Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F434 I0 R1	1130	215.9	7031662	97.1%	6827743	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,535,370	C→A	100%	S535R (AGC→AGA)	feoB →	fused ferrous iron transporter, protein B: GTP‑binding protein/membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,535,370	0	C	A	97.5%	508.1 / ‑4.7	181	S535R (AGC→AGA)	feoB	fused ferrous iron transporter, protein B: GTP‑binding protein/membrane protein
Reads supporting (aligned to +/- strand): ref base C (0/2); major base A (107/61); minor base T (0/11); total (107/74)
Fisher's exact test for biased strand distribution p-value = 2.73e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 182 total aligned reads displayed.

TGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTA  >  CP009273/3535225‑3535517

tGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAgg                                                                                                                                                   <  1:2743084/148‑1 (MQ=255)

tGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAgg                                                                                                                                                   <  1:2326235/148‑1 (MQ=255)

 ggAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAggg                                                                                                                                                  >  2:943729/1‑148 (MQ=255)

 ggAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAggg                                                                                                                                                  >  1:383481/1‑148 (MQ=255)

 ggAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAggg                                                                                                                                                  <  1:1632495/148‑1 (MQ=255)

  gAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGa                                                                                                                                                 >  1:1023803/1‑148 (MQ=255)

  gAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGa                                                                                                                                                 >  1:3077934/1‑148 (MQ=255)

  gAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGa                                                                                                                                                 <  1:2727411/148‑1 (MQ=255)

  gAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGa                                                                                                                                                 >  1:1023742/1‑148 (MQ=255)

    gTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGaaa                                                                                                                                               <  1:1594077/148‑1 (MQ=255)

    gTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGaaa                                                                                                                                               <  1:2186475/148‑1 (MQ=255)

       gCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtc                                                                                                                                            <  2:1660827/148‑1 (MQ=255)

        ccGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtcg                                                                                                                                           <  1:2792810/148‑1 (MQ=255)

        ccGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtcg                                                                                                                                           <  2:1498310/148‑1 (MQ=255)

        ccGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtcg                                                                                                                                           <  2:1498392/148‑1 (MQ=255)

            tCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGa                                                                                                                                       <  1:3360644/148‑1 (MQ=255)

              tATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATa                                                                                                                                     >  2:348742/1‑148 (MQ=255)

              tATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATa                                                                                                                                     <  2:2088070/148‑1 (MQ=255)

                 cATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACa                                                                                                                                  >  2:2971997/1‑148 (MQ=255)

                   tGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATc                                                                                                                                >  1:99589/1‑148 (MQ=255)

                   tGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATc                                                                                                                                >  2:1306353/1‑148 (MQ=255)

                    gTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCa                                                                                                                               >  1:1272440/1‑148 (MQ=255)

                    gTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCa                                                                                                                               >  1:1477236/1‑148 (MQ=255)

                    gTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCa                                                                                                                               >  1:1477237/1‑148 (MQ=255)

                    gTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCa                                                                                                                               <  1:2643452/148‑1 (MQ=255)

                        cacaCGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGa                                                                                                                           <  2:801981/148‑1 (MQ=255)

                         acacGTTAAATGCCTTATTATCCAGACGTGACAGCGTCTTAATGGCTTCTTTCTGGGTGGTGTTAATGTGATAATAATGGTCAGATTTTTCGTGAGCGCTTTAAACTGCTTCTCGCTGTGAGGTAAAATCGTCGATAACATCAACGAc                                                                                                                          <  2:1628002/148‑1 (MQ=255)

                          cacGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACt                                                                                                                         >  2:1681766/1‑148 (MQ=255)

                           acGTTAAAGGCCTTATTATCCAGACCGGGCTGCGTCTGAAAGGATTCGTTCTGCGTGCTGGTAATGTGATATTCATCGTCAGATTTTTCCTGCGCGCTTTAAAATGATTATCGCTGTGTGGGAAAATCGTCTTTAACATCAACGACTc                                                                                                                        <  2:275801/148‑1 (MQ=255)

                               tAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGgcg                                                                                                                    >  2:2124302/1‑148 (MQ=255)

                                aaaaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGgcgc                                                                                                                   >  1:256827/1‑148 (MQ=255)

                                  aaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTg                                                                                                                 >  1:3444736/1‑148 (MQ=255)

                                     ccTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcg                                                                                                              >  2:2614054/1‑148 (MQ=255)

                                        gATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtcc                                                                                                           >  1:1940311/1‑148 (MQ=255)

                                        gATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtcc                                                                                                           >  1:1939180/1‑148 (MQ=255)

                                        gATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTATCCTGAGCGCTTTCAACAGCTTCTCGATGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtcc                                                                                                           >  1:379130/1‑148 (MQ=255)

                                          ttATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccgt                                                                                                         >  2:2503321/1‑148 (MQ=255)

                                             tCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAg                                                                                                      >  2:3363416/1‑148 (MQ=255)

                                             tCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGAGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAg                                                                                                      >  2:2783088/1‑148 (MQ=255)

                                                 gACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCgg                                                                                                  >  1:162604/1‑148 (MQ=255)

                                                  aCCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCggg                                                                                                 >  1:2211160/1‑148 (MQ=255)

                                                   ccTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTAAACCGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGt                                                                                                <  2:266933/148‑1 (MQ=255)

                                                        cAGGGTCTTAATGGCTTGGTTCTGCGTGGTGTTAATGTGATCATCATCGCCCGCTTTTTCCTGAGCGCTTTCAACCGCTTCTCGCTGAGAGGTAAATTCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCa                                                                                           <  1:2807207/148‑1 (MQ=255)

                                                          gCGTCTGAACGGTTTGGTTCTGCGTGCTGGTAAAGTGATCATCCTCGTCAGAATTTTCCGGCGCGCTTTAAACAGCTTCTCGCTGTGAGGTAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCAcc                                                                                         <  1:2044124/148‑1 (MQ=255)

                                                           cGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCAccc                                                                                        >  1:1213987/1‑148 (MQ=255)

                                                              cTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCgg                                                                                     >  1:173088/1‑148 (MQ=255)

                                                                 aaaGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCt                                                                                  <  2:2012346/148‑1 (MQ=255)

                                                                 aaaGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCt                                                                                  >  2:3255747/1‑148 (MQ=255)

                                                                 aaaGGCTTCGTTCTCCGTGCTGGTAAAGTGATCATCAGCGTCTGCATTTTCCGGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCt                                                                                  <  2:339598/148‑1 (MQ=255)

                                                                  aaGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCtt                                                                                 >  1:654937/1‑148 (MQ=255)

                                                                    ggCTGCGGTCGGCGTGCTGGTAACGTGATCATCCTCGGCAGCATTTTCCTGCGCGCTTTCAACCGCTTCTCGCTGAGTGGTAAAATCGTCGATAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCa                                                                               <  2:2374650/148‑1 (MQ=255)

                                                                      tttgtttCTGGGTGGTTTTAATGTTATAATCATCGTCATCTTTTTCCTGCGCGATTACAACAGCTTCTCGCTGAGTGGTAAAATCGTCTATAACAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAg                                                                             <  1:3411669/143‑1 (MQ=255)

                                                                      cTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAg                                                                             >  1:2842899/1‑148 (MQ=255)

                                                                        tCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGcc                                                                           <  2:1343086/148‑1 (MQ=255)

                                                                        tCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCATCAAGcc                                                                           >  2:428806/1‑148 (MQ=255)

                                                                        tCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGATTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGcc                                                                           <  2:3322066/148‑1 (MQ=255)

                                                                         ctttCTGCGTGCTGTTAATGTTATCATCATGGTCAGATTTTTCCTGAGCGCTTACACCAGCTTCTCGCGGAGTGGTAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCa                                                                          <  1:858358/146‑1 (MQ=255)

                                                                           ttCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAAt                                                                        >  1:2503530/1‑148 (MQ=255)

                                                                            tCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAAtt                                                                       >  2:64197/1‑148 (MQ=255)

                                                                                   gCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGc                                                                >  2:1710966/1‑148 (MQ=255)

                                                                                   gCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGc                                                                >  1:1684829/1‑148 (MQ=255)

                                                                                   gCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGc                                                                >  1:1685146/1‑148 (MQ=255)

                                                                                     tGGTATAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTCCAACAGGTTCTCGCTTAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCAt                                                              <  2:231172/148‑1 (MQ=255)

                                                                                      ggTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATg                                                             >  1:1868759/1‑148 (MQ=255)

                                                                                        tAAAGTGATCATCATCGTCAGAATTTTCCGGAGCGCTTTCAACAGCTTCTCGCTGCGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGaa                                                           <  2:1473781/148‑1 (MQ=255)

                                                                                         accttGATCATCATCGTAAGCATTTTCCTGAGCGATTGAACAAGCTTCTCGCTGAGAGGTAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAg                                                          <  2:2853232/144‑1 (MQ=255)

                                                                                          aaGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCAGGAAGa                                                         >  1:437782/1‑148 (MQ=255)

                                                                                           aGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGAt                                                        >  1:2480261/1‑148 (MQ=255)

                                                                                              gATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAAc                                                     >  2:2591704/1‑148 (MQ=255)

                                                                                                tcatcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTg                                                   >  1:2614482/1‑148 (MQ=255)

                                                                                                  atcatgTTCAGATTTTTCCTGCGGGTTTTCAACTGGTTCTCGCTTTGTGGTAAAATCGTCTATAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggc                                                 <  1:2432447/148‑1 (MQ=255)

                                                                                                  atcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggc                                                 <  2:3021569/148‑1 (MQ=255)

                                                                                                  atcagcGTCAGATTTTTCCTGAGCGCTTTCAACTGCTTCTCGCTGAGTGTAAAAATCGTCGAAAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggc                                                 <  1:2436996/148‑1 (MQ=255)

                                                                                                     atcGTCCGCATTTTCGGGTGCGATTCCAACCGCTTCTGGGTTAGTGGTAAAAGCGTCTTAAACACCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggcagg                                              <  1:2989449/148‑1 (MQ=255)

                                                                                                         tCAGCATTTTCCTGCGCGCTTTCAACAGCTTCTCGCTGAGAGGCAAAATCGTCGATAACAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAAc                                          <  2:1560899/148‑1 (MQ=255)

                                                                                                         tCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCCATTGGCGTGCATGAAGATAACTGGCAGGCAAc                                          >  2:3357249/1‑148 (MQ=255)

                                                                                                         tCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAAc                                          <  2:890278/148‑1 (MQ=255)

                                                                                                         tCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAAc                                          >  2:2370295/1‑148 (MQ=255)

                                                                                                          cAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACg                                         >  2:186078/1‑148 (MQ=255)

                                                                                                          cAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACg                                         >  2:3404416/1‑148 (MQ=255)

                                                                                                            gctttttCCTGAGCGCTTTCAACAGCTTCTCGCTGAGTGGGAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGt                                       <  1:1379610/145‑1 (MQ=255)

                                                                                                            gctttgtCCTGTGCGCTTTCAACAGCTTCTCGCTGTGAGGGAAAATGGTCTATAACACCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGt                                       <  1:3124169/142‑1 (MQ=255)

                                                                                                                tttCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGc                                   >  2:793877/1‑148 (MQ=255)

                                                                                                                   ccTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTg                                <  2:383481/148‑1 (MQ=255)

                                                                                                                      gAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGttt                             >  2:2722923/1‑148 (MQ=255)

                                                                                                                       aGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTa                            >  2:2745021/1‑148 (MQ=255)

                                                                                                                              tccaCAGCTTCTCGCGGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGc                     <  2:116059/145‑1 (MQ=255)

                                                                                                                              tCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGc                     >  2:2905509/1‑148 (MQ=255)

                                                                                                                                aaCAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCa                   <  1:2614054/148‑1 (MQ=255)

                                                                                                                                   aGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATgg                >  2:1140538/1‑148 (MQ=255)

                                                                                                                                   aGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATgg                >  2:1140552/1‑148 (MQ=255)

                                                                                                                                    gCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGc               >  1:2627593/1‑148 (MQ=255)

                                                                                                                                    gCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGc               >  1:3294663/1‑148 (MQ=255)

                                                                                                                                     cTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCg              >  1:1828175/1‑148 (MQ=255)

                                                                                                                                       tctcGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaa            >  1:922627/1‑148 (MQ=255)

                                                                                                                                       gcgcGCTGAGCGGGAAAATCGTCGATAACCTCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCACCGGTTGGCCTGTTTACAGGTGCCATGGCGaa            <  2:3019866/145‑1 (MQ=255)

                                                                                                                                        ctcGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaaa           >  2:1910029/1‑148 (MQ=255)

                                                                                                                                          cGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaaga         <  2:161977/148‑1 (MQ=255)

                                                                                                                                            cTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaagaag       >  1:161079/1‑148 (MQ=255)

                                                                                                                                             tGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGt      <  2:3247216/148‑1 (MQ=255)

TGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTA  >  CP009273/3535225‑3535517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGT  >  CP009273/3535222‑3535519

TCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAG                                                                                                                                                      <  SRR19973065.518408/150‑1 (MQ=60)

  ATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGG                                                                                                                                                    >  SRR19973065.386162/1‑150 (MQ=60)

  ATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGG                                                                                                                                                    >  SRR19973065.1618665/1‑150 (MQ=60)

   TGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGA                                                                                                                                                   >  SRR19973065.1030520/1‑150 (MQ=60)

   TGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGA                                                                                                                                                   >  SRR19973065.1030581/1‑150 (MQ=60)

   TGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGA                                                                                                                                                   >  SRR19973065.3096732/1‑150 (MQ=60)

   TGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGA                                                                                                                                                   <  SRR19973065.2760150/150‑1 (MQ=60)

   TGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGA                                                                                                                                                   <  SRR19973065.2340794/150‑1 (MQ=60)

   TGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCGGAGAGGGA                                                                                                                                                   >  SRR19973065.3097441/1‑150 (MQ=60)

    GGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAA                                                                                                                                                  <  SRR19973065.2286336/150‑1 (MQ=60)

    GGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAA                                                                                                                                                  <  SRR19973065.1643449/150‑1 (MQ=60)

     GAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAA                                                                                                                                                 <  SRR19973065.2744375/150‑1 (MQ=60)

      AGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAA                                                                                                                                                >  SRR19973065.2348479/1‑150 (MQ=60)

       GTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAT                                                                                                                                               <  SRR19973065.1604724/150‑1 (MQ=60)

       GTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAT                                                                                                                                               <  SRR19973065.2200501/150‑1 (MQ=60)

           CCGGTCTATCTTGTACCACACGTTAAAAGCCTGATTATCAAGACCTGGCAGAGTCTGAAAGGCTTCGTTCTGCGTGATGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAATATCGTC                                                                                                                                           <  SRR19973065.2810321/150‑1 (MQ=60)

           CCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTC                                                                                                                                           <  SRR19973065.2810121/150‑1 (MQ=60)

           CCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTC                                                                                                                                           <  SRR19973065.3412536/150‑1 (MQ=60)

            CGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCG                                                                                                                                          <  SRR19973065.2851250/150‑1 (MQ=60)

               TCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATA                                                                                                                                       <  SRR19973065.1720741/150‑1 (MQ=60)

               TCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATA                                                                                                                                       <  SRR19973065.3381191/150‑1 (MQ=60)

                  ATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACA                                                                                                                                    <  SRR19973065.2718184/150‑1 (MQ=60)

                  ATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACA                                                                                                                                    <  SRR19973065.2628676/150‑1 (MQ=60)

                    CATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATC                                                                                                                                  >  SRR19973065.100501/1‑150 (MQ=60)

                     ATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCA                                                                                                                                 >  SRR19973065.1486679/1‑150 (MQ=60)

                     ATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCA                                                                                                                                 >  SRR19973065.1280834/1‑150 (MQ=60)

                     ATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCA                                                                                                                                 >  SRR19973065.1486860/1‑150 (MQ=60)

                     ATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCA                                                                                                                                 >  SRR19973065.1486680/1‑150 (MQ=60)

                     ATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCA                                                                                                                                 >  SRR19973065.3038367/1‑150 (MQ=60)

                       GTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAAC                                                                                                                               <  SRR19973065.2659761/150‑1 (MQ=60)

                                GTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCG                                                                                                                      >  SRR19973065.2511757/1‑150 (MQ=60)

                                 TTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGC                                                                                                                     >  SRR19973065.258614/1‑150 (MQ=60)

                                   AAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTG                                                                                                                   >  SRR19973065.2996666/1‑150 (MQ=60)

                                   AAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTG                                                                                                                   >  SRR19973065.3465740/1‑150 (MQ=60)

                                         CTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCC                                                                                                             >  SRR19973065.1951867/1‑150 (MQ=60)

                                         CTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCC                                                                                                             >  SRR19973065.381839/1‑150 (MQ=60)

                                         CTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCC                                                                                                             >  SRR19973065.1953001/1‑150 (MQ=60)

                                         CTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTATCCTGAGCGCTTTCAACAGCTTCTCGATGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCC                                                                                                             >  SRR19973065.381780/1‑150 (MQ=60)

                                            ATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTC                                                                                                          >  SRR19973065.163306/1‑150 (MQ=60)

                                                  CAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGG                                                                                                    >  SRR19973065.164100/1‑150 (MQ=60)

                                                  CAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGG                                                                                                    >  SRR19973065.163936/1‑150 (MQ=60)

                                                   AGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGG                                                                                                   >  SRR19973065.1156065/1‑150 (MQ=60)

                                                   AGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGG                                                                                                   >  SRR19973065.2225294/1‑150 (MQ=60)

                                                    GACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGT                                                                                                  >  SRR19973065.650064/1‑150 (MQ=60)

                                                      CCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGA                                                                                                >  SRR19973065.1671240/1‑150 (MQ=60)

                                                        gggccGCGTCTGAAGGGCTTCGTTCTGCGTGGTGGTAATGTGAACATCATCGGCAGAATTTTCCTGAGCGCTTTCAACCGCTTCTCGCTGCGCGGGAAAATCGTCTATAACAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATC                                                                                              <  SRR19973065.1764945/145‑1 (MQ=60)

                                                        TGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATC                                                                                              >  SRR19973065.3436359/1‑150 (MQ=60)

                                                           CAGGGTCTTAATGGCTTGGTTCTGCGTGGTGTTAATGTGATCATCATCGCCCGCTTTTTCCTGAGCGCTTTCAACCGCTTCTCGCTGAGAGGTAAATTCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACC                                                                                           <  SRR19973065.2824577/150‑1 (MQ=60)

                                                            AGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCC                                                                                          >  SRR19973065.1221840/1‑150 (MQ=60)

                                                            AGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCATCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCC                                                                                          >  SRR19973065.945912/1‑150 (MQ=60)

                                                             GCGTCTGAACGGTTTGGTTCTGCGTGCTGGTAAAGTGATCATCCTCGTCAGAATTTTCCGGCGCGCTTTAAACAGCTTCTCGCTGTGAGGTAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCC                                                                                         <  SRR19973065.2057206/150‑1 (MQ=60)

                                                             GCGGCTGAAAGGCTTGGTACTGCGTGCTGGTAAAGTGATCAACATCGTCAGCATTTGCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGTAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCC                                                                                         <  SRR19973065.3417819/150‑1 (MQ=60)

                                                               GTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGG                                                                                       >  SRR19973065.174470/1‑150 (MQ=60)

                                                               GTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGG                                                                                       >  SRR19973065.1962774/1‑150 (MQ=60)

                                                                   GAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTT                                                                                   >  SRR19973065.659256/1‑150 (MQ=60)

                                                                       GGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAG                                                                               >  SRR19973065.740030/1‑150 (MQ=60)

                                                                       GGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAG                                                                               >  SRR19973065.2860401/1‑150 (MQ=60)

                                                                         tttgtttctgggtggttttaatgttataATCATCGTCATCTTTTTCCTGCGCGATTACAACAGCTTCTCGCTGAGTGGTAAAATCGTCTATAACAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCC                                                                             <  SRR19973065.3432513/122‑1 (MQ=60)

                                                                         CTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCTCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCC                                                                             >  SRR19973065.2759089/1‑150 (MQ=60)

                                                                            CTTTCTGCGTGCTGTTAATGTTATCATCATGGTCAGATTTTTCCTGAGCGCTTACACCAGCTTCTCGCGGAGTGGTAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAAT                                                                          <  SRR19973065.863894/150‑1 (MQ=60)

                                                                            CGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAAT                                                                          >  SRR19973065.2519266/1‑150 (MQ=60)

                                                                                    GTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGC                                                                  >  SRR19973065.1696092/1‑150 (MQ=60)

                                                                                    GTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGC                                                                  >  SRR19973065.1696410/1‑150 (MQ=60)

                                                                                       CTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATG                                                               >  SRR19973065.1880802/1‑150 (MQ=60)

                                                                                       CTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATG                                                               >  SRR19973065.1881058/1‑150 (MQ=60)

                                                                                           TAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGA                                                           >  SRR19973065.2168770/1‑150 (MQ=60)

                                                                                           TAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCAGGAAGA                                                           >  SRR19973065.440819/1‑150 (MQ=60)

                                                                                            AAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGAT                                                          >  SRR19973065.2495876/1‑150 (MQ=60)

                                                                                                 GATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTG                                                     >  SRR19973065.2630684/1‑150 (MQ=60)

                                                                                                    CATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCA                                                  >  SRR19973065.3363500/1‑150 (MQ=60)

                                                                                                     aacatcttaagatTTTTCCTGCGCGCTTTCAACTGCTTCTCGCTGAGTGGTAAATTCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAG                                                 <  SRR19973065.1835235/137‑1 (MQ=60)

                                                                                                     ATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAG                                                 >  SRR19973065.1420836/1‑150 (MQ=60)

                                                                                                     ATCAGCGTCAGATTTTTCCTGAGCGCTTTCAACTGCTTCTCGCTGAGTGTAAAAATCGTCGAAAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAG                                                 <  SRR19973065.2452363/150‑1 (MQ=60)

                                                                                                      tcttgttcagatttttcgtgtgggttttcaactggTTCTCGCTGTGTGGGAAAATGGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGG                                                <  SRR19973065.2060835/115‑1 (MQ=60)

                                                                                                        agcggcagatTTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGTAAAATCGTCTAAAACACCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCA                                              <  SRR19973065.3008181/140‑1 (MQ=60)

                                                                                                             CAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGT                                         >  SRR19973065.1259028/1‑150 (MQ=60)

                                                                                                              tgatTTTTCCTGAGCGCTTAAAACTGCTTCTCGCTTTGTGGTAAAATCGTCTATAACAACCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTT                                        <  SRR19973065.809053/146‑1 (MQ=60)

                                                                                                               GCTTTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGTGGGAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTG                                       <  SRR19973065.1388697/150‑1 (MQ=60)

                                                                                                               GCTTTGTCCTGTGCGCTTTCAACAGCTTCTCGCTGTGAGGGAAAATGGTCTATAACACCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTG                                       <  SRR19973065.3143193/150‑1 (MQ=60)

                                                                                                                CATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGG                                      >  SRR19973065.2639376/1‑150 (MQ=60)

                                                                                                                       CTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTT                               >  SRR19973065.1374179/1‑150 (MQ=60)

                                                                                                                         GAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTAC                             >  SRR19973065.246224/1‑150 (MQ=60)

                                                                                                                                   AACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATG                   <  SRR19973065.2630255/150‑1 (MQ=60)

                                                                                                                                   AACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATG                   >  SRR19973065.189904/1‑150 (MQ=60)

                                                                                                                                     CAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGC                 >  SRR19973065.3314727/1‑150 (MQ=60)

                                                                                                                                     CAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGC                 >  SRR19973065.2643846/1‑150 (MQ=60)

                                                                                                                                      AGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCG                >  SRR19973065.1840217/1‑150 (MQ=60)

                                                                                                                                        CTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAA              >  SRR19973065.927804/1‑150 (MQ=60)

                                                                                                                                        CTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAA              >  SRR19973065.928672/1‑150 (MQ=60)

                                                                                                                                        CTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCAGGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAA              >  SRR19973065.3517052/1‑150 (MQ=60)

                                                                                                                                             CGCTGAGAGGTAAAATCGTCGATAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAG         <  SRR19973065.2647368/150‑1 (MQ=60)

                                                                                                                                             CGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAG         >  SRR19973065.162404/1‑150 (MQ=60)

                                                                                                                                             CGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAG         >  SRR19973065.2572329/1‑150 (MQ=60)

                                                                                                                                                TGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGG      >  SRR19973065.2327745/1‑150 (MQ=60)

                                                                                                                                                 GAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTACATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGT     >  SRR19973065.2267362/1‑150 (MQ=60)

                                                                                                                                                   GAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAG   >  SRR19973065.3010977/1‑150 (MQ=60)

                                                                                                                                                    CGGTAAAATCGTCTATAACACCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGT  <  SRR19973065.2766995/150‑1 (MQ=60)

TCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGT  >  CP009273/3535222‑3535519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`