Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,070,543 G→T 100% D57Y (GAT→TAT)  dtd → D‑tyr‑tRNA(Tyr) deacylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,070,5430GT95.9% 595.7 / 13.7 195D57Y (GAT→TAT) dtdD‑tyr‑tRNA(Tyr) deacylase
Reads supporting (aligned to +/- strand):  ref base G (2/6);  new base T (82/105);  total (84/111)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 195 total aligned reads displayed.

AACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGT  >  CP009273/4070398‑4070687
                                                                                                                                                 |                                                                                                                                                
aaCCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTAt                                                                                                                                                >  2:1358626/1‑148 (MQ=255)
 aCCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATg                                                                                                                                               <  1:2137057/148‑1 (MQ=255)
     gTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGa                                                                                                                                           <  1:3138775/148‑1 (MQ=255)
     gTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGa                                                                                                                                           <  1:3166224/148‑1 (MQ=255)
      tGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGaa                                                                                                                                          <  1:2794815/148‑1 (MQ=255)
       gCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAg                                                                                                                                         <  1:2089594/148‑1 (MQ=255)
            cGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAg                                                                                                                                    >  1:1681174/1‑148 (MQ=255)
            cGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAg                                                                                                                                    >  1:540897/1‑148 (MQ=255)
               cACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATg                                                                                                                                 >  2:3538672/1‑148 (MQ=255)
               cACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATg                                                                                                                                 <  1:1558493/148‑1 (MQ=255)
                aCCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGa                                                                                                                                <  1:209233/148‑1 (MQ=255)
                aCCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGa                                                                                                                                <  2:2663119/148‑1 (MQ=255)
                aCCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGa                                                                                                                                >  1:1994209/1‑148 (MQ=255)
                aCCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGa                                                                                                                                <  2:467642/148‑1 (MQ=255)
                 ccGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGaa                                                                                                                               <  1:1181619/148‑1 (MQ=255)
                  cGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAAt                                                                                                                              >  2:1605744/1‑148 (MQ=255)
                    tGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAAtct                                                                                                                            >  2:1138533/1‑148 (MQ=255)
                    tGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAAtct                                                                                                                            >  1:455607/1‑148 (MQ=255)
                     ggagggagAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAAtctc                                                                                                                           <  1:2589289/148‑1 (MQ=255)
                         ggagAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACg                                                                                                                       <  1:2269283/148‑1 (MQ=255)
                                tGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACa                                                                                                                >  1:1537733/1‑148 (MQ=255)
                                tGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACa                                                                                                                <  1:1267788/148‑1 (MQ=255)
                                tGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACa                                                                                                                >  2:1913142/1‑148 (MQ=255)
                                tGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACa                                                                                                                >  1:2694144/1‑148 (MQ=255)
                                 gACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAg                                                                                                               <  2:1951359/148‑1 (MQ=255)
                                         aaaTTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGgcgggcgg                                                                                                       >  1:3276170/1‑148 (MQ=255)
                                         aaaTTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGgcgggcgg                                                                                                       <  2:1348313/148‑1 (MQ=255)
                                            ttGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAg                                                                                                    >  2:834941/1‑148 (MQ=255)
                                             tGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAgt                                                                                                   <  2:3357992/148‑1 (MQ=255)
                                                 gcgGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGc                                                                                               <  2:2922553/148‑1 (MQ=255)
                                                 gcgGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGc                                                                                               <  1:1474091/148‑1 (MQ=255)
                                                  cgGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCt                                                                                              >  1:2603564/1‑148 (MQ=255)
                                                     gACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCtggt                                                                                           >  2:3419322/1‑148 (MQ=255)
                                                     gACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCtggt                                                                                           <  1:1605744/148‑1 (MQ=255)
                                                     gACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCtggt                                                                                           >  1:134412/1‑148 (MQ=255)
                                                     gACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCtggt                                                                                           >  1:2214658/1‑148 (MQ=255)
                                                     gACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCtggt                                                                                           >  1:131960/1‑148 (MQ=255)
                                                       cTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCtggtgg                                                                                         <  2:2949083/148‑1 (MQ=255)
                                                        ttttGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTggtggt                                                                                        <  1:1358626/148‑1 (MQ=255)
                                                        ttttGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTggtggt                                                                                        >  1:1985014/1‑148 (MQ=255)
                                                         tttGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGtt                                                                                       >  1:3491391/1‑148 (MQ=255)
                                                          ttGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGttt                                                                                      <  2:1764816/148‑1 (MQ=255)
                                                            ggTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTcc                                                                                    <  1:816495/148‑1 (MQ=255)
                                                            ggTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTcc                                                                                    <  2:368044/148‑1 (MQ=255)
                                                            ggTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTcc                                                                                    <  2:369367/148‑1 (MQ=255)
                                                             gtgtTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTccc                                                                                   <  2:2694144/148‑1 (MQ=255)
                                                               gtTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAg                                                                                 <  1:696937/148‑1 (MQ=255)
                                                                ttATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGt                                                                                >  2:1575157/1‑148 (MQ=255)
                                                                ttATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGt                                                                                >  2:3334603/1‑148 (MQ=255)
                                                                   ttGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTa                                                                             <  1:1054080/148‑1 (MQ=255)
                                                                   ttGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTa                                                                             <  2:2572663/148‑1 (MQ=255)
                                                                   ttGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTa                                                                             <  1:2828790/148‑1 (MQ=255)
                                                                       gtgtCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCt                                                                         <  1:1837897/148‑1 (MQ=255)
                                                                         gtCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCACCAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTcg                                                                       >  2:540846/1‑148 (MQ=255)
                                                                              aaaGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCa                                                                  >  1:2380509/1‑148 (MQ=255)
                                                                                aGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGa                                                                >  1:1111209/1‑148 (MQ=255)
                                                                                 ggATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGAt                                                               <  1:592138/148‑1 (MQ=255)
                                                                                 ggATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGAt                                                               <  1:2465781/148‑1 (MQ=255)
                                                                                 ggATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGAt                                                               >  2:1018683/1‑148 (MQ=255)
                                                                                      acgacgAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGa                                                          <  2:2175082/148‑1 (MQ=255)
                                                                                        gacgaACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAAc                                                        <  2:3211497/148‑1 (MQ=255)
                                                                                            aaCAGAAAGCAAACCGTCTGTGCGGGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACgggg                                                    >  2:615460/1‑148 (MQ=255)
                                                                                            aaCAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACgggg                                                    <  2:1342215/148‑1 (MQ=255)
                                                                                            aaCAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACgggg                                                    >  2:1428737/1‑148 (MQ=255)
                                                                                             aCAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACggggg                                                   <  1:289431/148‑1 (MQ=255)
                                                                                             aCAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACggggg                                                   <  2:1693213/148‑1 (MQ=255)
                                                                                              cAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGa                                                  <  1:2904472/148‑1 (MQ=255)
                                                                                              cAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGa                                                  <  2:282231/148‑1 (MQ=255)
                                                                                               aGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGAt                                                 <  1:3327030/148‑1 (MQ=255)
                                                                                                   aGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATgcgc                                             >  1:746487/1‑148 (MQ=255)
                                                                                                     cAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCGGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGccc                                           >  1:481089/1‑148 (MQ=255)
                                                                                                         ccGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGt                                       <  2:174628/148‑1 (MQ=255)
                                                                                                         ccGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGt                                       >  1:863987/1‑148 (MQ=255)
                                                                                                         ccGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGt                                       <  1:296247/148‑1 (MQ=255)
                                                                                                           gTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGttt                                     >  2:2105236/1‑148 (MQ=255)
                                                                                                           gTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGttt                                     <  1:485723/148‑1 (MQ=255)
                                                                                                           gTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGttt                                     <  1:2654451/148‑1 (MQ=255)
                                                                                                            tCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTtc                                    >  1:1734812/1‑148 (MQ=255)
                                                                                                              tgtgCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTtctc                                  <  2:791201/148‑1 (MQ=255)
                                                                                                              tgtgCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTtctc                                  <  2:2922456/148‑1 (MQ=255)
                                                                                                                  cGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCaaa                              <  1:304302/148‑1 (MQ=255)
                                                                                                                  cGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCaaa                              >  1:2551590/1‑148 (MQ=255)
                                                                                                                  cGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCaaa                              <  1:2637641/148‑1 (MQ=255)
                                                                                                                        tgtgCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCa                        <  2:1511494/148‑1 (MQ=255)
                                                                                                                         gtgCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCAt                       >  2:2018499/1‑148 (MQ=255)
                                                                                                                           gCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCa                     <  2:2618237/148‑1 (MQ=255)
                                                                                                                             tCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCAcc                   >  2:1236801/1‑148 (MQ=255)
                                                                                                                              cGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCg                  >  2:2736214/1‑148 (MQ=255)
                                                                                                                               ggCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCTAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCgg                 >  2:2778302/1‑148 (MQ=255)
                                                                                                                               ggCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCgg                 <  1:1023922/148‑1 (MQ=255)
                                                                                                                                gCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGa                <  1:1736398/148‑1 (MQ=255)
                                                                                                                                  tACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATc              >  2:2337959/1‑148 (MQ=255)
                                                                                                                                  tACCGCATCTATAGCTATGCAGAAGGCAAGTTTTATATAAAAGATCAACAGGCTGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACACTCGCCGCAGATACCGAACGTGGTATGCGCAAAAGTTTCTCCAAAGGTGCAAAAACGGATc              >  2:3067206/1‑148 (MQ=255)
                                                                                                                                   aCCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATcg             <  2:2427779/148‑1 (MQ=255)
                                                                                                                                          cTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCagag      >  2:653124/1‑148 (MQ=255)
                                                                                                                                           tttAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAgg     >  2:1273664/1‑148 (MQ=255)
                                                                                                                                            ttAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGc    <  1:1509383/148‑1 (MQ=255)
                                                                                                                                             tAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCg   >  2:1496823/1‑148 (MQ=255)
                                                                                                                                              aGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGt  <  1:1018683/148‑1 (MQ=255)
                                                                                                                                              aGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGt  <  2:1994209/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGT  >  CP009273/4070398‑4070687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATAT  >  CP009273/4070397‑4070692
                                                                                                                                                  |                                                                                                                                                     
TAACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATG                                                                                                                                                    <  SRR19973067.1458479/150‑1 (MQ=60)
TAACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATG                                                                                                                                                    <  SRR19973067.2397753/150‑1 (MQ=60)
TAACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATG                                                                                                                                                    >  SRR19973067.634696/1‑150 (MQ=60)
  ACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCC                                                                                                                                                  <  SRR19973067.2151338/150‑1 (MQ=60)
      GTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAG                                                                                                                                              <  SRR19973067.3186273/150‑1 (MQ=60)
      GTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAG                                                                                                                                              <  SRR19973067.3158707/150‑1 (MQ=60)
       TGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGG                                                                                                                                             <  SRR19973067.2812846/150‑1 (MQ=60)
       TGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGG                                                                                                                                             <  SRR19973067.1699424/150‑1 (MQ=60)
        GCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGC                                                                                                                                            <  SRR19973067.2103421/150‑1 (MQ=60)
           AGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAG                                                                                                                                         >  SRR19973067.544494/1‑150 (MQ=60)
           AGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAG                                                                                                                                         <  SRR19973067.2677623/150‑1 (MQ=60)
           AGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAG                                                                                                                                         >  SRR19973067.258745/1‑150 (MQ=60)
           AGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAG                                                                                                                                         >  SRR19973067.1692762/1‑150 (MQ=60)
               TCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGA                                                                                                                                     >  SRR19973067.2007678/1‑150 (MQ=60)
                CACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAA                                                                                                                                    <  SRR19973067.1569122/150‑1 (MQ=60)
                CACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAA                                                                                                                                    <  SRR19973067.905098/150‑1 (MQ=60)
                CACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAA                                                                                                                                    >  SRR19973067.1488258/1‑150 (MQ=60)
                 ACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAAT                                                                                                                                   <  SRR19973067.210800/150‑1 (MQ=60)
                  CCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATC                                                                                                                                  <  SRR19973067.1242061/150‑1 (MQ=60)
                  CCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATC                                                                                                                                  <  SRR19973067.1189419/150‑1 (MQ=60)
                   CGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCT                                                                                                                                 >  SRR19973067.458761/1‑150 (MQ=60)
                    GTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTC                                                                                                                                >  SRR19973067.1186237/1‑150 (MQ=60)
                      GGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAA                                                                                                                              <  SRR19973067.2606144/150‑1 (MQ=60)
                         GGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGT                                                                                                                           >  SRR19973067.1036022/1‑150 (MQ=60)
                          GGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTG                                                                                                                          <  SRR19973067.2284318/150‑1 (MQ=60)
                          GGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTG                                                                                                                          <  SRR19973067.2284230/150‑1 (MQ=60)
                          GGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTG                                                                                                                          <  SRR19973067.2284393/150‑1 (MQ=60)
                            AGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCA                                                                                                                        >  SRR19973067.2635218/1‑150 (MQ=60)
                               AGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACA                                                                                                                     >  SRR19973067.2711470/1‑150 (MQ=60)
                               AGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACA                                                                                                                     >  SRR19973067.1548150/1‑150 (MQ=60)
                               AGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACA                                                                                                                     >  SRR19973067.1548145/1‑150 (MQ=60)
                                GTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAG                                                                                                                    <  SRR19973067.1420738/150‑1 (MQ=60)
                                 TGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGG                                                                                                                   <  SRR19973067.1276398/150‑1 (MQ=60)
                                        CGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGG                                                                                                            >  SRR19973067.3296679/1‑150 (MQ=60)
                                            ATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGT                                                                                                        >  SRR19973067.420506/1‑150 (MQ=60)
                                            ATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGT                                                                                                        >  SRR19973067.3491567/1‑150 (MQ=60)
                                                 CGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCT                                                                                                   >  SRR19973067.2620492/1‑150 (MQ=60)
                                                  GCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTG                                                                                                  <  SRR19973067.1483903/150‑1 (MQ=60)
                                                    GGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGT                                                                                                >  SRR19973067.2229406/1‑150 (MQ=60)
                                                    GGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGT                                                                                                >  SRR19973067.135616/1‑150 (MQ=60)
                                                    GGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGT                                                                                                >  SRR19973067.2614551/1‑150 (MQ=60)
                                                    GGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGT                                                                                                >  SRR19973067.133177/1‑150 (MQ=60)
                                                    GGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGT                                                                                                >  SRR19973067.133149/1‑150 (MQ=60)
                                                    GGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGT                                                                                                >  SRR19973067.562513/1‑150 (MQ=60)
                                                      GACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGG                                                                                              <  SRR19973067.1616763/150‑1 (MQ=60)
                                                      GACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGG                                                                                              <  SRR19973067.1616753/150‑1 (MQ=60)
                                                       ACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGT                                                                                             >  SRR19973067.1998448/1‑150 (MQ=60)
                                                        CTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTT                                                                                            >  SRR19973067.3513549/1‑150 (MQ=60)
                                                        CTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTT                                                                                            >  SRR19973067.3301822/1‑150 (MQ=60)
                                                         TTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTT                                                                                           <  SRR19973067.1367906/150‑1 (MQ=60)
                                                         TTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTT                                                                                           <  SRR19973067.1366725/150‑1 (MQ=60)
                                                             GGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCA                                                                                       <  SRR19973067.822090/150‑1 (MQ=60)
                                                             GGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCA                                                                                       <  SRR19973067.822078/150‑1 (MQ=60)
                                                              GTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAG                                                                                      >  SRR19973067.3166830/1‑150 (MQ=60)
                                                               TGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGT                                                                                     >  SRR19973067.2006577/1‑150 (MQ=60)
                                                                GTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTT                                                                                    <  SRR19973067.701751/150‑1 (MQ=60)
                                                                  TATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTA                                                                                  <  SRR19973067.2119349/150‑1 (MQ=60)
                                                                    TTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACC                                                                                <  SRR19973067.1061305/150‑1 (MQ=60)
                                                                    TTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACC                                                                                <  SRR19973067.2846977/150‑1 (MQ=60)
                                                                    TTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACC                                                                                <  SRR19973067.2846948/150‑1 (MQ=60)
                                                                      GGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCT                                                                              <  SRR19973067.1654590/150‑1 (MQ=60)
                                                                       GGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTC                                                                             >  SRR19973067.294220/1‑150 (MQ=60)
                                                                        GTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCG                                                                            <  SRR19973067.1850422/150‑1 (MQ=60)
                                                                        GTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCG                                                                            >  SRR19973067.1322071/1‑150 (MQ=60)
                                                                             GAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCA                                                                       >  SRR19973067.2395968/1‑150 (MQ=60)
                                                                               AAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGA                                                                     >  SRR19973067.1118744/1‑150 (MQ=60)
                                                                                  GGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATAC                                                                  <  SRR19973067.595901/150‑1 (MQ=60)
                                                                                  GGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATAC                                                                  <  SRR19973067.2481984/150‑1 (MQ=60)
                                                                                  GGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATAC                                                                  <  SRR19973067.2481999/150‑1 (MQ=60)
                                                                                  GGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATAC                                                                  <  SRR19973067.1544409/150‑1 (MQ=60)
                                                                                            GAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGG                                                        <  SRR19973067.254950/150‑1 (MQ=60)
                                                                                              ACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGAT                                                      <  SRR19973067.2202288/150‑1 (MQ=60)
                                                                                              ACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGAT                                                      <  SRR19973067.291305/150‑1 (MQ=60)
                                                                                              ACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGAT                                                      <  SRR19973067.290939/150‑1 (MQ=60)
                                                                                               CAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATG                                                     <  SRR19973067.2922324/150‑1 (MQ=60)
                                                                                               CAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATG                                                     <  SRR19973067.2922987/150‑1 (MQ=60)
                                                                                                AGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGC                                                    >  SRR19973067.726241/1‑150 (MQ=60)
                                                                                                AGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGC                                                    <  SRR19973067.3348077/150‑1 (MQ=60)
                                                                                                  AAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGC                                                  >  SRR19973067.751669/1‑150 (MQ=60)
                                                                                                    AGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCGGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCC                                                >  SRR19973067.484407/1‑150 (MQ=60)
                                                                                                    AGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCC                                                >  SRR19973067.484530/1‑150 (MQ=60)
                                                                                                        AACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGT                                            >  SRR19973067.869782/1‑150 (MQ=60)
                                                                                                         ACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTT                                           >  SRR19973067.3060207/1‑150 (MQ=60)
                                                                                                          CCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTT                                          <  SRR19973067.298139/150‑1 (MQ=60)
                                                                                                           CGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTC                                         >  SRR19973067.1746686/1‑150 (MQ=60)
                                                                                                           CGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTC                                         >  SRR19973067.1748950/1‑150 (MQ=60)
                                                                                                            GTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCT                                        <  SRR19973067.489065/150‑1 (MQ=60)
                                                                                                            GTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCT                                        <  SRR19973067.2671550/150‑1 (MQ=60)
                                                                                                            GTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCT                                        <  SRR19973067.2671483/150‑1 (MQ=60)
                                                                                                               TGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCA                                     <  SRR19973067.1926195/150‑1 (MQ=60)
                                                                                                                 TGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAA                                   >  SRR19973067.2568279/1‑150 (MQ=60)
                                                                                                                   CGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGG                                 <  SRR19973067.2654700/150‑1 (MQ=60)
                                                                                                                   CGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGG                                 <  SRR19973067.306260/150‑1 (MQ=60)
                                                                                                                       CGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCA                             <  SRR19973067.2812692/150‑1 (MQ=60)
                                                                                                                       CGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCA                             <  SRR19973067.2090263/150‑1 (MQ=60)
                                                                                                                                GGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGAT                    <  SRR19973067.1030962/150‑1 (MQ=60)
                                                                                                                                 GCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATC                   <  SRR19973067.1748274/150‑1 (MQ=60)
                                                                                                                                 GCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATC                   <  SRR19973067.1747628/150‑1 (MQ=60)
                                                                                                                                 GCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATC                   <  SRR19973067.828857/150‑1 (MQ=60)
                                                                                                                                    ACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCG                >  SRR19973067.312456/1‑150 (MQ=60)
                                                                                                                                      CGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCA              <  SRR19973067.1078447/150‑1 (MQ=60)
                                                                                                                                         ATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAG           <  SRR19973067.489484/150‑1 (MQ=60)
                                                                                                                                             TTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGT       <  SRR19973067.1519396/150‑1 (MQ=60)
                                                                                                                                              TAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTT      <  SRR19973067.483016/150‑1 (MQ=60)
                                                                                                                                               AGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTA     <  SRR19973067.1025678/150‑1 (MQ=60)
                                                                                                                                                GCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTAT    >  SRR19973067.56336/1‑150 (MQ=60)
                                                                                                                                                GCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTAT    >  SRR19973067.946763/1‑150 (MQ=60)
                                                                                                                                                  TATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATAT  >  SRR19973067.415875/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
TAACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATAT  >  CP009273/4070397‑4070692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑