/150‑1 (MQ=34)
                                                     CTAAAAACCCGCCGAAGCGGTTTTTTACGTAAAACAGTTGAAACTGAACTATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTAATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAG                                                                                                <  SRR19973069.1042835/150‑1 (MQ=27)
                                                      TAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGCAGTGATTCATTTT‑TCTGCCCCTCCAAAATCAAAAACCCCCCGAAGCGGGTTTTTTCGTAAAACAGG                                                                                               >  SRR19973069.2611768/1‑150 (MQ=20)
                                                      TAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAAGTT‑TATGCCCCTCCAAAAGCAAAAACCCCCCCAAGCGGGTTgtgacggtaagccgg                                                                                               >  SRR19973069.2799396/1‑135 (MQ=21)
                                                      TAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAAAGTCATTAATTTT‑TCTGCCCCACCAAAAGCAAAAACacgcagcaccggcgtttaaagtaaaccagg                                                                                               >  SRR19973069.3343487/1‑120 (MQ=60)
                                                       AAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGT                                                                                              <  SRR19973069.2385391/150‑1 (MQ=31)
                                                       AAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGTCAGTGATTGATTTT‑TCTGGCCCGCCCAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTgatcgaggt                                                                                              >  SRR19973069.1971318/1‑141 (MQ=18)
                                                       AAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGT                                                                                              <  SRR19973069.2385393/150‑1 (MQ=32)
                                                       AAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGT                                                                                              <  SRR19973069.2385468/150‑1 (MQ=32)
                                                          AACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAA                                                                                           >  SRR19973069.1289300/1‑150 (MQ=20)
                                                           ACCCGCCGCAGCGGGTTATTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAA                                                                                          <  SRR19973069.3477047/150‑1 (MQ=30)
                                                            CCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAC                                                                                         <  SRR19973069.21791/150‑1 (MQ=32)
                                                            CCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TATGCCCCTACAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAACCATGGGAAAC                                                                                         >  SRR19973069.3154615/1‑150 (MQ=24)
                                                             CCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACT                                                                                        <  SRR19973069.3686704/150‑1 (MQ=32)
                                                               GCCGTATCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGA                                                                                      <  SRR19973069.2188057/150‑1 (MQ=29)
                                                               GCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGA                                                                                      <  SRR19973069.2188056/150‑1 (MQ=32)
                                                               GCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGA                                                                                      <  SRR19973069.2188127/150‑1 (MQ=32)
                                                               GCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGA                                                                                      <  SRR19973069.2188071/150‑1 (MQ=32)
                                                                   AAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCCCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGA                                                                                  >  SRR19973069.2482863/1‑150 (MQ=31)
                                                                   AAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TATGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGA                                                                                  >  SRR19973069.3401412/1‑150 (MQ=31)
                                                                        GGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCAATTT‑TGAGCCCCACCAAAAGCAAAAACCCGCCGAAGCAGGTTTTTACGGAAAACAAGTGAAACTGACCGATAATC                                                                             >  SRR19973069.1526987/1‑150 (MQ=16)
                                                                         GTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGGCCCTCCAAAAGCAAAAACCCCCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACAGACCCATAAGCC                                                                            >  SRR19973069.2085396/1‑150 (MQ=28)
                                                                         GTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATTAT‑TCAGCCCCGCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTAAGGAAAACAGGTGAAACTGACCGATAAGCC                                                                            >  SRR19973069.194810/1‑150 (MQ=17)
                                                                              TACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTT                                                                       <  SRR19973069.68906/150‑1 (MQ=40)
                                                                                CGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACCGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTAAGTTAAACAGGTGAAACTGACCGATAAGCCGCGTTCT                                                                     >  SRR19973069.3494980/1‑150 (MQ=18)
                                                                                 GTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACTGTCATTATTTTT‑TCTGCCCCTACATAAGCACAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTT                                                                    <  SRR19973069.2831337/150‑1 (MQ=28)
                                                                                 GTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTT                                                                    <  SRR19973069.3322385/150‑1 (MQ=33)
                                                                                 GTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTT                                                                    <  SRR19973069.3322219/150‑1 (MQ=44)
                                                                                 GTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTT                                                                    <  SRR19973069.1542091/150‑1 (MQ=44)
                                                                                 GTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTAAGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTT                                                                    >  SRR19973069.3097775/1‑150 (MQ=31)
                                                                                    AAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGCAGGCATTAATTTT‑TCTGCCCCTCCAAAAGCAAAAAACCCCCCAAGCGGGTTTTTAAGTAAAACAGGTGAAACTGACCGATAATCCGCGTTGTTTTG                                                                 >  SRR19973069.373580/1‑150 (MQ=12)
                                                                                    AAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGGGTCGTGGTCCGTCAATCATTTT‑TCTGCCCCCCCCAAAGCAAAAACCCCCCGACGCGGGTTTTTAAGTAAAACAGGTGAAACTGACCGATAAGCCGCggtggttgg                                                                 >  SRR19973069.373466/1‑141 (MQ=3)
                                                                                            GAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGC                                                         <  SRR19973069.71397/150‑1 (MQ=25)
                                                                                            CAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGC                                                         <  SRR19973069.1698692/150‑1 (MQ=23)
                                                                                             AAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCCTTCATTTT‑TAAGCCCCGCCAAAAGCAAAAACCCGCCCAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAATCCGCTTTCGTTTGGGGATAGCG                                                        >  SRR19973069.2679751/1‑150 (MQ=20)
                                                                                              AACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATTTT‑TCTGCCCCTACAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAACCAGGGGAAACTGACCCATAAGCCGCTTTCTTTTGGGGATAGCGT                                                       >  SRR19973069.264409/1‑150 (MQ=23)
                                                                                              AACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATTTT‑TCTGCCCCTCCAAAACCAAAAAACCCCCGAACCGGGGTTTTACGTAAACCAGGGGAAAATGACCGATAAcccgcgttcggttgggtggagcgg                                                       >  SRR19973069.470062/1‑126 (MQ=17)
                                                                                                CTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATTGCGTCG                                                     >  SRR19973069.3524372/1‑150 (MQ=25)
                                                                                                CTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGGGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCG                                                     >  SRR19973069.3524343/1‑150 (MQ=31)
                                                                                                  GACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTG                                                   <  SRR19973069.2648885/150‑1 (MQ=32)
                                                                                                      GATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGTCAGTCATTAATTTT‑TATTCCCCACCAAAAGCAAAAACCCGCCCAACCGGGTTTTTAactaaaacaggttaaactgaaccaaaatacccgttgtttttgggaaagccaccaggtca                                               >  SRR19973069.2431070/1‑91 (MQ=0)
                                                                                                         AAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TAAGCCCCTCCAAAAGCAAAAACCCCCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACAGACCCATAAGCCGCGTTATTTTGGGGATAGCctatgttagagtc                                            >  SRR19973069.1145270/1‑137 (MQ=0)
                                                                                                         AAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAATTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTATTTTGGGTATAGCGTCGTGGACAGTC                                            >  SRR19973069.941619/1‑150 (MQ=30)
                                                                                                         AAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATTTT‑TCTGCCCCACCAAAATCAAAAAACCGCCCAACCGGGTTTTTAAGTAAACCACGTGAAACTGACCCATAAGCCGCGTTCTTTTGGGTATAGCGTAGTGGGCACTC                                            >  SRR19973069.1144382/1‑150 (MQ=19)
                                                                                                         AAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTAATTCATTTT‑TGTGCCCCTCCAAAAGCAAATACCCGCCGGAGCGGGGTTTTACGTAAACCAGGTGTAACTGTCCGATAAGCCGGTTTGTTTTGGGGATAGCGTCGTGGACAGTG                                            >  SRR19973069.941613/1‑150 (MQ=24)
                                                                                                           GCCGCTTTCTTTTGGGTTTAGTCTCGTGGACAGACATTCATTTT‑TCTGGCCCTCCAAAATCATAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAT                                          <  SRR19973069.3696644/150‑1 (MQ=27)
                                                                                                           GCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAT                                          <  SRR19973069.3026603/150‑1 (MQ=32)
                                                                                                            CCGCTTTCTTTTGGGTATAGTGTCGTGGACCGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAACCCGGTTTTTAAGTAAACCAGCTGAAACTGACCGAAAAGCCGCTTTCTTTTGGGTATAGCGTagtggccactcaag                                         >  SRR19973069.2019515/1‑136 (MQ=55)
                                                                                                                TTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTagttgccactaaataaaa                                     >  SRR19973069.540071/1‑132 (MQ=29)
                                                                                                                TTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTaaaa                                     >  SRR19973069.3776259/1‑146 (MQ=32)
                                                                                                                TTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATC                                     <  SRR19973069.3335513/150‑1 (MQ=32)
                                                                                                                TTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGCGACGTGGACAGTCATTCATA                                     >  SRR19973069.3775542/1‑150 (MQ=6)
                                                                                                                 TTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTACAAAAGCAAAAAACCCCCGAAGCGGGTTTTTACGTAAACCACGTGAAACTGAACGATAAGCCCCGTTCTTTTGGGgatagggtcgaggaaagtcaatcaaaa                                    >  SRR19973069.3306640/1‑123 (MQ=18)
                                                                                                                  TCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTT                                   <  SRR19973069.3688323/150‑1 (MQ=32)
                                                                                                                  TCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTT                                   <  SRR19973069.512450/150‑1 (MQ=32)
                                                                                                                  TCTTTTGGGTATAGTGTCGGGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAATCAAAAACCCGCAGAAGCGGGTTTTTACGTCAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTT                                   <  SRR19973069.1823516/150‑1 (MQ=28)
                                                                                                                  TCTGTTGGGTATAGTTTCGTTGACAG‑CATTTATTTTGTCTGCCCCTCCAAAAGCAAAATCCCGCAGAAGCGGGTTTTTACGTAATCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTT                                   <  SRR19973069.1823647/150‑1 (MQ=24)
                                                                                                                   CTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTT                                  <  SRR19973069.1238038/150‑1 (MQ=7)
                                                                                                                   CTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTT                                  <  SRR19973069.1238018/150‑1 (MQ=32)
                                                                                                                     TTTGTGTATAGTGTCGTGTACAGTCATTCTTATT‑TCTGCCCCTCCAAAAGAACATACCCGTCGATGGGTTTTTTTACGTATCCCATGTGTAACTGACCGATTAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATCTTTCT                                <  SRR19973069.2148133/150‑1 (MQ=13)
                                                                                                                     TTTGGGTATAGTGTCGTGGACAGTCATTCATCTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCT                                <  SRR19973069.377927/150‑1 (MQ=32)
                                                                                                                          GTATAGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCCCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATATCGTCGTGGACAGTCATTaaaatttattcccc                           >  SRR19973069.2236276/1‑136 (MQ=31)
                                                                                                                          GTATAGTGTCGTGGACAGTCATTCATTTT‑TATGCCCCCCCAAAAGCAAAAAACCCCCCAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTATTTTTGGTATAGCGTCGTGGtcactaaataaaagttttgcccc                           >  SRR19973069.786433/1‑127 (MQ=5)
                                                                                                                              AGTGTCGTGGACAGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGCTAAGCCGCTTTCTTTTGGGTTTAGCGTCGTGGACAGgcaatcatatttattccccgcca                       >  SRR19973069.3774921/1‑127 (MQ=60)
                                                                                                                                        AGAGTCATTAATTTT‑TCTGCACCTACAAAATCAAAAACCCGCCGAAGCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAA             <  SRR19973069.2920396/150‑1 (MQ=6)
                                                                                                                                          AGTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACC           <  SRR19973069.2753987/150‑1 (MQ=32)
                                                                                                                                          AGTCACTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACATCTGAAACTGAACGATAAGACGCTTTCTTTTTGGTATTGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACC           <  SRR19973069.1553699/150‑1 (MQ=5)
                                                                                                                                           GTCATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTCTAGCGTCGTGGACAGTCAATCATCTTTGTGCCCCTGCAAAAGCAAAAACCC          >  SRR19973069.3411990/1‑150 (MQ=60)
                                                                                                                                             CATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAACCCGC        >  SRR19973069.3024239/1‑150 (MQ=31)
                                                                                                                                             CATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCGTTCTGCCCCACCAAAAGCAAAAACCCGC        >  SRR19973069.3024238/1‑150 (MQ=30)
                                                                                                                                              ATTCATTTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCC       >  SRR19973069.3190411/1‑150 (MQ=32)
                                                                                                                                                   TTTT‑TCTGCCCCTCCAAAAGCAAAATCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGC  <  SRR19973069.2287160/150‑1 (MQ=31)
                                                                                                                                                    |                                                                                                                                                     
TTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTT‑TCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGC  >  CP009273/3263261‑3263557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑