Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,074,579 G→T 100% R4S (CGC→AGC)  fdhE ← formate dehydrogenase formation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,074,5790GT98.8% 778.3 / 0.9 241R4S (CGC→AGC) fdhEformate dehydrogenase formation protein
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base T (123/115);  total (123/118)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 241 total aligned reads displayed.

TCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGC  >  CP009273/4074434‑4074723
                                                                                                                                                 |                                                                                                                                                
tCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTaa                                                                                                                                                >  1:233826/1‑148 (MQ=255)
 cACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAAt                                                                                                                                               <  2:49426/148‑1 (MQ=255)
 cACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAAt                                                                                                                                               <  2:2631288/148‑1 (MQ=255)
    ccAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACt                                                                                                                                            >  1:1049483/1‑148 (MQ=255)
     cAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTc                                                                                                                                           >  1:2809455/1‑148 (MQ=255)
     cAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTc                                                                                                                                           <  2:859710/148‑1 (MQ=255)
          gATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCa                                                                                                                                      >  2:1490297/1‑148 (MQ=255)
           attattTTCTGCCAGCTCGCGCAGACGCTGGGCGCGGCGTTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAg                                                                                                                                     <  1:3509310/148‑1 (MQ=255)
           attattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAg                                                                                                                                     >  1:176160/1‑148 (MQ=255)
            ttattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGc                                                                                                                                    <  2:3262028/148‑1 (MQ=255)
            ttattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGc                                                                                                                                    >  2:2871594/1‑148 (MQ=255)
            ttattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGc                                                                                                                                    <  2:244314/148‑1 (MQ=255)
             tattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGct                                                                                                                                   <  1:102374/148‑1 (MQ=255)
             tattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGct                                                                                                                                   >  2:1924207/1‑148 (MQ=255)
             tattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGct                                                                                                                                   <  2:222270/148‑1 (MQ=255)
             tattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGct                                                                                                                                   >  2:424093/1‑148 (MQ=255)
             tattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGct                                                                                                                                   <  1:3491452/148‑1 (MQ=255)
             tattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGct                                                                                                                                   <  1:3491406/148‑1 (MQ=255)
             tattGTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGct                                                                                                                                   <  2:2148682/148‑1 (MQ=255)
               ttttCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGcttt                                                                                                                                 >  1:917266/1‑148 (MQ=255)
               ttttCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGcttt                                                                                                                                 <  1:3520461/148‑1 (MQ=255)
                 ttCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttct                                                                                                                               <  2:2160757/148‑1 (MQ=255)
                  tCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttctt                                                                                                                              <  1:492806/148‑1 (MQ=255)
                   cTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttcttt                                                                                                                             <  1:1964416/148‑1 (MQ=255)
                    tGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCtttt                                                                                                                            <  2:3757615/148‑1 (MQ=255)
                     gCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTc                                                                                                                           <  1:2656956/148‑1 (MQ=255)
                     gCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTc                                                                                                                           >  1:3430329/1‑148 (MQ=255)
                       cAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCg                                                                                                                         <  1:845457/148‑1 (MQ=255)
                       cAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCg                                                                                                                         <  2:2636866/148‑1 (MQ=255)
                       cAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCg                                                                                                                         <  1:2759848/148‑1 (MQ=255)
                          cTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTg                                                                                                                      <  2:1268098/148‑1 (MQ=255)
                          cTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTg                                                                                                                      <  1:2886656/148‑1 (MQ=255)
                            cgcgCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTc                                                                                                                    >  1:1018838/1‑148 (MQ=255)
                             gcgcAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCt                                                                                                                   <  2:2806612/148‑1 (MQ=255)
                              cgcAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCtt                                                                                                                  <  2:115797/148‑1 (MQ=255)
                               gcAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTg                                                                                                                 <  2:2148777/148‑1 (MQ=255)
                                                cggTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCa                                                                                                >  2:2940557/1‑148 (MQ=255)
                                                      tataAATTCGTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCgg                                                                                          <  2:3724138/148‑1 (MQ=255)
                                                       ataAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCggg                                                                                         >  1:3145234/1‑148 (MQ=255)
                                                         aaaTTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTg                                                                                       >  1:614000/1‑148 (MQ=255)
                                                          aaTTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGa                                                                                      <  2:976713/148‑1 (MQ=255)
                                                          aaTTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGa                                                                                      <  1:2954694/148‑1 (MQ=255)
                                                            ttCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATg                                                                                    >  2:31443/1‑148 (MQ=255)
                                                             tCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGt                                                                                   <  2:567095/148‑1 (MQ=255)
                                                               ttGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGttt                                                                                 <  2:574007/148‑1 (MQ=255)
                                                                       aGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGccc                                                                         >  2:3374265/1‑148 (MQ=255)
                                                                        gggAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCa                                                                        >  1:398043/1‑148 (MQ=255)
                                                                        gggAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCa                                                                        >  2:122880/1‑148 (MQ=255)
                                                                           aaCAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGgc                                                                     >  1:384917/1‑148 (MQ=255)
                                                                           aaCAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGgc                                                                     >  1:2889847/1‑148 (MQ=255)
                                                                               aTAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTg                                                                 >  1:2995990/1‑148 (MQ=255)
                                                                                tAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTgg                                                                >  2:2618994/1‑148 (MQ=255)
                                                                                 aaCGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGt                                                               >  2:1712705/1‑148 (MQ=255)
                                                                                   cggcggAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTaa                                                             <  2:3643673/148‑1 (MQ=255)
                                                                                      cggAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAAccc                                                          <  2:552036/148‑1 (MQ=255)
                                                                                        gAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCAt                                                        >  2:655899/1‑148 (MQ=255)
                                                                                        gAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCAt                                                        <  1:2261418/148‑1 (MQ=255)
                                                                                        gAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCAt                                                        >  1:2112807/1‑148 (MQ=255)
                                                                                         aaTCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATc                                                       <  2:1103328/148‑1 (MQ=255)
                                                                                          aTCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATcc                                                      >  2:1394463/1‑148 (MQ=255)
                                                                                           tCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCt                                                     >  2:3086202/1‑148 (MQ=255)
                                                                                           tCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCt                                                     >  2:311018/1‑148 (MQ=255)
                                                                                            cATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCtt                                                    >  1:3003164/1‑148 (MQ=255)
                                                                                                 ccgccgTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTccacc                                               >  1:370844/1‑148 (MQ=255)
                                                                                                 ccgccgTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTccacc                                               >  2:1810809/1‑148 (MQ=255)
                                                                                                  cgccgTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCACCCTTccacca                                              <  2:917266/148‑1 (MQ=255)
                                                                                                    ccgTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATg                                            >  1:478050/1‑148 (MQ=255)
                                                                                                     cgTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATgg                                           <  2:146121/148‑1 (MQ=255)
                                                                                                       tACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCg                                         >  2:2059241/1‑148 (MQ=255)
                                                                                                       tACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCg                                         >  2:1521773/1‑148 (MQ=255)
                                                                                                           tttCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTaa                                     >  1:2166298/1‑148 (MQ=255)
                                                                                                           tttCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTaa                                     <  2:3430329/148‑1 (MQ=255)
                                                                                                            ttCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAAt                                    >  1:671523/1‑148 (MQ=255)
                                                                                                                cGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTg                                <  2:3564219/148‑1 (MQ=255)
                                                                                                                cGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTg                                >  2:3015984/1‑148 (MQ=255)
                                                                                                                 gCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGc                               <  1:1243761/148‑1 (MQ=255)
                                                                                                                 gCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGc                               <  1:1068832/148‑1 (MQ=255)
                                                                                                                  cTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGcc                              <  1:1490297/148‑1 (MQ=255)
                                                                                                                      aaCCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTc                          <  1:122880/148‑1 (MQ=255)
                                                                                                                        cccAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCAc                        >  2:1922722/1‑148 (MQ=255)
                                                                                                                         ccAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCAcc                       <  1:3593984/148‑1 (MQ=255)
                                                                                                                            gCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCaa                    <  1:1120708/148‑1 (MQ=255)
                                                                                                                            gCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCaa                    <  1:2968246/148‑1 (MQ=255)
                                                                                                                             cTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCaaa                   >  2:1854724/1‑148 (MQ=255)
                                                                                                                               cATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAgg                 <  1:1307230/148‑1 (MQ=255)
                                                                                                                                 tCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGggc               <  1:240285/148‑1 (MQ=255)
                                                                                                                                 tCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGggc               >  1:449450/1‑148 (MQ=255)
                                                                                                                                 tCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGggc               >  2:418212/1‑148 (MQ=255)
                                                                                                                                 tCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGggc               <  1:2169244/148‑1 (MQ=255)
                                                                                                                                        ggATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAg        >  2:815195/1‑148 (MQ=255)
                                                                                                                                        ggATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAg        >  1:1465612/1‑148 (MQ=255)
                                                                                                                                        ggATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAg        >  2:1341802/1‑148 (MQ=255)
                                                                                                                                        ggATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAg        <  1:1924207/148‑1 (MQ=255)
                                                                                                                                         gATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGa       >  1:3415955/1‑148 (MQ=255)
                                                                                                                                          aTTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGat      >  1:3247359/1‑148 (MQ=255)
                                                                                                                                          aTTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGat      >  1:1603057/1‑148 (MQ=255)
                                                                                                                                            tATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGatat    >  1:2746339/1‑148 (MQ=255)
                                                                                                                                            tATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGatat    >  1:831849/1‑148 (MQ=255)
                                                                                                                                              tGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGc  <  1:484582/148‑1 (MQ=255)
                                                                                                                                              tGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGc  <  1:1712705/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGC  >  CP009273/4074434‑4074723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCA  >  CP009273/4074430‑4074727
                                                                                                                                                     |                                                                                                                                                    
GTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCT                                                                                                                                                      <  SRR19973069.1367914/150‑1 (MQ=60)
  AATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAA                                                                                                                                                    >  SRR19973069.235509/1‑150 (MQ=60)
  AATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAA                                                                                                                                                    >  SRR19973069.235600/1‑150 (MQ=60)
  AATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAA                                                                                                                                                    >  SRR19973069.3184657/1‑150 (MQ=60)
      ACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACT                                                                                                                                                >  SRR19973069.1056768/1‑150 (MQ=60)
      ACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACT                                                                                                                                                <  SRR19973069.2895096/150‑1 (MQ=60)
       CCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTC                                                                                                                                               >  SRR19973069.2828074/1‑150 (MQ=60)
          AGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATT                                                                                                                                            >  SRR19973069.107323/1‑150 (MQ=60)
          AGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATT                                                                                                                                            <  SRR19973069.2838575/150‑1 (MQ=60)
             GGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAG                                                                                                                                         >  SRR19973069.177648/1‑150 (MQ=60)
             GGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAG                                                                                                                                         >  SRR19973069.714309/1‑150 (MQ=60)
               ATTATTTTCTGCCAGCTCGCGCAGACGCTGGGCGCGGCGTTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCT                                                                                                                                       <  SRR19973069.3531904/150‑1 (MQ=60)
               ATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCT                                                                                                                                       <  SRR19973069.3531842/150‑1 (MQ=60)
                 TATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTT                                                                                                                                     >  SRR19973069.923522/1‑150 (MQ=60)
                 TATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTT                                                                                                                                     <  SRR19973069.103399/150‑1 (MQ=60)
                 TATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTT                                                                                                                                     <  SRR19973069.3513921/150‑1 (MQ=60)
                 TATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTT                                                                                                                                     <  SRR19973069.3513953/150‑1 (MQ=60)
                 TATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTT                                                                                                                                     <  SRR19973069.3513967/150‑1 (MQ=60)
                   TTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCT                                                                                                                                   <  SRR19973069.3543206/150‑1 (MQ=60)
                   TTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCT                                                                                                                                   <  SRR19973069.3542455/150‑1 (MQ=60)
                      TCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTT                                                                                                                                <  SRR19973069.496350/150‑1 (MQ=60)
                       CTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTC                                                                                                                               <  SRR19973069.1978040/150‑1 (MQ=60)
                       CTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTC                                                                                                                               >  SRR19973069.3450429/1‑150 (MQ=60)
                       CTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTC                                                                                                                               >  SRR19973069.3452634/1‑150 (MQ=60)
                         GCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCG                                                                                                                             <  SRR19973069.2674765/150‑1 (MQ=60)
                           CAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTT                                                                                                                           <  SRR19973069.421162/150‑1 (MQ=60)
                           CAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTT                                                                                                                           <  SRR19973069.2778269/150‑1 (MQ=60)
                           CAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTT                                                                                                                           <  SRR19973069.851232/150‑1 (MQ=60)
                           CAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTT                                                                                                                           <  SRR19973069.851387/150‑1 (MQ=60)
                              CTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTC                                                                                                                        <  SRR19973069.2905666/150‑1 (MQ=60)
                              CTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTC                                                                                                                        >  SRR19973069.1025882/1‑150 (MQ=60)
                              CTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTC                                                                                                                        >  SRR19973069.2774554/1‑150 (MQ=60)
                              CTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTC                                                                                                                        <  SRR19973069.2907482/150‑1 (MQ=60)
                              CTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTC                                                                                                                        >  SRR19973069.1026057/1‑150 (MQ=60)
                                CGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTT                                                                                                                      <  SRR19973069.207495/150‑1 (MQ=60)
                                   GCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCG                                                                                                                   <  SRR19973069.710083/150‑1 (MQ=60)
                                     AGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGACCAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGA                                                                                                                 >  SRR19973069.3510486/1‑150 (MQ=60)
                                     AGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGA                                                                                                                 >  SRR19973069.1384107/1‑150 (MQ=60)
                                     AGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGA                                                                                                                 >  SRR19973069.1384280/1‑150 (MQ=60)
                                     AGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGA                                                                                                                 >  SRR19973069.2516115/1‑150 (MQ=60)
                                                     GGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCG                                                                                                 >  SRR19973069.2383625/1‑150 (MQ=60)
                                                         GTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGG                                                                                             >  SRR19973069.3464352/1‑150 (MQ=60)
                                                         GTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGG                                                                                             >  SRR19973069.3165810/1‑150 (MQ=60)
                                                           ATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTG                                                                                           >  SRR19973069.618104/1‑150 (MQ=60)
                                                           ATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTG                                                                                           >  SRR19973069.618177/1‑150 (MQ=60)
                                                              AATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATG                                                                                        <  SRR19973069.2974307/150‑1 (MQ=60)
                                                                 TCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTT                                                                                     >  SRR19973069.1286041/1‑150 (MQ=60)
                                                                     GAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTC                                                                                 <  SRR19973069.3016210/150‑1 (MQ=60)
                                                                         CGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCC                                                                             >  SRR19973069.1115835/1‑150 (MQ=60)
                                                                          GAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCA                                                                            >  SRR19973069.400433/1‑150 (MQ=60)
                                                                          GAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCA                                                                            >  SRR19973069.400951/1‑150 (MQ=60)
                                                                             GGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGC                                                                         >  SRR19973069.388582/1‑150 (MQ=60)
                                                                             GGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGC                                                                         >  SRR19973069.387738/1‑150 (MQ=60)
                                                                             GGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGC                                                                         >  SRR19973069.496946/1‑150 (MQ=60)
                                                                             GGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGC                                                                         >  SRR19973069.496947/1‑150 (MQ=60)
                                                                             GGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGC                                                                         >  SRR19973069.2908902/1‑150 (MQ=60)
                                                                             GGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGC                                                                         >  SRR19973069.2908893/1‑150 (MQ=60)
                                                                              GAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCG                                                                        >  SRR19973069.2818950/1‑150 (MQ=60)
                                                                                ACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCT                                                                      >  SRR19973069.3527459/1‑150 (MQ=60)
                                                                                 CAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTG                                                                     <  SRR19973069.10614/150‑1 (MQ=60)
                                                                                 CAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTG                                                                     >  SRR19973069.3015877/1‑150 (MQ=60)
                                                                                  AATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGG                                                                    >  SRR19973069.856452/1‑150 (MQ=60)
                                                                                  AATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGG                                                                    <  SRR19973069.3101204/150‑1 (MQ=60)
                                                                                        GGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCC                                                              >  SRR19973069.2170362/1‑150 (MQ=60)
                                                                                          CGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCAT                                                            >  SRR19973069.2127375/1‑150 (MQ=60)
                                                                                            GAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCC                                                          <  SRR19973069.2276723/150‑1 (MQ=60)
                                                                                              ATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTT                                                        >  SRR19973069.3023090/1‑150 (MQ=60)
                                                                                              ATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTT                                                        >  SRR19973069.3021660/1‑150 (MQ=60)
                                                                                                CATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCC                                                      >  SRR19973069.3545940/1‑150 (MQ=60)
                                                                                                  TATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCAC                                                    <  SRR19973069.293253/150‑1 (MQ=60)
                                                                                                  TATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCAC                                                    <  SRR19973069.2234037/150‑1 (MQ=60)
                                                                                                   ATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACC                                                   >  SRR19973069.373550/1‑150 (MQ=60)
                                                                                                   ATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACC                                                   >  SRR19973069.372860/1‑150 (MQ=60)
                                                                                                      CGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTCACCCATCCTTCCACCATG                                                >  SRR19973069.481459/1‑150 (MQ=60)
                                                                                                      CGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATG                                                >  SRR19973069.1812169/1‑150 (MQ=60)
                                                                                                      CGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATG                                                >  SRR19973069.481518/1‑150 (MQ=60)
                                                                                                         CGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCG                                             >  SRR19973069.221246/1‑150 (MQ=60)
                                                                                                           TACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGT                                           <  SRR19973069.1899448/150‑1 (MQ=60)
                                                                                                            ACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTA                                          <  SRR19973069.1182082/150‑1 (MQ=60)
                                                                                                             CGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAA                                         >  SRR19973069.2181107/1‑150 (MQ=60)
                                                                                                             CGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAA                                         >  SRR19973069.1638294/1‑150 (MQ=60)
                                                                                                              GTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAAT                                        >  SRR19973069.3622001/1‑150 (MQ=60)
                                                                                                              GTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAAT                                        >  SRR19973069.676142/1‑150 (MQ=60)
                                                                                                                  CTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACTGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTG                                    >  SRR19973069.3827387/1‑150 (MQ=60)
                                                                                                                     GCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCT                                 <  SRR19973069.1252211/150‑1 (MQ=60)
                                                                                                                     GCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCT                                 <  SRR19973069.1251797/150‑1 (MQ=60)
                                                                                                                     GCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCT                                 <  SRR19973069.1076283/150‑1 (MQ=60)
                                                                                                                      CTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTT                                <  SRR19973069.1500647/150‑1 (MQ=60)
                                                                                                                       TTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTT                               <  SRR19973069.492222/150‑1 (MQ=60)
                                                                                                                       TTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTT                               <  SRR19973069.1956952/150‑1 (MQ=60)
                                                                                                                         GAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCGTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCA                             <  SRR19973069.3397964/150‑1 (MQ=60)
                                                                                                                          AACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCAC                            <  SRR19973069.124044/150‑1 (MQ=60)
                                                                                                                          AACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCAC                            <  SRR19973069.123999/150‑1 (MQ=60)
                                                                                                                            CCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCC                          >  SRR19973069.1075444/1‑150 (MQ=60)
                                                                                                                             CCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCA                         <  SRR19973069.3617446/150‑1 (MQ=60)
                                                                                                                              CAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAA                        >  SRR19973069.2043848/1‑150 (MQ=60)
                                                                                                                                GCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCTCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAG                      <  SRR19973069.2987978/150‑1 (MQ=60)
                                                                                                                                GCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAG                      <  SRR19973069.1128520/150‑1 (MQ=60)
                                                                                                                                GCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAG                      <  SRR19973069.2987949/150‑1 (MQ=60)
                                                                                                                                GCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAG                      <  SRR19973069.2987856/150‑1 (MQ=60)
                                                                                                                                  TCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGG                    >  SRR19973069.1585064/1‑150 (MQ=60)
                                                                                                                                   CATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGC                   <  SRR19973069.1316150/150‑1 (MQ=60)
                                                                                                                                   CATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGC                   >  SRR19973069.452738/1‑150 (MQ=60)
                                                                                                                                   CATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGC                   >  SRR19973069.452742/1‑150 (MQ=60)
                                                                                                                                     TCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGG                 <  SRR19973069.242076/150‑1 (MQ=60)
                                                                                                                                     TCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGG                 <  SRR19973069.2183221/150‑1 (MQ=60)
                                                                                                                                     TCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGG                 <  SRR19973069.2184060/150‑1 (MQ=60)
                                                                                                                                     TCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGG                 <  SRR19973069.242086/150‑1 (MQ=60)
                                                                                                                                          CGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAG            >  SRR19973069.1475839/1‑150 (MQ=60)
                                                                                                                                          CGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAG            >  SRR19973069.1478938/1‑150 (MQ=60)
                                                                                                                                          CGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAG            >  SRR19973069.2762892/1‑150 (MQ=60)
                                                                                                                                          CGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAG            >  SRR19973069.2745023/1‑150 (MQ=60)
                                                                                                                                           GGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGA           >  SRR19973069.3438207/1‑150 (MQ=60)
                                                                                                                                           GGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGA           >  SRR19973069.3230723/1‑150 (MQ=60)
                                                                                                                                           GGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGA           >  SRR19973069.1525189/1‑150 (MQ=60)
                                                                                                                                            GGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGAT          >  SRR19973069.3268620/1‑150 (MQ=60)
                                                                                                                                            GGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGAT          >  SRR19973069.1613852/1‑150 (MQ=60)
                                                                                                                                            GGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGAT          <  SRR19973069.1937433/150‑1 (MQ=60)
                                                                                                                                              ATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATAT        >  SRR19973069.837700/1‑150 (MQ=60)
                                                                                                                                              ATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATAT        >  SRR19973069.2764687/1‑150 (MQ=60)
                                                                                                                                                  TGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCAC    <  SRR19973069.488090/150‑1 (MQ=60)
                                                                                                                                                  TGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCAC    <  SRR19973069.488079/150‑1 (MQ=60)
                                                                                                                                                  TGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCAC    <  SRR19973069.1724706/150‑1 (MQ=60)
                                                                                                                                                  TGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCAC    <  SRR19973069.1725631/150‑1 (MQ=60)
                                                                                                                                                    CTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCA  >  SRR19973069.1182040/1‑150 (MQ=60)
                                                                                                                                                    CTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCA  >  SRR19973069.1289531/1‑150 (MQ=60)
                                                                                                                                                    CTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCA  >  SRR19973069.3325068/1‑150 (MQ=60)
                                                                                                                                                    CTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCA  >  SRR19973069.3324992/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCA  >  CP009273/4074430‑4074727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑