Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,535,370 C→A 84.8% S535R (AGC→AGA)  feoB → fused ferrous iron transporter, protein B: GTP‑binding protein/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,535,3700CA84.8% 434.9 / 88.8 227S535R (AGC→AGA) feoBfused ferrous iron transporter, protein B: GTP‑binding protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base C (23/11);  new base A (117/49);  total (140/87)
Fisher's exact test for biased strand distribution p-value = 8.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

ATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGG  >  CP009273/3535224‑3535515
                                                                                                                                                   |                                                                                                                                                 
aTGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAgg                                                                                                                                                 >  2:181445/1‑149 (MQ=255)
   gAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGaa                                                                                                                                              <  2:3882098/149‑1 (MQ=255)
   gAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGaa                                                                                                                                              >  2:2482905/1‑149 (MQ=255)
    aGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGaaa                                                                                                                                             >  1:4919/1‑149 (MQ=255)
     gTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTTAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGaaaa                                                                                                                                            <  1:4597689/149‑1 (MQ=255)
     gTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGaaaa                                                                                                                                            >  1:3524676/1‑149 (MQ=255)
      ttGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAt                                                                                                                                           <  2:264338/149‑1 (MQ=255)
      ttGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAt                                                                                                                                           <  2:3924318/149‑1 (MQ=255)
           ggTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtcgtcg                                                                                                                                      >  2:1265561/1‑149 (MQ=255)
            gTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGa                                                                                                                                     <  1:2042942/149‑1 (MQ=255)
             tCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGAt                                                                                                                                    >  2:2207116/1‑149 (MQ=255)
              cTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATa                                                                                                                                   >  2:852657/1‑149 (MQ=255)
               tATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATaa                                                                                                                                  <  2:4468183/149‑1 (MQ=255)
                aTCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGACAATCGTCGATAAc                                                                                                                                 >  1:2648984/1‑149 (MQ=255)
                 tCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACa                                                                                                                                <  2:4050223/149‑1 (MQ=255)
                   aTGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATc                                                                                                                              <  1:4413720/149‑1 (MQ=255)
                    tGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCa                                                                                                                             >  1:4242639/1‑149 (MQ=255)
                    tGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCa                                                                                                                             >  1:4242619/1‑149 (MQ=255)
                    tGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCa                                                                                                                             >  1:1216508/1‑149 (MQ=255)
                      tACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAAc                                                                                                                           >  2:1451677/1‑149 (MQ=255)
                        ccACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGa                                                                                                                         >  2:1953700/1‑149 (MQ=255)
                         cacaCGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGAc                                                                                                                        <  1:1523895/149‑1 (MQ=255)
                            acGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCg                                                                                                                     >  2:2802788/1‑149 (MQ=255)
                            acGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCg                                                                                                                     >  1:3960069/1‑149 (MQ=255)
                               ttAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGgcg                                                                                                                  >  2:2958122/1‑149 (MQ=255)
                                tAAAAGGCTTATTATCCATACCTGGAAGCGTCTGAATGGCTTCGTTCTGCTTGATTTTAAAGTTATCATCTTGGTATGAA‑TTTTCGTTTGCTCTTTCAACTGCTTATCGATGAGCGGGAAATTCTTCTATAACATCAACGACTCGgcgc                                                                                                                 <  2:67089/149‑1 (MQ=255)
                                   aaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTgg                                                                                                              >  1:4024705/1‑149 (MQ=255)
                                   aaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTgg                                                                                                              >  1:1637208/1‑149 (MQ=255)
                                   aaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTgg                                                                                                              >  1:993670/1‑149 (MQ=255)
                                   aaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTgg                                                                                                              >  2:936581/1‑149 (MQ=255)
                                     gCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcg                                                                                                            >  2:456486/1‑149 (MQ=255)
                                      ccTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgt                                                                                                           >  1:4179011/1‑149 (MQ=255)
                                         gATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccg                                                                                                        >  2:3068328/1‑149 (MQ=255)
                                          aTTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccgt                                                                                                       >  2:732630/1‑149 (MQ=255)
                                          aTTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccgt                                                                                                       >  1:1910669/1‑149 (MQ=255)
                                           ttATCCAGACCTGGCAGCGTCTGAAAGGCTGCGTTCTGCGTGCTGGTAAAGTTATCATAATCGTCAGCT‑TTTTCCTGAGCGCTTACAACAGCTTCTCGCTGAGAGGTAAAATGGTCGATAACATCAACGACTCGGCGCTGGcgtccgtc                                                                                                      <  2:3407252/149‑1 (MQ=255)
                                            tATCCAGACCTGGCAGCGTCTTAAAGGCTTCTTTCTGCGTGCTGGTAAAGTGATAATAATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGTAAAATCGTCGATGACATCCACGACTCGGCGCTGGCGTCCGTCa                                                                                                     <  1:1335006/149‑1 (MQ=255)
                                            tATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCa                                                                                                     >  1:339710/1‑149 (MQ=255)
                                              tCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGc                                                                                                   >  2:1115525/1‑149 (MQ=255)
                                              tCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGc                                                                                                   >  2:61160/1‑149 (MQ=255)
                                               ccAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGcc                                                                                                  >  2:1883333/1‑149 (MQ=255)
                                                     cTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGACAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGa                                                                                            >  2:4024019/1‑149 (MQ=255)
                                                     cTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGa                                                                                            >  1:1461771/1‑149 (MQ=255)
                                                      tGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGAt                                                                                           >  1:464947/1‑149 (MQ=255)
                                                         cAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGTTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACCGCTTCTCGCTGAGAGGGAAATTCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCAc                                                                                        <  2:4542931/149‑1 (MQ=255)
                                                           gCGTCTGAATGGCTTCGTTCTGCGTGCTGGTAATGTGATAATCATCGTCAGCA‑TTTTCCTGAGCGATTTCAACAGCTTCTCGCTGAGTGGGAAAATCGTCTATAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCAccc                                                                                      <  2:1635001/149‑1 (MQ=255)
                                                           gCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAACGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACCGCTTCTCGCTGAGTGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCAccc                                                                                      <  2:4744919/149‑1 (MQ=255)
                                                           gCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCAccc                                                                                      <  2:4745385/149‑1 (MQ=255)
                                                              tCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCgg                                                                                   >  1:2864130/1‑149 (MQ=255)
                                                              tCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCgg                                                                                   >  1:3033356/1‑149 (MQ=255)
                                                                tGAAAGGCTTCGTTCTGCGTGCTGGTAATGTGATAATCATCGACCGCA‑TTTTCCTGAGCGCTTACAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTc                                                                                 <  2:3280679/149‑1 (MQ=255)
                                                                  aaaGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCtt                                                                               >  1:1811738/1‑149 (MQ=255)
                                                                  aaaGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCtt                                                                               >  2:891636/1‑149 (MQ=255)
                                                                    cggCTTCGTTCTGCGTGGTGGTAATGTTATAATAATCGTCAGCT‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGGTGTGTGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCa                                                                             <  1:1076897/148‑1 (MQ=255)
                                                                     ggCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCaa                                                                            >  1:3890432/1‑149 (MQ=255)
                                                                        ttgtttCTGGGTGCTTGTAATTTTATCAACATCGTCTGAT‑TTTTCCTGAGCGATTACAACAGCTTCTCGGTGTGTGGTAAATTGGTCTATAACAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGcc                                                                         <  1:2926101/145‑1 (MQ=255)
                                                                           gTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAAt                                                                      >  2:3562476/1‑149 (MQ=255)
                                                                             tCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTTCCAGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACAGCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAAtt                                                                     <  2:1051369/149‑1 (MQ=255)
                                                                                gCGTGCTGTTAATGTGATCATCTTCGTCAGCA‑TTTTCCTGAGCGCTTTCAACCGCTTCTCGGTGAGAGGTAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCg                                                                 <  2:2406509/149‑1 (MQ=255)
                                                                                   tGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGc                                                              >  1:886724/1‑149 (MQ=255)
                                                                                   tGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGc                                                              >  1:3715949/1‑149 (MQ=255)
                                                                                   tGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGc                                                              >  1:3219504/1‑149 (MQ=255)
                                                                                   tGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGc                                                              >  1:3276516/1‑149 (MQ=255)
                                                                                      tGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATg                                                           >  2:3159934/1‑149 (MQ=255)
                                                                                            aGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATa                                                     >  2:2772261/1‑149 (MQ=255)
                                                                                                   atcatcGTCAGAT‑TTTTCCTGAGCGCTTTCAACCGGTTCTCGCTGAGTGGTAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggca                                              <  1:764021/149‑1 (MQ=255)
                                                                                                     catcGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggcagg                                            >  1:3558046/1‑149 (MQ=255)
                                                                                                     aatcGTCATCA‑TTTTCCTGAGCGATTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggcagg                                            <  1:324980/148‑1 (MQ=255)
                                                                                                         gTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAAc                                        >  1:2375298/1‑149 (MQ=255)
                                                                                                          tCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCCTGAAGATAACTGGCAGGCAACg                                       >  1:414331/1‑149 (MQ=255)
                                                                                                          tCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACg                                       >  1:3626857/1‑149 (MQ=255)
                                                                                                            aGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCCTGAAGATCACTGGCAGGCAACGGt                                     >  2:4173963/1‑149 (MQ=255)
                                                                                                             gCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGtt                                    >  1:4447257/1‑149 (MQ=255)
                                                                                                             gCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGtt                                    >  1:4447487/1‑149 (MQ=255)
                                                                                                             gCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGtt                                    >  1:2986858/1‑149 (MQ=255)
                                                                                                             gCA‑TTTGCCGGAGCGCTTTCAACAGCTTCACGCTGCGAGTGAAAATCGTCGATAACAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGtt                                    <  2:2896173/149‑1 (MQ=255)
                                                                                                             agatttttCGTGCGCGATTTAAACCGTTTCTGGGTGAGGGGTAAATTGGTCTATAACTTCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGt                                     <  1:3593613/145‑1 (MQ=255)
                                                                                                              cA‑TTTTCCTGGGCGGTTTCAACTGCTTCTCGCGGGGTGGGAAAATCGTCGATAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTg                                   <  2:2140150/149‑1 (MQ=255)
                                                                                                                 tttgcgtGAGCTTTTTAAACCGTTTCTCGCTGTGTGTGAAAATCGTCTATAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGc                                 <  1:4478017/143‑1 (MQ=255)
                                                                                                                    tCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTg                              >  1:2049327/1‑149 (MQ=255)
                                                                                                                        gAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTa                          >  1:1737610/1‑149 (MQ=255)
                                                                                                                        gAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTa                          >  1:3969201/1‑149 (MQ=255)
                                                                                                                          gcgcTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACa                        >  1:387550/1‑149 (MQ=255)
                                                                                                                             cTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGt                     >  2:4775217/1‑149 (MQ=255)
                                                                                                                              tttCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTg                    >  1:4049789/1‑149 (MQ=255)
                                                                                                                              tttCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTg                    >  2:4300721/1‑149 (MQ=255)
                                                                                                                              tttCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTg                    >  1:4730151/1‑149 (MQ=255)
                                                                                                                                tCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGcc                  >  2:3217240/1‑149 (MQ=255)
                                                                                                                                   aCAGCTTCTCGCTGTGTGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATg               <  1:2301100/149‑1 (MQ=255)
                                                                                                                                    cAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATgg              >  1:1035221/1‑149 (MQ=255)
                                                                                                                                     aGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGc             >  1:1504911/1‑149 (MQ=255)
                                                                                                                                     aGCTCCGCGCTGAGTGGTAAAAGCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGc             <  1:1770625/149‑1 (MQ=255)
                                                                                                                                      gCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCg            >  2:3173359/1‑149 (MQ=255)
                                                                                                                                           tcGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaaga       >  1:3838133/1‑149 (MQ=255)
                                                                                                                                            cGCTGAGAGGGAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaagaa      <  2:1807278/149‑1 (MQ=255)
                                                                                                                                            cGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaagaa      >  1:2466876/1‑149 (MQ=255)
                                                                                                                                              cTGAGAGGGAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGt    <  2:525208/149‑1 (MQ=255)
                                                                                                                                              cTGAGAGGGAAAAGCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGt    <  2:3456493/149‑1 (MQ=255)
                                                                                                                                               tGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGACGTg   >  2:3335976/1‑149 (MQ=255)
                                                                                                                                                gagaGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTgg  >  2:438225/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCA‑TTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGG  >  CP009273/3535224‑3535515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGT  >  CP009273/3535221‑3535519
                                                                                                                                                     |                                                                                                                                                     
GTCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGA                                                                                                                                                       >  SRR19973071.3264572/1‑150 (MQ=60)
GTCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGA                                                                                                                                                       >  SRR19973071.2547438/1‑150 (MQ=60)
GTCATGGAGTTGACGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCAAGACCTGGCAGCGTCTGAAAGGCTGCGTTCAGCGTGCTGGTAAAGAGATCATCATCGTCAGCATTTTCCTGAGCGCTGTCAACAGCTTCTCGCTGAGA                                                                                                                                                       >  SRR19973071.2921672/1‑150 (MQ=60)
  CATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGG                                                                                                                                                     <  SRR19973071.2387095/150‑1 (MQ=60)
  CATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGG                                                                                                                                                     <  SRR19973071.2387026/150‑1 (MQ=60)
      GAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAA                                                                                                                                                 >  SRR19973071.4963/1‑150 (MQ=60)
       AGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAA                                                                                                                                                >  SRR19973071.3537021/1‑150 (MQ=60)
        GTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTTAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAT                                                                                                                                               <  SRR19973071.4614080/150‑1 (MQ=60)
         TTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATC                                                                                                                                              <  SRR19973071.3802696/150‑1 (MQ=60)
          TGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCG                                                                                                                                             >  SRR19973071.3026614/1‑150 (MQ=60)
            CCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTC                                                                                                                                           >  SRR19973071.1599928/1‑150 (MQ=60)
            CCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTC                                                                                                                                           >  SRR19973071.1790168/1‑150 (MQ=60)
               GTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGAT                                                                                                                                        <  SRR19973071.3586888/150‑1 (MQ=60)
               GTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGAT                                                                                                                                        <  SRR19973071.2050430/150‑1 (MQ=60)
               GTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGAT                                                                                                                                        >  SRR19973071.1814012/1‑150 (MQ=60)
                TCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATA                                                                                                                                       <  SRR19973071.215130/150‑1 (MQ=60)
                 CTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAA                                                                                                                                      >  SRR19973071.2527363/1‑150 (MQ=60)
                  TATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGACAATCGTCGATAAC                                                                                                                                     >  SRR19973071.2658502/1‑150 (MQ=60)
                   ATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACA                                                                                                                                    >  SRR19973071.2981516/1‑150 (MQ=60)
                     CATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATC                                                                                                                                  <  SRR19973071.386682/150‑1 (MQ=60)
                     CATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATC                                                                                                                                  >  SRR19973071.911598/1‑150 (MQ=60)
                      ATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCTGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATAATCTTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTTAGAGGGAAAATCGTCGATAACATCA                                                                                                                                 <  SRR19973071.4429361/150‑1 (MQ=60)
                      ATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCA                                                                                                                                 >  SRR19973071.2739982/1‑150 (MQ=60)
                      ATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCA                                                                                                                                 >  SRR19973071.4257691/1‑150 (MQ=60)
                      ATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCA                                                                                                                                 >  SRR19973071.4257701/1‑150 (MQ=60)
                      ATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCA                                                                                                                                 >  SRR19973071.4257712/1‑150 (MQ=60)
                      ATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCA                                                                                                                                 <  SRR19973071.4429261/150‑1 (MQ=60)
                      ATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCA                                                                                                                                 >  SRR19973071.1220723/1‑150 (MQ=60)
                          ACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGA                                                                                                                             >  SRR19973071.2053913/1‑150 (MQ=60)
                          ACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGA                                                                                                                             <  SRR19973071.1887702/150‑1 (MQ=60)
                           CCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAATTCGTCGATAACATCAACGAC                                                                                                                            <  SRR19973071.1706104/150‑1 (MQ=60)
                            CACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAATGTGATCATCATCGTCAGAATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACT                                                                                                                           <  SRR19973071.129154/150‑1 (MQ=60)
                            CACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACT                                                                                                                           <  SRR19973071.1529385/150‑1 (MQ=60)
                              CACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCG                                                                                                                         >  SRR19973071.3973968/1‑150 (MQ=60)
                                   TAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCT                                                                                                                    >  SRR19973071.148786/1‑150 (MQ=60)
                                     AAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGG                                                                                                                  >  SRR19973071.1643140/1‑150 (MQ=60)
                                     AAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGG                                                                                                                  >  SRR19973071.4038870/1‑150 (MQ=60)
                                     AAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGG                                                                                                                  >  SRR19973071.4038880/1‑150 (MQ=60)
                                     AAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGG                                                                                                                  >  SRR19973071.4038884/1‑150 (MQ=60)
                                     AAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGG                                                                                                                  >  SRR19973071.997283/1‑150 (MQ=60)
                                      AAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGC                                                                                                                 >  SRR19973071.1075866/1‑150 (MQ=60)
                                        GCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGT                                                                                                               >  SRR19973071.4193889/1‑150 (MQ=60)
                                        GCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGT                                                                                                               >  SRR19973071.4193955/1‑150 (MQ=60)
                                          CTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCC                                                                                                             >  SRR19973071.1962940/1‑150 (MQ=60)
                                            GATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGT                                                                                                           >  SRR19973071.1917673/1‑150 (MQ=60)
                                             ATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTC                                                                                                          >  SRR19973071.2911298/1‑150 (MQ=60)
                                              TTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCA                                                                                                         >  SRR19973071.340841/1‑150 (MQ=60)
                                               taccccgcCCTGGCCGCGTCTTAACGGCTTCGTTCTGCGGGCTGTTAATGTGAACATCAACGTCAGCTTTTTCCTGCGCGATTTCACCCGCTTCTCGCTGAGTGGCAAATTCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAG                                                                                                        <  SRR19973071.1340002/142‑1 (MQ=50)
                                               taacccgacctggcagggtcttaacgggttctttctggggGCTGGTAATGTGATCATCTTCGTCATATTTTTCCTGCGCGCTTCAAACCGCTTCTCGCTGAGTGGCAAAATGGTCTATAAAAACAACGACTCGGCGCTGGCGTCCGTCAG                                                                                                        <  SRR19973071.1339767/110‑1 (MQ=22)
                                               TATCCAGACCTGGCAGCGTCTTAAAGGCTTCTTTCTGCGTGCTGGTAAAGTGATAATAATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGTAAAATCGTCGATGACATCCACGACTCGGCGCTGGCGTCCGTCAG                                                                                                        <  SRR19973071.1339912/150‑1 (MQ=60)
                                                       cggggcagggttttattggatttgttgtgggtgctgttaatgttataagaagcttctgatTTTTCCTGCGGGCTTCCAACAGGTTCTCGCTGATTGGTAAAATCTTCGATAAAAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGA                                                                                                <  SRR19973071.2488011/90‑1 (MQ=35)
                                                       CCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGA                                                                                                >  SRR19973071.1467107/1‑150 (MQ=60)
                                                        CTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGAT                                                                                               >  SRR19973071.466628/1‑150 (MQ=60)
                                                        CTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGAT                                                                                               >  SRR19973071.466641/1‑150 (MQ=60)
                                                         TGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATC                                                                                              >  SRR19973071.3540833/1‑150 (MQ=60)
                                                         TGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATC                                                                                              >  SRR19973071.4635623/1‑150 (MQ=60)
                                                         TGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATC                                                                                              >  SRR19973071.3540832/1‑150 (MQ=60)
                                                          GGCTGCGTCTGAATGGCTTGGTTCTGCGTGCTGGTAATGTTATCATCATCGACAGAATTTTCCTGAGCGATTTCAACCGCTACTCGCTGAGAGGTAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCA                                                                                             <  SRR19973071.1570239/150‑1 (MQ=60)
                                                          GGCAGCGTCTGAACGGCTTCTTTCTGCGTGCTGGTAATGTTATCATCATCTTCAGATTTTTCCTGAGCGATTTAAACAGCTTATCGCTTAGTGGTAAAATCGTCTATAACAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCA                                                                                             <  SRR19973071.1570196/150‑1 (MQ=60)
                                                          GGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCA                                                                                             >  SRR19973071.2221997/1‑150 (MQ=60)
                                                               cttcttaaggggttggttgggggtgctttaaaagttaacatcatcttcttatttttcgtgcgcgcttaaacCAGCTTCTCGCTGTGTGGGAAATTCGTCTAAAACAACCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCT                                                                                        <  SRR19973071.3644382/79‑1 (MQ=46)
                                                               cgttttaatGGCTTCGTTCGGGGTGCTGTTAATGTTATCATCATCGTCAGCTTTTTCCTGCGCGCTTAAAACTGCTTCTCGCTGAGTGGTAAATTCGTCTATAACAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCT                                                                                        <  SRR19973071.3644270/141‑1 (MQ=60)
                                                                GTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGG                                                                                       >  SRR19973071.3044149/1‑150 (MQ=60)
                                                                GTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGG                                                                                       >  SRR19973071.3942660/1‑150 (MQ=60)
                                                                GTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGG                                                                                       >  SRR19973071.2874332/1‑150 (MQ=60)
                                                                    GAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTT                                                                                   >  SRR19973071.1818484/1‑150 (MQ=60)
                                                                    GAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTT                                                                                   >  SRR19973071.2483503/1‑150 (MQ=60)
                                                                       GGGCTTCGTTCTGCGTGCTGTTAAAGTGATCAACATCTTCCCATTTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGCGTGGTAAATTGGTCGTTAACAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAA                                                                                <  SRR19973071.1080818/150‑1 (MQ=60)
                                                                       CGGCTTCGTTCTGCGTGGTGGTAATGTTATAATAATCGTCAGCTTTTTCCTGAGCGCTTTCAACAGCTTCTCGGTGTGTGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAA                                                                                <  SRR19973071.1080724/150‑1 (MQ=60)
                                                                       AGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAA                                                                                >  SRR19973071.4428635/1‑150 (MQ=60)
                                                                       AGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAA                                                                                >  SRR19973071.3904168/1‑150 (MQ=60)
                                                                        GGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAG                                                                               >  SRR19973071.3328006/1‑150 (MQ=60)
                                                                           ttgtttctgggtgcttgtaattttatcaacatcgtctgatTTTTCCTGAGCGATTACAACAGCTTCTCGGTGTGTGGTAAATTGGTCTATAACAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCA                                                                            <  SRR19973071.2936586/110‑1 (MQ=60)
                                                                              tttctgcttgttgttaatgttataataatcttctgatttttcctgcgctcttacacCAGCTTCTCGCTGATTGGCAAATTCGTCTATAACAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATT                                                                         <  SRR19973071.3673160/94‑1 (MQ=60)
                                                                                    cgtggtgttaatgttATCATCATCGTCTGCTTTTTCCTGAGCGCTTTCAACAGATTCTCGCTGTGAGGTAAAATCGTCGTTAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTG                                                                   <  SRR19973071.4004746/135‑1 (MQ=60)
                                                                                     GTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGC                                                                  >  SRR19973071.3288115/1‑150 (MQ=60)
                                                                                     GTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGC                                                                  >  SRR19973071.889908/1‑150 (MQ=60)
                                                                                     GTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGC                                                                  >  SRR19973071.3230809/1‑150 (MQ=60)
                                                                                     GTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGC                                                                  >  SRR19973071.3729109/1‑150 (MQ=60)
                                                                                        CTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATG                                                               >  SRR19973071.4725037/1‑150 (MQ=60)
                                                                                        CTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATG                                                               >  SRR19973071.557003/1‑150 (MQ=60)
                                                                                         tgtaatttttataataATCGTCAGAATTTTCCTGTGCGATTTCAACAGCTTATCGCTTTTTGGTAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGA                                                              <  SRR19973071.1940437/134‑1 (MQ=60)
                                                                                         TGTTAAAGTTATCATCATCGTCATCATTTTCCTGAGCGCTTTCAACCGATTCTCGCTGAGTGGTAAAATCTTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGA                                                              <  SRR19973071.1512940/150‑1 (MQ=60)
                                                                                          GGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAA                                                             >  SRR19973071.3273211/1‑150 (MQ=60)
                                                                                                      ATCATCGTCAGATTTTTCCTGAGCGCTTTCAACCGGTTCTCGCTGAGTGGTAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAG                                                 <  SRR19973071.766614/150‑1 (MQ=60)
                                                                                                       aaATCGTCAGCATTTTCCTGAGCGCTTACAACAGCTTCTCGTTGAGTGGTAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGG                                                <  SRR19973071.2981193/148‑1 (MQ=60)
                                                                                                       TCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGG                                                >  SRR19973071.3570447/1‑150 (MQ=60)
                                                                                                        CATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGCCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGC                                               >  SRR19973071.3277716/1‑150 (MQ=60)
                                                                                                        AATCGTCATCATTTTCCTGAGCGATTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGC                                               <  SRR19973071.326087/150‑1 (MQ=60)
                                                                                                         tgcgtcttctTTTTCCTGTGCTCTTTAAACTGCTTCCCGCTGAGTGGTAAAATCGTCTATAAAATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCA                                              <  SRR19973071.4455598/140‑1 (MQ=60)
                                                                                                         atcttcagatTTTTCCTGAGCGCTTAAAACAGCTTCTCGCTGAGTGGGAAAATCGTCGATAACAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCA                                              <  SRR19973071.2329305/140‑1 (MQ=60)
                                                                                                         ATCTTAAGCATTTTCCTGAGCGCTTTCAACAGATTCTCGCTGAGTGTTAAATTCGTATATAACAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCA                                              <  SRR19973071.4210505/150‑1 (MQ=60)
                                                                                                           CGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAAC                                            >  SRR19973071.2383872/1‑150 (MQ=60)
                                                                                                            GTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCCTGAAGATAACTGGCAGGCAACG                                           >  SRR19973071.415698/1‑150 (MQ=60)
                                                                                                            GTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACG                                           >  SRR19973071.3639591/1‑150 (MQ=60)
                                                                                                            GTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACG                                           >  SRR19973071.3639575/1‑150 (MQ=60)
                                                                                                            GTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACG                                           >  SRR19973071.3639582/1‑150 (MQ=60)
                                                                                                               AGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTT                                        >  SRR19973071.4462981/1‑150 (MQ=60)
                                                                                                               AGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTT                                        >  SRR19973071.4463039/1‑150 (MQ=60)
                                                                                                               AGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTT                                        >  SRR19973071.2997543/1‑150 (MQ=60)
                                                                                                               AGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTT                                        >  SRR19973071.4463001/1‑150 (MQ=60)
                                                                                                               AGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTT                                        >  SRR19973071.4463213/1‑150 (MQ=60)
                                                                                                               AGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTT                                        >  SRR19973071.3950052/1‑150 (MQ=60)
                                                                                                                  ATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCCGGCAACGGTTGGC                                     >  SRR19973071.4178342/1‑150 (MQ=60)
                                                                                                                     TTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTG                                  >  SRR19973071.2056844/1‑150 (MQ=60)
                                                                                                                         TGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTA                              >  SRR19973071.3983113/1‑150 (MQ=60)
                                                                                                                         TGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTA                              >  SRR19973071.1744191/1‑150 (MQ=60)
                                                                                                                         TGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTA                              >  SRR19973071.1744087/1‑150 (MQ=60)
                                                                                                                           AGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACA                            >  SRR19973071.388760/1‑150 (MQ=60)
                                                                                                                           AGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACA                            >  SRR19973071.388779/1‑150 (MQ=60)
                                                                                                                               CTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTG                        >  SRR19973071.4064169/1‑150 (MQ=60)
                                                                                                                               CTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTG                        >  SRR19973071.4064102/1‑150 (MQ=60)
                                                                                                                               CTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTG                        >  SRR19973071.4746903/1‑150 (MQ=60)
                                                                                                                                    AACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATG                   >  SRR19973071.740407/1‑150 (MQ=60)
                                                                                                                                     ACAGCTTCTCGCTGTGTGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGG                  <  SRR19973071.2309513/150‑1 (MQ=60)
                                                                                                                                     ACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGG                  >  SRR19973071.1038957/1‑150 (MQ=60)
                                                                                                                                      CAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGC                 >  SRR19973071.1510354/1‑150 (MQ=60)
                                                                                                                                      CAGCTTCGCGCTGAGTGGGAAAATCGTCTATAACACCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGC                 <  SRR19973071.4568413/150‑1 (MQ=60)
                                                                                                                                       AGGTTCTCGGTGAGTGGTAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCG                <  SRR19973071.3064491/150‑1 (MQ=60)
                                                                                                                                       AGCTCCGCGCTGAGTGGTAAAAGCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCG                <  SRR19973071.1777231/150‑1 (MQ=60)
                                                                                                                                            CTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGA           >  SRR19973071.1453996/1‑150 (MQ=60)
                                                                                                                                            CTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGA           >  SRR19973071.3851737/1‑150 (MQ=60)
                                                                                                                                             TCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAA          >  SRR19973071.2475844/1‑150 (MQ=60)
                                                                                                                                             TAGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCACGGTCTTCAAGCCAATTGGCGTGCATGAATATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAA          >  SRR19973071.2475797/1‑150 (MQ=60)
                                                                                                                                                CTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTG       >  SRR19973071.4785425/1‑150 (MQ=60)
                                                                                                                                                 TGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGG      >  SRR19973071.3979811/1‑150 (MQ=60)
                                                                                                                                                 TGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTGTACAGGTGCCATGGCGAAAGAAGTGG      >  SRR19973071.1430727/1‑150 (MQ=60)
                                                                                                                                                  GAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGT     >  SRR19973071.2597447/1‑150 (MQ=60)
                                                                                                                                                     AGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGT  >  SRR19973071.4255285/1‑150 (MQ=60)
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GTCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGT  >  CP009273/3535221‑3535519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑