Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,075,234 C→A 100% D296Y (GAC→TAC)  fdoH ← formate dehydrogenase‑O, Fe‑S subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,075,2340CA98.4% 578.2 / 0.7 183D296Y (GAC→TAC) fdoHformate dehydrogenase‑O, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base A (73/107);  total (73/110)
Fisher's exact test for biased strand distribution p-value = 2.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 183 total aligned reads displayed.

ATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTCTTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTC  >  CP009273/4075090‑4075378
                                                                                                                                                |                                                                                                                                                
 tCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTtc                                                                                                                                              <  1:1361790/148‑1 (MQ=255)
  cAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTtct                                                                                                                                             <  2:1382143/148‑1 (MQ=255)
  cAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTtct                                                                                                                                             <  1:3225683/148‑1 (MQ=255)
   aaCCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTtctc                                                                                                                                            >  2:1800543/1‑148 (MQ=255)
    aCCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTctct                                                                                                                                           >  2:1146263/1‑148 (MQ=255)
       aGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCg                                                                                                                                        >  1:2695394/1‑148 (MQ=255)
       aGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCg                                                                                                                                        >  1:2695999/1‑148 (MQ=255)
        gTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGt                                                                                                                                       >  1:521318/1‑148 (MQ=255)
          tGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGc                                                                                                                                     <  2:1566259/148‑1 (MQ=255)
            aaGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAg                                                                                                                                   >  1:1859267/1‑148 (MQ=255)
              ggACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGat                                                                                                                                 >  2:2584506/1‑148 (MQ=255)
              ggACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGat                                                                                                                                 <  1:2173061/148‑1 (MQ=255)
              ggACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGat                                                                                                                                 >  2:3052451/1‑148 (MQ=255)
              ggACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGat                                                                                                                                 >  1:883996/1‑148 (MQ=255)
                aCGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGatta                                                                                                                               >  2:222833/1‑148 (MQ=255)
                    gAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATtct                                                                                                                           <  2:730786/148‑1 (MQ=255)
                    gAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATtct                                                                                                                           <  1:1439460/148‑1 (MQ=255)
                     aaCAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATtctc                                                                                                                          <  1:553341/148‑1 (MQ=255)
                     aaCAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATtctc                                                                                                                          <  2:1785896/148‑1 (MQ=255)
                      aCAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTctct                                                                                                                         <  2:2528768/148‑1 (MQ=255)
                      aCAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTctct                                                                                                                         <  2:2528765/148‑1 (MQ=255)
                        aaaaaGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTc                                                                                                                       <  2:2933616/148‑1 (MQ=255)
                         aaaaGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTcc                                                                                                                      <  2:117219/148‑1 (MQ=255)
                           aaGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTc                                                                                                                    <  2:3363533/148‑1 (MQ=255)
                             gcccagcccGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGAGGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCAt                                                                                                                  >  2:728082/1‑148 (MQ=255)
                                   cccGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACTTCGTTTCATTTGAGCTACTAGTATTTCTCTTAGTGCACATTATTATATTACTCATCCGccc                                                                                                            >  1:2736251/1‑146 (MQ=255)
                                       cTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTCTTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGtt                                                                                                        <  1:2415298/148‑1 (MQ=255)
                                                  cAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGacac                                                                                             >  1:860178/1‑148 (MQ=255)
                                                  cAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGacac                                                                                             >  1:1706693/1‑148 (MQ=255)
                                                  cAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGacac                                                                                             >  1:2771429/1‑148 (MQ=255)
                                                    ggATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACAccc                                                                                           >  2:1335317/1‑148 (MQ=255)
                                                     gATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCCTCCGCACGGTTCGGACCGACACCCa                                                                                          >  1:2933726/1‑148 (MQ=255)
                                                     gATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCa                                                                                          <  2:2554602/148‑1 (MQ=255)
                                                        gAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGt                                                                                       >  1:2384764/1‑148 (MQ=255)
                                                           gCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGt                                                                                    <  2:2323398/148‑1 (MQ=255)
                                                           gCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGt                                                                                    <  2:2323591/148‑1 (MQ=255)
                                                           gCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGt                                                                                    >  1:1000969/1‑148 (MQ=255)
                                                           gCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGt                                                                                    <  2:536212/148‑1 (MQ=255)
                                                             aGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTgg                                                                                  <  1:3032215/148‑1 (MQ=255)
                                                             aGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTgg                                                                                  <  2:272479/148‑1 (MQ=255)
                                                             aGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTgg                                                                                  <  1:3428302/148‑1 (MQ=255)
                                                              gAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCCGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGa                                                                                 >  2:3257868/1‑148 (MQ=255)
                                                              gAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGa                                                                                 <  1:1775094/148‑1 (MQ=255)
                                                               aaGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGaa                                                                                <  2:1406249/148‑1 (MQ=255)
                                                               aaGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGaa                                                                                <  1:321476/148‑1 (MQ=255)
                                                               aaGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGaa                                                                                <  1:2027299/148‑1 (MQ=255)
                                                                aGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAg                                                                               <  1:940525/148‑1 (MQ=255)
                                                                aGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAg                                                                               <  2:1683548/148‑1 (MQ=255)
                                                                 ggCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGa                                                                              >  2:2567315/1‑148 (MQ=255)
                                                                  gCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGAt                                                                             >  1:3555811/1‑148 (MQ=255)
                                                                    ggTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATAc                                                                           <  2:2485008/148‑1 (MQ=255)
                                                                     gTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACt                                                                          <  2:1285002/148‑1 (MQ=255)
                                                                            aGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCAATTGCGCTCCTCGTATTTCTATTCGTGCAGATTATTCTCTTCCTCATCCCCACCCTTCGGACCGCCACCCACGTAGTGGAAGATACTGGCCGCa                                                                   >  1:389941/1‑148 (MQ=255)
                                                                              tGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTCTTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCaaa                                                                 <  1:579482/148‑1 (MQ=255)
                                                                              tGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCaaa                                                                 <  2:2728085/148‑1 (MQ=255)
                                                                                  tgctACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTa                                                             <  2:884690/145‑1 (MQ=255)
                                                                                  tGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTa                                                             <  1:3303525/148‑1 (MQ=255)
                                                                                  tGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTa                                                             >  2:3411413/1‑148 (MQ=255)
                                                                                  tGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTa                                                             <  1:1199649/148‑1 (MQ=255)
                                                                                       cGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTACTCAAACGCACGGTTAGGACCGACACCCACGTAGTTGAAGATAATGGCCGCAAAGGTAGCCGc                                                        >  1:3135453/1‑148 (MQ=255)
                                                                                         ttCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCAATTGCGCTCCTCGTATTTCTCTTAGTGCAGATTAATCTATTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCaa                                                      >  1:895649/1‑148 (MQ=255)
                                                                                          tCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCACAGGTAGCCGCaaa                                                     >  1:2304024/1‑148 (MQ=255)
                                                                                          tCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCaaa                                                     >  2:1990116/1‑148 (MQ=255)
                                                                                              gcgcGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCa                                                 <  1:193262/148‑1 (MQ=255)
                                                                                              gcgcGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTATTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCa                                                 <  1:672110/148‑1 (MQ=255)
                                                                                                gcgGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAAc                                               <  1:1647580/148‑1 (MQ=255)
                                                                                                  ggTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAg                                             <  2:521318/148‑1 (MQ=255)
                                                                                                    tgtAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTACTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGcc                                           >  2:378289/1‑148 (MQ=255)
                                                                                                      tAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCcgc                                         >  2:580401/1‑148 (MQ=255)
                                                                                                       aGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCcgcg                                        <  1:2425871/148‑1 (MQ=255)
                                                                                                         cgcACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAg                                      <  2:1096054/148‑1 (MQ=255)
                                                                                                         cgcACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAg                                      <  2:1097673/148‑1 (MQ=255)
                                                                                                          gcACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGc                                     <  2:448361/148‑1 (MQ=255)
                                                                                                            aCGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCgg                                   <  1:1800543/148‑1 (MQ=255)
                                                                                                             cGATGTTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGt                                  >  1:2665187/1‑148 (MQ=255)
                                                                                                             cGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGt                                  <  1:217377/148‑1 (MQ=255)
                                                                                                               aTGGTGTCACGTCGTTTCATTTGCGCTCCTCGTAGTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGttt                                <  2:2181950/148‑1 (MQ=255)
                                                                                                                tGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTc                               <  1:1091097/148‑1 (MQ=255)
                                                                                                                tGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTc                               <  2:2892340/148‑1 (MQ=255)
                                                                                                                 ggTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTcc                              <  2:1941470/148‑1 (MQ=255)
                                                                                                                 ggTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTcc                              >  2:63384/1‑148 (MQ=255)
                                                                                                                 ggTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTcc                              <  1:2550357/148‑1 (MQ=255)
                                                                                                                      cACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATg                         <  2:1911939/148‑1 (MQ=255)
                                                                                                                      cACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATg                         <  2:1778777/148‑1 (MQ=255)
                                                                                                                        cgtcgtTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGcc                       <  1:3367503/148‑1 (MQ=255)
                                                                                                                        cgtcgtTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGcc                       <  1:2351345/148‑1 (MQ=255)
                                                                                                                         gtcgtTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCt                      >  2:1496604/1‑148 (MQ=255)
                                                                                                                          tcgtTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCtt                     <  2:1797468/148‑1 (MQ=255)
                                                                                                                           cgtTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCttt                    <  2:2949823/148‑1 (MQ=255)
                                                                                                                           cgtTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCttt                    <  1:2584506/148‑1 (MQ=255)
                                                                                                                            gtTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTc                   <  1:3052451/148‑1 (MQ=255)
                                                                                                                             tttGATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTcc                  <  2:2517181/148‑1 (MQ=255)
                                                                                                                             tttCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTcc                  >  1:674503/1‑148 (MQ=255)
                                                                                                                             tttCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTcc                  <  1:2844985/148‑1 (MQ=255)
                                                                                                                                 aTTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGaa              <  2:2252264/148‑1 (MQ=255)
                                                                                                                                  tttGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAAt             <  2:1129707/148‑1 (MQ=255)
                                                                                                                                          cctcGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGt     <  1:2567315/148‑1 (MQ=255)
                                                                                                                                          cctcGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGt     <  1:2821801/148‑1 (MQ=255)
                                                                                                                                          cctcGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGt     >  1:678431/1‑148 (MQ=255)
                                                                                                                                          cctcGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGt     <  2:2638928/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTCTTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTC  >  CP009273/4075090‑4075378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTCTTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTCGCTG  >  CP009273/4075086‑4075382
                                                                                                                                                    |                                                                                                                                                    
TTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCCCGTAT                                                                                                                                                     <  SRR19973067.607165/150‑1 (MQ=60)
  GCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTT                                                                                                                                                   <  SRR19973067.1292436/150‑1 (MQ=60)
     TCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTC                                                                                                                                                <  SRR19973067.1371094/150‑1 (MQ=60)
      CAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTTCGCTCCTCGTATTTCTCT                                                                                                                                               >  SRR19973067.2714820/1‑150 (MQ=60)
      CAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCT                                                                                                                                               <  SRR19973067.3245944/150‑1 (MQ=60)
         CNAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCG                                                                                                                                            >  SRR19973067.2712730/1‑150 (MQ=60)
         CCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCG                                                                                                                                            >  SRR19973067.2713340/1‑150 (MQ=60)
          CAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGT                                                                                                                                           >  SRR19973067.524841/1‑150 (MQ=60)
          CAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGGATTTCTCTTCGT                                                                                                                                           >  SRR19973067.524975/1‑150 (MQ=60)
           AGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTG                                                                                                                                          <  SRR19973067.3413808/150‑1 (MQ=60)
              TGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAG                                                                                                                                       <  SRR19973067.3138156/150‑1 (MQ=60)
              TGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAG                                                                                                                                       >  SRR19973067.1871938/1‑150 (MQ=60)
               GAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGA                                                                                                                                      >  SRR19973067.1803787/1‑150 (MQ=60)
                AAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGAT                                                                                                                                     >  SRR19973067.2267218/1‑150 (MQ=60)
                AAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGAT                                                                                                                                     <  SRR19973067.503106/150‑1 (MQ=60)
                AAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGAT                                                                                                                                     >  SRR19973067.889875/1‑150 (MQ=60)
                 AGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATT                                                                                                                                    <  SRR19973067.3232463/150‑1 (MQ=60)
                  GGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTA                                                                                                                                   <  SRR19973067.2187591/150‑1 (MQ=60)
                        GAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCT                                                                                                                             <  SRR19973067.1449147/150‑1 (MQ=60)
                         AACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTT                                                                                                                            <  SRR19973067.556980/150‑1 (MQ=60)
                           CAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCC                                                                                                                          <  SRR19973067.1852429/150‑1 (MQ=60)
                            AAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGGTCCTCGTATTTCTCTTCGTGCAGGTTATTCTCTTCCT                                                                                                                         >  SRR19973067.78860/1‑150 (MQ=60)
                                     AGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACTTCGTTTCATTTGAGCTACTAGTATTTCTCTTAGTGCACATTATTATATTACTCATCCGCCC                                                                                                                >  SRR19973067.2753917/1‑150 (MQ=60)
                                     AGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTTCGCTCCTCGTATTTCTCTTCGTGCAGATTATTATCTTCCTCATCCGCAC                                                                                                                >  SRR19973067.650811/1‑150 (MQ=60)
                                         CGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTT                                                                                                            >  SRR19973067.2692154/1‑150 (MQ=60)
                                           CTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTCTTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCG                                                                                                          <  SRR19973067.2431115/150‑1 (MQ=60)
                                                    GCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACAC                                                                                                 >  SRR19973067.2657817/1‑150 (MQ=60)
                                                    GCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACAC                                                                                                 >  SRR19973067.865955/1‑150 (MQ=60)
                                                    GCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACAC                                                                                                 >  SRR19973067.2789330/1‑150 (MQ=60)
                                                    GCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACAC                                                                                                 >  SRR19973067.1718399/1‑150 (MQ=60)
                                                       AGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCCTCCGCACGGTTCGGACCGACACCCA                                                                                              >  SRR19973067.2952347/1‑150 (MQ=60)
                                                          ATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGT                                                                                           >  SRR19973067.2400183/1‑150 (MQ=60)
                                                          ATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGT                                                                                           >  SRR19973067.2400243/1‑150 (MQ=60)
                                                           TGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTA                                                                                          <  SRR19973067.92632/150‑1 (MQ=60)
                                                             AAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGT                                                                                        >  SRR19973067.1007851/1‑150 (MQ=60)
                                                              AGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTGTCATTTTCGCTCCTCGTAATTCTCATCGTGCAGATTATTGTCTTCCTCATACCCACGGTTCGGACCGACACCCACCGAGTG                                                                                       >  SRR19973067.1875394/1‑150 (MQ=60)
                                                               GCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGG                                                                                      <  SRR19973067.882851/150‑1 (MQ=60)
                                                                 AGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAA                                                                                    <  SRR19973067.3051526/150‑1 (MQ=60)
                                                                 AGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAA                                                                                    <  SRR19973067.3450118/150‑1 (MQ=60)
                                                                  GAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAG                                                                                   <  SRR19973067.1787122/150‑1 (MQ=60)
                                                                  GAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAG                                                                                   <  SRR19973067.1777949/150‑1 (MQ=60)
                                                                   AAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGA                                                                                  <  SRR19973067.323657/150‑1 (MQ=60)
                                                                   AAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGA                                                                                  <  SRR19973067.324010/150‑1 (MQ=60)
                                                                   AAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGA                                                                                  <  SRR19973067.2040901/150‑1 (MQ=60)
                                                                    AGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGAT                                                                                 <  SRR19973067.2777188/150‑1 (MQ=60)
                                                                    AGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGAT                                                                                 >  SRR19973067.3578219/1‑150 (MQ=60)
                                                                    AGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGAT                                                                                 <  SRR19973067.946932/150‑1 (MQ=60)
                                                                              CCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGGATTTATCTTCGTGCAGATTATTCTCTTACTCATCCGCACGGTTCGGACCGACACCCACCTAGTGGAAGATACTGGCCGCA                                                                       >  SRR19973067.392621/1‑150 (MQ=60)
                                                                              CCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCAATTGCGCTCCTCGTATTTCTATTCGTGCAGATTATTCTCTTCCTCATCCCCACCCTTCGGACCGCCACCCACGTAGTGGAAGATACTGGCCGCA                                                                       >  SRR19973067.392660/1‑150 (MQ=60)
                                                                                 GTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAG                                                                    <  SRR19973067.2561228/150‑1 (MQ=60)
                                                                                  TGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTCTTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGG                                                                   <  SRR19973067.583204/150‑1 (MQ=60)
                                                                                      TGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGC                                                               <  SRR19973067.3324106/150‑1 (MQ=60)
                                                                                      TGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGC                                                               <  SRR19973067.1207497/150‑1 (MQ=60)
                                                                                      TGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGC                                                               <  SRR19973067.3324352/150‑1 (MQ=60)
                                                                                        ATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTAGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTTGCCG                                                             <  SRR19973067.2362485/150‑1 (MQ=60)
                                                                                         TACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGC                                                            <  SRR19973067.2571215/150‑1 (MQ=60)
                                                                                         TACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTACTCAAACGCACGGTTAGGACCGACACCCACGTAGTTGAAGATAATGGCCGCAAAGGTAGCCGC                                                            >  SRR19973067.3155367/1‑150 (MQ=60)
                                                                                           CGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCAATTGCGCTCCTCGTATTTCTCTTAGTGCAGATTAATCTATTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAA                                                          >  SRR19973067.901563/1‑150 (MQ=60)
                                                                                            GTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCACAGGTAGCCGCAAA                                                         >  SRR19973067.2319206/1‑150 (MQ=60)
                                                                                            GTACCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTACAATTGCGCTACTCGTATTTATATTCGTGCAGAATATTCACTTCCTCATCCGCACGGTGCCGAACGACAACCAAGTAGTGGAAGATACTGTCCGCAAACGTAGCCGAAAA                                                         >  SRR19973067.2319176/1‑150 (MQ=60)
                                                                                                  GCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAAC                                                   <  SRR19973067.194755/150‑1 (MQ=60)
                                                                                                  GCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTATTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAAC                                                   <  SRR19973067.676711/150‑1 (MQ=60)
                                                                                                   CGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACA                                                  <  SRR19973067.1394360/150‑1 (MQ=60)
                                                                                                    GCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAG                                                 <  SRR19973067.1658925/150‑1 (MQ=60)
                                                                                                     CGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGC                                                >  SRR19973067.242209/1‑150 (MQ=60)
                                                                                                           AGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAG                                          <  SRR19973067.2441787/150‑1 (MQ=60)
                                                                                                               CACGATGTTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGT                                      >  SRR19973067.2682315/1‑150 (MQ=60)
                                                                                                                ACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTT                                     <  SRR19973067.1812637/150‑1 (MQ=60)
                                                                                                                 CGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTT                                    <  SRR19973067.218977/150‑1 (MQ=60)
                                                                                                                 CGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTT                                    <  SRR19973067.218951/150‑1 (MQ=60)
                                                                                                                 CGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTT                                    <  SRR19973067.2026413/150‑1 (MQ=60)
                                                                                                                    TGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCA                                 <  SRR19973067.1098545/150‑1 (MQ=60)
                                                                                                                     GGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAG                                <  SRR19973067.2567038/150‑1 (MQ=60)
                                                                                                                            CGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTT                         <  SRR19973067.3388884/150‑1 (MQ=60)
                                                                                                                            CGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTT                         <  SRR19973067.2366695/150‑1 (MQ=60)
                                                                                                                            CGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTT                         >  SRR19973067.1485812/1‑150 (MQ=60)
                                                                                                                             GTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTT                        <  SRR19973067.2839816/150‑1 (MQ=60)
                                                                                                                             GTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTT                        <  SRR19973067.2840732/150‑1 (MQ=60)
                                                                                                                               CGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCC                      <  SRR19973067.2601336/150‑1 (MQ=60)
                                                                                                                               CGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCC                      >  SRR19973067.679135/1‑150 (MQ=60)
                                                                                                                               CGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCC                      >  SRR19973067.678970/1‑150 (MQ=60)
                                                                                                                                GTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCA                     <  SRR19973067.3071900/150‑1 (MQ=60)
                                                                                                                                GTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCA                     <  SRR19973067.1270482/150‑1 (MQ=60)
                                                                                                                                GTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCA                     >  SRR19973067.3451642/1‑150 (MQ=60)
                                                                                                                                 TTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAG                    <  SRR19973067.2863246/150‑1 (MQ=60)
                                                                                                                                 TTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAG                    <  SRR19973067.2863784/150‑1 (MQ=60)
                                                                                                                                 TTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAG                    <  SRR19973067.1141744/150‑1 (MQ=60)
                                                                                                                                            CTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGT         >  SRR19973067.683086/1‑150 (MQ=60)
                                                                                                                                            CTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGT         >  SRR19973067.683088/1‑150 (MQ=60)
                                                                                                                                              CCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTT       <  SRR19973067.2839960/150‑1 (MQ=60)
                                                                                                                                              CCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTT       <  SRR19973067.2584076/150‑1 (MQ=60)
                                                                                                                                              CCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTT       <  SRR19973067.2584192/150‑1 (MQ=60)
                                                                                                                                               CTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTC      >  SRR19973067.3053795/1‑150 (MQ=60)
                                                                                                                                                   TATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTCGCTG  <  SRR19973067.2383340/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
TTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTCTTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTCGCTG  >  CP009273/4075086‑4075382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑