Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,074,579 G→T 100% R4S (CGC→AGC)  fdhE ← formate dehydrogenase formation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,074,5790GT99.0% 688.6 / ‑1.9 212R4S (CGC→AGC) fdhEformate dehydrogenase formation protein
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (88/121);  total (90/122)
Fisher's exact test for biased strand distribution p-value = 5.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 212 total aligned reads displayed.

TCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGC  >  CP009273/4074434‑4074723
                                                                                                                                                 |                                                                                                                                                
tCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTaa                                                                                                                                                >  1:3300272/1‑148 (MQ=255)
  aCCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATa                                                                                                                                              <  2:2800932/148‑1 (MQ=255)
  aCCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATa                                                                                                                                              <  2:3342109/148‑1 (MQ=255)
  aCCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATa                                                                                                                                              <  2:2801325/148‑1 (MQ=255)
  aCCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATa                                                                                                                                              <  2:3342120/148‑1 (MQ=255)
   cccAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATAc                                                                                                                                             >  2:2849568/1‑148 (MQ=255)
     cAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTc                                                                                                                                           <  2:1040851/148‑1 (MQ=255)
     cAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTc                                                                                                                                           <  2:1899582/148‑1 (MQ=255)
       gagGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCAt                                                                                                                                         <  1:232334/148‑1 (MQ=255)
        agGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCAtt                                                                                                                                        >  1:1675987/1‑148 (MQ=255)
         ggATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTc                                                                                                                                       <  2:561491/148‑1 (MQ=255)
          gATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCa                                                                                                                                      >  1:1291976/1‑148 (MQ=255)
           attattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAg                                                                                                                                     <  1:1881035/148‑1 (MQ=255)
            ttattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTTTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGc                                                                                                                                    <  1:1345338/148‑1 (MQ=255)
            ttattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGc                                                                                                                                    <  1:1571633/148‑1 (MQ=255)
            ttattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGc                                                                                                                                    >  1:1835889/1‑148 (MQ=255)
             tattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGct                                                                                                                                   >  1:1134349/1‑148 (MQ=255)
             tattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGct                                                                                                                                   <  1:2422805/148‑1 (MQ=255)
                tttCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttc                                                                                                                                <  1:400978/148‑1 (MQ=255)
                tttCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttc                                                                                                                                <  1:2533876/148‑1 (MQ=255)
                 ttCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttct                                                                                                                               <  2:276636/148‑1 (MQ=255)
                 ttCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttct                                                                                                                               <  1:2418410/148‑1 (MQ=255)
                  tCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttctt                                                                                                                              <  2:3347330/148‑1 (MQ=255)
                  tCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttctt                                                                                                                              <  2:3485710/148‑1 (MQ=255)
                        aGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGt                                                                                                                        <  2:59058/148‑1 (MQ=255)
                         gCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGACTCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGtt                                                                                                                       >  1:878428/1‑148 (MQ=255)
                          cTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTg                                                                                                                      >  2:178537/1‑148 (MQ=255)
                           tCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGt                                                                                                                     <  1:1423425/148‑1 (MQ=255)
                           tCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTACGTTGt                                                                                                                     >  2:1805024/1‑148 (MQ=255)
                            cgcgCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTc                                                                                                                    <  1:826028/148‑1 (MQ=255)
                             gcgcAGACGCTCGTCGCGGCGGTTGTATATATTCTTGAGCCGAGGGTACAATTACTGCGGAATCATATCCGCCGTACGTTTGTCGCTTGCACCCATCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCt                                                                                                                   <  2:891352/148‑1 (MQ=255)
                              cgcAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCtt                                                                                                                  >  1:1183622/1‑148 (MQ=255)
                               gcAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTg                                                                                                                 <  2:1675987/148‑1 (MQ=255)
                                   aCGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGa                                                                                                             >  1:3344821/1‑148 (MQ=255)
                                     gCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGTAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGAcc                                                                                                           <  1:1247978/148‑1 (MQ=255)
                                       tCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTc                                                                                                         <  2:3300272/148‑1 (MQ=255)
                                        cggcgcggcgGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCa                                                                                                        >  2:2964110/1‑148 (MQ=255)
                                           cgcggcgGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACgg                                                                                                     <  1:3371007/148‑1 (MQ=255)
                                            gcggcgGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGt                                                                                                    <  1:283538/148‑1 (MQ=255)
                                              ggcggTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTAc                                                                                                  <  1:2792748/148‑1 (MQ=255)
                                               gcggTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTc                                                                                                         >  2:2518200/1‑140 (MQ=255)
                                               gcggTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTAcc                                                                                                 <  2:512062/148‑1 (MQ=255)
                                                 ggTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAg                                                                                               <  1:1697690/148‑1 (MQ=255)
                                                        taAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGt                                                                                        <  1:428399/148‑1 (MQ=255)
                                                         aaaTTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTg                                                                                       <  2:2135964/148‑1 (MQ=255)
                                                         aaaTTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTg                                                                                       <  2:2135235/148‑1 (MQ=255)
                                                           aTTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGAt                                                                                     <  2:2783419/148‑1 (MQ=255)
                                                            ttCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATg                                                                                    <  2:1145948/148‑1 (MQ=255)
                                                             tCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGt                                                                                   <  1:2150985/148‑1 (MQ=255)
                                                             tCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGt                                                                                   >  1:2829758/1‑148 (MQ=255)
                                                                 gAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTttct                                                                               <  2:260255/148‑1 (MQ=255)
                                                                  aGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTttctt                                                                              <  1:2025731/148‑1 (MQ=255)
                                                                  aGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTttctt                                                                              <  1:2026719/148‑1 (MQ=255)
                                                                      gAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGcc                                                                          <  1:816305/148‑1 (MQ=255)
                                                                      gAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGcc                                                                          <  1:2876240/148‑1 (MQ=255)
                                                                       aGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGccc                                                                         >  1:714699/1‑148 (MQ=255)
                                                                       aGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGccc                                                                         >  2:2369024/1‑148 (MQ=255)
                                                                       aGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGccc                                                                         <  1:97019/148‑1 (MQ=255)
                                                                             cAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGgcgc                                                                   <  2:424012/148‑1 (MQ=255)
                                                                             cAAGAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGgcgc                                                                   <  2:2697377/148‑1 (MQ=255)
                                                                                tAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTgg                                                                >  1:3009633/1‑148 (MQ=255)
                                                                                tAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTgg                                                                >  2:668713/1‑148 (MQ=255)
                                                                                tAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTgg                                                                >  2:2371842/1‑148 (MQ=255)
                                                                                tAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTgg                                                                >  2:391340/1‑148 (MQ=255)
                                                                                tAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTgg                                                                >  1:1261218/1‑148 (MQ=255)
                                                                                   cggcggAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTaa                                                             >  2:2892318/1‑148 (MQ=255)
                                                                                        gAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCAt                                                        >  2:1837682/1‑148 (MQ=255)
                                                                                        gAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCAt                                                        >  2:2021225/1‑148 (MQ=255)
                                                                                         aaTCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATc                                                       <  1:432549/148‑1 (MQ=255)
                                                                                          aTCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATcc                                                      >  2:3077862/1‑148 (MQ=255)
                                                                                            cATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCtt                                                    >  2:271409/1‑148 (MQ=255)
                                                                                              tatCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTcc                                                  <  2:3420403/148‑1 (MQ=255)
                                                                                                tCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTAccac                                                >  1:3170458/1‑148 (MQ=255)
                                                                                                   gccgTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCAt                                             >  2:2832651/1‑148 (MQ=255)
                                                                                                       tACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCg                                         >  1:2458659/1‑148 (MQ=255)
                                                                                                            ttCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAAt                                    >  1:1101603/1‑148 (MQ=255)
                                                                                                            ttCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAAt                                    >  2:3176663/1‑148 (MQ=255)
                                                                                                              ctcGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCg                                  >  2:1490098/1‑148 (MQ=255)
                                                                                                              ctcGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCg                                  <  1:3030263/148‑1 (MQ=255)
                                                                                                                cGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTg                                >  2:9583/1‑148 (MQ=255)
                                                                                                                   ttGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCt                             <  2:2434995/148‑1 (MQ=255)
                                                                                                                     gAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCttt                           >  2:1247962/1‑148 (MQ=255)
                                                                                                                       aCCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCa                         >  1:1019350/1‑148 (MQ=255)
                                                                                                                       aCCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCa                         >  1:1531291/1‑148 (MQ=255)
                                                                                                                         ccAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCAcc                       <  1:1652543/148‑1 (MQ=255)
                                                                                                                           aGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCa                     >  1:370702/1‑148 (MQ=255)
                                                                                                                             cTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCaaa                   >  2:1004050/1‑148 (MQ=255)
                                                                                                                             cTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCaaa                   <  1:2731555/148‑1 (MQ=255)
                                                                                                                                      cGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGt          <  1:763745/148‑1 (MQ=255)
                                                                                                                                        ggATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAg        >  2:1586736/1‑148 (MQ=255)
                                                                                                                                        ggATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAg        <  2:1134349/148‑1 (MQ=255)
                                                                                                                                        ggATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAg        <  1:1797569/148‑1 (MQ=255)
                                                                                                                                         gATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGa       >  2:552861/1‑148 (MQ=255)
                                                                                                                                         gATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGa       <  2:3083637/148‑1 (MQ=255)
                                                                                                                                          aTTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGat      >  1:2697997/1‑148 (MQ=255)
                                                                                                                                          aTTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGat      >  2:3194035/1‑148 (MQ=255)
                                                                                                                                            tATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGatat    >  2:2259297/1‑148 (MQ=255)
                                                                                                                                             aTGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATg   >  2:2889577/1‑148 (MQ=255)
                                                                                                                                             aTGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATg   >  2:839567/1‑148 (MQ=255)
                                                                                                                                              tGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTGGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGc  <  1:1405106/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGC  >  CP009273/4074434‑4074723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCAT  >  CP009273/4074430‑4074728
                                                                                                                                                     |                                                                                                                                                     
GTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCT                                                                                                                                                       <  SRR19973065.179138/150‑1 (MQ=60)
GTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCT                                                                                                                                                       <  SRR19973065.1532314/150‑1 (MQ=60)
GTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCT                                                                                                                                                       <  SRR19973065.1474503/150‑1 (MQ=60)
GTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCT                                                                                                                                                       <  SRR19973065.1474485/150‑1 (MQ=60)
GTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCT                                                                                                                                                       <  SRR19973065.505653/150‑1 (MQ=60)
GTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCT                                                                                                                                                       <  SRR19973065.3285053/150‑1 (MQ=60)
 TAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTCTGCTA                                                                                                                                                      >  SRR19973065.2634584/1‑150 (MQ=60)
 TAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTA                                                                                                                                                      >  SRR19973065.2634590/1‑150 (MQ=60)
  AATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAA                                                                                                                                                     >  SRR19973065.3320373/1‑150 (MQ=60)
   ATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAAT                                                                                                                                                    <  SRR19973065.1904773/150‑1 (MQ=60)
         CAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCAT                                                                                                                                              <  SRR19973065.233469/150‑1 (MQ=60)
          AGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATT                                                                                                                                             >  SRR19973065.1687200/1‑150 (MQ=60)
          AGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATT                                                                                                                                             <  SRR19973065.1256747/150‑1 (MQ=60)
           GAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTC                                                                                                                                            <  SRR19973065.234023/150‑1 (MQ=60)
           GAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTC                                                                                                                                            >  SRR19973065.1706732/1‑150 (MQ=60)
            AGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCA                                                                                                                                           >  SRR19973065.1300488/1‑150 (MQ=60)
            AGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCA                                                                                                                                           >  SRR19973065.2091962/1‑150 (MQ=60)
              GATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGC                                                                                                                                         >  SRR19973065.59689/1‑150 (MQ=60)
              GATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGC                                                                                                                                         >  SRR19973065.1847996/1‑150 (MQ=60)
               ATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCT                                                                                                                                        >  SRR19973065.1142607/1‑150 (MQ=60)
               ATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCT                                                                                                                                        >  SRR19973065.1141701/1‑150 (MQ=60)
               ATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCT                                                                                                                                        <  SRR19973065.1893418/150‑1 (MQ=60)
                TTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTTTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTT                                                                                                                                       <  SRR19973065.1354235/150‑1 (MQ=60)
                TTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTT                                                                                                                                       <  SRR19973065.1110568/150‑1 (MQ=60)
                TTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTT                                                                                                                                       >  SRR19973065.1194650/1‑150 (MQ=60)
                TTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTT                                                                                                                                       <  SRR19973065.2872283/150‑1 (MQ=60)
                TTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTT                                                                                                                                       <  SRR19973065.1582088/150‑1 (MQ=60)
                TTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTT                                                                                                                                       <  SRR19973065.1669478/150‑1 (MQ=60)
                 TATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTT                                                                                                                                      >  SRR19973065.2440952/1‑150 (MQ=60)
                 TATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTT                                                                                                                                      <  SRR19973065.2438086/150‑1 (MQ=60)
                   TTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCT                                                                                                                                    >  SRR19973065.3061574/1‑150 (MQ=60)
                    TTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTT                                                                                                                                   <  SRR19973065.2549778/150‑1 (MQ=60)
                    TTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTT                                                                                                                                   <  SRR19973065.403788/150‑1 (MQ=60)
                    TTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTT                                                                                                                                   <  SRR19973065.850438/150‑1 (MQ=60)
                     TTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTT                                                                                                                                  <  SRR19973065.2433645/150‑1 (MQ=60)
                     TTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTT                                                                                                                                  <  SRR19973065.2433716/150‑1 (MQ=60)
                       CTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTC                                                                                                                                >  SRR19973065.1028845/1‑150 (MQ=60)
                       CTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTC                                                                                                                                >  SRR19973065.3036740/1‑150 (MQ=60)
                           CAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGACTCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCAGCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTT                                                                                                                            >  SRR19973065.884029/1‑150 (MQ=60)
                               TCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCT                                                                                                                        <  SRR19973065.1432702/150‑1 (MQ=60)
                               TCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCT                                                                                                                        <  SRR19973065.1432685/150‑1 (MQ=60)
                                CGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTT                                                                                                                       <  SRR19973065.996460/150‑1 (MQ=60)
                                CGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTT                                                                                                                       <  SRR19973065.831440/150‑1 (MQ=60)
                                CGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTT                                                                                                                       >  SRR19973065.1191116/1‑150 (MQ=60)
                                CGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTT                                                                                                                       <  SRR19973065.1762562/150‑1 (MQ=60)
                                     AGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGA                                                                                                                  >  SRR19973065.3365258/1‑150 (MQ=60)
                                     AGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGA                                                                                                                  >  SRR19973065.1403333/1‑150 (MQ=60)
                                        CGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCT                                                                                                               <  SRR19973065.2699463/150‑1 (MQ=60)
                                         GCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGTAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTC                                                                                                              <  SRR19973065.1256198/150‑1 (MQ=60)
                                           cgatctCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCAC                                                                                                            <  SRR19973065.2534013/144‑1 (MQ=60)
                                           TGGGCGCGGCGGTTGTATAAATTCTTGAGGCGTGTGAACAATAACGGCGTAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCAC                                                                                                            <  SRR19973065.143918/150‑1 (MQ=60)
                                               CGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTA                                                                                                        <  SRR19973065.3391621/150‑1 (MQ=60)
                                                GCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTAC                                                                                                       <  SRR19973065.285410/150‑1 (MQ=60)
                                                 CGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACC                                                                                                      >  SRR19973065.3228787/1‑150 (MQ=60)
                                                  GGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCA                                                                                                     <  SRR19973065.2020809/150‑1 (MQ=60)
                                                  GGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCA                                                                                                     <  SRR19973065.2810059/150‑1 (MQ=60)
                                                     GGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCG                                                                                                  <  SRR19973065.1709016/150‑1 (MQ=60)
                                                            TAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGA                                                                                           <  SRR19973065.431373/150‑1 (MQ=60)
                                                            TAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGA                                                                                           <  SRR19973065.430444/150‑1 (MQ=60)
                                                            TAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGA                                                                                           <  SRR19973065.431559/150‑1 (MQ=60)
                                                             AAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGAT                                                                                          >  SRR19973065.1840778/1‑150 (MQ=60)
                                                               ATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGT                                                                                        >  SRR19973065.2847245/1‑150 (MQ=60)
                                                               ATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGT                                                                                        >  SRR19973065.2847214/1‑150 (MQ=60)
                                                                 TCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTT                                                                                      <  SRR19973065.2164815/150‑1 (MQ=60)
                                                                    TGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTT                                                                                   >  SRR19973065.3167117/1‑150 (MQ=60)
                                                                      AGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCG                                                                                 <  SRR19973065.2038752/150‑1 (MQ=60)
                                                                      AGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCG                                                                                 <  SRR19973065.2039653/150‑1 (MQ=60)
                                                                      AGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCG                                                                                 <  SRR19973065.2039743/150‑1 (MQ=60)
                                                                        CCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCC                                                                               <  SRR19973065.1415655/150‑1 (MQ=60)
                                                                         CGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCC                                                                              >  SRR19973065.719483/1‑150 (MQ=60)
                                                                         CGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCC                                                                              >  SRR19973065.719480/1‑150 (MQ=60)
                                                                         CGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCC                                                                              >  SRR19973065.719051/1‑150 (MQ=60)
                                                                          GAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCA                                                                             <  SRR19973065.821677/150‑1 (MQ=60)
                                                                          GAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCA                                                                             <  SRR19973065.1866861/150‑1 (MQ=60)
                                                                          GAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCA                                                                             <  SRR19973065.1869630/150‑1 (MQ=60)
                                                                          GAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCA                                                                             <  SRR19973065.822080/150‑1 (MQ=60)
                                                                          GAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCA                                                                             <  SRR19973065.2893857/150‑1 (MQ=60)
                                                                           AGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAG                                                                            <  SRR19973065.97919/150‑1 (MQ=60)
                                                                           AGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAG                                                                            >  SRR19973065.1577533/1‑150 (MQ=60)
                                                                                  AATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGG                                                                     >  SRR19973065.3028063/1‑150 (MQ=60)
                                                                                  AATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGG                                                                     >  SRR19973065.1269527/1‑150 (MQ=60)
                                                                                             AATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCT                                                          <  SRR19973065.2378962/150‑1 (MQ=60)
                                                                                             AATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCT                                                          <  SRR19973065.435554/150‑1 (MQ=60)
                                                                                                  TATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTACCAC                                                     >  SRR19973065.3189643/1‑150 (MQ=60)
                                                                                                         CGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCG                                              >  SRR19973065.2474154/1‑150 (MQ=60)
                                                                                                         CGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCG                                              >  SRR19973065.896188/1‑150 (MQ=60)
                                                                                                              GTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAAT                                         >  SRR19973065.1108840/1‑150 (MQ=60)
                                                                                                                  CTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTG                                     <  SRR19973065.1293113/150‑1 (MQ=60)
                                                                                                                  CTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTG                                     <  SRR19973065.3048828/150‑1 (MQ=60)
                                                                                                                      CTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTT                                 >  SRR19973065.2840080/1‑150 (MQ=60)
                                                                                                                         GAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCA                              >  SRR19973065.1541380/1‑150 (MQ=60)
                                                                                                                         GAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCA                              >  SRR19973065.2789320/1‑150 (MQ=60)
                                                                                                                         GAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCA                              <  SRR19973065.787984/150‑1 (MQ=60)
                                                                                                                         GAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCA                              >  SRR19973065.1026110/1‑150 (MQ=60)
                                                                                                                             CCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCA                          <  SRR19973065.1663621/150‑1 (MQ=60)
                                                                                                                             CCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCA                          >  SRR19973065.373299/1‑150 (MQ=60)
                                                                                                                             CCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCA                          >  SRR19973065.373487/1‑150 (MQ=60)
                                                                                                                             CCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCA                          <  SRR19973065.1663624/150‑1 (MQ=60)
                                                                                                                             CCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCA                          >  SRR19973065.3339001/1‑150 (MQ=60)
                                                                                                                                GCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAG                       <  SRR19973065.3512722/150‑1 (MQ=60)
                                                                                                                                 CTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGG                      <  SRR19973065.2748502/150‑1 (MQ=60)
                                                                                                                                 CTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGG                      <  SRR19973065.2748550/150‑1 (MQ=60)
                                                                                                                                  TCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGG                     <  SRR19973065.2534503/150‑1 (MQ=60)
                                                                                                                                         GCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTA              <  SRR19973065.1318679/150‑1 (MQ=60)
                                                                                                                                          CGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAG             <  SRR19973065.768826/150‑1 (MQ=60)
                                                                                                                                           GGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGA            >  SRR19973065.3372471/1‑150 (MQ=60)
                                                                                                                                            GGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTCGAT           >  SRR19973065.2714755/1‑150 (MQ=60)
                                                                                                                                            GGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGAT           <  SRR19973065.1809377/150‑1 (MQ=60)
                                                                                                                                            GGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTAGCCCAGGCGCTGGTAACCCATCCTTCCAACATGGCGGTAATCGTGCCTTTCACCCAAATGGCGGCGGAGAT           >  SRR19973065.2397948/1‑150 (MQ=60)
                                                                                                                                             GATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATA          <  SRR19973065.2957951/150‑1 (MQ=60)
                                                                                                                                                  TGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTGGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCAC     <  SRR19973065.1414313/150‑1 (MQ=60)
                                                                                                                                                    CTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCA   >  SRR19973065.1534942/1‑150 (MQ=60)
                                                                                                                                                    CTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCA   <  SRR19973065.179946/150‑1 (MQ=60)
                                                                                                                                                     TAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCAT  >  SRR19973065.1756988/1‑150 (MQ=60)
                                                                                                                                                     TAATACTCATTCAGCTTTCTTTTCCGGTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCAT  >  SRR19973065.1756028/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCAT  >  CP009273/4074430‑4074728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑