Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,070,543 G→T 100% D57Y (GAT→TAT)  dtd → D‑tyr‑tRNA(Tyr) deacylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,070,5430GT96.5% 530.7 / 9.5 173D57Y (GAT→TAT) dtdD‑tyr‑tRNA(Tyr) deacylase
Reads supporting (aligned to +/- strand):  ref base G (4/2);  new base T (81/86);  total (85/88)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 173 total aligned reads displayed.

ACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGT  >  CP009273/4070399‑4070687
                                                                                                                                                |                                                                                                                                                
aCCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATg                                                                                                                                               <  2:759313/148‑1 (MQ=255)
   cGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCg                                                                                                                                            >  1:2760931/1‑148 (MQ=255)
    gTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGa                                                                                                                                           <  1:623496/148‑1 (MQ=255)
     tGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGaa                                                                                                                                          <  2:1392420/148‑1 (MQ=255)
     tGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGaa                                                                                                                                          <  2:1392437/148‑1 (MQ=255)
     tGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGaa                                                                                                                                          <  2:247877/148‑1 (MQ=255)
       ccAGCGTCACCGTGGAGGGAGAAGTGGCGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAgg                                                                                                                                        >  2:34736/1‑148 (MQ=255)
         aGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCa                                                                                                                                      <  1:3143331/148‑1 (MQ=255)
         aGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCa                                                                                                                                      <  2:2756321/148‑1 (MQ=255)
           cGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAg                                                                                                                                    <  1:194989/148‑1 (MQ=255)
           cGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAg                                                                                                                                    <  1:193913/148‑1 (MQ=255)
           cGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAg                                                                                                                                    <  1:2213733/148‑1 (MQ=255)
           cGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAg                                                                                                                                    >  2:32968/1‑148 (MQ=255)
           cGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAg                                                                                                                                    >  1:2653284/1‑148 (MQ=255)
                ccGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGaa                                                                                                                               <  2:1229091/148‑1 (MQ=255)
                  gTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAAtc                                                                                                                             <  1:1513754/148‑1 (MQ=255)
                   tGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAAtct                                                                                                                            <  2:2606915/148‑1 (MQ=255)
                   tGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAAtct                                                                                                                            <  2:2794802/148‑1 (MQ=255)
                     gagggagAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCa                                                                                                                          <  2:625787/148‑1 (MQ=255)
                     gagggagAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCa                                                                                                                          <  1:103536/148‑1 (MQ=255)
                        ggagAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACg                                                                                                                       <  1:1191522/148‑1 (MQ=255)
                          agaAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTg                                                                                                                     >  2:2643367/1‑148 (MQ=255)
                          agaAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTg                                                                                                                     >  1:53685/1‑148 (MQ=255)
                            aaGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCa                                                                                                                   >  1:3007166/1‑148 (MQ=255)
                               tGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACa                                                                                                                <  2:2184136/148‑1 (MQ=255)
                                   gggCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAggcg                                                                                                            <  1:2254639/148‑1 (MQ=255)
                                     gCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAggcggg                                                                                                          <  2:1129916/148‑1 (MQ=255)
                                          aTTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCa                                                                                                     >  2:3054816/1‑148 (MQ=255)
                                          aTTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCa                                                                                                     >  2:1584712/1‑148 (MQ=255)
                                            tGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAgt                                                                                                   <  1:3055614/148‑1 (MQ=255)
                                            tGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAgt                                                                                                   <  2:2267113/148‑1 (MQ=255)
                                            tGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAgt                                                                                                   <  1:2400539/148‑1 (MQ=255)
                                                   ggACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCtgg                                                                                            >  2:1118946/1‑148 (MQ=255)
                                                   ggACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCtgg                                                                                            >  2:3026500/1‑148 (MQ=255)
                                                        tttGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGtt                                                                                       >  2:1028906/1‑148 (MQ=255)
                                                        tttGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGtt                                                                                       >  1:583589/1‑148 (MQ=255)
                                                         ttGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGttt                                                                                      <  2:2962310/148‑1 (MQ=255)
                                                           ggTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTcc                                                                                    <  2:1487647/148‑1 (MQ=255)
                                                            gtgtTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTccc                                                                                   >  1:3515901/1‑148 (MQ=255)
                                                             tgtTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCa                                                                                  >  1:396498/1‑148 (MQ=255)
                                                             tgtTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCa                                                                                  >  2:2113239/1‑148 (MQ=255)
                                                             tgtTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCa                                                                                  <  2:3581193/148‑1 (MQ=255)
                                                               ttATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGt                                                                                >  2:1918698/1‑148 (MQ=255)
                                                               ttATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGt                                                                                >  2:2128183/1‑148 (MQ=255)
                                                                tATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGtt                                                                               >  2:2448891/1‑148 (MQ=255)
                                                                tATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGtt                                                                               >  2:2967765/1‑148 (MQ=255)
                                                                   tGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTAc                                                                            >  2:3246753/1‑148 (MQ=255)
                                                                    gggTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTAcc                                                                           <  2:472851/148‑1 (MQ=255)
                                                                    gggTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTAcc                                                                           <  2:424376/148‑1 (MQ=255)
                                                                    gggTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTAcc                                                                           <  2:424265/148‑1 (MQ=255)
                                                                     ggTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTAccc                                                                          >  2:2855235/1‑148 (MQ=255)
                                                                     ggTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTAccc                                                                          >  2:2855162/1‑148 (MQ=255)
                                                                     ggTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGCGCTGGTGGTTTCCCAGTTTAccc                                                                          >  2:2855649/1‑148 (MQ=255)
                                                                         tCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTcgc                                                                      >  1:77341/1‑148 (MQ=255)
                                                                         tCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTcgc                                                                      >  1:2005234/1‑148 (MQ=255)
                                                                          cGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTcgcc                                                                     >  1:2277271/1‑148 (MQ=255)
                                                                          cGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTcgcc                                                                     >  1:2277145/1‑148 (MQ=255)
                                                                              aaGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAg                                                                 >  2:1342838/1‑148 (MQ=255)
                                                                                ggATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGAt                                                               >  2:1090500/1‑148 (MQ=255)
                                                                                ggATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGAt                                                               >  1:3680134/1‑148 (MQ=255)
                                                                                  aTGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATAc                                                             <  1:3786089/148‑1 (MQ=255)
                                                                                    gacgacGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCg                                                           >  1:2548495/1‑148 (MQ=255)
                                                                                     acgacgAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGa                                                          >  1:176222/1‑148 (MQ=255)
                                                                                     acgacgAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGa                                                          <  1:3617073/148‑1 (MQ=255)
                                                                                       gacgaACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAAc                                                        <  1:2823640/148‑1 (MQ=255)
                                                                                        acgaACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACg                                                       >  1:2623317/1‑148 (MQ=255)
                                                                                        acgaACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACg                                                       <  1:1980891/148‑1 (MQ=255)
                                                                                         cgaACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACgg                                                      <  1:1578173/148‑1 (MQ=255)
                                                                                           aaCAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACgggg                                                    >  2:103787/1‑148 (MQ=255)
                                                                                           aaCAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACgggg                                                    >  1:813874/1‑148 (MQ=255)
                                                                                           aaCAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACgggg                                                    >  1:813880/1‑148 (MQ=255)
                                                                                           aaCAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACgggg                                                    >  2:103786/1‑148 (MQ=255)
                                                                                            aCAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACggggg                                                   <  2:1952909/148‑1 (MQ=255)
                                                                                            aCAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACggggg                                                   <  2:2947410/148‑1 (MQ=255)
                                                                                               gAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATg                                                <  2:3499462/148‑1 (MQ=255)
                                                                                                  aGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATgcgc                                             >  2:508402/1‑148 (MQ=255)
                                                                                                      aaCCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCaa                                         >  2:914501/1‑148 (MQ=255)
                                                                                                      aaCCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCaa                                         >  1:1089980/1‑148 (MQ=255)
                                                                                                      aaCCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCaa                                         >  2:1868482/1‑148 (MQ=255)
                                                                                                      aaCCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCaa                                         >  1:598720/1‑148 (MQ=255)
                                                                                                       aCCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAg                                        >  2:986407/1‑148 (MQ=255)
                                                                                                       aCCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAg                                        <  2:827103/148‑1 (MQ=255)
                                                                                                       aCCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAg                                        >  1:3581618/1‑148 (MQ=255)
                                                                                                        ccGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGt                                       <  2:2801731/148‑1 (MQ=255)
                                                                                                        ccGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGt                                       <  2:888024/148‑1 (MQ=255)
                                                                                                        ccGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGt                                       <  2:562976/148‑1 (MQ=255)
                                                                                                        ccGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGt                                       <  2:562085/148‑1 (MQ=255)
                                                                                                                gCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCaa                               <  1:2444548/148‑1 (MQ=255)
                                                                                                                  gAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAg                             <  1:3066258/148‑1 (MQ=255)
                                                                                                                      gtgtGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGc                         <  1:3443095/148‑1 (MQ=255)
                                                                                                                          gCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCa                     <  1:3359452/148‑1 (MQ=255)
                                                                                                                          gCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCa                     <  1:3359402/148‑1 (MQ=255)
                                                                                                                           cTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCAc                    >  1:2846940/1‑148 (MQ=255)
                                                                                                                             cGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCg                  >  2:3736093/1‑148 (MQ=255)
                                                                                                                               gCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGa                <  1:97961/148‑1 (MQ=255)
                                                                                                                                    cGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATcgcg           >  1:1643420/1‑148 (MQ=255)
                                                                                                                                        tCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCaga       >  1:2100/1‑148 (MQ=255)
                                                                                                                                        tCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCaga       >  2:3611281/1‑148 (MQ=255)
                                                                                                                                         cTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCagag      >  1:2837875/1‑148 (MQ=255)
                                                                                                                                             aGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGt  <  1:34736/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGT  >  CP009273/4070399‑4070687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGTAACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATAT  >  CP009273/4070394‑4070692
                                                                                                                                                     |                                                                                                                                                     
GCGTAACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCT                                                                                                                                                       <  SRR19973069.975860/150‑1 (MQ=60)
   TAACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATG                                                                                                                                                    <  SRR19973069.338682/150‑1 (MQ=60)
     ACCCGTGCCAGCGTCACCTTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTTGTGTTATTGGGTTTCGAAAAGGATTACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCC                                                                                                                                                  <  SRR19973069.556191/150‑1 (MQ=60)
      CCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCG                                                                                                                                                 >  SRR19973069.2779356/1‑150 (MQ=60)
      CCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCG                                                                                                                                                 >  SRR19973069.2779352/1‑150 (MQ=60)
         GTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAG                                                                                                                                              <  SRR19973069.627638/150‑1 (MQ=60)
              AGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAG                                                                                                                                         <  SRR19973069.3163899/150‑1 (MQ=60)
              AGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAG                                                                                                                                         >  SRR19973069.2671062/1‑150 (MQ=60)
                CGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGAT                                                                                                                                       <  SRR19973069.2228729/150‑1 (MQ=60)
                CGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGAT                                                                                                                                       <  SRR19973069.195486/150‑1 (MQ=60)
                CGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGAT                                                                                                                                       <  SRR19973069.196567/150‑1 (MQ=60)
                 GTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATG                                                                                                                                      >  SRR19973069.2880673/1‑150 (MQ=60)
                      CGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCT                                                                                                                                 <  SRR19973069.3148843/150‑1 (MQ=60)
                       GTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTC                                                                                                                                <  SRR19973069.1524208/150‑1 (MQ=60)
                          GAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAAC                                                                                                                             <  SRR19973069.104565/150‑1 (MQ=60)
                           AGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCACGATGAATCTCAACG                                                                                                                            >  SRR19973069.1966422/1‑150 (MQ=60)
                             GGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTG                                                                                                                          <  SRR19973069.1617600/150‑1 (MQ=60)
                             GGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTG                                                                                                                          >  SRR19973069.54352/1‑150 (MQ=60)
                             GGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTG                                                                                                                          <  SRR19973069.1199736/150‑1 (MQ=60)
                             GGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTG                                                                                                                          <  SRR19973069.2231903/150‑1 (MQ=60)
                               AGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCA                                                                                                                        >  SRR19973069.3027118/1‑150 (MQ=60)
                                    TGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAATATGAATCTCAACGTGCAACAGG                                                                                                                   <  SRR19973069.1150392/150‑1 (MQ=60)
                                        GGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGG                                                                                                               <  SRR19973069.2269867/150‑1 (MQ=60)
                                                 TGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGT                                                                                                      <  SRR19973069.2418117/150‑1 (MQ=60)
                                                 TGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGT                                                                                                      <  SRR19973069.3075830/150‑1 (MQ=60)
                                                 TGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGT                                                                                                      <  SRR19973069.2416789/150‑1 (MQ=60)
                                                         GACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGG                                                                                              >  SRR19973069.3647950/1‑150 (MQ=60)
                                                           CTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTT                                                                                            >  SRR19973069.587584/1‑150 (MQ=60)
                                                           CTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTT                                                                                            >  SRR19973069.3510903/1‑150 (MQ=60)
                                                               TGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCC                                                                                        >  SRR19973069.3538585/1‑150 (MQ=60)
                                                                GGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCA                                                                                       >  SRR19973069.399404/1‑150 (MQ=60)
                                                                         GGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCT                                                                              <  SRR19973069.3809651/150‑1 (MQ=60)
                                                                            TGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGC                                                                           >  SRR19973069.78189/1‑150 (MQ=60)
                                                                            TGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGC                                                                           >  SRR19973069.2019198/1‑150 (MQ=60)
                                                                             GTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCC                                                                          >  SRR19973069.2292600/1‑150 (MQ=60)
                                                                             GTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCC                                                                          >  SRR19973069.2292728/1‑150 (MQ=60)
                                                                                   AAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGAT                                                                    >  SRR19973069.3704207/1‑150 (MQ=60)
                                                                                    AGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATA                                                                   <  SRR19973069.2830444/150‑1 (MQ=60)
                                                                                     GGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATAC                                                                  <  SRR19973069.205781/150‑1 (MQ=60)
                                                                                      GATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACC                                                                 <  SRR19973069.620234/150‑1 (MQ=60)
                                                                                       ATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACTGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCG                                                                >  SRR19973069.2565006/1‑150 (MQ=60)
                                                                                       ATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCG                                                                >  SRR19973069.2565339/1‑150 (MQ=60)
                                                                                       ATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCG                                                                <  SRR19973069.3810653/150‑1 (MQ=60)
                                                                                        TGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGA                                                               >  SRR19973069.177711/1‑150 (MQ=60)
                                                                                          ACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAAC                                                             <  SRR19973069.3640697/150‑1 (MQ=60)
                                                                                           CGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACG                                                            >  SRR19973069.2640865/1‑150 (MQ=60)
                                                                                            GACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGG                                                           <  SRR19973069.2842314/150‑1 (MQ=60)
                                                                                             ACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGG                                                          <  SRR19973069.1994666/150‑1 (MQ=60)
                                                                                             ACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGG                                                          <  SRR19973069.1994721/150‑1 (MQ=60)
                                                                                              CGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGG                                                         <  SRR19973069.1588891/150‑1 (MQ=60)
                                                                                              CGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGG                                                         >  SRR19973069.819627/1‑150 (MQ=60)
                                                                                              CGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGG                                                         >  SRR19973069.819621/1‑150 (MQ=60)
                                                                                              CGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGG                                                         >  SRR19973069.819117/1‑150 (MQ=60)
                                                                                              CGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGG                                                         >  SRR19973069.2290684/1‑150 (MQ=60)
                                                                                                   AGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGC                                                    >  SRR19973069.2327019/1‑150 (MQ=60)
                                                                                                         CAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAA                                              >  SRR19973069.1097591/1‑150 (MQ=60)
                                                                                                         CAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAA                                              >  SRR19973069.602759/1‑150 (MQ=60)
                                                                                                          AAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAG                                             >  SRR19973069.3604973/1‑150 (MQ=60)
                                                                                                          AAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAG                                             >  SRR19973069.3605667/1‑150 (MQ=60)
                                                                                                           AACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGT                                            <  SRR19973069.1294709/150‑1 (MQ=60)
                                                                                                            ACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTT                                           >  SRR19973069.983055/1‑150 (MQ=60)
                                                                                                             CCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTT                                          <  SRR19973069.1710736/150‑1 (MQ=60)
                                                                                                               GTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCT                                        >  SRR19973069.3270887/1‑150 (MQ=60)
                                                                                                                     GCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAG                                  <  SRR19973069.2461005/150‑1 (MQ=60)
                                                                                                                     GCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAG                                  <  SRR19973069.529627/150‑1 (MQ=60)
                                                                                                                       GAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGT                                <  SRR19973069.3086527/150‑1 (MQ=60)
                                                                                                                       GAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGT                                <  SRR19973069.3086480/150‑1 (MQ=60)
                                                                                                                         GCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGC                              <  SRR19973069.1870773/150‑1 (MQ=60)
                                                                                                                           GTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCAT                            <  SRR19973069.3465443/150‑1 (MQ=60)
                                                                                                                              TGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCAC                         >  SRR19973069.2865686/1‑150 (MQ=60)
                                                                                                                              TGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCAC                         >  SRR19973069.2865703/1‑150 (MQ=60)
                                                                                                                               GCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACC                        <  SRR19973069.3381788/150‑1 (MQ=60)
                                                                                                                               GCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACC                        <  SRR19973069.3381434/150‑1 (MQ=60)
                                                                                                                               GCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACC                        <  SRR19973069.3381384/150‑1 (MQ=60)
                                                                                                                                 TCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGG                      >  SRR19973069.77826/1‑150 (MQ=60)
                                                                                                                                    GCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATC                   <  SRR19973069.99207/150‑1 (MQ=60)
                                                                                                                                    GCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATC                   <  SRR19973069.98950/150‑1 (MQ=60)
                                                                                                                                       ACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCG                >  SRR19973069.2643257/1‑150 (MQ=60)
                                                                                                                                       ACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCG                >  SRR19973069.1654723/1‑150 (MQ=60)
                                                                                                                                           CATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGA            >  SRR19973069.2132/1‑150 (MQ=60)
                                                                                                                                            ATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAG           >  SRR19973069.2856608/1‑150 (MQ=60)
                                                                                                                                                 TAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTT      >  SRR19973069.847998/1‑150 (MQ=60)
                                                                                                                                                  AGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTA     <  SRR19973069.35221/150‑1 (MQ=60)
                                                                                                                                                  AGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTA     <  SRR19973069.35010/150‑1 (MQ=60)
                                                                                                                                                   GCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTAT    <  SRR19973069.2012917/150‑1 (MQ=60)
                                                                                                                                                   GCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTAT    <  SRR19973069.1642447/150‑1 (MQ=60)
                                                                                                                                                   GCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTAT    <  SRR19973069.2014125/150‑1 (MQ=60)
                                                                                                                                                   GCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTAT    <  SRR19973069.3678560/150‑1 (MQ=60)
                                                                                                                                                     TATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATAT  <  SRR19973069.3387848/150‑1 (MQ=60)
                                                                                                                                                     TATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATAT  <  SRR19973069.3388226/150‑1 (MQ=60)
                                                                                                                                                     TATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATAT  >  SRR19973069.3063853/1‑150 (MQ=60)
                                                                                                                                                     TATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATAT  >  SRR19973069.3063862/1‑150 (MQ=60)
                                                                                                                                                     TATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATAT  <  SRR19973069.3674942/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GCGTAACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATAT  >  CP009273/4070394‑4070692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑