Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,535,370 C→A 100% S535R (AGC→AGA)  feoB → fused ferrous iron transporter, protein B: GTP‑binding protein/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,535,3700CA100.0% 413.0 / ‑6.7 144S535R (AGC→AGA) feoBfused ferrous iron transporter, protein B: GTP‑binding protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base C (1/2);  major base A (97/38);  minor base T (0/6);  total (98/46)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 144 total aligned reads displayed.

TGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAG  >  CP009273/3535225‑3535512
                                                                                                                                                 |                                                                                                                                              
tGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAgg                                                                                                                                              >  2:2519524/1‑148 (MQ=255)
tGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAgg                                                                                                                                              >  1:250464/1‑148 (MQ=255)
     ttGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGaaaa                                                                                                                                         <  1:634922/148‑1 (MQ=255)
          ggTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtcgtc                                                                                                                                    >  1:2552311/1‑148 (MQ=255)
          ggTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTACTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtcgtc                                                                                                                                    <  1:790300/148‑1 (MQ=255)
           gTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtcgtcg                                                                                                                                   >  2:2650712/1‑148 (MQ=255)
            tCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGa                                                                                                                                  >  1:727828/1‑148 (MQ=255)
               aTCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATaa                                                                                                                               >  1:77852/1‑148 (MQ=255)
                tCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAAc                                                                                                                              <  2:3134896/148‑1 (MQ=255)
                  aTGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACAt                                                                                                                            <  2:957104/148‑1 (MQ=255)
                   tGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATc                                                                                                                           >  1:2559432/1‑148 (MQ=255)
                   tGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATc                                                                                                                           <  2:2525020/148‑1 (MQ=255)
                    gTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCa                                                                                                                          <  1:1059427/148‑1 (MQ=255)
                    gTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCa                                                                                                                          <  1:1059612/148‑1 (MQ=255)
                    gTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCa                                                                                                                          >  2:2064588/1‑148 (MQ=255)
                     tACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCaa                                                                                                                         <  1:2862546/148‑1 (MQ=255)
                      aCCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAAc                                                                                                                        >  2:2478544/1‑148 (MQ=255)
                        cacaCGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGa                                                                                                                      >  1:2377037/1‑148 (MQ=255)
                        cacaCGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGa                                                                                                                      <  2:3164833/148‑1 (MQ=255)
                        cacaCGTTAAAAGCCTGATTATCCACACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGa                                                                                                                      <  1:1934739/148‑1 (MQ=255)
                         acacGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGAc                                                                                                                     >  2:3267693/1‑148 (MQ=255)
                          cacGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACt                                                                                                                    >  1:1861049/1‑148 (MQ=255)
                               tAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGgcg                                                                                                               >  2:112896/1‑148 (MQ=255)
                               tAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGgcg                                                                                                               >  1:215386/1‑148 (MQ=255)
                                  aaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTg                                                                                                            >  1:1164421/1‑148 (MQ=255)
                                  aaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTg                                                                                                            >  2:1388522/1‑148 (MQ=255)
                                   aGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTgg                                                                                                           >  1:1698695/1‑148 (MQ=255)
                                   aGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTgg                                                                                                           >  1:1698748/1‑148 (MQ=255)
                                     ccTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcg                                                                                                         >  2:117968/1‑148 (MQ=255)
                                      cTGATGATCCTGACCTGGCAGGGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACAACAACGACTCGGCGCTGGcgt                                                                                                        <  2:832093/148‑1 (MQ=255)
                                         aTTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGTTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccg                                                                                                     >  2:1542827/1‑148 (MQ=255)
                                         aTTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACCTCAACGACTCGGCGCTGGcgtccg                                                                                                     >  2:1806593/1‑148 (MQ=255)
                                             tCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAg                                                                                                 >  1:2482868/1‑148 (MQ=255)
                                             tCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAg                                                                                                 >  1:3134008/1‑148 (MQ=255)
                                               cAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGcc                                                                                               >  2:344602/1‑148 (MQ=255)
                                               cAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGcc                                                                                               >  2:343689/1‑148 (MQ=255)
                                                aGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCg                                                                                              >  2:3509249/1‑148 (MQ=255)
                                                aGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCg                                                                                              >  2:1751183/1‑148 (MQ=255)
                                                aGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCg                                                                                              >  2:1621125/1‑148 (MQ=255)
                                                 gACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCgg                                                                                             >  1:3189423/1‑148 (MQ=255)
                                                       gCAGCGTCTTAAAGGCTTCGTTCTGCGTGCTGTTAAAGTGATCATCAACGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATc                                                                                       <  2:1428542/148‑1 (MQ=255)
                                                          gCGTCTTAATGGCTTGGTTCTGGGTGCTGGTAATGTTATCAACACCGTCAGAATTTTCCGGAGCGCTTTAAACAGCTTCAGGCTGAGAGGTAAAATCGTCGATAACACCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCAcc                                                                                    <  1:1853858/148‑1 (MQ=255)
                                                          gCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAACAACATCGACAGCATTTTCCGGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCAcc                                                                                    <  2:52953/148‑1 (MQ=255)
                                                           cGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCAccc                                                                                   >  1:2597722/1‑148 (MQ=255)
                                                              cTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCgg                                                                                >  2:2318676/1‑148 (MQ=255)
                                                              cTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCgg                                                                                >  2:530840/1‑148 (MQ=255)
                                                              cTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCgg                                                                                >  1:1691718/1‑148 (MQ=255)
                                                                   aGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTc                                                                           >  1:1671684/1‑148 (MQ=255)
                                                                    ggCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCa                                                                          <  2:255104/148‑1 (MQ=255)
                                                                         cGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCa                                                                     >  2:1956668/1‑148 (MQ=255)
                                                                         cGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCa                                                                     >  2:1956491/1‑148 (MQ=255)
                                                                            tCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAAtt                                                                  >  2:1181311/1‑148 (MQ=255)
                                                                             cTGCTTGCTGTTAAATTTATAATCATCGTCAGAATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCTATAAAATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTg                                                                 <  2:1062190/148‑1 (MQ=255)
                                                                             cTGCGTGCTGTTAAAGTGATACTCATCGTCAGAATTTTCCTGAGCGATTTCAACAGCTTCTCGCTGATAGGTAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTg                                                                 <  2:1062209/148‑1 (MQ=255)
                                                                              tgggtggtgTTAATGTTATAATAATCGTCAGAATTTTCCTTCGCGATTTAAACAGTTTCTCGCTGAGAGGTAAAATCGTCTATAACACCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTgg                                                                <  1:1751183/145‑1 (MQ=255)
                                                                              ggggTGCTGGTAAAGTGATCATCATCGTCAGATTTTTCCTGAGCGTTTTAAACTGCTTCTCGCTGAGTGGTAAATTCGTCTATAACAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTgg                                                                <  1:3509249/145‑1 (MQ=255)
                                                                               gCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGc                                                               >  1:1967781/1‑148 (MQ=255)
                                                                               gCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGc                                                               >  2:3112466/1‑148 (MQ=255)
                                                                               gCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGc                                                               >  1:3042641/1‑148 (MQ=255)
                                                                               gCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGAGCACCCCGGTCTTCAAGCCAATTGGc                                                               >  1:1967779/1‑148 (MQ=255)
                                                                                   gCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGc                                                           >  1:25163/1‑148 (MQ=255)
                                                                                     tGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCAt                                                         >  1:3369206/1‑148 (MQ=255)
                                                                                      ggTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATg                                                        >  1:3273254/1‑148 (MQ=255)
                                                                                       gTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGa                                                       >  2:1498342/1‑148 (MQ=255)
                                                                                        tAATGTTATCATAATCGTCAGATTTTTCCTGAGCGCTTACACCAGCTTCGGGGTTTTTGGGAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGaa                                                      <  1:2519524/148‑1 (MQ=255)
                                                                                         aaaGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAg                                                     >  2:2407956/1‑148 (MQ=255)
                                                                                         aaaGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAg                                                     >  2:2408536/1‑148 (MQ=255)
                                                                                         aaaGTGATCATAATCTTCTGATTTTTCCTGCGCGCTGTAACCAGCTTCTCGGTGAGTGTCAAATTGGTCTATAAAACCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAg                                                     <  1:3404202/148‑1 (MQ=255)
                                                                                          aaGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGa                                                    >  1:3183886/1‑148 (MQ=255)
                                                                                            gTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATa                                                  <  2:2912406/148‑1 (MQ=255)
                                                                                               atcatcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAAGTGGCGTGCATGAAGATAACt                                               >  2:298757/1‑148 (MQ=255)
                                                                                                 catcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTgg                                             <  2:1796413/148‑1 (MQ=255)
                                                                                                 catcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTgg                                             >  1:62281/1‑148 (MQ=255)
                                                                                                   gcctggTCAGCATTTTCCGGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggca                                           <  2:1932588/143‑1 (MQ=255)
                                                                                                     atcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggcagg                                         <  2:77852/148‑1 (MQ=255)
                                                                                                       cGTCAGCATTTTCCTGAGCGCTTTCAACCGCTTCTCGCTGAGAGGTAAAATCGTCGATAACAACCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggcaggca                                       <  1:1760357/148‑1 (MQ=255)
                                                                                                         tCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAAc                                     >  1:73208/1‑148 (MQ=255)
                                                                                                          cAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACg                                    >  1:3231038/1‑148 (MQ=255)
                                                                                                           aGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACgg                                   >  1:2345300/1‑148 (MQ=255)
                                                                                                            gCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGt                                  >  2:2988819/1‑148 (MQ=255)
                                                                                                                tttCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGTCGTGCATGAAGATAACTGGCAGGCAACGGTTGGc                              >  2:2677872/1‑148 (MQ=255)
                                                                                                                   ccTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAGTTGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTg                           >  2:1117280/1‑148 (MQ=255)
                                                                                                                      gAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGttt                        >  1:78214/1‑148 (MQ=255)
                                                                                                                        gcgcTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTAc                      >  2:2528365/1‑148 (MQ=255)
                                                                                                                            tttCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTAGAGGt                  >  1:1047492/1‑148 (MQ=255)
                                                                                                                            tttCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGt                  >  1:26633/1‑148 (MQ=255)
                                                                                                                            tttCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGt                  >  1:1047458/1‑148 (MQ=255)
                                                                                                                             ttCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTg                 >  1:2889204/1‑148 (MQ=255)
                                                                                                                             ttCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTg                 >  2:3265394/1‑148 (MQ=255)
                                                                                                                             ttCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTg                 >  2:3262426/1‑148 (MQ=255)
                                                                                                                              tCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGc                <  2:2242047/148‑1 (MQ=255)
                                                                                                                              acAACCGCTTCCCGCTGAGAGGCAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGc                <  2:1256783/147‑1 (MQ=255)
                                                                                                                               cAACAGCTTCTCGCTGAGCGGTAAAATCGTCTATAACACCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGcc               <  1:1099696/148‑1 (MQ=255)
                                                                                                                               aaaCAGCTTCTCTGTGTGTGGTAAAATCTACGATAACACCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGcc               <  1:1099728/147‑1 (MQ=255)
                                                                                                                                 aCAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTCGGCCTGTTTACAGGTGCCAt             >  2:2095361/1‑148 (MQ=255)
                                                                                                                                  cAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATg            >  1:1262544/1‑148 (MQ=255)
                                                                                                                                  cAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATg            <  1:1181311/148‑1 (MQ=255)
                                                                                                                                  cAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATg            <  2:855146/148‑1 (MQ=255)
                                                                                                                                     cTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCg         >  1:1014437/1‑148 (MQ=255)
                                                                                                                                       tctcGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaa       >  1:2910237/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAG  >  CP009273/3535225‑3535512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTA  >  CP009273/3535221‑3535517
                                                                                                                                                     |                                                                                                                                                   
GTCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGA                                                                                                                                                     >  SRR19973067.860908/1‑150 (MQ=60)
GTCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGA                                                                                                                                                     >  SRR19973067.860853/1‑150 (MQ=60)
  CATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGG                                                                                                                                                   >  SRR19973067.252031/1‑150 (MQ=60)
  CATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGG                                                                                                                                                   >  SRR19973067.252195/1‑150 (MQ=60)
  CATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGG                                                                                                                                                   <  SRR19973067.1245117/150‑1 (MQ=60)
         TTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATC                                                                                                                                            <  SRR19973067.639182/150‑1 (MQ=60)
            CCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTC                                                                                                                                         >  SRR19973067.2569003/1‑150 (MQ=60)
              GGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGA                                                                                                                                       >  SRR19973067.732885/1‑150 (MQ=60)
              GGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTACTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGA                                                                                                                                       <  SRR19973067.795765/150‑1 (MQ=60)
                 CTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAA                                                                                                                                    >  SRR19973067.78727/1‑150 (MQ=60)
                  TATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAAC                                                                                                                                   >  SRR19973067.2035135/1‑150 (MQ=60)
                    TCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACAT                                                                                                                                 <  SRR19973067.672330/150‑1 (MQ=60)
                     CATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATC                                                                                                                                >  SRR19973067.2576160/1‑150 (MQ=60)
                        GTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAAC                                                                                                                             <  SRR19973067.1066875/150‑1 (MQ=60)
                        GTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAAC                                                                                                                             <  SRR19973067.1064336/150‑1 (MQ=60)
                        GTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAAC                                                                                                                             <  SRR19973067.1066689/150‑1 (MQ=60)
                         TACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACG                                                                                                                            <  SRR19973067.2880892/150‑1 (MQ=60)
                          ACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGA                                                                                                                           >  SRR19973067.3195295/1‑150 (MQ=60)
                          ACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGA                                                                                                                           >  SRR19973067.2392483/1‑150 (MQ=60)
                            CACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACT                                                                                                                         >  SRR19973067.1873733/1‑150 (MQ=60)
                            CACACGTTAAAAGCCTGATTATCCACACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACT                                                                                                                         <  SRR19973067.1947937/150‑1 (MQ=60)
                                 GTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCG                                                                                                                    >  SRR19973067.216979/1‑150 (MQ=60)
                                    AAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTG                                                                                                                 >  SRR19973067.1172163/1‑150 (MQ=60)
                                     AAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGG                                                                                                                >  SRR19973067.1710374/1‑150 (MQ=60)
                                     AAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGG                                                                                                                >  SRR19973067.1710428/1‑150 (MQ=60)
                                     AAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGG                                                                                                                >  SRR19973067.1711228/1‑150 (MQ=60)
                                        GCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGT                                                                                                             >  SRR19973067.1072863/1‑150 (MQ=60)
                                           TGTTTAACCAGACCTGGCAGCGTCTGAACGGCTTCTTTCGGCGTGCTGTTAACGTTATCAACATCGACAGATTTTGCCTGAGCGCTTCCAACCGTTTCGCGCTGAGAGGCAAAAGCGTCGATAAAACCCACGACTCGGCGCTGGCGTCCG                                                                                                          <  SRR19973067.1183404/150‑1 (MQ=21)
                                           TGATTATCCAGACGTGGCAGCGTCTTAAAGGCTTCGTTCTGCGTGCTGTTAACGTGATCACCATCGTCAGCATTTTCCTGAGCGATTACAACCGCTTCTCGCTGCGAGGTAAAATCGTCTATAACAACCACGACTCGGCGCTGGCGTCCG                                                                                                          <  SRR19973067.1183166/150‑1 (MQ=60)
                                           TGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCG                                                                                                          >  SRR19973067.1705045/1‑150 (MQ=60)
                                               TATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAG                                                                                                      >  SRR19973067.3153918/1‑150 (MQ=60)
                                               TATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAG                                                                                                      >  SRR19973067.2499193/1‑150 (MQ=60)
                                                ATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGC                                                                                                     >  SRR19973067.1808493/1‑150 (MQ=60)
                                                   CAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGG                                                                                                  >  SRR19973067.3209576/1‑150 (MQ=60)
                                                             AGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGTTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCC                                                                                        >  SRR19973067.297946/1‑150 (MQ=60)
                                                             AGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCC                                                                                        >  SRR19973067.2614618/1‑150 (MQ=60)
                                                              GCTTCTGAATGGCTTGGTTCTGGGTGCTGTTAATGTCATCATCATGGTCAGATTTTTCGTGAGCGCTTACAACCGCTTCCGGCTGAGCGGCAAAATCTTCTAAAACAACCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCC                                                                                       <  SRR19973067.1866989/150‑1 (MQ=33)
                                                              GCGTCTTAATGGCTTGGTTCTGGGTGCTGGTAATGTTATCAACACCGTCAGAATTTTCCGGAGCGCTTTAAACAGCTTCAGGCTGAGAGGTAAAATCGTCGATAACACCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCC                                                                                       <  SRR19973067.1866493/150‑1 (MQ=60)
                                                                GTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGG                                                                                     >  SRR19973067.1703365/1‑150 (MQ=60)
                                                                    taagggcttcgttcggcgtgctgttaatgttataataatcgtcctatttgtcgtgagggcttaCAACAGCTTCTGGCTGAGTGCTAAAAGCGTCTATAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTT                                                                                 <  SRR19973067.1019795/87‑1 (MQ=60)
                                                                     AAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTC                                                                                >  SRR19973067.1683213/1‑150 (MQ=60)
                                                                        gggttgttgcggggtggtgtTAAAGTTATCATCACCGTCAGATTTTTCCGGAGCGCTTACAACCGCTTCTCGCTGAGAGGTAAAATCGTCTATAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAG                                                                             <  SRR19973067.3410135/130‑1 (MQ=60)
                                                                                 CTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGC                                                                    >  SRR19973067.3062023/1‑150 (MQ=60)
                                                                                 CTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGC                                                                    >  SRR19973067.1981136/1‑150 (MQ=60)
                                                                                 CTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGAGCACCCCGGTCTTCAAGCCAATTGGC                                                                    >  SRR19973067.1981134/1‑150 (MQ=60)
                                                                                  tgggtggtgttaatgttataataATCGTCAGAATTTTCCTTCGCGATTTAAACAGTTTCTCGCTGAGAGGTAAAATCGTCTATAACACCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCG                                                                   <  SRR19973067.1763120/127‑1 (MQ=60)
                                                                                  gggGTGCTGGTAAAGTGATCATCATCGTCAGATTTTTCCTGAGCGTTTTAAACTGCTTCTCGCTGAGTGGTAAATTCGTCTATAACAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCG                                                                   <  SRR19973067.3531473/147‑1 (MQ=60)
                                                                                     GTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGC                                                                >  SRR19973067.25551/1‑150 (MQ=60)
                                                                                     GTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGC                                                                >  SRR19973067.77578/1‑150 (MQ=60)
                                                                                       GCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCAT                                                              >  SRR19973067.3390606/1‑150 (MQ=60)
                                                                                        CTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATG                                                             >  SRR19973067.3293730/1‑150 (MQ=60)
                                                                                        CTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATG                                                             >  SRR19973067.3293729/1‑150 (MQ=60)
                                                                                            TAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGTCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGA                                                         >  SRR19973067.2381901/1‑150 (MQ=60)
                                                                                            TAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGA                                                         >  SRR19973067.3204023/1‑150 (MQ=60)
                                                                                             aaagttatcaaattcttcagattttgcgtgtgcgatttaAACAGATTCTCGTTGAGAGGTAAAATCGTCTATAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGAT                                                        <  SRR19973067.1654894/111‑1 (MQ=60)
                                                                                              AAGTGATAAGCATCGTCATATTTTTCCTGAGCGCTTTAAACAGCTTCTCGCTGAGTGGGAAAATCGTCTATAACAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATA                                                       <  SRR19973067.2695239/150‑1 (MQ=60)
                                                                                                   ATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGG                                                  >  SRR19973067.63034/1‑150 (MQ=60)
                                                                                                           CGTCAGCATTTTCCTGAGCGCTTTCAACCGCTTCTCGCTGAGAGGTAAAATCGTCGATAACAACCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAAC                                          <  SRR19973067.1772341/150‑1 (MQ=60)
                                                                                                           CGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAAC                                          >  SRR19973067.74047/1‑150 (MQ=60)
                                                                                                            GTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACG                                         >  SRR19973067.3251315/1‑150 (MQ=60)
                                                                                                             TCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGG                                        >  SRR19973067.2360630/1‑150 (MQ=60)
                                                                                                                      TCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGT                               <  SRR19973067.3250265/150‑1 (MQ=60)
                                                                                                                        CTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTT                             >  SRR19973067.79089/1‑150 (MQ=60)
                                                                                                                        CTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTT                             >  SRR19973067.79738/1‑150 (MQ=60)
                                                                                                                        CTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTT                             >  SRR19973067.1970261/1‑150 (MQ=60)
                                                                                                                        CTGAGCGCTTCCAACAGCTTCTCGCTGTGTGGGAAAATCGTCTATACCAACCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTT                             <  SRR19973067.2766544/150‑1 (MQ=60)
                                                                                                                             CGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGG                        >  SRR19973067.548917/1‑150 (MQ=60)
                                                                                                                              GCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTAGAGGT                       >  SRR19973067.1054685/1‑150 (MQ=60)
                                                                                                                              GCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGT                       >  SRR19973067.27050/1‑150 (MQ=60)
                                                                                                                              GCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGT                       >  SRR19973067.1054651/1‑150 (MQ=60)
                                                                                                                               CTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTG                      >  SRR19973067.2907671/1‑150 (MQ=60)
                                                                                                                                   CAACAGCTTCTCGCTTAGTGGGAAAATCGTCTATAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCAT                  <  SRR19973067.2585783/150‑1 (MQ=60)
                                                                                                                                   CAACAGCTTCTCGCTGAGCGGTAAAATCGTCTATAACACCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCAT                  <  SRR19973067.1107177/150‑1 (MQ=60)
                                                                                                                                    AACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATG                 >  SRR19973067.1271107/1‑150 (MQ=60)
                                                                                                                                      CAGCTTCTCGCTGCGTGGTAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGC               <  SRR19973067.1189110/150‑1 (MQ=60)
                                                                                                                                       AGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCG              >  SRR19973067.1021400/1‑150 (MQ=60)
                                                                                                                                       AGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCG              >  SRR19973067.1158452/1‑150 (MQ=60)
                                                                                                                                        GCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGA             >  SRR19973067.2567147/1‑150 (MQ=60)
                                                                                                                                         CTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAA            >  SRR19973067.2928778/1‑150 (MQ=60)
                                                                                                                                                   AGTGGTAAAATCGTCTATAACAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTA  <  SRR19973067.2858705/150‑1 (MQ=60)
                                                                                                                                                   AGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTA  >  SRR19973067.1607133/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
GTCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTA  >  CP009273/3535221‑3535517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑