Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F361 I0 R1 1353 250.7 8230166 96.0% 7900959 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,263,409 C→T 100% intergenic (+448/+166) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,263,4090CT91.2% 266.7 / 19.2 120intergenic (+448/+166)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base C (4/6);  new base T (50/56);  total (57/63)
Fisher's exact test for biased strand distribution p-value = 7.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 123 total aligned reads displayed.

TTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGA  >  CP009273/3263261‑3263554
                                                                                                                                                    |                                                                                                                                                 
ttctttTGGGTATAGTGTCGTGCACAGTCATACAAATTTCCGCCCCTCCAAAAGTAAAAACCCGCAGAAGCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATc                                                                                                                                                   <  2:2560813/149‑1 (MQ=11)
  ctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAtttt                                                                                                                                                 <  1:2967995/149‑1 (MQ=37)
   ttttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATtttt                                                                                                                                                <  2:3066388/149‑1 (MQ=37)
     ttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCt                                                                                                                                              <  1:1393499/149‑1 (MQ=37)
        ggTATTGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCACAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGcc                                                                                                                                           <  2:1797877/149‑1 (MQ=255)
        ggTATAGTGTCGTGGAAAGTCAGTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGcc                                                                                                                                           <  2:1797857/149‑1 (MQ=255)
            taGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAAACCGCCGAAGCGGGTTTTTACGTAAAAAAAGTGAAACTGACCGATAACCCGCTTTCCTTTGGGTATAGTGTCGTGGGCAGTCATTAATTTTTCTGccccgg                                                                                                                                       >  1:1688266/1‑147 (MQ=11)
            taGTGTCGGGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTc                                                                                                                                       <  2:2586354/149‑1 (MQ=255)
             aGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTcc                                                                                                                                      <  2:324509/149‑1 (MQ=255)
             aGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTcc                                                                                                                                      <  2:1475994/149‑1 (MQ=37)
               tgtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAGAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATATTTGTGGACCTCCaa                                                                                                                                    >  1:3857891/1‑149 (MQ=11)
                gtCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTGATTAATTTTTCTGCCCCTCCcaa                                                                                                                                   >  1:2654568/1‑149 (MQ=11)
                 tCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTTTTGGGTATAGTGTCGTGGAGAGGCATTCATTGTTGTGCCGCtgcgagt                                                                                                                                  >  2:3557950/1‑143 (MQ=11)
                  cGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGTTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGTGTCGTTGACAGTCATTAATTTTTCTGCCCCTCCAAAAg                                                                                                                                 <  2:1775792/149‑1 (MQ=11)
                      gACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaa                                                                                                                             <  1:839525/149‑1 (MQ=37)
                      gACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCa                                                                                                                               >  1:272189/1‑147 (MQ=17)
                      gACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCa                                                                                                                               <  2:272189/147‑1 (MQ=17)
                           tcattcatCTTTCGGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAAccc                                                                                                                        <  2:1861836/149‑1 (MQ=255)
                               tcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgccg                                                                                                                    >  2:3063113/1‑149 (MQ=17)
                                  tCTTTCTGCCCCTCCAAAAGTAAAACCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAg                                                                                                                 <  1:724401/149‑1 (MQ=255)
                                      tCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCggg                                                                                                             <  2:1226095/149‑1 (MQ=37)
                                        tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtt                                                                                                           <  1:3157951/149‑1 (MQ=37)
                                        tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGTGTAGAGTGTCGTGGACCGTCGTTGATGTTTCGGCCGCTCCAAATGCACATTCCCTCCCACGCGGGtg                                                                                                           >  2:2972764/1‑148 (MQ=11)
                                         gacccTCCAAAAGAAAAAACCCGCCGTAGCGTGTTTTTACGTAAAAAAGGTGAAACTGACCTATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                          <  1:3392760/147‑1 (MQ=11)
                                         gCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                          <  1:1801956/149‑1 (MQ=37)
                                         gCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                          <  1:3392963/149‑1 (MQ=37)
                                            ccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                       <  1:81778/149‑1 (MQ=37)
                                             cTCCAAAAGGACAAACCCGCCGCAGCGGGTTTTTACGTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTCAc                                                                                                      <  2:3956835/149‑1 (MQ=11)
                                                  aaaGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTATGCCCCTGCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                                                                                                 >  2:129715/1‑149 (MQ=255)
                                                    aGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAcc                                                                                               <  2:3504300/149‑1 (MQ=255)
                                                      tAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg                                                                                             <  2:382401/149‑1 (MQ=255)
                                                        aaaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGTGTCGTGGGCCGTCATTGATATTTCTGGCCCTCCAAAAGCAAAAAACCGCCGGAGCGGGGTTTTAGGTAAAACAggg                                                                                           >  2:884368/1‑148 (MQ=11)
                                                             ccgccgAAGCGGGTTTTTACGTAACACAGGTGAAACTGACCGATCAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAc                                                                                      <  2:3273354/149‑1 (MQ=255)
                                                             ccgccgAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAc                                                                                      <  2:1027901/149‑1 (MQ=255)
                                                              cgccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt                                                                                     >  1:2396576/1‑149 (MQ=17)
                                                                 cgcaGCGGGTTTTTACGTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAc                                                                                  <  2:2274179/146‑1 (MQ=255)
                                                                  gacgCGGGTTATTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc                                                                                 <  2:3268452/146‑1 (MQ=255)
                                                                   aaGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCg                                                                                >  1:1650347/1‑149 (MQ=255)
                                                                    aGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGa                                                                               <  1:2102970/149‑1 (MQ=37)
                                                                      cGGCTTATTACTTACAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATa                                                                             <  2:34115/149‑1 (MQ=11)
                                                                        ggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGGCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTTAACCAGGTGAAACTGACCGATAAg                                                                           >  2:2890447/1‑149 (MQ=255)
                                                                         tttcttACGTACAACAGGTGAAACTGACCGATACGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGc                                                                          <  2:3122466/145‑1 (MQ=255)
                                                                         gTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGc                                                                          <  2:3551732/149‑1 (MQ=37)
                                                                         gTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGc                                                                          <  1:3474301/149‑1 (MQ=37)
                                                                           ttttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCCTTAATTTTTGTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACATACCGATAAGCCg                                                                        >  2:1424090/1‑149 (MQ=11)
                                                                              tACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctt                                                                     >  1:2000234/1‑149 (MQ=255)
                                                                              tACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTATTCCCCTCCAAAAGAAAAAAACCCCCGCAGCGGGTTTTTACGTAAATCAGGGGAAAATGGCCGATAAGCCgggt                                                                     >  1:3558633/1‑146 (MQ=1)
                                                                                cGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttc                                                                   >  2:3320026/1‑149 (MQ=255)
                                                                                cGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTAATTTGGGTATAGTGTCGTGGACACTCATTCATTTAACAGCCCCACCAAAACCAAAAACCCCCCGAAGCGGGTTTTTAAGTAAAACAGGTGAAACTGACCGAAAAAccccgtaa                                                                   >  2:2890179/1‑143 (MQ=11)
                                                                                 ttAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGTTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAATCAAAAACCCGCCGAAGCTGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGctttct                                                                  <  1:129715/148‑1 (MQ=14)
                                                                                   aaaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttcttt                                                                <  1:2905917/149‑1 (MQ=255)
                                                                                   aaaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTGTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCGGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTGTTTACCTAAACCCGGGGAAACTGCCCGATAAGCCGcgtgagtg                                                                >  2:2083619/1‑142 (MQ=11)
                                                                                      aCAGTTTAAACTGACCGATAAGCCGCTTTCTTTTTTTTATAGTGTCGTGGACAGTCATTAATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                             <  1:501912/149‑1 (MQ=11)
                                                                                        aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTAGGGt                                                           >  2:650635/1‑149 (MQ=255)
                                                                                        aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACACTCATTCATTTTTGTGCCCCACCAAAACCAAAAACACGCCCAATCGGGTTTTTACGTAAACCAGGTGAAACTGACCGTTTATCCGCTTGATTTTgggg                                                           >  1:2274943/1‑148 (MQ=11)
                                                                                          gtaaaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                                        >  2:2797014/4‑102 (MQ=1)
                                                                                           tcaaaCTGAGATATAAGCCGCTTACTGTTGGCTATAGTGACGTTCCCAGTCATTCATTTTTATCCCACTCCACAAGCAAAAACCCGCCGCATCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                        <  2:2014152/147‑1 (MQ=11)
                                                                                           tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                        <  1:4059491/149‑1 (MQ=25)
                                                                                            gAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAg                                                       <  1:140420/149‑1 (MQ=25)
                                                                                              aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTggggacagtc                                                     >  2:550178/1‑142 (MQ=1)
                                                                                              aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTGTGGGTATAGcg                                                     >  2:3198021/1‑149 (MQ=9)
                                                                                               aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgt                                                    <  2:1097275/149‑1 (MQ=37)
                                                                                               aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCAGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTTGGGATAGcgt                                                    >  2:1613318/1‑149 (MQ=11)
                                                                                                  gACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg                                                                                                     >  2:944130/1‑97 (MQ=18)
                                                                                                     ctacaaGCCGCGTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGa                                              <  2:2654568/145‑1 (MQ=255)
                                                                                                     cGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGa                                              <  2:2654595/149‑1 (MQ=37)
                                                                                                      gATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGCCAGTCAATCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAc                                             >  2:918537/1‑149 (MQ=255)
                                                                                                      gATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAc                                             <  1:618537/149‑1 (MQ=37)
                                                                                                       aTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGCGTAGTGGACa                                            >  2:197499/1‑149 (MQ=255)
                                                                                                       aTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATTTTTCTGCCCCTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAAATGACCGATAAGCCGCGTTCTTTAGGGTAAAGCGTAGGGGaaa                                            >  1:2010371/1‑147 (MQ=11)
                                                                                                        tAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACACTCAATAATTTTTATGCCCCTCCAAAATCAAAAAACCCCCGAAGCGGGTTTTTTCGTAAAACAGGTGAAACAGACCGCTAAGCCGCTTTCTTTTGGGGATAGCGACGTggcccg                                           >  1:1647449/1‑145 (MQ=11)
                                                                                                         aaGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGCCTCGTGGACAgg                                          >  2:1290933/1‑148 (MQ=255)
                                                                                                          aGCCGGTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtc                                         <  1:700055/149‑1 (MQ=255)
                                                                                                          aGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTATTTTGGGTATAGCGTCGTGGAAAGtc                                         >  1:3775993/1‑149 (MQ=255)
                                                                                                          aGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTAAGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCCATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtc                                         >  2:2689679/1‑149 (MQ=255)
                                                                                                          aGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtc                                         <  1:700068/149‑1 (MQ=37)
                                                                                                          aGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAACAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtc                                         <  2:2550509/149‑1 (MQ=255)
                                                                                                          aGCCGCTTTCTTTTGGGTATAGTGACGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtc                                         <  1:700144/149‑1 (MQ=12)
                                                                                                             cGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTATTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGCGACGTGGACAgacctt                                      >  2:2598563/1‑144 (MQ=11)
                                                                                                             cGCTATCTTTTGGGTATTGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcatt                                      <  2:3857891/149‑1 (MQ=255)
                                                                                                              gCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcaatc                                     >  2:1503670/1‑149 (MQ=255)
                                                                                                               ctttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                       >  2:412910/1‑82 (MQ=33)
                                                                                                                tttctttTGTGTATAGTGTCGTGGACAGTCATTCAATTTTCGGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattcat                                   <  2:940231/149‑1 (MQ=255)
                                                                                                                tttctttTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattcat                                   <  2:940937/149‑1 (MQ=255)
                                                                                                                tttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCCATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtccataat                                   >  1:972032/1‑143 (MQ=11)
                                                                                                                 ttctttTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTGGTGGACAGtcaatcata                                  >  2:1031399/1‑148 (MQ=255)
                                                                                                                    ttttGGGTATAGTGTCGTGGACAGTCATTAATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCttt                               <  1:4058014/149‑1 (MQ=255)
                                                                                                                     ttagggTTTCGTGTCGTCGACAGTCTATCTTTTTTCTGCCCCTCCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTc                              <  2:2177627/146‑1 (MQ=11)
                                                                                                                          gTATCGTGTCGCGGACAGTCATTCATTTTTCGGCCCCTCCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGccc                         <  2:1616040/149‑1 (MQ=11)
                                                                                                                          gTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGGGGGCAGTGCTTCATCGTTGTGccc                         >  1:1920985/1‑149 (MQ=11)
                                                                                                                           tataGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTATTcccc                        >  2:1019467/1‑149 (MQ=11)
                                                                                                                               gtgtCGTGGACAGTCATTCATTTTTCAGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCCATAAGCCGCGTTCTTTTGGGGATAGCGTCGTGGAAAGGCAATCAACTTTATGCCCCGCCa                    >  1:1302279/1‑149 (MQ=11)
                                                                                                                                         cAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaaaa          >  1:2399937/1‑149 (MQ=37)
                                                                                                                                           gTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTTCCCCTCCAAAAGCAAAAAcc        >  2:3115446/1‑149 (MQ=255)
                                                                                                                                           gTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAcc        >  1:2070754/1‑149 (MQ=37)
                                                                                                                                            tcattcatTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAccc       <  1:853760/149‑1 (MQ=37)
                                                                                                                                              attcatTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgc     <  1:1439795/149‑1 (MQ=37)
                                                                                                                                              attcatTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTAGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgc     >  2:519539/1‑149 (MQ=255)
                                                                                                                                              attcatTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTGTTCGGGATAGCGGCGTGGACAGGCAATCATATTACTTCCCCACCATAAGAAAAAACccga     >  2:2838569/1‑148 (MQ=11)
                                                                                                                                                 catTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGa  >  1:2826521/1‑149 (MQ=37)
                                                                                                                                                    |                                                                                                                                                 
TTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGA  >  CP009273/3263261‑3263554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG  >  CP009273/3263263‑3263558
                                                                                                                                                  |                                                                                                                                                     
CTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTT                                                                                                                                                    <  SRR19973068.2986386/150‑1 (MQ=32)
   TTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTG                                                                                                                                                 <  SRR19973068.1402383/150‑1 (MQ=32)
         ATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAAACCGCCGAAGCGGGTTTTTACGTAAAAAAAGTGAAACTGACCGATAACCCGCTTTCCTTTGGGTATAGTGTCGTGGGCAGTCATTAATTTTTCTGCCCCgg                                                                                                                                           >  SRR19973068.1699107/1‑148 (MQ=26)
            GTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAGAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATATTTGTGGACCTCCAA                                                                                                                                        >  SRR19973068.3881183/1‑150 (MQ=29)
             TGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTGATTAATTTTTCTGCCCCTCCCAA                                                                                                                                       >  SRR19973068.2671185/1‑150 (MQ=29)
             TGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAA                                                                                                                                       >  SRR19973068.2671213/1‑150 (MQ=31)
                 GTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGTGTCGTGGACAGTCATTTATTTTTATGCCCCTCCAAAAGCA                                                                                                                                   >  SRR19973068.3814270/1‑150 (MQ=29)
                  TGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTTGGTATAGTGTCGTGGACAGGGATTAATTTTTCTGCCCCTCCCAAAGCAA                                                                                                                                  >  SRR19973068.3990837/1‑150 (MQ=27)
                   GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGCCGTCATTCATTTTTCTGCCCCTCCCAAAGCAAA                                                                                                                                 >  SRR19973068.274119/1‑150 (MQ=29)
                   GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGGCATTCATTTTTCTGCCCCTCCAAAAGCAAA                                                                                                                                 >  SRR19973068.274090/1‑150 (MQ=31)
                    GACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAA                                                                                                                                <  SRR19973068.845232/150‑1 (MQ=32)
                       AGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACC                                                                                                                             <  SRR19973068.3633838/150‑1 (MQ=32)
                           attcttttttctgctcctcaaatatcaaaatgccgccgtagctgttttttacgttaaagatgttatACTGACCGATTTGCCGCTTTCTTTTGTTTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCC                                                                                                                         <  SRR19973068.3278009/84‑1 (MQ=6)
                                TCTTTCTGCCCCTCCAAAAGTAAAACCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGC                                                                                                                    <  SRR19973068.729152/150‑1 (MQ=31)
                                     CTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCGTTTGGGTATAGTGTCGTGGTCAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTT                                                                                                               >  SRR19973068.1925623/1‑150 (MQ=30)
                                      TGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTGATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTT                                                                                                              >  SRR19973068.2257664/1‑150 (MQ=31)
                                      TGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTT                                                                                                              <  SRR19973068.3177360/150‑1 (MQ=32)
                                       GCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTT                                                                                                             <  SRR19973068.1813756/150‑1 (MQ=32)
                                       GCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTT                                                                                                             <  SRR19973068.3413513/150‑1 (MQ=32)
                                       GACCCTCCAAAAGAAAAAACCCGCCGTAGCGTGTTTTTACGTAAAAAAGGTGAAACTGACCTATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTT                                                                                                             <  SRR19973068.3413310/150‑1 (MQ=18)
                                        CCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAATAGCAAAAACCCGCCGAAGCGGGTTTTT                                                                                                            >  SRR19973068.2588588/1‑150 (MQ=31)
                                        CCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCACCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTT                                                                                                            >  SRR19973068.2353901/1‑150 (MQ=31)
                                          CCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAC                                                                                                          <  SRR19973068.2175372/150‑1 (MQ=32)
                                          CCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAC                                                                                                          <  SRR19973068.82648/150‑1 (MQ=32)
                                          CCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAC                                                                                                          <  SRR19973068.3069342/150‑1 (MQ=32)
                                          CCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAC                                                                                                          <  SRR19973068.3068785/150‑1 (MQ=32)
                                            TCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGT                                                                                                        <  SRR19973068.950457/150‑1 (MQ=32)
                                              CAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGGACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGTCAGGCATTCAGTTTTCAGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGTTTGCGTAA                                                                                                      >  SRR19973068.3107549/1‑150 (MQ=25)
                                                           CCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACT                                                                                         <  SRR19973068.3913508/150‑1 (MQ=32)
                                                           CCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACT                                                                                         >  SRR19973068.2411825/1‑150 (MQ=32)
                                                           CCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACT                                                                                         <  SRR19973068.4055497/150‑1 (MQ=32)
                                                              CCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAC                                                                                      <  SRR19973068.3384970/150‑1 (MQ=32)
                                                                GAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAATTTTCTGCCCCACCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTAAGTAAAACAGGTGAAAaagaacg                                                                                    >  SRR19973068.2148433/1‑143 (MQ=19)
                                                                GAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCG                                                                                    >  SRR19973068.1661050/1‑150 (MQ=20)
                                                                  AGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGAT                                                                                  <  SRR19973068.2116685/150‑1 (MQ=32)
                                                                  AGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGAT                                                                                  <  SRR19973068.3430313/150‑1 (MQ=32)
                                                                  AGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCCCCCAAGCGGGTTGTTACGTACACCAGGTGAAACTGACCGAT                                                                                  >  SRR19973068.3616115/1‑150 (MQ=28)
                                                                  AGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTATGCCCCTCCAAAAGCAAAAAACCCCCGAACCGGGTTTTTAAGTAAACCAGGTGAAAATGAACGAT                                                                                  >  SRR19973068.3617835/1‑150 (MQ=26)
                                                                       GTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCC                                                                             <  SRR19973068.1867828/150‑1 (MQ=32)
                                                                       GTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCC                                                                             <  SRR19973068.3495372/150‑1 (MQ=32)
                                                                       GTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCC                                                                             <  SRR19973068.1473491/150‑1 (MQ=32)
                                                                        TTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTATGCCCCTACAAAAGCAAAAAAACGCCCCAGCGGGGTTTTTCGTTAAACAGGTGGAACTGACCGATAAGCCG                                                                            >  SRR19973068.3143118/1‑150 (MQ=15)
                                                                           TTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTT                                                                         >  SRR19973068.2013418/1‑150 (MQ=38)
                                                                           TTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTATTCCCCTCCAAAAGAAAAAAACCCCCGCAGCGGGTTTTTACGTAAATCAGGGGAAAATGGCCGATAAGCCGggt                                                                         >  SRR19973068.3580252/1‑147 (MQ=0)
                                                                            TACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTT                                                                        <  SRR19973068.3147519/150‑1 (MQ=39)
                                                                               TTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGTTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAATCAAAAACCCGCCGAAGCTGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTT                                                                     <  SRR19973068.130900/150‑1 (MQ=37)
                                                                                 AAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTT                                                                   <  SRR19973068.2923999/150‑1 (MQ=40)
                                                                                    ACAGTTTAAACTGACCGATAAGCCGCTTTCTTTTTTTTATAGTGTCGTGGACAGTCATTAATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGG                                                                <  SRR19973068.505416/150‑1 (MQ=18)
                                                                                    ACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGG                                                                <  SRR19973068.505357/150‑1 (MQ=34)
                                                                                     CAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACACTCATTCATTTTTGTGCCCCACCAAAACCAAAAACACGCCCAATCGGGTTTTTACGTAAACCAGGTGAAACTGACCGtttatccgcttgattttgggg                                                               >  SRR19973068.2289696/1‑129 (MQ=27)
                                                                                         TGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAG                                                           <  SRR19973068.4083904/150‑1 (MQ=25)
                                                                                          GAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGC                                                          <  SRR19973068.141667/150‑1 (MQ=25)
                                                                                                   CGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCACCAAAAGCAAAAAACCCCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAATCCGCTTTATTTTGGGTATAGCGTCGTGGAC                                                 >  SRR19973068.1280682/1‑150 (MQ=6)
                                                                                                    GATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACA                                                <  SRR19973068.622656/150‑1 (MQ=32)
                                                                                                    GATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATTTTTCTGCCCCTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAAATGACCGATAAGCCGCGTTCTTTAGGGTAAAGCGTaggggaaa                                                >  SRR19973068.2023592/1‑142 (MQ=0)
                                                                                                     ATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACACTCAATAATTTTTATGCCCCTCCAAAATCAAAAAACCCCCGAAGCGGGTTTTTTCGTAAAACAGGTGAAACAGACCGCTAAGCCGCTTTCTTTTGGGGATAGCGACGTGGcccg                                               >  SRR19973068.1658144/1‑146 (MQ=1)
                                                                                                       AAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTATTTTGGGTATAGCGTCGTGGAAAGTC                                             >  SRR19973068.3798511/1‑150 (MQ=29)
                                                                                                        AGCCGGTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCA                                            <  SRR19973068.704500/150‑1 (MQ=31)
                                                                                                        AGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCA                                            <  SRR19973068.704513/150‑1 (MQ=32)
                                                                                                        AGCCGCTTTCTTTTGGGTATAGTGCCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCA                                            <  SRR19973068.704546/150‑1 (MQ=31)
                                                                                                        AGCCGCTTTCTTTTGGGTATAGTGACGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCA                                            <  SRR19973068.704590/150‑1 (MQ=7)
                                                                                                          CCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATT                                          <  SRR19973068.3024523/150‑1 (MQ=32)
                                                                                                             CTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAT                                       <  SRR19973068.2639435/150‑1 (MQ=32)
                                                                                                             CTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCCATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCcataat                                       >  SRR19973068.978481/1‑144 (MQ=30)
                                                                                                               TTGTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGTTTTTACGTACACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCT                                     <  SRR19973068.916133/150‑1 (MQ=29)
                                                                                                                 CTTTTGGGTATAGTGTCGTGGACAGTCATTAATTTTTATTCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGGGAAAATGACCCATAAGCCGCGTTCTTTTGGGgatagactcgtgggaaaggattaaaaatt                                   >  SRR19973068.55407/1‑121 (MQ=18)
                                                                                                                  TTTTTGGTATAGTGTCGTGGACAGTCATTAATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTC                                  <  SRR19973068.4082419/150‑1 (MQ=30)
                                                                                                                  TTTTGGGTATAGTGTCGTGGACAGTCATTCAATTTACTGCCCCTCCAAAAGCAAAAACCCGCCGAACCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTC                                  <  SRR19973068.3298041/150‑1 (MQ=29)
                                                                                                                  TTTTGCGTAGTGTGTCGTGGACAGTCACTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGTTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTC                                  <  SRR19973068.1236212/150‑1 (MQ=27)
                                                                                                                     TGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATATCGTCGTGGCCAGTCATTGATCTTTATGC                               >  SRR19973068.4031949/1‑150 (MQ=57)
                                                                                                                       GGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGggggcagtgcttcatcgttgtgccc                             >  SRR19973068.1933750/1‑125 (MQ=34)
                                                                                                                       GGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTCTAGCGTCGTGGTCAGTCATacaaatttatgccc                             >  SRR19973068.2671674/1‑136 (MQ=7)
                                                                                                                        GTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGCGTCGTGGccaggcaatcaaaattctgcccc                            >  SRR19973068.1046597/1‑127 (MQ=60)
                                                                                                                        GTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAataaaagttattcccc                            >  SRR19973068.574963/1‑134 (MQ=8)
                                                                                                                          ATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAACATGACCGATAAGCCCCTTTATTTTGGGgttatggtcgtgggcagggcataatatttgttccccgc                          >  SRR19973068.1698990/1‑112 (MQ=0)
                                                                                                                           TAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTAaagggactggggaagtccagcaagagttagcgcccac                         >  SRR19973068.3742255/1‑113 (MQ=38)
                                                                                                                            AGTGTCGTGGACAGTCATTCATTTTTCAGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCCATAAGCCGCGTTCTTTTGGGGATAGCGTCGTGGAAAGGCAATCAACTTTATGCCCCGCCA                        >  SRR19973068.1310812/1‑150 (MQ=36)
                                                                                                                                TCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAG                    <  SRR19973068.2033592/150‑1 (MQ=32)
                                                                                                                                    GGACAGTCATTCATTTTTCTGCCCCTCCAAAAGTTAAATCCCGCCGTAGCGGTTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAA                <  SRR19973068.4107339/150‑1 (MQ=6)
                                                                                                                                    GGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAA                <  SRR19973068.4107350/150‑1 (MQ=32)
                                                                                                                                     GACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATCATTCTTCCCCTCCAAAAGCAAAA               >  SRR19973068.955833/1‑150 (MQ=29)
                                                                                                                                      ACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAA              >  SRR19973068.2415192/1‑150 (MQ=32)
                                                                                                                                        AGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACC            >  SRR19973068.2084178/1‑150 (MQ=32)
                                                                                                                                          TCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGTAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCG          <  SRR19973068.902263/150‑1 (MQ=31)
                                                                                                                                          TCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCG          <  SRR19973068.859566/150‑1 (MQ=32)
                                                                                                                                            ATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCC        <  SRR19973068.1449219/150‑1 (MQ=32)
                                                                                                                                            ATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTGGTGTAGAGCGTCGGGGCCAGGCATTGCTCTTTCTGCCCCGGCACAAGCAAAAACACGCC        >  SRR19973068.4025333/1‑150 (MQ=16)
                                                                                                                                            ATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACCGTCATTCATCTTTCTTCCCCTCCAAAAACAAAAAACCGCC        >  SRR19973068.231234/1‑150 (MQ=60)
                                                                                                                                            ATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGGGGCCAGTCCATCATCATTCAGCCCCTCCAAAACCAAAAACCCGCC        >  SRR19973068.2460400/1‑150 (MQ=60)
                                                                                                                                              TCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGA      >  SRR19973068.2844115/1‑150 (MQ=32)
                                                                                                                                               CATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGGATAGCGTCGTGGACAGTCCATCATCGTTCAGCCCCCCCAAAAGCAAAAACCCGCCGCA     >  SRR19973068.775074/1‑150 (MQ=26)
                                                                                                                                                  TTTTCTGCCCCTCCCAAAGCAAAAACCCGGCGACGCGTGTTTTTACGTAAAACCTGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG  <  SRR19973068.1676429/150‑1 (MQ=6)
                                                                                                                                                  |                                                                                                                                                     
CTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG  >  CP009273/3263263‑3263558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑