Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,074,579 G→T 100% R4S (CGC→AGC)  fdhE ← formate dehydrogenase formation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,074,5790GT99.4% 591.2 / ‑4.2 181R4S (CGC→AGC) fdhEformate dehydrogenase formation protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (77/102);  total (78/103)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 181 total aligned reads displayed.

CACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGC  >  CP009273/4074435‑4074723
                                                                                                                                                |                                                                                                                                                
cACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAAt                                                                                                                                               <  1:2183595/148‑1 (MQ=255)
cACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAAt                                                                                                                                               <  1:3131540/148‑1 (MQ=255)
   ccAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACt                                                                                                                                            >  2:743488/1‑148 (MQ=255)
     agagGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCa                                                                                                                                          <  2:2037360/148‑1 (MQ=255)
     agagGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCa                                                                                                                                          <  2:2037847/148‑1 (MQ=255)
      gagGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCAt                                                                                                                                         >  1:1559352/1‑148 (MQ=255)
      gagGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCAt                                                                                                                                         >  1:1880865/1‑148 (MQ=255)
       agGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCAtt                                                                                                                                        <  2:2699688/148‑1 (MQ=255)
       agGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCAtt                                                                                                                                        <  2:583581/148‑1 (MQ=255)
        ggATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTc                                                                                                                                       <  2:1063056/148‑1 (MQ=255)
        ggATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTc                                                                                                                                       >  1:1023919/1‑148 (MQ=255)
          attattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAg                                                                                                                                     <  2:876417/148‑1 (MQ=255)
          attattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAg                                                                                                                                     <  2:3376488/148‑1 (MQ=255)
           ttattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGc                                                                                                                                    <  2:2242088/148‑1 (MQ=255)
           ttattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGc                                                                                                                                    >  1:2683429/1‑148 (MQ=255)
            tattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGct                                                                                                                                   <  2:3469718/148‑1 (MQ=255)
            tattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGct                                                                                                                                   <  2:3469794/148‑1 (MQ=255)
             attTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctt                                                                                                                                  <  2:1753244/148‑1 (MQ=255)
               tttCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttc                                                                                                                                >  1:752755/1‑148 (MQ=255)
               tttCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttc                                                                                                                                <  2:1485034/148‑1 (MQ=255)
                ttCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttct                                                                                                                               <  1:291766/148‑1 (MQ=255)
                 tCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttctt                                                                                                                              <  1:112267/148‑1 (MQ=255)
                 tCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttctt                                                                                                                              <  1:1910519/148‑1 (MQ=255)
                  cTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttcttt                                                                                                                             <  1:2270207/148‑1 (MQ=255)
                  cTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttcttt                                                                                                                             <  2:1340161/148‑1 (MQ=255)
                   tGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCtttt                                                                                                                            <  1:406565/148‑1 (MQ=255)
                   tGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCtttt                                                                                                                            <  1:406525/148‑1 (MQ=255)
                    gCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTc                                                                                                                           <  2:2396434/148‑1 (MQ=255)
                    gCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTc                                                                                                                           <  1:2943200/148‑1 (MQ=255)
                       aGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGt                                                                                                                        <  1:1350918/148‑1 (MQ=255)
                       aGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGt                                                                                                                        <  2:1092564/148‑1 (MQ=255)
                        gCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGtt                                                                                                                       >  1:1011275/1‑148 (MQ=255)
                         cTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTg                                                                                                                      >  1:3145198/1‑148 (MQ=255)
                           cgcgCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTc                                                                                                                    <  2:830868/148‑1 (MQ=255)
                             cgcAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCtt                                                                                                                  <  1:3026239/148‑1 (MQ=255)
                              gcAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTg                                                                                                                 <  1:3325040/148‑1 (MQ=255)
                                 gACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCgg                                                                                                              <  2:3211532/148‑1 (MQ=255)
                                        ggcgcggcgGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCAc                                                                                                       <  1:2084709/148‑1 (MQ=255)
                                         gcgcggcgGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACg                                                                                                      <  2:1661647/148‑1 (MQ=255)
                                            cggcggTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTa                                                                                                   <  1:1477119/148‑1 (MQ=255)
                                                  ttGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAgcg                                                                                             >  2:458647/1‑148 (MQ=255)
                                                  ttGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAgcg                                                                                             >  2:918203/1‑148 (MQ=255)
                                                     tataAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCgg                                                                                          >  2:739605/1‑148 (MQ=255)
                                                     tataAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCgg                                                                                          >  2:2521798/1‑148 (MQ=255)
                                                      ataAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCggg                                                                                         <  1:2255856/148‑1 (MQ=255)
                                                       taAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGt                                                                                        >  1:849275/1‑148 (MQ=255)
                                                        aaaTTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTg                                                                                       <  2:1087804/148‑1 (MQ=255)
                                                         aaTTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGa                                                                                      <  2:431952/148‑1 (MQ=255)
                                                         aaTTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGa                                                                                      <  1:721211/148‑1 (MQ=255)
                                                         aaTTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGa                                                                                      <  2:431595/148‑1 (MQ=255)
                                                          aTTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCTCGGGTGAt                                                                                     >  1:2234057/1‑148 (MQ=255)
                                                               tGAGCCGTGGGAACAATACAGGCGGAATCATATCCGCCGTACGTGTCTCGCTTGTACCCAGCTCATCTTGCGGTATTATGCTATTACACATTGAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCTGCGCGGGTGATGTttc                                                                                <  2:2619369/148‑1 (MQ=255)
                                                               tGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTttc                                                                                >  2:1353919/1‑148 (MQ=255)
                                                               tGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTttc                                                                                >  2:3122614/1‑148 (MQ=255)
                                                               tGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTttc                                                                                <  2:2619973/148‑1 (MQ=255)
                                                               tGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTttc                                                                                >  1:2424372/1‑148 (MQ=255)
                                                                gAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTttct                                                                               <  2:3049581/148‑1 (MQ=255)
                                                                gAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTttct                                                                               <  1:202096/148‑1 (MQ=255)
                                                                gAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTttct                                                                               <  1:202184/148‑1 (MQ=255)
                                                                  gCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTttcttc                                                                             >  1:15847/1‑148 (MQ=255)
                                                                  gCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTttcttc                                                                             >  1:1816783/1‑148 (MQ=255)
                                                                  gCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTttcttc                                                                             >  2:1592869/1‑148 (MQ=255)
                                                                   ccGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCg                                                                            >  2:2097111/1‑148 (MQ=255)
                                                                      aGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGccc                                                                         <  1:2485230/148‑1 (MQ=255)
                                                                      aGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGccc                                                                         >  1:2120545/1‑148 (MQ=255)
                                                                                caCGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGt                                                               <  1:345178/147‑1 (MQ=255)
                                                                                aaCGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGt                                                               >  2:979976/1‑148 (MQ=255)
                                                                                 aCGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTa                                                              >  1:1390972/1‑148 (MQ=255)
                                                                                 aCGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTa                                                              >  1:2495960/1‑148 (MQ=255)
                                                                                     cggAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAAccc                                                          >  2:604157/1‑148 (MQ=255)
                                                                                       gAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCAt                                                        >  2:556413/1‑148 (MQ=255)
                                                                                        aaTCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATc                                                       >  2:2899167/1‑148 (MQ=255)
                                                                                         aTCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATcc                                                      <  1:2196587/148‑1 (MQ=255)
                                                                                             tatCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTcc                                                  <  2:1791252/148‑1 (MQ=255)
                                                                                                ccgccgTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTccacc                                               >  2:401922/1‑148 (MQ=255)
                                                                                                       aCGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCgg                                        <  1:3457363/148‑1 (MQ=255)
                                                                                                       aCGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCgg                                        >  1:611180/1‑148 (MQ=255)
                                                                                                             ctcGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCg                                  <  1:981270/148‑1 (MQ=255)
                                                                                                              tcGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGt                                 >  1:468984/1‑148 (MQ=255)
                                                                                                               cGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTg                                <  1:2946793/148‑1 (MQ=255)
                                                                                                                 cTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGcc                              <  2:2819552/148‑1 (MQ=255)
                                                                                                                 cTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGcc                              >  1:1236831/1‑148 (MQ=255)
                                                                                                                 cTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGcc                              <  2:1274209/148‑1 (MQ=255)
                                                                                                                 cTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGcc                              <  2:456603/148‑1 (MQ=255)
                                                                                                                 cTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGcc                              >  1:3169743/1‑148 (MQ=255)
                                                                                                                  ttGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCt                             >  1:2506332/1‑148 (MQ=255)
                                                                                                                  ttGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCt                             >  1:2619058/1‑148 (MQ=255)
                                                                                                                     aaCCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTc                          >  1:668820/1‑148 (MQ=255)
                                                                                                                      aCCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCa                         >  1:935915/1‑148 (MQ=255)
                                                                                                                           gCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCaa                    >  2:1573922/1‑148 (MQ=255)
                                                                                                                            cTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTCATCGTGCCTTTCACCCaaa                   >  2:1940247/1‑148 (MQ=255)
                                                                                                                             tCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAg                  <  1:1014537/148‑1 (MQ=255)
                                                                                                                              cATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAgg                 >  1:2456523/1‑148 (MQ=255)
                                                                                                                               aTCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAggg                <  1:1356207/148‑1 (MQ=255)
                                                                                                                                        gATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGa       >  2:2856708/1‑148 (MQ=255)
                                                                                                                                          ttATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGata     <  2:1075001/148‑1 (MQ=255)
                                                                                                                                           tATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGatat    >  1:3497583/1‑148 (MQ=255)
                                                                                                                                           tATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGatat    >  1:1721465/1‑148 (MQ=255)
                                                                                                                                            aTGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATg   <  1:3535607/148‑1 (MQ=255)
                                                                                                                                             tGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGc  >  2:1284913/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGC  >  CP009273/4074435‑4074723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCA  >  CP009273/4074430‑4074727
                                                                                                                                                     |                                                                                                                                                    
GTTATCACCCATAGGTTTATTTTCTGACAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAATTTCTTGAGCCGAGGTAACAATAACGGCGGAATAATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCT                                                                                                                                                      <  SRR19973067.1019818/150‑1 (MQ=60)
GTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCT                                                                                                                                                      <  SRR19973067.3165094/150‑1 (MQ=60)
GTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCT                                                                                                                                                      <  SRR19973067.3165112/150‑1 (MQ=60)
  AATCACCCAGAGGTTTATTTTCTTCCATCTCGCTCAGACGCTCGGCGCTGCGTTTTTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAA                                                                                                                                                    <  SRR19973067.813237/150‑1 (MQ=60)
   ATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAAT                                                                                                                                                   <  SRR19973067.1642313/150‑1 (MQ=60)
   ATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAAT                                                                                                                                                   >  SRR19973067.3489248/1‑150 (MQ=60)
   ATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAAT                                                                                                                                                   >  SRR19973067.2045387/1‑150 (MQ=60)
     CACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATAC                                                                                                                                                 <  SRR19973067.2987289/150‑1 (MQ=60)
     CACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATAC                                                                                                                                                 <  SRR19973067.3151439/150‑1 (MQ=60)
     CACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATAC                                                                                                                                                 <  SRR19973067.3151507/150‑1 (MQ=60)
     CACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATAC                                                                                                                                                 <  SRR19973067.2198208/150‑1 (MQ=60)
     CACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATAC                                                                                                                                                 <  SRR19973067.2198192/150‑1 (MQ=60)
         CAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCAT                                                                                                                                             >  SRR19973067.1569989/1‑150 (MQ=60)
         CAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCAT                                                                                                                                             >  SRR19973067.1893698/1‑150 (MQ=60)
           GAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTC                                                                                                                                           >  SRR19973067.1030959/1‑150 (MQ=60)
             GGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCGGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAG                                                                                                                                         <  SRR19973067.775178/150‑1 (MQ=60)
              GATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGC                                                                                                                                        >  SRR19973067.2700624/1‑150 (MQ=60)
                 TATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTT                                                                                                                                     >  SRR19973067.755816/1‑150 (MQ=60)
                  ATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTC                                                                                                                                    >  SRR19973067.757981/1‑150 (MQ=60)
                   TTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCT                                                                                                                                   >  SRR19973067.1988941/1‑150 (MQ=60)
                     TTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTT                                                                                                                                 <  SRR19973067.293646/150‑1 (MQ=60)
                      TCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTT                                                                                                                                <  SRR19973067.113351/150‑1 (MQ=60)
                      TCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTT                                                                                                                                <  SRR19973067.112794/150‑1 (MQ=60)
                      TCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTT                                                                                                                                <  SRR19973067.1923550/150‑1 (MQ=60)
                       CTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTC                                                                                                                               <  SRR19973067.484091/150‑1 (MQ=60)
                       CTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTC                                                                                                                               <  SRR19973067.2285246/150‑1 (MQ=60)
                       CTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTC                                                                                                                               <  SRR19973067.2285247/150‑1 (MQ=60)
                        TGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCC                                                                                                                              <  SRR19973067.409397/150‑1 (MQ=60)
                        TGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCC                                                                                                                              <  SRR19973067.409394/150‑1 (MQ=60)
                        TGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCC                                                                                                                              <  SRR19973067.409356/150‑1 (MQ=60)
                         GCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCG                                                                                                                             <  SRR19973067.2962476/150‑1 (MQ=60)
                         GCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCG                                                                                                                             <  SRR19973067.2961848/150‑1 (MQ=60)
                           CAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTT                                                                                                                           >  SRR19973067.1018222/1‑150 (MQ=60)
                            AGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTG                                                                                                                          <  SRR19973067.1360151/150‑1 (MQ=60)
                            AGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTG                                                                                                                          >  SRR19973067.3165157/1‑150 (MQ=60)
                                  CGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGC                                                                                                                    <  SRR19973067.3045509/150‑1 (MQ=60)
                                   GCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCG                                                                                                                   <  SRR19973067.3346074/150‑1 (MQ=60)
                                       ACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACC                                                                                                               <  SRR19973067.2735394/150‑1 (MQ=60)
                                       ACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACC                                                                                                               <  SRR19973067.948689/150‑1 (MQ=60)
                                             GGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGG                                                                                                         <  SRR19973067.2098513/150‑1 (MQ=60)
                                                 CGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACC                                                                                                     <  SRR19973067.1486940/150‑1 (MQ=60)
                                                          TATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGT                                                                                            >  SRR19973067.855020/1‑150 (MQ=60)
                                                           ATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTG                                                                                           <  SRR19973067.2270827/150‑1 (MQ=60)
                                                             AAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCTCGGGTGAT                                                                                         >  SRR19973067.2248920/1‑150 (MQ=60)
                                                              AATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATG                                                                                        <  SRR19973067.726221/150‑1 (MQ=60)
                                                                  CTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTC                                                                                    >  SRR19973067.2440278/1‑150 (MQ=60)
                                                                     GAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTC                                                                                 >  SRR19973067.16094/1‑150 (MQ=60)
                                                                     GAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTC                                                                                 <  SRR19973067.203633/150‑1 (MQ=60)
                                                                     GAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTC                                                                                 <  SRR19973067.203721/150‑1 (MQ=60)
                                                                     GAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTC                                                                                 >  SRR19973067.1829085/1‑150 (MQ=60)
                                                                     GAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTC                                                                                 <  SRR19973067.3527387/150‑1 (MQ=60)
                                                                         CGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCC                                                                             >  SRR19973067.2134920/1‑150 (MQ=60)
                                                                         CGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCC                                                                             >  SRR19973067.2134689/1‑150 (MQ=60)
                                                                           AGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAG                                                                           <  SRR19973067.2501565/150‑1 (MQ=60)
                                                                            GGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGG                                                                          <  SRR19973067.2248893/150‑1 (MQ=60)
                                                                                    TAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTA                                                                  >  SRR19973067.2512353/1‑150 (MQ=60)
                                                                                    TAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTA                                                                  >  SRR19973067.1400425/1‑150 (MQ=60)
                                                                                     CACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAA                                                                 <  SRR19973067.347559/150‑1 (MQ=60)
                                                                                        GGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCC                                                              >  SRR19973067.1425779/1‑150 (MQ=60)
                                                                                           GGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATC                                                           >  SRR19973067.2110298/1‑150 (MQ=60)
                                                                                              ATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTT                                                        <  SRR19973067.2211243/150‑1 (MQ=60)
                                                                                                         CGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCG                                             >  SRR19973067.2380530/1‑150 (MQ=60)
                                                                                                          GTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGG                                            >  SRR19973067.615443/1‑150 (MQ=60)
                                                                                                          GTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGG                                            >  SRR19973067.615137/1‑150 (MQ=60)
                                                                                                            ACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTA                                          <  SRR19973067.3479347/150‑1 (MQ=60)
                                                                                                                TTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCG                                      <  SRR19973067.1255101/150‑1 (MQ=60)
                                                                                                                 TCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGT                                     >  SRR19973067.472226/1‑150 (MQ=60)
                                                                                                                  CTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTG                                    <  SRR19973067.988009/150‑1 (MQ=60)
                                                                                                                  CTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTG                                    <  SRR19973067.3479211/150‑1 (MQ=60)
                                                                                                                  CTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTG                                    >  SRR19973067.2678293/1‑150 (MQ=60)
                                                                                                                    CGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCC                                  >  SRR19973067.1244888/1‑150 (MQ=60)
                                                                                                                    CGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCC                                  >  SRR19973067.3189816/1‑150 (MQ=60)
                                                                                                                    CGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCC                                  <  SRR19973067.2965449/150‑1 (MQ=60)
                                                                                                                    CGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCC                                  >  SRR19973067.1245131/1‑150 (MQ=60)
                                                                                                                     GCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCT                                 >  SRR19973067.2636045/1‑150 (MQ=60)
                                                                                                                     GCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCT                                 >  SRR19973067.2522776/1‑150 (MQ=60)
                                                                                                                     GCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCT                                 >  SRR19973067.2522773/1‑150 (MQ=60)
                                                                                                                     GCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTATTCGCCCAGGCGCTGGTAACCCATCCTTCAACCATGGCGGTAATCGTGCCT                                 >  SRR19973067.2522818/1‑150 (MQ=60)
                                                                                                                      CTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTT                                <  SRR19973067.1571966/150‑1 (MQ=60)
                                                                                                                        TGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTC                              >  SRR19973067.1749556/1‑150 (MQ=60)
                                                                                                                        TGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTC                              >  SRR19973067.673397/1‑150 (MQ=60)
                                                                                                                         GAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCA                             >  SRR19973067.942268/1‑150 (MQ=60)
                                                                                                                          AACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCAC                            >  SRR19973067.1880297/1‑150 (MQ=60)
                                                                                                                            CCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCC                          <  SRR19973067.3384204/150‑1 (MQ=60)
                                                                                                                                GCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAG                      <  SRR19973067.924340/150‑1 (MQ=60)
                                                                                                                                GCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAG                      <  SRR19973067.461825/150‑1 (MQ=60)
                                                                                                                                 CTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGG                     >  SRR19973067.2472676/1‑150 (MQ=60)
                                                                                                                                 CTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGG                     >  SRR19973067.2472674/1‑150 (MQ=60)
                                                                                                                                  TCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGG                    <  SRR19973067.1021506/150‑1 (MQ=60)
                                                                                                                                  TCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGG                    <  SRR19973067.1021501/150‑1 (MQ=60)
                                                                                                                                   CATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGC                   >  SRR19973067.3288957/1‑150 (MQ=60)
                                                                                                                                    ATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCG                  <  SRR19973067.1366028/150‑1 (MQ=60)
                                                                                                                                    ATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCG                  <  SRR19973067.1365473/150‑1 (MQ=60)
                                                                                                                                              ATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATAT        <  SRR19973067.3136727/150‑1 (MQ=60)
                                                                                                                                              ATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATAT        >  SRR19973067.1733260/1‑150 (MQ=60)
                                                                                                                                              ATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATAT        >  SRR19973067.3519766/1‑150 (MQ=60)
                                                                                                                                               TTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATG       <  SRR19973067.189557/150‑1 (MQ=60)
                                                                                                                                                 ATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCA     <  SRR19973067.2494252/150‑1 (MQ=60)
                                                                                                                                                 ATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCA     <  SRR19973067.3557951/150‑1 (MQ=60)
                                                                                                                                                  TGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCAC    <  SRR19973067.154688/150‑1 (MQ=60)
                                                                                                                                                    CGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCA  >  SRR19973067.2599652/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCA  >  CP009273/4074430‑4074727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑