Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,263,409 C→T 100% intergenic (+448/+166) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,263,4090CT92.2% 364.1 / 18.4 144intergenic (+448/+166)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base C (6/5);  new base T (49/82);  total (57/87)
Fisher's exact test for biased strand distribution p-value = 3.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 150 total aligned reads displayed.

TCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGA  >  CP009273/3263262‑3263554
                                                                                                                                                   |                                                                                                                                                 
tctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAtttt                                                                                                                                                 <  2:825624/150‑1 (MQ=37)
 ctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATtttt                                                                                                                                                <  2:3643498/150‑1 (MQ=37)
 ctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATtttt                                                                                                                                                <  1:4795859/150‑1 (MQ=37)
 ctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATtttt                                                                                                                                                <  1:4302944/150‑1 (MQ=37)
   tttGGGTATAGTGTCGTGGACAGTCAATCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATttttat                                                                                                                                              >  1:424673/1‑148 (MQ=11)
    ttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTg                                                                                                                                             <  1:2925633/150‑1 (MQ=37)
    ttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTg                                                                                                                                             <  1:2925302/150‑1 (MQ=37)
        gTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGccca                                                                                                                                         <  1:4682038/150‑2 (MQ=37)
            aGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAATTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCa                                                                                                                                     <  1:447550/150‑1 (MQ=255)
             gtgtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCaa                                                                                                                                    <  2:4445670/150‑1 (MQ=37)
                   tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCaa                                                                                                                              <  1:576231/150‑1 (MQ=37)
                    ggACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCaaa                                                                                                                             <  1:2780299/150‑1 (MQ=37)
                       cAGTCATTCCTCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAAc                                                                                                                          <  2:2151109/150‑1 (MQ=255)
                       cAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCaa                                                                                                                              <  2:2209258/146‑1 (MQ=37)
                       cAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCaa                                                                                                                              >  1:2209258/1‑146 (MQ=37)
                        aGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAAcc                                                                                                                         >  2:3648751/1‑150 (MQ=37)
                          tcattcatCTTTCTGCCCCTCAAAAAGTAAAAACCCGCCGTATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACccg                                                                                                                       <  2:4928439/150‑1 (MQ=255)
                          gcagtaatCTTTCTGCCACTCCAAAATTAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACccg                                                                                                                       <  2:2321301/144‑1 (MQ=11)
                           cattcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACccgc                                                                                                                      <  1:1411137/150‑1 (MQ=37)
                            attcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                                                                                                     <  2:3151065/150‑1 (MQ=37)
                             gtcctCTTTCGGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACccgccg                                                                                                                    <  2:4325863/146‑1 (MQ=255)
                                atCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAg                                                                                                                 <  1:2967941/150‑1 (MQ=37)
                                 tCTTTCTGCCCCTCCAAAAGTTAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGc                                                                                                                <  2:3206130/150‑1 (MQ=255)
                                 tCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGc                                                                                                                <  1:4173980/150‑1 (MQ=255)
                                      cTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtt                                                                                                           <  1:3917273/150‑1 (MQ=37)
                                       tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                          <  2:1518035/150‑1 (MQ=37)
                                        gCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                                                                                                         <  2:3850237/150‑1 (MQ=37)
                                         ccccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                                        <  1:750261/150‑1 (MQ=37)
                                          cccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                       <  1:1823707/150‑1 (MQ=37)
                                          cccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                       <  2:1580010/150‑1 (MQ=37)
                                           ccTCCAAAAGTACAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                                      <  2:4862935/150‑1 (MQ=255)
                                           ccTCCAAAAGTAAAACCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                                      <  2:4862954/150‑1 (MQ=255)
                                            cTCCAAAAGTAAAAACCCGCCGTAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg                                                                                                     <  2:1834099/150‑1 (MQ=255)
                                            cTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTGATTCATTTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGCAGCGGGTTTTTaag                                                                                                     >  2:1880892/1‑148 (MQ=255)
                                              ccAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTa                                                                                                   <  1:4790409/150‑1 (MQ=37)
                                              ccAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTa                                                                                                   <  1:2356959/150‑1 (MQ=37)
                                              caaaaaGTAAAAACCCGCCGCAGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTa                                                                                                   <  1:2009534/148‑1 (MQ=255)
                                                aaaaGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAcc                                                                                                                         >  1:2982925/1‑126 (MQ=38)
                                                    gTACAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg                                                                                             <  2:1047294/150‑1 (MQ=255)
                                                    gTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg                                                                                             <  1:4017093/150‑1 (MQ=38)
                                                      aaaaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGt                                                                                           <  1:4016220/150‑1 (MQ=37)
                                                       aaaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTg                                                                                          <  1:4727818/150‑1 (MQ=37)
                                                        aaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGa                                                                                         <  2:4637663/150‑1 (MQ=17)
                                                        aaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTATGTAAACCAGGTGa                                                                                         <  1:1703259/150‑1 (MQ=255)
                                                        aaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGa                                                                                         <  2:2037143/150‑1 (MQ=37)
                                                        aaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgccg                                                                                                                    >  2:3856750/1‑123 (MQ=35)
                                                           cccGCCGCATCGGGTTTTTACGTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAc                                                                                      <  2:320715/150‑1 (MQ=255)
                                                           cccGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAc                                                                                      <  2:748061/150‑1 (MQ=37)
                                                           cccGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAc                                                                                      <  1:2108171/150‑1 (MQ=37)
                                                            ccgccgCAGCGGGTTTTTACGTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACt                                                                                     <  2:2793892/150‑1 (MQ=255)
                                                             cgccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTg                                                                                    >  2:2739106/1‑150 (MQ=37)
                                                                cgAAGCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAACAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc                                                                                 <  1:5133807/150‑1 (MQ=255)
                                                                  aaGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAATTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTGTTACGTAAACCAGGTGAAACTGACCGa                                                                               >  1:5093082/1‑150 (MQ=255)
                                                                   aGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGAt                                                                              >  1:1539900/1‑150 (MQ=17)
                                                                   aGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTGTCTTTTGGGTATAGTGTCGTGGACAGTCAGTCCTATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCCTGTGAAACTGACCGAt                                                                              >  1:3178155/1‑150 (MQ=11)
                                                                    gCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATa                                                                             >  1:2697926/1‑150 (MQ=37)
                                                                        gTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGcc                                                                         <  2:3984022/150‑1 (MQ=37)
                                                                                gTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGgtgtctt                                                                 >  2:1532380/1‑150 (MQ=255)
                                                                                 tAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttcttt                                                                >  2:1100804/1‑150 (MQ=255)
                                                                                  aaaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGggttctgtc                                                               >  2:651199/1‑147 (MQ=0)
                                                                                   aaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                              <  1:4319647/150‑1 (MQ=255)
                                                                                   aaaCAGGAGAAACTGACCGATCAGCCGCGTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGAATAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                              <  2:5128933/150‑1 (MQ=11)
                                                                                       cggTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGta                                                          <  1:2622059/149‑1 (MQ=37)
                                                                                       aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAAAGGACCGATAAGCCGCGTTCTTTTGGGta                                                          >  2:3039771/1‑150 (MQ=11)
                                                                                        cgTGAAACTGTCCGATAAGCCGCATTATTTTGGGTATAGTGACGTGGACAGTCATTCATTTTTCTGCCCCTCCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtat                                                         <  2:249160/149‑1 (MQ=11)
                                                                                         gtaaaaCTGACCGATAAGCCGCTTTCTTTTGGGTAGAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                        <  2:2842602/147‑1 (MQ=12)
                                                                                          taaaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCggg                                                                                                             >  2:4573118/3‑96 (MQ=14)
                                                                                            aaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGTTTCTTTTGGGTATAgag                                                     >  2:837316/1‑148 (MQ=19)
                                                                                            aaaCTGACCGATAAGCCGCTTTCTTGTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAGAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcg                                                     <  1:4499152/150‑1 (MQ=255)
                                                                                              aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtc                                                   <  1:116440/150‑1 (MQ=37)
                                                                                                 gACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTg                                                <  2:4490315/150‑1 (MQ=37)
                                                                                                 gACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTg                                                >  1:1987897/1‑150 (MQ=255)
                                                                                                  aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTgg                                               >  1:821033/1‑150 (MQ=37)
                                                                                                  aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTATGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTAGTgg                                               >  1:4032207/1‑150 (MQ=255)
                                                                                                   ccGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAc                                                                                                                          <  2:2931892/74‑1 (MQ=34)
                                                                                                    cGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTATGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAc                                             >  1:2353066/1‑150 (MQ=255)
                                                                                                    cGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGAGGAc                                             >  2:2991926/1‑150 (MQ=17)
                                                                                                     gATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACa                                            <  1:1578037/150‑1 (MQ=37)
                                                                                                      atcaGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAACGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAg                                           <  2:4969665/147‑1 (MQ=255)
                                                                                                        aaGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                                        <  1:3335169/87‑1 (MQ=18)
                                                                                                        aaGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTGTAGTGGAAAGAGTCGTGGCCAggc                                         >  1:60028/1‑148 (MQ=11)
                                                                                                        aaGCCGCTTTCTTTTGGGTATAGTGGCGTGGACAGTCATTCATTTGTCTGCCCCTCCCAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtc                                         <  2:2431526/150‑1 (MQ=255)
                                                                                                             gCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattca                                    >  1:2743806/1‑150 (MQ=17)
                                                                                                                 tctttTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCtt                                <  2:4286542/150‑1 (MQ=37)
                                                                                                                 tctttTGGGTATAGTGTAGTTGACAGCCCTTCATTTTTCTGCCACTCCAAAAGCAAAAACCCGCCGCAGCGGTTTATTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCtt                                <  2:4286551/150‑1 (MQ=11)
                                                                                                                    tttGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCt                             >  2:606738/1‑150 (MQ=17)
                                                                                                                      tGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCtttaagc                           >  2:2227346/1‑146 (MQ=255)
                                                                                                                      tGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCAGc                           >  2:2063105/1‑150 (MQ=255)
                                                                                                                         gTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGcccc                        <  2:4073730/150‑1 (MQ=37)
                                                                                                                          tataGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCt                       >  2:5043737/1‑150 (MQ=37)
                                                                                                                               tgtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                                                                                                     >  2:1888750/1‑51 (MQ=31)
                                                                                                                                  cGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCATTATGCCCCGCCAAAAGc               >  2:1318780/1‑150 (MQ=255)
                                                                                                                                   gTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCTTGGTCAGTAAATCATCGTTGAGCCCCTCCAAAATCa              >  2:3511246/1‑150 (MQ=11)
                                                                                                                                         aGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAAcc        >  2:1825170/1‑150 (MQ=255)
                                                                                                                                          gTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAccc       >  2:1511892/1‑150 (MQ=255)
                                                                                                                                          gTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGCGTCGAGGACAGTCATTCAACATTCTGCCCCTCCAAAAGCAAAAAccc       >  2:4229197/1‑150 (MQ=11)
                                                                                                                                              ttcatTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgccg   <  1:3856755/150‑1 (MQ=37)
                                                                                                                                              ttcatTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgccg   >  2:130742/1‑150 (MQ=37)
                                                                                                                                              ttcatTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCGTTCTGCCCCTCCAAAAGCAAAAACccgccg   >  2:582672/1‑150 (MQ=255)
                                                                                                                                               tcatTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGa  >  1:4424489/1‑150 (MQ=37)
                                                                                                                                                   |                                                                                                                                                 
TCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGA  >  CP009273/3263262‑3263554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG  >  CP009273/3263263‑3263558
                                                                                                                                                  |                                                                                                                                                     
CTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTT                                                                                                                                                    <  SRR19973063.4316617/150‑1 (MQ=32)
CTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTT                                                                                                                                                    <  SRR19973063.4316692/150‑1 (MQ=32)
CTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTT                                                                                                                                                    <  SRR19973063.3281402/150‑1 (MQ=32)
CTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTT                                                                                                                                                    <  SRR19973063.4811184/150‑1 (MQ=32)
  TTTGGGTATAGTGTCGTGGACAGTCAATCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATTTTTAT                                                                                                                                                  >  SRR19973063.425861/1‑150 (MQ=28)
   TTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTG                                                                                                                                                 <  SRR19973063.570783/150‑1 (MQ=32)
   TTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTG                                                                                                                                                 <  SRR19973063.2934961/150‑1 (MQ=32)
   TTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTG                                                                                                                                                 <  SRR19973063.2934628/150‑1 (MQ=32)
       GTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCA                                                                                                                                             <  SRR19973063.4696966/150‑1 (MQ=31)
           AGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAATTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCA                                                                                                                                         <  SRR19973063.448813/150‑1 (MQ=31)
           AGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGTGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCA                                                                                                                                         <  SRR19973063.350148/150‑1 (MQ=31)
           AGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCA                                                                                                                                         <  SRR19973063.448805/150‑1 (MQ=32)
           AGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATTTTTCTGCCCCACCA                                                                                                                                         >  SRR19973063.649661/1‑150 (MQ=29)
            GTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCAAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAA                                                                                                                                        <  SRR19973063.851578/150‑1 (MQ=31)
               tgtgtgagattctttattatatattgccctcaaaaatattAAACCCGCCGAATCGGTTTTTTACGTAAATCAGGTGATTCTGACCGATACGCCGCTTTCTTTTGGTTTTAGGTTCGTGGAAAGTCATTCATCTTTCTGCCCCTCCAAAAG                                                                                                                                     <  SRR19973063.3179654/110‑1 (MQ=0)
                CGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGC                                                                                                                                    >  SRR19973063.4642689/1‑150 (MQ=32)
                 GTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCA                                                                                                                                   >  SRR19973063.3413008/1‑150 (MQ=32)
                 GTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCA                                                                                                                                   >  SRR19973063.2890937/1‑150 (MQ=32)
                 GTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCA                                                                                                                                   <  SRR19973063.4934109/150‑1 (MQ=31)
                  TGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAA                                                                                                                                  <  SRR19973063.577927/150‑1 (MQ=32)
                   GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAA                                                                                                                                 <  SRR19973063.2789253/150‑1 (MQ=32)
                     ACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAA                                                                                                                               <  SRR19973063.503153/150‑1 (MQ=32)
                      CAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAAT                                                                                                                              >  SRR19973063.2216220/1‑150 (MQ=31)
                          CATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGC                                                                                                                          <  SRR19973063.169477/150‑1 (MQ=32)
                          CATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGC                                                                                                                          <  SRR19973063.1415415/150‑1 (MQ=32)
                           ATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCC                                                                                                                         <  SRR19973063.3920104/150‑1 (MQ=32)
                               ttattaaggccactcaacaagttcAAACCCGCCGTAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG                                                                                                                     <  SRR19973063.2725852/126‑1 (MQ=22)
                               ATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG                                                                                                                     <  SRR19973063.2725764/150‑1 (MQ=32)
                               ATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG                                                                                                                     <  SRR19973063.2977427/150‑1 (MQ=32)
                                TCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGC                                                                                                                    <  SRR19973063.4187317/150‑1 (MQ=31)
                                TCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGC                                                                                                                    <  SRR19973063.4187351/150‑1 (MQ=32)
                                 gttttacgtcttcgtgacttgatttcagtcgtgtgctcgttagttcttacAACAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG                                                                                                                   <  SRR19973063.4563580/100‑1 (MQ=0)
                                     CTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTT                                                                                                               <  SRR19973063.3929772/150‑1 (MQ=32)
                                      TGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTT                                                                                                              <  SRR19973063.4160979/150‑1 (MQ=32)
                                        cccccccacgcctaaataccctcctattcgtgaTTTTACGTAATACTGGTGAACCTGACAGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTT                                                                                                            <  SRR19973063.3345802/117‑1 (MQ=18)
                                        CCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTT                                                                                                            <  SRR19973063.752450/150‑1 (MQ=32)
                                         CCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTA                                                                                                           <  SRR19973063.1829379/150‑1 (MQ=32)
                                          CCTCCAAACGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGATTTTAC                                                                                                          <  SRR19973063.3544713/150‑1 (MQ=30)
                                          CCTCCAAAAGTAAAAACCCGCCGAAGCGTGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAC                                                                                                          <  SRR19973063.3544766/150‑1 (MQ=31)
                                             CCAAAATTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTA                                                                                                       <  SRR19973063.4686121/150‑1 (MQ=27)
                                             CCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTA                                                                                                       <  SRR19973063.4805714/150‑1 (MQ=32)
                                             CCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTA                                                                                                       <  SRR19973063.2364503/150‑1 (MQ=32)
                                             CAAAAAGTAAAAACCCGCCGCAGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTA                                                                                                       <  SRR19973063.2015809/150‑1 (MQ=30)
                                              CACAAGTAAAACCCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAA                                                                                                      <  SRR19973063.3753386/150‑1 (MQ=27)
                                               AAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATGTTTCTGCCCCTCCAAAAGCAAAAACCCGCgcggaagggcaaagggctgaa                                                                                                     >  SRR19973063.2992452/1‑129 (MQ=33)
                                                   GTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAG                                                                                                 <  SRR19973063.4029982/150‑1 (MQ=34)
                                                   GTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAG                                                                                                 <  SRR19973063.4029989/150‑1 (MQ=34)
                                                     AAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGT                                                                                               <  SRR19973063.4029108/150‑1 (MQ=32)
                                                      AAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTG                                                                                              <  SRR19973063.4742887/150‑1 (MQ=32)
                                                      AAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTG                                                                                              <  SRR19973063.4744090/150‑1 (MQ=32)
                                                       AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTATGTAAACCAGGTGA                                                                                             <  SRR19973063.1708451/150‑1 (MQ=31)
                                                       AAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGA                                                                                             <  SRR19973063.3012180/150‑1 (MQ=32)
                                                          CCCGCCGAATCGGGTTATTACGTACAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAC                                                                                          <  SRR19973063.2114700/150‑1 (MQ=26)
                                                          CCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAC                                                                                          <  SRR19973063.2114765/150‑1 (MQ=32)
                                                           CCGCCGTAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACT                                                                                         <  SRR19973063.5021904/150‑1 (MQ=30)
                                                            CGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTG                                                                                        <  SRR19973063.5145638/150‑1 (MQ=32)
                                                               CGAAGCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAACAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACC                                                                                     <  SRR19973063.5150318/150‑1 (MQ=30)
                                                                 AAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAATTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTGTTACGTAAACCAGGTGAAACTGACCGA                                                                                   >  SRR19973063.5109472/1‑150 (MQ=30)
                                                                  AGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGAT                                                                                  >  SRR19973063.1544616/1‑150 (MQ=32)
                                                                  AGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGAT                                                                                  >  SRR19973063.1544610/1‑150 (MQ=20)
                                                                  AGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTGTCTTTTGGGTATAGTGTCGTGGACAGTCAGTCCTATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCCTGTGAAACTGACCGAT                                                                                  >  SRR19973063.3188377/1‑150 (MQ=27)
                                                                   GCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATA                                                                                 >  SRR19973063.2706626/1‑150 (MQ=32)
                                                                    CGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAA                                                                                <  SRR19973063.2036573/150‑1 (MQ=32)
                                                                     GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAG                                                                               <  SRR19973063.3504761/150‑1 (MQ=20)
                                                                                TAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTT                                                                    <  SRR19973063.3107903/150‑1 (MQ=42)
                                                                                 AAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTT                                                                   >  SRR19973063.3605956/1‑150 (MQ=48)
                                                                                 AAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACGGACCGATAAGCCGCattgttgt                                                                   >  SRR19973063.3605504/1‑142 (MQ=31)
                                                                                  AACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTG                                                                  <  SRR19973063.504088/150‑1 (MQ=31)
                                                                                  AAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTG                                                                  <  SRR19973063.4333380/150‑1 (MQ=38)
                                                                                      CGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTA                                                              <  SRR19973063.2630409/150‑1 (MQ=29)
                                                                                      AGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTA                                                              <  SRR19973063.4658450/150‑1 (MQ=32)
                                                                                      AGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTA                                                              <  SRR19973063.4658445/150‑1 (MQ=32)
                                                                                       GGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTAT                                                             >  SRR19973063.307444/1‑150 (MQ=29)
                                                                                          GAAACTGACCGATAAGCCTCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCACTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCTCTTTCTTTTGGGTATAGC                                                          <  SRR19973063.3952742/150‑1 (MQ=23)
                                                                                           AAACTGACCGATAAGCCGCTTTCTTGTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAGAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCG                                                         <  SRR19973063.4513593/150‑1 (MQ=24)
                                                                                             ACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTC                                                       <  SRR19973063.116829/150‑1 (MQ=29)
                                                                                               TGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGCTGTTTACCTAAACCATGTGAAACAGACCCATAATCCGCTTTCTTTTGGGTATAGCGTCGT                                                     >  SRR19973063.2176618/1‑150 (MQ=26)
                                                                                                GACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTG                                                    >  SRR19973063.1994113/1‑150 (MQ=31)
                                                                                                 ACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGG                                                   >  SRR19973063.823406/1‑150 (MQ=32)
                                                                                                 ACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTATGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTAGTGG                                                   >  SRR19973063.4045132/1‑150 (MQ=25)
                                                                                                  CCGATAAGCCGCTTTCTGTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACacaacgaaccgggaaaatacttaaaacaggtgaaaatgaccgataagcggatcactgttcggaatagagtcgtgga                                                  >  SRR19973063.2941238/1‑74 (MQ=0)
                                                                                                   CGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTATGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAATCCGCTTTCTTTTGGGTATAGCGTCGTGGAA                                                 >  SRR19973063.2360596/1‑150 (MQ=30)
                                                                                                    GATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACA                                                <  SRR19973063.1582872/150‑1 (MQ=32)
                                                                                                     ATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGCGTCGTGGgccg                                               >  SRR19973063.4583811/1‑146 (MQ=31)
                                                                                                       AAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGGGGACAGTC                                             >  SRR19973063.60241/1‑150 (MQ=60)
                                                                                                       AAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCgttctgtagtggaaagagtcgtggccaggc                                             >  SRR19973063.60238/1‑120 (MQ=12)
                                                                                                         GCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCACCAAAAGCAAAAACCCCCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCCATAAGACGCTTTATTTTGGGTATAGCGTAGTGGACAGTCAT                                           >  SRR19973063.1859515/1‑150 (MQ=6)
                                                                                                            GCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCA                                        >  SRR19973063.2752661/1‑150 (MQ=7)
                                                                                                             CTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAT                                       <  SRR19973063.1625719/150‑1 (MQ=32)
                                                                                                             CTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATATTTCTGCCCCTCCCAAAGCAAAAACCCGCCGAAGCGGTTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAT                                       <  SRR19973063.1625819/150‑1 (MQ=30)
                                                                                                                TCTTTTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTT                                    <  SRR19973063.3853829/150‑1 (MQ=32)
                                                                                                                    TTGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTG                                <  SRR19973063.2220759/150‑1 (MQ=32)
                                                                                                                     TGGGTATAGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGC                               >  SRR19973063.1221211/1‑150 (MQ=32)
                                                                                                                      GGGTATAGTGTCGTGGACAGTCAATCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAAaagacagataacaccacttaaatacagtaaagaataatggacactaaataagagttaagca                              >  SRR19973063.4379704/1‑89 (MQ=0)
                                                                                                                            AGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCA                        <  SRR19973063.3663095/150‑1 (MQ=32)
                                                                                                                            AGTGTCGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTGTTGGGTAGAGCGTCTGGGACAGTCAatcaaaagtatggccctcca                        >  SRR19973063.622060/1‑130 (MQ=0)
                                                                                                                                gaGTGGACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAG                    <  SRR19973063.2900350/148‑1 (MQ=31)
                                                                                                                                TCGTGGACAGTCATTCAATTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAG                    <  SRR19973063.3179290/150‑1 (MQ=31)
                                                                                                                                TCGTCGACAGACATTCATCTGTCTGCCCCTCCAACAGCAATAACCCGCCGAATCGGGTTTTTTCGTATACCACCTGATTCTGATCGATAATCCTCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAG                    <  SRR19973063.3743033/150‑1 (MQ=21)
                                                                                                                                      ACAGTCATTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAA              >  SRR19973063.2506478/1‑150 (MQ=32)
                                                                                                                                             TTCATTTTTCTGCCCCTCCAAATTCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG       <  SRR19973063.3869017/150‑1 (MQ=30)
                                                                                                                                             TTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG       <  SRR19973063.3215490/150‑1 (MQ=32)
                                                                                                                                             TTCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG       <  SRR19973063.3869022/150‑1 (MQ=32)
                                                                                                                                             TTCATTTTTCTGCCCCTCAAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGCGTCGTGGACAGTAATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG       <  SRR19973063.4061501/150‑1 (MQ=28)
                                                                                                                                              TCATTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCct      >  SRR19973063.4438693/1‑148 (MQ=32)
                                                                                                                                                ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG    <  SRR19973063.2357084/150‑1 (MQ=32)
                                                                                                                                                 TTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGC   <  SRR19973063.2816732/150‑1 (MQ=32)
                                                                                                                                                  TTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCG  >  SRR19973063.1525141/1‑150 (MQ=31)
                                                                                                                                                  |                                                                                                                                                     
CTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG  >  CP009273/3263263‑3263558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑